Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002990

Physical Location & Seq

Genomic Coordinates Forward (+)

200874 .. 211309

Gene Structure 10436 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G00450.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6708 bp

ATGCAAAGGTATCATGCTGCCAACTGCACTAGTGCAGTCAACAATAGTGCTATAGGTGGTGCATCCGCGAGGGACTCAGGACGAGCTGATTCCTCTTCGATTGGAAACTATTCACTAAACTCCAGGAGACCACCACCGTTGACACCCTACAAGTTGAAGTGTGAAAAGGACGGTCTGAATTCCCGATTAGGGCCGCCTGATTTTCATCCTCCAACATCAAATTCTCCCGAAGAGAATCTGACCAAAGAGTATGTTCAATTTGGATACAAGGAAACTGTTGATGGACTTAAGGAATCCGAAGAAATTATATTGTCCCAGGTCCATACTTTCTCAAAGCCTGTTGTCCATAAGTGCAAAGAGGCAGTCAGAAAGTGTCTTAGAGCTATCAATGAATCTCGTGCACTGAAGCGCAAGGCTGGTCAGGTTTATGGGGTGCCTCTCTCTGGTTCGCTTCTATGTAAGCCTGGATTTCCAGAACAAAGGTCATGTGGGGAGGAGACAAAGAAAAGATGGATCGAGAGTTTATCACAACAGCACAAACGATTGCGCTCTTTGGCTGATAACATTCCTGGATATAGGAGAAAAACCTTATTTGAAGTCCTTATAAGGAACAATGTCCCACTATTGAGAGCTACTTGGTTTATAAAAGTGACTTATCTCAATCAGGTGCGACCTAGTCCTGCTGCTATTTCTTCAGGAACACCTGACAAGACACAAGCTTCTCGGTGTGAGCAATGGACAAAAGATGTTATTGAATATTTGCAATACCTCTTGGATGAACTTTTGTCACGGAATAGCTCATTTCCTGCTCAGCAAACTAGAGATAGGTCACCACAGATGCTTTATACAGGATCAATGCAAAAGAATAGTCCAGCATCAACAAGCCTTTACGGCGAGGAAACATCTCTACATTTTAAATGGTGGTATATGGTGCGTCTTCTACAGTGGCACCATGCTGAAGGGCTTCTTTTTCCTAATCTCATTGTTGATTGGGTTCTCAAGCTCTTACAGGAAAAGGAGATCTTTGAAATTTTGCAGCTGCTACTCCCCATTGTGTATGGTGTTTTAGAGAGTATTGTTCTCTCTCAGACATATGTACAGAGTCTTGTAGCTATTGCTGTCCGTTTCATTCAGGAACCTGCTCCTGGTGGATCTGATCTTGTTGATAACTCTCGAAGAGCTTATACTCTATCTGCTCTAATTGAGATGGTTCGCTACTTGGTACTTGCTGCACCCGACACATTTGTTGCTTCAGATTTCTTTCCTCTTCCTCCTTCCGTAGCAGCATGTGGACCTAATGATGTGAGCTATACGTCAAAGGCTTATGAGAATCTGGAAAAGCTGAGAAGTAACTCTGCGGAGATTTCTGCCCAGTTTCAAGGAAGAGGAGTTCTTTCCCGGTTTGAGTTCCTTTCTTTTGATTATACCATTTCAACCATTCAAAGAAGTGCAGATGATTTGGCAAAGATAGCTAGCGCTGGTTACCCTCAACATAACGTGGCTAAAGCTGTTCAGGCCTTGGATAAAGCTTTGTCGGATGGAGATATCAGAGCTGCTTACAGTTATCTCTTTGAAGACCTTTGCAATGGAGCCGTTGATGAGGCCTGGATTACTGACGTCAGTCCATGTTTAAGATCATCCCTTAGATGGATTGGCGCTATTAGCACATCCTTTGTTTGCTCCGTTTTTTTCCTTATAGAATGGGCTACATGTGATTTTAGAGATTTCCGTGCTGGTGTGCCTAAAGATATCAAGTTCTCTGGCAGAAAAGATTGTTCTCAGGTGTATTTAGTCATTCAGCTTCTGAAGCAAAAGATTCTGGGTGGAGAATTCACAGCTCGCAAAGGAAAGAATTGTCGCAACAATTTTCTTGGTGTTTCTAAACCTAGCGGTTCGATGGATGCATTTGAAAGCCCGGGCCCATTACATGATATCATAGTCTGTTGGATTGATCAGCATGAGGTACACAAGGGAGGAGCAAAGCGCTTGCAATTACTCGTTTTTGAACTTATACGTTCTGGAATCTTTAATCCCATAGCATATGTGAGGCAACTCATAGTTAGCGGGATGATCGATGTGATTCAACCTGCTGTTGATCCTGAAAGGAGAATGAGGCACCATCGCATATTGAAACAGCTACCTGGGTGTTTTGTACATGAAACCTTAGAGGAAGCTCAACTCTTTGGAGGGGATAAGCTTTCCGAGGCTGTGAGAACTTATTCTAATGAACGGCGCCTTCTTCTTCGAGAACTGCTTGTTGAGAAAGGTAAATACTGGAATAATTTAGTTCTCTCAGATCAGAAGTCAAAGAAAATTTCCACTTCCCTTTCGTCTGTTATTTTTCCAAGGGCGTGTAATGCCAAGAGCAATAGTAAGGGACCTCGCAAACATACCAAGAGTAGTGTGGATATTAGAGAACTAAAGGAGCGCATATCAGCTCTACTGCAGTTTCCAGGTATGTCATGTGGTGTGGAAACCCCAGTGCGAGATGAATTCCAAAATAGTGTTAAGAGATCAAGTGGATCTGTGTATAGCAAGATGGATCAACCAGAAGCTACACCAGGGTGTGAAGACTGTAGAAGAGCAAAAAGACCAAAAATGAATGATGAAAAGAGCTCTTGCTATCAAGGGAATTCACCAATTGCATCAGATGAAGAAGATAACTGGTGGATCAAGAAAGGATCGAAAACTGTGGAGTCTTCTCTGAAGGTTGATCCTCAGATAGAGATAACTAAACAAGTACCACGAGGTAGGCAGAAGATGGCGCGTAAGACACAGTCGCTGGCTCAACTACAAGCTGCTAGAATTGAAGGTAGCCAGGGCGCTTCAACGAGTCATGTTTGTGATAATAAAGTAAGCTGTCCTCATCATGGCCCTGGAGTGGAAGGAGAAAATCAGAAGGTGGTTGATGTGTTTAGAACTTCCACCCCTGTGGATATGGTATCTGTTGGAAACTCTTTGAAGCAGCTACAGTTTGTTGATAAGCGATCTATTGCAGTTTGGCTTACTACTGCAGTTCGGCAACTTGTTGAAGAGCCCCAAAAGAGCAGTGTGAGAGTTGGGCAGTTCAATAGAGGTGCTCCAGTTGAGGAGAAGAACACAATTAGGTGGAAGCTTGGGGCAGACGAGCTATACTCCATATTATTTCTCTTGGATATCTCTCTTGACTTGGTTTCAGCGGTTAAATTTCTTCTTTGGTTATTGCCGAAGGCCAACAGTACCCCAAGTTTTGCCGTACAGGGTGGAAGAAACCTTGTAACTGTACCAAGGAATGTTGAAAACAACATGTGTGAAATAGGCGAGGCGATTCTAGTATCATCACTGAGAAGGTATGAAAACATTCTACTTTCAGCTGATCTTGTTCCTGAGGCCATGACAGCTTTGATGAACCGTGCTGCATCACTTATGTCTAGTAATGGAAAAATTTCTGGATCAGCAGCCTTAGTTTATACTCGCTATATTTTGAAAAGATATGGAAGCCTCCCCAGTGTTGTGGAATGGCATAACAATTTCAAAGCAACATCCGAAAAGAAGCTACTTTCTGAACTAGATCATACGCGATCAGGAAATGGTGAGTATGGGAACCCCCTCGGGGTTCCAGCAGGAGTAGATAATCCAGATGACTATTTACGTAAAAAAATCAGCATTGGCGGTGCACGTCCTTCAAGAGTGGGTTTGAGTATGCGAGATGTTTTGCAACGACATGTTGAAGAGGCGACTCATTATCTCAAAAAACTCATTGGGACTGGTACTATGAAAGCCTCTCTCGCTGAGAAAAATGATGACGGGTATCAGGTGGCTCAACAAATTGTAGTTGGACTAATGGACTGCATTAGACAGACTGGCGGTGCAGCTCAAGAGGGTGATCCCTCTTTGGTTTCTTCTGCGGTTTCTGCGATTATAAACAGTGTAGGCCTTTCAGTGGCAAGGATTACAGATTTCTCTTTGGGAAACATTTATCAGAATCATCCTTCTGGCGTTGATTCATCTAATATTGCACGATACATTTTACGAATCCATATAACCTGTCTGTGCCTTCTTAAGGAAGCTCTTGGAGAGCGTCAAAGCCGAGTGTTTGAAATAGCACTTGCAACAGAAAGTTCCACTGCGCTTACTGGAGTTTTTGCTCCTGTGAAGGGATCACGAGGTCAGCATCAGCTATCTCCTGAATCCTATGATTCAAATGCAAACAATTCAACAATCGATATGTCGAATGGTACTGGAAAAATGGCGCTGAGCAGAGCGACAAAAATTACTGCAGCTGTATCTGCACTTGTTATAGGTTCTATCACACATGGTGTTATAACCCTTGAGAGGATTGTTGGTCTACTGAGACTAAAGGATTACTTAGATTTCGTTCAGTTTGTAAGGCGTACAAAATCGAGTTCTAATGGCAGTGCTAGATCCATGGGAGCCTCTAAAGTGGAAAGCCCTATTGAAGTGTATGTACATTGGTTCAGACTCCTTGTTGGTAACTGTAAAACTGTTTCGGAAGGGCTGGTTTTGGAGCTTGTGGGAGAATCTTCCGTGGTGGCTATATCACGCATGCAGCGTATGCTTCCGCTGAAATTGGTCTTCCCACCAGCCTATTCAATTATTGCGTTTGTTCTGTGGAGGCCTTTTGTTTCAAATAGTAACTCTAACTCCAGCGTCCATGAAGACACTCACCGCCTTTATCAGTCTTTGACAATGGCCTTCCATGATGTTATTAAGCATCTTCCTTTCCGAGACGTGTGCTTCAGAGACACCCAGGGACTTTATGAACTGATAGTTGCTGATTCCACAGATGCTGAATTTGCGTCGGTGTTTGAATCGCATGGATTGGATATGCACTTGAAATCTGTGGCCTTTGCTCCTCTTCGAGCGCGTCTTTTCTTAAATTCCCTAATTGATTGTAAGGTGCCATCCTCTGGTTATTCCCATGAAGGAGTTAGTGAAGCAAAAAACCGACATCAGGGAAATGGAACAAAGCTTGTGGACAAGCTTGTATCTGTATTAGATTGCCTGCAGCCTGCAAAATTTCACTGGCAGTGGGTTGAACTCAGGCTGCTTCTAAACGAGCAAGCACTTGCTGAGAAACTCGAAAATCATGATATGCCTTTGACAGATGCAATACGATCTTCCTGTCCTACCTCTGAGAAGCCTGATGCCTCTGAGAATGAGAAAAATTTCATTCAAATCCTCCTCACAAGGTTATTGGTTAGACCTGATGCGGTCCCTCTTTTCTCAGAAGTGGTTCATCTCTTCGGTAGATCGGTTGAGGATTCAATGTTGAAGCAAGCTGAATGGTTTCTAGCAGGCCAAGATGTTCTTTTTGGACGAAAAACAATCAGACAAAAACTGATTATTGTAGGTGAAAGCAAAGGACTCCCCACGAAACCTCAGTTTTGGAAACCTTGGGGTTGGTGCAACAGCTCTAGTTCCGATCATATTACAGCAAACAAGGCAGGGAAGAAAAGGAAGTTTGAAATTACTTCTATCGAAGAAGGGGAAGTGATTGAGGAAGGTTCAGGTTCAAGAAAGGTATTATTACCCCGGGTATTGGATGAGAATAGTCCCAGTGTTGGCTATGGGATTACAACTGAGAGGGCTTTTGTTCAGCTAGTGCTTCCATGCATAGACCAAAGCTCTGATGAGTCTCGAAGTACCTTTGTGAATGAGTTGGTAAGACAGTTTAGCAATATTGAGCAGCAATTAAGTTCAGTTACCAACCGCAGTACAACAAGCAACAAGCAAATGGGAACTGCTTCTTCTGGGTCTGAGATTTCATCAAATAAAGGAAGTACCCGGAAGGGCCTTCGCGGTGGTAGCCCTAGTTTGGCAAGAAGATCCTCAGCCAATACTACTGACACTTCGCCGCCGCCTTCCCCTGCTGCTTTGAGAGCTTCTATGTCTTTGCGGTTGCAATTTCTTCTAAGATTACTGCCTGTCATCTGCGGGGAACCTTCATTTAAGAACACCAGACATGCACTTGCGTCTACAATAGTTCGTCTACTTGGAAGCAGAGTAGTTTATGAAGATTATGCTGTATGTTCTCCTCGTAGTGAGCTATCAAAGGCAGAAACAGAATCAACAATAGATCCTTCTTCCATGGCAGATCTCTCTAGTGAAGTCTTATTTGACCGGTTACTGTTTGTACTCCATGGGCTGTTAAGCAATCACCAGCCAAAATGGCTAAAGCCAAGGCCTTCCTCTAATGAATCATCCAAAGACTTTACCTTGTTTGATCGCGATGCAGCAGAGAGCTTACAGAATGAGCTTTCGCGGATGCAGTTACCAGACACCATTAGATGGCGGATCCAAGCAGCGATGCCAATCCTCCTTCCTTCTCTACGCTGTTCTCTCTCATGCCAGCCTCATTCTGTTCCGCCAACCGCACTCACACTTGTTCAGCCCTCTGGATCTACTGCTGCTGCTGGAACCAATCAAAGAAACTCCCCTGCCATATCGAAAAGTGGAACTGCAGCAGCACAAGGGAAGCTGAAGCCGACAATGTTGGCACCACATCAACAACAAGAAGCAGACAACACGGATGTGGTTGACCCGTGGACGCTCCTCGAAGATGGGACAAGTTCAGGTCTATCAAGCAGCAACGCTTCAAATAGCAGCGACATGGCCAACCTTCGAGCCACCTGTTGGCTGAAAGGTGCAGTCAGGGTCAGACGGACTGATCTAACTTACGTTGGTTCAGTAGACGACGACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006352 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016592 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:0070013 GO:0071704 GO:0090213 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med12

Protein Analysis

2235

Amino Acids

247.22

Weight (kDa)

8.95

Isoelectric Point (pI)

48.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med12	PF09497	157 - 216	2.3e-17	Transcription mediator complex subunit Med12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00450 AT4G00450 AT4G00450
fragaria_vesca	FvH4_7g14270 FvH4_7g14270 FvH4_7g14270
malus_domestica	MD01G1060600.v1.1 MD07G1137100.v1.1
prunus_persica	Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1
pyrus_communis	pycom01g09080 pycom07g13490
rosa_chinensis	RchiOBHm_Chr1g0355261
rosa_laevigata	RLG00000028166
rosa_multiflora	Rmu_sc0003115.1_g000044
rosa_roxburghii	Rroxscaffold_4G00300550
rosa_rugosa	Rorug01G0245800 Rorug01G0245900 Rorug01G0246000 Rorug01G0246100 Rorug01G0246200 Rorug01G0246200 Rorug01G0246200
rosa_samantha	Rh1AG258300 Rh1BG227900 Rh1CG242000 Rh1DG255700
rosa_wichuraiana	Rw1G022800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	605, 644, 3902, 4304
AatII	GACGTC	1 cut(s)	1620
Acc36I	ACCTGC	2 cut(s)	1147, 2095
