Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0008961

Physical Location & Seq

Genomic Coordinates Reverse (-)

22043171 .. 22049386

Gene Structure 6216 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000028383

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3429 bp

ATGGATAACAATAGTTCTCCACCACCATACAATGGTTCACCACCATACTATCCACCATACCAGAATGCTTACCCACCATACCCACATCACCCTCCTTCTCAATACCCACCTCCTCCTTCAGATCAATACCCACCTGCTGCTCAATACCCTCCTTCACCAGATCAATACCCTCCTGCTTCTGCTCCTGCTCCATATCCATACCCTTATAGCTCATACCCTCCTTACCAACCTGCCCCATATCCATACCCTTCTTCAGCTTCAACTCCTTCATCTGCATACCCTCCCTCAGCTCCACCTCCTTCCTCTCCATACCCTCCTTCAGCTCCACCTCCTTCATCTGCATACCCTCCATCACCTTCACATTCTTCAACTACATACCCTCCATCACCTACATATCCACAATCACCATCCTCAGCCTCTTCCCATTCGGGCCCTTTCGACTACAATCAGCCCCCTTATCCCTATCCATACCCTCCGGCCCGGCCAAGTACAAATAGTGGTCCTCTGCCAACCCTTCAACACCAGGGCAGTGGTAGTTTTAAGTATGATTCTAACCATTATCAGTACCAGCAGTCAGGGATGTACCCGCCAGTAGAAAGCCCCCACCAATCCCCTCTGCGTGCCAGTAGGTTCTCTCTTCACCAGAGGTATGATAGTTGTCCTGTAGGAGTTGAGGCTTCTGGTTACCCCCCAGTGGATCAGCTTTTGAGTAATATGCATTTGTCTGAGAACCAACCAACTGATAATTCAGTGGCGCCGTCAGCACCTTCGAGTGCAAGGTGTGATAAGGTGGGGGAGTTTTATGGGTATCCTAATAGCTCCTTTTCGACTTGGGAGACAAGTAGTACTTATTCAGGGAATCAGACTGAACCGCCAAGTCCTGTTTTGACACCAACGACTTCAATCAATGGGTCTCAACATAGCCAGAGTTTGCAAATTGTTCCATTGCAGAATAAAGGGTCCTTGAAGGTTTTGCTCTTACATGGGAATCTGGATATTTGGGTGTATGAAGCGAAAAACCTGCCGAATATGGACATGTTCCACAAGACTTTGGGGGATATGTTTATGAAGTTTCCAGGGACTGGAAGCAACAAAGCTGATGGGCAGTCGAACCGTGGGATTACTAGTGATCCGTATGTTTCAATTTCAGTTGCAAATGCTGTAATTGGGAGGACATATGTGATAAGCAATTCTGAATTTCCTGTTTGGAGGCAACATTTCAATGTTCCAGTAGCGCATTATGCAGCTGAAGTGCACTTTGTTGTTAAAGATAGTGATCTTGTGGGATCCCAGCTTATTGGAGTTGTGGCAATTCCAGTAGAACATATATACTTGGGAGCAAGAGTTGAGGGAATTTACCCAATCCTGAATGCTAGTGGGAAGCCTTGTAAACCTGGAGCTGTCCTAAGGCTTTCAATTCAGTACACTCCAATTGAGAGATTGAGCATTTACCATGAAGGAGTTGGGGCAGGCCCTGATTACTATGGGGTTCCTGGCACCTATTTTCCTCTTAGGACAGGTGGAAAGGTAACTCTTTACCAAGATGCTCACGTCCCAGACGGTTGTCTTCCGAACTTGATTTTAGGTGGGGGAAGGCCATATGTGCATGGGAAGTGTTGGCATGACATTTTTGATGCAATACGTCGAGCACAGCGTTTGATTTACATTGCTGGGTGGTCGGTTTGGCACAATGTTAAACTGGTTCGGGATGGTGGTGCAGCATCAACTGTCACCATAGGTGATCTTCTGAGATCCAAGTCCCAGGAAGGAGTGAGAGTACTGCTGCTTGTGTGGGATGACCCCACTTCAAGGAGCATTTTGGGTTATAAAACTGATGGAATCATGCAAACCCATGATGAGGAAATTAGGCGGTTTTTTAAGCACTCTTCGGTGCAAGTGTTGCTTTGCCCCCGCACTGCTGGAAAACGACATAGCTGGGTTAAGCAAAGGGAAGTTGGGACGATCTATACGCATCATCAGAAAACCGTAATTGTGGATGCTGAGGCTGGCCATAATAGGAGGAAAATCCTAGCTTTTGTCGGAGGACTTGACTTATGTGATGGTCGATATGATACTCCAAACCATGAGCTATTTAAGACATTGAAAACAGTGCATAAAGATGACTATCACAACCCAACATTCACGGGTAGCACCACTGGTTGTCCAAGAGAGCCATGGCATGATTTGCACTCTAGGCTTGATGGTCCAGCAGCATATGATGTTCTGACCAACTTTGAGGAGCGCTGGTTAAAGGCTTCAAAACCACAAGGGATGAAGAAACTGAAAAGAACCTACAATGATTCTTTGCTCAAGTTGGAAAGGATTCCAGACATAATTGGAGCAGCCCATGCTGCTTCCACTAGTGACAACGATCCTGAAACTTGGCATGTTCAGATCTTTCGTTCAATTGATTCAAACTCTGTTAAAGGTTTCCCAAAGGATCCAAAAGAAGCAACAAGCAAGAATCTGGTATGCGGAAAGAATGTGCTGATCGACATGAGCATACATACAGCATATGTGAAGGCCATCCGTGCTGCCCAGCATTTCTTATATATAGAGAATCAATATTTTATTGGGTCTTCATACAATTGGAGTCAATACAAAGACTTAGGTGCTAATAATTTGATTCCCATGGAAATTGCCCTTAAGATTGCTGAGAAAATCAGAGCACACGAGAGATTCGCTGCGTATATTGTTATTCCAATGTGGCCAGAAGGTGTTCCGACTGGTGCAGCAACACAAAGAATTCTATTTTGGCAGCACAAGACAATGCAAATGATGTATGAGACAATTTACAAGGCGTTAGTGGAAATGGGGTTGGAGGGTGCATTCTGCCCCCAAGACTATTTAAACTTCTTTTGTCTTGGAAATCGTGAGGCTATAGATGGAAATGGTACATCTTTTTCTGGAAGTCCTGCTGCAGCAAACACTCCACAGGCATTTAGTCAAAAAAGCAGGCGATTTATGATTTATGTTCACTCGAAAGGTATGATAGTCGATGACGAGTATGTAATAGTGGGGTCTGCAAACATAAATCAGCGCTCTATGGAAGGGACAAGAGACACTGAAATAGCAATGGGGGCCTACCAACCTCATCATACATGGGCAAGAAATCATTCTAGTCCTTTCGGAGAGATATATGGATATAGAATGTCACTATGGGCAGAGCACACAGGAACCATTGAGGACTGTTTTAGAGAACCAGAGAGTCTTGAATGTGTAAGGAGAGTTAGAGCAATGGGTGAGATGAACTGGAGACAATTTGCTGCTGAGGATGCAACAGAGATGACGGGTCATCTATTGAAGTATCCAGTTGAAATTGATCGAAAAGGCAAAGTGACATCCCTTCCTGGATGTGAGAGTTTCCCCGACGTAGGAGGAAATATAACTGGTTCTTTCCTTGGCATTCAAGAAAATTTGACAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005488 GO:0005543 GO:0005546 GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0008081 GO:0008150 GO:0008289 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0010035 GO:0010038 GO:0016298 GO:0016787 GO:0016788 GO:0030054 GO:0035091 GO:0042221 GO:0042578 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0045087 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542 GO:1901981 GO:1902936

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

1143

Amino Acids

126.75

Weight (kDa)

6.84

Isoelectric Point (pI)

51.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	329 - 454	2.1e-13	C2 domain
PLDc	PF00614	656 - 689	5.8e-06	Phospholipase D Active site motif
PLDc_2	PF13091	989 - 1026	3.1e-06	PLD-like domain
