Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0008961

Physical Location & Seq

Genomic Coordinates Forward (+)

44153834 .. 44160014

Gene Structure 6181 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G020500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3429 bp

ATGGATAACAATGGTTCTCCACCACCATATTATCCACCATACCAGAATGCTTACCCACCATACCCACATCACCCTCCTTCTCAATACCCACCTCCTCCTTCAGATCAATACCCTCCTTCTCAATACCCTCCTCCACCAGATCAATACCCTCCTGCTCCTGCTCCTGCTCCATATCCATACCCTTATAGCTCATACCCTCCTTACCAACATGCCCCATATCCATACCCTTCTTCAGCTTCAACTCCTTCATCTGCATACCCTCCCTCAGCTCCACCTCCTTCCTCTGCATACCCTCCTTCAGCTCCACCTCCCTCATCTGCATACCCTCCATCACCTTCACATTCTTCAACTACATACCCTCCATCACCTGCATCATATCCACAATCACCATCCTCAGCCTCTTCCCATTCGGGCCCTTTCGACTACAATCAGCCCCCTTATCCCTATCCATACCCTCCGGCCCGGCCAACTACAAATAGTGGTCCTTTGCCAACCCTTCAGCACCAGGGGAGTGGTAGTTTTAAGTATGATTCTAACCATTACCAGTACCAGCAGTCAGGGATGTACCCGCCAGTAGAAAGCCCCCACCAGTCCCCTCTGCGTGCCAGTAGGTTCTCTCTTCACCAGAGGTATGATAGTTGTCCTGTAGGAGTTGAGGCTTCTGGTTACCCCCCAGTGGATCAGCTTTTGAGTAATATGCATTTGTCTGAGAACCAGCCAACTGATAATTCAGTGGGGCCGTCAGCACCTTCGAGTGCAAGGTGTGATAAGGTGGGGGAGTTTTATGGGTATCCTAATAGCTCCTTTACTACTTGGGAGGCGAGTAGTACTTATTCAGGGAATCAGACTGAACCGCCAAGTCCTGTTTTGACCCCAACAACTTCAATCAATGGGTCTCAACATAGCCAGAGTTTGCAGATTGTTCCATTGCAGAATAAAGGGTCCTTGAAGGTTTTGCTCTTACATGGGAATCTGGATATTTGGGTGTATGAAGCGAAAAACCTGCCTAATATGGACATGTTCCACAAGACTTTGGGGGATATGTTTATGAAGTTTCCAGGGACTGGAAGCAACAAAGCTGATGGGCAGTCGAACCGTGGGATTACTAGTGATCCCTATGTTTCAATTTCAGTTGCAAATGCTGTAATTGGGAGGACATATGTGATAAGCAATTCTGAATTTCCTGTTTGGAGGCAACATTTCAATGTTCCAGTAGCGCATTATGCAGCTGAAGTGCACTTCGTTGTTAAAGATAGTGATCTTGTGGGATCCCAGCTTATTGGAGTCGTGGCAATTCCAGTAGAACATATATACTTGGGAGCAAGAGTTGAGGGAATTTACCCAATCCTGAATGCTAGTGGGAAGCCTTGTAAACCCGGAGCTGTCCTAAGGCTTTCAATTCAGTACACTCCAATTGAGAGATTGAGCATTTACCATGATGGAGTTGGGGCAGGCCCTGATTACTATGGGGTTCCTGGCACCTATTTTCCTCTTAGGACAGGTGGAAAGGTAACTCTTTACCAAGATGCTCACGTCCCAGACGGTTGTCTTCCGAACTTGATTTTAGGTGGGGGGAGGCCATATGTGCATGGGAAGTGTTGGCATGACATTTTTGATGCAATACGTCAAGCACGGCGTTTGATTTACATTGCTGGGTGGTCGGTTTGGCACAATGTTAAGCTGGTTCGGGATGGTGGTGCAGCACTAAATGTCACCATAGGTGATCTTCTGAGATCCAAGTCCCAGGAAGGAGTGAGAGTACTGCTGCTTGTGTGGGATGACCCCACTTCAAGGAGCATTTTGGGTTATAAAACTGATGGAATCATGCAAACCCATGATGAGGAAATTCGGCGGTTTTTTAAGCACTCTTCGGTGCAAGTGTTGCTTTGCCCCGCACTGCTGGAAAACGACATAGCTGGGTTAAGCAAAGGTAATGTAACTTCTTTTCATGAAGTTGGGACGATCTATACGCATCATCAGAAAACCGTAATTGTGGATGCTGATGCTGGCCATAATAGGAGGAAAATCCTAGCTTTTGTCGGAGGACTTGACTTATGTGATGGTCGATATGATACTCCAAACCATGAGCTATTTAAGACATTGAAAACAGTGCATAAAGATGACTATCACAACCCAACGTTCACGGGTAGCACCGCTGGTTGTCCAAGAGAGCCATGGCATGATTTGCACTCTAGGCTTGATGGTCCAGCAGCATATGATGTTCTGACCAACTTTGAGGAGCGCTGGTTAAAGGCTTCAAAACCACAAGGGATGAAGAAACTGAAAAAATCTTACAATGATTCTTTGCTCAAGTTGGAAAGGATTCCAGACATAATTGGAGCAGCCCATGCTGCTTCCACTAGTGACAACGATCCTGAAACTTGGCATGTTCAGATCTTTCGTTCAATTGATTCAAACTCTGTTAAAGGTTTCCCAAAGGATCCAAAAGAAGCAACAAGCAAGAATCTGGTATGCGGAAAGAATGTGCTGATCGACATGAGCATACAAACAGCATATGTGAAGGCCATCCGTGCTGCCCAGCATTTCATATATGTAGAGAATCAATATTTTATTGGGTCTTCATACAATTGGAATCAATACAAAGACTTAGGTGCTAATAATTTGATTCCCATGGAAATTGCCCTTAAGATTGCTGAGAAAATCAGAGCACACGAGAGATTCGCTGCGTATATTGTTATTCCAATGTGGCCAGAAGGTGTTCCGACTGGTGCAGCAACACAAAGAATTCTATTTTGGCAGCACAAGACGATGCAAATGATGTATGAGACAATTTACAAGGCGTTAGTGGAAATGGGGTTGGAGGGTGCATTCTGCCCCCAAGACTATTTAAACTTCTTTTGTCTTGGAAATCGTGAGGCTATAGATGGAAATGATACATCTGTTTCTGGAAGTCCTGCAGCAGCAAACACTCCACAGGCATTTAGTCAAAAAAGCAGGCGATTTATGATTTATGTTCACTCGAAAGGTATGATAGTCGATGACGAGTATGTAATAGTGGGGTCTGCAAACATAAATCAGCGCTCTATGGAAGGGACAAGAGACACTGAAATAGCAATGGGGGCCTACCAACCTCATCATACATGGGCAAGAAATCATTCTAGTCCTTTCGGAGAGATATATGGATATAGAATGTCACTATGGGCAGAGCACACAGGAACCATTGAGGACTGTTTTAGAGAACCAGAGAGTCTTGAATGTGTCAGGAGAGTTAGAGCAATGGGTGAGATGAATTGGAAACAATTTGCTGCTGAGCATGCAACAGAGATGACGGGTCATCTATTGAAGTATCCAGTTGAAATTGATCGAAAAGGCAAAGTGACATCCCTTCCTGGATGTGAGAGTTTCCCCGACGTAGGAGGAAATATAACTGGTTCTTTCCTTGGCATTCAAGAAAATTTGACAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005488 GO:0005543 GO:0005546 GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0008081 GO:0008150 GO:0008289 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0010035 GO:0010038 GO:0016298 GO:0016787 GO:0016788 GO:0030054 GO:0035091 GO:0042221 GO:0042578 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0045087 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542 GO:1901981 GO:1902936

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

1142

Amino Acids

126.45

Weight (kDa)

6.49

Isoelectric Point (pI)

49.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	323 - 448	2.1e-13	C2 domain
PLDc	PF00614	656 - 689	1e-05	Phospholipase D Active site motif