AccB1I	GGYRCC	6 cut(s)	433, 948, 2115, 2232, 4900, 6499
AccB7I	CCANNNNNTGG	3 cut(s)	4910, 6291, 6636
AccI	GTMKAC	4 cut(s)	4327, 5978, 5992, 6693
AccII	CGCG	7 cut(s)	68, 2767, 3559, 4867, 5802, 6231, 6265
AcoI	YGGCCR	1 cut(s)	6615
AcuI	CTGAAG	8 cut(s)	425, 678, 978, 1236, 1826, 2726, 4723, 6503
AcyI	GRCGYC	2 cut(s)	1617, 2233
AdeI	CACNNNGTG	1 cut(s)	4298
AfeI	AGCGCT	2 cut(s)	1477, 1985
AflII	CTTAAG	2 cut(s)	287, 4040
AflIII	ACRYGT	4 cut(s)	1709, 3285, 3703, 4731
AgeI	ACCGGT	1 cut(s)	6123
AhlI	ACTAGT	1 cut(s)	29
AjiI	CACGTC	2 cut(s)	3659, 4732
AjuI	GAANNNNNNNTTGG	2 cut(s)	205, 237
AleI	CACNNNNGTG	3 cut(s)	2563, 2930, 6533
AloI	GAACNNNNNNTCC	2 cut(s)	1374, 1406
Alw21I	GWGCWC	4 cut(s)	403, 2618, 3082, 3658
Alw26I	GTCTC	6 cut(s)	121, 491, 4327, 4722, 4737, 5643
Alw44I	GTGCAC	2 cut(s)	399, 3654
AlwNI	CAGNNNCTG	3 cut(s)	1804, 2782, 4790
Ama87I	CYCGRG	3 cut(s)	1915, 3590, 5535
Aor51HI	AGCGCT	2 cut(s)	1477, 1985
ApaI	GGGCCC	1 cut(s)	1922
ApaLI	GTGCAC	2 cut(s)	399, 3654
ArsI	GACNNNNNNTTYG	4 cut(s)	348, 380, 853, 885
AsiGI	ACCGGT	1 cut(s)	6123
Asp700I	GAANNNNTTC	9 cut(s)	109, 963, 2218, 3339, 3424, 5168, 5235, 5289, 5746
AspS9I	GGNCC	9 cut(s)	191, 319, 1292, 1918, 1919, 2378, 2873, 5215, 5794
AsuC2I	CCSGG	6 cut(s)	1399, 1916, 1917, 5536, 5537, 5788
AsuHPI	GGTGA	7 cut(s)	822, 2628, 3584, 3875, 4658, 5366, 6152
AsuNHI	GCTAGC	1 cut(s)	1472
AvaI	CYCGRG	3 cut(s)	1915, 3590, 5535
AvaII	GGWCC	4 cut(s)	319, 1292, 2378, 5215
AxyI	CCTNAGG	1 cut(s)	3366
BaeGI	GKGCMC	3 cut(s)	403, 1922, 3658
BalI	TGGCCA	1 cut(s)	6617
BamHI	GGATCC	1 cut(s)	6297
BanI	GGYRCC	6 cut(s)	433, 948, 2115, 2232, 4900, 6499
BanII	GRGCYC	3 cut(s)	1922, 2618, 3039
BarI	GAAGNNNNNNTAC	4 cut(s)	1168, 1200, 4431, 4463
BauI	CACGAG	3 cut(s)	396, 2745, 4143
BbsI	GAAGAC	6 cut(s)	929, 1581, 2577, 2691, 4567, 4665
Bbv12I	GWGCWC	4 cut(s)	403, 2618, 3082, 3658
BbvCI	CCTCAGC	1 cut(s)	5833
BceAI	ACGGC	4 cut(s)	907, 1577, 2246, 3218
BcgI	CGANNNNNNTGC	2 cut(s)	4716, 4750
BciVI	GTATCC	1 cut(s)	257
BclI	TGATCA	1 cut(s)	1951
BcnI	CCSGG	6 cut(s)	1399, 1916, 1917, 5536, 5537, 5788
BcoDI	GTCTC	6 cut(s)	121, 491, 4327, 4722, 4737, 5643
BcuI	ACTAGT	1 cut(s)	29
BfmI	CTRYAG	9 cut(s)	51, 941, 2444, 2575, 2969, 3012, 4257, 5006, 6462
BfoI	RGCGCY	6 cut(s)	1479, 1659, 1987, 2236, 2826, 4235
BfrI	CTTAAG	2 cut(s)	287, 4040
BfuAI	ACCTGC	2 cut(s)	1147, 2095
BfuI	GTATCC	1 cut(s)	257
BglI	GCCNNNNNGGC	2 cut(s)	891, 6172
BglII	AGATCT	2 cut(s)	1020, 6097
BlpI	GCTNAGC	2 cut(s)	810, 4235
Bme18I	GGWCC	4 cut(s)	319, 1292, 2378, 5215
BmeT110I	CYCGRG	3 cut(s)	1915, 3590, 5535
BmgBI	CACGTC	2 cut(s)	3659, 4732
BmgT120I	GGNCC	9 cut(s)	191, 319, 1292, 1918, 1919, 2378, 2873, 5215, 5794
BmrI	ACTGGG	4 cut(s)	1366, 2474, 3480, 5553
BmtI	GCTAGC	1 cut(s)	1476
BmuI	ACTGGG	4 cut(s)	1366, 2474, 3480, 5553