PLDc	PF00614	990 - 1015	3.8e-08	Phospholipase D Active site motif
PLD_C	PF12357	1062 - 1132	9.5e-31	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42010 AT2G42010 AT4G00240 AT4G00240 AT4G00240
fragaria_vesca	FvH4_7g12760
malus_domestica	MD07G1124600.v1.1
prunus_persica	Prupe.2G152100_v2.0.a1 Prupe.2G152100_v2.0.a1
pyrus_communis	pycom07g11820
rosa_chinensis	RchiOBHm_Chr1g0313111 RchiOBHm_Chr1g0351791
rosa_laevigata	RLG00000028383
rosa_multiflora	Rmu_sc0001933.1_g000011
rosa_roxburghii	Rroxscaffold_4G00303540
rosa_rugosa	Rorug01G0221600
rosa_samantha	Rh1AG000800 Rh1AG235500 Rh1BG205900 Rh1CG005200 Rh1DG233000
rosa_wichuraiana	Rw1G020500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1827
AarI	CACCTGC	1 cut(s)	142
Acc36I	ACCTGC	3 cut(s)	142, 238, 1029
AccB1I	GGYRCC	2 cut(s)	754, 1496
AccB7I	CCANNNNNTGG	2 cut(s)	32, 1082
AciI	CCGC	5 cut(s)	587, 872, 1870, 1912, 2475
AclWI	GGATC	8 cut(s)	705, 1124, 1281, 1294, 1746, 2366, 2435, 2448
AcoI	YGGCCR	3 cut(s)	482, 2008, 2708
AcsI	RAATTY	4 cut(s)	1196, 1353, 2745, 3415
AcuI	CTGAAG	4 cut(s)	102, 237, 303, 1269
AcyI	GRCGYC	1 cut(s)	755
AfaI	GTAC	7 cut(s)	490, 566, 584, 847, 1424, 1779, 2896
AfeI	AGCGCT	2 cut(s)	2243, 3041
AfiI	CCNNNNNNNGG	6 cut(s)	32, 694, 1082, 1809, 1858, 3374
AflII	CTTAAG	1 cut(s)	2645
AflIII	ACRYGT	1 cut(s)	1035
AhlI	ACTAGT	2 cut(s)	1124, 2360
AjiI	CACGTC	1 cut(s)	1552
AjnI	CCWGG	6 cut(s)	522, 1075, 1393, 1492, 1761, 3349
AjuI	GAANNNNNNNTTGG	4 cut(s)	730, 762, 2737, 2769
Alw21I	GWGCWC	4 cut(s)	1257, 1651, 2671, 3171
Alw26I	GTCTC	5 cut(s)	830, 918, 2780, 3054, 3250
Alw44I	GTGCAC	1 cut(s)	1253
AlwI	GGATC	8 cut(s)	705, 1124, 1281, 1294, 1746, 2366, 2435, 2448
AlwNI	CAGNNNCTG	2 cut(s)	1082, 1475
Aor51HI	AGCGCT	2 cut(s)	2243, 3041
AoxI	GGCC	9 cut(s)	430, 477, 482, 1471, 1595, 2008, 2523, 2708, 3081
ApaI	GGGCCC	1 cut(s)	434
ApaLI	GTGCAC	1 cut(s)	1253
ApoI	RAATTY	4 cut(s)	1196, 1353, 2745, 3415
Asp700I	GAANNNNTTC	3 cut(s)	2322, 2718, 3115
AspLEI	GCGC	4 cut(s)	757, 1237, 2244, 3042
AspS9I	GGNCC	8 cut(s)	430, 431, 478, 500, 960, 1472, 2204, 3081
AsuC2I	CCSGG	1 cut(s)	481
AvaII	GGWCC	3 cut(s)	500, 960, 2204
AxyI	CCTNAGG	1 cut(s)	1406
BaeGI	GKGCMC	2 cut(s)	434, 1257
BalI	TGGCCA	2 cut(s)	2010, 2710
BamHI	GGATCC	2 cut(s)	1286, 2440
BanI	GGYRCC	2 cut(s)	754, 1496
BanII	GRGCYC	1 cut(s)	434
BauI	CACGAG	1 cut(s)	2672
BbsI	GAAGAC	2 cut(s)	1559, 2571
Bbv12I	GWGCWC	4 cut(s)	1257, 1651, 2671, 3171
BbvCI	CCTCAGC	4 cut(s)	286, 412, 2001, 3270
BceAI	ACGGC	1 cut(s)	742
BcgI	CGANNNNNNTGC	2 cut(s)	1003, 1037
BciT130I	CCWGG	6 cut(s)	524, 1077, 1395, 1494, 1763, 3351
BciVI	GTATCC	2 cut(s)	819, 3318
BcnI	CCSGG	1 cut(s)	481
BcoDI	GTCTC	5 cut(s)	830, 918, 2780, 3054, 3250
BcuI	ACTAGT	2 cut(s)	1124, 2360
BfaI	CTAG	6 cut(s)	1125, 1374, 2030, 2193, 2361, 3120
BfmI	CTRYAG	3 cut(s)	663, 2880, 2919
BfoI	RGCGCY	3 cut(s)	758, 2245, 3043
BfrI	CTTAAG	1 cut(s)	2645
BfuAI	ACCTGC	3 cut(s)	142, 238, 1029
BfuI	GTATCC	2 cut(s)	819, 3318
BglII	AGATCT	1 cut(s)	2394
BmcAI	AGTACT	2 cut(s)	847, 1779
Bme1390I	CCNGG	7 cut(s)	481, 524, 1077, 1395, 1494, 1763, 3351
Bme18I	GGWCC	3 cut(s)	500, 960, 2204
BmgBI	CACGTC	1 cut(s)	1552
BmgT120I	GGNCC	8 cut(s)	430, 431, 478, 500, 960, 1472, 2204, 3081
BmiI	GGNNCC	9 cut(s)	432, 756, 961, 1288, 1491, 1498, 2442, 3082, 3178
BmrFI	CCNGG	7 cut(s)	481, 524, 1077, 1395, 1494, 1763, 3351
BmrI	ACTGGG	1 cut(s)	686
BmsI	GCATC	6 cut(s)	1534, 1624, 1730, 1981, 1987, 3265
BmuI	ACTGGG	1 cut(s)	686
BoxI	GACNNNNGTC	1 cut(s)	1563
BpiI	GAAGAC	2 cut(s)	1559, 2571
BpmI	CTGGAG	2 cut(s)	1416, 3274
Bpu10I	CCTNAGC	4 cut(s)	286, 412, 2001, 3270
BpuEI	CTTGAG	1 cut(s)	2294
BpuMI	CCSGG	1 cut(s)	481
BsaBI	GATNNNNATC	2 cut(s)	1275, 2124
BsaHI	GRCGYC	1 cut(s)	755
BsaI	GGTCTC	1 cut(s)	918
BsaJI	CCNNGG	7 cut(s)	523, 1076, 1114, 1761, 2174, 2631, 3400
BsaXI	ACNNNNNCTCC	8 cut(s)	364, 394, 1329, 1359, 1761, 1791, 2231, 2261
Bsc4I	CCNNNNNNNGG	6 cut(s)	32, 694, 1082, 1809, 1858, 3374
Bse21I	CCTNAGG	1 cut(s)	1406
Bse3DI	GCAATG	4 cut(s)	944, 1665, 3081, 3243
Bse8I	GATNNNNATC	2 cut(s)	1275, 2124
BseBI	CCWGG	6 cut(s)	524, 1077, 1395, 1494, 1763, 3351
BseDI	CCNNGG	7 cut(s)	523, 1076, 1114, 1761, 2174, 2631, 3400
BseJI	GATNNNNATC	2 cut(s)	1275, 2124
BseLI	CCNNNNNNNGG	6 cut(s)	32, 694, 1082, 1809, 1858, 3374
BseMI	GCAATG	4 cut(s)	944, 1665, 3081, 3243
BseMII	CTCAG	7 cut(s)	300, 426, 717, 1739, 1992, 2646, 3261
BseRI	GAGGAG	2 cut(s)	102, 2252
BseSI	GKGCMC	2 cut(s)	434, 1257
BseYI	CCCAGC	4 cut(s)	1290, 1670, 1935, 2538
BsgI	GTGCAG	2 cut(s)	1737, 2751
