RLG00000028609

Nucleotidyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003190
Physical Location & Seq
Forward (+)
25000602 .. 25013317
12716 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028609

Sequence Viewer

Length: 4905 bp
ATGTCGAAACCAAACCCTAAATCCCCTTACAATTCCCGCATAACCCAAAACCAACTCATCGACTCCCTCACCTCCCACATCTCCCTCTACAATTCCCACTCCCATTCCTCAGACCCCAAGCCCAACCCTAACCCTAGGTCCTCCATCCTCAAATGGTTCTCCTCCCTCACCCTCCACCACCGCCAAGCTCACCTCACCGCCGTTGATCCCAAATTCGTCCGCCTCCTCCTCCAAATGCTCCACAAGCTCCGCACGCACGGCCACGGCTCCTTCATCATCCTCCCCGACCTCCCCTCCGGCGACCTCCCCACGCTCTGTTTCAAGAGGTCCGGCGGGCTTTTGTCTAGGGTTGCCGAGTCGAATGAGTCGGAGAAGATGGTGTTCGATTCGACTCGGTTGTTTTCGTCGAGGGAGGGAGAGAAGGTCGAGGAGTGTTCGTGTTCGGTGAGGGATATTGATACGGTGACGGTGAGTGAGGAGTTGGTTGAGAATTTAGATAGGTTTGTGGAGGCTATGGATGATATATCAAATGGTGGCTTCCTGAGAGGTGAAGACAGTGATTTGGGGTCGGATTGGGTGGAATTGAAGTGGTTGAAGGACAAAGGGTATTACAGTATGGAAGCTTTCGTGGCGAACCGGTTAGAGGTTGCTCTGAGGCTTGCTTGGCTGAATTCGAATAATGCAAGGAAAAGAGGGGTGAAGTTGAAAGAGAAAATTAGTGTGGCGGCTGTGGCGGCCAATGTGTTTTGGAGGAAGAAGGGGTGTGTGGACTGGTGGGGGAATTTGGATGATGTGACGCGGAGCAATGTAGTGACTTCAGTGTTGGGGAAAGCAGCAAAACCGTTGATTCATGAGATTCTGAAGGGAACAAGTAGTGGGGTAGAGGATGAAATGTGGCTCGCCAACACAGGAATGGAGCAACCATTGAGGTACAACCATAATGTATCTATGCGCAAGACTGTTCCAAAACTTGTGGCTGACACAGAATTTGGGTCAAGCATTATCCCAGCTTCTCTTTCTGGGAAACCTGCTTCCTTAGCCGATGCTTTTAATAATCTCTTTGTGCTTCAGGATATCATAACGATGATATCATTATGTCGCCATAATGAATATGATAAAGGGAAATTCTTCTATAGCACATTGAGTTCAATTTCTACCATTTCTGATTTTATACTAAGAAAAATACGAGGATTTCTCATGGTTCTTTTGCTTGACTGCACAAAACTTGAACTTTTAGCAGAGGGAAATGAAAAATGCTTACCTAAGAAACCCAAAACAAAGCCTAGTGCTTCTAGCCGTAAAAGTAAGGGGAGGTCCCACAATATGAAAAGGCCAAATCCTGTTCCAAGGTCATGTACAGATGAATTTTTGTGTGAAACATCTGCTAAGGATCTTAGTACATTGGCTTATAAAGAGAAGGCAGATTCAGTGGAGTCCAAGACGACGCATACTACACATCAAGAGAAGGAGATTTCCAGAGAATCGTCATCATCAAAAGATGAAATGGAACATGCACAAGCATTGGCTGTTGGAAAAGTGCAGACAGCTGCGAGGAAGGGTAGGAAAGGAAAAGGCAAAAAGAAAATAAATGGCTTGAGGAGTGCTAATGATATGGAGAAATTTGATAGATCATTCATGGAAGCTTCTTCTTCTTCTTCAGTCATTCTTGAAGATGACGCAGCAAAGTCTGATCAGGTTTCTGGTGATACAGCCTTCCAGAACATCAGATGTGATAATTCAGCTAGTTGTAACAGCCCTTTGTCAAATTCAATTCCTTCTGGTTCTGCGAATGGACCTCTTAAAGATGAAGATGCCACCAAAAGCATTCAAGAGAATGATGGTATTGGTTCTGGTGCCAGTTTCTGTCATAAGGTTCTGGAAAGTTACCAGTGTTTAAATAATAATACTGAAATCCAAACAAAATGTTCTGGATCAGAAACTGAAGCCTGTAAGGTAGATGGACACTTGATAAAGTCTCCAGTGCCTGAGGTAGATGATAAAGTTTCCCATCATAAAGATATTGACTTGCAGGACAAGCGTGCTGTTAAGTCAGGTGCGAAAGAAGTTTTGCCAGCCAAAGAAATAAGAGCTTCTGATGTAAACGAGGAAGCTGTTCTGTTGCAGGACCAAGAGAGTGGAAATAATATATATCACACTAGGGCTCCAAGTTCTTTTGATTGCCCTCCTTATGAGTGGCCGGGTGTAGCTTGTGCCTATTTTCCACCTGTTAACTCACATCTCCCACCTGCCACCGATCGATTGCATCTGGATGTTGGTCATAACTGGCAGAATCACCGCCAGTCTTTTCTACCCACAATACATCAGGTGAGAAACTCGGCAATTGAAGGTGGGTGTAATCCAGTTCTGACTCGACCATTGCCAATGAGTATAGATTGGCCCCCAATGGTTCGAAGTGCTCGTAGAGTGGCTCCATACCGGACCTGTAATTATGATTCTGGGTTTGTCTCAAAAAAGCAGTGTACTTATCCACAGGGTTTTGCACACACTGTCCAAATAAATGCAACAACCATGGATGATGAAAGGAAGTATTCTTGGGATTGCGCAGACCTACCTGATCCAACAAAGGCATATGAACTAGTGGATGAATGTGACAGTCAATGGATATCTGAGGATGAAGTTGAGGTGCAAGCATTATCTGGGGCAGATTATAATCAATACTTTGGTGGTGGTGTGATGTACTGGAATCCTTCTGATCATACCGGGACAGTTTTCTCTCGCCCTCCTTCCCTTAGTTCTGATGATAGCTCATGGGCTTGGCGTGAAGCTGACATAAATAGAGCTGTTGATGATATGGTTGCATTTTCTTCTCCGTACAGTACAAATGGTTTGACTTCACCTACTGCTTCCTTCTGTTCTCCGTTTGATCCTTTGGGATCAGGAAACCAGCCTCTTGGATATGTTATGTCAGGAAATGAAGTACCAGGCAAGGTGCTGCATCCCTCATCAGCAATGGGAGACACAGTAGTAGATGAGGAATCGTCTGGATCTTTGGCTGACATAACTGGTGATATTGAAGGGAAGACAGGCGATTCATTGCCTTACCCCATGTTGCGGCCAATCATCATATCAAGATCAAGAGACTTTAAGCGCAGCCATGATCGTAAAAGCCCATGTGTTCCTCCTACTATGCGCGATCGACCTAGGATAAAGCGGCCACCGTCGCCGGTGGTGCTTTCTGTCCCACGCGCTCCACGACCACCTCCACCATCTTCTGTGAGTGACTCGAGGAAACACAGGGGATTTCCAACTGTTCGTTCTGGTAGCTCGAGCCCAAGGCACTGGGGTATGAGAGGTTGGTTCCATGATGGAGCTAACTTGGAGGAAGCTTGTTTACGCATGGATGGTGCTGAAGTTGTTTGGCCATTTAGAAATAATATTTCAGGCCCCCCATTGATTCAGCCTCTTCCTGCACCTCTGTTGCAGGATCGACTGATTGCTATTTCTCAACTAGCACGTGATCAGGAACATCCGGATGTTGCATTTCCCCTTCAGCCGCCTGAGTTACATAACTGTCCAACACGGAAGGCATCTCTCTCTCTGATGCACAGCCTTGTGCATGATGAGATTGATTTTTTTTGCAAGAAGGTTGCTACGGAGAATATGTCTCGGAAGCCCTACATCAATTGGGCTGTCAAACGAGTTACACGGTCTCTCCAGGTGCTTTGGCCCAGATCCAGGACAAATATATTTGGTTCAGTTGCAAACGGTTTGTCCCTTCCAACTAGTGATGTGGACCTTGTGGTTTCTCTACCTCCTGTTAGGAATTTGGAACCCATTAAAGAAGCTGGGATATTGGAGGGTCGAAATGGTATTAAAGAGACATGCCTTCAGCATGCTGCCAGGTATCTCGTCAATCAGGATTGGGTTAAAAATGATTCTCTTAAGACCGTGGAAAATACAGCTATACCGATTATAATGCTTGTGGTGGAAGTTCCCAATGATCTAATTGCCTCGTCTTCGTCTAACGTGCAATCACCAAAAGAAGAGGCACCTCACAGTACTGGTGAACCAGACTGTAATGTGCACTCCAGTGGGGTTATTTTAGAAGAATCTGCTATGCCTAAGTGCCCCCAGATAACTTGTGATGCTACAAAGGATTCAGTATCAATTCGCATTGACATCAGTTTCAAATCTCCATCTCATACAGGATTTCAAACTACACAGTTGGTTAAAGATTTAACTGAGCAGTTTCCAGCTGCTACACCCCTTGCTTTGGTACTGAAACAGTTCTTGGCGGATCGTAGTCTGGATCAGTCTTATTCTGGTGGCTTGAGTTCCTACTGCTTGGTACTACTAATTGTGCGTTTTCTCCAGCATGAGTATCATCTTAGCCGACCTATCAACCAAAACTTTGGAAACCTTCTGATGAATTTTCTTTATTTTTTTGGGAATGTTTTTGATCCTCGGCAAATGCGTATTTCCGTACAGGGAAGTGGTGTATATATAAAGAGGGAAAGAGGTTGCAGCATCGACCCCATACACATTGATGATCCCCTTTATCCGACTAATAATGTCGGACGCAATTGCTTTCGGATACATCAGTGTATCAAGGCGTTTTCAGAAGCTTATTCTATTTTGGAGAATGAGCTTGCTTCTCTTCCTAATGATGATGATGCATCTTCAAGTCATATGTGGCAGAAGGTGGGCGGGAAGGAAAGTGGCATTATAGTAGTTGAAATGGGGAGTTTGCCAGCTGCAGTCTGTGTACATCTTCGAGTCTGGTTTGACTTGTCAAATACTGTGCCACCTCCCTTCATTAGTAGGACCAGGAGATTGAGGAGCCATAATCATCACCGCGTACATTGCCTCTCAAAGTCTTGGTACACTTATGAACTCTGCTCATATGGTTCTGCTACAGGAGTCGGCTGCAGAAAGCAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

1635

Amino Acids

181.26

Weight (kDa)

6.91

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 1217 - 1313 2e-07 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1410, 2670, 3935
AarI CACCTGC 1 cut(s) 2254
Acc16I TGCGCA 2 cut(s) 953, 2563
Acc36I ACCTGC 2 cut(s) 1036, 2254
AccB1I GGYRCC 2 cut(s) 1853, 4009
AccII CGCG 4 cut(s) 799, 3153, 3207, 4818
AccIII TCCGGA 1 cut(s) 3490
AcoI YGGCCR 6 cut(s) 259, 735, 2195, 3074, 3173, 3380
AcuI CTGAAG 8 cut(s) 801, 881, 1052, 1641, 1962, 3390, 3494, 3833
AcvI CACGTG 1 cut(s) 3476
AfiI CCNNNNNNNGG 4 cut(s) 643, 3072, 3696, 4234
AflII CTTAAG 1 cut(s) 3902
AgeI ACCGGT 1 cut(s) 636
AhlI ACTAGT 2 cut(s) 2596, 3743
AjnI CCWGG 5 cut(s) 2941, 3675, 3695, 3860, 4787
AjuI GAANNNNNNNTTGG 4 cut(s) 3259, 3291, 4235, 4267
AleI CACNNNNGTG 1 cut(s) 4050
Alw21I GWGCWC 2 cut(s) 2419, 4047
Alw26I GTCTC 7 cut(s) 1980, 2470, 2970, 3093, 3630, 3675, 3833
Alw44I GTGCAC 1 cut(s) 4043
AlwNI CAGNNNCTG 3 cut(s) 1863, 1940, 1985
Ama87I CYCGRG 2 cut(s) 3244, 3286
Aor13HI TCCGGA 1 cut(s) 3490
ApaLI GTGCAC 1 cut(s) 4043
ArsI GACNNNNNNTTYG 4 cut(s) 971, 1003, 3716, 3748
AsiGI ACCGGT 1 cut(s) 636
Asp700I GAANNNNTTC 3 cut(s) 1127, 2112, 4247
AspA2I CCTAGG 2 cut(s) 134, 3161
AspLEI GCGC 5 cut(s) 954, 2564, 3111, 3153, 3209
AsuC2I CCSGG 2 cut(s) 2199, 2722
AsuII TTCGAA 2 cut(s) 674, 2410
AvaI CYCGRG 2 cut(s) 3244, 3286
AvaII GGWCC 8 cut(s) 138, 327, 1314, 1793, 2125, 2439, 3754, 4785
AvrII CCTAGG 2 cut(s) 134, 3161
