Rroxscaffold_4G00306210

Nucleotidyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003190
Physical Location & Seq
Forward (+)
26994780 .. 27008527
13748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306210.1

Sequence Viewer

Length: 4989 bp
ATGTCGAAACCAAACCCTAAATCCCCTTACAATTCCCGCATAACTCAGAACCAACTCATCGACTCCCTCACCTCCCACATCTCCCTCTACAATTCCCACTCCCTTTCCTCAGACCCCAAGCCCAACCCTAACCCTAGGTCCTCCATCCTCAAATGGTTCTCTTCTCTCTCCCTCCACCACCGCCAAGCTCACCTCACCGCCGTCGATCCCAAATTCGTCCGCCTCCTCCTCCAAATGCTCCGCAAGCTCCGCACGCAGGGCCACGGCTCCTTCATCATCCTCCCCGACCTCCCCTCCGGCGACCTCCCCACGCTCTGCTTCAAGAGGTCCGGCGGGCTGTTGTCTAGGGTTGCCGAGTCGAACGAGTCGGAGAAGATGGTGTTCGAGTCGACTCGGTTGTTTTCGTCGAGAGAGGGAGAGAAGGTTGAGGAGTGTTCGTGTTCGGTGAGGGATATTGATACGGTGACGGTGAGTGAGGAGTTGGTTGAGAATTTAGATAGGTTTGTGGAGGCTATGGATGATATATCAAATGGGGGATTCCTGAGAGGTGAAGACAGTGATTTGGGGTCGGATTGGGTGGAATTGAAGTGGTTGAAGGACAAAGGGTATTACAGTATGGAAGCTTTCATGGCGAACCGGTTAGAGGTTGCTCTGAGGCTTGCTTGGCTGAATTCGAATAATGCAAGGAAAAGAGGGGTGAAGTTGAAAGAGAAGATCAGCGCGGCGGGCGTGGCGGCCAATGTGTATTGGAGGAAGAAGGGGTGTGTGGACTGGTGGGGGAATTTGGATGATGCGATGCAGAGCAATGTAGTGACTTCAGTGTTGGGGAAAGCAGCGAAACCGTTGATTCATGAAATTCTGAAGGGAACAAGTAGTGGGGTAGAGGATGAAATGTGGCTCGCCAACACAGGAATGGAGCAACCATTGAGGTACAACCATAATGTATCTATGCGCAAGACTGTTCCAAAACTTGTGGCTGACACAGAATTTGGGTCAAGCATTATCCCAGCTTCTCTTTCTGGGAAACCTGCTTCCTTAGCCGATGCTTTTAATAATCTCTTTGTGCTTCAGGATATCATAACGATGATATCATTATGTCGCCATAATGAATATGATAAAGGGAAATTCTTCTATAGCACATTGAGTTCAATTACTACCGTTTCTGATTTTATACTAAGAAAAATACGAGGATTTCTCATGGTTCTTTTGCTTGACTGCACAAAACTTGAACTTTTAGCAGAGGGAAATGAAAAATGCTTACCTAAGAAACCCAAAGCAAAGCCTAGTGCTTCTAGCCGTAAAAGTAAGGGGAGGTCCTACAATATGAAAAGGCCAAATCCTGTTCCAAGGTCATGTACAGATGAATTTTTGTGTGAAACATCTGCCAAGGATCTTAGTACATTGGCTTATAAAGAGAAGGCAGATTCAGTGGAGTCCAAGTCGACGCATACTATACATCAAGAGAAGGAGATTTTCAGAGAGTTGTCATCATCAAAAGATGAAATGGAACATGCACAAGCATTGGCTGTTGGAAAAGTTCAGACAGCTGCGAGGAAGGGTAGGAAAGGAAAAGGCAAAAAGAAAAGAACTGGCTTGAGGAGTGCTAATGATATGGAGAAATTTGATAGATCATTCATGGAAGCTTCTTCTTCTTCTTCTTCGGTCATTCTTGAAGATGACGCTGCAAAGTCTGATCAGGTTTCTGGTGATACAGCCTACCAGAACATCAGATGTGATAATTCAGCTAGTTGTAACATCCCTTTGTCAAATTCAATTCCTTCTGGTTCTGCGAATGGACCTCTTAAAGATGAAGATGCCACCAAAAGCATTCAAGAGAATGATGGTATTGGTTCTAGTGCCACTTTCTGTCATAAAGTTCTGGAAAGTTACCAGTGTTTAAATAATAATACAGAAATCCAAACGAAATGTTCTGGATCAGAAACTGAGGCCTGTAAGGTAGATGGAAACTTGATAAAGTCTTCAGTGCCTGAGGTAGATGATAAAGTTTCCCATCCTAAAGATATTGACTTGCAGGACAAGCGTGCTGTTAAGTCAGGTGCGAAAGAAGTTTTGCCAGCCAAAGAAATAAGAGCTTCTGATGTAAATGAGGAAGCTGTTCTGTTGCAGGACCAAGAGAGTGGAAATAATATATATCACACTAGGGCTCCAAGTTCTTTTGATTGCCCTCCTTATGAGTGGCCGGGTGTAGCTTGTGCCTATTTTCCACCTGTTAACTCACATCTCCCACCTGCCACCGATCGATTGCATCTGGATGTTGGTCATAACTGGCAGAATCACCGCCAGTCTTTTCTACCCACAATACATCAAGTGAGAAACTCGGCAATTGAAGGTGGGTGTAATCCAGTTCTGACTCGACCATTGCCAATGAGTATAGATTGGCCCCCAATGGTTCGAAGTGCTCGTAGAGTGGCTCCATACCGGACCTGTAATTATGATTCTGGGTTCGTCTCAAAAAAGCAGTGTACTTATCCACGGGGGTTTGCACACACTGTCCAAATAAATGCAACAACCATGGATGATGAAAGGAAGTATTCTTGGGATTGCGCAGACCTACCTGATCCAACAAAGGCATATGAACTAGTGGATGAATGTGACAGTCACTGGATATCTGAGGATGAAGTTGAGGTGCAAGCATTTTCTGGGGCAGATTATAATCAATACTTTGGTGGTGGTGTGATGTACTGGAATCCTTCTGATCATACCGGGACAGTTTTCTCTCGCCCTCCTTCCCTTAGTTCTGATGATAGCTCATGGGCTTGGCGTGAAGCTGACATAAATAGGGCTGTTGATGATATGGTTGCTTTTTCTTCTCCATACAGTACAAATGGTTTGACTTCACCTACTGCTTCCTTCTGTTCTCCTTTTGATCCTTTGGGCTCAGGAAACCAGCCTCTTGGATATGTTATGTCAGGAAATGAAGTACCAGGCAAGGTGCTGCATCCCTCATCAACAATGGGAGACACAGTAGTAGATGAGGAATCCTCTGGATCTTTGGCTGACGTAACTGGTGATATTGAAGGGAAGACAGGTGATTCATTGCCTTACCCCATTTTGCGGCCAATCATCATATCAAGATCAAGAGACTTTAAGCGCAGCCATGATCATAAAAGCCCATGTGTTCCTCCTACTATGCGCGATCGACCTAGGATAAAGCGGCCACCGTCGCCGGTGGTGCTTTCTGTCCCACGCGCTCCACGACCACCTCCACCATCTTCTGTGAGTGACTCGAGGAAACACAGGGGATTTCCAACTGTTCGTTCTGGTAGCTCTAGCCCAAGGCACTGGGGTATGAGAGGTTGGTTCCATGATGGAGCTAACTTGGAGGAAGCTTGTTTACGCATGGATGGTGCTGAAGTTGTTTGGCCATTTAGAAATAATATTTCAGGCCGCCCATTGATTCAGCCTCTTCCTGCACCTTTGTTGCAGGATCGACTGATTGCTATTTCTCAACTAGCACGTGATCAGGAACATCCGGATGTTGCATTTCCCCTTCAGCCACCTGAGTTACATAACTGTCCAACACGGAAGGCATCTCTCTCTCTGATGCACAGCCTTGTGCATGATGAGATTGAATTTTTCTGCAAGAAGGTTGCTACGGAGAATATGTCTCGGAAGCCCTACATCAATTGGGCTGTCAAACGAGTTACACGGTCTCTCCAGGTGCTTTGGCCCAGATCCAGGACAAATATATTTGGTTCAGTTGCAAACGGTTTGTCCCTTCCAACTAGTGATGTGGACCTTGTGGTTTGTCTACCTCCTGTTAGGAATTTGGAACCCATTAAAGAAGCTGGGATATTGGAGGGTCGAAATGGTATTAAAGAGACATGCCTTCAGCATGCTGCCAGGTATCTTGTCAATCAGGATTGGGTTAAAAATGATTCTCTTAAGACCGTGGAAAATACAGCTATACCGATTATAATGCTTGTGGTGGAAGTTCCCAATGATCTAATTGCCTCATCTTCGTCTAACGTGCAATCACCAAAAGAAGAGGCACCTCACAGTACTGGTGAACCAGACTGTAATGTGCACTCCAGTGGGATTATTTTAGAAGAATCTGCTATGCCTAAGTGCCCCCAGATAACTTATGATGCTACAAAGGATTCAGTATCAATTCGCATTGACATCAGTTTCAAATCTCCATCTCATACAGGATTTCAAACTACACAGCTGGGAGCCTCTCTTTTGTTGAGTTTCATAACAGCCTTGGGCTCTCCTGCTGTGAATAAAAATAGTCATTTGCAGGTTAAAGATTTAACTGAGCAGTTTCCAGCCGCTACGCCCCTTGCTTTGGTACTGAAACAGTTCTTGGCGGATCGTAGTCTGGATCAGTCTTATTCTGGTGGCTTGAGTTCCTACTGCTTGGTGAGTAATGCTGGAACACCTCTTGTCGGGCTTAATGTACTACTAATTGTGCGTTTTCTCCAGCATGAGTATCATCTTGGCCGACCTATCAACCAAAACTTTGGAAACCTTCTCATGAATTTTCTTTATTTTTTTGGGAATGTTTTTGAGCCTCGGCAAATGCGTATTTCCGTACAGGGAAGTGGTGTTTATATAAAGAGGGAAAGAGGTTGCAGCATCGACCCCATACACATTGATGATCCCCTTTTTCCGACTAATAATGTCGGACGCAATTGCTTTCGGATACATCAGTGTATCAAGGCGTTTTCAGAAGCTTATTCTATTTTGGAGAATGAGCTTGCTTCTCTTCCTAATGATGATGATGCATCTTCAAGTCCCCCATATAGACTGCTTCCTAAAATCATCCCGAGTGGGCGGGAAGTTGACAAAAGTTACCGAGTAGTGGTGTCTACTTCTTTCATTGGAAAGGGGAAGTGGCATTATAGTAGGACCACGAGATTGAGCCATAATCATCACTGGGTACATTGCCTTTCAAAGTCTTGGTACGCTTATGAACTCTGCTCATATGGTTCTGCTACAGGAGTCAGCTACAGAAAGCAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

1662

Amino Acids

184.37

Weight (kDa)

7.39

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 1218 - 1314 1.6e-07 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1410, 2673, 3938
AarI CACCTGC 1 cut(s) 2257
Acc16I TGCGCA 2 cut(s) 953, 2566
Acc36I ACCTGC 3 cut(s) 1036, 2257, 4252
AccB1I GGYRCC 1 cut(s) 4012
AccI GTMKAC 4 cut(s) 389, 1442, 3772, 4832
AccII CGCG 3 cut(s) 722, 3156, 3210
AccIII TCCGGA 1 cut(s) 3493
AcoI YGGCCR 6 cut(s) 735, 2198, 3077, 3176, 3383, 4462
AcuI CTGAAG 7 cut(s) 801, 881, 1052, 1965, 3393, 3497, 3836
AcvI CACGTG 1 cut(s) 3479
AfiI CCNNNNNNNGG 7 cut(s) 256, 643, 3075, 3699, 4309, 4409, 4825
AflII CTTAAG 1 cut(s) 3905
AgeI ACCGGT 1 cut(s) 636
AhlI ACTAGT 2 cut(s) 2599, 3746
AjnI CCWGG 4 cut(s) 2944, 3678, 3698, 3863
AjuI GAANNNNNNNTTGG 4 cut(s) 3262, 3294, 4310, 4342
AleI CACNNNNGTG 1 cut(s) 4053
Alw21I GWGCWC 2 cut(s) 2422, 4050
Alw26I GTCTC 6 cut(s) 2473, 2973, 3096, 3633, 3678, 3836
Alw44I GTGCAC 1 cut(s) 4046
AlwNI CAGNNNCTG 3 cut(s) 1943, 1988, 2622
Ama87I CYCGRG 2 cut(s) 3247, 4789
Aor13HI TCCGGA 1 cut(s) 3493
ApaLI GTGCAC 1 cut(s) 4046
ApeKI GCWGC 7 cut(s) 833, 1547, 1682, 2956, 3114, 3860, 4596
ArsI GACNNNNNNTTYG 4 cut(s) 971, 1003, 3719, 3751
AsiGI ACCGGT 1 cut(s) 636
Asp700I GAANNNNTTC 3 cut(s) 1127, 2115, 4322
AspA2I CCTAGG 2 cut(s) 134, 3164
AspLEI GCGC 6 cut(s) 722, 954, 2567, 3114, 3156, 3212
AsuC2I CCSGG 2 cut(s) 2202, 2725
AsuII TTCGAA 2 cut(s) 674, 2413
AvaI CYCGRG 2 cut(s) 3247, 4789
AvaII GGWCC 8 cut(s) 138, 327, 1314, 1796, 2128, 2442, 3757, 4872
AvrII CCTAGG 2 cut(s) 134, 3164
AxyI CCTNAGG 1 cut(s) 1989
BaeGI GKGCMC 2 cut(s) 4050, 4094
BaeI ACNNNNGTAYC 2 cut(s) 4658, 4691
BalI TGGCCA 1 cut(s) 3385