BshFI	GGCC	9 cut(s)	432, 479, 484, 1473, 1597, 2010, 2525, 2710, 3083
BshNI	GGYRCC	2 cut(s)	754, 1496
BsiHKAI	GWGCWC	4 cut(s)	1257, 1651, 2671, 3171
BsiSI	CCGG	2 cut(s)	476, 481
BslFI	GGGAC	5 cut(s)	1093, 1538, 1744, 1972, 3067
BslI	CCNNNNNNNGG	6 cut(s)	32, 694, 1082, 1809, 1858, 3374
BsmAI	GTCTC	5 cut(s)	830, 918, 2780, 3054, 3250
BsmFI	GGGAC	5 cut(s)	1093, 1538, 1744, 1972, 3067
BsmI	GAATGC	4 cut(s)	70, 1375, 2828, 3405
BsnI	GGCC	9 cut(s)	432, 479, 484, 1473, 1597, 2010, 2525, 2710, 3083
Bso31I	GGTCTC	1 cut(s)	918
Bsp120I	GGGCCC	1 cut(s)	430
Bsp1286I	GDGCHC	5 cut(s)	434, 1257, 1651, 2671, 3171
Bsp19I	CCATGG	2 cut(s)	2174, 2631
BspACI	CCGC	5 cut(s)	587, 872, 1870, 1912, 2475
BspANI	GGCC	9 cut(s)	432, 479, 484, 1473, 1597, 2010, 2525, 2710, 3083
BspCNI	CTCAG	7 cut(s)	299, 425, 718, 1740, 1993, 2647, 3262
BspLI	GGNNCC	9 cut(s)	432, 756, 961, 1288, 1491, 1498, 2442, 3082, 3178
BspMAI	CTGCAG	1 cut(s)	2923
BspMI	ACCTGC	3 cut(s)	142, 238, 1029
BspPI	GGATC	8 cut(s)	705, 1124, 1281, 1294, 1746, 2366, 2435, 2448
BspT107I	GGYRCC	2 cut(s)	754, 1496
BspTI	CTTAAG	1 cut(s)	2645
BspTNI	GGTCTC	1 cut(s)	918
BsrDI	GCAATG	4 cut(s)	944, 1665, 3081, 3243
BssECI	CCNNGG	7 cut(s)	523, 1076, 1114, 1761, 2174, 2631, 3400
BssNI	GRCGYC	1 cut(s)	755
BssSI	CACGAG	1 cut(s)	2672
BssT1I	CCWWGG	3 cut(s)	2174, 2631, 3400
Bst2BI	CACGAG	1 cut(s)	2672
Bst2UI	CCWGG	6 cut(s)	524, 1077, 1395, 1494, 1763, 3351
Bst4CI	ACNGT	6 cut(s)	1115, 1562, 1729, 1987, 2110, 3191
Bst6I	CTCTTC	3 cut(s)	424, 642, 1891
BstACI	GRCGYC	1 cut(s)	755
BstAFI	CTTAAG	1 cut(s)	2645
BstAPI	GCANNNNNTGC	3 cut(s)	1900, 2185, 2348
BstC8I	GCNNGC	4 cut(s)	621, 1471, 2008, 2957
BstDSI	CCRYGG	3 cut(s)	1114, 2174, 2631
BstEII	GGTNACC	1 cut(s)	683
BstH2I	RGCGCY	3 cut(s)	758, 2245, 3043
BstHHI	GCGC	4 cut(s)	757, 1237, 2244, 3042
BstMAI	GTCTC	5 cut(s)	830, 918, 2780, 3054, 3250
BstNI	CCWGG	6 cut(s)	524, 1077, 1395, 1494, 1763, 3351
BstNSI	RCATGY	2 cut(s)	1039, 2390
BstPAI	GACNNNNGTC	1 cut(s)	1563
BstPI	GGTNACC	1 cut(s)	683
BstSCI	CCNGG	7 cut(s)	479, 522, 1075, 1393, 1492, 1761, 3349
BstSFI	CTRYAG	3 cut(s)	663, 2880, 2919
BstSLI	GKGCMC	2 cut(s)	434, 1257
BstV2I	GAAGAC	2 cut(s)	1559, 2571
BstX2I	RGATCY	4 cut(s)	1286, 1751, 2394, 2440
BstXI	CCANNNNNNTGG	2 cut(s)	530, 1298
BstYI	RGATCY	4 cut(s)	1286, 1751, 2394, 2440
