Rw1G018480

Nucleotidyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003190
Physical Location & Seq
Reverse (-)
40791341 .. 40801295
9955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018480.1

Sequence Viewer

Length: 4662 bp
ATGTCGAAACCAAACCCTAAATCCCCTTCCAATTCCCGCATAGCTCAGAACCAACTCATCGACTCCCTCACCTCCCACATCTCCCTCTACAATTCCCACTCCCATTCCTCAGACCCCAAGCCCAACCCTAACCCTAGGTCCTCCATCCTCAAATGGTTCTCTTCTCTCTCCCTCCACCACCGCCAAGCTCACCTCACCGCCGTCGATCCCAAATTCGTCCGCCTCCTCCTCCAAATGCTCCGCAAGCTCCGCACGCAGGGCCACGGCTCCTTCATCATCCTCCCCGACCTCCCCTCCGGCGACCTCCCCACGCTCTGCTTCAAGAGGTCCGGCGGGCTTTTGTCTAGGGTTGCCGAGTCGAACGAGTCGGAGAAGATGGTGTTCGAGTCGACTCGGTTGTTTTCGTCGAGGGAGGGAGAGAAGGTCGAGGAGTGTTCGTGTTCGTTGGTTGAGAATTTAGATAGGTTTGTGGAGGCTATGGATGATATATCAAATGGGGGATTCCTGAGAGGTGAAGACAGTGATTTGGGGTCGGATTGGGTGGAATTGAAGTGGTTGAAGGACAAAGGGTATTACAGTATGGAAGCTTTCGTGGCGAACCGGTTAGAGGTTGCTCTGAGGCTTGCTTGGCTGAATTCGAATAATGCAAGGAAAAGAGGGGTGAAGTTGAAAGAGAAGATCAGCGCCGCCGGCGTGGCGGCCAATGTGTATTGGAGGAAGAAAGGGTGTGTGGACTGGTGGGGGAATTTGGATGATGCGACGCGGAGCAATGTAGTGACTTCAGTGTTGGGGAAAGCAGCAAAACCGTTGATTCATGAGATTCTGAAGGGAACAAGTAGTGGGGTAGAGGATGAAATGTGGCTCGCCAACACAGGAATGGAGCAACCATTGAGGTACAACCATAATGTATCTATGCGCAAGACTGTTCCAAAACTTGTGGCTGACACAGAATTTGGGTCAAGCATTATCCCAGCTTCTCTTTCTGGGAAACCTGCTTCCTTAGCCGATGCTTTTAATAATCTCTTTGTGCTTCAGGATATCATAACGATGATATCATTATGTCGCCATAATGAATATGATAAAGGGAAATTCTTCTATACCACATTGAGTTCAATTTCTACCATTTCTGATTTTATACTAAGAAAAATACGAGGATTTCTCATGGTTCTTTTGCTTGACTGCACAAAACTTGAACTTTTAGCAGAGGGAAATGAAAAATGCTTACCTAAGAAACCCAAAGCAAAGCCTAGTGCTTCTAGCCGTAAAAGTAAGGGGAGGTCCCACAATATGAAAAGGCCAAATCCTGTTCCAGGGTCATGTACAGATGAATTTTTGTGTGAAACATCTGCTAAGGATCTTAGTACATTGGCTTATAAAGAGAAGGCAGGTTCAGTGGAGTCCAAGACGACGCATACTATACATCAAGAGAAGGAGATTTCCAGAGAATCGTCATCATCAAAAGATGAAATGGAACATGCACAAGCATTGGCTGTTGGAAAAGTGCAGACAGCTGCGAGGAAGGGTAGGAAAGGAAAAGGCAAAAAGAAAATAACTGGCTTGAGGAGTGCTAATGATATGGAGAAATTTGATAGATCATTCATGGAAGCTTCTTCTTCTTGTTCAGTCATTCTTGAAGATGACACAGCAAAGTCTGATCAGGTTTCTGGTGAAACAGCCTACCAGAACATCGGATGTGATAATTCAGCTAGTTGTAACATCCCTTTGTCAAATTCAATTCCTTCTGGTTCTGCGAATGGACCTCTTAAAGATGAAGATGCCACCAAAAGCATTCAAGAGAATTATGGTATTGGTTCTAGTGCCAGTTTCTGTCATAAGGTTCTGGAAAGTTATCAGTGTTTAAATAATAATACTGAAATCCAAACAAAATGTTCTGGATCAGAAACTGAAGCCTGTAAGGTAGATGGAAACTTGATAAAGTCTCCAGTGCCTGAGGTAGATGATAAAGTTTCCCATCATAAAGATATTGACTTGCAGGACAAGCGTGCTGTTAAGTCAGGTGCGAAAGAAGTTTTGCCAGCCAAAGAAATAAGAGCTTCTGATGTAAACGAGGAAGCTGTTCTGTTGCAGGACCAAGAGAGTGGAAATAATATATATCACACTAGGGCTCCAAGTTCTTTTGATTGCCCTCCTTATGAGTGGCCGGCTGTAGCTTGTGCCTATTTTCCACCTGTTAACTCACATCTCCCACCTGCCACCGATCGATTGCATCTGGATGTTGGTCATAACTGGCAGAATCACCGCCAGTCTTTTCTACCCACAATACATCGGGTGAGAAACTCGGCAATTGAAGGTGGGTGTAATCCAGTTCTGACTCGACCATTGCCAATGAGTATAGATTGGCCCCCAATGGTTCGAAGTGCTCGTAGAGTGGCTCCATACCGGACCTGTAATTATGATTCTGGGTTTGTCTCAAAAAAGCCGTGTACTTATCCACAGGGTTTTGCACACACTGTCCAAATAAATGCAACAACCATGGATGATGAAAGGAAGTATTCTTGGGATTGCGCAGACCTACCCGATCCAACAAAGGCATATGAACTAGTGGATGAATGTGACAGTCAATGGATATCTGAGGATGAAGTTGAGGTGCAAGCATTTTCTGGGGCAGATTATAATCAATACTTTGGTGGTGGTGTGATGTACTGGAATCCTTCTGATCATACCGGGACAGTTTTCTCTCGCCCTCCTTCCCTTAGTTCTGATGATAGCTCATGGGCTTGGCGTGAAGCTGACATAAATAGAGCTGTTGATGATATGGTTGCATTTTCTTCTCCGTACAGTACAAATGGTTTGACTTCACCTACTGCTTCCTTCTGTTCTCCTTTTGATCCTTTGGGATCAGGAAACCAGCCTCTTGGATATGTTATGTCAGGAAATGAAGTACCAGGCAAGGTGCTGCATCCCTCATCAACAATGGGAGACACAGTAATAGATGAGGAATCGTCTGGATCTTTGGCTGACGTAACTGGTGATGTTGAAGGGAAGACAGGCGATTCATTGCCTTACCCCATGTTGCGGCCAATCATCATATCAAGATCAAGAGACTTTAAGCGCAGCCATGATCGTAAAAGCCCATGTGTTCCTCCTACTATGCGCGATCGACCTAGGATAAAGCGGCCACCGTCGCCGGTGGTGCTTTCTGTCCCACGCGCTCCACGACCACCTCCACCATCTTCTGTGAGTGACTCGAGGAAACACAGGGGATTTCCAACTGTTCGTTCTGGTAGCTCTAGCCCAAGGCACTGGGGTATGAGAGGTTGGTTCCATGATGGAGCTAACTTGGAGGAAGCTTGTTTACGCATGGATGGTGCTGAAGTTGTTTGGCCATTTAGAAATAATATTTCAGGCCCCCCATTGATTCAGCCTCTTCCTGCACCTCTGTTGCAGGATCGACTGATTGCTATTTCTCAACTAGCACGTGATCAGGAACATCCGGATGTTGCATTTCCCCTTCAGCCGCCTGAGTTACATAACTGTCCAACACGGAAGGCAAATCTCTCTCTGATGCACAGCCTTGTGCATGATGAGATTGAATTTTTTTGCAAGAAGGTTGCTACAGAGAATATGTCTCGGAAGCCCTACATCAATTGGGCTGTCAAACGAGTTACACGGTCTCTCCAGGTGCTTTGGCCCAGATCCAGGACAAATATATTTGGTTCAGTTGCAAACGGTTTGTCCCTTCCAACTAGTGATGTGGACCTTGTGGTTTCTCTACCTCCTGTTAGGAATTTGGAACCCATTAAAGAAGCTGGGATATTGGAGGGTCGAAATGGTATTAAAGAGACATGCCTTCAGCATGCTGCCAGGTATCTCGTCAATCAGGATTGGGTTAAAAATGATTCTCTTAAGACCGTGGAAAATACAGCTATACCGATTATAATGCTTGTGGTGGAAGTTCCCAATGATCTAATTGCCTCGTCTTCGTCTAACGTGCAATCACCAAAAGAAGAGGCACCACACAGTACTGGTGAACCAGACTGTAATGTGCACTCCAGTGGGGTTATTTTAGAAGAATCTGCTATGCCTAAGTGCCCCCAGATAACTTGTGATGCTACAAAGGATTCAGTATCAATTCGCATTGACATCAGTTTCAAATCTCCATCTCATACAGGATTTCAAACTACACAGCTGGTTAAAGATTTAACTGAGCAGTTTCCAGCAGCTACACCCCTTGCTTTGGTACTGAAACAGTTCTTGGCGGATCGTAGTCTGGATCAGTCTTATTCTGGTGGCTTGAGTTCCTACTGCTTGGTACTACTAATTGTGCGTTTTCTCCAGCATGAGTATCATCTTAGCCGACCTATCAACCAAAACTTTGGAAACCTTCTGATGAATTTTCTTTATTTTTTTGGGAATGTTTTTGATCCTCGGCAAATGCGTATTTCCGTACAGGGAAGTGGTGTTTATATAAAGAGGGAAAGAGGTTGCAGCATCGACCCCATACACATTGATGATCCCCTTTATCCGACTAATAATGTCGGACGCAATTGCTTTCGGATACATCAGTGTATCAAGGCGTTTTCAGAAGCTTATTCTATTTTGGAGAATGAGCTTGCTTCTCTTCCTAATGATGATGATGCATCTTCAAGTCCCCCATATAGACTGCTTCCTAAAATTATCCCGAGTATTGATCTAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