BanI GGYRCC 1 cut(s) 4012
BanII GRGCYC 3 cut(s) 2167, 2900, 4232
BarI GAAGNNNNNNTAC 2 cut(s) 3339, 3371
BauI CACGAG 1 cut(s) 4876
BbrPI CACGTG 1 cut(s) 3479
BbsI GAAGAC 3 cut(s) 558, 1971, 3050
Bbv12I GWGCWC 2 cut(s) 2422, 4050
BbvI GCAGC 7 cut(s) 845, 1534, 1669, 2943, 3126, 3847, 4608
BccI CCATC 9 cut(s) 152, 370, 1835, 1955, 2019, 3238, 3323, 3359, 4168
BceAI ACGGC 3 cut(s) 185, 280, 1281
BcgI CGANNNNNNTGC 2 cut(s) 3922, 3956
BciT130I CCWGG 4 cut(s) 2946, 3680, 3700, 3865
BciVI GTATCC 1 cut(s) 4659
BclI TGATCA 4 cut(s) 1693, 2716, 3121, 3481
BcnI CCSGG 2 cut(s) 2202, 2725
BcoDI GTCTC 6 cut(s) 2473, 2973, 3096, 3633, 3678, 3836
BcuI ACTAGT 2 cut(s) 2599, 3746
BfmI CTRYAG 3 cut(s) 1132, 4959, 4972
BfrI CTTAAG 1 cut(s) 3905
BfuAI ACCTGC 3 cut(s) 1036, 2257, 4252
BfuI GTATCC 1 cut(s) 4659
BlnI CCTAGG 2 cut(s) 134, 3164
BmcAI AGTACT 1 cut(s) 4024
Bme1390I CCNGG 6 cut(s) 2202, 2725, 2946, 3680, 3700, 3865
Bme18I GGWCC 8 cut(s) 138, 327, 1314, 1796, 2128, 2442, 3757, 4872
BmeT110I CYCGRG 2 cut(s) 3247, 4789
BmiI GGNNCC 8 cut(s) 268, 2166, 2402, 2433, 3323, 3795, 4014, 4195
BmrFI CCNGG 6 cut(s) 2202, 2725, 2946, 3680, 3700, 3865
BmrI ACTGGG 2 cut(s) 3313, 4909
BmuI ACTGGG 2 cut(s) 3313, 4909
BpiI GAAGAC 3 cut(s) 558, 1971, 3050
BplI GAGNNNNNCTC 4 cut(s) 1179, 1211, 2987, 3019
BpmI CTGGAG 3 cut(s) 3662, 4036, 4427
Bpu10I CCTNAGC 2 cut(s) 1036, 2899
Bpu14I TTCGAA 2 cut(s) 674, 2413
BpuEI CTTGAG 2 cut(s) 1615, 4387
BpuMI CCSGG 2 cut(s) 2202, 2725
Bsa29I ATCGAT 1 cut(s) 2260
BsaAI YACGTR 1 cut(s) 3479
BsaBI GATNNNNATC 1 cut(s) 2673
BsaI GGTCTC 1 cut(s) 3678
BsaWI WCCGGW 3 cut(s) 636, 2439, 3493
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 643, 3075, 3699, 4309, 4409, 4825
Bse118I RCCGGY 2 cut(s) 636, 3187
Bse21I CCTNAGG 1 cut(s) 1989
Bse3DI GCAATG 4 cut(s) 811, 2378, 3056, 4906
Bse8I GATNNNNATC 1 cut(s) 2673
BseAI TCCGGA 1 cut(s) 3493
BseBI CCWGG 4 cut(s) 2946, 3680, 3700, 3865
BseCI ATCGAT 1 cut(s) 2260
BseJI GATNNNNATC 1 cut(s) 2673
BseLI CCNNNNNNNGG 7 cut(s) 256, 643, 3075, 3699, 4309, 4409, 4825
BseMI GCAATG 4 cut(s) 811, 2378, 3056, 4906
BseRI GAGGAG 5 cut(s) 215, 218, 443, 491, 1612
BseSI GKGCMC 2 cut(s) 4050, 4094
BseXI GCAGC 7 cut(s) 845, 1534, 1669, 2943, 3126, 3847, 4608
BseYI CCCAGC 3 cut(s) 1006, 3809, 4189
BsgI GTGCAG 2 cut(s) 1201, 3417
Bsh1236I CGCG 3 cut(s) 722, 3156, 3210