Bsu36I	CCTNAGG	1 cut(s)	1406
BsuI	GTATCC	2 cut(s)	819, 3318
BsuRI	GGCC	9 cut(s)	432, 479, 484, 1473, 1597, 2010, 2525, 2710, 3083
BtgI	CCRYGG	3 cut(s)	1114, 2174, 2631
BtrI	CACGTC	1 cut(s)	1552
BtsI	GCAGTG	2 cut(s)	535, 1914
BtsIMutI	CAGTG	7 cut(s)	535, 699, 756, 1914, 2115, 2154, 3063
BveI	ACCTGC	3 cut(s)	142, 238, 1029
Cac8I	GCNNGC	4 cut(s)	621, 1471, 2008, 2957
CaiI	CAGNNNCTG	2 cut(s)	1082, 1475
CfoI	GCGC	4 cut(s)	757, 1237, 2244, 3042
Cfr13I	GGNCC	8 cut(s)	430, 431, 478, 500, 960, 1472, 2204, 3081
Csp6I	GTAC	7 cut(s)	489, 565, 583, 846, 1423, 1778, 2895
CviQI	GTAC	7 cut(s)	489, 565, 583, 846, 1423, 1778, 2895
DinI	GGCGCC	1 cut(s)	756
DraI	TTTAAA	1 cut(s)	2850
EaeI	YGGCCR	3 cut(s)	482, 2008, 2708
Eam1104I	CTCTTC	3 cut(s)	424, 642, 1891
EarI	CTCTTC	3 cut(s)	424, 642, 1891
Eco130I	CCWWGG	3 cut(s)	2174, 2631, 3400
Eco24I	GRGCYC	1 cut(s)	434
Eco31I	GGTCTC	1 cut(s)	918
Eco47I	GGWCC	3 cut(s)	500, 960, 2204
Eco47III	AGCGCT	2 cut(s)	2243, 3041
Eco57I	CTGAAG	4 cut(s)	102, 237, 303, 1269
Eco81I	CCTNAGG	1 cut(s)	1406
Eco91I	GGTNACC	1 cut(s)	683
EcoO109I	RGGNCCY	4 cut(s)	431, 960, 1472, 3081
EcoO65I	GGTNACC	1 cut(s)	683
EcoRI	GAATTC	1 cut(s)	2745
EcoRII	CCWGG	6 cut(s)	522, 1075, 1393, 1492, 1761, 3349
EcoT14I	CCWWGG	3 cut(s)	2174, 2631, 3400
EcoT22I	ATGCAT	1 cut(s)	720
EcoT38I	GRGCYC	1 cut(s)	434
EgeI	GGCGCC	1 cut(s)	756
EheI	GGCGCC	1 cut(s)	756
ErhI	CCWWGG	3 cut(s)	2174, 2631, 3400
FalI	AAGNNNNNCTT	4 cut(s)	832, 864, 1887, 1919
FaqI	GGGAC	5 cut(s)	1093, 1538, 1744, 1972, 3067
FauI	CCCGC	2 cut(s)	594, 1919
FauNDI	CATATG	4 cut(s)	1177, 1600, 2215, 2515
FriOI	GRGCYC	1 cut(s)	434
FspBI	CTAG	6 cut(s)	1125, 1374, 2030, 2193, 2361, 3120
GlaI	GCGC	4 cut(s)	756, 1236, 2243, 3041
GsaI	CCCAGC	4 cut(s)	1294, 1674, 1939, 2542
GsuI	CTGGAG	2 cut(s)	1416, 3274
HaeII	RGCGCY	3 cut(s)	758, 2245, 3043
HaeIII	GGCC	9 cut(s)	432, 479, 484, 1473, 1597, 2010, 2525, 2710, 3083
HapII	CCGG	2 cut(s)	476, 481
HhaI	GCGC	4 cut(s)	757, 1237, 2244, 3042
Hin1I	GRCGYC	1 cut(s)	755
Hin6I	GCGC	4 cut(s)	755, 1235, 2242, 3040
HinP1I	GCGC	4 cut(s)	755, 1235, 2242, 3040
HpaII	CCGG	2 cut(s)	476, 481
Hpy166II	GTNNAC	5 cut(s)	38, 1255, 1391, 1425, 2977
Hpy188III	TCNNGA	9 cut(s)	992, 1366, 1706, 2327, 2375, 2873, 2907, 3212, 3410