BoxI	GACNNNNGTC	1 cut(s)	1620
BpiI	GAAGAC	6 cut(s)	929, 1581, 2577, 2691, 4567, 4665
BplI	GAGNNNNNCTC	2 cut(s)	3119, 3151
BpmI	CTGGAG	5 cut(s)	106, 2898, 3066, 4137, 4630
Bpu10I	CCTNAGC	1 cut(s)	5833
Bpu1102I	GCTNAGC	2 cut(s)	810, 4235
BpuEI	CTTGAG	3 cut(s)	983, 3840, 4331
BpuMI	CCSGG	6 cut(s)	1399, 1916, 1917, 5536, 5537, 5788
Bsa29I	ATCGAT	2 cut(s)	2073, 4203
BsaAI	YACGTR	1 cut(s)	3632
BsaBI	GATNNNNATC	2 cut(s)	204, 6314
BsaHI	GRCGYC	2 cut(s)	1617, 2233
BsaI	GGTCTC	1 cut(s)	121
BsaWI	WCCGGW	1 cut(s)	6123
BsaXI	ACNNNNNCTCC	2 cut(s)	4628, 4658
Bse118I	RCCGGY	1 cut(s)	6123
Bse21I	CCTNAGG	1 cut(s)	3366
Bse3DI	GCAATG	2 cut(s)	740, 1591
Bse8I	GATNNNNATC	2 cut(s)	204, 6314
BseCI	ATCGAT	2 cut(s)	2073, 4203
BseJI	GATNNNNATC	2 cut(s)	204, 6314
BseMI	GCAATG	2 cut(s)	740, 1591
BseRI	GAGGAG	8 cut(s)	509, 1401, 1989, 3104, 4845, 5174, 6027, 6545
BseSI	GKGCMC	3 cut(s)	403, 1922, 3658
BsgI	GTGCAG	7 cut(s)	10, 54, 1215, 1470, 3870, 4254, 6669
Bsh1236I	CGCG	7 cut(s)	68, 2767, 3559, 4867, 5802, 6231, 6265
BshNI	GGYRCC	6 cut(s)	433, 948, 2115, 2232, 4900, 6499
BshTI	ACCGGT	1 cut(s)	6123
BshVI	ATCGAT	2 cut(s)	2073, 4203
BsiHKAI	GWGCWC	4 cut(s)	403, 2618, 3082, 3658
BsiHKCI	CYCGRG	3 cut(s)	1915, 3590, 5535
BsiSI	CCGG	5 cut(s)	1399, 1916, 5536, 5788, 6124
BslFI	GGGAC	9 cut(s)	86, 298, 602, 2391, 3757, 4767, 5201, 5541, 6580
BsmAI	GTCTC	6 cut(s)	121, 491, 4327, 4722, 4737, 5643
BsmBI	CGTCTC	1 cut(s)	4722
BsmFI	GGGAC	9 cut(s)	86, 298, 602, 2391, 3757, 4767, 5201, 5541, 6580
Bso31I	GGTCTC	1 cut(s)	121
BsoBI	CYCGRG	3 cut(s)	1915, 3590, 5535
Bsp120I	GGGCCC	1 cut(s)	1918
Bsp1286I	GDGCHC	6 cut(s)	403, 1922, 2618, 3039, 3082, 3658
Bsp1407I	TGTACA	3 cut(s)	1096, 2152, 4447
Bsp1720I	GCTNAGC	2 cut(s)	810, 4235
Bsp19I	CCATGG	3 cut(s)	4407, 6090, 6142
Bsp68I	TCGCGA	1 cut(s)	6231
BspDI	ATCGAT	2 cut(s)	2073, 4203
BspFNI	CGCG	7 cut(s)	68, 2767, 3559, 4867, 5802, 6231, 6265
BspHI	TCATGA	1 cut(s)	5089
BspMAI	CTGCAG	5 cut(s)	2448, 3016, 4261, 5010, 6466
BspMI	ACCTGC	2 cut(s)	1147, 2095
BspOI	GCTAGC	1 cut(s)	1476
BspQI	GCTCTTC	3 cut(s)	1171, 2575, 3027
BspT107I	GGYRCC	6 cut(s)	433, 948, 2115, 2232, 4900, 6499
BspTI	CTTAAG	2 cut(s)	287, 4040
BspTNI	GGTCTC	1 cut(s)	121
BsrDI	GCAATG	2 cut(s)	740, 1591
BsrFI	RCCGGY	1 cut(s)	6123
BsrGI	TGTACA	3 cut(s)	1096, 2152, 4447
BssAI	RCCGGY	1 cut(s)	6123
BssNI	GRCGYC	2 cut(s)	1617, 2233
BssSI	CACGAG	3 cut(s)	396, 2745, 4143
BssT1I	CCWWGG	8 cut(s)	1518, 2345, 3266, 4407, 5396, 6090, 6142, 6182
Bst2BI	CACGAG	3 cut(s)	396, 2745, 4143
BstACI	GRCGYC	2 cut(s)	1617, 2233
BstAFI	CTTAAG	2 cut(s)	287, 4040
BstAPI	GCANNNNNTGC	1 cut(s)	4555
BstAUI	TGTACA	3 cut(s)	1096, 2152, 4447
BstBAI	YACGTR	1 cut(s)	3632
BstDSI	CCRYGG	5 cut(s)	4407, 4527, 6090, 6142, 6545
BstEII	GGTNACC	2 cut(s)	828, 1481
BstENI	CCTNNNNNAGG	1 cut(s)	4040
