Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002990

Physical Location & Seq

Genomic Coordinates Reverse (-)

48168474 .. 48181491

Gene Structure 13018 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G022800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6747 bp

ATGCAAAGGTATCAGCCCACCGGCTTGACTGGTGCAGTTAGTAACAACACAATAGGTGGGGCATCAGGAAGGGATTCTGTCCGAGCTGAATCAGCTACGCTGCCAGCTCACTTACCTTTAAATTCTAGGCGCCCATCTCAAATAGCACCTTACAAATTGAAGTGTGATAAAGACCCTTTGAATGCTCGACTTGGGCCACCTGATTTCCATCCCCAAACCCCAAATTGCCCAGAGGAGACACTTACCAGGGAATATGTGCAATCTGGATACAGGGAAACTGTTGAAGGAATTGAGGAGGCTAGAGAGATATCACTTAGTCAGGCCCAACATTTCAACAAGCCTCTTGTCTTCAGGTGTAGAGAGGCAATCAAAAAACGTCTTAGAGCCATAAATGAATCTCGTGCTCAAAAGAGGAAGGCTGGTCAAGTGTATGGTGTTCCTCTTGCTGATTCACTCTTAACAAAGCCTGGTGTTTTTCCTGAACAAAGGCACTGTGGTGACGACTTACGAAAAAAATGGATTGAGGGTTTATCACAACAACACAAACGCTTGCGTTCATTGGCTGATCATGTTCCTCATGGGTATAGGAAAAGATCTCTATTTGAAGTTCTTACAAGGAATAATGTTCCATTGCTGAGAGCAACTTGGTTTGCTAAAGTCACTTATCTTAATCAGATCCGGCCAGGTCCTTCTAGTATTTCTTCTGGGATACCTGATAAAACTCAGTTGTCTCGCACAGAGCACTGGACCAAAGATATCATTGAATACTTGCAGTACCTTCTCGATGAATTCTTTTCAAGAAACAGTTCACTTCCTGGTTCACACCACAGAGAACGATCACAACAAATGCTTTATGCTGGGTCAGTATCACAACGGAGTGATCCAGCATCATCAGTCCTTGATGGTGACGAGCCTTCCCTACATTTCAAGTGGTGGTATGTGGTGCGACTCCTGCAATGGCATCATACTGAAGGGCTGCTTCTTCCTACACTTGTTATTGAATGGGTTCTACGTCAACTGCAGGAGAAGGAATTGCTTGAGATTGTGCAGTTGCTTTTGCCTATTATATATGGTGTTTTGGAGACTGTTGTCTTATCTCAAACTTATGTGCGCAATCTTGTCGGGACAGCTGTCCGTTTCATACGCGAACCTTCTCAAGGTGGATCCGATCTTGTGGATAACTCCAGAAGGGCGTATACAGTGTCTGCTCTGGTGGAGATGCTTCGTTATTTAATACTGTCTGTACCTGACTCATTTGTAGCTTTGGATTGCTTTCCTCTACCACCATGTGTAGTCTCTTATGCAGTAAACGATGGAAATCTACAGAAGATGTCTGAGGATGTGAGAAAGATTAAAATTGGGTCAGCAGAAGTTGCTTCTGTATTCAGAAGCAAAGCTTTTGATGCTCAGTATCAATCCTTGGCCTTTGATCATGTTGTTTCATCCATTCAGAAGCGTGCTGACAATCTTGAAAAGGCTGCAAGCCCTAGCTATCCAGGCCATAGTATTGCTAAAGCTGTACAGGCCCTAGATAAGTCTCTTGCGCAGGGGGATGTTCTTGGGGTATATAGATTTCTGTTTGAAGATCCTTGCGAGGGAATTATAAATGAAAGTTGGGTTGCAGAAGTTAGTCCTTGCTTGAGAACATCATTGAAATGGATTGGTACTGTGAACTTGTCATTTGTTTGTTCGGTATTTTTCCTTTGTGAGTGGGCAACTTGTGATTTCAGGGATTTTCGAACTGCTCCTCCTTGTGAGCTAAAATTTACTGGCAGAAAAGATTTTTCTCAGGTACACATTGCAACTCGGCTTTTGATGCTAAAGATCCGAGACTTGCAAAGTTCACCTCAACATAAGAATGACAACCCTGCTAAAGGTTCCATTCAGCAGATTAATATTCCTGTCAGAAGTTCCATGGGAAGTTCTTATGAATCTAAAAATAAGTCGAGAAGTGTGCACCAGAGAAGTACAAAATCATCAAATATATTTGAGAGCCCGGGCCCTTTACATGATATCATAGTTTGTTGGATTGATCAACATGATGCAGGCAAAGGAGAAGGTTTGAAGCGCCTTCAATTTCTTGTGATTGAACTCATACGTTCTGGTATTTTTTACCCCCATGTCTATGTTAGACAGCTGATAATTAGTGGCATTATGGATATAAATGGACCAGTGACTGAATCAGACAGAAGGAAGAGGCATTATCAGATCTTGAAGTTGTTGCCTAGGGTCTTTGTGCATGATGCTCTGGAAGAAGCAGTGGTTGCTGAAGGCCCAAAACTTTTGGAGGCTATGAGTTCTTACTCAAATGAACGGCGCCTCATACTTAATGGGTTCCTTGGTGATCAAAATAAAAATTTGAGTATGAAGTCAGCTCTGAAGCAAAAGAACAATGCCATCCCTGGAAAGGATGGTGGTTTGCCAGTTTCTGCTGATCAGTGGAAGACTGTTGAGTTGTCATCCAGCATATTGTCTGGCAAAAGTGGCAAGAGGGGCAAAATTGATGCTGATGTTGAAGAACTAAAGGAAGCAATCTCACTATTGTTACAACTGCCATATAGTTCCACCCCACCAACAGATACAGGACTTGAAGAGTCCCAAGGGAGTGTGAAGAGGCCTTTTGGGTCGATCTCTAACAAGATGGATCTTGGAGAAGGCACCCCTGGGTGTGAAGAATGCAGAAGAGCAAAGAGGCAAAAAGTGAGTGACGAAAGGAGCTCATATATCCTAGGAAATTCTCCAATTCCTTCAGATGATGAAGATACTTGGTGGATGAGGAAGAGACCTAAATCCTCAGAGCCGTTGAAGGTTGATCCACCAGTCAAGCTAACTAAACAGGTATCTAGGAATCGGCAGAAAATAGTTCGCAAAACTCAAAGTCTTGCTCAACTAGCAGCTGCTAGGATTGAAGGGAGCCAGGGAGCTTCTACAAGTCATGTCTGCAATAATAAGATAAGTTGTCCTCATCATAGATCTGGACTAGAGGGAGAACCTCCGAAGCCTACAGATACGACCAAAACGAACCATGCTGGAGATATTGTTTCTATTGGAAAAGCTTTAAAGCGGCTGCGATTTGCAGAGAAGAGGACTATTACTGTTTGGTTAATGACTAATATCAGGCAGCTTGTTGAAGAGACTGAAAAGACAATTGCTAAGGTTGGCCAGTTTGGTAGGAATTTCACAGCAGTTGATGATAGGAGCTCCATACGGTGGAAGCTCGGTGAGGATGAACTTTCTGCTGCACTGTATTTAATGGATGTTTCAGATGATTTAGTTTCAGCAGTAAAGTTTCTTCTTTGGCTGCTGCCAAAGGTTATTACTAGCCCCAGTTCTACTATCCATAGTGGGAGAAACATTCTGCTGCTGCCAAGAAATGTTGAAGGCCAAGTATGTGAAGTTGGGGAGGCATTTCTAATATCGTCCCTCCGAAGGTATGAGAACATACTTCTTGCAACAGATCTCATTCCAGAAGTCTTATCAGCAACAATGCTCCGTGCTTCAGCAGTGGTGGCATCTAACGGAAGAGTTTCAGGTTCAGCTGCCTTAGTTTACAGTCGGTATCTGTTGAAGCGGTATGGCAATGTGGCTAGTGTGATTGAATGGGAGAAGAGTTTCAAGGTAACATGTGATAAGAGGCTTTATTCTGAACTTGAATCTGGGCAAACAGTGGATGGAGAGTTAGGGTTTGCGCTTGGTGTTCCATCAGGAGTTGAGGATCTTGATGATTATTTTCGCCAAAAGATTAGTGGTGTCCGGTTATCTCGGGTGGGTTTGAACATGAGAGAGATTGTACAAAAAAATGTGAATGTTGACGATGCCTTTCCCTATTTTTCGGGCAAGGAGAGAAAACTCTTTGCCGGCAGCACTCCTAAAGCCCCTGCAATTGAAAAATGGGATGACGGGTATCAGATTGCTCATAAAATAATAACTGAACTGATGGACTGCATTCGGCAAACTGGTGGTGCTGCTCAAGAAGGCGACCCAACTTTGGTGTCATCCGCTGTTTCTGCTATTGTTGGTAATGTAGGGTCAATTCTAGCAAAAGTTTCTGATTTCAGACCAGGAGGTTATCCATCTGCCACTGATTCCCTAAACTTTGCTAGACGTATTCTACGCATCCATATAAGTTGCCTATGCCTTCTCAAGGAAGCTCTTGGAGAGCGTCAAACTCGTGTATTTGAGGTAGCTCTTGCCACAGAAGCTTGTTCTGCTCTTGCTGGGGTTTTTTCTCCTGGAAAGGGTTCTCGGAATCAATATCAGTTATCTCCTGAATCCCATGATTCCAATACAAAAGTTGCAGCTGCTGTTTCTGCTCTAATCATCGGGGCAGTTATTAATGGGATTACTAGCTTGGAGAGGTTGGTGACTGTGTTCAGGTTTAAGGAAAAGTTGGATATAATTCAATTTGTGAGGAATAGTAGATCCAACTCAAATGGTAATGCTCGTACTGCTGGGGCACTTAAGGGTGATAACTCACTTGAAGTTTATGTTCATTGGTTTAGATTGCTTGTTGGAAACTGCAGAACTGTTTCGGATGGAATGGTTGTGGAACTCTTGGGTGAACCATCCATAATAGCTCTTTCCAGGATGCAGCGAATGCTTCCTCTTCGTTTGGTTTTTCCACCTGCTTATTCAATATTTGCTTTTGTTATATGGTGGCCATTCCTTCTGAACACTTGCCTTGCAGTTCGTGAGGATTTTAATCAGTCATATCAATCTTTAGCAACAGCTATAGGTGATGTTATTAAGCATTTGCCTTTTCGTGATGTTTGTTTGAGAGATAGTCAGGGTTTCTATGACCTTGTAGCGGCAGATAGCAGCGATGCTGAATTTGCAGCCATGCTGGACTTGAACGGTTCAGACATTCATATTAAATCCATGGCTTTTGTTCCATTACGTGCAAGGCTTTTTCTGAATGCCATTCTTGACTGTAAGTTGCCAAACTCTTTATTTCCACAGGCGGAAGGAAATCAGGTATCTGGACAGGGCGAAACTAAAGTTCACTATTCAGAGCGTGAATCAAAGCTTGTCCATATTTTAGATACCCTTCAACCTGCAAAATTTCATTGGCAATGGGTAGAGCTCAGGCTTCTTTTGAATGAACAAGCCCTCATTGAAAAACTAGAGACACAGGACATGTCTTTAGTGGATGCCATACGTTCGTCCTCACCTAATCCAGAAAAGGCTGCTGCCTCTGAAAATGAGAAATATTTCATTGAAATCATCCTTACAAGATTATTGGTTAGACCTGATGCTGCACCCCTTTTCTCAGATGTGGTTCATCTCTTTGGGAGGTCTCTTGCAGATTCTATGTTGTTGCAGGTTAAATGGTTCCTGGGTGGTTCAGATGTTCTTTTTGGACGAAAAACCATTAGACAACGGCTTATGAATATTGCCGAGAGTAAAGGTCTCTCGACTAAGACCCACTTTTGGAAACCTTGGGGCTGGTTTACTTCTGAATCTGATCTTCTGACAAACAGGGGAGATAAGAAGAAGTTTGAAGTCACCTCCCTTGAAGAGGGTGAGATGGTTGAAGAGGGGACAGAGTCGAAAAGGCATGGGAAAGGGTCCAGTCAATTATTTGACAATGAAGGCCAGAGTGTCTCCCAGCAGCATGTGACAGAGAGGGCTCTCATTGAATTACTTCTTCCTTGCATAGATCAAAGTTCAGATGACTCACGAAATACTTTTGCAAATGATTTGATCAAGCAGTTGAGTAATATCGAGCAACAAATTAATGCTGTGACTCGCGGGACAAGCAAGCAAGCAGGACAAACTCCTTCTGGAATTGAAGGTCCCACAAGCAAAGGAAATAATCGCAAAGGTATAAGGGGTGGCAGTCCTGGATTAGCTAGACGTGCAGCAGCAGCAGCAGCGGCAGATTCTGCTCCACCGTCCCCTGCAGCTTTGCGTGCTTCTATGTCATTGAGATTGCAGTTACTCTTGAGATTGCTTCCAATCATCTATGCAGATAGGGAGCCATCAGCGCGGAGGCATGGGCTTGCCACCGTTATATTACGCCTTCTAGGAAACAGAGTTGTGCATGAGGATGCAGACCCATCCTTCACTCCCTCACAGAGTTCTTTATCCAAGAGGGAGTCAGATTCTTCAATGGATGCTGCCTCTGCTGCTCTTGCAGACTTGTCAAGCGAAAGTCTCTTTGATGAGTTATTGCTGGTTCTGCACGGGCTGTTAAGCAGTTGCCAGCCAAGTTGGTTGAGGTCTACTAAGCCAACAAAAGAAAGTGGGAAAGGTTTTGTTGCATTTGATCGTGAACTGGCAGAGAATTTGCAGAATGACTTGGATCGCATGCAGCTGCCTGACACTGTTAGGTGGCGTATTCAAACTGCAATGCCGGTAATAATTCCTTCAATTCGGTGCGTTGTGTCGTGCCAGCCACCACCAGTTCCAAACATGGCTTTGGCTGTTCTTCAGTCCAGCACATCAAATTCTGGGATTTACTCGGGAAACTCAAACCCACTGCAGAAAAATCAGGTTCCTCTGGCACGAACAGTAAGTAGTGTAACGGGGAAGTCGAAGCCATTGCCATCCCAGGATTATGATATGGAGATTGACCCATGGACACTCCTGGAAGATGGTGCAGGATCAGGCCCGAGCTCAGGCAACAGTGCTTTAATAGGCAGTGCAGATCATGGAAATCTTCGAGCATCTAGCTGGCTTAAGGGGGCTGTAAGAGTAAGACGGAAGGATCTCACATACATCGGTGCTGTGGACGATGACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006352 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016592 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:0070013 GO:0071704 GO:0090213 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med12