AxyI CCTNAGG 1 cut(s) 1986
BaeGI GKGCMC 2 cut(s) 4047, 4091
BaeI ACNNNNGTAYC 2 cut(s) 4547, 4580
BalI TGGCCA 1 cut(s) 3382
BanI GGYRCC 2 cut(s) 1853, 4009
BanII GRGCYC 2 cut(s) 2164, 3293
BarI GAAGNNNNNNTAC 2 cut(s) 3336, 3368
BbrPI CACGTG 1 cut(s) 3476
BbsI GAAGAC 3 cut(s) 558, 3047, 3969
Bbv12I GWGCWC 2 cut(s) 2419, 4047
BccI CCATC 9 cut(s) 152, 370, 1832, 1952, 2016, 3235, 3320, 3356, 4165
BceAI ACGGC 4 cut(s) 185, 274, 280, 1281
BcgI CGANNNNNNTGC 2 cut(s) 3919, 3953
BciT130I CCWGG 5 cut(s) 2943, 3677, 3697, 3862, 4789
BciVI GTATCC 1 cut(s) 4548
BclI TGATCA 3 cut(s) 1690, 2713, 3478
BcnI CCSGG 2 cut(s) 2199, 2722
BcoDI GTCTC 7 cut(s) 1980, 2470, 2970, 3093, 3630, 3675, 3833
BcuI ACTAGT 2 cut(s) 2596, 3743
BfmI CTRYAG 4 cut(s) 1132, 4716, 4875, 4888
BfrI CTTAAG 1 cut(s) 3902
BfuAI ACCTGC 2 cut(s) 1036, 2254
BfuI GTATCC 1 cut(s) 4548
BlnI CCTAGG 2 cut(s) 134, 3161
BmcAI AGTACT 1 cut(s) 4021
Bme1390I CCNGG 7 cut(s) 2199, 2722, 2943, 3677, 3697, 3862, 4789
Bme18I GGWCC 8 cut(s) 138, 327, 1314, 1793, 2125, 2439, 3754, 4785
BmeT110I CYCGRG 2 cut(s) 3244, 3286
BmrFI CCNGG 7 cut(s) 2199, 2722, 2943, 3677, 3697, 3862, 4789
BmrI ACTGGG 1 cut(s) 3310
BmuI ACTGGG 1 cut(s) 3310
BpiI GAAGAC 3 cut(s) 558, 3047, 3969
BplI GAGNNNNNCTC 2 cut(s) 1179, 1211
BpmI CTGGAG 4 cut(s) 1962, 3659, 4033, 4316
Bpu10I CCTNAGC 2 cut(s) 1036, 1386
Bpu14I TTCGAA 2 cut(s) 674, 2410
BpuEI CTTGAG 2 cut(s) 1615, 4312
BpuMI CCSGG 2 cut(s) 2199, 2722
Bsa29I ATCGAT 1 cut(s) 2257
BsaAI YACGTR 1 cut(s) 3476
BsaBI GATNNNNATC 1 cut(s) 2670
BsaI GGTCTC 1 cut(s) 3675
BsaJI CCNNGG 8 cut(s) 134, 262, 1346, 2529, 3161, 3293, 3909, 4424
BsaWI WCCGGW 3 cut(s) 636, 2436, 3490
Bsc4I CCNNNNNNNGG 4 cut(s) 643, 3072, 3696, 4234
Bse118I RCCGGY 2 cut(s) 636, 3184
Bse21I CCTNAGG 1 cut(s) 1986
Bse3DI GCAATG 5 cut(s) 811, 2375, 2976, 3053, 4822
Bse8I GATNNNNATC 1 cut(s) 2670
BseAI TCCGGA 1 cut(s) 3490
BseBI CCWGG 5 cut(s) 2943, 3677, 3697, 3862, 4789
BseCI ATCGAT 1 cut(s) 2257
BseDI CCNNGG 8 cut(s) 134, 262, 1346, 2529, 3161, 3293, 3909, 4424
BseJI GATNNNNATC 1 cut(s) 2670
BseLI CCNNNNNNNGG 4 cut(s) 643, 3072, 3696, 4234
BseMI GCAATG 5 cut(s) 811, 2375, 2976, 3053, 4822
BseMII CTCAG 7 cut(s) 123, 533, 644, 1977, 2619, 3510, 4194