BstFNI	CGCG	7 cut(s)	68, 2767, 3559, 4867, 5802, 6231, 6265
BstH2I	RGCGCY	6 cut(s)	1479, 1659, 1987, 2236, 2826, 4235
BstMAI	GTCTC	6 cut(s)	121, 491, 4327, 4722, 4737, 5643
BstNSI	RCATGY	6 cut(s)	1290, 1713, 3289, 3707, 4549, 5969
BstPAI	GACNNNNGTC	1 cut(s)	1620
BstPI	GGTNACC	2 cut(s)	828, 1481
BstSFI	CTRYAG	9 cut(s)	51, 941, 2444, 2575, 2969, 3012, 4257, 5006, 6462
BstSLI	GKGCMC	3 cut(s)	403, 1922, 3658
BstSNI	TACGTA	1 cut(s)	3632
BstUI	CGCG	7 cut(s)	68, 2767, 3559, 4867, 5802, 6231, 6265
BstV2I	GAAGAC	6 cut(s)	929, 1581, 2577, 2691, 4567, 4665
BstX2I	RGATCY	9 cut(s)	1020, 1151, 2522, 4403, 5828, 6079, 6097, 6297, 6401
BstXI	CCANNNNNNTGG	2 cut(s)	2932, 3493
BstYI	RGATCY	9 cut(s)	1020, 1151, 2522, 4403, 5828, 6079, 6097, 6297, 6401
Bsu15I	ATCGAT	2 cut(s)	2073, 4203
Bsu36I	CCTNAGG	1 cut(s)	3366
BsuI	GTATCC	1 cut(s)	257
BsuTUI	ATCGAT	2 cut(s)	2073, 4203
BtgI	CCRYGG	5 cut(s)	4407, 4527, 6090, 6142, 6545
BtgZI	GCGATG	3 cut(s)	2105, 6246, 6323
BtrI	CACGTC	2 cut(s)	3659, 4732
BtsI	GCAGTG	4 cut(s)	3055, 4104, 4402, 5036
BtuMI	TCGCGA	1 cut(s)	6231
BveI	ACCTGC	2 cut(s)	1147, 2095
CaiI	CAGNNNCTG	3 cut(s)	1804, 2782, 4790
CciI	TCATGA	1 cut(s)	5089
Cfr10I	RCCGGY	1 cut(s)	6123
Cfr13I	GGNCC	9 cut(s)	191, 319, 1292, 1918, 1919, 2378, 2873, 5215, 5794
Cfr9I	CCCGGG	2 cut(s)	1915, 5535
ClaI	ATCGAT	2 cut(s)	2073, 4203
CseI	GACGC	7 cut(s)	923, 4049, 4639, 4788, 4856, 5964, 6559
CspAI	ACCGGT	1 cut(s)	6123
CspCI	CAANNNNNGTGG	4 cut(s)	120, 155, 4956, 4991
DinI	GGCGCC	1 cut(s)	2234
DraI	TTTAAA	1 cut(s)	916
DraIII	CACNNNGTG	1 cut(s)	4298
EaeI	YGGCCR	1 cut(s)	6615
EciI	GGCGGA	2 cut(s)	6310, 6357
Ecl136II	GAGCTC	1 cut(s)	2616
Eco105I	TACGTA	1 cut(s)	3632
Eco130I	CCWWGG	8 cut(s)	1518, 2345, 3266, 4407, 5396, 6090, 6142, 6182
Eco147I	AGGCCT	5 cut(s)	1517, 1604, 3915, 4618, 6187
Eco24I	GRGCYC	3 cut(s)	1922, 2618, 3039
Eco31I	GGTCTC	1 cut(s)	121
Eco32I	GATATC	4 cut(s)	1546, 1750, 1933, 3157
Eco47I	GGWCC	4 cut(s)	319, 1292, 2378, 5215
Eco47III	AGCGCT	2 cut(s)	1477, 1985
Eco53kI	GAGCTC	1 cut(s)	2616
Eco57I	CTGAAG	8 cut(s)	425, 678, 978, 1236, 1826, 2726, 4723, 6503
Eco81I	CCTNAGG	1 cut(s)	3366
Eco88I	CYCGRG	3 cut(s)	1915, 3590, 5535
Eco91I	GGTNACC	2 cut(s)	828, 1481
EcoICRI	GAGCTC	1 cut(s)	2616
EcoNI	CCTNNNNNAGG	1 cut(s)	4040
EcoO109I	RGGNCCY	2 cut(s)	2378, 5794
EcoO65I	GGTNACC	2 cut(s)	828, 1481
EcoRI	GAATTC	4 cut(s)	178, 1829, 2492, 2632
EcoRV	GATATC	4 cut(s)	1546, 1750, 1933, 3157
EcoT14I	CCWWGG	8 cut(s)	1518, 2345, 3266, 4407, 5396, 6090, 6142, 6182
EcoT22I	ATGCAT	2 cut(s)	1906, 5618
EcoT38I	GRGCYC	3 cut(s)	1922, 2618, 3039
EgeI	GGCGCC	1 cut(s)	2234
EheI	GGCGCC	1 cut(s)	2234
ErhI	CCWWGG	8 cut(s)	1518, 2345, 3266, 4407, 5396, 6090, 6142, 6182
Esp3I	CGTCTC	1 cut(s)	4722
FalI	AAGNNNNNCTT	8 cut(s)	951, 983, 1007, 1039, 4331, 4363, 5295, 5327
