Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008961

Physical Location & Seq

Genomic Coordinates Reverse (-)

106250 .. 111005

Gene Structure 4756 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G00240.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2784 bp

ATGGAGAATTATGGTTGGAATTACCCTTATTACCCTTACAGGCCTCCGCGGCCTAATCCTCCTTACCCAGCACCGCCTCATCACCATGGATCAATGTCTCATTCGGGTCCATTAGACCACCACCACCCTCCAATGAGTTACTATGCTTCTTTTGATTACCAACACCAACCTCCTCCTCCTTACCCTCCAGTGAGTTACTATGCTTCGTTTTCAAGTCATTCAGATTTATCATACTCAGGCAGGCTAGATTCGTCTGGTCATGGTTTTACATCTACCGCATCGCCGCATAGTCCCGGTATGCACATTGTGCCCTTTGGTAAAGCCTCCTTGAAGGTCCTCTTGTTGCATGGTAATTTGGATATTTGGGTTTCTTGTGCTAATAATCTACCAAACTTAGATTTGTTTCATAAGACATTGGGTGTTGTGTTTGGTGGCATGACCAATATGATCGAAGGCCAGTTGAGCAAGAAGATCACTAGTGATCCTTACGTGTCCATATCAGTCGCTGGTGCTGTCATAGGAAGAACATATGTCATTAGCAACAGCGAAAATCCTGTCTGGCAGCAGCATTTTTATGTCCCTGTAGCTCATCACGCTGCCGAGGTCCATTTTGTTGTTAAGGACAGTGATGCCGTCGGTTCACAGCTTATAGGAATTGTTACAATCCCTGTTGAACAAATTTACTCAGGTGCAAGAATTGAAGGGACTTACTCCATTCGAGACAGCAATGGAAAGCCTTGTAAACCAGGGGCAACTCTATCATTGTCGATTCAGTATACTTCAATGAATAAACTCAGTGTTTATCACAGCGGAGTTGGTGCAGGTCCCTATTACCAGGGGGTGCCTGGAACATATTTTCCTCTTAGGGAAGGTGGAAGTGTCACATTGTACCAAGATGCACATGTACCTGAAGGGATGCTCCCGGGAATAAAACTCGGAAATGGAATGTGTTACGAACATGGAAAGTGTTGGCATGATATGTTTCATGCCATTTGTCAAGCTAGACGTTTGATTTATATTACAGGCTGGTCAGTGTGGCATAATGTCAGGCTTGTTCGGGATAAAGAAGATCCTTCTTCAGAATGTAGACTAGGTGAGCTCCTAAGATCAAAGTCCCAAGAAGGTGTGAGAGTCCTGCTTTTGGTTTGGGACGACCCAACTTCACAAAACATCTTGGGATACATGACCGATGGGGTTATGGGAACCCATGACGAGGAGACACGCCGTTTTTTCAAGGACTCCTCAGTGCAAGTCTTGCTATGCCCTCGAAATGCTGGAAAGCGACATAGTTGGGTCAAACAGAGGGAAGTTGGAACCATCTATACACACCACCAAAAAAACTTAATAGTAGATGCCGATGCTGGGGGTAACAGAAGGAAAATCGTAGCTTTTGTGGGCGGGCTTGATCTATGTGATGGCCGATATGATACTCCTCAACATCCGTTGTTTAGGACACTGCAAACAGATCATAATGGTGACTATCACAACCCCACTTTCACGGGTAACGTGTCCGGTTGTCCAAGAGAACCTTGGCATGACTTGCACAGTAAGATTGATGGCCCAGCTGCGTATGATGTGCTGACAAATTTTGAGGAGAGGTGGCTGAAGGCTGCTAAGCCTCACAGAATCAATAAATTGAAGACGTCATATGATGATGCACTACTAAGGATCGACAGGATTCCAGATATATTAAGAGTTTTGGATGCTCCTACTGTTAGTGCAAACGACCCCGAGGCTTGGCATGTTCAGATTTTCCGGTCAATCGATTCAAACTCTGTGAAAGGTTTTCCAAAGGATCCAAAATATGCTACAAGCAAGAATTTGGTGTGCGGTAAGAATGTGCTCATTGACATGAGCATACACACCGCATATGTAAAGGCCATTCGTGCAGCTCAACACTTCATCTACATCGAGAACCAGTATTTCATTGGATCCTCCTACGACTGGAATGCTCACAAGGACATAGGTGCCAACAACCTGATTCCTATGGAAATTGCGTTGAAGATTGCTGATAAGATCAGAGCAAAGGAACGGTTTGCTGCATATATTGTCATTCCAATGTGGCCAGAAGGAGTTCCTACCGGCGCCGCTACACAAAGAATTCTATATTGGCAGCACAAGACGATGCAAATGATGTATGGAACTATCTACAATGCGTTAGTGGAGGCCGGGCTTGAAGATGAATACTCCCCACAAGACTATTTGAATTTCTTCTGTCTTGGAAACCGAGAAATGGTGAACGGAAATAATGAATCGGGTACAGGAAGTGCAAGCAACGAGAACACTCCTCAGGGATTATGTCGAAAGAGCAGAAGATTTATGATATATGTTCATTCAAAAGGGATGGTTGTTGATGATGAGTATGTGGTAATTGGATCTGCAAACATAAACCAAAGATCAATGGAGGGGACAAGAGATACAGAGATTGCAATGGGAGCTTACCAGCCTCAACATACATGGGCACGACGACAGTCAGGTCCTCGGGGTCAGATCTACGGATATAGAATGTCGCTTTGGGCAGAACACATGGCACTGCTAGATGATTGCTTTGTGGAGCCAGAGAGTCTAGGGTGTGTAAGGAAAGTGAGAACAGTGGCGGAGGAGAACTGGGAACAGTTTAGGAGTGAGGAAGTGTCGGAGATGAGAGGACATCTGATGAAATATCCGGTGGAAGTTGATCGGAAAGGCAAAGTCCGACCACTTCCAGGAAGTGAGGAGTTCCCTGATGTCGGAGGAAACGTTGTTGGATCCTTTCTAGCCATACAGGAGAATCTCACTATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005488 GO:0005543 GO:0005546 GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0008081 GO:0008150 GO:0008289 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0010035 GO:0010038 GO:0016298 GO:0016787 GO:0016788 GO:0030054 GO:0035091 GO:0042221 GO:0042578 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0045087 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542 GO:1901981 GO:1902936

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

927

Amino Acids

104.15

Weight (kDa)

6.76

Isoelectric Point (pI)

40.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	6 - 86	5.6e-14	C2 domain
PLDc	PF00614	289 - 322	7.5e-06	Phospholipase D Active site motif
PLDc	PF00614	623 - 648	3.6e-08	Phospholipase D Active site motif
PLDc_2	PF13091	623 - 659	5e-06	PLD-like domain
PLD_C	PF12357	695 - 765	5.4e-31	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42010 AT2G42010 AT4G00240 AT4G00240 AT4G00240
fragaria_vesca	FvH4_7g12760
malus_domestica	MD07G1124600.v1.1
prunus_persica	Prupe.2G152100_v2.0.a1 Prupe.2G152100_v2.0.a1
pyrus_communis	pycom07g11820
rosa_chinensis	RchiOBHm_Chr1g0313111 RchiOBHm_Chr1g0351791
rosa_laevigata	RLG00000028383
rosa_multiflora	Rmu_sc0001933.1_g000011
rosa_roxburghii	Rroxscaffold_4G00303540
rosa_rugosa	Rorug01G0221600
rosa_samantha	Rh1AG000800 Rh1AG235500 Rh1BG205900 Rh1CG005200 Rh1DG233000
rosa_wichuraiana	Rw1G020500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2475
