RchiOBHm_Chr6g0294021

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006691
Physical Location & Seq
Reverse (-)
56510693 .. 56514438
3746 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26386

Sequence Viewer

Length: 1032 bp
ATGGATGGTGAATATCAAGAAGCTTACATAAGGAATTCCAGAGGAGTGCAGCTTTTCACTTGCAGATGGTTGCCTTTCTCTTCCCCAAAGGCCCTTGTTTTCCTTTGCCACGGTTACGGCATGGAGTGCAGCGGTTTCATGAAAGAATGCGGGGTCCGGCTAGCTAATGCCGGGTATGCGGTGTTCGGGATCGATTACGAGGGCCACGGACGTTCTCGGGGGGCAAGGTGTTACATTAAGAAGTTCGAAAACATCGTTAACGATTGCAATGACTTCTTCAAGTCGATTTGTGCGGAGGAAGAGTACAGGGACAAGTGTAGGTTCTTGTATGGGGAATCCATGGGAGGGGCAGTGGCCTTATTGCTTCATAAGAAGGATCCTAAATTTTGGAATGGTGCTGTTCTTGTTGCACCAATGTGTAAGATATCAGAGAAAGTAAAGCCGCATCCACTGGTTGTGAATGTATTGACTAGAGTGGAGGAGATTATACCGAAATGGAAGATTGTACCCACCAAAGACGTCATTGATTCAGCCTTCAAGGACCCTGTAAAGCGCGAAGTGATAAGAAGCAACAAGCTTATCTACCAAGACAAGCCGAGGCTGAAAACAGCTTTGGAAATGCTTAGAACTAGCATGAGCCTTGAAGACAGTTTACATGAGGTGAAACTGCCTTTCTTTGTTTTACACGGGGAGGCAGATACAGTTACGGACCCTGAAGTAAGCAAGTGTCTGTACGAAAAAGCCAGCAGCCCAGACAAGACCATAAAATTGTACCCTGGAATGTGGCATGCCTTAACATCAGGAGAGCCTGATGGGAACGTCGAAATCGTGTTTGCAGACATCACAGTTTGGCTTGAGAAGCACACTACAGACAGCAATGTCATGGTTGTGCAGCCAATTCAAGCATTTAATGACGGCATTGTGAAGTTGAACACCACAGCATCATCACCTGAAACCATGAACAAGCGGCAGAAAGGGAGCTATTTTTGTGGGTTAAAGGGACGAAGATTACAACATCACTCAGCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 ko05225 map00561 map01100 map04714 map04723 map04923 map05225
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

343

Amino Acids

38.83

Weight (kDa)

