AT4G00220

lob domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014318
Physical Location & Seq
Forward (+)
89908 .. 92255
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00220.1

Sequence Viewer

Length: 687 bp
ATGAGCAGTAGCGGAAACCCTAGCAGCAGCAGCGGTGGAGGAGGAGGACCGTGCGGCGCGTGCAAGTTCTTGAGAAGGAAGTGTGTCGCTGGCTGCATCTTCGCTCCTTACTTTGACTCCGAGCAAGGAGCGGCGCACTTCGCGGCGGTTCACAAGGTGTTCGGAGCGAGCAACGTCTCCAAACTCCTCCACCATGTTCCCGAGCATAAACGACCAGACGCCGTCGTTTCAATCTGCTTTGAGGCTCAAGCTCGTCTCAGAGACCCTATCTACGGCTGCGTCTCTCACATCGTCTCTCTTCAGCAACAGGTGGTAAGTTTGCAAACTGAGCTTTCATATCTACAAGCACACTTAGCAACTCTAGAGCTGCCACAACCTCAACCACCACAGGTTCCGGTGTCATCTTCAGGATCTCTACAGGCTCTTTCCATAACGGACCTCCCAACAATATCACCGTCGGTGTACGACCTCTCCTCCATCTTCGAGCCGGTCATGTCCTCCACTTGGGCCATGCAGCAACAACCACGACCGTCTGATCATCTCTTCGGCGTCCCGTCATCGTCCAATATGGGCGGAGGCGGTGAGCTCCAAGCACTGGCGCGTGAGTTTATTCACGGTGGGCAAATGCCAGCTCAGCCATCGCCGGGAACCAGTGGTTCTGCCTCGTCAGTGATAAAACGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009653 GO:0009888 GO:0010087 GO:0010089 GO:0010199 GO:0032502 GO:0048646 GO:0048856 GO:0048859 GO:0090691
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

228

Amino Acids

23.97

Weight (kDa)

6.74

Isoelectric Point (pI)

