FvH4_7g11041

Nucleotidyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003190
Physical Location & Seq
Reverse (-)
10342489 .. 10355002
12514 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11041.t1

Sequence Viewer

Length: 3843 bp
ATGTGGCTCCTCAACACAGGAATGGAGCAACCATTGAGGTACAACCATATTGTGTCTATGCGCAAGACTGTTCCAAAACTTGTGGCTGACACAGAATTTGGGTCAAGCATTATCCCAGCTTCTCTTTCTGGGAAACCTGCTTCCTTAGCCGATGCATTTAATAATCTCTTTGTGCTTCAGGATATCATAAAGATGATATCATTATGTTGCAATAATGAATATGATAAAGGGAAATTCTTCTATAGCACATTGAGTTCAATTTCTACCATTTCTGATTTTATACTAAGAAAATTACGAGGATTTCTCATGGTTCTCTTGCTTGACTGCACAAAACTTGAACTTTTATCAGAGGGAAATGAAAAATGCTTATCTAAGAAAACCAAAGCAAAGCCTAGTGCTTCTAGCCGTAAAAGTAAAGGGAGGGCCAGCAATATGAAAAGGCCAAATCCTGTTCCAATGTCATGTACAGATGAAGTTTTATGTGAAACGTCTGCCAAGGATCTCAGTGTGTTGGCTCATAAAGAGAAGGCAGATTCGGTGGAGTCCAAGAAGACGCATGATAAACATCAAGAGGTGGAGATTTTCAAGGAGTCATCATCATCGAAACATGAAATGGAACATGCACAAGCATTGGCTGTTGCAAAAGTTCAGACAGCTGCGAGGAAGGGTAGGAAAGGGAAAGGCAAAAAGAAAATAACTGGCTTGAGGAATGCTGATGATATGGATAAATTAGAGAGATCAGTCGCGGAAGCTTCTTCTTCTTCTTCTTCGGTTATTGCCAAAGATACCACTGCAAAGTCAGATCGAACTTTTGGTGATACAGCCTTCCAGAACATATTTGATAATTCAGCTAGTTGTAACAACCCTTTGCCAAATTCAATTCCTTGTGGTACTGCGAATGGACCTCTTAGAGATGAAGATGCCACCAAAAGCAGTCAAGAGAACGATGGTATTGGTTCCAATTTATGTCATAAAGTTTCGGGAAGTTACCAGTCTTCAAATAATATTACTGAAATCCAAAAGAAATGTCCTGGATCAGAAGCTGAAGCCTGTAAGGTAGATGGAATCATGATAGAGTCTTCAGTGCCTGAGGTAGGTAAAATTGTGATAAAGTCTTCAGTGCCTGAGGTAGATGATACAGTTTCCCATCGTAAAGATATTGATAGGCTGGAAAAGCACGCTGTTAAGTCTGGTGTGAAAGAAGTTTTGCCAGAGAAAGAAATAAGAGCTTCGGATGTAAACCAAGAAGCTGTTCTGTTGCAGGACCAAGAGAATGGAAATAATTTATATCATACTAGAACTCCAAGTGCTTTTGAATGCCCTCCTTATGAGTGGCCTGGTGTAGCTTGTGCCTATTTTCCACCTGTTAACTCACATCTCCCACCTGCCACTGATCGATTGCATCTGGATGTCGGTCATAACTGGCAGAATCACCGCCAGTCTTTTCTACCGACAATACATCAGGTGAGAAACTCGGCAATCGAAGGTGGGTGTAATCCAGTTCTGACTCGACCACTGCCAATGAGTATAGATTGGCCCCCAATGATTCGAAGTGCTCGTAGAGTGGCTCCGTACCGGACCTGTAATTATGATTCTGGTTTTGTCTCAAAAAAGCAGTTTACTTTTCCACAGGGTTTTACACACACTGTCCAAATAAATGCAACAACCATTGATGATGAAAGGAAGTATTCTTGGGATTGTGCAGACCTACCTGATCCAACAAAGGCATATGAACTAGTGGATGAATGTGACAGCCACTGGATATCTGAGGATGAAGTTGAGGTCCAAGCATTTTCTGGGGCAGATTATAATCAGTACTTTGGTGGTGGTGTGATGTACTGGAATCCTTCTGATAATACCGGGACGGTTTTCTCTCGCCCCCCTTCCCTTAGTTCTGATGATAGCTCATGGGCTTGGCGTGAAGCTGACATAAATAGGGCTGTTGATGATATGGTTGCATTTTCTTCTCCTTTCAGTACGAATGGTTTGACTTCGCCTACTGCTTCCTTCTGTTCTCCTTTTGAACCTTTGGGATCTGGAAACCAGCCTCTTGGATATGTTATGTCAGGAAATGAAGTACCAGGCAAGGTGCTGCATCCCTCATCAACAATGGGAGACACAGTAGTAGATGATGAATCCTCCGGATCTATGGCTGATGTAACTGGTGATATTGAAGGGAAGACAGGCGATTCACTGCCTTACCCCATTTTGCGGCCAATCATCATATCAAGATCAAGAGACTTTAAGCGAAGCCATGATCATAAAAGCCCATGTGTTCCTCCTACTATGCGTGATCGACCTCGGATAAGGCGGCCACCCTCGCCTGTGGTGCTTTCTGTCCCACGGGCTCCACGACCACCTCCGCCATCTCCTGTGAGTGACTCCAGAAAACACAGGGGATTTCCAACTGTTCGATCTGGTAGTTCTAGCCCAAGGCACTGGGGTATGAGAGGCTGGTTCCATGATGGAGCTAACTTGGACGAAGCTTGTTTACGCATGGATGGTGCTGAAGTTGTTTGGCCCTTTAGAAATAATAATAATATTTCAGGCCGCCCGTTGATTCAGTCTCTTCCTGCACCTCTGCTGCAAGATAGACTGATTGCTATTTCTCAACTAGCACGTGATCAGGAACATCCGGATGTTGCATTCCCCATTCAACCACCTGATTTACATAATTGTCCAATACGGAAGGCCTCTCTCTCTCTGATGCACAGCCTCGTGCATAACGAGATTGAATTTTTTTGCAAGAAGGTTGCTACAGAGAATATGGCTCGGAAGCCCTACATCAATTGGGCTGTCAAACGAGTTACACGGTCTCTCCAGGTGCTTTGGCCCAGATCCAGGACAAATATATTTGGTTCAGTTGCAAACGGTTTGTCCCTTCCAACAAGTGATGTGGACCTTGTGGTTTGTCTACCTCCAGTTAGGAACTTGGAACCCATTAAAGAAGCTGGGATATTGGAGGGCCGAAATGGTATTAAAGAGACATGCCTTCAGCATGCTGCCAGGTATCTTGTCAATCAGGATTGGGTTAAAAATGATTCTCTTAAGACGGTGGAAAATACAGCTATACCGATTATAATGCTTGTGGTGGAAGTTCCCAACGATCTAATTGCCTCTTCCGCCTCTAATGTGCAATCACCAAAAGAAGAGGCACCTCACAATACTGGTGAACCAGACAATAATGCACACTCCAGTGGGGTTGTTTTAGAAGAATCTGCAATGCCTAAGTGCCCACAGATAACTTATGATGCTACAAAGGATTCAGTATCAATTCGCATTGACATCAGTTTCAAATCTCCTTCTCATACAGGATTTCAAACTACCCAGCTGGTTAAAGATCTAACTGAGCAGTTTCCAGCGGCTACTCCCCTTGCTTTGGTACTGAAACAGTTCTTGGCAGATCGTAGTCTGGATCAGTCTTATTCTGGTGGCTTGAGTTCCTACTGTTTGGTACTACTGATAGTGCGTTTTCTCCAGCATGAGTATCATCTTGGCCGACCTATCAACCAAAACTTTGGAAACCTTCTGATGAATTTTCTTTATTTTTTTGGGAATGTTTTCGATCCTCGGCAAATGCGTATTTCAGTACAGGGAAGCGGTGTTTATATAAAGAGAGAAAGAGGTTGCAGCATCGACCCTATACACATTGATGATCCCCTTTTTCCGACTAATAATGTGGGACGCAATTGCTTCCGGATACATCAGTGTATCAAGGCATTTTCAGAAGCTTATTCTATTATGGAGACTGAGCTTGCTTCTCTTCCTAATGATGATGATGCTGAATCAAGTCCCCCATATAGACTGCTTCCTAAAATTATCCCGAGTATTGATCTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

1281

Amino Acids

141.61

Weight (kDa)

6.48

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 932 - 1029 1.1e-07 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1809, 3080
AarI CACCTGC 1 cut(s) 1393
Acc16I TGCGCA 1 cut(s) 62
Acc36I ACCTGC 2 cut(s) 145, 1393
AccB1I GGYRCC 1 cut(s) 3154
AccI GTMKAC 1 cut(s) 2914
AccII CGCG 1 cut(s) 746
AccIII TCCGGA 3 cut(s) 2141, 2635, 3696
AciI CCGC 9 cut(s) 746, 1435, 2212, 2311, 2363, 2551, 3123, 3362, 3600
AclWI GGATC 9 cut(s) 507, 1042, 1709, 2041, 2152, 2832, 3423, 3560, 3650
AcoI YGGCCR 3 cut(s) 2213, 2312, 3496
AcsI RAATTY 5 cut(s) 95, 233, 874, 2735, 3535
AcuI CTGAAG 6 cut(s) 161, 1065, 1065, 1101, 2529, 2978
AcvI CACGTG 1 cut(s) 2621
AfiI CCNNNNNNNGG 4 cut(s) 1094, 2211, 2841, 3379
AflII CTTAAG 1 cut(s) 3047
AhlI ACTAGT 1 cut(s) 1735
AjnI CCWGG 6 cut(s) 1030, 1336, 2080, 2820, 2840, 3005
AjuI GAANNNNNNNTTGG 4 cut(s) 1236, 1268, 3380, 3412
AleI CACNNNNGTG 1 cut(s) 3195
Alw21I GWGCWC 1 cut(s) 1558
Alw26I GTCTC 7 cut(s) 1609, 2109, 2232, 2571, 2820, 2978, 3740
AlwI GGATC 9 cut(s) 507, 1042, 1709, 2041, 2152, 2832, 3423, 3560, 3650
AlwNI CAGNNNCTG 4 cut(s) 1043, 1088, 1124, 1758
Ama87I CYCGRG 1 cut(s) 3823
Aor13HI TCCGGA 3 cut(s) 2141, 2635, 3696
ApeKI GCWGC 5 cut(s) 656, 2092, 2584, 3002, 3630
ApoI RAATTY 5 cut(s) 95, 233, 874, 2735, 3535
ArsI GACNNNNNNTTYG 6 cut(s) 80, 112, 1012, 1044, 2861, 2893
Asp700I GAANNNNTTC 4 cut(s) 236, 1251, 3392, 3560
AspLEI GCGC 1 cut(s) 63
AsuC2I CCSGG 1 cut(s) 1861
AsuHPI GGTGA 6 cut(s) 827, 1424, 1477, 2177, 3132, 3182
AsuII TTCGAA 1 cut(s) 1549
AvaI CYCGRG 1 cut(s) 3823
AvaII GGWCC 5 cut(s) 902, 1264, 1578, 1783, 2899
AxyI CCTNAGG 2 cut(s) 1089, 1125
BaeGI GKGCMC 1 cut(s) 3236
BaeI ACNNNNGTAYC 2 cut(s) 3692, 3725
BanI GGYRCC 1 cut(s) 3154
BanII GRGCYC 1 cut(s) 2350
BarI GAAGNNNNNNTAC 2 cut(s) 2475, 2507
BauI CACGAG 1 cut(s) 2717
BbrPI CACGTG 1 cut(s) 2621
BbsI GAAGAC 5 cut(s) 557, 987, 1071, 1107, 2186
Bbv12I GWGCWC 1 cut(s) 1558
BbvI GCAGC 5 cut(s) 643, 2079, 2571, 2989, 3642
BccI CCATC 6 cut(s) 941, 1055, 1155, 2374, 2459, 2495
BceAI ACGGC 1 cut(s) 390
BcgI CGANNNNNNTGC 4 cut(s) 3064, 3095, 3098, 3129
BciT130I CCWGG 6 cut(s) 1032, 1338, 2082, 2822, 2842, 3007
BciVI GTATCC 1 cut(s) 3693
BclI TGATCA 2 cut(s) 2257, 2623
BcnI CCSGG 1 cut(s) 1861
BcoDI GTCTC 7 cut(s) 1609, 2109, 2232, 2571, 2820, 2978, 3740
BcuI ACTAGT 1 cut(s) 1735
BfaI CTAG 7 cut(s) 393, 402, 852, 1296, 1736, 2427, 2615
BfmI CTRYAG 2 cut(s) 241, 2757
BfrI CTTAAG 1 cut(s) 3047
BfuAI ACCTGC 2 cut(s) 145, 1393
BfuI GTATCC 1 cut(s) 3693
BglII AGATCT 1 cut(s) 3340