BseRI GAGGAG 7 cut(s) 151, 215, 218, 443, 491, 1612, 4813
BseSI GKGCMC 2 cut(s) 4047, 4091
BseYI CCCAGC 2 cut(s) 1006, 3806
BsgI GTGCAG 3 cut(s) 1201, 1559, 3414
Bsh1236I CGCG 4 cut(s) 799, 3153, 3207, 4818
Bsh1285I CGRYCG 2 cut(s) 2257, 3157
BshNI GGYRCC 2 cut(s) 1853, 4009
BshTI ACCGGT 1 cut(s) 636
BshVI ATCGAT 1 cut(s) 2257
BsiEI CGRYCG 2 cut(s) 2257, 3157
BsiHKAI GWGCWC 2 cut(s) 2419, 4047
BsiHKCI CYCGRG 2 cut(s) 3244, 3286
BsiSI CCGG 8 cut(s) 297, 330, 637, 2198, 2437, 2721, 3185, 3491
BslFI GGGAC 4 cut(s) 1300, 2737, 3185, 3718
BslI CCNNNNNNNGG 4 cut(s) 643, 3072, 3696, 4234
BsmAI GTCTC 7 cut(s) 1980, 2470, 2970, 3093, 3630, 3675, 3833
BsmFI GGGAC 4 cut(s) 1300, 2737, 3185, 3718
BsmI GAATGC 1 cut(s) 1824
Bso31I GGTCTC 1 cut(s) 3675
BsoBI CYCGRG 2 cut(s) 3244, 3286
Bsp119I TTCGAA 2 cut(s) 674, 2410
Bsp1286I GDGCHC 5 cut(s) 2164, 2419, 3293, 4047, 4091
Bsp13I TCCGGA 1 cut(s) 3490
Bsp1407I TGTACA 2 cut(s) 1355, 4726
Bsp19I CCATGG 1 cut(s) 2529
BspCNI CTCAG 7 cut(s) 122, 534, 645, 1978, 2620, 3511, 4195
BspDI ATCGAT 1 cut(s) 2257
BspEI TCCGGA 1 cut(s) 3490
BspFNI CGCG 4 cut(s) 799, 3153, 3207, 4818
BspHI TCATGA 1 cut(s) 850
BspMAI CTGCAG 2 cut(s) 4720, 4892
BspMI ACCTGC 2 cut(s) 1036, 2254
BspT104I TTCGAA 2 cut(s) 674, 2410
BspT107I GGYRCC 2 cut(s) 1853, 4009
BspTI CTTAAG 1 cut(s) 3902
BspTNI GGTCTC 1 cut(s) 3675
BsrDI GCAATG 5 cut(s) 811, 2375, 2976, 3053, 4822
BsrFI RCCGGY 2 cut(s) 636, 3184
BsrGI TGTACA 2 cut(s) 1355, 4726
BssAI RCCGGY 2 cut(s) 636, 3184
BssECI CCNNGG 8 cut(s) 134, 262, 1346, 2529, 3161, 3293, 3909, 4424
BssT1I CCWWGG 5 cut(s) 134, 1346, 2529, 3161, 3293
Bst2UI CCWGG 5 cut(s) 2943, 3677, 3697, 3862, 4789
Bst6I CTCTTC 3 cut(s) 3429, 3999, 4623
BstAFI CTTAAG 1 cut(s) 3902
BstAUI TGTACA 2 cut(s) 1355, 4726
BstBAI YACGTR 1 cut(s) 3476
BstBI TTCGAA 2 cut(s) 674, 2410
BstDSI CCRYGG 3 cut(s) 262, 2529, 3909
BstFNI CGCG 4 cut(s) 799, 3153, 3207, 4818
BstHHI GCGC 5 cut(s) 954, 2564, 3111, 3153, 3209
BstMAI GTCTC 7 cut(s) 1980, 2470, 2970, 3093, 3630, 3675, 3833
BstMCI CGRYCG 2 cut(s) 2257, 3157
BstNI CCWGG 5 cut(s) 2943, 3677, 3697, 3862, 4789
BstNSI RCATGY 3 cut(s) 1514, 3846, 3857
BstSCI CCNGG 7 cut(s) 2197, 2720, 2941, 3675, 3695, 3860, 4787
