MD07G1137100.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002990
Physical Location & Seq
Forward (+)
19984884 .. 19998132
13249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1137100.v1.1.491

Sequence Viewer

Length: 6774 bp
ATGCAAAGGTATCCTCCCACTGGCTGCACTAGTGCAGTTAACAACACAACAATAGGTGGGACATCAGGGAGGGATTCTACCCGAGCTGAATCGGCTGCATTGCCTAACTTACCTCTAGCTTCTAGGCGCACATCGCAGTTAAACCCTTACAAATTGAAGTGTGATAAAGACCCTTTGAATACCCGACTTGGGCCACCTGACTTTCACCCCCAAACGCCAAATTGCCCAGAGGAGACTCTTACCAAGGAATACGTGCAAGCTGGATACAGAGAGACTGTTGAAGGAATTGAGGAATCTAGAGAGGTTTCTCTGAGTCAGGCTCAAGCTTTTAGCAAGCTTCTTGTCTTCAGGTGCAAAGAGGCAATCAAAAAACGTTTTAGGGCTATCAATGAATCCCGGGCTCAAAAGAGGAAGGCTGGTCAAGTGTACGGTGTTCCTCTTGTTGATTCATTGTTGTCAAAGCCAGGTGTTTTTCCTGAACAAAAGCCCTGTGGTGAAGAGTTGCGAAAAAAATGGATTGAGGGTCTGTCACAACAACACAAGCGCTTGCGTTCATTGGCTGATCATGTCCCTCATGGGTATAGGAAAAGATCTTTGTTTGAAGTTCTTACAAGGAATAATGTTCCATTGCTGAGAGCAACTTGGTTTATTAAAGTAACTTATCTTAATCAGGTCAGGCCAGGTTCTACCAGTTTATCATCTGGGACATCTGATAAACTTCAGTTGTCCCGCACAGAGCTTTGGACAAAAGATATTATTGAGTATCTGCAGTACCTTCTCGATGAGTTATTCTCGAGAAATAAATCTCATTCCAGTTCAAACAACAGAGATAGATCACCACAAACTTTATATACTGCGTCACTGCCACTAAGGAGTGATCCAGCATCAGCAGTCCTTGATGGTGAGGAGCCTTCCGTACATTTCAAGTGGTGGTATGTTGTGCGGCTTCTGCAATGGCATCATGCCGAGGGGCTGCTTCTTCCTACACTCATCATTGAGTGGGTTCTAAGTCAACTACAGGATAAAGAATTGCTTGAGATTATGCAGTTGTTTTTGCCTATTATTTATGGTGTTCTGGAAACTGTTGTTTTATCTCAAACATATGTGCGCAATCTTGTGAGAGTAGCTGTTCGTTTCATTGCCGAACCTTCTCAAGGTGGATCAGATCTTGTAGATAACTCCCGTAGGGGGTACATAGTGTCTTCTTTGGTTGAGATGCTTCGCTATTTAATACTTGCTGTGCCTGATACGTTTGTAGCTTTGGATTGCTTTCCTCTACCATCCTCTGTAGTTTCCTATGTTTCAAAAGATGGATTACCAAAGATATCTGAGGATGCAAGAAAGATTAAAAATGTTTCAGCAGAAGTTGCTTCTGCAGTAAGAAGCAAAGTTTTTGATACTCAATATCAGTCCCTGGCTTTTGATCGTATTGTTTCATCTATTCAGAAATGTGCTGAAAATCTTGCAAAGGCTGCAAGGCCTAGCTATCCAGGTCATAGTATAGCTAAAGCTGTACAGGCCCTAGATAGATCTCTTGTGCAGGGAGATGTTCGAGGGGCTTATAGATTTCTGTTTGAAGATCCTTGTGATGGAGTTGCAAGTGAAAGTTGGATTGCAGGAGTTAGTCCATGTTTACGAACTTCATTGAAGTGGATTGGGACTGCAAACTTGTCATTTGTTTGCTCTGTATTTTTCCTCTGTGAGTGGGCAACATGTGATTTCAGGGATTTTAGGACTGCCCCACCTTGTGAGCTGAAATTTACTGGCAGAAAGGATTTCTCTCAGGTATTTGTTGTTACTAGGCTTTTGAAGCTAAAGATAAGAGATTTGCAAAGTTCACCTCATCGTAAGAATGACAGCGTCCTTGGAGTCAGTAGTGTTGCAAAAGGTTCTACCCAGCAGAACAATTTTCCTGTCAGCAGAGTTTCCTTGGGAAATTCCTGTGAGGTTAAACCAAAAAATGTTGATCAAAGAAGTATGAAATCGTCAAATATATTCGAGAGCCCTGGTCCTTTACACGATATCATAGTGTGTTGGATTGATCAACATGAAGCAGGCAAAGGAGAAGGTTTGAAGCGCCTTCAACTACTTCTAATTGAACTTATCCGGTGTGGTATTTTTAATCCTCATGCTTATGTTAGGCAGCTGATAGTTAGTGGGATTATGGATATGAATGGACTGGTGGTTGAAGTAGACCGGCGGAAGAGGCATTTTCGTATCTTGAAGCTGTTGCCTGGGCTCTTTATGCGTGATGCTTTGGAAGAAGCAGGGATTGCCGAAGGGCCACAACTTTCAGAGGCCATGAACTTCTACATAACCGAGCGCCGCCTAATACTTCGTGGGCTTCTCTCTGATCAAAATAAAAATGTGTCTGTTCTGAAGCAAAAGAATTATCCAATTCCTGGAAAAGATGGTGGTTTGCCAGTAAAAGTGGCCAGTAAAAGTGGCAAGACTGATGCTGATGTCGAAGAACTAAAGGAAGCAATATCAGTTCTTTTGCAACTGCCAAATAGTTCATCCTCAACAACGGATACAGGGCTTGATGAATCCCACGGGAGTGTTAAGAGGCCTTTTGGGTCAATCTATAACAAGATGGATCTGGGAGAAGGCACCCCTGGGTGTGAAGAATGCAGAAGAGCAAAGAGGCAAAAAGTGAGTGGCGAAAGGAGCTCATGCATCCAAGGAAAATTTTCAATTCCTTCAGATGATGAAGATACCTGGTGGATGAGGAAGAGACCTAAGTCCTTGGAGCCATTGAAGGTTGATCCACCTGTTAAATCAACCAAACAGGTCTCTAGGAACCGGCAGAAGATAGTTCGTAAAACTCAAAGTCTAGCTCAACTTGCAGCTGCTAGGATTGAAGGTAGCCAGGGAGCATCTACAAGTCATGTCTGCAATAATAAAGTAAGCTGTCCACATCATAAAAGTGGAGTTGAGGGGGAAACCCCGAAGTCTATAGATCCAACCAAAATGAACCATGGTGGGGATATTGTTTCAATTGGAAAAGCTTTAAAGCGGCTGCAATTTGTAGAGAAGAGGACAATTACGGTTTGGATAATGACTGTTATCAGGCAGCTTGTTGAAGAGACTGAAAAGACCATTGCGAAGGTTGGCCAATTTGGTAGGACTTTCACATCTGTTGATGATAGGAGCTCCATGCGGTGGAAGCTTGGTGAAGATGAACTTTCTGCTGCACTGTATTTGATGGATGTTTCAAATGATTTAGTTTCAGCAGTCAAGTTTCTCCTTTGGTTGCTGCCAAAGGTTAGTGGCCCTAGTTCTACAATCCATAGTGGTAGGAACATTATGCTGTTGCCCAGAAATGCTGAAAATCAAGTGTGTGAATTGGGGGAGGCATTTCTGGTATCATCACTCCGAAGGTATGAGAACATAATTATTGCTACAGATCTTATTCCAGAAGTCTTGTCTGTTACAATGCATCGTGCTTCAGCCGTAGTGGCATCTAATGGAAGAGTTTCAGGTTCAGCTGCCTTAGCATACAGTCGGTATTTATTGAAGCGATACAGCAATGTAGCTAGTGTCATTGAATGGGAAAAGAGTTTCAAGCCAACATGCGATAAGAGACTTCTTTCTGAACTTGAGTCTGGACAGTCGGTAGATGGGGAGTTGGGGTTTCCGCTTGGTGTTCCAGCCGGAGTCGAAGATCTTGATGATTTTTTCCGCCAAAAGATTAGTGGGGTCCGGTTATCCAGGGTGGGTTTAAACATGAAAGAAATAGTGCAACGAAATGTGAATGTTGATGATGCCTTTCAGTACTTTTTTGGGAAAGAGAGAAAGCTCTTTGCTGCTGGTGCTCCTAAAGGCCCTCCTGTTGATAAATGGGACGATGGATATCAGATATCTCAAAAAGTAATTACAGAACTGATGGACTGCATTAGGCAAACTGGTGGTGCTGCTCAAGAAGGGGATCCATCTTTGGTGTCTTCTGCCATTTCTGCTATTGTGGGTAATGTAGGGCTAACTATAGCAAAGGTTCCAGATTTTAGAGCAGGGGGTAGCTATTCAACCTTTCCATCTGCCACTGATTCCTTGAACTTTGCTAGGCGTATTTTGCGTATCCATATAAGTTGCCTATGCCTCCTTAAGGAAGCTCTTGGAGAGCGCCAAACCCGTGTGTTTGAGGTAGCTCTTGCGACAGAAGCTTGTTCTGCTCTTGCTGGGGTTTTTGCTCCTGGAAAGGCCCCTCGAAATCAATATCACTCCTCCCCTGAATCCCATGATTCCAACACTAATATGTCTAATGACATTTTGAATAGTAAAGTAGTTCTTGGGAGGACTACAAAAGTTGCAGCTGCTGTTTCTGCACTAGTCATCGGGGCGGTTGTTCAAGGAGTTACTAGCTTGGAGAGATTGGTGACTGTATTCAGGTTAAAGGAAAGGTTGGATGTAATTCAATTTGTAAGGAGTAGTAGATCCAACTCTAATGGCAATGCTCGTTCTGCTGGGGCATTTAAGGGTGATAACTCGCTTGAAGTTTATGTGCATTGGTTTAGACTTCTCATTGGAAACTGCAGAACGGTCTCTGATGGATTGGTTGTGGAACTCTTGGGTGAACCCTCTGTAATAGCTCTTTCGAGGTTGCAGCGTATACTCCCTCTTGATTTAGTTTTTCCACCTGCTTTTTCAATATTTGCTTTTGTTATGTGGCGGCCATTCCTTCTTAGCACTAGCTTTGCAGCCCGGGATGATTTTAACCAATCATATCAATCTTTAACAACAGCTATAGGTGATGCTATAAAGCACTCTCCTTTTCGTGATGTGTGTTTGAGAGATAGTCAGGGTTTCTATGATCTTGTAGCTGCAGATGGTAGTGATGCTGAATTTGCAGCCATGCTAGAGTTAAATGGTTCAGACATGCATAAAAAATCCGAGGCTTTTATTCCATTACGTGCAAGGCTTTTTCTAAATGCCATAATGGATTGTAAGATGCCACACTCTTCATTTACACAGGTTGAAGCAAATCAGGTCTCTGGACATGGTGAGTCTAAAGTCCAATTTGCAGAGCGTGAAACAAAGCTTGTAGATAAGCTTGTACATATTTTAGATACCCTGCAACCTGCGAAATTTCATTGGCAGTGGGTAGAGCTTAGGCTCCTTTTGAATGAACAAGCCCTCATTGAAAAACTTAAAGACACTCAAGATGTGTCTTTAGTGGATGCCATTCGGTCGTCATTGCCTAGTCCTGAAAAGGTTGCTGCCTCCGAGAATGAGAAATATTTCATTGAGATCATCCTTACAAGGTTATTAGTCAGACCTGATGCTGCACCCCTTTTCTCGGATGTGGTTCATCTTTTTGGGAGATCACTAGCAGATTCTATGTTGTTGCAGGTCAAATGGTTCCTGGGTGGCTCAGATGTTCTTTATGGACGAAAAACCATTAGACAACGTCTTCTTAATATTGCTGAAAGTAAGGGTCTCTCAATTAAGACCCAATTTTGGAAGCCGTGGGGATGGTGTAGTTATGATGCTGATCCTGTGACAAACAGGGGAGATAAGAGAAAGTTTGAAGTCACCTCCCTTGAAGAGGGTGAGATGGTTGAAGAGGGAACAGACTTGAAAAAGTATGGGAAAGGGGCCACTCAAACATTGGACATTAAAAGCTACAATGTCACTCAGCAGCATGTGACAGAGAGGGCTCTCATTGAATTACTTCTTCCATGTATAGATCAAAGTTCTGATGAGTCACGCAATACATTTGCAAACGATTTGATCAAGCAGTTGGGTAGTATTGAGCAACAAGTTAGTCCAGTTACTCGTGGGACAAATAAGCAAGCAGGACCAACTCCTTCTGGAGTTGAAGGTCCTACAAGCAAAGGAAATAGCCGCAAAGGTATAAGGGGTGGCAGTCCTGGATTAGCTAGGCGTGCAGCAGGAGCAGCTGATTCTGGTCCACCGTCCCCTGCAGCTTTGCGAGCTTCTGTGTCACTTCGGTTGCAGTTGCTCTTAAGATTGCTTCCTATCATTTGCACAGACAGGGAGCCATCTGGCCGGAACATGAGGCATGGGCTTGCCTCTGTAGTACTACGTCTTCTAGGAAACCGAGTTGTGTACGAGGGTGCAGACCTATGCTTCAATATCATGCAGAGTACTTTCTCTAAGAGGGATGCAGAGTCTTCAACAGAAGCTGCCTCTGCAGCTTTTGCAGAATTGTCAAATGAAAGTCTCTTTGACCAATTATTGTTCGTTCTGCATGGGCTGTTAAGCAGTTGCCAGCCAAGTTGGTTGAGGTATACTAAGTCAACGATTGAGGGGGGGAAAGATTTAGCTGCATTTGATCGTGAACTGGCAGATAATTTGCAGAAGGACTTAGATCGTATGCAACTTCCTGAAATTATTCGCTGGCGTATCCAAACTGCAATGCCAGTGGTTATCCCTTCTGTTCAGCGCCTTGTCTCTTGCCAACCACCACCTGTTCCAGATACTGCTTTGGCCGTTCTTCAACCCAGCATATCGGTTTCTGGGTTGCATGCTGGAAGCTCACACCCACCTCAAAGAAATCAGGCTCCTCTGGCGCGGACAGTAACTAACATACCAGGGAAATTTAAACCATTGCCATCACAGGATTATGATATGGATATTGACCCATGGACACTGCTGGAAGATGGTGCAGGTTCAGGCCCATCTTCAAGTAGCAGTGCTTTGATAGGCAGTGCGGACCATGGCAATCTTCGAGCATCTAGCTGGCTCAAGGGGGCAGTGAGGGTGAGACGAAAGGATCTCACATACATTGGTGCTGTGGATGATGACAGCTGA

Protein Analysis

2258

Amino Acids

249.61

Weight (kDa)

9.0

