Rh1BG182800

Nucleotidyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003190
Physical Location & Seq
Reverse (-)
29228742 .. 29242363
13622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG182800.1

Sequence Viewer

Length: 4872 bp
ATGCCACCTAACACCTCATACCGCAAAACCGGTGAGTGTCCGCCTGAGAAGAAGAGCTTAAAAAACCGGTGCCCCTCTCTCTCTTTCCCCCTTCCCCATTTCTCTCGCCATTTCCGTCTCTCTCTCCACTGGTCCGGTCTCTCCGAAACCAAAACCATGTCAAAACCAAACCCTAAATCCCCTTACAATTCCCGCATAGCTCAGAACCAACTGATCGACTCCCTCACCTCCCACATCTCCCTCTACAATTCCCACTCCCATTCCCCAGACCCCAAGCCCAACCCTAACCCTAGGTCCTCCATCCTCAAATGGTTCTCCTCTCTCTCCCTCCACCACCGCCAAGCTCACCTCACCGCCGTCGATCCCAAATTCGTCCGCCTCCTCCTCCAAATGCTCCGCAAGCTCCGCACGCAGGGCCACGGCTCCTTCATCATCCTCCCCGACCTCCCCTCCGGCGACCTCCCCACGCTCTGTTTCAAGAGGTCCGGCGGGCTTTTGTCTAGGGTTGCCGAGTCGAACGAGTCGGAGAAGATGGTGTTCGAGTCGACTAGGTTGTTTTCGTCAAGGGAGGGAGAGAAGGTCGAGGAGTGTTCGTGTTCGGTGAGGGAGATTGATACGGTGACGGTGAGTGAGGGATTGGTTGAGAATTTAGATAGGTTTGTGGAGGCTATGGATCAAATATCAAATGGGGGATTCCTGAGAGGTGAAGACAGTGATTTGGGGTCGGATTGGGTGGAATTGAACTGGTTGAAGGACAAAGGGTATTACAGTATGGAAGCTTTCGTGGCGAACTGGTTAGAGGTTGCTTTGAGGCTTGCTTGGTTGAATTCGAACAATGCAAGGAAAAGAGGGGTGAAGTTGAAAGAGAAGATCAGCGCGGCGGGCGTGGCGGCCAATGTGTATTGGAGGAAGAAGGGGTGTGTGGACTGGTGGGGGAATTTGGATGATGCGATGCGGAGCAATGTAGTGACTTCAGTGTTGGGGAAAGCAGCGAAACCGTTGATTCATGAGATTCTGAAGGGAACAAGTAGTGGGGTAGAGGATGAAATGTGGCTCGCCAACACAGGAATGGAGCAACCATTGAGGTACAACCATAATGTATCTATGCGCAAGACTGTTCCAAAACTTGTGGCTGACACAGAATTTGGGTCAAGCATTATCCCAGCTTCTCTTTCTGGGAAACCTGCTTCCTTAGCCGATGCTTTTAATAATCTCTTTGTGCTTCAGGATATCATAACGATGATATCATTATATCGCCATAATGAATACGATAAAGGGAAATTCTTCTATAGCACATTGAGTTCAATTTCTACCATTTCTGATTTTATACTAAGAAAAATACGAGGATTTCTCATGGTTCTTTTGCTTGACTGCACAAAACTTGAACTTTTATCAGAGGGAAATGAAAAATGCTTACCTAAGAAACCCAAAGCAAAGCCTAGTGCTTCTAGCCGTAAAAGTAAGGGGAGGTCCCACAATTTGAAAAGGCCAAATCCTGTTCCAAGGTCCTGTACAGATGAATTTTTGTGTGAAACATCTGCCAAGGATCTTAGTACATTGGCTTATAAAGAGAAGGCAGATTCAGTGGAGTCCAAGACGACGCATACTATACATCAAGAGAAGGAGATTTTCAGAGAGTCGTCATCATCAAAAGATGAAATGGAACATGCACAAGCATTGGCTGTTGGAAAAGTTCAGACAGCTGCGAGGAAGGGTAGGAAAGGAAAAGGCAAAAAGAAAATAACTGGCTTGAGGAGTGCTAATGATATGGAGAAATTTGATCGATCATTCATGGAAGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAGTCATTCTTGAAGATGACGCTGCAAAGTCTGATCAGGTTTCTGGTGATACAGCCTACCAGAACATCAGATGTGATAATTCAGCTAGTTGTAACATCCCTTTGTCAAATTCAATTCCTTCTGGTTCTGCGAATGGACCTCTTAAAGATGAAGATGCCACCAAAAGCATTCAAGAGAATGATGGTATTGGTTCTAGTGCCACTTTCTGTCATAAAGTTCTGGAAAGTTACCAGTGTTTAAATAATAATAATACTGAAATCCAAACGAAATATTCTGGATCAGAAACTGAAGCCTGTAAGGTAGATGGAAACTTGATAAAGTCTTCAGTGCCTGAGGTAGATGATAAAGTTTCCCATCCTAAAGATATTGACTTGCAGGACAAGCGTGTTGTTAAGTCAGGTGCGAAAGAAGTTTTGCCAGCCAAAGAAATAAGAGCTTCTGATGTAAACGAGGAAACTGTTCTGTTGCAGGACCAAGAGAGTGGAAATAATATATATCACACTAGGGCTCCACGTTCTTTTGATTGCCCTCCTTATGAGTGGCCGGGTGTAGCTTGTGCCTATTTTCCACCTGTTAACTCACATCTCCCACCTGCCACCGATCGATTGCATCTGGATGTTGGTCATAACTGGCAGAATCACCGCCAGTCTTTTCTACCCACAATACATCAGGTGAGAAACTCGGCAATTGAAGGTGGGTGTAATCCAGTTCTGACTCGACCATTGCCAATGAGTATAGATTGGCCCCCAATGGTTCGAAGTGCTCGTAGAGTGGCTCCATACCGGACCTGTACTTATGATTCTGGGTTCGTCTCAAAAAAGCAGTTTACTTATCCACAGGGTTTTGCACACACTGTCCAAATAAATGCAACAACCATGGATGATGAAAGGAAGTATTCTTGGGATTGCGCAGACCTACCTGATCCAACAAAGGCATATGAACTAGTGGATGAATGTGACAGTCACTGGATATCTGAGGATGAAGTTGAGGTGCAAGCATTTTCTGGGGCAGATTATAATCAATACTTTGGTGGTGGTGTGATGTACTGGAATCCTTCTGATCATACCGGGACAGTTTTCTCTCGCCCTCCTTCCCTTAGTTCTGATGATAGCTCATGGGCTTGGCGTGAAGCTGACATAAATAGGGCTGTTGATGATATGGTTGCATTTTCTTCTCCATACAGTACAAATGGTTTGACTTCACCTACTGCTTCCTTCTGTTCTCCTTTTGATCCTTTGGGATCAGGAAACCAGCCTCTTGGATATGTTATGTCAGGAAATGAAGTACCAGGCAAGGTGCTGCATCCCTCATCAACAATGGGAGACACAGTAGTAGATGAGGAATCCTCTGGATCTTTGGCTGACGTAACTGGTGATATTGAAGGGAAGACAGGCGATTCATTGCCTTACCCCATTTTGCGGCCAATCATCATATCAAGATCAAGAGACTTTAAGCGCAGCCATGATCATAAAAGCCCATGTGTTCCTCCTACTATGCGCGATCGACCTAGGATAAAGCGGCCACCGTCGCCGGTGGTGCTTTCTGTCCCACGCGCTCCACGACCACCTCCACCATCTTCTGTGAGTGACTCGAGGAAACACAGGGGATTTCCAACTGTTCGTTCTGGTAGCTCTAGCCCAAGGCACTGGGGTATGAGAGGTTGGTTCCATGATGGAGCTAACTTGGAGGAAGCTTGTTTACGCATGGATGGTGCTGAAGTTGTTTGGCCATTTAGAAATAATATTTCAGGCCGCCCATTGATTCAGCCTCTTCCTGCACCTCTGTTGCAGGATCGACTGATTGCTATTTCTCAACTAGCACGTGATCAGGAACATCCGGATGTTGCATTTCCCCTTCAGCCGCCTGAGTTACATAACTGTCCAACACGGAAGGCATCTCTCTCTCTGATGCACAGCCTTGTGCACGATGAGATTGAATTTTTTTGCAAGAAGGTTGCTACAGAGAATATGTCTCGGAAGCCCTACATCAATTGGGCTGTCAAACGAGTTACACGGTCTCTCCAGGTGCTTTGGCCCAGATCCAGGACAAATATATTTGGTTCAGTTGCAAACGGTTTGTCCCTTCCAACTAGTGATGTGGACCTTGTGGTTTGTCTACCTCCTGTTAGGAATTTGGAACCCATTAAAGAAGCTGGGATATTGGAGGGTCGAAATGGTATTAAAGAGACATGCCTTCAGCATGCTGCCAGGTATCTTGTCAATCAGGATTGGGTTAAAAATGATTCTCTTAAGACCGTGGAAAATACAGCTATACCGATTATAATGCTTGTGGTGGAAGTTCCCAATGATCTAATTGCCTCGTCTTCGTCTAACGTGCAATCACCAAAAGAAGAGGCACCTCACAGTACTGGTGAGCCAGACTGTAATGTGCACTCCAGTGGGGTTATTTTAGAAGAATCTGCTATGCCTAAGTGCCCCCAGATAACTTATGATGCTACAAAGGATTCAGTATCAATTCGCATTGACATCAGTTTCAAATCTCCATCTCATACAGGATTTCAAACTACACAGCTGGTTAAAGATTTAACTGAGCAGTTTCCAGCCGCTACACCCCTTGCTTTGGTACTGAAACAGTTCTTGGCTGATCGTAGTCTGGATCAGTCTTATTCTGGTGGCTTGAGTTCCTACTGCTTGGTACTACTAATTGTGCGTTTTCTCCAGCATGAGTATCATCTTAGCCGACCTATCAACCAAAACTTTGGAAACCTTCTGATGAATTTTCTTTATTTTTTTGGGAATGTTTTTGATCCTCGGCAAATGCGTATTTCCGTACAGGGAAGTGGTGTTTATATAAAGAGGGAAAGAGGTTGCAGCATCGACCCCATACATATTGATGATCCCCTTTTTCCAACTAATAATGTCGGACGCAATTGCTTTCGGATACATCAGTGTATCAAGGCGTTTTCAGAAGCTTATTCTATTTTGGAGAATGAGCTTGCTTCTCTTCCTAATGATGATGATGCATCTTCAAGTCCCCCATATAGACTGCTTCCTAAAATCATCCCGAGTATTGATCTAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

1623

Amino Acids

180.44

Weight (kDa)

7.04

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 1275 - 1371 1.5e-07 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1566, 2844, 4109
AarI CACCTGC 1 cut(s) 2428
Acc16I TGCGCA 2 cut(s) 1109, 2737
Acc36I ACCTGC 2 cut(s) 1192, 2428
AccB1I GGYRCC 2 cut(s) 69, 4183
AccI GTMKAC 2 cut(s) 545, 3943
AccII CGCG 3 cut(s) 878, 3327, 3381
AccIII TCCGGA 1 cut(s) 3664
AcoI YGGCCR 5 cut(s) 891, 2369, 3248, 3347, 3554
AcuI CTGAAG 9 cut(s) 957, 1037, 1208, 1812, 2136, 2136, 3564, 3668, 4007
AcvI CACGTG 1 cut(s) 3650
AfiI CCNNNNNNNGG 4 cut(s) 412, 3246, 3870, 4408
AflII CTTAAG 1 cut(s) 4076
AgeI ACCGGT 2 cut(s) 29, 66
AhlI ACTAGT 2 cut(s) 2770, 3917
AjnI CCWGG 4 cut(s) 3115, 3849, 3869, 4034
AjuI GAANNNNNNNTTGG 4 cut(s) 3433, 3465, 4409, 4441
AleI CACNNNNGTG 1 cut(s) 4224
Alw21I GWGCWC 3 cut(s) 2593, 3753, 4221
Alw26I GTCTC 8 cut(s) 122, 143, 2644, 3144, 3267, 3804, 3849, 4007
Alw44I GTGCAC 2 cut(s) 3749, 4217
AlwNI CAGNNNCTG 3 cut(s) 2114, 2159, 2793
Ama87I CYCGRG 2 cut(s) 3418, 4852
Aor13HI TCCGGA 1 cut(s) 3664
ApaLI GTGCAC 2 cut(s) 3749, 4217
ApeKI GCWGC 7 cut(s) 989, 1703, 1850, 3127, 3285, 4031, 4659
ArsI GACNNNNNNTTYG 4 cut(s) 1127, 1159, 3890, 3922
AsiGI ACCGGT 2 cut(s) 29, 66
Asp700I GAANNNNTTC 3 cut(s) 1283, 2286, 4421
AspA2I CCTAGG 2 cut(s) 290, 3335
AspLEI GCGC 6 cut(s) 878, 1110, 2738, 3285, 3327, 3383
AsuC2I CCSGG 2 cut(s) 2373, 2896
AsuII TTCGAA 2 cut(s) 830, 2584
AvaI CYCGRG 2 cut(s) 3418, 4852
AvaII GGWCC 9 cut(s) 132, 294, 483, 1470, 1506, 1964, 2299, 2613, 3928
AvrII CCTAGG 2 cut(s) 290, 3335
AxyI CCTNAGG 1 cut(s) 2160
BaeGI GKGCMC 4 cut(s) 74, 3753, 4221, 4265
BaeI ACNNNNGTAYC 2 cut(s) 4721, 4754
BalI TGGCCA 1 cut(s) 3556
BanI GGYRCC 2 cut(s) 69, 4183
BanII GRGCYC 1 cut(s) 2338
