MD11G1309800.v1.1

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006691
Physical Location & Seq
Forward (+)
42315614 .. 42319994
4381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1309800.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGAGGGAGAGTATCAAGAAGTTTACATAAGGAATTCAAGAGGAGTGCAGCTTTTTACTTGCAGATGGTTGCCCTTTTCCTCTCCAAAGGCCCTTGTGTTCCTTTGCCATGGGTATGGCATGGAGTGCAGCGGTTTCATGAGAGAGTGTGGAATCCGCCTAGCTAACGCGGGATATGCCGTGTTCGGAATTGACTACGAGGGCCACGGACGTTCGAGAGGGGCCAGATGTTACATCAAGAAGTTCAAAAACATAGTCAACGATTGCGATGAATTTTTCAAGTCGATTTGTGCCGAGGAAGAGTACCGAGACAAGTGTAGGTTTTTGTATGGAGAATCCATGGGAGGGGCAGTTTCTCTACTGCTTCACAAGAAGGATCCCACATTTTGGAATGGAGCTGTTCTTGTTGCACCCATGTGTAAGATATCAGAAAAGGTCAAGCCACACCCGTTGGTGATCAATGTATTAACCAGAGTGGAGGAGATTATTCCCAAATGGAAGATAGTACCGACCAAAGACGTCATTGACTCCGCCTTCAAAGACCCTGTAAAGCGTGAAGAGATAAGGAGCAACAAACTGATATATCAAGACAAGCCGAGGCTGAAAACAGCACTGGAAATGCTGAGAACTAGCATGAGCCTTGAAGACACTTTGCATGAGGTGAGACTACCATTCTTTGTGTTGCATGGGGAGGCAGATACTGTTACAGACCCAGAAGTAAGCAGAGCACTGTATGAGAAAGCCAGCAGCACAGACAAGACCATAAAATTGTACCCCGGAATGTGGCATGGTCTAACGTCGGGAGAGCCAGATGGGAACGTTGAAATCGTCTTTGCAGACATCATAACTTGGCTCGAGAAGCACACGAGTGACGACAACTCCGTCGTGGTGCAACCAATCCACACGTTTAGGAATGCCATTCTGAAAATGAACGGCACGGCATCACCACCACCCCCGCCACCAATAAACAAGCAGCGACCGCGGACGCCATCGTATTTTTGCGGGTTGAAGGGGCGCAGATTGCTACATCACTCGGCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 ko05225 map00561 map01100 map04714 map04723 map04923 map05225
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

348

Amino Acids

39.46

Weight (kDa)

