Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0008961

Physical Location & Seq

Genomic Coordinates Forward (+)

31790453 .. 31792591

Gene Structure 2139 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0221600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGCAACTTCTTTCTAATATCTTAAACGGAAGAAAGGTGATAATACACCGTGTTAGTGAAGGAATCACTAAGAATATTGCAGATACCATAAACTCTAAAAGAGTTCTTCTTGTTCTTGATGATGTGGATCATTTGGACCAATTAGATGCAGTACTTAGGATGCATGGCCGGTTTTATCCAGGAAGTAAAATCATAATAACAACAAGGCATTTGAGGTTGTTAAAGGCTCAGCAAGTTACTAAGGTGCATAATGTTGAAACTTTGGCTTACAAGGAATCATTGGAACTCTTCAGTTGGCATGCTTTCGGGCAGGACCTTCCCATCGAAGGTTACATGAAACTTTCAGAGAAGGTAGTACACCAGAGTGGAGGACTTCCGTTAGCTCTTAAAGTTTTGGGCTCTTCTTTATTTGGGGAGAGTTTAACTGTATGGGAAAGTGCGTTACAGAAATTAGAAACTATTCCTAATAGTGAAATCATGAATAAACTCAAAATAAGCTACGAATCTTTACAAGATGATCATGACCGCAAGTTGTTCCTCCACATTGCTTGTTTCTTTATTGGAAATGACAGATATAACGTTGTGAGGTTACTAGATGAGTGCAATTTGTATACTACTATTGGCATTCAAAATCTCATTGATAGAGGTTTAGTAACCATTGATGGATATGATAAGGTGCAGATGCATAATGTTATCCGTGACATGGGAAGAGGAATTGTTCGCCAAGAATCAGAGGATCCTGAAAAACGCAGTAGGTTATGGCATTATAAGGATTCTATCAAAGTACTCACAGAAAAGAATGGTACTGAGACCATTGAAGGTCTTGTTCTGAACAGGCATATGCACCCTGCAAGCACTCCTTCAGTAAACTCAAATGAGACAATCTTGGAAACCAATGCATTTTCAAGGATGTGCAAACTGAGACTACTCCATCTTAGTAATGTACAAGTCAATGGATGTTATAAAGAACTTCCTACAAAATTGAGATGGTTGTGTTGGCATGCATTTCCTCTGGATTGTATACCCGCTGATTTTCCTATGGCGAACCTGGTTGTTCTTGAAATGCATAATAGCAGCTTGACAGTTGACACAAATCTGGAAAGGGACCAAATTCTTGAAACACTTATTATATCTGGTTGCTCAAATCTGAATATGTTTCCAATGGAGATGATGAAGAACATGGAATCTCTTAAGACTACGTTATCTTTTCCAAGGACTACGTATGTGTATGTCTTTGGCCAACAATACAGCCTAGTTGAGAATGAATACATGTACAAGTTGGAACCCATTGGAAGTGATGTCTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005488 GO:0005543 GO:0005546 GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0008081 GO:0008150 GO:0008289 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0010035 GO:0010038 GO:0016298 GO:0016787 GO:0016788 GO:0030054 GO:0035091 GO:0042221 GO:0042578 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0045087 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542 GO:1901981 GO:1902936

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

435

Amino Acids

49.93

Weight (kDa)

6.36

Isoelectric Point (pI)