BarI GAAGNNNNNNTAC 2 cut(s) 3510, 3542
BbrPI CACGTG 1 cut(s) 3650
BbsI GAAGAC 4 cut(s) 714, 2142, 3221, 4143
Bbv12I GWGCWC 3 cut(s) 2593, 3753, 4221
BbvI GCAGC 7 cut(s) 1001, 1690, 1837, 3114, 3297, 4018, 4671
BccI CCATC 9 cut(s) 308, 526, 2003, 2126, 2190, 3409, 3494, 3530, 4339
BceAI ACGGC 3 cut(s) 341, 436, 1437
BcgI CGANNNNNNTGC 2 cut(s) 4093, 4127
BciT130I CCWGG 4 cut(s) 3117, 3851, 3871, 4036
BciVI GTATCC 1 cut(s) 4722
BclI TGATCA 4 cut(s) 1861, 2887, 3292, 3652
BcnI CCSGG 2 cut(s) 2373, 2896
BcoDI GTCTC 8 cut(s) 122, 143, 2644, 3144, 3267, 3804, 3849, 4007
BcuI ACTAGT 2 cut(s) 2770, 3917
BfmI CTRYAG 2 cut(s) 1288, 3786
BfrI CTTAAG 1 cut(s) 4076
BfuAI ACCTGC 2 cut(s) 1192, 2428
BfuI GTATCC 1 cut(s) 4722
BlnI CCTAGG 2 cut(s) 290, 3335
BmcAI AGTACT 1 cut(s) 4195
Bme1390I CCNGG 6 cut(s) 2373, 2896, 3117, 3851, 3871, 4036
Bme18I GGWCC 9 cut(s) 132, 294, 483, 1470, 1506, 1964, 2299, 2613, 3928
BmeT110I CYCGRG 2 cut(s) 3418, 4852
BmiI GGNNCC 9 cut(s) 71, 424, 1472, 2337, 2573, 2604, 3494, 3966, 4185
BmrFI CCNGG 6 cut(s) 2373, 2896, 3117, 3851, 3871, 4036
BmrI ACTGGG 1 cut(s) 3484
BmuI ACTGGG 1 cut(s) 3484
BpiI GAAGAC 4 cut(s) 714, 2142, 3221, 4143
BplI GAGNNNNNCTC 4 cut(s) 1335, 1367, 3158, 3190
BpmI CTGGAG 3 cut(s) 3833, 4207, 4490
Bpu10I CCTNAGC 1 cut(s) 1192
Bpu14I TTCGAA 2 cut(s) 830, 2584
BpuEI CTTGAG 2 cut(s) 1771, 4486
BpuMI CCSGG 2 cut(s) 2373, 2896
Bsa29I ATCGAT 2 cut(s) 1783, 2431
BsaAI YACGTR 1 cut(s) 3650
BsaBI GATNNNNATC 1 cut(s) 2844
BsaI GGTCTC 2 cut(s) 143, 3849
BsaJI CCNNGG 9 cut(s) 290, 418, 1502, 1542, 2703, 3335, 3467, 4083, 4598
BsaWI WCCGGW 5 cut(s) 29, 66, 134, 2610, 3664
Bsc4I CCNNNNNNNGG 4 cut(s) 412, 3246, 3870, 4408
Bse118I RCCGGY 3 cut(s) 29, 66, 3358
Bse21I CCTNAGG 1 cut(s) 2160
Bse3DI GCAATG 3 cut(s) 967, 2549, 3227
Bse8I GATNNNNATC 1 cut(s) 2844
BseAI TCCGGA 1 cut(s) 3664
BseBI CCWGG 4 cut(s) 3117, 3851, 3871, 4036
BseCI ATCGAT 2 cut(s) 1783, 2431
BseDI CCNNGG 9 cut(s) 290, 418, 1502, 1542, 2703, 3335, 3467, 4083, 4598
BseJI GATNNNNATC 1 cut(s) 2844
BseLI CCNNNNNNNGG 4 cut(s) 412, 3246, 3870, 4408
BseMI GCAATG 3 cut(s) 967, 2549, 3227
BseMII CTCAG 7 cut(s) 36, 215, 689, 2151, 2793, 3684, 4368
BseRI GAGGAG 5 cut(s) 307, 371, 374, 599, 1768
BseSI GKGCMC 4 cut(s) 74, 3753, 4221, 4265
BseXI GCAGC 7 cut(s) 1001, 1690, 1837, 3114, 3297, 4018, 4671