Bsh1285I CGRYCG 2 cut(s) 2260, 3160
BshNI GGYRCC 1 cut(s) 4012
BshTI ACCGGT 1 cut(s) 636
BshVI ATCGAT 1 cut(s) 2260
BsiEI CGRYCG 2 cut(s) 2260, 3160
BsiHKAI GWGCWC 2 cut(s) 2422, 4050
BsiHKCI CYCGRG 2 cut(s) 3247, 4789
BsiSI CCGG 8 cut(s) 297, 330, 637, 2201, 2440, 2724, 3188, 3494
BslFI GGGAC 4 cut(s) 2740, 3188, 3721, 4743
BslI CCNNNNNNNGG 7 cut(s) 256, 643, 3075, 3699, 4309, 4409, 4825
BsmAI GTCTC 6 cut(s) 2473, 2973, 3096, 3633, 3678, 3836
BsmBI CGTCTC 1 cut(s) 2473
BsmFI GGGAC 4 cut(s) 2740, 3188, 3721, 4743
BsmI GAATGC 1 cut(s) 1827
Bso31I GGTCTC 1 cut(s) 3678
BsoBI CYCGRG 2 cut(s) 3247, 4789
Bsp119I TTCGAA 2 cut(s) 674, 2413
Bsp1286I GDGCHC 6 cut(s) 2167, 2422, 2900, 4050, 4094, 4232
Bsp13I TCCGGA 1 cut(s) 3493
Bsp1407I TGTACA 1 cut(s) 1355
Bsp19I CCATGG 1 cut(s) 2532
BspDI ATCGAT 1 cut(s) 2260
BspEI TCCGGA 1 cut(s) 3493
BspFNI CGCG 3 cut(s) 722, 3156, 3210
BspHI TCATGA 2 cut(s) 850, 4497
BspLI GGNNCC 8 cut(s) 268, 2166, 2402, 2433, 3323, 3795, 4014, 4195
BspMI ACCTGC 3 cut(s) 1036, 2257, 4252
BspT104I TTCGAA 2 cut(s) 674, 2413
BspT107I GGYRCC 1 cut(s) 4012
BspTI CTTAAG 1 cut(s) 3905
BspTNI GGTCTC 1 cut(s) 3678
BsrDI GCAATG 4 cut(s) 811, 2378, 3056, 4906
BsrFI RCCGGY 2 cut(s) 636, 3187
BsrGI TGTACA 1 cut(s) 1355
BssAI RCCGGY 2 cut(s) 636, 3187
BssSI CACGAG 1 cut(s) 4876
BssT1I CCWWGG 7 cut(s) 134, 1346, 1386, 2532, 3164, 3296, 4224
Bst2BI CACGAG 1 cut(s) 4876
Bst2UI CCWGG 4 cut(s) 2946, 3680, 3700, 3865
Bst6I CTCTTC 4 cut(s) 166, 3432, 4002, 4734
BstAFI CTTAAG 1 cut(s) 3905
BstAUI TGTACA 1 cut(s) 1355
BstBAI YACGTR 1 cut(s) 3479
BstBI TTCGAA 2 cut(s) 674, 2413
BstDSI CCRYGG 4 cut(s) 262, 2492, 2532, 3912
BstFNI CGCG 3 cut(s) 722, 3156, 3210
BstHHI GCGC 6 cut(s) 722, 954, 2567, 3114, 3156, 3212
BstMAI GTCTC 6 cut(s) 2473, 2973, 3096, 3633, 3678, 3836
BstMCI CGRYCG 2 cut(s) 2260, 3160
BstNI CCWGG 4 cut(s) 2946, 3680, 3700, 3865
BstNSI RCATGY 3 cut(s) 1514, 3849, 3860
BstSCI CCNGG 6 cut(s) 2200, 2723, 2944, 3678, 3698, 3863
BstSFI CTRYAG 3 cut(s) 1132, 4959, 4972
BstSLI GKGCMC 2 cut(s) 4050, 4094
BstUI CGCG 3 cut(s) 722, 3156, 3210
BstV1I GCAGC 7 cut(s) 845, 1534, 1669, 2943, 3126, 3847, 4608
BstV2I GAAGAC 3 cut(s) 558, 1971, 3050
BstX2I RGATCY 3 cut(s) 1390, 3008, 3695
BstXI CCANNNNNNTGG 4 cut(s) 2138, 2915, 3303, 4484
BstYI RGATCY 3 cut(s) 1390, 3008, 3695