1553

Amino Acids

171.98

Weight (kDa)

6.76

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 1205 - 1301 1.9e-07 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1374, 2634, 3899
AarI CACCTGC 1 cut(s) 2218
Acc16I TGCGCA 2 cut(s) 917, 2527
Acc36I ACCTGC 3 cut(s) 1000, 1376, 2218
AccB1I GGYRCC 1 cut(s) 3973
AccI GTMKAC 1 cut(s) 389
AccII CGCG 3 cut(s) 763, 3117, 3171
AccIII TCCGGA 1 cut(s) 3454
AcoI YGGCCR 5 cut(s) 699, 2159, 3038, 3137, 3344
AcuI CTGAAG 7 cut(s) 765, 845, 1016, 1926, 3354, 3458, 3797
AcvI CACGTG 1 cut(s) 3440
AfiI CCNNNNNNNGG 6 cut(s) 256, 607, 1310, 3036, 3660, 4198
AflII CTTAAG 1 cut(s) 3866
AgeI ACCGGT 1 cut(s) 600
AhlI ACTAGT 2 cut(s) 2560, 3707
AjnI CCWGG 5 cut(s) 1309, 2905, 3639, 3659, 3824
AjuI GAANNNNNNNTTGG 4 cut(s) 3223, 3255, 4199, 4231
AleI CACNNNNGTG 1 cut(s) 4014
Alw21I GWGCWC 2 cut(s) 2383, 4011
Alw26I GTCTC 7 cut(s) 1944, 2434, 2934, 3057, 3594, 3639, 3797
Alw44I GTGCAC 1 cut(s) 4007
AlwNI CAGNNNCTG 3 cut(s) 1827, 1904, 1949
Ama87I CYCGRG 2 cut(s) 3208, 4642
Aor13HI TCCGGA 1 cut(s) 3454
ApaLI GTGCAC 1 cut(s) 4007
ApeKI GCWGC 7 cut(s) 797, 1511, 2917, 3075, 3821, 4181, 4449
ArsI GACNNNNNNTTYG 4 cut(s) 935, 967, 3680, 3712
AsiGI ACCGGT 1 cut(s) 600
Asp700I GAANNNNTTC 3 cut(s) 1091, 2076, 4211
AspA2I CCTAGG 2 cut(s) 134, 3125
AspLEI GCGC 6 cut(s) 686, 918, 2528, 3075, 3117, 3173
AsuC2I CCSGG 1 cut(s) 2686
AsuII TTCGAA 2 cut(s) 638, 2374
AvaI CYCGRG 2 cut(s) 3208, 4642
AvaII GGWCC 7 cut(s) 138, 327, 1278, 1757, 2089, 2403, 3718
AvrII CCTAGG 2 cut(s) 134, 3125
AxyI CCTNAGG 1 cut(s) 1950
BaeGI GKGCMC 2 cut(s) 4011, 4055
BaeI ACNNNNGTAYC 2 cut(s) 4511, 4544
BalI TGGCCA 1 cut(s) 3346
BanI GGYRCC 1 cut(s) 3973
BanII GRGCYC 1 cut(s) 2128
BarI GAAGNNNNNNTAC 2 cut(s) 3300, 3332
BbrPI CACGTG 1 cut(s) 3440
BbsI GAAGAC 3 cut(s) 522, 3011, 3933
Bbv12I GWGCWC 2 cut(s) 2383, 4011
BbvI GCAGC 7 cut(s) 809, 1498, 2904, 3087, 3808, 4193, 4461
BccI CCATC 8 cut(s) 152, 370, 1916, 1980, 3199, 3284, 3320, 4129
BceAI ACGGC 4 cut(s) 185, 280, 1245, 2425
BcgI CGANNNNNNTGC 2 cut(s) 3883, 3917
BciT130I CCWGG 5 cut(s) 1311, 2907, 3641, 3661, 3826