8.69

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 28 - 270 9.3e-66 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 32 - 147 6.7e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 33 - 264 2.1e-08 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 881
AatII GACGTC 1 cut(s) 522
AccB7I CCANNNNNTGG 1 cut(s) 387
AccII CGCG 2 cut(s) 170, 982
AciI CCGC 8 cut(s) 132, 157, 170, 531, 956, 980, 982, 1002
AclI AACGTT 1 cut(s) 819
AclWI GGATC 2 cut(s) 371, 384
AcsI RAATTY 2 cut(s) 34, 272
AcyI GRCGYC 2 cut(s) 519, 986
AfaI GTAC 3 cut(s) 305, 507, 773
AfiI CCNNNNNNNGG 3 cut(s) 345, 387, 783
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 7 cut(s) 39, 247, 280, 538, 644, 824, 1009
AleI CACNNNNGTG 2 cut(s) 415, 867
AluBI AGCT 3 cut(s) 52, 164, 398
AluI AGCT 3 cut(s) 52, 164, 398
Alw21I GWGCWC 1 cut(s) 730
Alw26I GTCTC 2 cut(s) 303, 658
AlwI GGATC 2 cut(s) 371, 384
AlwNI CAGNNNCTG 1 cut(s) 701
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 3 cut(s) 90, 202, 222
ApeKI GCWGC 4 cut(s) 49, 129, 747, 973
ApoI RAATTY 2 cut(s) 34, 272
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AspLEI GCGC 1 cut(s) 1017
AspS9I GGNCC 3 cut(s) 91, 202, 222
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 3 cut(s) 466, 673, 936
AvaI CYCGRG 1 cut(s) 854
BamHI GGATCC 1 cut(s) 376
BauI CACGAG 1 cut(s) 865
BbsI GAAGAC 1 cut(s) 651
Bbv12I GWGCWC 1 cut(s) 730
BbvI GCAGC 4 cut(s) 61, 141, 759, 985
BccI CCATC 3 cut(s) 60, 806, 997
BceAI ACGGC 3 cut(s) 164, 949, 954
BclI TGATCA 1 cut(s) 456
BcnI CCSGG 1 cut(s) 777
BcoDI GTCTC 2 cut(s) 303, 658
BfaI CTAG 2 cut(s) 161, 630
BisI GCNGC 4 cut(s) 50, 130, 748, 974
BlsI GCNGC 4 cut(s) 51, 131, 749, 975
Bme1390I CCNGG 1 cut(s) 777
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 3 cut(s) 91, 202, 222
BmiI GGNNCC 2 cut(s) 223, 378
BmrFI CCNGG 1 cut(s) 777
BmsI GCATC 1 cut(s) 950
BpiI GAAGAC 1 cut(s) 651
BpuMI CCSGG 1 cut(s) 777
BsaHI GRCGYC 2 cut(s) 519, 986
BsaJI CCNNGG 7 cut(s) 109, 205, 294, 339, 596, 775, 980
BsaXI ACNNNNNCTCC 5 cut(s) 26, 336, 366, 863, 893
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 387, 783
Bse1I ACTGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 1044
BseDI CCNNGG 7 cut(s) 109, 205, 294, 339, 596, 775, 980
BseLI CCNNNNNNNGG 3 cut(s) 345, 387, 783
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 1 cut(s) 618
BseRI GAGGAG 2 cut(s) 57, 494
BseXI GCAGC 4 cut(s) 61, 141, 759, 985
BsgI GTGCAG 2 cut(s) 68, 148
Bsh1236I CGCG 2 cut(s) 170, 982
Bsh1285I CGRYCG 1 cut(s) 980
BshFI GGCC 3 cut(s) 92, 204, 224
BsiEI CGRYCG 1 cut(s) 980
BsiHKAI GWGCWC 1 cut(s) 730
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 1 cut(s) 777
BslI CCNNNNNNNGG 3 cut(s) 345, 387, 783
BsmAI GTCTC 2 cut(s) 303, 658
BsmI GAATGC 1 cut(s) 919
BsnI GGCC 3 cut(s) 92, 204, 224
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 1 cut(s) 730
Bsp143I GATC 2 cut(s) 376, 456
Bsp19I CCATGG 2 cut(s) 109, 339
BspACI CCGC 8 cut(s) 132, 157, 170, 531, 956, 980, 982, 1002
BspANI GGCC 3 cut(s) 92, 204, 224
BspCNI CTCAG 1 cut(s) 615
BspFNI CGCG 2 cut(s) 170, 982
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 223, 378