AatII	GACGTC	1 cut(s)	1646
Acc36I	ACCTGC	1 cut(s)	812
AccB1I	GGYRCC	3 cut(s)	841, 1967, 2084
AccI	GTMKAC	2 cut(s)	776, 1087
AccII	CGCG	1 cut(s)	49
AclI	AACGTT	1 cut(s)	2739
AcoI	YGGCCR	2 cut(s)	1417, 2063
AcsI	RAATTY	5 cut(s)	678, 1585, 1819, 2100, 2206
AcuI	CTGAAG	3 cut(s)	930, 1062, 1625
AcyI	GRCGYC	2 cut(s)	1643, 2085
AdeI	CACNNNGTG	1 cut(s)	307
AfaI	GTAC	3 cut(s)	890, 906, 2260
AfiI	CCNNNNNNNGG	8 cut(s)	1141, 1213, 1362, 1737, 1944, 2233, 2705, 2729
AflIII	ACRYGT	3 cut(s)	489, 901, 1506
AhlI	ACTAGT	1 cut(s)	476
AjnI	CCWGG	4 cut(s)	745, 834, 844, 2704
AjuI	GAANNNNNNNTTGG	4 cut(s)	2092, 2124, 2497, 2529
AloI	GAACNNNNNNTCC	2 cut(s)	841, 873
AluBI	AGCT	8 cut(s)	587, 646, 1001, 1099, 1388, 1565, 1892, 2438
AluI	AGCT	8 cut(s)	587, 646, 1001, 1099, 1388, 1565, 1892, 2438
Alw21I	GWGCWC	2 cut(s)	1101, 1845
Alw26I	GTCTC	3 cut(s)	102, 714, 1211
AlwNI	CAGNNNCTG	1 cut(s)	506
Ama87I	CYCGRG	3 cut(s)	922, 1730, 2481
AoxI	GGCC	8 cut(s)	41, 50, 454, 1417, 1558, 1878, 2063, 2166
ApeKI	GCWGC	8 cut(s)	562, 565, 596, 1565, 1610, 1889, 2039, 2113
ApoI	RAATTY	5 cut(s)	678, 1585, 1819, 2100, 2206
ArsI	GACNNNNNNTTYG	2 cut(s)	540, 572
Asp700I	GAANNNNTTC	2 cut(s)	2073, 2210
AspLEI	GCGC	1 cut(s)	2087
AspS9I	GGNCC	6 cut(s)	107, 334, 604, 824, 1559, 2477
AsuC2I	CCSGG	4 cut(s)	294, 923, 924, 2170
AsuHPI	GGTGA	4 cut(s)	74, 1106, 1487, 2248
AvaI	CYCGRG	3 cut(s)	922, 1730, 2481
AvaII	GGWCC	5 cut(s)	107, 334, 604, 824, 2477
AxyI	CCTNAGG	1 cut(s)	2289
BaeGI	GKGCMC	2 cut(s)	312, 2464
BalI	TGGCCA	1 cut(s)	2065
BamHI	GGATCC	3 cut(s)	1795, 1931, 2747
BanI	GGYRCC	3 cut(s)	841, 1967, 2084
BanII	GRGCYC	1 cut(s)	1101
BbsI	GAAGAC	1 cut(s)	1646
Bbv12I	GWGCWC	2 cut(s)	1101, 1845
BbvI	GCAGC	8 cut(s)	574, 577, 583, 1552, 1597, 1901, 2026, 2125
BccI	CCATC	5 cut(s)	1184, 1325, 1409, 1550, 2338
BceAI	ACGGC	2 cut(s)	617, 1209
BcgI	CGANNNNNNTGC	2 cut(s)	1931, 1965
BciT130I	CCWGG	4 cut(s)	747, 836, 846, 2706
BciVI	GTATCC	1 cut(s)	1172
BcnI	CCSGG	4 cut(s)	294, 923, 924, 2170
BcoDI	GTCTC	3 cut(s)	102, 714, 1211
BcuI	ACTAGT	1 cut(s)	476
BfaI	CTAG	7 cut(s)	245, 477, 1002, 1091, 2537, 2567, 2756
BfmI	CTRYAG	1 cut(s)	582
BfoI	RGCGCY	1 cut(s)	2088
BfuAI	ACCTGC	1 cut(s)	812
BfuI	GTATCC	1 cut(s)	1172
BglI	GCCNNNNNGGC	1 cut(s)	49