BisI GCNGC 9 cut(s) 657, 2093, 2213, 2312, 2551, 2585, 3003, 3363, 3631
BlsI GCNGC 9 cut(s) 658, 2094, 2214, 2313, 2552, 2586, 3004, 3364, 3632
BmcAI AGTACT 1 cut(s) 1817
Bme1390I CCNGG 7 cut(s) 1032, 1338, 1861, 2082, 2822, 2842, 3007
Bme18I GGWCC 5 cut(s) 902, 1264, 1578, 1783, 2899
BmeT110I CYCGRG 1 cut(s) 3823
BmiI GGNNCC 8 cut(s) 8, 958, 1538, 1569, 2349, 2459, 2937, 3156
BmrFI CCNGG 7 cut(s) 1032, 1338, 1861, 2082, 2822, 2842, 3007
BmrI ACTGGG 1 cut(s) 2449
BmsI GCATC 8 cut(s) 142, 910, 1411, 2104, 2697, 3241, 3642, 3769
BmuI ACTGGG 1 cut(s) 2449
BpiI GAAGAC 5 cut(s) 557, 987, 1071, 1107, 2186
BplI GAGNNNNNCTC 2 cut(s) 288, 320
BpmI CTGGAG 5 cut(s) 2368, 2804, 2904, 3178, 3461
Bpu10I CCTNAGC 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 1549
BpuEI CTTGAG 2 cut(s) 724, 3457
BpuMI CCSGG 1 cut(s) 1861
Bsa29I ATCGAT 1 cut(s) 1396
BsaAI YACGTR 1 cut(s) 2621
BsaBI GATNNNNATC 2 cut(s) 564, 1809
BsaI GGTCTC 1 cut(s) 2820
BsaJI CCNNGG 5 cut(s) 495, 2300, 2342, 2432, 3569
BsaWI WCCGGW 4 cut(s) 1575, 2141, 2635, 3696
BsaXI ACNNNNNCTCC 6 cut(s) 1285, 1315, 2106, 2136, 2802, 2832
Bsc4I CCNNNNNNNGG 4 cut(s) 1094, 2211, 2841, 3379
Bse21I CCTNAGG 2 cut(s) 1089, 1125
Bse3DI GCAATG 1 cut(s) 3228
Bse8I GATNNNNATC 2 cut(s) 564, 1809
BseAI TCCGGA 3 cut(s) 2141, 2635, 3696
BseBI CCWGG 6 cut(s) 1032, 1338, 2082, 2822, 2842, 3007
BseCI ATCGAT 1 cut(s) 1396
BseDI CCNNGG 5 cut(s) 495, 2300, 2342, 2432, 3569
BseGI GGATG 8 cut(s) 1240, 1414, 1747, 1777, 2095, 2506, 2632, 2644
BseJI GATNNNNATC 2 cut(s) 564, 1809
BseLI CCNNNNNNNGG 4 cut(s) 1094, 2211, 2841, 3379
BseMI GCAATG 1 cut(s) 3228
BseMII CTCAG 6 cut(s) 517, 1080, 1116, 1758, 3339, 3741
BseSI GKGCMC 1 cut(s) 3236
BseXI GCAGC 5 cut(s) 643, 2079, 2571, 2989, 3642
BseYI CCCAGC 3 cut(s) 115, 2951, 3327
BsgI GTGCAG 3 cut(s) 310, 1722, 2559
Bsh1236I CGCG 1 cut(s) 746
BshNI GGYRCC 1 cut(s) 3154
BshVI ATCGAT 1 cut(s) 1396
BsiHKAI GWGCWC 1 cut(s) 1558
BsiHKCI CYCGRG 1 cut(s) 3823
BsiSI CCGG 5 cut(s) 1576, 1860, 2142, 2636, 3697
BslFI GGGAC 5 cut(s) 1876, 2324, 2863, 3696, 3777
BslI CCNNNNNNNGG 4 cut(s) 1094, 2211, 2841, 3379
BsmAI GTCTC 7 cut(s) 1609, 2109, 2232, 2571, 2820, 2978, 3740
BsmFI GGGAC 5 cut(s) 1876, 2324, 2863, 3696, 3777
BsmI GAATGC 3 cut(s) 715, 1322, 2645
Bso31I GGTCTC 1 cut(s) 2820