Bsu15I ATCGAT 1 cut(s) 2260
Bsu36I CCTNAGG 1 cut(s) 1989
BsuI GTATCC 1 cut(s) 4659
BsuTUI ATCGAT 1 cut(s) 2260
BtgI CCRYGG 4 cut(s) 262, 2492, 2532, 3912
BtgZI GCGATG 1 cut(s) 809
BtsI GCAGTG 1 cut(s) 2486
BveI ACCTGC 3 cut(s) 1036, 2257, 4252
CaiI CAGNNNCTG 3 cut(s) 1943, 1988, 2622
CciI TCATGA 2 cut(s) 850, 4497
CfoI GCGC 6 cut(s) 722, 954, 2567, 3114, 3156, 3212
Cfr10I RCCGGY 2 cut(s) 636, 3187
ClaI ATCGAT 1 cut(s) 2260
CseI GACGC 3 cut(s) 1453, 1688, 4659
CspAI ACCGGT 1 cut(s) 636
CspCI CAANNNNNGTGG 2 cut(s) 954, 989
DraI TTTAAA 1 cut(s) 1899
EaeI YGGCCR 6 cut(s) 735, 2198, 3077, 3176, 3383, 4462
Eam1104I CTCTTC 4 cut(s) 166, 3432, 4002, 4734
EarI CTCTTC 4 cut(s) 166, 3432, 4002, 4734
EciI GGCGGA 2 cut(s) 209, 4346
Eco130I CCWWGG 7 cut(s) 134, 1346, 1386, 2532, 3164, 3296, 4224
Eco147I AGGCCT 1 cut(s) 1949
Eco24I GRGCYC 3 cut(s) 2167, 2900, 4232
Eco31I GGTCTC 1 cut(s) 3678
Eco32I GATATC 3 cut(s) 1075, 1089, 2628
Eco47I GGWCC 8 cut(s) 138, 327, 1314, 1796, 2128, 2442, 3757, 4872
Eco57I CTGAAG 7 cut(s) 801, 881, 1052, 1965, 3393, 3497, 3836
Eco72I CACGTG 1 cut(s) 3479
Eco81I CCTNAGG 1 cut(s) 1989
Eco88I CYCGRG 2 cut(s) 3247, 4789
EcoO109I RGGNCCY 2 cut(s) 138, 1314
EcoRI GAATTC 1 cut(s) 670
EcoRII CCWGG 4 cut(s) 2944, 3678, 3698, 3863
EcoRV GATATC 3 cut(s) 1075, 1089, 2628
EcoT14I CCWWGG 7 cut(s) 134, 1346, 1386, 2532, 3164, 3296, 4224
EcoT22I ATGCAT 1 cut(s) 4750
EcoT38I GRGCYC 3 cut(s) 2167, 2900, 4232
ErhI CCWWGG 7 cut(s) 134, 1346, 1386, 2532, 3164, 3296, 4224
Esp3I CGTCTC 1 cut(s) 2473
FaqI GGGAC 4 cut(s) 2740, 3188, 3721, 4743
FauI CCCGC 4 cut(s) 44, 326, 718, 4791
FauNDI CATATG 2 cut(s) 2593, 4948
FbaI TGATCA 4 cut(s) 1693, 2716, 3121, 3481
FblI GTMKAC 4 cut(s) 389, 1442, 3772, 4832
FriOI GRGCYC 3 cut(s) 2167, 2900, 4232
FspI TGCGCA 2 cut(s) 953, 2566
GlaI GCGC 6 cut(s) 721, 953, 2566, 3113, 3155, 3211
GsaI CCCAGC 3 cut(s) 1010, 3813, 4193
GsuI CTGGAG 3 cut(s) 3662, 4036, 4427
HapII CCGG 8 cut(s) 297, 330, 637, 2201, 2440, 2724, 3188, 3494
HgaI GACGC 3 cut(s) 1453, 1688, 4659
HhaI GCGC 6 cut(s) 722, 954, 2567, 3114, 3156, 3212
Hin6I GCGC 6 cut(s) 720, 952, 2565, 3112, 3154, 3210
HinP1I GCGC 6 cut(s) 720, 952, 2565, 3112, 3154, 3210
HincII GTYRAC 4 cut(s) 390, 1443, 2233, 4807
HindII GTYRAC 4 cut(s) 390, 1443, 2233, 4807
HindIII AAGCTT 4 cut(s) 621, 1641, 3348, 4695