46.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NB-ARC	PF00931	22 - 104	7.2e-10	NB-ARC domain
WHD_ROQ1	PF23282	174 - 244	2.8e-22	Disease resistance protein Roq1-like, winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42010 AT2G42010 AT4G00240 AT4G00240 AT4G00240
fragaria_vesca	FvH4_7g12760
malus_domestica	MD07G1124600.v1.1
prunus_persica	Prupe.2G152100_v2.0.a1 Prupe.2G152100_v2.0.a1
pyrus_communis	pycom07g11820
rosa_chinensis	RchiOBHm_Chr1g0313111 RchiOBHm_Chr1g0351791
rosa_laevigata	RLG00000028383
rosa_multiflora	Rmu_sc0001933.1_g000011
rosa_roxburghii	Rroxscaffold_4G00303540
rosa_rugosa	Rorug01G0221600
rosa_samantha	Rh1AG000800 Rh1AG235500 Rh1BG205900 Rh1CG005200 Rh1DG233000
rosa_wichuraiana	Rw1G020500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	768, 963
AccI	GTMKAC	2 cut(s)	611, 1021
AciI	CCGC	2 cut(s)	526, 1026
AclI	AACGTT	1 cut(s)	579
AclWI	GGATC	3 cut(s)	135, 731, 744
AcoI	YGGCCR	2 cut(s)	166, 1237
AcsI	RAATTY	1 cut(s)	1110
AcuI	CTGAAG	2 cut(s)	274, 846
AfaI	GTAC	6 cut(s)	153, 357, 786, 805, 945, 1274
AfiI	CCNNNNNNNGG	2 cut(s)	326, 703
AflII	CTTAAG	1 cut(s)	1190
AflIII	ACRYGT	1 cut(s)	1269
AgsI	TTSAA	6 cut(s)	257, 629, 818, 906, 1061, 1118
AjnI	CCWGG	2 cut(s)	178, 1047
AleI	CACNNNNGTG	1 cut(s)	363
AluBI	AGCT	3 cut(s)	383, 498, 1077
AluI	AGCT	3 cut(s)	383, 498, 1077
Alw26I	GTCTC	3 cut(s)	803, 872, 916
AlwI	GGATC	3 cut(s)	135, 731, 744
AoxI	GGCC	2 cut(s)	166, 1237
ApeKI	GCWGC	1 cut(s)	1074
ApoI	RAATTY	1 cut(s)	1110
Asp700I	GAANNNNTTC	1 cut(s)	459
AspS9I	GGNCC	3 cut(s)	136, 313, 1105
AsuHPI	GGTGA	1 cut(s)	49
AvaII	GGWCC	3 cut(s)	136, 313, 1105
BalI	TGGCCA	1 cut(s)	1239
BamHI	GGATCC	1 cut(s)	736
BanII	GRGCYC	1 cut(s)	401
BbvI	GCAGC	1 cut(s)	1086
BccI	CCATC	4 cut(s)	329, 656, 939, 981
BciT130I	CCWGG	2 cut(s)	180, 1049
BclI	TGATCA	1 cut(s)	517
BcoDI	GTCTC	3 cut(s)	803, 872, 916
BfaI	CTAG	2 cut(s)	593, 1253
BfrI	CTTAAG	1 cut(s)	1190
BisI	GCNGC	1 cut(s)	1075
BlpI	GCTNAGC	1 cut(s)	228
BlsI	GCNGC	1 cut(s)	1076
BmcAI	AGTACT	2 cut(s)	153, 786
Bme1390I	CCNGG	2 cut(s)	180, 1049
Bme18I	GGWCC	3 cut(s)	136, 313, 1105