8.52

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 28 - 270 3.5e-65 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 32 - 143 2.5e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 33 - 266 8.3e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 878
AatII GACGTC 1 cut(s) 522
AccII CGCG 1 cut(s) 555
AciI CCGC 6 cut(s) 132, 150, 179, 293, 443, 967
AclWI GGATC 3 cut(s) 197, 371, 384
AcsI RAATTY 2 cut(s) 34, 383
AcuI CTGAAG 1 cut(s) 735
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 4 cut(s) 305, 507, 734, 773
AfiI CCNNNNNNNGG 1 cut(s) 345
AgsI TTSAA 5 cut(s) 280, 538, 644, 902, 931
AjnI CCWGG 1 cut(s) 775
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
AleI CACNNNNGTG 1 cut(s) 415
AluBI AGCT 6 cut(s) 23, 52, 164, 577, 611, 981
AluI AGCT 6 cut(s) 23, 52, 164, 577, 611, 981
AlwI GGATC 3 cut(s) 197, 371, 384
Ama87I CYCGRG 1 cut(s) 216
AoxI GGCC 3 cut(s) 90, 202, 354
ApeKI GCWGC 4 cut(s) 49, 129, 747, 892
ApoI RAATTY 2 cut(s) 34, 383
AspLEI GCGC 1 cut(s) 555
AspS9I GGNCC 5 cut(s) 91, 154, 202, 541, 709
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 3 cut(s) 20, 673, 939
AsuII TTCGAA 1 cut(s) 246
AsuNHI GCTAGC 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 3 cut(s) 154, 541, 709
BamHI GGATCC 1 cut(s) 376
BarI GAAGNNNNNNTAC 2 cut(s) 636, 668
BbsI GAAGAC 1 cut(s) 651
BbvI GCAGC 4 cut(s) 61, 141, 759, 904
BccI CCATC 2 cut(s) 60, 806
BceAI ACGGC 2 cut(s) 133, 931
BciT130I CCWGG 1 cut(s) 777
BcnI CCSGG 1 cut(s) 172
BfaI CTAG 3 cut(s) 161, 471, 630
BfmI CTRYAG 1 cut(s) 867
BisI GCNGC 6 cut(s) 50, 130, 443, 748, 893, 968
BlsI GCNGC 6 cut(s) 51, 131, 444, 749, 894, 969
Bme1390I CCNGG 2 cut(s) 172, 777
Bme18I GGWCC 3 cut(s) 154, 541, 709
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 5 cut(s) 91, 154, 202, 541, 709
BmiI GGNNCC 4 cut(s) 155, 378, 543, 711
BmrFI CCNGG 2 cut(s) 172, 777
BmsI GCATC 2 cut(s) 454, 950
BmtI GCTAGC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 651
Bpu14I TTCGAA 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 1 cut(s) 172
Bsa29I ATCGAT 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 519
BsaJI CCNNGG 5 cut(s) 109, 205, 339, 596, 775
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 336, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 345
Bse1I ACTGG 1 cut(s) 456
Bse3DI GCAATG 3 cut(s) 274, 883, 1032
BseBI CCWGG 1 cut(s) 777
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 5 cut(s) 109, 205, 339, 596, 775
BseGI GGATG 2 cut(s) 10, 445
BseLI CCNNNNNNNGG 1 cut(s) 345
BseMI GCAATG 3 cut(s) 274, 883, 1032
BseNI ACTGG 1 cut(s) 456
BseRI GAGGAG 2 cut(s) 57, 494
BseXI GCAGC 4 cut(s) 61, 141, 759, 904
BsgI GTGCAG 3 cut(s) 68, 148, 911
Bsh1236I CGCG 1 cut(s) 555
BshFI GGCC 3 cut(s) 92, 204, 356
BshVI ATCGAT 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 2 cut(s) 157, 171
BslFI GGGAC 2 cut(s) 323, 1014
BslI CCNNNNNNNGG 1 cut(s) 345
BsmFI GGGAC 2 cut(s) 323, 1014
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 3 cut(s) 92, 204, 356
BsoBI CYCGRG 1 cut(s) 216
Bsp119I TTCGAA 1 cut(s) 246
Bsp143I GATC 2 cut(s) 189, 376
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 6 cut(s) 132, 150, 179, 293, 443, 967
BspANI GGCC 3 cut(s) 92, 204, 356
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 1 cut(s) 555
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 4 cut(s) 155, 378, 543, 711
BspOI GCTAGC 1 cut(s) 164
BspPI GGATC 3 cut(s) 197, 371, 384
BspT104I TTCGAA 1 cut(s) 246
BsrDI GCAATG 3 cut(s) 274, 883, 1032
BsrI ACTGG 1 cut(s) 456
BssECI CCNNGG 5 cut(s) 109, 205, 339, 596, 775
BssMI GATC 2 cut(s) 189, 376
BssNI GRCGYC 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 4 cut(s) 113, 650, 703, 847
Bst6I CTCTTC 2 cut(s) 85, 294
BstACI GRCGYC 1 cut(s) 519
BstAPI GCANNNNNTGC 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 246
BstC8I GCNNGC 3 cut(s) 162, 745, 789
BstDEI CTNAG 2 cut(s) 623, 1021
BstDSI CCRYGG 3 cut(s) 109, 205, 339
BstF5I GGATG 2 cut(s) 10, 445
BstFNI CGCG 1 cut(s) 555
BstHHI GCGC 1 cut(s) 555
BstKTI GATC 2 cut(s) 192, 379
BstMBI GATC 2 cut(s) 189, 376
BstMWI GCNNNNNNNGC 3 cut(s) 126, 176, 859
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 1 cut(s) 791
BstSCI CCNGG 2 cut(s) 170, 775
BstSFI CTRYAG 1 cut(s) 867
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 4 cut(s) 61, 141, 759, 904
BstV2I GAAGAC 1 cut(s) 651
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 3 cut(s) 92, 204, 356
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 3 cut(s) 109, 205, 339
BtsCI GGATG 2 cut(s) 10, 445
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 357, 449
Cac8I GCNNGC 3 cut(s) 162, 745, 789
CciI TCATGA 1 cut(s) 138
CfoI GCGC 1 cut(s) 555
Cfr13I GGNCC 5 cut(s) 91, 154, 202, 541, 709
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 4 cut(s) 304, 506, 733, 772
CviAII CATG 8 cut(s) 121, 139, 340, 634, 656, 788, 883, 958
CviQI GTAC 4 cut(s) 304, 506, 733, 772
DdeI CTNAG 2 cut(s) 623, 1021
DpnI GATC 2 cut(s) 191, 378
DpnII GATC 2 cut(s) 189, 376
DrdI GACNNNNNNGTC 1 cut(s) 878
DseDI GACNNNNNNGTC 1 cut(s) 878
Eam1104I CTCTTC 2 cut(s) 85, 294
EarI CTCTTC 2 cut(s) 85, 294
Eco130I CCWWGG 1 cut(s) 339
Eco32I GATATC 1 cut(s) 426
Eco47I GGWCC 3 cut(s) 154, 541, 709
Eco57I CTGAAG 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 91, 541
EcoRI GAATTC 1 cut(s) 34
EcoRII CCWGG 1 cut(s) 775
EcoRV GATATC 1 cut(s) 426
EcoT14I CCWWGG 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 8 cut(s) 124, 142, 343, 637, 659, 791, 886, 961
FaqI GGGAC 2 cut(s) 323, 1014
FatI CATG 8 cut(s) 120, 138, 339, 633, 655, 787, 882, 957
FauI CCCGC 1 cut(s) 143
Fnu4HI GCNGC 6 cut(s) 50, 130, 443, 748, 893, 968
FokI GGATG 2 cut(s) 17, 432
Fsp4HI GCNGC 6 cut(s) 50, 130, 443, 748, 893, 968
FspBI CTAG 3 cut(s) 161, 471, 630
GlaI GCGC 1 cut(s) 554
GluI GCNGC 6 cut(s) 50, 130, 443, 748, 893, 968
HaeIII GGCC 3 cut(s) 92, 204, 356
HapII CCGG 2 cut(s) 157, 171
HhaI GCGC 1 cut(s) 555
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 8 cut(s) 124, 142, 343, 637, 659, 791, 886, 961
Hin6I GCGC 1 cut(s) 553
HinP1I GCGC 1 cut(s) 553
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 2 cut(s) 21, 575
HinfI GANTC 2 cut(s) 335, 527
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 2 cut(s) 157, 171
HphI GGTGA 3 cut(s) 20, 673, 939
Hpy166II GTNNAC 2 cut(s) 259, 653
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 5 cut(s) 17, 39, 139, 187, 801
Hpy8I GTNNAC 2 cut(s) 259, 653
Hpy99I CGWCG 1 cut(s) 824
HpyAV CCTTC 2 cut(s) 367, 544
HpyCH4III ACNGT 4 cut(s) 113, 650, 703, 847
HpyCH4IV ACGT 3 cut(s) 211, 519, 819
HpyCH4V TGCA 7 cut(s) 49, 63, 129, 267, 410, 836, 892
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 176, 859