HpaI GTTAAC 1 cut(s) 2233
HpaII CCGG 8 cut(s) 297, 330, 637, 2201, 2440, 2724, 3188, 3494
Hpy99I CGWCG 4 cut(s) 206, 409, 1447, 3187
HpyCH4IV ACGT 3 cut(s) 3021, 3478, 3990
HpySE526I ACGT 3 cut(s) 3021, 3478, 3990
HspAI GCGC 6 cut(s) 720, 952, 2565, 3112, 3154, 3210
Kpn2I TCCGGA 1 cut(s) 3493
Ksp22I TGATCA 4 cut(s) 1693, 2716, 3121, 3481
KspAI GTTAAC 1 cut(s) 2233
LmnI GCTCC 9 cut(s) 243, 252, 272, 916, 2170, 2437, 3217, 3332, 4193
Lsp1109I GCAGC 7 cut(s) 845, 1534, 1669, 2943, 3126, 3847, 4608
MaeII ACGT 3 cut(s) 3021, 3478, 3990
MfeI CAATTG 3 cut(s) 2343, 3646, 4654
MflI RGATCY 3 cut(s) 1390, 3008, 3695
MhlI GDGCHC 6 cut(s) 2167, 2422, 2900, 4050, 4094, 4232
MlsI TGGCCA 1 cut(s) 3385
MluNI TGGCCA 1 cut(s) 3385
MlyI GAGTC 9 cut(s) 56, 365, 374, 385, 395, 1442, 2365, 3239, 4974
MmeI TCCRAC 9 cut(s) 348, 549, 1510, 2606, 3293, 3563, 3767, 4627, 4658
Mox20I TGGCCA 1 cut(s) 3385
Mph1103I ATGCAT 1 cut(s) 4750
MroI TCCGGA 1 cut(s) 3493
MroXI GAANNNNTTC 3 cut(s) 1127, 2115, 4322
MscI TGGCCA 1 cut(s) 3385
MslI CAYNNNNRTG 6 cut(s) 911, 1082, 2271, 3495, 4053, 4617
Msp20I TGGCCA 1 cut(s) 3385
MspA1I CMGCKG 2 cut(s) 1547, 4189
MspCI CTTAAG 1 cut(s) 3905
MspI CCGG 8 cut(s) 297, 330, 637, 2201, 2440, 2724, 3188, 3494
MspR9I CCNGG 6 cut(s) 2202, 2725, 2946, 3680, 3700, 3865
MunI CAATTG 3 cut(s) 2343, 3646, 4654
Mva1269I GAATGC 1 cut(s) 1827
MvaI CCWGG 4 cut(s) 2946, 3680, 3700, 3865
MvnI CGCG 3 cut(s) 722, 3156, 3210
NciI CCSGG 2 cut(s) 2202, 2725
NcoI CCATGG 1 cut(s) 2532
NdeI CATATG 2 cut(s) 2593, 4948
NlaIV GGNNCC 8 cut(s) 268, 2166, 2402, 2433, 3323, 3795, 4014, 4195
NmeAIII GCCGAG 3 cut(s) 379, 2318, 4516
NmuCI GTSAC 5 cut(s) 463, 811, 2612, 2618, 3242
NsbI TGCGCA 2 cut(s) 953, 2566
NsiI ATGCAT 1 cut(s) 4750
NspI RCATGY 3 cut(s) 1514, 3849, 3860
NspV TTCGAA 2 cut(s) 674, 2413
OliI CACNNNNGTG 1 cut(s) 4053
PaeI GCATGC 1 cut(s) 3860
PaeR7I CTCGAG 1 cut(s) 3247
PagI TCATGA 2 cut(s) 850, 4497
PaqCI CACCTGC 1 cut(s) 2257
PceI AGGCCT 1 cut(s) 1949
PcsI WCGNNNNNNNCGW 3 cut(s) 2419, 3214, 4543
PctI GAATGC 1 cut(s) 1827
PdmI GAANNNNTTC 3 cut(s) 1127, 2115, 4322
PfoI TCCNGGA 1 cut(s) 3698
PinAI ACCGGT 1 cut(s) 636
Ple19I CGATCG 2 cut(s) 2260, 3160
PleI GAGTC 9 cut(s) 56, 364, 373, 385, 394, 1441, 2365, 3239, 4973
PmaCI CACGTG 1 cut(s) 3479
PmlI CACGTG 1 cut(s) 3479
PpsI GAGTC 9 cut(s) 56, 364, 373, 385, 394, 1441, 2365, 3239, 4973