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med12 PF09497 158 - 218 7.1e-20 Transcription mediator complex subunit Med12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4645
Acc16I TGCGCA 1 cut(s) 1109
Acc36I ACCTGC 4 cut(s) 4645, 5086, 5338, 6620
AccB1I GGYRCC 1 cut(s) 2609
AccB7I CCANNNNNTGG 2 cut(s) 2402, 3162
AccI GTMKAC 3 cut(s) 2193, 4609, 6251
AccII CGCG 1 cut(s) 6535
AclI AACGTT 1 cut(s) 373
AcoI YGGCCR 5 cut(s) 2433, 3112, 4670, 6007, 6450
AcsI RAATTY 6 cut(s) 1757, 1936, 2687, 4841, 5084, 6560
AcuI CTGAAG 5 cut(s) 331, 704, 2399, 2685, 3432
AdeI CACNNNGTG 1 cut(s) 1748
AfeI AGCGCT 1 cut(s) 545
AflII CTTAAG 2 cut(s) 4103, 5965
AflIII ACRYGT 1 cut(s) 1712
AhlI ACTAGT 2 cut(s) 29, 4327
AleI CACNNNNGTG 1 cut(s) 2556
Alw21I GWGCWC 3 cut(s) 2672, 3155, 3817
AlwNI CAGNNNCTG 4 cut(s) 1414, 4316, 6146, 6443
Ama87I CYCGRG 4 cut(s) 81, 396, 793, 4701
Aor51HI AGCGCT 1 cut(s) 545
ApoI RAATTY 6 cut(s) 1757, 1936, 2687, 4841, 5084, 6560
Asp700I GAANNNNTTC 5 cut(s) 2689, 3475, 5237, 5670, 6354
AspLEI GCGC 8 cut(s) 129, 546, 1110, 2079, 2325, 4125, 6408, 6535
AsuC2I CCSGG 4 cut(s) 397, 398, 4702, 4703
AvaI CYCGRG 4 cut(s) 81, 396, 793, 4701
AvaII GGWCC 6 cut(s) 2009, 3700, 5798, 5822, 5909, 6676
BaeI ACNNNNGTAYC 2 cut(s) 1239, 1272
BalI TGGCCA 2 cut(s) 2435, 3114
BamHI GGATCC 1 cut(s) 3928
BanI GGYRCC 1 cut(s) 2609
BanII GRGCYC 6 cut(s) 403, 2006, 2241, 2672, 3155, 5659
BarI GAAGNNNNNNTAC 2 cut(s) 6033, 6065
BauI CACGAG 1 cut(s) 5775
BbsI GAAGAC 6 cut(s) 337, 1194, 3936, 5402, 6041, 6126
Bbv12I GWGCWC 3 cut(s) 2672, 3155, 3817
BceAI ACGGC 3 cut(s) 3437, 5449, 6437
BciVI GTATCC 5 cut(s) 21, 257, 2524, 4088, 6377
BclI TGATCA 5 cut(s) 562, 1966, 2041, 2353, 5730
BcnI CCSGG 4 cut(s) 397, 398, 4702, 4703
BcuI ACTAGT 2 cut(s) 29, 4327
BfoI RGCGCY 5 cut(s) 547, 2080, 2326, 4126, 6409
BfrI CTTAAG 2 cut(s) 4103, 5965
BfuAI ACCTGC 4 cut(s) 4645, 5086, 5338, 6620
BfuI GTATCC 5 cut(s) 21, 257, 2524, 4088, 6377
BglI GCCNNNNNGGC 1 cut(s) 3458
BglII AGATCT 5 cut(s) 590, 1165, 1529, 3406, 3664
BmcAI AGTACT 3 cut(s) 3776, 6042, 6109
Bme18I GGWCC 6 cut(s) 2009, 3700, 5798, 5822, 5909, 6676
BmeT110I CYCGRG 4 cut(s) 81, 396, 793, 4701
BoxI GACNNNNGTC 1 cut(s) 2740
BpiI GAAGAC 6 cut(s) 337, 1194, 3936, 5402, 6041, 6126
BplI GAGNNNNNCTC 4 cut(s) 304, 336, 776, 808
BpmI CTGGAG 1 cut(s) 5832
Bpu10I CCTNAGC 2 cut(s) 3493, 5108
BpuEI CTTGAG 7 cut(s) 306, 1055, 1137, 3620, 3903, 5142, 6692
BpuMI CCSGG 4 cut(s) 397, 398, 4702, 4703