HpyF3I CTNAG 2 cut(s) 623, 1021
HpySE526I ACGT 3 cut(s) 211, 519, 819
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 8 cut(s) 124, 142, 343, 637, 659, 791, 886, 961
HspAI GCGC 1 cut(s) 553
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 2 cut(s) 189, 376
LmnI GCTCC 1 cut(s) 978
Lsp1109I GCAGC 4 cut(s) 61, 141, 759, 904
LweI GCATC 2 cut(s) 454, 950
MaeI CTAG 3 cut(s) 161, 471, 630
MaeII ACGT 3 cut(s) 211, 519, 819
MaeIII GTNAC 3 cut(s) 113, 230, 703
MalI GATC 2 cut(s) 191, 378
MboI GATC 2 cut(s) 189, 376
MboII GAAGA 6 cut(s) 72, 268, 311, 511, 656, 1017
MflI RGATCY 1 cut(s) 376
MluCI AATT 4 cut(s) 34, 383, 767, 897
MnlI CCTC 8 cut(s) 35, 193, 289, 338, 472, 591, 652, 685
MseI TTAA 5 cut(s) 237, 258, 794, 909, 995
MslI CAYNNNNRTG 2 cut(s) 415, 887
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 2 cut(s) 157, 171
MspR9I CCNGG 2 cut(s) 172, 777
Mva1269I GAATGC 1 cut(s) 152
MvaI CCWGG 1 cut(s) 777
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 3 cut(s) 126, 176, 859
NciI CCSGG 1 cut(s) 172
NcoI CCATGG 1 cut(s) 339
NdeII GATC 2 cut(s) 189, 376
NheI GCTAGC 1 cut(s) 160
NlaIII CATG 8 cut(s) 124, 142, 343, 637, 659, 791, 886, 961
NlaIV GGNNCC 4 cut(s) 155, 378, 543, 711
NmeAIII GCCGAG 1 cut(s) 621
NspI RCATGY 1 cut(s) 791
NspV TTCGAA 1 cut(s) 246
OliI CACNNNNGTG 1 cut(s) 415
PaeI GCATGC 1 cut(s) 791
PagI TCATGA 1 cut(s) 138
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 825
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 2 cut(s) 335, 527
PkrI GCNGC 6 cut(s) 51, 131, 444, 749, 894, 969
PpuMI RGGWCCY 1 cut(s) 541
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 775
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 4 cut(s) 155, 378, 543, 711
PspPI GGNCC 5 cut(s) 91, 154, 202, 541, 709
PspPPI RGGWCCY 1 cut(s) 541
PsrI GAACNNNNNNTAC 2 cut(s) 167, 199
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 4 cut(s) 305, 507, 734, 773
RsaNI GTAC 4 cut(s) 304, 506, 733, 772
RseI CAYNNNNRTG 2 cut(s) 415, 887
SaqAI TTAA 5 cut(s) 237, 258, 794, 909, 995
SatI GCNGC 6 cut(s) 50, 130, 443, 748, 893, 968
Sau3AI GATC 2 cut(s) 189, 376
Sau96I GGNCC 5 cut(s) 91, 154, 202, 541, 709
ScrFI CCNGG 2 cut(s) 172, 777
SfaNI GCATC 2 cut(s) 454, 950
SfcI CTRYAG 1 cut(s) 867
SfuI TTCGAA 1 cut(s) 246
SinI GGWCC 3 cut(s) 154, 541, 709
SmiMI CAYNNNNRTG 2 cut(s) 415, 887
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
SphI GCATGC 1 cut(s) 791
Sse9I AATT 4 cut(s) 34, 383, 767, 897
SsiI CCGC 6 cut(s) 132, 150, 179, 293, 443, 967
SspMI CTAG 3 cut(s) 161, 471, 630
StyD4I CCNGG 2 cut(s) 170, 775
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 4 cut(s) 113, 650, 703, 847
TaiI ACGT 3 cut(s) 214, 522, 822
TaqI TCGA 4 cut(s) 192, 246, 284, 822
TasI AATT 4 cut(s) 34, 383, 767, 897
TatI WGTACW 1 cut(s) 303
TauI GCSGC 2 cut(s) 445, 970
TfiI GAWTC 2 cut(s) 335, 527
Tru1I TTAA 5 cut(s) 237, 258, 794, 909, 995
Tru9I TTAA 5 cut(s) 237, 258, 794, 909, 995
TscAI CASTG 2 cut(s) 357, 456
TseI GCWGC 4 cut(s) 49, 129, 747, 892
TspDTI ATGAA 4 cut(s) 127, 155, 356, 974
TspGWI ACGGA 2 cut(s) 222, 722
TspRI CASTG 2 cut(s) 357, 456
VpaK11BI GGWCC 3 cut(s) 154, 541, 709
XapI RAATTY 2 cut(s) 34, 383
XceI RCATGY 1 cut(s) 791
XspI CTAG 3 cut(s) 161, 471, 630
ZraI GACGTC 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.