BstSFI CTRYAG 4 cut(s) 1132, 4716, 4875, 4888
BstSLI GKGCMC 2 cut(s) 4047, 4091
BstUI CGCG 4 cut(s) 799, 3153, 3207, 4818
BstV2I GAAGAC 3 cut(s) 558, 3047, 3969
BstX2I RGATCY 3 cut(s) 1390, 3005, 3692
BstXI CCANNNNNNTGG 4 cut(s) 2135, 2912, 3300, 4373
BstYI RGATCY 3 cut(s) 1390, 3005, 3692
Bsu15I ATCGAT 1 cut(s) 2257
Bsu36I CCTNAGG 1 cut(s) 1986
BsuI GTATCC 1 cut(s) 4548
BsuTUI ATCGAT 1 cut(s) 2257
BtgI CCRYGG 3 cut(s) 262, 2529, 3909
BtsI GCAGTG 1 cut(s) 2483
BveI ACCTGC 2 cut(s) 1036, 2254
CaiI CAGNNNCTG 3 cut(s) 1863, 1940, 1985
CciI TCATGA 1 cut(s) 850
CfoI GCGC 5 cut(s) 954, 2564, 3111, 3153, 3209
Cfr10I RCCGGY 2 cut(s) 636, 3184
ClaI ATCGAT 1 cut(s) 2257
CseI GACGC 4 cut(s) 805, 1453, 1685, 4548
CspAI ACCGGT 1 cut(s) 636
CspCI CAANNNNNGTGG 2 cut(s) 954, 989
DraI TTTAAA 1 cut(s) 1896
EaeI YGGCCR 6 cut(s) 259, 735, 2195, 3074, 3173, 3380
Eam1104I CTCTTC 3 cut(s) 3429, 3999, 4623
EarI CTCTTC 3 cut(s) 3429, 3999, 4623
EciI GGCGGA 2 cut(s) 209, 4271
Eco130I CCWWGG 5 cut(s) 134, 1346, 2529, 3161, 3293
Eco24I GRGCYC 2 cut(s) 2164, 3293
Eco31I GGTCTC 1 cut(s) 3675
Eco32I GATATC 3 cut(s) 1075, 1089, 2625
Eco47I GGWCC 8 cut(s) 138, 327, 1314, 1793, 2125, 2439, 3754, 4785
Eco57I CTGAAG 8 cut(s) 801, 881, 1052, 1641, 1962, 3390, 3494, 3833
Eco72I CACGTG 1 cut(s) 3476
Eco81I CCTNAGG 1 cut(s) 1986
Eco88I CYCGRG 2 cut(s) 3244, 3286
EcoO109I RGGNCCY 3 cut(s) 138, 1314, 3404
EcoRI GAATTC 1 cut(s) 670
EcoRII CCWGG 5 cut(s) 2941, 3675, 3695, 3860, 4787
EcoRV GATATC 3 cut(s) 1075, 1089, 2625
EcoT14I CCWWGG 5 cut(s) 134, 1346, 2529, 3161, 3293
EcoT22I ATGCAT 1 cut(s) 4639
EcoT38I GRGCYC 2 cut(s) 2164, 3293
ErhI CCWWGG 5 cut(s) 134, 1346, 2529, 3161, 3293
FaqI GGGAC 4 cut(s) 1300, 2737, 3185, 3718
FauI CCCGC 3 cut(s) 44, 326, 4661
FauNDI CATATG 3 cut(s) 2590, 4650, 4864
FbaI TGATCA 3 cut(s) 1690, 2713, 3478
FriOI GRGCYC 2 cut(s) 2164, 3293
FspI TGCGCA 2 cut(s) 953, 2563
GlaI GCGC 5 cut(s) 953, 2563, 3110, 3152, 3208
GsaI CCCAGC 2 cut(s) 1010, 3810
GsuI CTGGAG 4 cut(s) 1962, 3659, 4033, 4316
HapII CCGG 8 cut(s) 297, 330, 637, 2198, 2437, 2721, 3185, 3491
HgaI GACGC 4 cut(s) 805, 1453, 1685, 4548
HhaI GCGC 5 cut(s) 954, 2564, 3111, 3153, 3209
Hin6I GCGC 5 cut(s) 952, 2562, 3109, 3151, 3207
