MD03G1288300.v1.1

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006691
Physical Location & Seq
Reverse (-)
36701304 .. 36707923
6620 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1288300.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGAGGGAGAATATCAAGAAGTTTACATAAGGAATTCAAGAGGAGTGGAGCTTTTTACTTGCAGATGGTTGCCCTTTTCATCTCCAAAGGCCCTTGTTTTTCTTTGCCACGGGTATGGCATGGAGTGCAGCGGTTTCATGAGAGAGTGTGGGATTCGCCTAGCTAACGCGGGCTATGCCGTGTTTGGAATCGACTACGAGGGCCACGGACGTTCGAGAGGGGCTAGATGTTACATCAAGAAGTTCGAAAACATAGTCAACGATTGCAATGAATTCTTCAAATCAATTAGTGCGGAGGAGGAGTACCGTAACAAGTGCAGATTTTTGTACGGAGAATCCATGGGAGGGGCAGTTTCCCTGTTGCTTCACAAGAAGGATCCCACATTTTGGAATGGTGCTGTTCTTGTTGCACCCATGTGTAAGATATCAGAAAAGGTCAAGCCACACCCGCTGGTGATCAATGTATTAATCAGAGTGGTGGAGATTATTCCCAAATGGAAGATAGTACCCACCAAGGACGTCATTGATTCCGCCTTCAAAGATCCTGTAAAGCGCGAAAAGATAAGGAGCAACAAACTGATATATCAAGACAAGCCAAGGCTTAAAACAGCACTGGAAATGCTGAGAACTAGCATGACCCTTGAAGACACTTTGCATGAGGTGAGGCTACCATTCTTCGTGTTGCATGGGGAGGCAGATACAGTTACAGACCCAGAAGTGAGCAGATCACTGTATGAGAAAGCCAGCAGCACAGACAAGACCATAAAACTGTACCCTGGAATGTGGCACGGCCTAACGTCGGGAGAGCCCGATGGGAACGTGGAGGTCGTCTTTGCAGACATCATAACTTGGCTCGAGAAGCACACGAGTGATGACAACTCCGTCGTGGTGCAACCAATCCACACGTTTAAGCATGGCATTCTGAAAATGAACGGCACGGCATCTCCACCATCCCAGACACCCAAAAAAAAGCAGCGACCGCGGAAGCCATCATATTTTTGTGGGTTGAGAGGGCGCAGATTGCTACATCACTCGCCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 ko05225 map00561 map01100 map04714 map04723 map04923 map05225
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

348

Amino Acids

39.53

Weight (kDa)