BsoBI CYCGRG 1 cut(s) 3823
Bsp119I TTCGAA 1 cut(s) 1549
Bsp1286I GDGCHC 3 cut(s) 1558, 2350, 3236
Bsp13I TCCGGA 3 cut(s) 2141, 2635, 3696
Bsp1407I TGTACA 1 cut(s) 464
BspACI CCGC 9 cut(s) 746, 1435, 2212, 2311, 2363, 2551, 3123, 3362, 3600
BspCNI CTCAG 6 cut(s) 516, 1081, 1117, 1759, 3340, 3742
BspDI ATCGAT 1 cut(s) 1396
BspEI TCCGGA 3 cut(s) 2141, 2635, 3696
BspFNI CGCG 1 cut(s) 746
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 8 cut(s) 8, 958, 1538, 1569, 2349, 2459, 2937, 3156
BspMI ACCTGC 2 cut(s) 145, 1393
BspPI GGATC 9 cut(s) 507, 1042, 1709, 2041, 2152, 2832, 3423, 3560, 3650
BspT104I TTCGAA 1 cut(s) 1549
BspT107I GGYRCC 1 cut(s) 3154
BspTI CTTAAG 1 cut(s) 3047
BspTNI GGTCTC 1 cut(s) 2820
BsrDI GCAATG 1 cut(s) 3228
BsrGI TGTACA 1 cut(s) 464
BssECI CCNNGG 5 cut(s) 495, 2300, 2342, 2432, 3569
BssSI CACGAG 1 cut(s) 2717
BssT1I CCWWGG 2 cut(s) 495, 2432
Bst2BI CACGAG 1 cut(s) 2717
Bst2UI CCWGG 6 cut(s) 1032, 1338, 2082, 2822, 2842, 3007
Bst6I CTCTTC 4 cut(s) 2574, 3124, 3144, 3768
BstAFI CTTAAG 1 cut(s) 3047
BstAUI TGTACA 1 cut(s) 464
BstBAI YACGTR 1 cut(s) 2621
BstBI TTCGAA 1 cut(s) 1549
BstC8I GCNNGC 4 cut(s) 427, 1179, 3000, 3756
BstDSI CCRYGG 1 cut(s) 2342
BstENI CCTNNNNNAGG 1 cut(s) 1092
BstF5I GGATG 8 cut(s) 1240, 1414, 1747, 1777, 2095, 2506, 2632, 2644
BstFNI CGCG 1 cut(s) 746
BstHHI GCGC 1 cut(s) 63
BstMAI GTCTC 7 cut(s) 1609, 2109, 2232, 2571, 2820, 2978, 3740
BstMWI GCNNNNNNNGC 5 cut(s) 146, 1174, 2320, 2329, 3122
BstNI CCWGG 6 cut(s) 1032, 1338, 2082, 2822, 2842, 3007
BstNSI RCATGY 3 cut(s) 623, 2991, 3002
BstSCI CCNGG 7 cut(s) 1030, 1336, 1859, 2080, 2820, 2840, 3005
BstSFI CTRYAG 2 cut(s) 241, 2757
BstSLI GKGCMC 1 cut(s) 3236
BstUI CGCG 1 cut(s) 746
BstV1I GCAGC 5 cut(s) 643, 2079, 2571, 2989, 3642
BstV2I GAAGAC 5 cut(s) 557, 987, 1071, 1107, 2186
BstX2I RGATCY 5 cut(s) 499, 2033, 2144, 2837, 3340
BstXI CCANNNNNNTGG 4 cut(s) 1274, 2051, 2439, 3518
BstYI RGATCY 5 cut(s) 499, 2033, 2144, 2837, 3340
Bsu15I ATCGAT 1 cut(s) 1396
Bsu36I CCTNAGG 2 cut(s) 1089, 1125
BsuI GTATCC 1 cut(s) 3693
BsuTUI ATCGAT 1 cut(s) 1396
BtgI CCRYGG 1 cut(s) 2342
BtsCI GGATG 8 cut(s) 1240, 1414, 1747, 1777, 2095, 2506, 2632, 2644
BtsI GCAGTG 3 cut(s) 789, 1514, 2192
BveI ACCTGC 2 cut(s) 145, 1393