BmgT120I	GGNCC	3 cut(s)	136, 313, 1105
BmiI	GGNNCC	3 cut(s)	738, 1106, 1284
BmrFI	CCNGG	2 cut(s)	180, 1049
BmsI	GCATC	3 cut(s)	136, 150, 672
Bpu1102I	GCTNAGC	1 cut(s)	228
BsaAI	YACGTR	1 cut(s)	1221
BsaBI	GATNNNNATC	1 cut(s)	126
BsaI	GGTCTC	1 cut(s)	803
BsaJI	CCNNGG	1 cut(s)	1211
Bsc4I	CCNNNNNNNGG	2 cut(s)	326, 703
Bse118I	RCCGGY	1 cut(s)	168
Bse3DI	GCAATG	1 cut(s)	543
Bse8I	GATNNNNATC	1 cut(s)	126
BseBI	CCWGG	2 cut(s)	180, 1049
BseDI	CCNNGG	1 cut(s)	1211
BseGI	GGATG	3 cut(s)	165, 915, 962
BseJI	GATNNNNATC	1 cut(s)	126
BseLI	CCNNNNNNNGG	2 cut(s)	326, 703
BseMI	GCAATG	1 cut(s)	543
BseMII	CTCAG	3 cut(s)	242, 798, 911
BseXI	GCAGC	1 cut(s)	1086
BsgI	GTGCAG	1 cut(s)	698
BshFI	GGCC	2 cut(s)	168, 1239
BsiSI	CCGG	1 cut(s)	169
BslFI	GGGAC	1 cut(s)	1118
BslI	CCNNNNNNNGG	2 cut(s)	326, 703
BsmAI	GTCTC	3 cut(s)	803, 872, 916
BsmFI	GGGAC	1 cut(s)	1118
BsmI	GAATGC	1 cut(s)	624
BsnI	GGCC	2 cut(s)	168, 1239
Bso31I	GGTCTC	1 cut(s)	803
Bsp1286I	GDGCHC	1 cut(s)	401
Bsp1407I	TGTACA	2 cut(s)	943, 1272
Bsp143I	GATC	3 cut(s)	127, 517, 736
Bsp1720I	GCTNAGC	1 cut(s)	228
BspACI	CCGC	2 cut(s)	526, 1026
BspANI	GGCC	2 cut(s)	168, 1239
BspCNI	CTCAG	3 cut(s)	241, 799, 912
BspHI	TCATGA	2 cut(s)	477, 520
BspLI	GGNNCC	3 cut(s)	738, 1106, 1284
BspPI	GGATC	3 cut(s)	135, 731, 744
BspQI	GCTCTTC	1 cut(s)	406
BspTI	CTTAAG	1 cut(s)	1190
BspTNI	GGTCTC	1 cut(s)	803
BsrDI	GCAATG	1 cut(s)	543
BsrFI	RCCGGY	1 cut(s)	168
BsrGI	TGTACA	2 cut(s)	943, 1272
BssAI	RCCGGY	1 cut(s)	168
BssECI	CCNNGG	1 cut(s)	1211
BssMI	GATC	3 cut(s)	127, 517, 736
BssNAI	GTATAC	2 cut(s)	612, 1022
BssT1I	CCWWGG	1 cut(s)	1211
Bst1107I	GTATAC	2 cut(s)	612, 1022
Bst2UI	CCWGG	2 cut(s)	180, 1049
Bst4CI	ACNGT	3 cut(s)	50, 427, 1084
Bst6I	CTCTTC	3 cut(s)	293, 406, 703
BstAFI	CTTAAG	1 cut(s)	1190
BstAUI	TGTACA	2 cut(s)	943, 1272
BstBAI	YACGTR	1 cut(s)	1221
BstC8I	GCNNGC	3 cut(s)	300, 853, 1002
BstDEI	CTNAG	7 cut(s)	69, 155, 228, 240, 807, 920, 935
BstF5I	GGATG	3 cut(s)	165, 915, 962
BstKTI	GATC	3 cut(s)	130, 520, 739
BstMAI	GTCTC	3 cut(s)	803, 872, 916
BstMBI	GATC	3 cut(s)	127, 517, 736
BstNI	CCWGG	2 cut(s)	180, 1049
BstNSI	RCATGY	3 cut(s)	302, 1004, 1273