PLDc_2	PF13091	989 - 1026	2.9e-06	PLD-like domain
PLDc	PF00614	990 - 1015	3.8e-08	Phospholipase D Active site motif
PLD_C	PF12357	1062 - 1132	6.8e-31	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42010 AT2G42010 AT4G00240 AT4G00240 AT4G00240
fragaria_vesca	FvH4_7g12760
malus_domestica	MD07G1124600.v1.1
prunus_persica	Prupe.2G152100_v2.0.a1 Prupe.2G152100_v2.0.a1
pyrus_communis	pycom07g11820
rosa_chinensis	RchiOBHm_Chr1g0313111 RchiOBHm_Chr1g0351791
rosa_laevigata	RLG00000028383
rosa_multiflora	Rmu_sc0001933.1_g000011
rosa_roxburghii	Rroxscaffold_4G00303540
rosa_rugosa	Rorug01G0221600
rosa_samantha	Rh1AG000800 Rh1AG235500 Rh1BG205900 Rh1CG005200 Rh1DG233000
rosa_wichuraiana	Rw1G020500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1809
AarI	CACCTGC	1 cut(s)	376
Acc36I	ACCTGC	2 cut(s)	376, 1011
AccB1I	GGYRCC	1 cut(s)	1478
AccB7I	CCANNNNNTGG	1 cut(s)	1064
AciI	CCGC	6 cut(s)	569, 854, 1852, 1893, 2154, 2475
AclI	AACGTT	1 cut(s)	2138
AclWI	GGATC	8 cut(s)	687, 1106, 1263, 1276, 1728, 2366, 2435, 2448
AcoI	YGGCCR	3 cut(s)	464, 2008, 2708
AcsI	RAATTY	5 cut(s)	1178, 1335, 1845, 2745, 3415
AcuI	CTGAAG	5 cut(s)	84, 216, 282, 482, 1251
AfaI	GTAC	5 cut(s)	548, 566, 829, 1406, 1761
AfeI	AGCGCT	2 cut(s)	2243, 3041
AfiI	CCNNNNNNNGG	5 cut(s)	676, 1064, 1791, 1840, 3374
AflII	CTTAAG	1 cut(s)	2645
AflIII	ACRYGT	1 cut(s)	1017
AhlI	ACTAGT	2 cut(s)	1106, 2360
AjiI	CACGTC	1 cut(s)	1534
AjnI	CCWGG	5 cut(s)	504, 1057, 1474, 1743, 3349
AjuI	GAANNNNNNNTTGG	4 cut(s)	712, 744, 2737, 2769
Alw21I	GWGCWC	3 cut(s)	1239, 2671, 3171
Alw26I	GTCTC	3 cut(s)	900, 2780, 3054
Alw44I	GTGCAC	1 cut(s)	1235
AlwI	GGATC	8 cut(s)	687, 1106, 1263, 1276, 1728, 2366, 2435, 2448
AlwNI	CAGNNNCTG	2 cut(s)	1064, 1457
Aor51HI	AGCGCT	2 cut(s)	2243, 3041
ApaI	GGGCCC	1 cut(s)	416
ApaLI	GTGCAC	1 cut(s)	1235
ApoI	RAATTY	5 cut(s)	1178, 1335, 1845, 2745, 3415
Asp700I	GAANNNNTTC	3 cut(s)	2322, 2718, 3115
AspLEI	GCGC	3 cut(s)	1219, 2244, 3042
AspS9I	GGNCC	9 cut(s)	412, 413, 460, 482, 737, 942, 1454, 2204, 3081
AsuC2I	CCSGG	2 cut(s)	463, 1377
AsuHPI	GGTGA	8 cut(s)	62, 324, 357, 378, 614, 1705, 1733, 3254
AvaII	GGWCC	3 cut(s)	482, 942, 2204
AxyI	CCTNAGG	1 cut(s)	1388
BaeGI	GKGCMC	2 cut(s)	416, 1239
BaeI	ACNNNNGTAYC	2 cut(s)	2886, 2919