Ppu21I YACGTR 1 cut(s) 3479
PpuMI RGGWCCY 2 cut(s) 138, 1314
PsiI TTATAA 3 cut(s) 1410, 2673, 3938
Psp5II RGGWCCY 2 cut(s) 138, 1314
Psp6I CCWGG 4 cut(s) 2944, 3678, 3698, 3863
PspCI CACGTG 1 cut(s) 3479
PspFI CCCAGC 3 cut(s) 1006, 3809, 4189
PspGI CCWGG 4 cut(s) 2944, 3678, 3698, 3863
PspN4I GGNNCC 8 cut(s) 268, 2166, 2402, 2433, 3323, 3795, 4014, 4195
PspPPI RGGWCCY 2 cut(s) 138, 1314
PspXI VCTCGAGB 1 cut(s) 3247
PstNI CAGNNNCTG 3 cut(s) 1943, 1988, 2622
PsuI RGATCY 3 cut(s) 1390, 3008, 3695
PvuI CGATCG 2 cut(s) 2260, 3160
PvuII CAGCTG 2 cut(s) 1547, 4189
RseI CAYNNNNRTG 6 cut(s) 911, 1082, 2271, 3495, 4053, 4617
SalI GTCGAC 2 cut(s) 388, 1441
ScaI AGTACT 1 cut(s) 4024
SchI GAGTC 9 cut(s) 56, 365, 374, 385, 395, 1442, 2365, 3239, 4974
ScrFI CCNGG 6 cut(s) 2202, 2725, 2946, 3680, 3700, 3865
SduI GDGCHC 6 cut(s) 2167, 2422, 2900, 4050, 4094, 4232
SfcI CTRYAG 3 cut(s) 1132, 4959, 4972
Sfr274I CTCGAG 1 cut(s) 3247
SfuI TTCGAA 2 cut(s) 674, 2413
SgrAI CRCCGGYG 1 cut(s) 3187
SinI GGWCC 8 cut(s) 138, 327, 1314, 1796, 2128, 2442, 3757, 4872
SlaI CTCGAG 1 cut(s) 3247
SmiMI CAYNNNNRTG 6 cut(s) 911, 1082, 2271, 3495, 4053, 4617
SmlI CTYRAG 4 cut(s) 1594, 3247, 3905, 4366
SmoI CTYRAG 4 cut(s) 1594, 3247, 3905, 4366
SpeI ACTAGT 2 cut(s) 2599, 3746
SphI GCATGC 1 cut(s) 3860
SseBI AGGCCT 1 cut(s) 1949
SspI AATATT 1 cut(s) 3400
StuI AGGCCT 1 cut(s) 1949
StyD4I CCNGG 6 cut(s) 2200, 2723, 2944, 3678, 3698, 3863
StyI CCWWGG 7 cut(s) 134, 1346, 1386, 2532, 3164, 3296, 4224
TaiI ACGT 3 cut(s) 3024, 3481, 3993
TaqII GACCGA 1 cut(s) 1651
TatI WGTACW 7 cut(s) 1355, 1397, 2483, 2700, 2840, 4022, 4420
TauI GCSGC 6 cut(s) 725, 737, 3079, 3178, 3411, 4295
TseFI GTSAC 5 cut(s) 463, 811, 2612, 2618, 3242
TseI GCWGC 7 cut(s) 833, 1547, 1682, 2956, 3114, 3860, 4596
Tsp45I GTSAC 5 cut(s) 463, 811, 2612, 2618, 3242
TspGWI ACGGA 3 cut(s) 3559, 3632, 4543
Vha464I CTTAAG 1 cut(s) 3905
VneI GTGCAC 1 cut(s) 4046
VpaK11BI GGWCC 8 cut(s) 138, 327, 1314, 1796, 2128, 2442, 3757, 4872
XceI RCATGY 3 cut(s) 1514, 3849, 3860
XcmI CCANNNNNNNNNTGG 1 cut(s) 910
XhoI CTCGAG 1 cut(s) 3247
XmaJI CCTAGG 2 cut(s) 134, 3164
XmiI GTMKAC 4 cut(s) 389, 1442, 3772, 4832
XmnI GAANNNNTTC 3 cut(s) 1127, 2115, 4322
ZrmI AGTACT 1 cut(s) 4024
Zsp2I ATGCAT 1 cut(s) 4750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.