BstSCI	CCNGG	2 cut(s)	178, 1047
BstSNI	TACGTA	1 cut(s)	1221
BstV1I	GCAGC	1 cut(s)	1086
BstX2I	RGATCY	1 cut(s)	736
BstYI	RGATCY	1 cut(s)	736
BstZ17I	GTATAC	2 cut(s)	612, 1022
BsuRI	GGCC	2 cut(s)	168, 1239
BtsCI	GGATG	3 cut(s)	165, 915, 962
Cac8I	GCNNGC	3 cut(s)	300, 853, 1002
CciI	TCATGA	2 cut(s)	477, 520
Cfr10I	RCCGGY	1 cut(s)	168
Cfr13I	GGNCC	3 cut(s)	136, 313, 1105
CsiI	ACCWGGT	1 cut(s)	1047
Csp6I	GTAC	6 cut(s)	152, 356, 785, 804, 944, 1273
CspCI	CAANNNNNGTGG	2 cut(s)	530, 565
CviAII	CATG	9 cut(s)	164, 299, 334, 478, 521, 703, 1001, 1180, 1270
CviJI	RGCY	9 cut(s)	168, 227, 266, 383, 399, 498, 1077, 1239, 1251
CviKI_1	RGCY	9 cut(s)	168, 227, 266, 383, 399, 498, 1077, 1239, 1251
CviQI	GTAC	6 cut(s)	152, 356, 785, 804, 944, 1273
DdeI	CTNAG	7 cut(s)	69, 155, 228, 240, 807, 920, 935
DpnI	GATC	3 cut(s)	129, 519, 738
DpnII	GATC	3 cut(s)	127, 517, 736
EaeI	YGGCCR	2 cut(s)	166, 1237
Eam1104I	CTCTTC	3 cut(s)	293, 406, 703
EarI	CTCTTC	3 cut(s)	293, 406, 703
Eco105I	TACGTA	1 cut(s)	1221
Eco130I	CCWWGG	1 cut(s)	1211
Eco24I	GRGCYC	1 cut(s)	401
Eco31I	GGTCTC	1 cut(s)	803
Eco47I	GGWCC	3 cut(s)	136, 313, 1105
Eco57I	CTGAAG	2 cut(s)	274, 846
EcoO109I	RGGNCCY	1 cut(s)	313
EcoRII	CCWGG	2 cut(s)	178, 1047
EcoT14I	CCWWGG	1 cut(s)	1211
EcoT22I	ATGCAT	5 cut(s)	165, 687, 901, 1006, 1068
EcoT38I	GRGCYC	1 cut(s)	401
ErhI	CCWWGG	1 cut(s)	1211
FaeI	CATG	9 cut(s)	167, 302, 337, 481, 524, 706, 1004, 1183, 1273
FalI	AAGNNNNNCTT	2 cut(s)	844, 876
FaqI	GGGAC	1 cut(s)	1118
FatI	CATG	9 cut(s)	163, 298, 333, 477, 520, 702, 1000, 1179, 1269
FauI	CCCGC	1 cut(s)	1033
FauNDI	CATATG	1 cut(s)	840
FbaI	TGATCA	1 cut(s)	517
FblI	GTMKAC	2 cut(s)	611, 1021
Fnu4HI	GCNGC	1 cut(s)	1075
FokI	GGATG	3 cut(s)	172, 922, 969
FriOI	GRGCYC	1 cut(s)	401
Fsp4HI	GCNGC	1 cut(s)	1075
FspBI	CTAG	2 cut(s)	593, 1253
GluI	GCNGC	1 cut(s)	1075
HaeIII	GGCC	2 cut(s)	168, 1239
HapII	CCGG	1 cut(s)	169
Hin1II	CATG	9 cut(s)	167, 302, 337, 481, 524, 706, 1004, 1183, 1273
HincII	GTYRAC	1 cut(s)	1087
HindII	GTYRAC	1 cut(s)	1087
HinfI	GANTC	6 cut(s)	63, 275, 503, 728, 773, 1184
HpaII	CCGG	1 cut(s)	169