BalI	TGGCCA	2 cut(s)	2010, 2710
BamHI	GGATCC	2 cut(s)	1268, 2440
BanI	GGYRCC	1 cut(s)	1478
BanII	GRGCYC	1 cut(s)	416
BarI	GAAGNNNNNNTAC	2 cut(s)	1924, 1956
BauI	CACGAG	1 cut(s)	2672
BbsI	GAAGAC	2 cut(s)	1541, 2571
Bbv12I	GWGCWC	3 cut(s)	1239, 2671, 3171
BbvCI	CCTCAGC	2 cut(s)	265, 394
BceAI	ACGGC	2 cut(s)	724, 1649
BcgI	CGANNNNNNTGC	2 cut(s)	985, 1019
BciT130I	CCWGG	5 cut(s)	506, 1059, 1476, 1745, 3351
BciVI	GTATCC	2 cut(s)	801, 3318
BcnI	CCSGG	2 cut(s)	463, 1377
BcoDI	GTCTC	3 cut(s)	900, 2780, 3054
BcuI	ACTAGT	2 cut(s)	1106, 2360
BfaI	CTAG	6 cut(s)	1107, 1356, 2030, 2193, 2361, 3120
BfmI	CTRYAG	3 cut(s)	645, 2880, 2916
BfoI	RGCGCY	2 cut(s)	2245, 3043
BfrI	CTTAAG	1 cut(s)	2645
BfuAI	ACCTGC	2 cut(s)	376, 1011
BfuI	GTATCC	2 cut(s)	801, 3318
BglII	AGATCT	1 cut(s)	2394
BlpI	GCTNAGC	1 cut(s)	3270
BmcAI	AGTACT	2 cut(s)	829, 1761
Bme1390I	CCNGG	7 cut(s)	463, 506, 1059, 1377, 1476, 1745, 3351
Bme18I	GGWCC	3 cut(s)	482, 942, 2204
BmgBI	CACGTC	1 cut(s)	1534
BmgT120I	GGNCC	9 cut(s)	412, 413, 460, 482, 737, 942, 1454, 2204, 3081
BmiI	GGNNCC	9 cut(s)	414, 738, 943, 1270, 1473, 1480, 2442, 3082, 3178
BmrFI	CCNGG	7 cut(s)	463, 506, 1059, 1377, 1476, 1745, 3351
BmrI	ACTGGG	1 cut(s)	668
BmsI	GCATC	7 cut(s)	380, 1516, 1606, 1981, 1987, 1993, 2760
BmuI	ACTGGG	1 cut(s)	668
BoxI	GACNNNNGTC	1 cut(s)	1545
BpiI	GAAGAC	2 cut(s)	1541, 2571
Bpu10I	CCTNAGC	2 cut(s)	265, 394
Bpu1102I	GCTNAGC	1 cut(s)	3270
BpuEI	CTTGAG	1 cut(s)	2294
BpuMI	CCSGG	2 cut(s)	463, 1377
BsaBI	GATNNNNATC	2 cut(s)	1257, 2124
BsaI	GGTCTC	1 cut(s)	900
BsaJI	CCNNGG	7 cut(s)	505, 1058, 1096, 1743, 2174, 2631, 3400
Bsc4I	CCNNNNNNNGG	5 cut(s)	676, 1064, 1791, 1840, 3374
Bse21I	CCTNAGG	1 cut(s)	1388
Bse3DI	GCAATG	4 cut(s)	926, 1647, 3081, 3243
Bse8I	GATNNNNATC	2 cut(s)	1257, 2124
BseBI	CCWGG	5 cut(s)	506, 1059, 1476, 1745, 3351
BseDI	CCNNGG	7 cut(s)	505, 1058, 1096, 1743, 2174, 2631, 3400
BseGI	GGATG	9 cut(s)	389, 567, 1696, 1783, 2002, 2277, 2526, 3341, 3359
BseJI	GATNNNNATC	2 cut(s)	1257, 2124
BseLI	CCNNNNNNNGG	5 cut(s)	676, 1064, 1791, 1840, 3374
BseMI	GCAATG	4 cut(s)	926, 1647, 3081, 3243
BseMII	CTCAG	6 cut(s)	279, 408, 699, 1721, 2646, 3261
BseRI	GAGGAG	3 cut(s)	84, 120, 2252
BseSI	GKGCMC	2 cut(s)	416, 1239