BseYI CCCAGC 2 cut(s) 1162, 3980
BsgI GTGCAG 2 cut(s) 1357, 3588
Bsh1236I CGCG 3 cut(s) 878, 3327, 3381
Bsh1285I CGRYCG 2 cut(s) 2431, 3331
BshNI GGYRCC 2 cut(s) 69, 4183
BshTI ACCGGT 2 cut(s) 29, 66
BshVI ATCGAT 2 cut(s) 1783, 2431
BsiEI CGRYCG 2 cut(s) 2431, 3331
BsiHKAI GWGCWC 3 cut(s) 2593, 3753, 4221
BsiHKCI CYCGRG 2 cut(s) 3418, 4852
BslFI GGGAC 5 cut(s) 1456, 2911, 3359, 3892, 4806
BslI CCNNNNNNNGG 4 cut(s) 412, 3246, 3870, 4408
BsmAI GTCTC 8 cut(s) 122, 143, 2644, 3144, 3267, 3804, 3849, 4007
BsmBI CGTCTC 2 cut(s) 122, 2644
BsmFI GGGAC 5 cut(s) 1456, 2911, 3359, 3892, 4806
BsmI GAATGC 1 cut(s) 1995
Bso31I GGTCTC 2 cut(s) 143, 3849
BsoBI CYCGRG 2 cut(s) 3418, 4852
Bsp119I TTCGAA 2 cut(s) 830, 2584
Bsp1286I GDGCHC 6 cut(s) 74, 2338, 2593, 3753, 4221, 4265
Bsp13I TCCGGA 1 cut(s) 3664
Bsp1407I TGTACA 1 cut(s) 1511
Bsp19I CCATGG 1 cut(s) 2703
BspCNI CTCAG 7 cut(s) 37, 214, 690, 2152, 2794, 3685, 4369
BspDI ATCGAT 2 cut(s) 1783, 2431
BspEI TCCGGA 1 cut(s) 3664
BspFNI CGCG 3 cut(s) 878, 3327, 3381
BspHI TCATGA 1 cut(s) 1006
BspLI GGNNCC 9 cut(s) 71, 424, 1472, 2337, 2573, 2604, 3494, 3966, 4185
BspMI ACCTGC 2 cut(s) 1192, 2428
BspQI GCTCTTC 1 cut(s) 47
BspT104I TTCGAA 2 cut(s) 830, 2584
BspT107I GGYRCC 2 cut(s) 69, 4183
BspTI CTTAAG 1 cut(s) 4076
BspTNI GGTCTC 2 cut(s) 143, 3849
BsrDI GCAATG 3 cut(s) 967, 2549, 3227
BsrFI RCCGGY 3 cut(s) 29, 66, 3358
BsrGI TGTACA 1 cut(s) 1511
BssAI RCCGGY 3 cut(s) 29, 66, 3358
BssECI CCNNGG 9 cut(s) 290, 418, 1502, 1542, 2703, 3335, 3467, 4083, 4598
BssT1I CCWWGG 6 cut(s) 290, 1502, 1542, 2703, 3335, 3467
Bst2UI CCWGG 4 cut(s) 3117, 3851, 3871, 4036
Bst6I CTCTTC 4 cut(s) 47, 3603, 4173, 4797
BstAFI CTTAAG 1 cut(s) 4076
BstAUI TGTACA 1 cut(s) 1511
BstBAI YACGTR 1 cut(s) 3650
BstBI TTCGAA 2 cut(s) 830, 2584
BstDSI CCRYGG 3 cut(s) 418, 2703, 4083
BstFNI CGCG 3 cut(s) 878, 3327, 3381
BstHHI GCGC 6 cut(s) 878, 1110, 2738, 3285, 3327, 3383
BstMAI GTCTC 8 cut(s) 122, 143, 2644, 3144, 3267, 3804, 3849, 4007
BstMCI CGRYCG 2 cut(s) 2431, 3331
BstNI CCWGG 4 cut(s) 3117, 3851, 3871, 4036
BstNSI RCATGY 3 cut(s) 1670, 4020, 4031
BstSCI CCNGG 6 cut(s) 2371, 2894, 3115, 3849, 3869, 4034
BstSFI CTRYAG 2 cut(s) 1288, 3786
BstSLI GKGCMC 4 cut(s) 74, 3753, 4221, 4265
BstUI CGCG 3 cut(s) 878, 3327, 3381
BstV1I GCAGC 7 cut(s) 1001, 1690, 1837, 3114, 3297, 4018, 4671