BciVI GTATCC 1 cut(s) 4512
BclI TGATCA 3 cut(s) 1654, 2677, 3442
BcnI CCSGG 1 cut(s) 2686
BcoDI GTCTC 7 cut(s) 1944, 2434, 2934, 3057, 3594, 3639, 3797
BcuI ACTAGT 2 cut(s) 2560, 3707
BfmI CTRYAG 2 cut(s) 2166, 3576
BfoI RGCGCY 1 cut(s) 687
BfrI CTTAAG 1 cut(s) 3866
BfuAI ACCTGC 3 cut(s) 1000, 1376, 2218
BfuI GTATCC 1 cut(s) 4512
BglI GCCNNNNNGGC 1 cut(s) 695
BlnI CCTAGG 2 cut(s) 134, 3125
BmcAI AGTACT 1 cut(s) 3985
Bme1390I CCNGG 6 cut(s) 1311, 2686, 2907, 3641, 3661, 3826
Bme18I GGWCC 7 cut(s) 138, 327, 1278, 1757, 2089, 2403, 3718
BmeT110I CYCGRG 2 cut(s) 3208, 4642
BmiI GGNNCC 9 cut(s) 268, 1280, 2127, 2363, 2394, 3284, 3370, 3756, 3975
BmrFI CCNGG 6 cut(s) 1311, 2686, 2907, 3641, 3661, 3826
BmrI ACTGGG 1 cut(s) 3274
BmuI ACTGGG 1 cut(s) 3274
BpiI GAAGAC 3 cut(s) 522, 3011, 3933
BplI GAGNNNNNCTC 2 cut(s) 1143, 1175
BpmI CTGGAG 4 cut(s) 1926, 3623, 3997, 4280
Bpu10I CCTNAGC 2 cut(s) 1000, 1350
Bpu14I TTCGAA 2 cut(s) 638, 2374
BpuEI CTTGAG 2 cut(s) 1579, 4276
BpuMI CCSGG 1 cut(s) 2686
Bsa29I ATCGAT 1 cut(s) 2221
BsaAI YACGTR 1 cut(s) 3440
BsaBI GATNNNNATC 1 cut(s) 2634
BsaI GGTCTC 1 cut(s) 3639
BsaJI CCNNGG 8 cut(s) 134, 262, 1310, 2493, 3125, 3257, 3873, 4388
BsaWI WCCGGW 3 cut(s) 600, 2400, 3454
Bsc4I CCNNNNNNNGG 6 cut(s) 256, 607, 1310, 3036, 3660, 4198
Bse118I RCCGGY 4 cut(s) 600, 689, 2161, 3148
Bse21I CCTNAGG 1 cut(s) 1950
Bse3DI GCAATG 3 cut(s) 775, 2339, 3017
Bse8I GATNNNNATC 1 cut(s) 2634
BseAI TCCGGA 1 cut(s) 3454
BseBI CCWGG 5 cut(s) 1311, 2907, 3641, 3661, 3826
BseCI ATCGAT 1 cut(s) 2221
BseDI CCNNGG 8 cut(s) 134, 262, 1310, 2493, 3125, 3257, 3873, 4388
BseJI GATNNNNATC 1 cut(s) 2634
BseLI CCNNNNNNNGG 6 cut(s) 256, 607, 1310, 3036, 3660, 4198
BseMI GCAATG 3 cut(s) 775, 2339, 3017
BseMII CTCAG 8 cut(s) 59, 123, 497, 608, 1941, 2583, 3474, 4158
BseRI GAGGAG 4 cut(s) 215, 218, 443, 1576
BseSI GKGCMC 2 cut(s) 4011, 4055
BseXI GCAGC 7 cut(s) 809, 1498, 2904, 3087, 3808, 4193, 4461
BseYI CCCAGC 2 cut(s) 970, 3770
BsgI GTGCAG 3 cut(s) 1165, 1523, 3378
Bsh1236I CGCG 3 cut(s) 763, 3117, 3171
Bsh1285I CGRYCG 2 cut(s) 2221, 3121
BshNI GGYRCC 1 cut(s) 3973
BshTI ACCGGT 1 cut(s) 600
BshVI ATCGAT 1 cut(s) 2221
BsiEI CGRYCG 2 cut(s) 2221, 3121
BsiHKAI GWGCWC 2 cut(s) 2383, 4011
BsiHKCI CYCGRG 2 cut(s) 3208, 4642
BsiSI CCGG 9 cut(s) 297, 330, 601, 690, 2162, 2401, 2685, 3149, 3455