BseYI	CCCAGC	4 cut(s)	1272, 1652, 1916, 2538
BsgI	GTGCAG	2 cut(s)	1719, 2751
BshNI	GGYRCC	1 cut(s)	1478
BsiHKAI	GWGCWC	3 cut(s)	1239, 2671, 3171
BsiSI	CCGG	3 cut(s)	458, 463, 1377
BslFI	GGGAC	6 cut(s)	577, 1075, 1520, 1726, 1972, 3067
BslI	CCNNNNNNNGG	5 cut(s)	676, 1064, 1791, 1840, 3374
BsmAI	GTCTC	3 cut(s)	900, 2780, 3054
BsmFI	GGGAC	6 cut(s)	577, 1075, 1520, 1726, 1972, 3067
BsmI	GAATGC	4 cut(s)	52, 1357, 2828, 3405
Bso31I	GGTCTC	1 cut(s)	900
Bsp120I	GGGCCC	1 cut(s)	412
Bsp1286I	GDGCHC	4 cut(s)	416, 1239, 2671, 3171
Bsp1720I	GCTNAGC	1 cut(s)	3270
Bsp19I	CCATGG	2 cut(s)	2174, 2631
BspACI	CCGC	6 cut(s)	569, 854, 1852, 1893, 2154, 2475
BspCNI	CTCAG	6 cut(s)	278, 407, 700, 1722, 2647, 3262
BspHI	TCATGA	1 cut(s)	1948
BspLI	GGNNCC	9 cut(s)	414, 738, 943, 1270, 1473, 1480, 2442, 3082, 3178
BspMAI	CTGCAG	1 cut(s)	2920
BspMI	ACCTGC	2 cut(s)	376, 1011
BspPI	GGATC	8 cut(s)	687, 1106, 1263, 1276, 1728, 2366, 2435, 2448
BspT107I	GGYRCC	1 cut(s)	1478
BspTI	CTTAAG	1 cut(s)	2645
BspTNI	GGTCTC	1 cut(s)	900
BsrDI	GCAATG	4 cut(s)	926, 1647, 3081, 3243
BssECI	CCNNGG	7 cut(s)	505, 1058, 1096, 1743, 2174, 2631, 3400
BssSI	CACGAG	1 cut(s)	2672
BssT1I	CCWWGG	3 cut(s)	2174, 2631, 3400
Bst2BI	CACGAG	1 cut(s)	2672
Bst2UI	CCWGG	5 cut(s)	506, 1059, 1476, 1745, 3351
Bst4CI	ACNGT	5 cut(s)	1097, 1544, 1987, 2110, 3191
Bst6I	CTCTTC	3 cut(s)	406, 624, 1873
BstAFI	CTTAAG	1 cut(s)	2645
BstAPI	GCANNNNNTGC	3 cut(s)	1882, 2185, 2348
BstC8I	GCNNGC	5 cut(s)	603, 1453, 2008, 2957, 3276
BstDEI	CTNAG	9 cut(s)	265, 394, 708, 1388, 1493, 1730, 2608, 2655, 3270
BstDSI	CCRYGG	3 cut(s)	1096, 2174, 2631
BstEII	GGTNACC	1 cut(s)	665
BstF5I	GGATG	9 cut(s)	389, 567, 1696, 1783, 2002, 2277, 2526, 3341, 3359
BstH2I	RGCGCY	2 cut(s)	2245, 3043
BstHHI	GCGC	3 cut(s)	1219, 2244, 3042
BstMAI	GTCTC	3 cut(s)	900, 2780, 3054
BstNI	CCWGG	5 cut(s)	506, 1059, 1476, 1745, 3351
BstNSI	RCATGY	4 cut(s)	212, 1021, 2390, 3278
BstPAI	GACNNNNGTC	1 cut(s)	1545
BstPI	GGTNACC	1 cut(s)	665
BstSCI	CCNGG	7 cut(s)	461, 504, 1057, 1375, 1474, 1743, 3349
BstSFI	CTRYAG	3 cut(s)	645, 2880, 2916
BstSLI	GKGCMC	2 cut(s)	416, 1239
BstV2I	GAAGAC	2 cut(s)	1541, 2571
BstX2I	RGATCY	4 cut(s)	1268, 1733, 2394, 2440
BstXI	CCANNNNNNTGG	2 cut(s)	512, 1280
