AT4G00060

Nucleotidyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003190
Physical Location & Seq
Forward (+)
21351 .. 29064
7714 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00060.4

Sequence Viewer

Length: 4446 bp
ATGGAAAATCGCCATCTCCCTTCCAAATTCTTCTCCTCTGCTTCATCCATGGCTCAGAATCAACTTATCGATTCGCTAACATCTCACATATCTCTGTACCACTCCCACTCGTCTTCCTCTTCAATGGCTAATACTATCCCCAATCCTAGGTCGGCGATCCTCCGATGGTTCTCTTCTCTCAGCGTCCACCAGCGCCTCTCTCACCTCACCGTCGTTGATCCCAAGTTCGTCCAAATCCTCCTTCAGATGCTTGGTTATATCCGCACTAAAGGCCCTTGCTCTTTCATCATCCTCCCAGACCTCCCTTCCTCCTCCGATCTCCCCAGTCTCTGCTTTAAAAAATCTCGTGGCTTAATCTCCAGGGTTTCCGAGTCCAACGAGTCAGAGCGTTTCGTTTTCGACTCTACTCGGCTCTTTGGTTCTGGAGAAGGGGAAAGGGCTCAAGATTGCTCTTGCTCCGTCAATTCCCTCGATTCTGTCGTCATGGCCGAGGAATTTCTTACAAATGTGGATCGTTTCGTGGAGACTATGGACGTCTTATCGGATGGAGCATTTCTGAGAGGAGAAGAGAGTGATTTGGGGTCCAATTGGGTAGAATTAGAGTGGCTCAAAGCCAAAGGCTATTATAGCATGGAAGCCTTCGTTGCAAATAGGTTAGAGGTAAGCATGAGATTAGCTTGGCTGAACACAAATAGTGGCAAGAGAAGAGGAATCAAATTGAAAGAGAAATTGAATGCTGCTGCTGCGGCTGCCAATTCGTATTGGAGGAAGAAGGCCTGTGTTGATTGGTGGCAGAATTTGGATGCTGCAACACACAAGAAAATCTGGACTTGTTTGTTTGGGAAGTCGGCTAAATCTGTGATATATGAGATTCTTAGAGAAGCAAATCAAGCACAGCAGGGGGAAATGTGGCTCTTCAATTTTGCAAGTGCAAGGAAAGGGCGTACAGACACTTCTGCAGTTTCATTCTGTGACATGATTTTGGAACCGAACTCTGTACCAAGGAAACCAATAACCGTAGCCAGTAATCTAAGTGGCTTATACGTTCTTCAAGAATTTGCTTCACTGCTCATCTTATGCCAAAATGGCTTAGTTCCAGTACATAGTGTATTTTTCAGCTCAATGGGTACTATCACAACCTTAGTGGATTGCATCTTAAGAAAACTACGAGGTTTTCTTATGGTAATCTCTATTGATTCTGTTAAAAGTGAACTTCTTGACGACAACACTCACAAGTGTTCTCCAAGCTCATCATCAAATCAGAAGCTTGGTTCGACCAACCGTAAACAGAAAGGAAAGACCAGGAACATGAAAAAGCCAACTCCGGAGGCTAAATCTGATAAAAACGTTAATTTGTCCACGAAAAATGGAAAGAAAGATCAAGCTAAACTGGAATTTAACAAAAGCAGAGAAGCGATAGAATGCAAGAAAGTTCCTACAGCTTCGACGATGATCAATGATCCTGAGGCCTCTGCAGCAACTATGGAAGTTGTCCCAGGGTTAGTTGCCAGGAAAGGAAGAACTAAGAAGAAAAGGAAAGAAAAGAATAAGAGTAAGAAGTGTACAAGTTTGGAAAATAACGGGGAAGTGAATAAGTCTGTAGTGAATAGTTCAGCCATCGTAAAAGCTTCCAAATGTGATTCAAGTTGTACTTCAGCCAATCAGCATCCTCAGGAATATATCAATGCTCAGATTATTGAAGAGCATGGTTCATTTAGCTGTGAAAGGAATAGATCAGGTACTTGTGCATCTGTGAATGGTGCAGCTAATTGTGAGTATTCTGGAGAGGAAGAATCGCATAGCAAAGCTGAAACGCATGTGATTTCATCCGATTTGAGTTCTGTGGATCCTGCCGGTGGTCCAAGCTGTGAGAACGTGAACCCTCAAAAATCTTGCTGTCGGGGTGATCGTAAAGAAAAGTTGACAATGCCTAATGAACGATCCAGAACTTTGGATGAAGGGGAATCCCATCGGATTCACCATCAGAGAAGAGAAGCAGGTTACGGTTTTGCCTCCAGCAGTTCAGAATTTGTTTCTTATGAGTGGCCTGCTGTAGCTCCTATGTACTTTTCGCATGTTAGTTCTCACCTTCCAACTGCAACTGACAGACTGCACCTTGATGTTGGTCACAATTTGCATCCATACGTACGGCAACCTTTTGTGTCCACTGTGCAACATGCTAGAAATCCTTCAATTGAAGGTTCCCACAAACAAGTTCTCTCTCGACCCATGCCCATGAGTCTAGATTGGCCTCCCATGGTTCATAGCAATTGCGGCTTGACAACAGCATTCACTTGCAATTATGATTCTGGAATTTTGGTGGACATTCCTGAACAGAAAAATAAGCACGAGCTGGGGAATGAATGCGAGAATAACTGGATGTTAGAGGAAGATTTTGAGGTGCACACTGTTTCTGGAGTAGACTATAATCAATATTTCGGTGGTGGAGTGATGTATTGGAATCCTTCCGATCATCTCGGGACTGGCTTCTCTCGCCCTCCTTCCCTTAGTTCTGATGATAGCTCTTGGGCTTGGCATGAAGCTGAAATGAAGAGGTCTGTTGATGACATGGTTGCATTTTCGTCTTCTTACAGCGCAAATGGACTAGATTCTCCAACTGCAGCCTCGTTCTGTTCCCCTTTTCACCCCTTAGGCCCACCAAATCAGCCTCTCGGTTATGTTGTGCCAGGTAATGAAATATCTACCAAAATACTGCAAGCTCCGCCAACAACAATTGAAGGTGCAGGTGAAGAGGAAGTCTCCGGAACTTTGGCTAGTTTATCAGGGGATGTTGAAGGCAATTCAGGAGACTCACTTCCTTATCCAATTTTGCGCCCTATAATTATCCCAAATATGTCAAAATCTGAATACAAGCGTAGTTATGATACTAAAAGCCCAAACGTTCCACCTACAAGGCGCGAGCATCCTCGTATAAAGCGGCCACCGTCACCTGTGGTACTATGTGTTCCACGTGCTCCTCGACCACCACCTCCTTCTCCTGTGAGCAACTCCAGAGCACGGCGAGGTTTTCCAACTGTGAGGTCAGGAAGTTCAAGCCCAAGACATTGGGGTATGAGAGGCTGGTTTCATGACGGTGTAAACTGGGAAGAACCTCGTGGAGCAGAGATTGTTTTGCCCTGGAGAAACAAAAGCCTTGCAGTCAGGCCGATCATTCAGCCGTTACCGGGGGCCCTCCTGCAAGATCACTTAATTGCAATGTCGCAACTAGGCCGAGACCAAGAGCATCCTGATGTGGCCTTCCCTCTGCAACCACCCGAGTTATTGAACTGTCCAATGCAAGGGGAATCTCTCTCACTGATCCACGGTATTCTTAATGACGAGATAGATTCTTTCTGCAAGCAGGTTGCTGCAGAGAATATGGCCCGCAAACCTTATATCAATTGGGCAATCAAGCGGGTTACCCGATCTCTCCAAGTTCTGTGGCCCAGGTCTAGGACGAACATATTTGGTTCATCTGCAACTGGTTTGTCTCTCCCTTCTAGTGATGTGGATCTTGTGGTTTGTCTTCCTCCAGTGAGAAATTTAGAACCTATCAAAGAGGCAGGAATTTTGGAGGGTCGCAATGGTATAAAGGAGACTTGCCTTCAGCATGCAGCCAGGTATCTCGCGAATCAAGAGTGGGTAAAAACTGACTCCCTGAAGACAGTTGAAAACACAGCTATACCAATTATCATGCTTGTTGTGGAAGTCCCTTGTGATCTGATATGCAGTATACAGTCACCAAAAGATGGTCCAGACTGTATCACCGTTGACCAGGATAGCAATGGTAACACTGAAATGGTTGGATTTGAAGACTCTGCAGCAGCAAACTCTTTGCCAACAAACACTGGAAACTTGGCAATTGCAAAATCTGTCCGTCTTGACATCAGTTTCAAAACTCCATCCCATACGGGTCTTCAAACCACGCAGCTGGTCAAAGATCTGACTGAGCAATTTCCAGCTGCCACACCACTTGCTCTGGTGTTAAAGCAGTTTTTGGCTGATCGTACCCTGGATCAATCATACTCTGGCGGATTAAGCTCTTACTGCCTGGTCTTACTGATTACACGTTTTCTTCAGCATGAGCATCATTTGGGGCGCTCTATCAACCAAAACCTTGGGGGGCTTCTAATGGATTTTCTTTACTTTTTTGGTAATGTGTTTGATCCTCGCCAGATGCGTGTATCAGTGCAAGGAAGTGGTATCTATAGGAATCGGGAGAGGGGTTATAGTATTGATCCGATACACATTGATGATCCTCTTTTCCCAACAAATAATGTGGGTAGAAATTGCTTCCGTATACATCAATGTATTAAGGCATTTTCTGAAGCTTACTCTGTTCTGGAAAATGAGCTTACATGCATTACTAGTACCAGTGATTCGTGTGGGAAGCAGCAGCTGCACAATTTGCTTCCAAAAATCATCCCAAGTATCATTTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

1481

Amino Acids

163.8

Weight (kDa)

7.66

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 1136 - 1241 3.5e-08 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2735
AatII GACGTC 1 cut(s) 537
Acc36I ACCTGC 3 cut(s) 1988, 2735, 3352
AccB7I CCANNNNNTGG 1 cut(s) 3749
AccI GTMKAC 3 cut(s) 2421, 3733, 4300
AccII CGCG 2 cut(s) 2919, 3629
AccIII TCCGGA 2 cut(s) 1324, 2762
AciI CCGC 8 cut(s) 262, 746, 2274, 2723, 2938, 3385, 3415, 4032
AclI AACGTT 2 cut(s) 1347, 2901
AcoI YGGCCR 2 cut(s) 486, 2939
AcsI RAATTY 9 cut(s) 26, 494, 796, 1055, 1394, 2027, 2313, 3541, 3567
AcuI CTGAAG 6 cut(s) 227, 1638, 3590, 3680, 4061, 4347
AcvI CACGTG 1 cut(s) 2972
AcyI GRCGYC 1 cut(s) 534
AfiI CCNNNNNNNGG 7 cut(s) 147, 1856, 3018, 3185, 3299, 3453, 3749
AflII CTTAAG 1 cut(s) 1156
AflIII ACRYGT 1 cut(s) 4067
AhlI ACTAGT 1 cut(s) 4367
AjuI GAANNNNNNNTTGG 3 cut(s) 225, 257, 4420
Alw21I GWGCWC 3 cut(s) 2406, 2977, 3019
Alw26I GTCTC 7 cut(s) 332, 518, 2764, 2802, 3228, 3495, 3590
Alw44I GTGCAC 1 cut(s) 2402
AlwNI CAGNNNCTG 2 cut(s) 330, 4399
Ama87I CYCGRG 2 cut(s) 2477, 3275
Aor13HI TCCGGA 2 cut(s) 1324, 2762
ApaI GGGCCC 1 cut(s) 3193
ApaLI GTGCAC 1 cut(s) 2402
ApoI RAATTY 9 cut(s) 26, 494, 796, 1055, 1394, 2027, 2313, 3541, 3567
Asp700I GAANNNNTTC 1 cut(s) 2188
AspA2I CCTAGG 1 cut(s) 146
AspLEI GCGC 5 cut(s) 195, 2597, 2835, 2919, 4101
AspS9I GGNCC 9 cut(s) 272, 582, 1859, 2654, 3189, 3190, 3382, 3444, 3752
AsuC2I CCSGG 1 cut(s) 3186
AvaI CYCGRG 2 cut(s) 2477, 3275
AvaII GGWCC 3 cut(s) 582, 1859, 3752
AvrII CCTAGG 1 cut(s) 146
AxyI CCTNAGG 3 cut(s) 1464, 1671, 2650
BaeGI GKGCMC 2 cut(s) 2406, 3193
BamHI GGATCC 1 cut(s) 1846
BanII GRGCYC 2 cut(s) 442, 3193
BarI GAAGNNNNNNTAC 2 cut(s) 937, 969
BauI CACGAG 3 cut(s) 345, 2348, 3114
BbrPI CACGTG 1 cut(s) 2972
BbsI GAAGAC 6 cut(s) 105, 2577, 3518, 3668, 3819, 3908
Bbv12I GWGCWC 3 cut(s) 2406, 2977, 3019
BccI CCATC 8 cut(s) 21, 159, 539, 1625, 1977, 1989, 3743, 3910
BceAI ACGGC 3 cut(s) 2165, 3035, 3163
BclI TGATCA 1 cut(s) 1452