BglII	AGATCT	1 cut(s)	2490
BlpI	GCTNAGC	1 cut(s)	1614
Bme1390I	CCNGG	8 cut(s)	294, 747, 836, 846, 923, 924, 2170, 2706
Bme18I	GGWCC	5 cut(s)	107, 334, 604, 824, 2477
BmeT110I	CYCGRG	3 cut(s)	922, 1730, 2481
BmgT120I	GGNCC	6 cut(s)	107, 334, 604, 824, 1559, 2477
BmrFI	CCNGG	8 cut(s)	294, 747, 836, 846, 923, 924, 2170, 2706
BmrI	ACTGGG	1 cut(s)	2617
BmsI	GCATC	9 cut(s)	287, 619, 886, 906, 1342, 1348, 1645, 1693, 2115
BmuI	ACTGGG	1 cut(s)	2617
BoxI	GACNNNNGTC	1 cut(s)	2470
BpiI	GAAGAC	1 cut(s)	1646
BpmI	CTGGAG	1 cut(s)	171
Bpu1102I	GCTNAGC	1 cut(s)	1614
BpuMI	CCSGG	4 cut(s)	294, 923, 924, 2170
Bsa29I	ATCGAT	1 cut(s)	1764
BsaAI	YACGTR	1 cut(s)	490
BsaHI	GRCGYC	2 cut(s)	1643, 2085
BsaJI	CCNNGG	9 cut(s)	47, 85, 600, 746, 835, 922, 1529, 1731, 2480
BsaWI	WCCGGW	3 cut(s)	1511, 1755, 2665
BsaXI	ACNNNNNCTCC	2 cut(s)	2724, 2754
Bsc4I	CCNNNNNNNGG	8 cut(s)	1141, 1213, 1362, 1737, 1944, 2233, 2705, 2729
Bse118I	RCCGGY	1 cut(s)	2081
Bse1I	ACTGG	5 cut(s)	188, 457, 1918, 1949, 2612
Bse21I	CCTNAGG	1 cut(s)	2289
Bse3DI	GCAATG	2 cut(s)	733, 2436
BseBI	CCWGG	4 cut(s)	747, 836, 846, 2706
BseCI	ATCGAT	1 cut(s)	1764
BseDI	CCNNGG	9 cut(s)	47, 85, 600, 746, 835, 922, 1529, 1731, 2480
BseGI	GGATG	4 cut(s)	921, 1438, 1708, 2349
BseLI	CCNNNNNNNGG	8 cut(s)	1141, 1213, 1362, 1737, 1944, 2233, 2705, 2729
BseMI	GCAATG	2 cut(s)	733, 2436
BseMII	CTCAG	5 cut(s)	249, 699, 808, 1257, 2303
BseNI	ACTGG	5 cut(s)	188, 457, 1918, 1949, 2612
BseRI	GAGGAG	9 cut(s)	162, 165, 1229, 1231, 1422, 1607, 2277, 2615, 2729
BseSI	GKGCMC	2 cut(s)	312, 2464
BseXI	GCAGC	8 cut(s)	574, 577, 583, 1552, 1597, 1901, 2026, 2125
BseYI	CCCAGC	3 cut(s)	67, 1361, 1561
BsgI	GTGCAG	2 cut(s)	840, 1908
Bsh1236I	CGCG	1 cut(s)	49
BshFI	GGCC	8 cut(s)	43, 52, 456, 1419, 1560, 1880, 2065, 2168
BshNI	GGYRCC	3 cut(s)	841, 1967, 2084
BshVI	ATCGAT	1 cut(s)	1764
BsiHKAI	GWGCWC	2 cut(s)	1101, 1845
BsiHKCI	CYCGRG	3 cut(s)	922, 1730, 2481
BsiSI	CCGG	7 cut(s)	294, 923, 1512, 1756, 2082, 2169, 2666
BslFI	GGGAC	7 cut(s)	276, 563, 718, 810, 1099, 1163, 2422
BslI	CCNNNNNNNGG	8 cut(s)	1141, 1213, 1362, 1737, 1944, 2233, 2705, 2729
BsmAI	GTCTC	3 cut(s)	102, 714, 1211
BsmFI	GGGAC	7 cut(s)	276, 563, 718, 810, 1099, 1163, 2422
BsmI	GAATGC	1 cut(s)	1954
BsnI	GGCC	8 cut(s)	43, 52, 456, 1419, 1560, 1880, 2065, 2168