BstYI	RGATCY	4 cut(s)	1268, 1733, 2394, 2440
Bsu36I	CCTNAGG	1 cut(s)	1388
BsuI	GTATCC	2 cut(s)	801, 3318
BtgI	CCRYGG	3 cut(s)	1096, 2174, 2631
BtrI	CACGTC	1 cut(s)	1534
BtsCI	GGATG	9 cut(s)	389, 567, 1696, 1783, 2002, 2277, 2526, 3341, 3359
BtsI	GCAGTG	1 cut(s)	1895
BtsIMutI	CAGTG	5 cut(s)	681, 738, 1895, 2115, 3063
BveI	ACCTGC	2 cut(s)	376, 1011
Cac8I	GCNNGC	5 cut(s)	603, 1453, 2008, 2957, 3276
CaiI	CAGNNNCTG	2 cut(s)	1064, 1457
CciI	TCATGA	1 cut(s)	1948
CfoI	GCGC	3 cut(s)	1219, 2244, 3042
Cfr13I	GGNCC	9 cut(s)	412, 413, 460, 482, 737, 942, 1454, 2204, 3081
Csp6I	GTAC	5 cut(s)	547, 565, 828, 1405, 1760
CviQI	GTAC	5 cut(s)	547, 565, 828, 1405, 1760
DdeI	CTNAG	9 cut(s)	265, 394, 708, 1388, 1493, 1730, 2608, 2655, 3270
DraI	TTTAAA	1 cut(s)	2850
EaeI	YGGCCR	3 cut(s)	464, 2008, 2708
Eam1104I	CTCTTC	3 cut(s)	406, 624, 1873
EarI	CTCTTC	3 cut(s)	406, 624, 1873
Eco130I	CCWWGG	3 cut(s)	2174, 2631, 3400
Eco24I	GRGCYC	1 cut(s)	416
Eco31I	GGTCTC	1 cut(s)	900
Eco47I	GGWCC	3 cut(s)	482, 942, 2204
Eco47III	AGCGCT	2 cut(s)	2243, 3041
Eco57I	CTGAAG	5 cut(s)	84, 216, 282, 482, 1251
Eco81I	CCTNAGG	1 cut(s)	1388
Eco91I	GGTNACC	1 cut(s)	665
EcoO109I	RGGNCCY	4 cut(s)	413, 942, 1454, 3081
EcoO65I	GGTNACC	1 cut(s)	665
EcoRI	GAATTC	1 cut(s)	2745
EcoRII	CCWGG	5 cut(s)	504, 1057, 1474, 1743, 3349
EcoT14I	CCWWGG	3 cut(s)	2174, 2631, 3400
EcoT22I	ATGCAT	1 cut(s)	702
EcoT38I	GRGCYC	1 cut(s)	416
ErhI	CCWWGG	3 cut(s)	2174, 2631, 3400
FalI	AAGNNNNNCTT	2 cut(s)	1869, 1901
FaqI	GGGAC	6 cut(s)	577, 1075, 1520, 1726, 1972, 3067
FauI	CCCGC	2 cut(s)	576, 1900
FauNDI	CATATG	4 cut(s)	1159, 1582, 2215, 2515
FokI	GGATG	9 cut(s)	376, 574, 1703, 1790, 2009, 2284, 2513, 3328, 3366
FriOI	GRGCYC	1 cut(s)	416
FspBI	CTAG	6 cut(s)	1107, 1356, 2030, 2193, 2361, 3120
GlaI	GCGC	3 cut(s)	1218, 2243, 3041
GsaI	CCCAGC	4 cut(s)	1276, 1656, 1920, 2542
HaeII	RGCGCY	2 cut(s)	2245, 3043
HapII	CCGG	3 cut(s)	458, 463, 1377
HhaI	GCGC	3 cut(s)	1219, 2244, 3042
Hin6I	GCGC	3 cut(s)	1217, 2242, 3040
HinP1I	GCGC	3 cut(s)	1217, 2242, 3040
HpaII	CCGG	3 cut(s)	458, 463, 1377
HphI	GGTGA	8 cut(s)	62, 324, 357, 378, 614, 1705, 1733, 3254
Hpy166II	GTNNAC	5 cut(s)	1237, 1373, 1407, 2142, 2977
Hpy8I	GTNNAC	5 cut(s)	1237, 1373, 1407, 2142, 2977
Hpy99I	CGWCG	1 cut(s)	3374