BslFI GGGAC 5 cut(s) 1264, 2701, 3149, 3682, 4596
BslI CCNNNNNNNGG 6 cut(s) 256, 607, 1310, 3036, 3660, 4198
BsmAI GTCTC 7 cut(s) 1944, 2434, 2934, 3057, 3594, 3639, 3797
BsmFI GGGAC 5 cut(s) 1264, 2701, 3149, 3682, 4596
BsmI GAATGC 1 cut(s) 1788
Bso31I GGTCTC 1 cut(s) 3639
BsoBI CYCGRG 2 cut(s) 3208, 4642
Bsp119I TTCGAA 2 cut(s) 638, 2374
Bsp1286I GDGCHC 4 cut(s) 2128, 2383, 4011, 4055
Bsp13I TCCGGA 1 cut(s) 3454
Bsp1407I TGTACA 1 cut(s) 1319
Bsp19I CCATGG 1 cut(s) 2493
BspCNI CTCAG 8 cut(s) 58, 122, 498, 609, 1942, 2584, 3475, 4159
BspDI ATCGAT 1 cut(s) 2221
BspEI TCCGGA 1 cut(s) 3454
BspFNI CGCG 3 cut(s) 763, 3117, 3171
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 9 cut(s) 268, 1280, 2127, 2363, 2394, 3284, 3370, 3756, 3975
BspMI ACCTGC 3 cut(s) 1000, 1376, 2218
BspT104I TTCGAA 2 cut(s) 638, 2374
BspT107I GGYRCC 1 cut(s) 3973
BspTI CTTAAG 1 cut(s) 3866
BspTNI GGTCTC 1 cut(s) 3639
BsrDI GCAATG 3 cut(s) 775, 2339, 3017
BsrFI RCCGGY 4 cut(s) 600, 689, 2161, 3148
BsrGI TGTACA 1 cut(s) 1319
BssAI RCCGGY 4 cut(s) 600, 689, 2161, 3148
BssECI CCNNGG 8 cut(s) 134, 262, 1310, 2493, 3125, 3257, 3873, 4388
BssT1I CCWWGG 4 cut(s) 134, 2493, 3125, 3257
Bst2UI CCWGG 5 cut(s) 1311, 2907, 3641, 3661, 3826
Bst6I CTCTTC 4 cut(s) 166, 3393, 3963, 4587
BstAFI CTTAAG 1 cut(s) 3866
BstAUI TGTACA 1 cut(s) 1319
BstBAI YACGTR 1 cut(s) 3440
BstBI TTCGAA 2 cut(s) 638, 2374
BstDSI CCRYGG 3 cut(s) 262, 2493, 3873
BstENI CCTNNNNNAGG 1 cut(s) 1308
BstFNI CGCG 3 cut(s) 763, 3117, 3171
BstH2I RGCGCY 1 cut(s) 687
BstHHI GCGC 6 cut(s) 686, 918, 2528, 3075, 3117, 3173
BstMAI GTCTC 7 cut(s) 1944, 2434, 2934, 3057, 3594, 3639, 3797
BstMCI CGRYCG 2 cut(s) 2221, 3121
BstNI CCWGG 5 cut(s) 1311, 2907, 3641, 3661, 3826
BstNSI RCATGY 3 cut(s) 1478, 3810, 3821
BstSCI CCNGG 6 cut(s) 1309, 2684, 2905, 3639, 3659, 3824
BstSFI CTRYAG 2 cut(s) 2166, 3576
BstSLI GKGCMC 2 cut(s) 4011, 4055
BstUI CGCG 3 cut(s) 763, 3117, 3171
BstV1I GCAGC 7 cut(s) 809, 1498, 2904, 3087, 3808, 4193, 4461
BstV2I GAAGAC 3 cut(s) 522, 3011, 3933
BstX2I RGATCY 3 cut(s) 1354, 2969, 3656
BstXI CCANNNNNNTGG 4 cut(s) 2099, 2876, 3264, 4337
BstYI RGATCY 3 cut(s) 1354, 2969, 3656
Bsu15I ATCGAT 1 cut(s) 2221
Bsu36I CCTNAGG 1 cut(s) 1950
BsuI GTATCC 1 cut(s) 4512
BsuTUI ATCGAT 1 cut(s) 2221
BtgI CCRYGG 3 cut(s) 262, 2493, 3873
BtsIMutI CAGTG 9 cut(s) 526, 789, 1398, 1859, 1950, 2469, 3262, 4021, 4532