BsoBI	CYCGRG	3 cut(s)	922, 1730, 2481
Bsp1286I	GDGCHC	4 cut(s)	312, 1101, 1845, 2464
Bsp1720I	GCTNAGC	1 cut(s)	1614
Bsp19I	CCATGG	1 cut(s)	85
BspANI	GGCC	8 cut(s)	43, 52, 456, 1419, 1560, 1880, 2065, 2168
BspCNI	CTCAG	5 cut(s)	248, 698, 807, 1256, 2302
BspDI	ATCGAT	1 cut(s)	1764
BspFNI	CGCG	1 cut(s)	49
BspMI	ACCTGC	1 cut(s)	812
BspT107I	GGYRCC	3 cut(s)	841, 1967, 2084
BsrDI	GCAATG	2 cut(s)	733, 2436
BsrFI	RCCGGY	1 cut(s)	2081
BsrI	ACTGG	5 cut(s)	188, 457, 1918, 1949, 2612
BssAI	RCCGGY	1 cut(s)	2081
BssECI	CCNNGG	9 cut(s)	47, 85, 600, 746, 835, 922, 1529, 1731, 2480
BssNAI	GTATAC	1 cut(s)	777
BssNI	GRCGYC	2 cut(s)	1643, 2085
BssT1I	CCWWGG	2 cut(s)	85, 1529
Bst1107I	GTATAC	1 cut(s)	777
Bst2UI	CCWGG	4 cut(s)	747, 836, 846, 2706
Bst4CI	ACNGT	7 cut(s)	626, 1547, 1714, 2034, 2472, 2593, 2616
BstACI	GRCGYC	2 cut(s)	1643, 2085
BstAPI	GCANNNNNTGC	3 cut(s)	307, 1255, 1540
BstBAI	YACGTR	1 cut(s)	490
BstC8I	GCNNGC	3 cut(s)	242, 1400, 2272
BstDSI	CCRYGG	2 cut(s)	47, 85
BstF5I	GGATG	4 cut(s)	921, 1438, 1708, 2349
BstFNI	CGCG	1 cut(s)	49
BstH2I	RGCGCY	1 cut(s)	2088
BstHHI	GCGC	1 cut(s)	2087
BstMAI	GTCTC	3 cut(s)	102, 714, 1211
BstMWI	GCNNNNNNNGC	8 cut(s)	49, 307, 462, 593, 1255, 1540, 1886, 2435
BstNI	CCWGG	4 cut(s)	747, 836, 846, 2706
BstNSI	RCATGY	2 cut(s)	905, 1745
BstPAI	GACNNNNGTC	1 cut(s)	2470
BstSCI	CCNGG	8 cut(s)	292, 745, 834, 844, 921, 922, 2168, 2704
BstSFI	CTRYAG	1 cut(s)	582
BstSLI	GKGCMC	2 cut(s)	312, 2464
BstUI	CGCG	1 cut(s)	49
BstV1I	GCAGC	8 cut(s)	574, 577, 583, 1552, 1597, 1901, 2026, 2125
BstV2I	GAAGAC	1 cut(s)	1646
BstX2I	RGATCY	6 cut(s)	1069, 1795, 1931, 2375, 2490, 2747
BstYI	RGATCY	6 cut(s)	1069, 1795, 1931, 2375, 2490, 2747
BstZ17I	GTATAC	1 cut(s)	777
Bsu15I	ATCGAT	1 cut(s)	1764
Bsu36I	CCTNAGG	1 cut(s)	2289
BsuI	GTATCC	1 cut(s)	1172
BsuRI	GGCC	8 cut(s)	43, 52, 456, 1419, 1560, 1880, 2065, 2168
BsuTUI	ATCGAT	1 cut(s)	1764
BtgI	CCRYGG	2 cut(s)	47, 85
BtgZI	GCGATG	1 cut(s)	264
BtsCI	GGATG	4 cut(s)	921, 1438, 1708, 2349
BtsI	GCAGTG	2 cut(s)	1454, 2531
BtsIMutI	CAGTG	8 cut(s)	195, 631, 802, 1038, 1251, 1454, 2531, 2598
BveI	ACCTGC	1 cut(s)	812
Cac8I	GCNNGC	3 cut(s)	242, 1400, 2272
CaiI	CAGNNNCTG	1 cut(s)	506
CfoI	GCGC	1 cut(s)	2087
Cfr10I	RCCGGY	1 cut(s)	2081
Cfr13I	GGNCC	6 cut(s)	107, 334, 604, 824, 1559, 2477