HpyCH4III	ACNGT	5 cut(s)	1097, 1544, 1987, 2110, 3191
HpyCH4IV	ACGT	4 cut(s)	1533, 1624, 2138, 3372
HpyF3I	CTNAG	9 cut(s)	265, 394, 708, 1388, 1493, 1730, 2608, 2655, 3270
HpySE526I	ACGT	4 cut(s)	1533, 1624, 2138, 3372
HspAI	GCGC	3 cut(s)	1217, 2242, 3040
LweI	GCATC	7 cut(s)	380, 1516, 1606, 1981, 1987, 1993, 2760
MaeI	CTAG	6 cut(s)	1107, 1356, 2030, 2193, 2361, 3120
MaeII	ACGT	4 cut(s)	1533, 1624, 2138, 3372
MaeIII	GTNAC	7 cut(s)	665, 1510, 1711, 1936, 2363, 3153, 3337
MboII	GAAGA	9 cut(s)	222, 336, 393, 611, 1541, 1718, 1860, 2287, 2571
MfeI	CAATTG	3 cut(s)	1413, 2406, 2587
MflI	RGATCY	4 cut(s)	1268, 1733, 2394, 2440
MhlI	GDGCHC	4 cut(s)	416, 1239, 2671, 3171
MlsI	TGGCCA	2 cut(s)	2010, 2710
MluNI	TGGCCA	2 cut(s)	2010, 2710
MlyI	GAGTC	2 cut(s)	1293, 3217
MmeI	TCCRAC	4 cut(s)	2020, 2295, 2747, 2799
Mox20I	TGGCCA	2 cut(s)	2010, 2710
Mph1103I	ATGCAT	1 cut(s)	702
MroXI	GAANNNNTTC	3 cut(s)	2322, 2718, 3115
MscI	TGGCCA	2 cut(s)	2010, 2710
MslI	CAYNNNNRTG	2 cut(s)	1203, 2118
Msp20I	TGGCCA	2 cut(s)	2010, 2710
MspA1I	CMGCKG	2 cut(s)	1229, 2156
MspCI	CTTAAG	1 cut(s)	2645
MspI	CCGG	3 cut(s)	458, 463, 1377
MspR9I	CCNGG	7 cut(s)	463, 506, 1059, 1377, 1476, 1745, 3351
MunI	CAATTG	3 cut(s)	1413, 2406, 2587
Mva1269I	GAATGC	4 cut(s)	52, 1357, 2828, 3405
MvaI	CCWGG	5 cut(s)	506, 1059, 1476, 1745, 3351
NciI	CCSGG	2 cut(s)	463, 1377
NcoI	CCATGG	2 cut(s)	2174, 2631
NdeI	CATATG	4 cut(s)	1159, 1582, 2215, 2515
NlaIV	GGNNCC	9 cut(s)	414, 738, 943, 1270, 1473, 1480, 2442, 3082, 3178
NmuCI	GTSAC	4 cut(s)	1711, 2363, 3153, 3337
NsiI	ATGCAT	1 cut(s)	702
NspI	RCATGY	4 cut(s)	212, 1021, 2390, 3278
PaeI	GCATGC	1 cut(s)	3278
PagI	TCATGA	1 cut(s)	1948
PaqCI	CACCTGC	1 cut(s)	376
PciI	ACATGT	1 cut(s)	1017
PctI	GAATGC	4 cut(s)	52, 1357, 2828, 3405
PdmI	GAANNNNTTC	3 cut(s)	2322, 2718, 3115
PflMI	CCANNNNNTGG	1 cut(s)	1064
PfoI	TCCNGGA	1 cut(s)	3349
PleI	GAGTC	2 cut(s)	1292, 3216
PpsI	GAGTC	2 cut(s)	1292, 3216
PpuMI	RGGWCCY	1 cut(s)	942
PscI	ACATGT	1 cut(s)	1017
PshAI	GACNNNNGTC	1 cut(s)	1545
PsiI	TTATAA	1 cut(s)	1809
Psp1406I	AACGTT	1 cut(s)	2138
Psp5II	RGGWCCY	1 cut(s)	942
Psp6I	CCWGG	5 cut(s)	504, 1057, 1474, 1743, 3349
PspEI	GGTNACC	1 cut(s)	665
PspFI	CCCAGC	4 cut(s)	1272, 1652, 1916, 2538