74.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 17 - 115 9.3e-37 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45420 AT4G00220
fragaria_vesca FvH4_7g11990
malus_domestica MD02G1202500.v1.1
prunus_persica Prupe.2G146600_v2.0.a1
pyrus_communis pycom02g16570
rosa_chinensis RchiOBHm_Chr1g0350711
rosa_laevigata RLG00000028461
rosa_multiflora Rmu_sc0002150.1_g000005 Rmu_sc0002150.1_g000006
rosa_roxburghii Rroxscaffold_4G00304580
rosa_rugosa Rorug01G0213300
rosa_samantha Rh1AG227600 Rh1BG197100 Rh1CG212500 Rh1DG225000
rosa_wichuraiana Rw1G019710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 595
AccBSI CCGCTC 1 cut(s) 131
AccII CGCG 3 cut(s) 59, 143, 601
AciI CCGC 8 cut(s) 12, 33, 54, 131, 143, 146, 573, 579
AclWI GGATC 1 cut(s) 418
AcuI CTGAAG 2 cut(s) 284, 390
AcyI GRCGYC 2 cut(s) 219, 549
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 4 cut(s) 272, 504, 595, 644
AgsI TTSAA 1 cut(s) 231
AluBI AGCT 5 cut(s) 251, 331, 367, 586, 632
AluI AGCT 5 cut(s) 251, 331, 367, 586, 632
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 5 cut(s) 181, 255, 260, 286, 298
AlwI GGATC 1 cut(s) 418
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 1 cut(s) 507
ApeKI GCWGC 7 cut(s) 24, 27, 30, 93, 276, 367, 514
AspLEI GCGC 3 cut(s) 59, 136, 601
AspS9I GGNCC 3 cut(s) 47, 436, 507
AsuC2I CCSGG 1 cut(s) 645
AsuHPI GGTGA 2 cut(s) 444, 593
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 2 cut(s) 47, 436
BanII GRGCYC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 7 cut(s) 36, 39, 42, 80, 263, 354, 526
BccI CCATC 2 cut(s) 485, 646
BceAI ACGGC 2 cut(s) 206, 289
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 1 cut(s) 645
BcoDI GTCTC 5 cut(s) 181, 255, 260, 286, 298
BfaI CTAG 2 cut(s) 21, 362
BfmI CTRYAG 1 cut(s) 416
BlpI GCTNAGC 1 cut(s) 633
Bme1390I CCNGG 1 cut(s) 645
Bme18I GGWCC 2 cut(s) 47, 436
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 3 cut(s) 47, 436, 507
BmiI GGNNCC 2 cut(s) 393, 649
BmrFI CCNGG 1 cut(s) 645
BmsI GCATC 1 cut(s) 105
Bpu1102I GCTNAGC 1 cut(s) 633
BpuEI CTTGAG 2 cut(s) 91, 231
BpuMI CCSGG 1 cut(s) 645
BsaHI GRCGYC 2 cut(s) 219, 549
BsaI GGTCTC 1 cut(s) 255
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 455, 458, 485, 488
Bsc4I CCNNNNNNNGG 4 cut(s) 272, 504, 595, 644
Bse118I RCCGGY 1 cut(s) 487
Bse1I ACTGG 2 cut(s) 600, 651
BseLI CCNNNNNNNGG 4 cut(s) 272, 504, 595, 644
BseMII CTCAG 3 cut(s) 271, 318, 647
BseNI ACTGG 2 cut(s) 600, 651
BseRI GAGGAG 4 cut(s) 54, 57, 176, 463
BseXI GCAGC 7 cut(s) 36, 39, 42, 80, 263, 354, 526
Bsh1236I CGCG 3 cut(s) 59, 143, 601
Bsh1285I CGRYCG 1 cut(s) 530
BshFI GGCC 1 cut(s) 509
BsiEI CGRYCG 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 3 cut(s) 395, 488, 644
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 4 cut(s) 272, 504, 595, 644
BsmAI GTCTC 5 cut(s) 181, 255, 260, 286, 298
BsmBI CGTCTC 4 cut(s) 181, 260, 286, 298
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 1 cut(s) 509
Bso31I GGTCTC 1 cut(s) 255
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 588
Bsp143I GATC 2 cut(s) 410, 535
Bsp1720I GCTNAGC 1 cut(s) 633
BspACI CCGC 8 cut(s) 12, 33, 54, 131, 143, 146, 573, 579
BspANI GGCC 1 cut(s) 509
BspCNI CTCAG 3 cut(s) 270, 319, 646
BspFNI CGCG 3 cut(s) 59, 143, 601
BspLI GGNNCC 2 cut(s) 393, 649
BspPI GGATC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 255
BsrBI CCGCTC 1 cut(s) 131
BsrFI RCCGGY 1 cut(s) 487
BsrI ACTGG 2 cut(s) 600, 651
BssAI RCCGGY 1 cut(s) 487
BssMI GATC 2 cut(s) 410, 535
BssNI GRCGYC 2 cut(s) 219, 549
Bst4CI ACNGT 4 cut(s) 51, 456, 531, 617
Bst6I CTCTTC 2 cut(s) 303, 548
BstACI GRCGYC 2 cut(s) 219, 549
BstC8I GCNNGC 4 cut(s) 61, 91, 169, 630
BstDEI CTNAG 4 cut(s) 257, 327, 352, 633
BstFNI CGCG 3 cut(s) 59, 143, 601
BstHHI GCGC 3 cut(s) 59, 136, 601
BstKTI GATC 2 cut(s) 413, 538
BstMAI GTCTC 5 cut(s) 181, 255, 260, 286, 298
BstMBI GATC 2 cut(s) 410, 535
BstMCI CGRYCG 1 cut(s) 530
BstMWI GCNNNNNNNGC 6 cut(s) 30, 60, 140, 328, 353, 634
BstSCI CCNGG 1 cut(s) 643
BstSFI CTRYAG 1 cut(s) 416
BstUI CGCG 3 cut(s) 59, 143, 601
BstV1I GCAGC 7 cut(s) 36, 39, 42, 80, 263, 354, 526
BstX2I RGATCY 1 cut(s) 410