9.13

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 28 - 270 3.4e-65 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 32 - 149 5.7e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 33 - 265 5.6e-08 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 881
AatII GACGTC 1 cut(s) 522
AccB7I CCANNNNNTGG 1 cut(s) 387
AccII CGCG 3 cut(s) 170, 555, 982
AciI CCGC 7 cut(s) 132, 170, 293, 449, 531, 980, 982
AclWI GGATC 3 cut(s) 371, 384, 536
AcsI RAATTY 2 cut(s) 34, 272
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 4 cut(s) 305, 329, 507, 773
AfiI CCNNNNNNNGG 3 cut(s) 345, 387, 799
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 4 cut(s) 39, 280, 538, 644
AjnI CCWGG 1 cut(s) 775
AleI CACNNNNGTG 2 cut(s) 415, 867
AluBI AGCT 2 cut(s) 52, 164
AluI AGCT 2 cut(s) 52, 164
AlwI GGATC 3 cut(s) 371, 384, 536
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 3 cut(s) 90, 202, 790
ApeKI GCWGC 3 cut(s) 129, 747, 973
ApoI RAATTY 2 cut(s) 34, 272
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AseI ATTAAT 1 cut(s) 467
AspLEI GCGC 2 cut(s) 555, 1017
AspS9I GGNCC 2 cut(s) 91, 202
AsuHPI GGTGA 2 cut(s) 466, 673
AsuII TTCGAA 1 cut(s) 246
AvaI CYCGRG 1 cut(s) 854
BamHI GGATCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 810
BauI CACGAG 1 cut(s) 865
BbsI GAAGAC 1 cut(s) 651
BbvI GCAGC 3 cut(s) 141, 759, 985
BccI CCATC 4 cut(s) 60, 806, 958, 997
BceAI ACGGC 4 cut(s) 164, 805, 949, 954
BciT130I CCWGG 1 cut(s) 777
BclI TGATCA 1 cut(s) 456
BfaI CTAG 3 cut(s) 161, 225, 630
BisI GCNGC 3 cut(s) 130, 748, 974
BlsI GCNGC 3 cut(s) 131, 749, 975
Bme1390I CCNGG 1 cut(s) 777
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 2 cut(s) 91, 202
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 1 cut(s) 777
BmsI GCATC 1 cut(s) 950
BpiI GAAGAC 1 cut(s) 651
Bpu14I TTCGAA 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 519
BsaJI CCNNGG 7 cut(s) 109, 205, 339, 513, 596, 775, 980
BsaXI ACNNNNNCTCC 6 cut(s) 287, 317, 336, 366, 928, 958
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 387, 799
Bse1I ACTGG 2 cut(s) 618, 1037
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 7 cut(s) 109, 205, 339, 513, 596, 775, 980
BseGI GGATG 1 cut(s) 950
BseLI CCNNNNNNNGG 3 cut(s) 345, 387, 799
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 2 cut(s) 618, 1037
BseRI GAGGAG 3 cut(s) 57, 311, 314
BseXI GCAGC 3 cut(s) 141, 759, 985
BsgI GTGCAG 2 cut(s) 148, 337
Bsh1236I CGCG 3 cut(s) 170, 555, 982
Bsh1285I CGRYCG 1 cut(s) 980
BshFI GGCC 3 cut(s) 92, 204, 792
BsiEI CGRYCG 1 cut(s) 980
BsiHKCI CYCGRG 1 cut(s) 854
BslI CCNNNNNNNGG 3 cut(s) 345, 387, 799
BsmI GAATGC 1 cut(s) 918
BsnI GGCC 3 cut(s) 92, 204, 792
BsoBI CYCGRG 1 cut(s) 854
Bsp119I TTCGAA 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 810
Bsp143I GATC 4 cut(s) 376, 456, 541, 725
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 7 cut(s) 132, 170, 293, 449, 531, 980, 982
BspANI GGCC 3 cut(s) 92, 204, 792
BspCNI CTCAG 1 cut(s) 615
BspFNI CGCG 3 cut(s) 170, 555, 982
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 378
BspPI GGATC 3 cut(s) 371, 384, 536
BspT104I TTCGAA 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 274