Cfr42I	CCGCGG	1 cut(s)	50
Cfr9I	CCCGGG	1 cut(s)	922
ClaI	ATCGAT	1 cut(s)	1764
Csp6I	GTAC	3 cut(s)	889, 905, 2259
CviQI	GTAC	3 cut(s)	889, 905, 2259
DinI	GGCGCC	1 cut(s)	2086
DraIII	CACNNNGTG	1 cut(s)	307
DrdI	GACNNNNNNGTC	1 cut(s)	2475
DseDI	GACNNNNNNGTC	1 cut(s)	2475
EaeI	YGGCCR	2 cut(s)	1417, 2063
EciI	GGCGGA	1 cut(s)	2612
Ecl136II	GAGCTC	1 cut(s)	1099
Eco130I	CCWWGG	2 cut(s)	85, 1529
Eco147I	AGGCCT	1 cut(s)	43
Eco24I	GRGCYC	1 cut(s)	1101
Eco47I	GGWCC	5 cut(s)	107, 334, 604, 824, 2477
Eco53kI	GAGCTC	1 cut(s)	1099
Eco57I	CTGAAG	3 cut(s)	930, 1062, 1625
Eco81I	CCTNAGG	1 cut(s)	2289
Eco88I	CYCGRG	3 cut(s)	922, 1730, 2481
EcoICRI	GAGCTC	1 cut(s)	1099
EcoO109I	RGGNCCY	3 cut(s)	334, 824, 2477
EcoRI	GAATTC	1 cut(s)	2100
EcoRII	CCWGG	4 cut(s)	745, 834, 844, 2704
EcoT14I	CCWWGG	2 cut(s)	85, 1529
EcoT38I	GRGCYC	1 cut(s)	1101
EgeI	GGCGCC	1 cut(s)	2086
EheI	GGCGCC	1 cut(s)	2086
ErhI	CCWWGG	2 cut(s)	85, 1529
FalI	AAGNNNNNCTT	4 cut(s)	323, 355, 1513, 1545
FaqI	GGGAC	7 cut(s)	276, 563, 718, 810, 1099, 1163, 2422
FauI	CCCGC	1 cut(s)	1391
FauNDI	CATATG	3 cut(s)	529, 1648, 1870
FblI	GTMKAC	2 cut(s)	776, 1087
FokI	GGATG	4 cut(s)	928, 1425, 1715, 2356
FriOI	GRGCYC	1 cut(s)	1101
FspBI	CTAG	7 cut(s)	245, 477, 1002, 1091, 2537, 2567, 2756
GlaI	GCGC	1 cut(s)	2086
GsaI	CCCAGC	3 cut(s)	71, 1365, 1565
GsuI	CTGGAG	1 cut(s)	171
HaeII	RGCGCY	1 cut(s)	2088
HaeIII	GGCC	8 cut(s)	43, 52, 456, 1419, 1560, 1880, 2065, 2168
HapII	CCGG	7 cut(s)	294, 923, 1512, 1756, 2082, 2169, 2666
HhaI	GCGC	1 cut(s)	2087
Hin1I	GRCGYC	2 cut(s)	1643, 2085
Hin6I	GCGC	1 cut(s)	2085
HinP1I	GCGC	1 cut(s)	2085
HpaII	CCGG	7 cut(s)	294, 923, 1512, 1756, 2082, 2169, 2666
HphI	GGTGA	4 cut(s)	74, 1106, 1487, 2248
Hpy166II	GTNNAC	5 cut(s)	641, 743, 777, 1088, 2239
Hpy188III	TCNNGA	4 cut(s)	719, 1058, 1682, 1912
Hpy8I	GTNNAC	5 cut(s)	641, 743, 777, 1088, 2239
Hpy99I	CGWCG	2 cut(s)	638, 2469
HpyCH4III	ACNGT	7 cut(s)	626, 1547, 1714, 2034, 2472, 2593, 2616
HpyCH4IV	ACGT	5 cut(s)	489, 1006, 1506, 1643, 2739
HpyF10VI	GCNNNNNNNGC	8 cut(s)	49, 307, 462, 593, 1255, 1540, 1886, 2435
HpySE526I	ACGT	5 cut(s)	489, 1006, 1506, 1643, 2739
Hsp92I	GRCGYC	2 cut(s)	1643, 2085
HspAI	GCGC	1 cut(s)	2085
KasI	GGCGCC	1 cut(s)	2084
KspI	CCGCGG	1 cut(s)	50
LmnI	GCTCC	5 cut(s)	924, 1104, 1711, 2435, 2554