BsaAI YACGTR 2 cut(s) 253, 4910
BsaBI GATNNNNATC 2 cut(s) 3852, 5490
BsaI GGTCTC 5 cut(s) 2728, 2797, 4546, 4993, 5441
BsaWI WCCGGW 2 cut(s) 2106, 3702
BsaXI ACNNNNNCTCC 2 cut(s) 3617, 3647
Bse118I RCCGGY 2 cut(s) 2196, 2802
Bse8I GATNNNNATC 2 cut(s) 3852, 5490
BseJI GATNNNNATC 2 cut(s) 3852, 5490
BseMII CTCAG 6 cut(s) 302, 623, 1320, 1796, 5385, 5648
BseRI GAGGAG 4 cut(s) 245, 920, 4213, 6516
BseYI CCCAGC 4 cut(s) 1896, 4178, 4463, 6464
BsgI GTGCAG 9 cut(s) 10, 54, 1559, 3177, 4308, 5268, 5907, 6099, 6648
Bsh1236I CGCG 1 cut(s) 6535
Bsh1285I CGRYCG 1 cut(s) 5189
BshNI GGYRCC 1 cut(s) 2609
BsiEI CGRYCG 1 cut(s) 5189
BsiHKAI GWGCWC 3 cut(s) 2672, 3155, 3817
BsiHKCI CYCGRG 4 cut(s) 81, 396, 793, 4701
BsiSI CCGG 8 cut(s) 397, 2107, 2197, 2803, 3654, 3703, 4702, 6010
BslFI GGGAC 9 cut(s) 73, 554, 712, 718, 1396, 1672, 3857, 5794, 5902
BsmBI CGTCTC 1 cut(s) 6721
BsmFI GGGAC 9 cut(s) 73, 554, 712, 718, 1396, 1672, 3857, 5794, 5902
BsmI GAATGC 1 cut(s) 2633
Bso31I GGTCTC 5 cut(s) 2728, 2797, 4546, 4993, 5441
BsoBI CYCGRG 4 cut(s) 81, 396, 793, 4701
Bsp1286I GDGCHC 7 cut(s) 403, 2006, 2241, 2672, 3155, 3817, 5659
Bsp1407I TGTACA 2 cut(s) 1513, 5053
Bsp19I CCATGG 3 cut(s) 2977, 6605, 6679
BspCNI CTCAG 6 cut(s) 303, 624, 1321, 1795, 5384, 5647
BspFNI CGCG 1 cut(s) 6535
BspMAI CTGCAG 6 cut(s) 771, 1378, 4535, 4825, 5926, 6157
BspMI ACCTGC 4 cut(s) 4645, 5086, 5338, 6620
BspQI GCTCTTC 1 cut(s) 2629
BspT107I GGYRCC 1 cut(s) 2609
BspTI CTTAAG 2 cut(s) 4103, 5965
BspTNI GGTCTC 5 cut(s) 2728, 2797, 4546, 4993, 5441
BsrFI RCCGGY 2 cut(s) 2196, 2802
BsrGI TGTACA 2 cut(s) 1513, 5053
BssAI RCCGGY 2 cut(s) 2196, 2802
BssNAI GTATAC 2 cut(s) 4610, 6252
BssSI CACGAG 1 cut(s) 5775
BssT1I CCWWGG 8 cut(s) 243, 1864, 1929, 2680, 2745, 2977, 6605, 6679
Bst1107I GTATAC 2 cut(s) 4610, 6252
Bst2BI CACGAG 1 cut(s) 5775
BstAFI CTTAAG 2 cut(s) 4103, 5965
BstAPI GCANNNNNTGC 4 cut(s) 1367, 1472, 2273, 6161
BstAUI TGTACA 2 cut(s) 1513, 5053
BstBAI YACGTR 2 cut(s) 253, 4910
BstDSI CCRYGG 5 cut(s) 2551, 2977, 5465, 6605, 6679
BstENI CCTNNNNNAGG 3 cut(s) 1152, 4103, 5543
BstFNI CGCG 1 cut(s) 6535
BstH2I RGCGCY 5 cut(s) 547, 2080, 2326, 4126, 6409
BstHHI GCGC 8 cut(s) 129, 546, 1110, 2079, 2325, 4125, 6408, 6535
BstMCI CGRYCG 1 cut(s) 5189
BstNSI RCATGY 5 cut(s) 1716, 3576, 4879, 5645, 6491
BstPAI GACNNNNGTC 1 cut(s) 2740
BstUI CGCG 1 cut(s) 6535
BstV2I GAAGAC 6 cut(s) 337, 1194, 3936, 5402, 6041, 6126