FaqI	GGGAC	9 cut(s)	86, 298, 602, 2391, 3757, 4767, 5201, 5541, 6580
FauI	CCCGC	2 cut(s)	2057, 5930
FauNDI	CATATG	2 cut(s)	1093, 2041
FbaI	TGATCA	1 cut(s)	1951
FblI	GTMKAC	4 cut(s)	4327, 5978, 5992, 6693
FriOI	GRGCYC	3 cut(s)	1922, 2618, 3039
GsuI	CTGGAG	5 cut(s)	106, 2898, 3066, 4137, 4630
HaeII	RGCGCY	6 cut(s)	1479, 1659, 1987, 2236, 2826, 4235
HapII	CCGG	5 cut(s)	1399, 1916, 5536, 5788, 6124
HgaI	GACGC	7 cut(s)	923, 4049, 4639, 4788, 4856, 5964, 6559
Hin1I	GRCGYC	2 cut(s)	1617, 2233
HincII	GTYRAC	3 cut(s)	40, 141, 6541
HindII	GTYRAC	3 cut(s)	40, 141, 6541
HindIII	AAGCTT	6 cut(s)	717, 1527, 2195, 3113, 4970, 4982
HpaII	CCGG	5 cut(s)	1399, 1916, 5536, 5788, 6124
HphI	GGTGA	7 cut(s)	822, 2628, 3584, 3875, 4658, 5366, 6152
Hpy99I	CGWCG	2 cut(s)	4804, 6701
HpyCH4IV	ACGT	8 cut(s)	1313, 1497, 1617, 2014, 3631, 3658, 4731, 6681
HpySE526I	ACGT	8 cut(s)	1313, 1497, 1617, 2014, 3631, 3658, 4731, 6681
Hsp92I	GRCGYC	2 cut(s)	1617, 2233
KasI	GGCGCC	1 cut(s)	2232
Ksp22I	TGATCA	1 cut(s)	1951
LguI	GCTCTTC	3 cut(s)	1171, 2575, 3027
MaeII	ACGT	8 cut(s)	1313, 1497, 1617, 2014, 3631, 3658, 4731, 6681
MfeI	CAATTG	1 cut(s)	2640
MflI	RGATCY	9 cut(s)	1020, 1151, 2522, 4403, 5828, 6079, 6097, 6297, 6401
MhlI	GDGCHC	6 cut(s)	403, 1922, 2618, 3039, 3082, 3658
MlsI	TGGCCA	1 cut(s)	6617
MluNI	TGGCCA	1 cut(s)	6617
Mly113I	GGCGCC	1 cut(s)	2233
MlyI	GAGTC	8 cut(s)	68, 1111, 2705, 2842, 3712, 4455, 5361, 5645
MmeI	TCCRAC	5 cut(s)	236, 1515, 1925, 2929, 3796
Mox20I	TGGCCA	1 cut(s)	6617
Mph1103I	ATGCAT	2 cut(s)	1906, 5618
MroXI	GAANNNNTTC	9 cut(s)	109, 963, 2218, 3339, 3424, 5168, 5235, 5289, 5746
MscI	TGGCCA	1 cut(s)	6617
MslI	CAYNNNNRTG	4 cut(s)	2563, 2841, 2930, 6533
Msp20I	TGGCCA	1 cut(s)	6617
MspA1I	CMGCKG	6 cut(s)	1039, 3179, 3353, 4262, 4564, 6705
MspCI	CTTAAG	2 cut(s)	287, 4040
MspI	CCGG	5 cut(s)	1399, 1916, 5536, 5788, 6124
MunI	CAATTG	1 cut(s)	2640
MvnI	CGCG	7 cut(s)	68, 2767, 3559, 4867, 5802, 6231, 6265
NarI	GGCGCC	1 cut(s)	2233
NciI	CCSGG	6 cut(s)	1399, 1916, 1917, 5536, 5537, 5788
NcoI	CCATGG	3 cut(s)	4407, 6090, 6142
NdeI	CATATG	2 cut(s)	1093, 2041
NheI	GCTAGC	1 cut(s)	1472
NmeAIII	GCCGAG	1 cut(s)	4094
NmuCI	GTSAC	3 cut(s)	649, 786, 828
NruI	TCGCGA	1 cut(s)	6231
NsiI	ATGCAT	2 cut(s)	1906, 5618
NspI	RCATGY	6 cut(s)	1290, 1713, 3289, 3707, 4549, 5969
OliI	CACNNNNGTG	3 cut(s)	2563, 2930, 6533
PaeI	GCATGC	1 cut(s)	4549
PagI	TCATGA	1 cut(s)	5089
PasI	CCCWGGG	1 cut(s)	4750
PceI	AGGCCT	5 cut(s)	1517, 1604, 3915, 4618, 6187
PciI	ACATGT	3 cut(s)	1709, 3285, 3703
PciSI	GCTCTTC	3 cut(s)	1171, 2575, 3027
PcsI	WCGNNNNNNNCGW	1 cut(s)	4549
PdmI	GAANNNNTTC	9 cut(s)	109, 963, 2218, 3339, 3424, 5168, 5235, 5289, 5746
PflFI	GACNNNGTC	2 cut(s)	675, 2776
PflMI	CCANNNNNTGG	3 cut(s)	4910, 6291, 6636
PfoI	TCCNGGA	2 cut(s)	122, 568