BspPI GGATC 2 cut(s) 371, 384
BsrDI GCAATG 1 cut(s) 1044
BsrI ACTGG 1 cut(s) 618
BssECI CCNNGG 7 cut(s) 109, 205, 294, 339, 596, 775, 980
BssMI GATC 2 cut(s) 376, 456
BssNI GRCGYC 2 cut(s) 519, 986
BssSI CACGAG 1 cut(s) 865
BssT1I CCWWGG 2 cut(s) 109, 339
Bst2BI CACGAG 1 cut(s) 865
Bst4CI ACNGT 2 cut(s) 703, 732
Bst6I CTCTTC 2 cut(s) 294, 552
BstACI GRCGYC 2 cut(s) 519, 986
BstAPI GCANNNNNTGC 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 745
BstDEI CTNAG 1 cut(s) 623
BstDSI CCRYGG 4 cut(s) 109, 205, 339, 980
BstFNI CGCG 2 cut(s) 170, 982
BstHHI GCGC 1 cut(s) 1017
BstKTI GATC 2 cut(s) 379, 459
BstMAI GTCTC 2 cut(s) 303, 658
BstMBI GATC 2 cut(s) 376, 456
BstMCI CGRYCG 1 cut(s) 980
BstMWI GCNNNNNNNGC 5 cut(s) 126, 176, 859, 979, 1021
BstSCI CCNGG 1 cut(s) 775
BstUI CGCG 2 cut(s) 170, 982
BstV1I GCAGC 4 cut(s) 61, 141, 759, 985
BstV2I GAAGAC 1 cut(s) 651
BstX2I RGATCY 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 116
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 3 cut(s) 92, 204, 224
BtgI CCRYGG 4 cut(s) 109, 205, 339, 980
BtgZI GCGATG 1 cut(s) 282
BtsIMutI CAGTG 2 cut(s) 611, 728
Cac8I GCNNGC 1 cut(s) 745
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 138
CfoI GCGC 1 cut(s) 1017
Cfr13I GGNCC 3 cut(s) 91, 202, 222
Cfr42I CCGCGG 1 cut(s) 983
CseI GACGC 1 cut(s) 994
Csp6I GTAC 3 cut(s) 304, 506, 772
CspCI CAANNNNNGTGG 2 cut(s) 432, 467
CviAII CATG 9 cut(s) 110, 121, 139, 340, 415, 634, 656, 686, 788
CviQI GTAC 3 cut(s) 304, 506, 772
DdeI CTNAG 1 cut(s) 623
DpnI GATC 2 cut(s) 378, 458
DpnII GATC 2 cut(s) 376, 456
DrdI GACNNNNNNGTC 1 cut(s) 881
DseDI GACNNNNNNGTC 1 cut(s) 881
Eam1104I CTCTTC 2 cut(s) 294, 552
EarI CTCTTC 2 cut(s) 294, 552
EciI GGCGGA 2 cut(s) 146, 520
Eco130I CCWWGG 2 cut(s) 109, 339
Eco32I GATATC 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 854
EcoO109I RGGNCCY 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 34
EcoRV GATATC 1 cut(s) 426
EcoT14I CCWWGG 2 cut(s) 109, 339
ErhI CCWWGG 2 cut(s) 109, 339
FaeI CATG 9 cut(s) 113, 124, 142, 343, 418, 637, 659, 689, 791
FatI CATG 9 cut(s) 109, 120, 138, 339, 414, 633, 655, 685, 787
FauI CCCGC 3 cut(s) 163, 963, 995
FbaI TGATCA 1 cut(s) 456
Fnu4HI GCNGC 4 cut(s) 50, 130, 748, 974
Fsp4HI GCNGC 4 cut(s) 50, 130, 748, 974
FspBI CTAG 2 cut(s) 161, 630
GlaI GCGC 1 cut(s) 1016
GluI GCNGC 4 cut(s) 50, 130, 748, 974
HaeIII GGCC 3 cut(s) 92, 204, 224
HapII CCGG 1 cut(s) 777
HgaI GACGC 1 cut(s) 994
HhaI GCGC 1 cut(s) 1017
Hin1I GRCGYC 2 cut(s) 519, 986
Hin1II CATG 9 cut(s) 113, 124, 142, 343, 418, 637, 659, 689, 791
Hin6I GCGC 1 cut(s) 1015
HinP1I GCGC 1 cut(s) 1015
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 3 cut(s) 153, 335, 527
HpaII CCGG 1 cut(s) 777
HphI GGTGA 3 cut(s) 466, 673, 936
Hpy166II GTNNAC 2 cut(s) 25, 259
Hpy188I TCNGA 3 cut(s) 188, 430, 924
Hpy188III TCNNGA 8 cut(s) 17, 39, 139, 216, 238, 587, 801, 856
Hpy8I GTNNAC 2 cut(s) 25, 259
Hpy99I CGWCG 2 cut(s) 802, 887
HpyAV CCTTC 3 cut(s) 367, 544, 1003
HpyCH4III ACNGT 2 cut(s) 703, 732
HpyCH4IV ACGT 5 cut(s) 211, 519, 797, 819, 905
HpyCH4V TGCA 8 cut(s) 49, 63, 129, 410, 655, 685, 836, 892
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 176, 859, 979, 1021