BcnI CCSGG 1 cut(s) 3186
BcoDI GTCTC 7 cut(s) 332, 518, 2764, 2802, 3228, 3495, 3590
BcuI ACTAGT 1 cut(s) 4367
BfaI CTAG 9 cut(s) 147, 2181, 2243, 2606, 2775, 3227, 3453, 3501, 4368
BfmI CTRYAG 9 cut(s) 957, 1437, 1473, 1599, 2052, 2619, 3369, 3819, 4207
BfoI RGCGCY 2 cut(s) 196, 4102
BfrI CTTAAG 1 cut(s) 1156
BfuAI ACCTGC 3 cut(s) 1988, 2735, 3352
BglI GCCNNNNNGGC 1 cut(s) 1086
BglII AGATCT 1 cut(s) 3940
BlnI CCTAGG 1 cut(s) 146
Bme18I GGWCC 3 cut(s) 582, 1859, 3752
BmeT110I CYCGRG 2 cut(s) 2477, 3275
BmgT120I GGNCC 9 cut(s) 272, 582, 1859, 2654, 3189, 3190, 3382, 3444, 3752
BmiI GGNNCC 6 cut(s) 583, 987, 1848, 2203, 3190, 3191
BmrI ACTGGG 2 cut(s) 318, 3112
BmuI ACTGGG 2 cut(s) 318, 3112
BpiI GAAGAC 6 cut(s) 105, 2577, 3518, 3668, 3819, 3908
BplI GAGNNNNNCTC 2 cut(s) 2744, 2776
BpmI CTGGAG 8 cut(s) 343, 444, 1803, 1999, 2436, 2995, 3160, 3516
BpuEI CTTGAG 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 3186
Bsa29I ATCGAT 1 cut(s) 69
BsaAI YACGTR 2 cut(s) 2146, 2972
BsaBI GATNNNNATC 1 cut(s) 3510
BsaHI GRCGYC 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 3228
BsaWI WCCGGW 2 cut(s) 1324, 2762
Bsc4I CCNNNNNNNGG 7 cut(s) 147, 1856, 3018, 3185, 3299, 3453, 3749
Bse118I RCCGGY 1 cut(s) 1853
Bse21I CCTNAGG 3 cut(s) 1464, 1671, 2650
Bse3DI GCAATG 3 cut(s) 3222, 3589, 3790
Bse8I GATNNNNATC 1 cut(s) 3510
BseAI TCCGGA 2 cut(s) 1324, 2762
BseCI ATCGAT 1 cut(s) 69
BseJI GATNNNNATC 1 cut(s) 3510
BseLI CCNNNNNNNGG 7 cut(s) 147, 1856, 3018, 3185, 3299, 3453, 3749
BseMI GCAATG 3 cut(s) 3222, 3589, 3790
BseMII CTCAG 7 cut(s) 68, 193, 548, 1455, 1685, 1703, 3939
BseRI GAGGAG 4 cut(s) 25, 301, 576, 2967
BseSI GKGCMC 2 cut(s) 2406, 3193
BseYI CCCAGC 1 cut(s) 2353
BsgI GTGCAG 4 cut(s) 1782, 2096, 2763, 4385
Bsh1236I CGCG 2 cut(s) 2919, 3629
BshVI ATCGAT 1 cut(s) 69
BsiHKAI GWGCWC 3 cut(s) 2406, 2977, 3019
BsiHKCI CYCGRG 2 cut(s) 2477, 3275
BsiSI CCGG 4 cut(s) 1325, 1854, 2763, 3185
BsiWI CGTACG 1 cut(s) 2146
BslFI GGGAC 3 cut(s) 1478, 2494, 3695
BslI CCNNNNNNNGG 7 cut(s) 147, 1856, 3018, 3185, 3299, 3453, 3749
BsmAI GTCTC 7 cut(s) 332, 518, 2764, 2802, 3228, 3495, 3590
BsmFI GGGAC 3 cut(s) 1478, 2494, 3695
BsmI GAATGC 4 cut(s) 739, 1427, 2288, 2369
Bso31I GGTCTC 1 cut(s) 3228
BsoBI CYCGRG 2 cut(s) 2477, 3275