BveI ACCTGC 3 cut(s) 1000, 1376, 2218
CaiI CAGNNNCTG 3 cut(s) 1827, 1904, 1949
CciI TCATGA 1 cut(s) 814
CfoI GCGC 6 cut(s) 686, 918, 2528, 3075, 3117, 3173
Cfr10I RCCGGY 4 cut(s) 600, 689, 2161, 3148
ClaI ATCGAT 1 cut(s) 2221
CseI GACGC 3 cut(s) 769, 1417, 4512
CspAI ACCGGT 1 cut(s) 600
CspCI CAANNNNNGTGG 2 cut(s) 918, 953
DraI TTTAAA 1 cut(s) 1860
EaeI YGGCCR 5 cut(s) 699, 2159, 3038, 3137, 3344
Eam1104I CTCTTC 4 cut(s) 166, 3393, 3963, 4587
EarI CTCTTC 4 cut(s) 166, 3393, 3963, 4587
EciI GGCGGA 2 cut(s) 209, 4235
Eco130I CCWWGG 4 cut(s) 134, 2493, 3125, 3257
Eco24I GRGCYC 1 cut(s) 2128
Eco31I GGTCTC 1 cut(s) 3639
Eco32I GATATC 3 cut(s) 1039, 1053, 2589
Eco47I GGWCC 7 cut(s) 138, 327, 1278, 1757, 2089, 2403, 3718
Eco57I CTGAAG 7 cut(s) 765, 845, 1016, 1926, 3354, 3458, 3797
Eco72I CACGTG 1 cut(s) 3440
Eco81I CCTNAGG 1 cut(s) 1950
Eco88I CYCGRG 2 cut(s) 3208, 4642
EcoNI CCTNNNNNAGG 1 cut(s) 1308
EcoO109I RGGNCCY 3 cut(s) 138, 1278, 3368
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 5 cut(s) 1309, 2905, 3639, 3659, 3824
EcoRV GATATC 3 cut(s) 1039, 1053, 2589
EcoT14I CCWWGG 4 cut(s) 134, 2493, 3125, 3257
EcoT22I ATGCAT 1 cut(s) 4603
EcoT38I GRGCYC 1 cut(s) 2128
ErhI CCWWGG 4 cut(s) 134, 2493, 3125, 3257
FaqI GGGAC 5 cut(s) 1264, 2701, 3149, 3682, 4596
FauI CCCGC 2 cut(s) 44, 326
FauNDI CATATG 1 cut(s) 2554
FbaI TGATCA 3 cut(s) 1654, 2677, 3442
FblI GTMKAC 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 2128
FspI TGCGCA 2 cut(s) 917, 2527
GlaI GCGC 6 cut(s) 685, 917, 2527, 3074, 3116, 3172
GsaI CCCAGC 2 cut(s) 974, 3774
GsuI CTGGAG 4 cut(s) 1926, 3623, 3997, 4280
HaeII RGCGCY 1 cut(s) 687
HapII CCGG 9 cut(s) 297, 330, 601, 690, 2162, 2401, 2685, 3149, 3455
HgaI GACGC 3 cut(s) 769, 1417, 4512
HhaI GCGC 6 cut(s) 686, 918, 2528, 3075, 3117, 3173
Hin6I GCGC 6 cut(s) 684, 916, 2526, 3073, 3115, 3171
HinP1I GCGC 6 cut(s) 684, 916, 2526, 3073, 3115, 3171
HincII GTYRAC 2 cut(s) 390, 2194
HindII GTYRAC 2 cut(s) 390, 2194
HindIII AAGCTT 4 cut(s) 585, 1605, 3309, 4548
HpaI GTTAAC 1 cut(s) 2194
HpaII CCGG 9 cut(s) 297, 330, 601, 690, 2162, 2401, 2685, 3149, 3455
Hpy166II GTNNAC 9 cut(s) 390, 733, 2065, 2194, 2445, 3317, 3718, 3992, 4009
Hpy8I GTNNAC 9 cut(s) 390, 733, 2065, 2194, 2445, 3317, 3718, 3992, 4009
Hpy99I CGWCG 5 cut(s) 206, 409, 763, 1411, 3148
HpyCH4IV ACGT 3 cut(s) 2982, 3439, 3951