PspGI	CCWGG	5 cut(s)	504, 1057, 1474, 1743, 3349
PspN4I	GGNNCC	9 cut(s)	414, 738, 943, 1270, 1473, 1480, 2442, 3082, 3178
PspOMI	GGGCCC	1 cut(s)	412
PspPI	GGNCC	9 cut(s)	412, 413, 460, 482, 737, 942, 1454, 2204, 3081
PspPPI	RGGWCCY	1 cut(s)	942
PstI	CTGCAG	1 cut(s)	2920
PstNI	CAGNNNCTG	2 cut(s)	1064, 1457
PsuI	RGATCY	4 cut(s)	1268, 1733, 2394, 2440
PvuII	CAGCTG	1 cut(s)	1229
RsaI	GTAC	5 cut(s)	548, 566, 829, 1406, 1761
RsaNI	GTAC	5 cut(s)	547, 565, 828, 1405, 1760
RseI	CAYNNNNRTG	2 cut(s)	1203, 2118
Sau96I	GGNCC	9 cut(s)	412, 413, 460, 482, 737, 942, 1454, 2204, 3081
ScaI	AGTACT	2 cut(s)	829, 1761
SchI	GAGTC	2 cut(s)	1293, 3217
ScrFI	CCNGG	7 cut(s)	463, 506, 1059, 1377, 1476, 1745, 3351
SduI	GDGCHC	4 cut(s)	416, 1239, 2671, 3171
SfaNI	GCATC	7 cut(s)	380, 1516, 1606, 1981, 1987, 1993, 2760
SfcI	CTRYAG	3 cut(s)	645, 2880, 2916
SinI	GGWCC	3 cut(s)	482, 942, 2204
SmiMI	CAYNNNNRTG	2 cut(s)	1203, 2118
SmlI	CTYRAG	2 cut(s)	2309, 2645
SmoI	CTYRAG	2 cut(s)	2309, 2645
SpeI	ACTAGT	2 cut(s)	1106, 2360
SphI	GCATGC	1 cut(s)	3278
SsiI	CCGC	6 cut(s)	569, 854, 1852, 1893, 2154, 2475
SspI	AATATT	1 cut(s)	2567
SspMI	CTAG	6 cut(s)	1107, 1356, 2030, 2193, 2361, 3120
StyD4I	CCNGG	7 cut(s)	461, 504, 1057, 1375, 1474, 1743, 3349
StyI	CCWWGG	3 cut(s)	2174, 2631, 3400
TaaI	ACNGT	5 cut(s)	1097, 1544, 1987, 2110, 3191
TaiI	ACGT	4 cut(s)	1536, 1627, 2141, 3375
TaqI	TCGA	8 cut(s)	420, 752, 1091, 2065, 2493, 2981, 2997, 3325
TatI	WGTACW	3 cut(s)	827, 1404, 1759
TscAI	CASTG	5 cut(s)	681, 738, 1902, 2115, 3070
TseFI	GTSAC	4 cut(s)	1711, 2363, 3153, 3337
Tsp45I	GTSAC	4 cut(s)	1711, 2363, 3153, 3337
TspDTI	ATGAA	9 cut(s)	237, 1005, 1064, 1937, 1965, 2288, 2536, 2571, 3263
TspGWI	ACGGA	1 cut(s)	2519
TspRI	CASTG	5 cut(s)	681, 738, 1902, 2115, 3070
Van91I	CCANNNNNTGG	1 cut(s)	1064
Vha464I	CTTAAG	1 cut(s)	2645
VneI	GTGCAC	1 cut(s)	1235
VpaK11BI	GGWCC	3 cut(s)	482, 942, 2204
XapI	RAATTY	5 cut(s)	1178, 1335, 1845, 2745, 3415
XceI	RCATGY	4 cut(s)	212, 1021, 2390, 3278
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1442, 2172
XmnI	GAANNNNTTC	3 cut(s)	2322, 2718, 3115
XspI	CTAG	6 cut(s)	1107, 1356, 2030, 2193, 2361, 3120
ZrmI	AGTACT	2 cut(s)	829, 1761
Zsp2I	ATGCAT	1 cut(s)	702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.