Bsp120I GGGCCC 1 cut(s) 3189
Bsp1286I GDGCHC 5 cut(s) 442, 2406, 2977, 3019, 3193
Bsp13I TCCGGA 2 cut(s) 1324, 2762
Bsp1407I TGTACA 1 cut(s) 1562
Bsp19I CCATGG 2 cut(s) 48, 2256
Bsp68I TCGCGA 1 cut(s) 3629
BspACI CCGC 8 cut(s) 262, 746, 2274, 2723, 2938, 3385, 3415, 4032
BspCNI CTCAG 7 cut(s) 67, 192, 549, 1456, 1684, 1702, 3940
BspDI ATCGAT 1 cut(s) 69
BspEI TCCGGA 2 cut(s) 1324, 2762
BspFNI CGCG 2 cut(s) 2919, 3629
BspHI TCATGA 1 cut(s) 3088
BspLI GGNNCC 6 cut(s) 583, 987, 1848, 2203, 3190, 3191
BspMAI CTGCAG 5 cut(s) 961, 1477, 2623, 3373, 3823
BspMI ACCTGC 3 cut(s) 1988, 2735, 3352
BspQI GCTCTTC 2 cut(s) 920, 1695
BspTI CTTAAG 1 cut(s) 1156
BspTNI GGTCTC 1 cut(s) 3228
BsrDI GCAATG 3 cut(s) 3222, 3589, 3790
BsrFI RCCGGY 1 cut(s) 1853
BsrGI TGTACA 1 cut(s) 1562
BssAI RCCGGY 1 cut(s) 1853
BssNAI GTATAC 2 cut(s) 3734, 4301
BssNI GRCGYC 1 cut(s) 534
BssSI CACGAG 3 cut(s) 345, 2348, 3114
BssT1I CCWWGG 5 cut(s) 48, 146, 1001, 2256, 4117
Bst1107I GTATAC 2 cut(s) 3734, 4301
Bst2BI CACGAG 3 cut(s) 345, 2348, 3114
Bst6I CTCTTC 9 cut(s) 124, 178, 561, 700, 920, 1695, 1984, 2546, 2745
BstACI GRCGYC 1 cut(s) 534
BstAFI CTTAAG 1 cut(s) 1156
BstAPI GCANNNNNTGC 2 cut(s) 4399, 4408
BstAUI TGTACA 1 cut(s) 1562
BstBAI YACGTR 2 cut(s) 2146, 2972
BstC8I GCNNGC 6 cut(s) 2049, 2718, 2921, 3359, 3385, 3612
BstDSI CCRYGG 3 cut(s) 48, 2256, 3322
BstEII GGTNACC 1 cut(s) 3418
BstFNI CGCG 2 cut(s) 2919, 3629
BstH2I RGCGCY 2 cut(s) 196, 4102
BstHHI GCGC 5 cut(s) 195, 2597, 2835, 2919, 4101
BstMAI GTCTC 7 cut(s) 332, 518, 2764, 2802, 3228, 3495, 3590
BstNSI RCATGY 5 cut(s) 1820, 2078, 2180, 3614, 4362
BstPI GGTNACC 1 cut(s) 3418
BstSFI CTRYAG 9 cut(s) 957, 1437, 1473, 1599, 2052, 2619, 3369, 3819, 4207
BstSLI GKGCMC 2 cut(s) 2406, 3193
BstSNI TACGTA 1 cut(s) 2146
BstUI CGCG 2 cut(s) 2919, 3629
BstV2I GAAGAC 6 cut(s) 105, 2577, 3518, 3668, 3819, 3908
BstX2I RGATCY 3 cut(s) 1846, 3511, 3940
BstXI CCANNNNNNTGG 4 cut(s) 1951, 3066, 3931, 4118
BstYI RGATCY 3 cut(s) 1846, 3511, 3940
BstZ17I GTATAC 2 cut(s) 3734, 4301
Bsu15I ATCGAT 1 cut(s) 69
Bsu36I CCTNAGG 3 cut(s) 1464, 1671, 2650
BsuTUI ATCGAT 1 cut(s) 69
BtgI CCRYGG 3 cut(s) 48, 2256, 3322
BtsI GCAGTG 1 cut(s) 1064
BtsIMutI CAGTG 9 cut(s) 1064, 2166, 2406, 3314, 3540, 3792, 3846, 4194, 4381