BstV2I GAAGAC 4 cut(s) 714, 2142, 3221, 4143
BstX2I RGATCY 3 cut(s) 1546, 3179, 3866
BstXI CCANNNNNNTGG 4 cut(s) 2309, 3086, 3474, 4547
BstYI RGATCY 3 cut(s) 1546, 3179, 3866
Bsu15I ATCGAT 2 cut(s) 1783, 2431
Bsu36I CCTNAGG 1 cut(s) 2160
BsuI GTATCC 1 cut(s) 4722
BsuTUI ATCGAT 2 cut(s) 1783, 2431
BtgI CCRYGG 3 cut(s) 418, 2703, 4083
BtgZI GCGATG 1 cut(s) 965
BveI ACCTGC 2 cut(s) 1192, 2428
CaiI CAGNNNCTG 3 cut(s) 2114, 2159, 2793
CciI TCATGA 1 cut(s) 1006
CfoI GCGC 6 cut(s) 878, 1110, 2738, 3285, 3327, 3383
Cfr10I RCCGGY 3 cut(s) 29, 66, 3358
ClaI ATCGAT 2 cut(s) 1783, 2431
CseI GACGC 3 cut(s) 1609, 1856, 4722
CspAI ACCGGT 2 cut(s) 29, 66
CspCI CAANNNNNGTGG 2 cut(s) 1110, 1145
DraI TTTAAA 1 cut(s) 2067
EaeI YGGCCR 5 cut(s) 891, 2369, 3248, 3347, 3554
Eam1104I CTCTTC 4 cut(s) 47, 3603, 4173, 4797
EarI CTCTTC 4 cut(s) 47, 3603, 4173, 4797
EciI GGCGGA 2 cut(s) 30, 365
Eco130I CCWWGG 6 cut(s) 290, 1502, 1542, 2703, 3335, 3467
Eco24I GRGCYC 1 cut(s) 2338
Eco31I GGTCTC 2 cut(s) 143, 3849
Eco32I GATATC 3 cut(s) 1231, 1245, 2799
Eco47I GGWCC 9 cut(s) 132, 294, 483, 1470, 1506, 1964, 2299, 2613, 3928
Eco57I CTGAAG 9 cut(s) 957, 1037, 1208, 1812, 2136, 2136, 3564, 3668, 4007
Eco72I CACGTG 1 cut(s) 3650
Eco81I CCTNAGG 1 cut(s) 2160
Eco88I CYCGRG 2 cut(s) 3418, 4852
EcoO109I RGGNCCY 3 cut(s) 294, 1470, 1506
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 4 cut(s) 3115, 3849, 3869, 4034
EcoRV GATATC 3 cut(s) 1231, 1245, 2799
EcoT14I CCWWGG 6 cut(s) 290, 1502, 1542, 2703, 3335, 3467
EcoT22I ATGCAT 1 cut(s) 4813
EcoT38I GRGCYC 1 cut(s) 2338
ErhI CCWWGG 6 cut(s) 290, 1502, 1542, 2703, 3335, 3467
Esp3I CGTCTC 2 cut(s) 122, 2644
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 5 cut(s) 1456, 2911, 3359, 3892, 4806
FauI CCCGC 3 cut(s) 200, 482, 874
FauNDI CATATG 1 cut(s) 2764
FbaI TGATCA 4 cut(s) 1861, 2887, 3292, 3652
FblI GTMKAC 2 cut(s) 545, 3943
FriOI GRGCYC 1 cut(s) 2338
FspI TGCGCA 2 cut(s) 1109, 2737
GlaI GCGC 6 cut(s) 877, 1109, 2737, 3284, 3326, 3382
GsaI CCCAGC 2 cut(s) 1166, 3984
GsuI CTGGAG 3 cut(s) 3833, 4207, 4490
HgaI GACGC 3 cut(s) 1609, 1856, 4722
HhaI GCGC 6 cut(s) 878, 1110, 2738, 3285, 3327, 3383
Hin6I GCGC 6 cut(s) 876, 1108, 2736, 3283, 3325, 3381
HinP1I GCGC 6 cut(s) 876, 1108, 2736, 3283, 3325, 3381
HincII GTYRAC 2 cut(s) 546, 2404