Lsp1109I	GCAGC	8 cut(s)	574, 577, 583, 1552, 1597, 1901, 2026, 2125
LweI	GCATC	9 cut(s)	287, 619, 886, 906, 1342, 1348, 1645, 1693, 2115
MaeI	CTAG	7 cut(s)	245, 477, 1002, 1091, 2537, 2567, 2756
MaeII	ACGT	5 cut(s)	489, 1006, 1506, 1643, 2739
MaeIII	GTNAC	8 cut(s)	137, 194, 658, 880, 950, 1367, 1475, 1502
MboII	GAAGA	9 cut(s)	481, 534, 1068, 1079, 1651, 2014, 2189, 2203, 2325
MflI	RGATCY	6 cut(s)	1069, 1795, 1931, 2375, 2490, 2747
MhlI	GDGCHC	4 cut(s)	312, 1101, 1845, 2464
MlsI	TGGCCA	1 cut(s)	2065
MluNI	TGGCCA	1 cut(s)	2065
Mly113I	GGCGCC	1 cut(s)	2085
MlyI	GAGTC	3 cut(s)	1140, 1232, 2572
MmeI	TCCRAC	5 cut(s)	1291, 2616, 2710, 2719, 2725
Mox20I	TGGCCA	1 cut(s)	2065
MroXI	GAANNNNTTC	2 cut(s)	2073, 2210
MscI	TGGCCA	1 cut(s)	2065
MseI	TTAA	3 cut(s)	618, 1343, 1691
MslI	CAYNNNNRTG	5 cut(s)	84, 573, 1473, 1850, 2057
Msp20I	TGGCCA	1 cut(s)	2065
MspA1I	CMGCKG	3 cut(s)	49, 810, 1565
MspI	CCGG	7 cut(s)	294, 923, 1512, 1756, 2082, 2169, 2666
MspR9I	CCNGG	8 cut(s)	294, 747, 836, 846, 923, 924, 2170, 2706
Mva1269I	GAATGC	1 cut(s)	1954
MvaI	CCWGG	4 cut(s)	747, 836, 846, 2706
MvnI	CGCG	1 cut(s)	49
MwoI	GCNNNNNNNGC	8 cut(s)	49, 307, 462, 593, 1255, 1540, 1886, 2435
NarI	GGCGCC	1 cut(s)	2085
NciI	CCSGG	4 cut(s)	294, 923, 924, 2170
NcoI	CCATGG	1 cut(s)	85
NdeI	CATATG	3 cut(s)	529, 1648, 1870
NmeAIII	GCCGAG	1 cut(s)	625
NmuCI	GTSAC	2 cut(s)	880, 1475
NspI	RCATGY	2 cut(s)	905, 1745
PceI	AGGCCT	1 cut(s)	43
PciI	ACATGT	1 cut(s)	901
PcsI	WCGNNNNNNNCGW	1 cut(s)	2736
PctI	GAATGC	1 cut(s)	1954
PdmI	GAANNNNTTC	2 cut(s)	2073, 2210
PfeI	GAWTC	8 cut(s)	248, 769, 1626, 1678, 1766, 1981, 2252, 2770
PfoI	TCCNGGA	1 cut(s)	2704
PleI	GAGTC	3 cut(s)	1139, 1232, 2571
PluTI	GGCGCC	1 cut(s)	2088
PpsI	GAGTC	3 cut(s)	1139, 1232, 2571
Ppu21I	YACGTR	1 cut(s)	490
PpuMI	RGGWCCY	3 cut(s)	334, 824, 2477
PscI	ACATGT	1 cut(s)	901
PshAI	GACNNNNGTC	1 cut(s)	2470
Psp124BI	GAGCTC	1 cut(s)	1101
Psp1406I	AACGTT	1 cut(s)	2739
Psp5II	RGGWCCY	3 cut(s)	334, 824, 2477
Psp6I	CCWGG	4 cut(s)	745, 834, 844, 2704
PspFI	CCCAGC	3 cut(s)	67, 1361, 1561
PspGI	CCWGG	4 cut(s)	745, 834, 844, 2704
PspPI	GGNCC	6 cut(s)	107, 334, 604, 824, 1559, 2477
PspPPI	RGGWCCY	3 cut(s)	334, 824, 2477
PsrI	GAACNNNNNNTAC	2 cut(s)	1306, 1338
PstNI	CAGNNNCTG	1 cut(s)	506