BtuMI TCGCGA 1 cut(s) 3629
BveI ACCTGC 3 cut(s) 1988, 2735, 3352
Cac8I GCNNGC 6 cut(s) 2049, 2718, 2921, 3359, 3385, 3612
CaiI CAGNNNCTG 2 cut(s) 330, 4399
CciI TCATGA 1 cut(s) 3088
CfoI GCGC 5 cut(s) 195, 2597, 2835, 2919, 4101
Cfr10I RCCGGY 1 cut(s) 1853
Cfr13I GGNCC 9 cut(s) 272, 582, 1859, 2654, 3189, 3190, 3382, 3444, 3752
ClaI ATCGAT 1 cut(s) 69
CseI GACGC 1 cut(s) 172
CspCI CAANNNNNGTGG 8 cut(s) 1125, 1160, 3422, 3457, 3955, 3990, 4260, 4295
DraI TTTAAA 1 cut(s) 337
EaeI YGGCCR 2 cut(s) 486, 2939
Eam1104I CTCTTC 9 cut(s) 124, 178, 561, 700, 920, 1695, 1984, 2546, 2745
EarI CTCTTC 9 cut(s) 124, 178, 561, 700, 920, 1695, 1984, 2546, 2745
EciI GGCGGA 2 cut(s) 2712, 4047
Eco105I TACGTA 1 cut(s) 2146
Eco130I CCWWGG 5 cut(s) 48, 146, 1001, 2256, 4117
Eco147I AGGCCT 2 cut(s) 776, 1469
Eco24I GRGCYC 2 cut(s) 442, 3193
Eco31I GGTCTC 1 cut(s) 3228
Eco47I GGWCC 3 cut(s) 582, 1859, 3752
Eco57I CTGAAG 6 cut(s) 227, 1638, 3590, 3680, 4061, 4347
Eco72I CACGTG 1 cut(s) 2972
Eco81I CCTNAGG 3 cut(s) 1464, 1671, 2650
Eco88I CYCGRG 2 cut(s) 2477, 3275
Eco91I GGTNACC 1 cut(s) 3418
EcoO109I RGGNCCY 3 cut(s) 272, 3189, 3190
EcoO65I GGTNACC 1 cut(s) 3418
EcoT14I CCWWGG 5 cut(s) 48, 146, 1001, 2256, 4117
EcoT22I ATGCAT 1 cut(s) 4364
EcoT38I GRGCYC 2 cut(s) 442, 3193
ErhI CCWWGG 5 cut(s) 48, 146, 1001, 2256, 4117
FalI AAGNNNNNCTT 2 cut(s) 1636, 1668
FaqI GGGAC 3 cut(s) 1478, 2494, 3695
FauI CCCGC 2 cut(s) 3392, 3408
FbaI TGATCA 1 cut(s) 1452
FblI GTMKAC 3 cut(s) 2421, 3733, 4300
FriOI GRGCYC 2 cut(s) 442, 3193
FspBI CTAG 9 cut(s) 147, 2181, 2243, 2606, 2775, 3227, 3453, 3501, 4368
GlaI GCGC 5 cut(s) 194, 2596, 2834, 2918, 4100
GsaI CCCAGC 1 cut(s) 2357
GsuI CTGGAG 8 cut(s) 343, 444, 1803, 1999, 2436, 2995, 3160, 3516
HaeII RGCGCY 2 cut(s) 196, 4102
HapII CCGG 4 cut(s) 1325, 1854, 2763, 3185
HgaI GACGC 1 cut(s) 172
HhaI GCGC 5 cut(s) 195, 2597, 2835, 2919, 4101
Hin1I GRCGYC 1 cut(s) 534
Hin6I GCGC 5 cut(s) 193, 2595, 2833, 2917, 4099
HinP1I GCGC 5 cut(s) 193, 2595, 2833, 2917, 4099
HincII GTYRAC 2 cut(s) 1923, 3772
HindII GTYRAC 2 cut(s) 1923, 3772
HindIII AAGCTT 3 cut(s) 1265, 1626, 4329
HpaII CCGG 4 cut(s) 1325, 1854, 2763, 3185
Hpy99I CGWCG 2 cut(s) 215, 1450