Hpy8I	GTNNAC	5 cut(s)	38, 1255, 1391, 1425, 2977
Hpy99I	CGWCG	2 cut(s)	1647, 3374
HpyCH4III	ACNGT	6 cut(s)	1115, 1562, 1729, 1987, 2110, 3191
HpyCH4IV	ACGT	3 cut(s)	1551, 1642, 3372
HpySE526I	ACGT	3 cut(s)	1551, 1642, 3372
Hsp92I	GRCGYC	1 cut(s)	755
HspAI	GCGC	4 cut(s)	755, 1235, 2242, 3040
KasI	GGCGCC	1 cut(s)	754
LweI	GCATC	6 cut(s)	1534, 1624, 1730, 1981, 1987, 3265
MaeI	CTAG	6 cut(s)	1125, 1374, 2030, 2193, 2361, 3120
MaeII	ACGT	3 cut(s)	1551, 1642, 3372
MaeIII	GTNAC	6 cut(s)	683, 1528, 1729, 2363, 3153, 3337
MboII	GAAGA	9 cut(s)	243, 357, 411, 629, 1559, 1736, 1878, 2287, 2571
MfeI	CAATTG	3 cut(s)	1431, 2406, 2587
MflI	RGATCY	4 cut(s)	1286, 1751, 2394, 2440
MhlI	GDGCHC	5 cut(s)	434, 1257, 1651, 2671, 3171
MlsI	TGGCCA	2 cut(s)	2010, 2710
MluNI	TGGCCA	2 cut(s)	2010, 2710
Mly113I	GGCGCC	1 cut(s)	755
MlyI	GAGTC	2 cut(s)	2602, 3217
MmeI	TCCRAC	4 cut(s)	2020, 2295, 2747, 2799
Mox20I	TGGCCA	2 cut(s)	2010, 2710
Mph1103I	ATGCAT	1 cut(s)	720
MroXI	GAANNNNTTC	3 cut(s)	2322, 2718, 3115
MscI	TGGCCA	2 cut(s)	2010, 2710
MslI	CAYNNNNRTG	2 cut(s)	1221, 2118
Msp20I	TGGCCA	2 cut(s)	2010, 2710
MspA1I	CMGCKG	1 cut(s)	1247
MspCI	CTTAAG	1 cut(s)	2645
MspI	CCGG	2 cut(s)	476, 481
MspR9I	CCNGG	7 cut(s)	481, 524, 1077, 1395, 1494, 1763, 3351
MunI	CAATTG	3 cut(s)	1431, 2406, 2587
Mva1269I	GAATGC	4 cut(s)	70, 1375, 2828, 3405
MvaI	CCWGG	6 cut(s)	524, 1077, 1395, 1494, 1763, 3351
NarI	GGCGCC	1 cut(s)	755
NciI	CCSGG	1 cut(s)	481
NcoI	CCATGG	2 cut(s)	2174, 2631
NdeI	CATATG	4 cut(s)	1177, 1600, 2215, 2515
NlaIV	GGNNCC	9 cut(s)	432, 756, 961, 1288, 1491, 1498, 2442, 3082, 3178
NmuCI	GTSAC	4 cut(s)	1729, 2363, 3153, 3337
NsiI	ATGCAT	1 cut(s)	720
NspI	RCATGY	2 cut(s)	1039, 2390
PaqCI	CACCTGC	1 cut(s)	142
PciI	ACATGT	1 cut(s)	1035
PcsI	WCGNNNNNNNCGW	1 cut(s)	1651
PctI	GAATGC	4 cut(s)	70, 1375, 2828, 3405
PdmI	GAANNNNTTC	3 cut(s)	2322, 2718, 3115
PflMI	CCANNNNNTGG	2 cut(s)	32, 1082
PfoI	TCCNGGA	1 cut(s)	3349
PleI	GAGTC	2 cut(s)	2601, 3216
PluTI	GGCGCC	1 cut(s)	758
PpsI	GAGTC	2 cut(s)	2601, 3216
PpuMI	RGGWCCY	1 cut(s)	960
PscI	ACATGT	1 cut(s)	1035
PshAI	GACNNNNGTC	1 cut(s)	1563
PsiI	TTATAA	1 cut(s)	1827
Psp5II	RGGWCCY	1 cut(s)	960
Psp6I	CCWGG	6 cut(s)	522, 1075, 1393, 1492, 1761, 3349
PspEI	GGTNACC	1 cut(s)	683
PspFI	CCCAGC	4 cut(s)	1290, 1670, 1935, 2538