HphI	GGTGA	1 cut(s)	49
Hpy166II	GTNNAC	5 cut(s)	358, 612, 868, 1022, 1087
Hpy188I	TCNGA	4 cut(s)	346, 733, 831, 1149
Hpy188III	TCNNGA	8 cut(s)	116, 478, 521, 740, 1013, 1058, 1097, 1115
Hpy8I	GTNNAC	5 cut(s)	358, 612, 868, 1022, 1087
HpyAV	CCTTC	6 cut(s)	53, 320, 326, 343, 812, 870
HpyCH4III	ACNGT	3 cut(s)	50, 427, 1084
HpyCH4IV	ACGT	3 cut(s)	579, 1199, 1220
HpyF3I	CTNAG	7 cut(s)	69, 155, 228, 240, 807, 920, 935
HpySE526I	ACGT	3 cut(s)	579, 1199, 1220
Hsp92II	CATG	9 cut(s)	167, 302, 337, 481, 524, 706, 1004, 1183, 1273
Ksp22I	TGATCA	1 cut(s)	517
Kzo9I	GATC	3 cut(s)	127, 517, 736
LguI	GCTCTTC	1 cut(s)	406
Lsp1109I	GCAGC	1 cut(s)	1086
LweI	GCATC	3 cut(s)	136, 150, 672
MabI	ACCWGGT	1 cut(s)	1047
MaeI	CTAG	2 cut(s)	593, 1253
MaeII	ACGT	3 cut(s)	579, 1199, 1220
MaeIII	GTNAC	6 cut(s)	235, 329, 441, 588, 652, 698
MalI	GATC	3 cut(s)	129, 519, 738
MboI	GATC	3 cut(s)	127, 517, 736
MboII	GAAGA	6 cut(s)	42, 98, 280, 393, 720, 1186
MflI	RGATCY	1 cut(s)	736
MhlI	GDGCHC	1 cut(s)	401
MlsI	TGGCCA	1 cut(s)	1239
MluCI	AATT	6 cut(s)	140, 449, 604, 714, 980, 1110
MluNI	TGGCCA	1 cut(s)	1239
MmeI	TCCRAC	1 cut(s)	1260
MnlI	CCTC	8 cut(s)	207, 362, 548, 579, 638, 704, 727, 1020
Mox20I	TGGCCA	1 cut(s)	1239
Mph1103I	ATGCAT	5 cut(s)	165, 687, 901, 1006, 1068
MroXI	GAANNNNTTC	1 cut(s)	459
MscI	TGGCCA	1 cut(s)	1239
MseI	TTAA	6 cut(s)	23, 221, 387, 422, 1191, 1306
MslI	CAYNNNNRTG	1 cut(s)	363
Msp20I	TGGCCA	1 cut(s)	1239
MspA1I	CMGCKG	1 cut(s)	1028
MspCI	CTTAAG	1 cut(s)	1190
MspI	CCGG	1 cut(s)	169
MspR9I	CCNGG	2 cut(s)	180, 1049
Mva1269I	GAATGC	1 cut(s)	624
MvaI	CCWGG	2 cut(s)	180, 1049
NdeI	CATATG	1 cut(s)	840
NdeII	GATC	3 cut(s)	127, 517, 736
NlaIII	CATG	9 cut(s)	167, 302, 337, 481, 524, 706, 1004, 1183, 1273
NlaIV	GGNNCC	3 cut(s)	738, 1106, 1284
NmuCI	GTSAC	1 cut(s)	698
NsiI	ATGCAT	5 cut(s)	165, 687, 901, 1006, 1068
NspI	RCATGY	3 cut(s)	302, 1004, 1273
OliI	CACNNNNGTG	1 cut(s)	363
PaeI	GCATGC	2 cut(s)	302, 1004
PagI	TCATGA	2 cut(s)	477, 520
PciI	ACATGT	1 cut(s)	1269
PciSI	GCTCTTC	1 cut(s)	406
PctI	GAATGC	1 cut(s)	624
PdmI	GAANNNNTTC	1 cut(s)	459
PfeI	GAWTC	6 cut(s)	63, 275, 503, 728, 773, 1184