PsuI	RGATCY	6 cut(s)	1069, 1795, 1931, 2375, 2490, 2747
PvuII	CAGCTG	1 cut(s)	1565
RsaI	GTAC	3 cut(s)	890, 906, 2260
RsaNI	GTAC	3 cut(s)	889, 905, 2259
RseI	CAYNNNNRTG	5 cut(s)	84, 573, 1473, 1850, 2057
SacI	GAGCTC	1 cut(s)	1101
SacII	CCGCGG	1 cut(s)	50
SaqAI	TTAA	3 cut(s)	618, 1343, 1691
Sau96I	GGNCC	6 cut(s)	107, 334, 604, 824, 1559, 2477
SchI	GAGTC	3 cut(s)	1140, 1232, 2572
ScrFI	CCNGG	8 cut(s)	294, 747, 836, 846, 923, 924, 2170, 2706
SduI	GDGCHC	4 cut(s)	312, 1101, 1845, 2464
SfaNI	GCATC	9 cut(s)	287, 619, 886, 906, 1342, 1348, 1645, 1693, 2115
SfcI	CTRYAG	1 cut(s)	582
SfiI	GGCCNNNNNGGCC	1 cut(s)	49
SfoI	GGCGCC	1 cut(s)	2086
Sfr303I	CCGCGG	1 cut(s)	50
SgrBI	CCGCGG	1 cut(s)	50
SinI	GGWCC	5 cut(s)	107, 334, 604, 824, 2477
SmaI	CCCGGG	1 cut(s)	924
SmiMI	CAYNNNNRTG	5 cut(s)	84, 573, 1473, 1850, 2057
SpeI	ACTAGT	1 cut(s)	476
SseBI	AGGCCT	1 cut(s)	43
SspDI	GGCGCC	1 cut(s)	2084
SspMI	CTAG	7 cut(s)	245, 477, 1002, 1091, 2537, 2567, 2756
SstI	GAGCTC	1 cut(s)	1101
StuI	AGGCCT	1 cut(s)	43
StyD4I	CCNGG	8 cut(s)	292, 745, 834, 844, 921, 922, 2168, 2704
StyI	CCWWGG	2 cut(s)	85, 1529
TaaI	ACNGT	7 cut(s)	626, 1547, 1714, 2034, 2472, 2593, 2616
TaiI	ACGT	5 cut(s)	492, 1009, 1509, 1646, 2742
TaqI	TCGA	8 cut(s)	450, 718, 767, 1267, 1671, 1764, 1911, 2302
TaqII	GACCGA	1 cut(s)	1202
TauI	GCSGC	3 cut(s)	52, 286, 2090
TfiI	GAWTC	8 cut(s)	248, 769, 1626, 1678, 1766, 1981, 2252, 2770
Tru1I	TTAA	3 cut(s)	618, 1343, 1691
Tru9I	TTAA	3 cut(s)	618, 1343, 1691
TscAI	CASTG	8 cut(s)	195, 631, 802, 1038, 1251, 1461, 2538, 2598
TseFI	GTSAC	2 cut(s)	880, 1475
TseI	GCWGC	8 cut(s)	562, 565, 596, 1565, 1610, 1889, 2039, 2113
Tsp45I	GTSAC	2 cut(s)	880, 1475
TspDTI	ATGAA	9 cut(s)	395, 800, 974, 1891, 1915, 2196, 2265, 2321, 2672
TspGWI	ACGGA	3 cut(s)	1431, 2256, 2511
TspMI	CCCGGG	1 cut(s)	922
TspRI	CASTG	8 cut(s)	195, 631, 802, 1038, 1251, 1461, 2538, 2598
VpaK11BI	GGWCC	5 cut(s)	107, 334, 604, 824, 2477
XapI	RAATTY	5 cut(s)	678, 1585, 1819, 2100, 2206
XceI	RCATGY	2 cut(s)	905, 1745
XcmI	CCANNNNNNNNNTGG	3 cut(s)	842, 1527, 1925
XmaI	CCCGGG	1 cut(s)	922
XmiI	GTMKAC	2 cut(s)	776, 1087
XmnI	GAANNNNTTC	2 cut(s)	2073, 2210
XspI	CTAG	7 cut(s)	245, 477, 1002, 1091, 2537, 2567, 2756
ZraI	GACGTC	1 cut(s)	1644

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.