HindII GTYRAC 2 cut(s) 546, 2404
HindIII AAGCTT 4 cut(s) 777, 1797, 3519, 4758
HpaI GTTAAC 1 cut(s) 2404
Hpy99I CGWCG 3 cut(s) 362, 1603, 3358
HpyCH4IV ACGT 4 cut(s) 2341, 3192, 3649, 4161
HpySE526I ACGT 4 cut(s) 2341, 3192, 3649, 4161
HspAI GCGC 6 cut(s) 876, 1108, 2736, 3283, 3325, 3381
Kpn2I TCCGGA 1 cut(s) 3664
Ksp22I TGATCA 4 cut(s) 1861, 2887, 3292, 3652
KspAI GTTAAC 1 cut(s) 2404
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 9 cut(s) 399, 408, 428, 957, 1072, 2341, 2608, 3388, 3503
Lsp1109I GCAGC 7 cut(s) 1001, 1690, 1837, 3114, 3297, 4018, 4671
MaeII ACGT 4 cut(s) 2341, 3192, 3649, 4161
MfeI CAATTG 3 cut(s) 2514, 3817, 4717
MflI RGATCY 3 cut(s) 1546, 3179, 3866
MhlI GDGCHC 6 cut(s) 74, 2338, 2593, 3753, 4221, 4265
MlsI TGGCCA 1 cut(s) 3556
MluNI TGGCCA 1 cut(s) 3556
MlyI GAGTC 8 cut(s) 212, 521, 530, 551, 1598, 1646, 2536, 3410
MmeI TCCRAC 9 cut(s) 504, 705, 1666, 2777, 3464, 3734, 3938, 4690, 4721
Mox20I TGGCCA 1 cut(s) 3556
Mph1103I ATGCAT 1 cut(s) 4813
MroI TCCGGA 1 cut(s) 3664
MroXI GAANNNNTTC 3 cut(s) 1283, 2286, 4421
MscI TGGCCA 1 cut(s) 3556
MslI CAYNNNNRTG 6 cut(s) 1067, 1238, 2442, 3666, 4224, 4680
Msp20I TGGCCA 1 cut(s) 3556
MspA1I CMGCKG 2 cut(s) 1703, 4360
MspCI CTTAAG 1 cut(s) 4076
MspR9I CCNGG 6 cut(s) 2373, 2896, 3117, 3851, 3871, 4036
MunI CAATTG 3 cut(s) 2514, 3817, 4717
Mva1269I GAATGC 1 cut(s) 1995
MvaI CCWGG 4 cut(s) 3117, 3851, 3871, 4036
MvnI CGCG 3 cut(s) 878, 3327, 3381
NciI CCSGG 2 cut(s) 2373, 2896
NcoI CCATGG 1 cut(s) 2703
NdeI CATATG 1 cut(s) 2764
NlaIV GGNNCC 9 cut(s) 71, 424, 1472, 2337, 2573, 2604, 3494, 3966, 4185
NmeAIII GCCGAG 3 cut(s) 535, 2489, 4579
NmuCI GTSAC 5 cut(s) 619, 967, 2783, 2789, 3413
NsbI TGCGCA 2 cut(s) 1109, 2737
NsiI ATGCAT 1 cut(s) 4813
NspI RCATGY 3 cut(s) 1670, 4020, 4031
NspV TTCGAA 2 cut(s) 830, 2584
OliI CACNNNNGTG 1 cut(s) 4224
PaeI GCATGC 1 cut(s) 4031
PaeR7I CTCGAG 1 cut(s) 3418
PagI TCATGA 1 cut(s) 1006
PaqCI CACCTGC 1 cut(s) 2428
PciSI GCTCTTC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 4 cut(s) 112, 2590, 3385, 4606
PctI GAATGC 1 cut(s) 1995
PdmI GAANNNNTTC 3 cut(s) 1283, 2286, 4421
PfoI TCCNGGA 1 cut(s) 3869
PinAI ACCGGT 2 cut(s) 29, 66
Ple19I CGATCG 2 cut(s) 2431, 3331
PleI GAGTC 8 cut(s) 212, 520, 529, 550, 1597, 1645, 2536, 3410
PmaCI CACGTG 1 cut(s) 3650
PmlI CACGTG 1 cut(s) 3650