Cac8I GCNNGC 4 cut(s) 427, 1179, 3000, 3756
CaiI CAGNNNCTG 4 cut(s) 1043, 1088, 1124, 1758
CciI TCATGA 1 cut(s) 1068
CfoI GCGC 1 cut(s) 63
ClaI ATCGAT 1 cut(s) 1396
CseI GACGC 2 cut(s) 562, 3693
CspCI CAANNNNNGTGG 4 cut(s) 63, 98, 2877, 2912
EaeI YGGCCR 3 cut(s) 2213, 2312, 3496
Eam1104I CTCTTC 4 cut(s) 2574, 3124, 3144, 3768
EarI CTCTTC 4 cut(s) 2574, 3124, 3144, 3768
EciI GGCGGA 2 cut(s) 2352, 3112
Eco130I CCWWGG 2 cut(s) 495, 2432
Eco147I AGGCCT 1 cut(s) 2693
Eco24I GRGCYC 1 cut(s) 2350
Eco31I GGTCTC 1 cut(s) 2820
Eco32I GATATC 3 cut(s) 184, 198, 1764
Eco47I GGWCC 5 cut(s) 902, 1264, 1578, 1783, 2899
Eco57I CTGAAG 6 cut(s) 161, 1065, 1065, 1101, 2529, 2978
Eco72I CACGTG 1 cut(s) 2621
Eco81I CCTNAGG 2 cut(s) 1089, 1125
Eco88I CYCGRG 1 cut(s) 3823
EcoNI CCTNNNNNAGG 1 cut(s) 1092
EcoRII CCWGG 6 cut(s) 1030, 1336, 2080, 2820, 2840, 3005
EcoRV GATATC 3 cut(s) 184, 198, 1764
EcoT14I CCWWGG 2 cut(s) 495, 2432
EcoT22I ATGCAT 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 2350
ErhI CCWWGG 2 cut(s) 495, 2432
FaqI GGGAC 5 cut(s) 1876, 2324, 2863, 3696, 3777
FauNDI CATATG 1 cut(s) 1729
FbaI TGATCA 2 cut(s) 2257, 2623
FblI GTMKAC 1 cut(s) 2914
Fnu4HI GCNGC 9 cut(s) 657, 2093, 2213, 2312, 2551, 2585, 3003, 3363, 3631
FokI GGATG 8 cut(s) 1247, 1421, 1754, 1784, 2082, 2513, 2619, 2651
FriOI GRGCYC 1 cut(s) 2350
Fsp4HI GCNGC 9 cut(s) 657, 2093, 2213, 2312, 2551, 2585, 3003, 3363, 3631
FspBI CTAG 7 cut(s) 393, 402, 852, 1296, 1736, 2427, 2615
FspI TGCGCA 1 cut(s) 62
GlaI GCGC 1 cut(s) 62
GluI GCNGC 9 cut(s) 657, 2093, 2213, 2312, 2551, 2585, 3003, 3363, 3631
GsaI CCCAGC 3 cut(s) 119, 2955, 3331
GsuI CTGGAG 5 cut(s) 2368, 2804, 2904, 3178, 3461
HapII CCGG 5 cut(s) 1576, 1860, 2142, 2636, 3697
HgaI GACGC 2 cut(s) 562, 3693
HhaI GCGC 1 cut(s) 63
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 1369
HindII GTYRAC 1 cut(s) 1369
HindIII AAGCTT 3 cut(s) 750, 2484, 3729
HpaI GTTAAC 1 cut(s) 1369
HpaII CCGG 5 cut(s) 1576, 1860, 2142, 2636, 3697
HphI GGTGA 6 cut(s) 827, 1424, 1477, 2177, 3132, 3182
Hpy166II GTNNAC 7 cut(s) 1240, 1369, 1620, 2492, 2899, 2915, 3173
Hpy8I GTNNAC 7 cut(s) 1240, 1369, 1620, 2492, 2899, 2915, 3173
HpyCH4IV ACGT 2 cut(s) 488, 2620
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 1174, 2320, 2329, 3122