HpySE526I ACGT 3 cut(s) 2982, 3439, 3951
HspAI GCGC 6 cut(s) 684, 916, 2526, 3073, 3115, 3171
Kpn2I TCCGGA 1 cut(s) 3454
KroI GCCGGC 2 cut(s) 689, 2161
KroNI GCCGGC 2 cut(s) 691, 2163
Ksp22I TGATCA 3 cut(s) 1654, 2677, 3442
KspAI GTTAAC 1 cut(s) 2194
LmnI GCTCC 9 cut(s) 243, 252, 272, 765, 880, 2131, 2398, 3178, 3293
Lsp1109I GCAGC 7 cut(s) 809, 1498, 2904, 3087, 3808, 4193, 4461
MaeII ACGT 3 cut(s) 2982, 3439, 3951
MaeIII GTNAC 7 cut(s) 775, 1712, 2573, 2983, 3203, 3486, 3625
MfeI CAATTG 3 cut(s) 2304, 3607, 4507
MflI RGATCY 3 cut(s) 1354, 2969, 3656
MhlI GDGCHC 4 cut(s) 2128, 2383, 4011, 4055
MlsI TGGCCA 1 cut(s) 3346
MluNI TGGCCA 1 cut(s) 3346
MlyI GAGTC 8 cut(s) 56, 365, 374, 385, 395, 1406, 2326, 3200
MmeI TCCRAC 9 cut(s) 348, 513, 1474, 2567, 3254, 3524, 3728, 4480, 4511
Mox20I TGGCCA 1 cut(s) 3346
Mph1103I ATGCAT 1 cut(s) 4603
MreI CGCCGGCG 1 cut(s) 689
MroI TCCGGA 1 cut(s) 3454
MroNI GCCGGC 2 cut(s) 689, 2161
MroXI GAANNNNTTC 3 cut(s) 1091, 2076, 4211
MscI TGGCCA 1 cut(s) 3346
MslI CAYNNNNRTG 6 cut(s) 875, 1046, 2232, 3456, 4014, 4470
Msp20I TGGCCA 1 cut(s) 3346
MspA1I CMGCKG 2 cut(s) 1511, 4150
MspCI CTTAAG 1 cut(s) 3866
MspI CCGG 9 cut(s) 297, 330, 601, 690, 2162, 2401, 2685, 3149, 3455
MspR9I CCNGG 6 cut(s) 1311, 2686, 2907, 3641, 3661, 3826
MunI CAATTG 3 cut(s) 2304, 3607, 4507
Mva1269I GAATGC 1 cut(s) 1788
MvaI CCWGG 5 cut(s) 1311, 2907, 3641, 3661, 3826
MvnI CGCG 3 cut(s) 763, 3117, 3171
NaeI GCCGGC 2 cut(s) 691, 2163
NciI CCSGG 1 cut(s) 2686
NcoI CCATGG 1 cut(s) 2493
NdeI CATATG 1 cut(s) 2554
NgoMIV GCCGGC 2 cut(s) 689, 2161
NlaIV GGNNCC 9 cut(s) 268, 1280, 2127, 2363, 2394, 3284, 3370, 3756, 3975
NmeAIII GCCGAG 3 cut(s) 379, 2279, 4369
NmuCI GTSAC 3 cut(s) 775, 2573, 3203
NsbI TGCGCA 2 cut(s) 917, 2527
NsiI ATGCAT 1 cut(s) 4603
NspI RCATGY 3 cut(s) 1478, 3810, 3821
NspV TTCGAA 2 cut(s) 638, 2374
OliI CACNNNNGTG 1 cut(s) 4014
PaeI GCATGC 1 cut(s) 3821
PaeR7I CTCGAG 1 cut(s) 3208
PagI TCATGA 1 cut(s) 814
PaqCI CACCTGC 1 cut(s) 2218
PcsI WCGNNNNNNNCGW 3 cut(s) 2380, 3175, 4396
PctI GAATGC 1 cut(s) 1788
PdiI GCCGGC 2 cut(s) 691, 2163
PdmI GAANNNNTTC 3 cut(s) 1091, 2076, 4211
PfoI TCCNGGA 1 cut(s) 3659
PinAI ACCGGT 1 cut(s) 600
Ple19I CGATCG 2 cut(s) 2221, 3121
PleI GAGTC 8 cut(s) 56, 364, 373, 385, 394, 1405, 2326, 3200
PmaCI CACGTG 1 cut(s) 3440
PmlI CACGTG 1 cut(s) 3440
PpsI GAGTC 8 cut(s) 56, 364, 373, 385, 394, 1405, 2326, 3200