PpsI GAGTC 8 cut(s) 212, 520, 529, 550, 1597, 1645, 2536, 3410
Ppu21I YACGTR 1 cut(s) 3650
PpuMI RGGWCCY 3 cut(s) 294, 1470, 1506
PsiI TTATAA 3 cut(s) 1566, 2844, 4109
Psp5II RGGWCCY 3 cut(s) 294, 1470, 1506
Psp6I CCWGG 4 cut(s) 3115, 3849, 3869, 4034
PspCI CACGTG 1 cut(s) 3650
PspFI CCCAGC 2 cut(s) 1162, 3980
PspGI CCWGG 4 cut(s) 3115, 3849, 3869, 4034
PspN4I GGNNCC 9 cut(s) 71, 424, 1472, 2337, 2573, 2604, 3494, 3966, 4185
PspPPI RGGWCCY 3 cut(s) 294, 1470, 1506
PspXI VCTCGAGB 1 cut(s) 3418
PstNI CAGNNNCTG 3 cut(s) 2114, 2159, 2793
PsuI RGATCY 3 cut(s) 1546, 3179, 3866
PvuI CGATCG 2 cut(s) 2431, 3331
PvuII CAGCTG 2 cut(s) 1703, 4360
RseI CAYNNNNRTG 6 cut(s) 1067, 1238, 2442, 3666, 4224, 4680
SalI GTCGAC 1 cut(s) 544
SapI GCTCTTC 1 cut(s) 47
ScaI AGTACT 1 cut(s) 4195
SchI GAGTC 8 cut(s) 212, 521, 530, 551, 1598, 1646, 2536, 3410
ScrFI CCNGG 6 cut(s) 2373, 2896, 3117, 3851, 3871, 4036
SduI GDGCHC 6 cut(s) 74, 2338, 2593, 3753, 4221, 4265
SfcI CTRYAG 2 cut(s) 1288, 3786
Sfr274I CTCGAG 1 cut(s) 3418
SfuI TTCGAA 2 cut(s) 830, 2584
SgrAI CRCCGGYG 1 cut(s) 3358
SinI GGWCC 9 cut(s) 132, 294, 483, 1470, 1506, 1964, 2299, 2613, 3928
SlaI CTCGAG 1 cut(s) 3418
SmiMI CAYNNNNRTG 6 cut(s) 1067, 1238, 2442, 3666, 4224, 4680
SmlI CTYRAG 4 cut(s) 1750, 3418, 4076, 4465
SmoI CTYRAG 4 cut(s) 1750, 3418, 4076, 4465
SpeI ACTAGT 2 cut(s) 2770, 3917
SphI GCATGC 1 cut(s) 4031
SspI AATATT 2 cut(s) 2099, 3571
StyD4I CCNGG 6 cut(s) 2371, 2894, 3115, 3849, 3869, 4034
StyI CCWWGG 6 cut(s) 290, 1502, 1542, 2703, 3335, 3467
TaiI ACGT 4 cut(s) 2344, 3195, 3652, 4164
TatI WGTACW 6 cut(s) 1511, 1553, 2618, 2871, 3011, 4193
TauI GCSGC 7 cut(s) 881, 893, 3250, 3349, 3582, 3691, 4394
TseFI GTSAC 5 cut(s) 619, 967, 2783, 2789, 3413
TseI GCWGC 7 cut(s) 989, 1703, 1850, 3127, 3285, 4031, 4659
Tsp45I GTSAC 5 cut(s) 619, 967, 2783, 2789, 3413
TspGWI ACGGA 3 cut(s) 104, 3730, 4606
Vha464I CTTAAG 1 cut(s) 4076
VneI GTGCAC 2 cut(s) 3749, 4217
VpaK11BI GGWCC 9 cut(s) 132, 294, 483, 1470, 1506, 1964, 2299, 2613, 3928
XceI RCATGY 3 cut(s) 1670, 4020, 4031
XcmI CCANNNNNNNNNTGG 1 cut(s) 1066
XhoI CTCGAG 1 cut(s) 3418
XmaJI CCTAGG 2 cut(s) 290, 3335
XmiI GTMKAC 2 cut(s) 545, 3943
XmnI GAANNNNTTC 3 cut(s) 1283, 2286, 4421
ZrmI AGTACT 1 cut(s) 4195
Zsp2I ATGCAT 1 cut(s) 4813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.