BstZ17I GTATAC 2 cut(s) 4610, 6252
BsuI GTATCC 5 cut(s) 21, 257, 2524, 4088, 6377
BtgI CCRYGG 5 cut(s) 2551, 2977, 5465, 6605, 6679
BtgZI GCGATG 1 cut(s) 117
BtsI GCAGTG 6 cut(s) 860, 5102, 6611, 6661, 6676, 6723
BveI ACCTGC 4 cut(s) 4645, 5086, 5338, 6620
CaiI CAGNNNCTG 4 cut(s) 1414, 4316, 6146, 6443
CfoI GCGC 8 cut(s) 129, 546, 1110, 2079, 2325, 4125, 6408, 6535
Cfr10I RCCGGY 2 cut(s) 2196, 2802
Cfr9I CCCGGG 2 cut(s) 396, 4701
CseI GACGC 2 cut(s) 846, 1849
CsiI ACCWGGT 1 cut(s) 2717
CspCI CAANNNNNGTGG 2 cut(s) 6366, 6401
DraI TTTAAA 3 cut(s) 3012, 3723, 6565
DraIII CACNNNGTG 1 cut(s) 1748
EaeI YGGCCR 5 cut(s) 2433, 3112, 4670, 6007, 6450
EciI GGCGGA 2 cut(s) 2215, 3671
Ecl136II GAGCTC 2 cut(s) 2670, 3153
Eco130I CCWWGG 8 cut(s) 243, 1864, 1929, 2680, 2745, 2977, 6605, 6679
Eco147I AGGCCT 2 cut(s) 1480, 2569
Eco24I GRGCYC 6 cut(s) 403, 2006, 2241, 2672, 3155, 5659
Eco31I GGTCTC 5 cut(s) 2728, 2797, 4546, 4993, 5441
Eco32I GATATC 4 cut(s) 1326, 2023, 3854, 3861
Eco47I GGWCC 6 cut(s) 2009, 3700, 5798, 5822, 5909, 6676
Eco47III AGCGCT 1 cut(s) 545
Eco53kI GAGCTC 2 cut(s) 2670, 3153
Eco57I CTGAAG 5 cut(s) 331, 704, 2399, 2685, 3432
Eco88I CYCGRG 4 cut(s) 81, 396, 793, 4701
EcoICRI GAGCTC 2 cut(s) 2670, 3153
EcoNI CCTNNNNNAGG 3 cut(s) 1152, 4103, 5543
EcoO109I RGGNCCY 4 cut(s) 1519, 3824, 4201, 5822
EcoRV GATATC 4 cut(s) 1326, 2023, 3854, 3861
EcoT14I CCWWGG 8 cut(s) 243, 1864, 1929, 2680, 2745, 2977, 6605, 6679
EcoT22I ATGCAT 3 cut(s) 2678, 3441, 4881
EcoT38I GRGCYC 6 cut(s) 403, 2006, 2241, 2672, 3155, 5659
ErhI CCWWGG 8 cut(s) 243, 1864, 1929, 2680, 2745, 2977, 6605, 6679
Esp3I CGTCTC 1 cut(s) 6721
FalI AAGNNNNNCTT 6 cut(s) 1017, 1049, 3168, 3200, 4272, 4304
FaqI GGGAC 9 cut(s) 73, 554, 712, 718, 1396, 1672, 3857, 5794, 5902
FauI CCCGC 1 cut(s) 737
FauNDI CATATG 1 cut(s) 1102
FbaI TGATCA 5 cut(s) 562, 1966, 2041, 2353, 5730
FblI GTMKAC 3 cut(s) 2193, 4609, 6251
FriOI GRGCYC 6 cut(s) 403, 2006, 2241, 2672, 3155, 5659
FspI TGCGCA 1 cut(s) 1109
GlaI GCGC 8 cut(s) 128, 545, 1109, 2078, 2324, 4124, 6407, 6534
GsaI CCCAGC 4 cut(s) 1900, 4182, 4467, 6468
GsuI CTGGAG 1 cut(s) 5832
HaeII RGCGCY 5 cut(s) 547, 2080, 2326, 4126, 6409
HapII CCGG 8 cut(s) 397, 2107, 2197, 2803, 3654, 3703, 4702, 6010
HgaI GACGC 2 cut(s) 846, 1849
HhaI GCGC 8 cut(s) 129, 546, 1110, 2079, 2325, 4125, 6408, 6535
Hin6I GCGC 8 cut(s) 127, 544, 1108, 2077, 2323, 4123, 6406, 6533