PfoI	TCCNGGA	1 cut(s)	178
PkrI	GCNGC	1 cut(s)	1076
Ppu21I	YACGTR	1 cut(s)	1221
PpuMI	RGGWCCY	1 cut(s)	313
PscI	ACATGT	1 cut(s)	1269
PsiI	TTATAA	2 cut(s)	768, 963
Psp1406I	AACGTT	1 cut(s)	579
Psp5II	RGGWCCY	1 cut(s)	313
Psp6I	CCWGG	2 cut(s)	178, 1047
PspGI	CCWGG	2 cut(s)	178, 1047
PspN4I	GGNNCC	3 cut(s)	738, 1106, 1284
PspPI	GGNCC	3 cut(s)	136, 313, 1105
PspPPI	RGGWCCY	1 cut(s)	313
PsuI	RGATCY	1 cut(s)	736
RsaI	GTAC	6 cut(s)	153, 357, 786, 805, 945, 1274
RsaNI	GTAC	6 cut(s)	152, 356, 785, 804, 944, 1273
RseI	CAYNNNNRTG	1 cut(s)	363
SapI	GCTCTTC	1 cut(s)	406
SaqAI	TTAA	6 cut(s)	23, 221, 387, 422, 1191, 1306
SatI	GCNGC	1 cut(s)	1075
Sau3AI	GATC	3 cut(s)	127, 517, 736
Sau96I	GGNCC	3 cut(s)	136, 313, 1105
ScaI	AGTACT	2 cut(s)	153, 786
ScrFI	CCNGG	2 cut(s)	180, 1049
SduI	GDGCHC	1 cut(s)	401
SexAI	ACCWGGT	1 cut(s)	1047
SfaNI	GCATC	3 cut(s)	136, 150, 672
SinI	GGWCC	3 cut(s)	136, 313, 1105
SmiMI	CAYNNNNRTG	1 cut(s)	363
SmlI	CTYRAG	1 cut(s)	1190
SmoI	CTYRAG	1 cut(s)	1190
SnaBI	TACGTA	1 cut(s)	1221
SphI	GCATGC	2 cut(s)	302, 1004
Sse9I	AATT	6 cut(s)	140, 449, 604, 714, 980, 1110
SsiI	CCGC	2 cut(s)	526, 1026
SspI	AATATT	1 cut(s)	76
SspMI	CTAG	2 cut(s)	593, 1253
StyD4I	CCNGG	2 cut(s)	178, 1047
StyI	CCWWGG	1 cut(s)	1211
TaaI	ACNGT	3 cut(s)	50, 427, 1084
TaiI	ACGT	3 cut(s)	582, 1202, 1223
TaqI	TCGA	1 cut(s)	324
TasI	AATT	6 cut(s)	140, 449, 604, 714, 980, 1110
TatI	WGTACW	5 cut(s)	151, 355, 784, 943, 1272
TfiI	GAWTC	6 cut(s)	63, 275, 503, 728, 773, 1184
Tru1I	TTAA	6 cut(s)	23, 221, 387, 422, 1191, 1306
Tru9I	TTAA	6 cut(s)	23, 221, 387, 422, 1191, 1306
TseFI	GTSAC	1 cut(s)	698
TseI	GCWGC	1 cut(s)	1074
Tsp45I	GTSAC	1 cut(s)	698
TspDTI	ATGAA	4 cut(s)	350, 494, 1187, 1278
TspGWI	ACGGA	3 cut(s)	42, 366, 686
Vha464I	CTTAAG	1 cut(s)	1190
VpaK11BI	GGWCC	3 cut(s)	136, 313, 1105
XapI	RAATTY	1 cut(s)	1110
XceI	RCATGY	3 cut(s)	302, 1004, 1273
XmiI	GTMKAC	2 cut(s)	611, 1021
XmnI	GAANNNNTTC	1 cut(s)	459
XspI	CTAG	2 cut(s)	593, 1253
ZrmI	AGTACT	2 cut(s)	153, 786
Zsp2I	ATGCAT	5 cut(s)	165, 687, 901, 1006, 1068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.