PinAI	ACCGGT	1 cut(s)	6123
PleI	GAGTC	8 cut(s)	68, 1110, 2704, 2841, 3712, 4455, 5361, 5644
PluTI	GGCGCC	1 cut(s)	2236
PpsI	GAGTC	8 cut(s)	68, 1110, 2704, 2841, 3712, 4455, 5361, 5644
Ppu21I	YACGTR	1 cut(s)	3632
PpuMI	RGGWCCY	1 cut(s)	2378
PscI	ACATGT	3 cut(s)	1709, 3285, 3703
PshAI	GACNNNNGTC	1 cut(s)	1620
PsiI	TTATAA	4 cut(s)	605, 644, 3902, 4304
Psp124BI	GAGCTC	1 cut(s)	2618
Psp5II	RGGWCCY	1 cut(s)	2378
PspEI	GGTNACC	2 cut(s)	828, 1481
PspOMI	GGGCCC	1 cut(s)	1918
PspPI	GGNCC	9 cut(s)	191, 319, 1292, 1918, 1919, 2378, 2873, 5215, 5794
PspPPI	RGGWCCY	1 cut(s)	2378
PstI	CTGCAG	5 cut(s)	2448, 3016, 4261, 5010, 6466
PstNI	CAGNNNCTG	3 cut(s)	1804, 2782, 4790
PsuI	RGATCY	9 cut(s)	1020, 1151, 2522, 4403, 5828, 6079, 6097, 6297, 6401
PsyI	GACNNNGTC	2 cut(s)	675, 2776
PvuII	CAGCTG	4 cut(s)	1039, 3353, 4262, 6705
RruI	TCGCGA	1 cut(s)	6231
RseI	CAYNNNNRTG	4 cut(s)	2563, 2841, 2930, 6533
SacI	GAGCTC	1 cut(s)	2618
SapI	GCTCTTC	3 cut(s)	1171, 2575, 3027
Sau96I	GGNCC	9 cut(s)	191, 319, 1292, 1918, 1919, 2378, 2873, 5215, 5794
SchI	GAGTC	8 cut(s)	68, 1111, 2705, 2842, 3712, 4455, 5361, 5645
SduI	GDGCHC	6 cut(s)	403, 1922, 2618, 3039, 3082, 3658
SfcI	CTRYAG	9 cut(s)	51, 941, 2444, 2575, 2969, 3012, 4257, 5006, 6462
SfoI	GGCGCC	1 cut(s)	2234
SinI	GGWCC	4 cut(s)	319, 1292, 2378, 5215
SmaI	CCCGGG	2 cut(s)	1917, 5537
SmiMI	CAYNNNNRTG	4 cut(s)	2563, 2841, 2930, 6533
SmlI	CTYRAG	5 cut(s)	287, 998, 3855, 4040, 4310
SmoI	CTYRAG	5 cut(s)	287, 998, 3855, 4040, 4310
SnaBI	TACGTA	1 cut(s)	3632
SpeI	ACTAGT	1 cut(s)	29
SphI	GCATGC	1 cut(s)	4549
SrfI	GCCCGGGC	1 cut(s)	1917
SseBI	AGGCCT	5 cut(s)	1517, 1604, 3915, 4618, 6187
SspDI	GGCGCC	1 cut(s)	2232
SspI	AATATT	3 cut(s)	758, 3994, 5683
SstI	GAGCTC	1 cut(s)	2618
StuI	AGGCCT	5 cut(s)	1517, 1604, 3915, 4618, 6187
StyI	CCWWGG	8 cut(s)	1518, 2345, 3266, 4407, 5396, 6090, 6142, 6182
TaiI	ACGT	8 cut(s)	1316, 1500, 1620, 2017, 3634, 3661, 4734, 6684
TatI	WGTACW	5 cut(s)	1096, 2152, 4447, 5717, 6136
TauI	GCSGC	3 cut(s)	196, 5860, 5863
TseFI	GTSAC	3 cut(s)	649, 786, 828
Tsp45I	GTSAC	3 cut(s)	649, 786, 828
TspGWI	ACGGA	8 cut(s)	805, 1112, 1267, 1672, 1718, 4516, 6545, 6679
TspMI	CCCGGG	2 cut(s)	1915, 5535
Tth111I	GACNNNGTC	2 cut(s)	675, 2776
Van91I	CCANNNNNTGG	3 cut(s)	4910, 6291, 6636
Vha464I	CTTAAG	2 cut(s)	287, 4040
VneI	GTGCAC	2 cut(s)	399, 3654
VpaK11BI	GGWCC	4 cut(s)	319, 1292, 2378, 5215
XagI	CCTNNNNNAGG	1 cut(s)	4040
XceI	RCATGY	6 cut(s)	1290, 1713, 3289, 3707, 4549, 5969
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2401
XmaI	CCCGGG	2 cut(s)	1915, 5535
XmiI	GTMKAC	4 cut(s)	4327, 5978, 5992, 6693
XmnI	GAANNNNTTC	9 cut(s)	109, 963, 2218, 3339, 3424, 5168, 5235, 5289, 5746
ZraI	GACGTC	1 cut(s)	1618
Zsp2I	ATGCAT	2 cut(s)	1906, 5618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.