HpySE526I ACGT 2 cut(s) 488, 2620
HspAI GCGC 1 cut(s) 61
Kpn2I TCCGGA 3 cut(s) 2141, 2635, 3696
Ksp22I TGATCA 2 cut(s) 2257, 2623
KspAI GTTAAC 1 cut(s) 1369
LmnI GCTCC 5 cut(s) 12, 25, 1573, 2353, 2468
Lsp1109I GCAGC 5 cut(s) 643, 2079, 2571, 2989, 3642
LweI GCATC 8 cut(s) 142, 910, 1411, 2104, 2697, 3241, 3642, 3769
MaeI CTAG 7 cut(s) 393, 402, 852, 1296, 1736, 2427, 2615
MaeII ACGT 2 cut(s) 488, 2620
MaeIII GTNAC 6 cut(s) 857, 986, 1748, 2158, 2378, 2806
MfeI CAATTG 2 cut(s) 2788, 3688
MflI RGATCY 5 cut(s) 499, 2033, 2144, 2837, 3340
MhlI GDGCHC 3 cut(s) 1558, 2350, 3236
MlyI GAGTC 5 cut(s) 551, 599, 1085, 1501, 2375
MmeI TCCRAC 4 cut(s) 1742, 2429, 2909, 3692
Mph1103I ATGCAT 1 cut(s) 157
MroI TCCGGA 3 cut(s) 2141, 2635, 3696
MroXI GAANNNNTTC 4 cut(s) 236, 1251, 3392, 3560
MseI TTAA 9 cut(s) 159, 1185, 1368, 2244, 2943, 2979, 3033, 3048, 3336
MslI CAYNNNNRTG 6 cut(s) 20, 191, 1407, 2637, 3195, 3651
MspA1I CMGCKG 3 cut(s) 656, 3331, 3362
MspCI CTTAAG 1 cut(s) 3047
MspI CCGG 5 cut(s) 1576, 1860, 2142, 2636, 3697
MspR9I CCNGG 7 cut(s) 1032, 1338, 1861, 2082, 2822, 2842, 3007
MunI CAATTG 2 cut(s) 2788, 3688
Mva1269I GAATGC 3 cut(s) 715, 1322, 2645
MvaI CCWGG 6 cut(s) 1032, 1338, 2082, 2822, 2842, 3007
MvnI CGCG 1 cut(s) 746
MwoI GCNNNNNNNGC 5 cut(s) 146, 1174, 2320, 2329, 3122
NciI CCSGG 1 cut(s) 1861
NdeI CATATG 1 cut(s) 1729
NlaIV GGNNCC 8 cut(s) 8, 958, 1538, 1569, 2349, 2459, 2937, 3156
NmeAIII GCCGAG 2 cut(s) 1454, 3550
NmuCI GTSAC 2 cut(s) 1748, 2378
NsbI TGCGCA 1 cut(s) 62
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 3 cut(s) 623, 2991, 3002
NspV TTCGAA 1 cut(s) 1549
OliI CACNNNNGTG 1 cut(s) 3195
PaeI GCATGC 1 cut(s) 3002
PagI TCATGA 1 cut(s) 1068
PaqCI CACCTGC 1 cut(s) 1393
PceI AGGCCT 1 cut(s) 2693
PcsI WCGNNNNNNNCGW 4 cut(s) 1555, 2350, 2724, 3577
PctI GAATGC 3 cut(s) 715, 1322, 2645
PdmI GAANNNNTTC 4 cut(s) 236, 1251, 3392, 3560
PfoI TCCNGGA 2 cut(s) 1030, 2840
PkrI GCNGC 9 cut(s) 658, 2094, 2214, 2313, 2552, 2586, 3004, 3364, 3632
PleI GAGTC 5 cut(s) 550, 598, 1084, 1501, 2375
PmaCI CACGTG 1 cut(s) 2621
PmlI CACGTG 1 cut(s) 2621
PpsI GAGTC 5 cut(s) 550, 598, 1084, 1501, 2375
Ppu21I YACGTR 1 cut(s) 2621
PsiI TTATAA 2 cut(s) 1809, 3080
Psp6I CCWGG 6 cut(s) 1030, 1336, 2080, 2820, 2840, 3005