HinP1I GCGC 5 cut(s) 952, 2562, 3109, 3151, 3207
HincII GTYRAC 1 cut(s) 2230
HindII GTYRAC 1 cut(s) 2230
HindIII AAGCTT 4 cut(s) 621, 1641, 3345, 4584
HpaI GTTAAC 1 cut(s) 2230
HpaII CCGG 8 cut(s) 297, 330, 637, 2198, 2437, 2721, 3185, 3491
Hpy99I CGWCG 3 cut(s) 409, 1447, 3184
HpyCH4IV ACGT 2 cut(s) 3475, 3987
HpySE526I ACGT 2 cut(s) 3475, 3987
HspAI GCGC 5 cut(s) 952, 2562, 3109, 3151, 3207
Kpn2I TCCGGA 1 cut(s) 3490
Ksp22I TGATCA 3 cut(s) 1690, 2713, 3478
KspAI GTTAAC 1 cut(s) 2230
MaeII ACGT 2 cut(s) 3475, 3987
MaeIII GTNAC 9 cut(s) 463, 793, 811, 1748, 1883, 2609, 3239, 3522, 3661
MfeI CAATTG 3 cut(s) 2340, 3643, 4543
MflI RGATCY 3 cut(s) 1390, 3005, 3692
MhlI GDGCHC 5 cut(s) 2164, 2419, 3293, 4047, 4091
MlsI TGGCCA 1 cut(s) 3382
MluNI TGGCCA 1 cut(s) 3382
MlyI GAGTC 9 cut(s) 56, 365, 374, 385, 1442, 2362, 3236, 4746, 4890
MmeI TCCRAC 9 cut(s) 348, 549, 1510, 2603, 3290, 3560, 3764, 4516, 4547
Mox20I TGGCCA 1 cut(s) 3382
Mph1103I ATGCAT 1 cut(s) 4639
MroI TCCGGA 1 cut(s) 3490
MroXI GAANNNNTTC 3 cut(s) 1127, 2112, 4247
MscI TGGCCA 1 cut(s) 3382
MslI CAYNNNNRTG 6 cut(s) 911, 1082, 2268, 3492, 4050, 4506
Msp20I TGGCCA 1 cut(s) 3382
MspA1I CMGCKG 3 cut(s) 1547, 4217, 4715
MspCI CTTAAG 1 cut(s) 3902
MspI CCGG 8 cut(s) 297, 330, 637, 2198, 2437, 2721, 3185, 3491
MspR9I CCNGG 7 cut(s) 2199, 2722, 2943, 3677, 3697, 3862, 4789
MunI CAATTG 3 cut(s) 2340, 3643, 4543
Mva1269I GAATGC 1 cut(s) 1824
MvaI CCWGG 5 cut(s) 2943, 3677, 3697, 3862, 4789
MvnI CGCG 4 cut(s) 799, 3153, 3207, 4818
NciI CCSGG 2 cut(s) 2199, 2722
NcoI CCATGG 1 cut(s) 2529
NdeI CATATG 3 cut(s) 2590, 4650, 4864
NmeAIII GCCGAG 3 cut(s) 379, 2315, 4405
NmuCI GTSAC 5 cut(s) 463, 793, 811, 2609, 3239
NsbI TGCGCA 2 cut(s) 953, 2563
NsiI ATGCAT 1 cut(s) 4639
NspI RCATGY 3 cut(s) 1514, 3846, 3857
NspV TTCGAA 2 cut(s) 674, 2410
OliI CACNNNNGTG 1 cut(s) 4050
PaeI GCATGC 1 cut(s) 3857
PaeR7I CTCGAG 2 cut(s) 3244, 3286
PagI TCATGA 1 cut(s) 850
PaqCI CACCTGC 1 cut(s) 2254
PcsI WCGNNNNNNNCGW 3 cut(s) 2416, 3211, 4432
PctI GAATGC 1 cut(s) 1824
PdmI GAANNNNTTC 3 cut(s) 1127, 2112, 4247
PfoI TCCNGGA 1 cut(s) 3695
PinAI ACCGGT 1 cut(s) 636
Ple19I CGATCG 2 cut(s) 2257, 3157
PleI GAGTC 9 cut(s) 56, 364, 373, 385, 1441, 2362, 3236, 4745, 4889