HinP1I GCGC 8 cut(s) 127, 544, 1108, 2077, 2323, 4123, 6406, 6533
HincII GTYRAC 3 cut(s) 40, 1013, 6261
HindII GTYRAC 3 cut(s) 40, 1013, 6261
HindIII AAGCTT 7 cut(s) 324, 335, 3006, 3167, 4161, 5036, 5048
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 8 cut(s) 397, 2107, 2197, 2803, 3654, 3703, 4702, 6010
HpyCH4IV ACGT 6 cut(s) 252, 373, 1250, 4909, 5407, 6046
HpySE526I ACGT 6 cut(s) 252, 373, 1250, 4909, 5407, 6046
HspAI GCGC 8 cut(s) 127, 544, 1108, 2077, 2323, 4123, 6406, 6533
Ksp22I TGATCA 5 cut(s) 562, 1966, 2041, 2353, 5730
KspAI GTTAAC 1 cut(s) 40
LguI GCTCTTC 1 cut(s) 2629
MabI ACCWGGT 1 cut(s) 2717
MaeII ACGT 6 cut(s) 252, 373, 1250, 4909, 5407, 6046
MfeI CAATTG 1 cut(s) 2997
MhlI GDGCHC 7 cut(s) 403, 2006, 2241, 2672, 3155, 3817, 5659
MlsI TGGCCA 2 cut(s) 2435, 3114
MluNI TGGCCA 2 cut(s) 2435, 3114
MlyI GAGTC 8 cut(s) 229, 322, 1878, 3611, 3666, 5012, 5711, 6140
MmeI TCCRAC 6 cut(s) 1589, 2015, 2987, 4269, 4383, 4461
Mox20I TGGCCA 2 cut(s) 2435, 3114
Mph1103I ATGCAT 3 cut(s) 2678, 3441, 4881
MroXI GAANNNNTTC 5 cut(s) 2689, 3475, 5237, 5670, 6354
MscI TGGCCA 2 cut(s) 2435, 3114
MslI CAYNNNNRTG 5 cut(s) 1648, 2133, 2556, 2925, 4253
Msp20I TGGCCA 2 cut(s) 2435, 3114
MspA1I CMGCKG 6 cut(s) 2146, 2849, 3488, 4313, 5900, 6771
MspCI CTTAAG 2 cut(s) 4103, 5965
MspI CCGG 8 cut(s) 397, 2107, 2197, 2803, 3654, 3703, 4702, 6010
MssI GTTTAAAC 1 cut(s) 3723
MunI CAATTG 1 cut(s) 2997
Mva1269I GAATGC 1 cut(s) 2633
MvnI CGCG 1 cut(s) 6535
NciI CCSGG 4 cut(s) 397, 398, 4702, 4703
NcoI CCATGG 3 cut(s) 2977, 6605, 6679
NdeI CATATG 1 cut(s) 1102
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 9 cut(s) 528, 858, 4375, 5497, 5530, 5629, 5644, 5703, 5943
NsbI TGCGCA 1 cut(s) 1109
NsiI ATGCAT 3 cut(s) 2678, 3441, 4881
NspI RCATGY 5 cut(s) 1716, 3576, 4879, 5645, 6491
OliI CACNNNNGTG 1 cut(s) 2556
PaeI GCATGC 1 cut(s) 6491
PaeR7I CTCGAG 1 cut(s) 793
PaqCI CACCTGC 1 cut(s) 4645
PasI CCCWGGG 1 cut(s) 2615
PceI AGGCCT 2 cut(s) 1480, 2569
PciI ACATGT 1 cut(s) 1712
PciSI GCTCTTC 1 cut(s) 2629
PctI GAATGC 1 cut(s) 2633
PdmI GAANNNNTTC 5 cut(s) 2689, 3475, 5237, 5670, 6354
PflFI GACNNNGTC 2 cut(s) 1859, 5406
PflMI CCANNNNNTGG 2 cut(s) 2402, 3162
PfoI TCCNGGA 3 cut(s) 2401, 4192, 5869
PleI GAGTC 8 cut(s) 229, 321, 1877, 3610, 3665, 5011, 5710, 6139
PmeI GTTTAAAC 1 cut(s) 3723
PpsI GAGTC 8 cut(s) 229, 321, 1877, 3610, 3665, 5011, 5710, 6139