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 5 cut(s) 211, 519, 797, 819, 905
Hsp92I GRCGYC 2 cut(s) 519, 986
Hsp92II CATG 9 cut(s) 113, 124, 142, 343, 418, 637, 659, 689, 791
HspAI GCGC 1 cut(s) 1015
Ksp22I TGATCA 1 cut(s) 456
KspI CCGCGG 1 cut(s) 983
Kzo9I GATC 2 cut(s) 376, 456
LmnI GCTCC 2 cut(s) 395, 567
LpnPI CCDG 8 cut(s) 238, 484, 558, 599, 726, 757, 790, 822
Lsp1109I GCAGC 4 cut(s) 61, 141, 759, 985
LweI GCATC 1 cut(s) 950
MaeI CTAG 2 cut(s) 161, 630
MaeII ACGT 5 cut(s) 211, 519, 797, 819, 905
MaeIII GTNAC 3 cut(s) 230, 703, 869
MalI GATC 2 cut(s) 378, 458
MboI GATC 2 cut(s) 376, 456
MboII GAAGA 4 cut(s) 311, 511, 569, 656
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 730
MluCI AATT 4 cut(s) 34, 189, 272, 767
MlyI GAGTC 1 cut(s) 521
MseI TTAA 1 cut(s) 467
MslI CAYNNNNRTG 3 cut(s) 114, 415, 867
MspA1I CMGCKG 2 cut(s) 132, 982
MspI CCGG 1 cut(s) 777
MspR9I CCNGG 1 cut(s) 777
Mva1269I GAATGC 1 cut(s) 919
MvnI CGCG 2 cut(s) 170, 982
MwoI GCNNNNNNNGC 5 cut(s) 126, 176, 859, 979, 1021
NciI CCSGG 1 cut(s) 777
NcoI CCATGG 2 cut(s) 109, 339
NdeII GATC 2 cut(s) 376, 456
NlaIII CATG 9 cut(s) 113, 124, 142, 343, 418, 637, 659, 689, 791
NlaIV GGNNCC 2 cut(s) 223, 378
NmeAIII GCCGAG 3 cut(s) 319, 621, 1013
NmuCI GTSAC 1 cut(s) 869
OliI CACNNNNGTG 2 cut(s) 415, 867
PaeR7I CTCGAG 1 cut(s) 854
PagI TCATGA 1 cut(s) 138
PcsI WCGNNNNNNNCGW 2 cut(s) 825, 879
PctI GAATGC 1 cut(s) 919
PfeI GAWTC 2 cut(s) 153, 335
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 4 cut(s) 51, 131, 749, 975
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Psp1406I AACGTT 1 cut(s) 819
PspN4I GGNNCC 2 cut(s) 223, 378
PspPI GGNCC 3 cut(s) 91, 202, 222
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 3 cut(s) 305, 507, 773
RsaNI GTAC 3 cut(s) 304, 506, 772
RseI CAYNNNNRTG 3 cut(s) 114, 415, 867
SacII CCGCGG 1 cut(s) 983
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 4 cut(s) 50, 130, 748, 974
Sau3AI GATC 2 cut(s) 376, 456
Sau96I GGNCC 3 cut(s) 91, 202, 222
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 1 cut(s) 777
SduI GDGCHC 1 cut(s) 730
SfaNI GCATC 1 cut(s) 950
Sfr274I CTCGAG 1 cut(s) 854
Sfr303I CCGCGG 1 cut(s) 983
SgrBI CCGCGG 1 cut(s) 983
SlaI CTCGAG 1 cut(s) 854
SmiMI CAYNNNNRTG 3 cut(s) 114, 415, 867
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 4 cut(s) 34, 189, 272, 767
SsiI CCGC 8 cut(s) 132, 157, 170, 531, 956, 980, 982, 1002
SspMI CTAG 2 cut(s) 161, 630
StyD4I CCNGG 1 cut(s) 775
StyI CCWWGG 2 cut(s) 109, 339
TaaI ACNGT 2 cut(s) 703, 732
TaiI ACGT 5 cut(s) 214, 522, 800, 822, 908
TaqI TCGA 3 cut(s) 215, 284, 855
TasI AATT 4 cut(s) 34, 189, 272, 767
TfiI GAWTC 2 cut(s) 153, 335
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TscAI CASTG 2 cut(s) 618, 735
TseFI GTSAC 1 cut(s) 869
TseI GCWGC 4 cut(s) 49, 129, 747, 973
Tsp45I GTSAC 1 cut(s) 869
TspDTI ATGAA 3 cut(s) 127, 285, 944
TspGWI ACGGA 2 cut(s) 222, 871
TspRI CASTG 2 cut(s) 618, 735
Van91I CCANNNNNTGG 1 cut(s) 387
XapI RAATTY 2 cut(s) 34, 272
XhoI CTCGAG 1 cut(s) 854
XspI CTAG 2 cut(s) 161, 630
ZraI GACGTC 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.