HpyCH4IV ACGT 8 cut(s) 534, 1044, 1347, 1875, 2145, 2901, 2971, 4069
HpySE526I ACGT 8 cut(s) 534, 1044, 1347, 1875, 2145, 2901, 2971, 4069
Hsp92I GRCGYC 1 cut(s) 534
HspAI GCGC 5 cut(s) 193, 2595, 2833, 2917, 4099
Kpn2I TCCGGA 2 cut(s) 1324, 2762
Ksp22I TGATCA 1 cut(s) 1452
LguI GCTCTTC 2 cut(s) 920, 1695
LmnI GCTCC 6 cut(s) 461, 548, 2062, 2725, 2980, 3119
MaeI CTAG 9 cut(s) 147, 2181, 2243, 2606, 2775, 3227, 3453, 3501, 4368
MaeII ACGT 8 cut(s) 534, 1044, 1347, 1875, 2145, 2901, 2971, 4069
MaeIII GTNAC 8 cut(s) 971, 2000, 2126, 2946, 3180, 3418, 3738, 3788
MfeI CAATTG 6 cut(s) 586, 2193, 2269, 2733, 3400, 3861
MflI RGATCY 3 cut(s) 1846, 3511, 3940
MhlI GDGCHC 5 cut(s) 442, 2406, 2977, 3019, 3193
MlyI GAGTC 7 cut(s) 380, 389, 395, 2248, 2804, 3647, 3809
MmeI TCCRAC 5 cut(s) 399, 2117, 2639, 3056, 3784
Mph1103I ATGCAT 1 cut(s) 4364
MroI TCCGGA 2 cut(s) 1324, 2762
MroXI GAANNNNTTC 1 cut(s) 2188
MslI CAYNNNNRTG 6 cut(s) 2118, 2234, 3093, 3249, 3797, 4251
MspA1I CMGCKG 3 cut(s) 3931, 3962, 4399
MspCI CTTAAG 1 cut(s) 1156
MspI CCGG 4 cut(s) 1325, 1854, 2763, 3185
MunI CAATTG 6 cut(s) 586, 2193, 2269, 2733, 3400, 3861
Mva1269I GAATGC 4 cut(s) 739, 1427, 2288, 2369
MvnI CGCG 2 cut(s) 2919, 3629
NciI CCSGG 1 cut(s) 3186
NcoI CCATGG 2 cut(s) 48, 2256
NlaIV GGNNCC 6 cut(s) 583, 987, 1848, 2203, 3190, 3191
NmeAIII GCCGAG 3 cut(s) 388, 514, 3257
NmuCI GTSAC 4 cut(s) 971, 2126, 2946, 3738
NruI TCGCGA 1 cut(s) 3629
NsiI ATGCAT 1 cut(s) 4364
NspI RCATGY 5 cut(s) 1820, 2078, 2180, 3614, 4362
PaeI GCATGC 1 cut(s) 3614
PagI TCATGA 1 cut(s) 3088
PaqCI CACCTGC 1 cut(s) 2735
PasI CCCWGGG 1 cut(s) 1496
PceI AGGCCT 2 cut(s) 776, 1469
PciSI GCTCTTC 2 cut(s) 920, 1695
PcsI WCGNNNNNNNCGW 5 cut(s) 477, 486, 1906, 2977, 4177
PctI GAATGC 4 cut(s) 739, 1427, 2288, 2369
PdmI GAANNNNTTC 1 cut(s) 2188
Pfl23II CGTACG 1 cut(s) 2146
PflMI CCANNNNNTGG 1 cut(s) 3749
PleI GAGTC 7 cut(s) 379, 388, 395, 2247, 2804, 3647, 3809
PmaCI CACGTG 1 cut(s) 2972
PmlI CACGTG 1 cut(s) 2972
PpsI GAGTC 7 cut(s) 379, 388, 395, 2247, 2804, 3647, 3809
Ppu21I YACGTR 2 cut(s) 2146, 2972
Psp1406I AACGTT 2 cut(s) 1347, 2901
PspCI CACGTG 1 cut(s) 2972
PspEI GGTNACC 1 cut(s) 3418
PspFI CCCAGC 1 cut(s) 2353
PspLI CGTACG 1 cut(s) 2146
PspN4I GGNNCC 6 cut(s) 583, 987, 1848, 2203, 3190, 3191