BsrI ACTGG 2 cut(s) 618, 1037
BssECI CCNNGG 7 cut(s) 109, 205, 339, 513, 596, 775, 980
BssMI GATC 4 cut(s) 376, 456, 541, 725
BssNI GRCGYC 1 cut(s) 519
BssSI CACGAG 1 cut(s) 865
BssT1I CCWWGG 3 cut(s) 339, 513, 596
Bst2BI CACGAG 1 cut(s) 865
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 4 cut(s) 308, 703, 732, 771
BstACI GRCGYC 1 cut(s) 519
BstAPI GCANNNNNTGC 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 246
BstC8I GCNNGC 2 cut(s) 172, 745
BstDEI CTNAG 1 cut(s) 623
BstDSI CCRYGG 4 cut(s) 109, 205, 339, 980
BstF5I GGATG 1 cut(s) 950
BstFNI CGCG 3 cut(s) 170, 555, 982
BstHHI GCGC 2 cut(s) 555, 1017
BstKTI GATC 4 cut(s) 379, 459, 544, 728
BstMBI GATC 4 cut(s) 376, 456, 541, 725
BstMCI CGRYCG 1 cut(s) 980
BstMWI GCNNNNNNNGC 6 cut(s) 126, 176, 448, 859, 979, 1021
BstNI CCWGG 1 cut(s) 777
BstSCI CCNGG 1 cut(s) 775
BstUI CGCG 3 cut(s) 170, 555, 982
BstV1I GCAGC 3 cut(s) 141, 759, 985
BstV2I GAAGAC 1 cut(s) 651
BstX2I RGATCY 2 cut(s) 376, 541
BstXI CCANNNNNNTGG 1 cut(s) 116
BstYI RGATCY 2 cut(s) 376, 541
BsuRI GGCC 3 cut(s) 92, 204, 792
BtgI CCRYGG 4 cut(s) 109, 205, 339, 980
BtsCI GGATG 1 cut(s) 950
BtsIMutI CAGTG 3 cut(s) 611, 728, 1044
Cac8I GCNNGC 2 cut(s) 172, 745
CciI TCATGA 1 cut(s) 138
CfoI GCGC 2 cut(s) 555, 1017
Cfr13I GGNCC 2 cut(s) 91, 202
Cfr42I CCGCGG 1 cut(s) 983
Csp6I GTAC 4 cut(s) 304, 328, 506, 772
CspCI CAANNNNNGTGG 2 cut(s) 27, 62
CviAII CATG 8 cut(s) 121, 139, 340, 415, 634, 656, 686, 914
CviQI GTAC 4 cut(s) 304, 328, 506, 772
DdeI CTNAG 1 cut(s) 623
DpnI GATC 4 cut(s) 378, 458, 543, 727
DpnII GATC 4 cut(s) 376, 456, 541, 725
DrdI GACNNNNNNGTC 1 cut(s) 881
DseDI GACNNNNNNGTC 1 cut(s) 881
EciI GGCGGA 1 cut(s) 520
Eco130I CCWWGG 3 cut(s) 339, 513, 596
Eco24I GRGCYC 1 cut(s) 810
Eco32I GATATC 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 854
EcoO109I RGGNCCY 1 cut(s) 91
EcoRI GAATTC 2 cut(s) 34, 272
EcoRII CCWGG 1 cut(s) 775
EcoRV GATATC 1 cut(s) 426
EcoT14I CCWWGG 3 cut(s) 339, 513, 596
EcoT38I GRGCYC 1 cut(s) 810
ErhI CCWWGG 3 cut(s) 339, 513, 596
FaeI CATG 8 cut(s) 124, 142, 343, 418, 637, 659, 689, 917
FatI CATG 8 cut(s) 120, 138, 339, 414, 633, 655, 685, 913
FauI CCCGC 2 cut(s) 163, 456
FbaI TGATCA 1 cut(s) 456
Fnu4HI GCNGC 3 cut(s) 130, 748, 974
FokI GGATG 1 cut(s) 937
FriOI GRGCYC 1 cut(s) 810
Fsp4HI GCNGC 3 cut(s) 130, 748, 974
FspBI CTAG 3 cut(s) 161, 225, 630
GlaI GCGC 2 cut(s) 554, 1016
GluI GCNGC 3 cut(s) 130, 748, 974
HaeIII GGCC 3 cut(s) 92, 204, 792
HhaI GCGC 2 cut(s) 555, 1017
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 8 cut(s) 124, 142, 343, 418, 637, 659, 689, 917
Hin6I GCGC 2 cut(s) 553, 1015
HinP1I GCGC 2 cut(s) 553, 1015
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 4 cut(s) 154, 189, 335, 527
HphI GGTGA 2 cut(s) 466, 673
Hpy166II GTNNAC 2 cut(s) 25, 259
Hpy188I TCNGA 3 cut(s) 430, 473, 924
Hpy188III TCNNGA 8 cut(s) 17, 39, 139, 216, 238, 587, 801, 856
Hpy8I GTNNAC 2 cut(s) 25, 259
Hpy99I CGWCG 2 cut(s) 802, 887
HpyAV CCTTC 2 cut(s) 367, 544
HpyCH4III ACNGT 4 cut(s) 308, 703, 732, 771
HpyCH4IV ACGT 5 cut(s) 211, 519, 797, 819, 905
HpyCH4V TGCA 9 cut(s) 63, 129, 267, 318, 410, 655, 685, 836, 892