Ppu21I YACGTR 2 cut(s) 253, 4910
PpuMI RGGWCCY 1 cut(s) 5822
PscI ACATGT 1 cut(s) 1712
PshAI GACNNNNGTC 1 cut(s) 2740
Psp124BI GAGCTC 2 cut(s) 2672, 3155
Psp1406I AACGTT 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 5822
PspFI CCCAGC 4 cut(s) 1896, 4178, 4463, 6464
PspPPI RGGWCCY 1 cut(s) 5822
PstI CTGCAG 6 cut(s) 771, 1378, 4535, 4825, 5926, 6157
PstNI CAGNNNCTG 4 cut(s) 1414, 4316, 6146, 6443
PsyI GACNNNGTC 2 cut(s) 1859, 5406
PvuII CAGCTG 6 cut(s) 2146, 2849, 3488, 4313, 5900, 6771
RseI CAYNNNNRTG 5 cut(s) 1648, 2133, 2556, 2925, 4253
SacI GAGCTC 2 cut(s) 2672, 3155
SapI GCTCTTC 1 cut(s) 2629
ScaI AGTACT 3 cut(s) 3776, 6042, 6109
SchI GAGTC 8 cut(s) 229, 322, 1878, 3611, 3666, 5012, 5711, 6140
SduI GDGCHC 7 cut(s) 403, 2006, 2241, 2672, 3155, 3817, 5659
SexAI ACCWGGT 1 cut(s) 2717
Sfr274I CTCGAG 1 cut(s) 793
SinI GGWCC 6 cut(s) 2009, 3700, 5798, 5822, 5909, 6676
SlaI CTCGAG 1 cut(s) 793
SmaI CCCGGG 2 cut(s) 398, 4703
SmiMI CAYNNNNRTG 5 cut(s) 1648, 2133, 2556, 2925, 4253
SpeI ACTAGT 2 cut(s) 29, 4327
SphI GCATGC 1 cut(s) 6491
SseBI AGGCCT 2 cut(s) 1480, 2569
SspI AATATT 3 cut(s) 4650, 5237, 5419
SstI GAGCTC 2 cut(s) 2672, 3155
StuI AGGCCT 2 cut(s) 1480, 2569
StyI CCWWGG 8 cut(s) 243, 1864, 1929, 2680, 2745, 2977, 6605, 6679
TaiI ACGT 6 cut(s) 255, 376, 1253, 4912, 5410, 6049
TaqI TCGA 9 cut(s) 780, 794, 1552, 1998, 2466, 3660, 4207, 4595, 6691
TaqII GACCGA 1 cut(s) 5175
TatI WGTACW 5 cut(s) 1513, 3774, 5053, 6040, 6107
TauI GCSGC 5 cut(s) 946, 2328, 3019, 4672, 5847
TseFI GTSAC 9 cut(s) 528, 858, 4375, 5497, 5530, 5629, 5644, 5703, 5943
Tsp45I GTSAC 9 cut(s) 528, 858, 4375, 5497, 5530, 5629, 5644, 5703, 5943
TspGWI ACGGA 2 cut(s) 904, 2543
TspMI CCCGGG 2 cut(s) 396, 4701
Tth111I GACNNNGTC 2 cut(s) 1859, 5406
Van91I CCANNNNNTGG 2 cut(s) 2402, 3162
Vha464I CTTAAG 2 cut(s) 4103, 5965
VpaK11BI GGWCC 6 cut(s) 2009, 3700, 5798, 5822, 5909, 6676
XagI CCTNNNNNAGG 3 cut(s) 1152, 4103, 5543
XapI RAATTY 6 cut(s) 1757, 1936, 2687, 4841, 5084, 6560
XbaI TCTAGA 1 cut(s) 296
XceI RCATGY 5 cut(s) 1716, 3576, 4879, 5645, 6491
XcmI CCANNNNNNNNNTGG 2 cut(s) 3692, 5605
XhoI CTCGAG 1 cut(s) 793
XmaI CCCGGG 2 cut(s) 396, 4701
XmiI GTMKAC 3 cut(s) 2193, 4609, 6251
XmnI GAANNNNTTC 5 cut(s) 2689, 3475, 5237, 5670, 6354
ZrmI AGTACT 3 cut(s) 3776, 6042, 6109
Zsp2I ATGCAT 3 cut(s) 2678, 3441, 4881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.