BstYI RGATCY 1 cut(s) 410
BsuRI GGCC 1 cut(s) 509
BtgZI GCGATG 1 cut(s) 624
BtsIMutI CAGTG 3 cut(s) 593, 658, 675
Cac8I GCNNGC 4 cut(s) 61, 91, 169, 630
CfoI GCGC 3 cut(s) 59, 136, 601
Cfr10I RCCGGY 1 cut(s) 487
Cfr13I GGNCC 3 cut(s) 47, 436, 507
CseI GACGC 3 cut(s) 227, 268, 538
Csp6I GTAC 1 cut(s) 463
CviAII CATG 3 cut(s) 194, 493, 511
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 4 cut(s) 257, 327, 352, 633
DpnI GATC 2 cut(s) 412, 537
DpnII GATC 2 cut(s) 410, 535
DraIII CACNNNGTG 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 303, 548
EarI CTCTTC 2 cut(s) 303, 548
EciI GGCGGA 1 cut(s) 588
Ecl136II GAGCTC 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 588
Eco31I GGTCTC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 47, 436
Eco53kI GAGCTC 1 cut(s) 586
Eco57I CTGAAG 2 cut(s) 284, 390
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 588
Esp3I CGTCTC 4 cut(s) 181, 260, 286, 298
FaeI CATG 3 cut(s) 197, 496, 514
FaiI YATR 7 cut(s) 195, 207, 337, 431, 494, 512, 569
FaqI GGGAC 1 cut(s) 536
FatI CATG 3 cut(s) 193, 492, 510
FbaI TGATCA 1 cut(s) 535
FriOI GRGCYC 1 cut(s) 588
FspBI CTAG 2 cut(s) 21, 362
GlaI GCGC 3 cut(s) 58, 135, 600
HaeIII GGCC 1 cut(s) 509
HapII CCGG 3 cut(s) 395, 488, 644
HgaI GACGC 3 cut(s) 227, 268, 538
HhaI GCGC 3 cut(s) 59, 136, 601
Hin1I GRCGYC 2 cut(s) 219, 549
Hin1II CATG 3 cut(s) 197, 496, 514
Hin6I GCGC 3 cut(s) 57, 134, 599
HinP1I GCGC 3 cut(s) 57, 134, 599
HinfI GANTC 1 cut(s) 116
HpaII CCGG 3 cut(s) 395, 488, 644
HphI GGTGA 2 cut(s) 444, 593
Hpy166II GTNNAC 2 cut(s) 151, 463
Hpy188I TCNGA 4 cut(s) 121, 164, 260, 535
Hpy188III TCNNGA 4 cut(s) 70, 200, 362, 408
Hpy8I GTNNAC 2 cut(s) 151, 463
Hpy99I CGWCG 2 cut(s) 227, 460
HpyAV CCTTC 1 cut(s) 69
HpyCH4III ACNGT 4 cut(s) 51, 456, 531, 617
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 63, 96, 322, 514
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 60, 140, 328, 353, 634
HpyF3I CTNAG 4 cut(s) 257, 327, 352, 633
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 219, 549
Hsp92II CATG 3 cut(s) 197, 496, 514
HspAI GCGC 3 cut(s) 57, 134, 599
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 2 cut(s) 410, 535
LmnI GCTCC 4 cut(s) 109, 128, 164, 591
Lsp1109I GCAGC 7 cut(s) 36, 39, 42, 80, 263, 354, 526
LweI GCATC 1 cut(s) 105
MaeI CTAG 2 cut(s) 21, 362
MaeII ACGT 1 cut(s) 174
MalI GATC 2 cut(s) 412, 537
MbiI CCGCTC 1 cut(s) 131
MboI GATC 2 cut(s) 410, 535
MboII GAAGA 5 cut(s) 91, 290, 396, 472, 535
MflI RGATCY 1 cut(s) 410
MhlI GDGCHC 1 cut(s) 588
MlyI GAGTC 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 3 cut(s) 395, 488, 644
MspR9I CCNGG 1 cut(s) 645
MvnI CGCG 3 cut(s) 59, 143, 601
MwoI GCNNNNNNNGC 6 cut(s) 30, 60, 140, 328, 353, 634
NciI CCSGG 1 cut(s) 645
NdeII GATC 2 cut(s) 410, 535
NlaIII CATG 3 cut(s) 197, 496, 514
NlaIV GGNNCC 2 cut(s) 393, 649
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 595
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
Psp124BI GAGCTC 1 cut(s) 588
PspN4I GGNNCC 2 cut(s) 393, 649
PspPI GGNCC 3 cut(s) 47, 436, 507
PsuI RGATCY 1 cut(s) 410
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SacI GAGCTC 1 cut(s) 588
Sau3AI GATC 2 cut(s) 410, 535
Sau96I GGNCC 3 cut(s) 47, 436, 507
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 645
SduI GDGCHC 1 cut(s) 588
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 1 cut(s) 416
SinI GGWCC 2 cut(s) 47, 436
SmlI CTYRAG 2 cut(s) 70, 246
SmoI CTYRAG 2 cut(s) 70, 246
SsiI CCGC 8 cut(s) 12, 33, 54, 131, 143, 146, 573, 579
SspMI CTAG 2 cut(s) 21, 362
SstI GAGCTC 1 cut(s) 588
StyD4I CCNGG 1 cut(s) 643
TaaI ACNGT 4 cut(s) 51, 456, 531, 617
TaiI ACGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 483
TauI GCSGC 3 cut(s) 57, 134, 146
TscAI CASTG 3 cut(s) 600, 658, 675
TseI GCWGC 7 cut(s) 24, 27, 30, 93, 276, 367, 514
TspDTI ATGAA 1 cut(s) 324
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 3 cut(s) 600, 658, 675
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 595
VpaK11BI GGWCC 2 cut(s) 47, 436
XbaI TCTAGA 1 cut(s) 361
XspI CTAG 2 cut(s) 21, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.