HpyF10VI GCNNNNNNNGC 6 cut(s) 126, 176, 448, 859, 979, 1021
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 5 cut(s) 211, 519, 797, 819, 905
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 8 cut(s) 124, 142, 343, 418, 637, 659, 689, 917
HspAI GCGC 2 cut(s) 553, 1015
Ksp22I TGATCA 1 cut(s) 456
KspI CCGCGG 1 cut(s) 983
Kzo9I GATC 4 cut(s) 376, 456, 541, 725
LmnI GCTCC 2 cut(s) 49, 567
LpnPI CCDG 9 cut(s) 371, 437, 558, 599, 726, 757, 762, 789, 968
Lsp1109I GCAGC 3 cut(s) 141, 759, 985
LweI GCATC 1 cut(s) 950
MaeI CTAG 3 cut(s) 161, 225, 630
MaeII ACGT 5 cut(s) 211, 519, 797, 819, 905
MaeIII GTNAC 3 cut(s) 230, 308, 703
MalI GATC 4 cut(s) 378, 458, 543, 727
MboI GATC 4 cut(s) 376, 456, 541, 725
MboII GAAGA 4 cut(s) 268, 511, 656, 667
MflI RGATCY 2 cut(s) 376, 541
MhlI GDGCHC 1 cut(s) 810
MluCI AATT 3 cut(s) 34, 272, 285
MseI TTAA 3 cut(s) 467, 603, 909
MslI CAYNNNNRTG 3 cut(s) 114, 415, 867
MspA1I CMGCKG 3 cut(s) 132, 451, 982
MspR9I CCNGG 1 cut(s) 777
Mva1269I GAATGC 1 cut(s) 918
MvaI CCWGG 1 cut(s) 777
MvnI CGCG 3 cut(s) 170, 555, 982
MwoI GCNNNNNNNGC 6 cut(s) 126, 176, 448, 859, 979, 1021
NcoI CCATGG 1 cut(s) 339
NdeII GATC 4 cut(s) 376, 456, 541, 725
NlaIII CATG 8 cut(s) 124, 142, 343, 418, 637, 659, 689, 917
NlaIV GGNNCC 1 cut(s) 378
NspV TTCGAA 1 cut(s) 246
OliI CACNNNNGTG 2 cut(s) 415, 867
PaeR7I CTCGAG 1 cut(s) 854
PagI TCATGA 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 825
PctI GAATGC 1 cut(s) 918
PfeI GAWTC 4 cut(s) 154, 189, 335, 527
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 3 cut(s) 131, 749, 975
PshBI ATTAAT 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 775
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 2 cut(s) 91, 202
PsuI RGATCY 2 cut(s) 376, 541
RsaI GTAC 4 cut(s) 305, 329, 507, 773
RsaNI GTAC 4 cut(s) 304, 328, 506, 772
RseI CAYNNNNRTG 3 cut(s) 114, 415, 867
SacII CCGCGG 1 cut(s) 983
SaqAI TTAA 3 cut(s) 467, 603, 909
SatI GCNGC 3 cut(s) 130, 748, 974
Sau3AI GATC 4 cut(s) 376, 456, 541, 725
Sau96I GGNCC 2 cut(s) 91, 202
ScrFI CCNGG 1 cut(s) 777
SduI GDGCHC 1 cut(s) 810
SfaNI GCATC 1 cut(s) 950
Sfr274I CTCGAG 1 cut(s) 854
Sfr303I CCGCGG 1 cut(s) 983
SfuI TTCGAA 1 cut(s) 246
SgrBI CCGCGG 1 cut(s) 983
SlaI CTCGAG 1 cut(s) 854
SmiMI CAYNNNNRTG 3 cut(s) 114, 415, 867
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 3 cut(s) 34, 272, 285
SsiI CCGC 7 cut(s) 132, 170, 293, 449, 531, 980, 982
SspMI CTAG 3 cut(s) 161, 225, 630
StyD4I CCNGG 1 cut(s) 775
StyI CCWWGG 3 cut(s) 339, 513, 596
TaaI ACNGT 4 cut(s) 308, 703, 732, 771
TaiI ACGT 5 cut(s) 214, 522, 800, 822, 908
TaqI TCGA 4 cut(s) 192, 215, 246, 855
TasI AATT 3 cut(s) 34, 272, 285
TfiI GAWTC 4 cut(s) 154, 189, 335, 527
Tru1I TTAA 3 cut(s) 467, 603, 909
Tru9I TTAA 3 cut(s) 467, 603, 909
TscAI CASTG 3 cut(s) 618, 735, 1044
TseI GCWGC 3 cut(s) 129, 747, 973
TspDTI ATGAA 4 cut(s) 69, 127, 285, 944
TspGWI ACGGA 3 cut(s) 222, 345, 871
TspRI CASTG 3 cut(s) 618, 735, 1044
Van91I CCANNNNNTGG 1 cut(s) 387
VspI ATTAAT 1 cut(s) 467
XapI RAATTY 2 cut(s) 34, 272
XhoI CTCGAG 1 cut(s) 854
XspI CTAG 3 cut(s) 161, 225, 630
ZraI GACGTC 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.