PspGI	CCWGG	6 cut(s)	522, 1075, 1393, 1492, 1761, 3349
PspN4I	GGNNCC	9 cut(s)	432, 756, 961, 1288, 1491, 1498, 2442, 3082, 3178
PspOMI	GGGCCC	1 cut(s)	430
PspPI	GGNCC	8 cut(s)	430, 431, 478, 500, 960, 1472, 2204, 3081
PspPPI	RGGWCCY	1 cut(s)	960
PstI	CTGCAG	1 cut(s)	2923
PstNI	CAGNNNCTG	2 cut(s)	1082, 1475
PsuI	RGATCY	4 cut(s)	1286, 1751, 2394, 2440
PvuII	CAGCTG	1 cut(s)	1247
RsaI	GTAC	7 cut(s)	490, 566, 584, 847, 1424, 1779, 2896
RsaNI	GTAC	7 cut(s)	489, 565, 583, 846, 1423, 1778, 2895
RseI	CAYNNNNRTG	2 cut(s)	1221, 2118
Sau96I	GGNCC	8 cut(s)	430, 431, 478, 500, 960, 1472, 2204, 3081
ScaI	AGTACT	2 cut(s)	847, 1779
SchI	GAGTC	2 cut(s)	2602, 3217
ScrFI	CCNGG	7 cut(s)	481, 524, 1077, 1395, 1494, 1763, 3351
SduI	GDGCHC	5 cut(s)	434, 1257, 1651, 2671, 3171
SfaNI	GCATC	6 cut(s)	1534, 1624, 1730, 1981, 1987, 3265
SfcI	CTRYAG	3 cut(s)	663, 2880, 2919
SfoI	GGCGCC	1 cut(s)	756
SinI	GGWCC	3 cut(s)	500, 960, 2204
SmiMI	CAYNNNNRTG	2 cut(s)	1221, 2118
SmlI	CTYRAG	2 cut(s)	2309, 2645
SmoI	CTYRAG	2 cut(s)	2309, 2645
SpeI	ACTAGT	2 cut(s)	1124, 2360
SsiI	CCGC	5 cut(s)	587, 872, 1870, 1912, 2475
SspDI	GGCGCC	1 cut(s)	754
SspI	AATATT	1 cut(s)	2567
SspMI	CTAG	6 cut(s)	1125, 1374, 2030, 2193, 2361, 3120
StyD4I	CCNGG	7 cut(s)	479, 522, 1075, 1393, 1492, 1761, 3349
StyI	CCWWGG	3 cut(s)	2174, 2631, 3400
TaaI	ACNGT	6 cut(s)	1115, 1562, 1729, 1987, 2110, 3191
TaiI	ACGT	3 cut(s)	1554, 1645, 3375
TatI	WGTACW	4 cut(s)	488, 845, 1422, 1777
TscAI	CASTG	7 cut(s)	535, 699, 756, 1921, 2115, 2161, 3070
TseFI	GTSAC	4 cut(s)	1729, 2363, 3153, 3337
Tsp45I	GTSAC	4 cut(s)	1729, 2363, 3153, 3337
TspDTI	ATGAA	8 cut(s)	258, 324, 1023, 1082, 1470, 2288, 2571, 3263
TspGWI	ACGGA	2 cut(s)	1122, 2519
TspRI	CASTG	7 cut(s)	535, 699, 756, 1921, 2115, 2161, 3070
Van91I	CCANNNNNTGG	2 cut(s)	32, 1082
Vha464I	CTTAAG	1 cut(s)	2645
VneI	GTGCAC	1 cut(s)	1253
VpaK11BI	GGWCC	3 cut(s)	500, 960, 2204
XapI	RAATTY	4 cut(s)	1196, 1353, 2745, 3415
XceI	RCATGY	2 cut(s)	1039, 2390
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1460, 2172
XmnI	GAANNNNTTC	3 cut(s)	2322, 2718, 3115
XspI	CTAG	6 cut(s)	1125, 1374, 2030, 2193, 2361, 3120
ZrmI	AGTACT	2 cut(s)	847, 1779
Zsp2I	ATGCAT	1 cut(s)	720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.