PmaCI CACGTG 1 cut(s) 3476
PmlI CACGTG 1 cut(s) 3476
PpsI GAGTC 9 cut(s) 56, 364, 373, 385, 1441, 2362, 3236, 4745, 4889
Ppu21I YACGTR 1 cut(s) 3476
PpuMI RGGWCCY 2 cut(s) 138, 1314
PsiI TTATAA 3 cut(s) 1410, 2670, 3935
Psp5II RGGWCCY 2 cut(s) 138, 1314
Psp6I CCWGG 5 cut(s) 2941, 3675, 3695, 3860, 4787
PspCI CACGTG 1 cut(s) 3476
PspFI CCCAGC 2 cut(s) 1006, 3806
PspGI CCWGG 5 cut(s) 2941, 3675, 3695, 3860, 4787
PspPPI RGGWCCY 2 cut(s) 138, 1314
PspXI VCTCGAGB 2 cut(s) 3244, 3286
PstI CTGCAG 2 cut(s) 4720, 4892
PstNI CAGNNNCTG 3 cut(s) 1863, 1940, 1985
PsuI RGATCY 3 cut(s) 1390, 3005, 3692
PvuI CGATCG 2 cut(s) 2257, 3157
PvuII CAGCTG 3 cut(s) 1547, 4217, 4715
RseI CAYNNNNRTG 6 cut(s) 911, 1082, 2268, 3492, 4050, 4506
ScaI AGTACT 1 cut(s) 4021
SchI GAGTC 9 cut(s) 56, 365, 374, 385, 1442, 2362, 3236, 4746, 4890
ScrFI CCNGG 7 cut(s) 2199, 2722, 2943, 3677, 3697, 3862, 4789
SduI GDGCHC 5 cut(s) 2164, 2419, 3293, 4047, 4091
SfcI CTRYAG 4 cut(s) 1132, 4716, 4875, 4888
Sfr274I CTCGAG 2 cut(s) 3244, 3286
SfuI TTCGAA 2 cut(s) 674, 2410
SgrAI CRCCGGYG 1 cut(s) 3184
SinI GGWCC 8 cut(s) 138, 327, 1314, 1793, 2125, 2439, 3754, 4785
SlaI CTCGAG 2 cut(s) 3244, 3286
SmiMI CAYNNNNRTG 6 cut(s) 911, 1082, 2268, 3492, 4050, 4506
SmlI CTYRAG 5 cut(s) 1594, 3244, 3286, 3902, 4291
SmoI CTYRAG 5 cut(s) 1594, 3244, 3286, 3902, 4291
SpeI ACTAGT 2 cut(s) 2596, 3743
SphI GCATGC 1 cut(s) 3857
SspI AATATT 1 cut(s) 3397
StyD4I CCNGG 7 cut(s) 2197, 2720, 2941, 3675, 3695, 3860, 4787
StyI CCWWGG 5 cut(s) 134, 1346, 2529, 3161, 3293
TaiI ACGT 2 cut(s) 3478, 3990
TatI WGTACW 7 cut(s) 1355, 1397, 2480, 2697, 2837, 4019, 4726
TauI GCSGC 5 cut(s) 728, 737, 3076, 3175, 3517
TseFI GTSAC 5 cut(s) 463, 793, 811, 2609, 3239
Tsp45I GTSAC 5 cut(s) 463, 793, 811, 2609, 3239
TspGWI ACGGA 5 cut(s) 2820, 2868, 3556, 3629, 4432
Vha464I CTTAAG 1 cut(s) 3902
VneI GTGCAC 1 cut(s) 4043
VpaK11BI GGWCC 8 cut(s) 138, 327, 1314, 1793, 2125, 2439, 3754, 4785
XceI RCATGY 3 cut(s) 1514, 3846, 3857
XcmI CCANNNNNNNNNTGG 1 cut(s) 910
XhoI CTCGAG 2 cut(s) 3244, 3286
XmaJI CCTAGG 2 cut(s) 134, 3161
XmnI GAANNNNTTC 3 cut(s) 1127, 2112, 4247
ZrmI AGTACT 1 cut(s) 4021
Zsp2I ATGCAT 1 cut(s) 4639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.