Ppu21I YACGTR 1 cut(s) 3440
PpuMI RGGWCCY 2 cut(s) 138, 1278
PsiI TTATAA 3 cut(s) 1374, 2634, 3899
Psp5II RGGWCCY 2 cut(s) 138, 1278
Psp6I CCWGG 5 cut(s) 1309, 2905, 3639, 3659, 3824
PspCI CACGTG 1 cut(s) 3440
PspFI CCCAGC 2 cut(s) 970, 3770
PspGI CCWGG 5 cut(s) 1309, 2905, 3639, 3659, 3824
PspN4I GGNNCC 9 cut(s) 268, 1280, 2127, 2363, 2394, 3284, 3370, 3756, 3975
PspPPI RGGWCCY 2 cut(s) 138, 1278
PspXI VCTCGAGB 1 cut(s) 3208
PstNI CAGNNNCTG 3 cut(s) 1827, 1904, 1949
PsuI RGATCY 3 cut(s) 1354, 2969, 3656
PvuI CGATCG 2 cut(s) 2221, 3121
PvuII CAGCTG 2 cut(s) 1511, 4150
RseI CAYNNNNRTG 6 cut(s) 875, 1046, 2232, 3456, 4014, 4470
SalI GTCGAC 1 cut(s) 388
ScaI AGTACT 1 cut(s) 3985
SchI GAGTC 8 cut(s) 56, 365, 374, 385, 395, 1406, 2326, 3200
ScrFI CCNGG 6 cut(s) 1311, 2686, 2907, 3641, 3661, 3826
SduI GDGCHC 4 cut(s) 2128, 2383, 4011, 4055
SfcI CTRYAG 2 cut(s) 2166, 3576
Sfr274I CTCGAG 1 cut(s) 3208
SfuI TTCGAA 2 cut(s) 638, 2374
SgrAI CRCCGGYG 2 cut(s) 689, 3148
SinI GGWCC 7 cut(s) 138, 327, 1278, 1757, 2089, 2403, 3718
SlaI CTCGAG 1 cut(s) 3208
SmiMI CAYNNNNRTG 6 cut(s) 875, 1046, 2232, 3456, 4014, 4470
SmlI CTYRAG 4 cut(s) 1558, 3208, 3866, 4255
SmoI CTYRAG 4 cut(s) 1558, 3208, 3866, 4255
SpeI ACTAGT 2 cut(s) 2560, 3707
SphI GCATGC 1 cut(s) 3821
SspI AATATT 1 cut(s) 3361
StyD4I CCNGG 6 cut(s) 1309, 2684, 2905, 3639, 3659, 3824
StyI CCWWGG 4 cut(s) 134, 2493, 3125, 3257
TaiI ACGT 3 cut(s) 2985, 3442, 3954
TatI WGTACW 6 cut(s) 1319, 1361, 2444, 2661, 2801, 3983
TauI GCSGC 5 cut(s) 689, 701, 3040, 3139, 3481
TscAI CASTG 9 cut(s) 526, 789, 1398, 1859, 1950, 2476, 3269, 4021, 4532
TseFI GTSAC 3 cut(s) 775, 2573, 3203
TseI GCWGC 7 cut(s) 797, 1511, 2917, 3075, 3821, 4181, 4449
Tsp45I GTSAC 3 cut(s) 775, 2573, 3203
TspGWI ACGGA 3 cut(s) 2784, 3520, 4396
TspRI CASTG 9 cut(s) 526, 789, 1398, 1859, 1950, 2476, 3269, 4021, 4532
Vha464I CTTAAG 1 cut(s) 3866
VneI GTGCAC 1 cut(s) 4007
VpaK11BI GGWCC 7 cut(s) 138, 327, 1278, 1757, 2089, 2403, 3718
XagI CCTNNNNNAGG 1 cut(s) 1308
XceI RCATGY 3 cut(s) 1478, 3810, 3821
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XhoI CTCGAG 1 cut(s) 3208
XmaJI CCTAGG 2 cut(s) 134, 3125
XmiI GTMKAC 1 cut(s) 389
XmnI GAANNNNTTC 3 cut(s) 1091, 2076, 4211
ZrmI AGTACT 1 cut(s) 3985
Zsp2I ATGCAT 1 cut(s) 4603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.