PspCI CACGTG 1 cut(s) 2621
PspFI CCCAGC 3 cut(s) 115, 2951, 3327
PspGI CCWGG 6 cut(s) 1030, 1336, 2080, 2820, 2840, 3005
PspN4I GGNNCC 8 cut(s) 8, 958, 1538, 1569, 2349, 2459, 2937, 3156
PstNI CAGNNNCTG 4 cut(s) 1043, 1088, 1124, 1758
PsuI RGATCY 5 cut(s) 499, 2033, 2144, 2837, 3340
PvuII CAGCTG 2 cut(s) 656, 3331
RseI CAYNNNNRTG 6 cut(s) 20, 191, 1407, 2637, 3195, 3651
SaqAI TTAA 9 cut(s) 159, 1185, 1368, 2244, 2943, 2979, 3033, 3048, 3336
SatI GCNGC 9 cut(s) 657, 2093, 2213, 2312, 2551, 2585, 3003, 3363, 3631
ScaI AGTACT 1 cut(s) 1817
SchI GAGTC 5 cut(s) 551, 599, 1085, 1501, 2375
ScrFI CCNGG 7 cut(s) 1032, 1338, 1861, 2082, 2822, 2842, 3007
SduI GDGCHC 3 cut(s) 1558, 2350, 3236
SfaNI GCATC 8 cut(s) 142, 910, 1411, 2104, 2697, 3241, 3642, 3769
SfcI CTRYAG 2 cut(s) 241, 2757
SfuI TTCGAA 1 cut(s) 1549
SinI GGWCC 5 cut(s) 902, 1264, 1578, 1783, 2899
SmiMI CAYNNNNRTG 6 cut(s) 20, 191, 1407, 2637, 3195, 3651
SmlI CTYRAG 3 cut(s) 703, 3047, 3436
SmoI CTYRAG 3 cut(s) 703, 3047, 3436
SpeI ACTAGT 1 cut(s) 1735
SphI GCATGC 1 cut(s) 3002
SseBI AGGCCT 1 cut(s) 2693
SsiI CCGC 9 cut(s) 746, 1435, 2212, 2311, 2363, 2551, 3123, 3362, 3600
SspI AATATT 2 cut(s) 1006, 2542
SspMI CTAG 7 cut(s) 393, 402, 852, 1296, 1736, 2427, 2615
StuI AGGCCT 1 cut(s) 2693
StyD4I CCNGG 7 cut(s) 1030, 1336, 1859, 2080, 2820, 2840, 3005
StyI CCWWGG 2 cut(s) 495, 2432
TaiI ACGT 2 cut(s) 491, 2623
TaqII GACCGA 1 cut(s) 1403
TatI WGTACW 4 cut(s) 464, 1815, 1836, 3589
TauI GCSGC 4 cut(s) 2215, 2314, 2553, 3365
Tru1I TTAA 9 cut(s) 159, 1185, 1368, 2244, 2943, 2979, 3033, 3048, 3336
Tru9I TTAA 9 cut(s) 159, 1185, 1368, 2244, 2943, 2979, 3033, 3048, 3336
TseFI GTSAC 2 cut(s) 1748, 2378
TseI GCWGC 5 cut(s) 656, 2092, 2584, 3002, 3630
Tsp45I GTSAC 2 cut(s) 1748, 2378
TspGWI ACGGA 2 cut(s) 1560, 2701
Vha464I CTTAAG 1 cut(s) 3047
VpaK11BI GGWCC 5 cut(s) 902, 1264, 1578, 1783, 2899
XagI CCTNNNNNAGG 1 cut(s) 1092
XapI RAATTY 5 cut(s) 95, 233, 874, 2735, 3535
XceI RCATGY 3 cut(s) 623, 2991, 3002
XcmI CCANNNNNNNNNTGG 1 cut(s) 1793
XmiI GTMKAC 1 cut(s) 2914
XmnI GAANNNNTTC 4 cut(s) 236, 1251, 3392, 3560
XspI CTAG 7 cut(s) 393, 402, 852, 1296, 1736, 2427, 2615
ZrmI AGTACT 1 cut(s) 1817
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.