PspOMI GGGCCC 1 cut(s) 3189
PspPI GGNCC 9 cut(s) 272, 582, 1859, 2654, 3189, 3190, 3382, 3444, 3752
PsrI GAACNNNNNNTAC 2 cut(s) 2949, 2981
PstI CTGCAG 5 cut(s) 961, 1477, 2623, 3373, 3823
PstNI CAGNNNCTG 2 cut(s) 330, 4399
PsuI RGATCY 3 cut(s) 1846, 3511, 3940
PvuII CAGCTG 3 cut(s) 3931, 3962, 4399
RruI TCGCGA 1 cut(s) 3629
RseI CAYNNNNRTG 6 cut(s) 2118, 2234, 3093, 3249, 3797, 4251
SapI GCTCTTC 2 cut(s) 920, 1695
Sau96I GGNCC 9 cut(s) 272, 582, 1859, 2654, 3189, 3190, 3382, 3444, 3752
SchI GAGTC 7 cut(s) 380, 389, 395, 2248, 2804, 3647, 3809
SduI GDGCHC 5 cut(s) 442, 2406, 2977, 3019, 3193
SfcI CTRYAG 9 cut(s) 957, 1437, 1473, 1599, 2052, 2619, 3369, 3819, 4207
SinI GGWCC 3 cut(s) 582, 1859, 3752
SmiMI CAYNNNNRTG 6 cut(s) 2118, 2234, 3093, 3249, 3797, 4251
SmlI CTYRAG 2 cut(s) 441, 1156
SmoI CTYRAG 2 cut(s) 441, 1156
SnaBI TACGTA 1 cut(s) 2146
SpeI ACTAGT 1 cut(s) 4367
SphI GCATGC 1 cut(s) 3614
SseBI AGGCCT 2 cut(s) 776, 1469
SsiI CCGC 8 cut(s) 262, 746, 2274, 2723, 2938, 3385, 3415, 4032
SspI AATATT 1 cut(s) 2435
SspMI CTAG 9 cut(s) 147, 2181, 2243, 2606, 2775, 3227, 3453, 3501, 4368
StuI AGGCCT 2 cut(s) 776, 1469
StyI CCWWGG 5 cut(s) 48, 146, 1001, 2256, 4117
TaiI ACGT 8 cut(s) 537, 1047, 1350, 1878, 2148, 2904, 2974, 4072
TaqI TCGA 7 cut(s) 69, 399, 471, 1274, 1445, 2224, 2980
TatI WGTACW 4 cut(s) 1099, 1562, 1649, 2064
TauI GCSGC 3 cut(s) 749, 2277, 2941
TscAI CASTG 9 cut(s) 1071, 2173, 2413, 3321, 3540, 3799, 3853, 4194, 4381
TseFI GTSAC 4 cut(s) 971, 2126, 2946, 3738
Tsp45I GTSAC 4 cut(s) 971, 2126, 2946, 3738
TspGWI ACGGA 3 cut(s) 448, 3866, 4286
TspRI CASTG 9 cut(s) 1071, 2173, 2413, 3321, 3540, 3799, 3853, 4194, 4381
Van91I CCANNNNNTGG 1 cut(s) 3749
Vha464I CTTAAG 1 cut(s) 1156
VneI GTGCAC 1 cut(s) 2402
VpaK11BI GGWCC 3 cut(s) 582, 1859, 3752
XapI RAATTY 9 cut(s) 26, 494, 796, 1055, 1394, 2027, 2313, 3541, 3567
XbaI TCTAGA 1 cut(s) 2242
XceI RCATGY 5 cut(s) 1820, 2078, 2180, 3614, 4362
XcmI CCANNNNNNNNNTGG 1 cut(s) 3782
XmaJI CCTAGG 1 cut(s) 146
XmiI GTMKAC 3 cut(s) 2421, 3733, 4300
XmnI GAANNNNTTC 1 cut(s) 2188
XspI CTAG 9 cut(s) 147, 2181, 2243, 2606, 2775, 3227, 3453, 3501, 4368
ZraI GACGTC 1 cut(s) 535
Zsp2I ATGCAT 1 cut(s) 4364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.