Protein Analysis

2248

Amino Acids

249.03

Weight (kDa)

8.85

Isoelectric Point (pI)

50.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med12	PF09497	159 - 219	3.3e-20	Transcription mediator complex subunit Med12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00450 AT4G00450 AT4G00450
fragaria_vesca	FvH4_7g14270 FvH4_7g14270 FvH4_7g14270
malus_domestica	MD01G1060600.v1.1 MD07G1137100.v1.1
prunus_persica	Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1
pyrus_communis	pycom01g09080 pycom07g13490
rosa_chinensis	RchiOBHm_Chr1g0355261
rosa_laevigata	RLG00000028166
rosa_multiflora	Rmu_sc0003115.1_g000044
rosa_roxburghii	Rroxscaffold_4G00300550
rosa_rugosa	Rorug01G0245800 Rorug01G0245900 Rorug01G0246000 Rorug01G0246100 Rorug01G0246200 Rorug01G0246200 Rorug01G0246200
rosa_samantha	Rh1AG258300 Rh1BG227900 Rh1CG242000 Rh1DG255700
rosa_wichuraiana	Rw1G022800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1604
AarI	CACCTGC	1 cut(s)	4633
Acc16I	TGCGCA	2 cut(s)	1112, 1545
Acc36I	ACCTGC	3 cut(s)	4633, 5062, 5311
AccB1I	GGYRCC	3 cut(s)	129, 2316, 2657
AccB7I	CCANNNNNTGG	1 cut(s)	3210
AccI	GTMKAC	2 cut(s)	1196, 6224
AccII	CGCG	3 cut(s)	1146, 5751, 5989
AciI	CCGC	8 cut(s)	3064, 3571, 3999, 4808, 4961, 5751, 5876, 5989
AcoI	YGGCCR	3 cut(s)	680, 3160, 4658
AcuI	CTGAAG	7 cut(s)	334, 990, 2289, 2399, 2733, 3483, 6416
AcyI	GRCGYC	2 cut(s)	130, 2317
AdeI	CACNNNGTG	1 cut(s)	1289
AfaI	GTAC	8 cut(s)	776, 1245, 1521, 1666, 1794, 1969, 3792, 4447
AflII	CTTAAG	2 cut(s)	4460, 6680
AflIII	ACRYGT	2 cut(s)	3623, 5136
AjiI	CACGTC	1 cut(s)	5858
AleI	CACNNNNGTG	1 cut(s)	495
AloI	GAACNNNNNNTCC	2 cut(s)	1732, 1764
Alw21I	GWGCWC	7 cut(s)	406, 744, 1959, 2720, 3203, 5085, 6620
Alw44I	GTGCAC	1 cut(s)	1955
AlwNI	CAGNNNCTG	5 cut(s)	1205, 2177, 2429, 4304, 5885
Ama87I	CYCGRG	4 cut(s)	1996, 3762, 6463, 6613
ApaI	GGGCCC	1 cut(s)	2003
ApaLI	GTGCAC	1 cut(s)	1955
AseI	ATTAAT	3 cut(s)	1893, 4335, 5736
Asp700I	GAANNNNTTC	9 cut(s)	73, 805, 1005, 3526, 3611, 4240, 4528, 5210, 5643
AspA2I	CCTAGG	2 cut(s)	2225, 2728
AspLEI	GCGC	7 cut(s)	132, 1113, 1546, 2070, 2319, 3691, 5989
AsuC2I	CCSGG	2 cut(s)	1997, 1998
AsuII	TTCGAA	1 cut(s)	1738
AvaI	CYCGRG	4 cut(s)	1996, 3762, 6463, 6613
AvaII	GGWCC	5 cut(s)	686, 747, 2168, 5568, 5795
AvrII	CCTAGG	2 cut(s)	2225, 2728
BaeGI	GKGCMC	3 cut(s)	1959, 2003, 4459
BalI	TGGCCA	2 cut(s)	3162, 4660
BamHI	GGATCC	1 cut(s)	1163
BanI	GGYRCC	3 cut(s)	129, 2316, 2657
BanII	GRGCYC	7 cut(s)	1997, 2003, 2720, 3203, 5085, 5632, 6620
BarI	GAAGNNNNNNTAC	4 cut(s)	1180, 1212, 3276, 3308
BauI	CACGAG	2 cut(s)	399, 4170
BbsI	GAAGAC	2 cut(s)	340, 2450
Bbv12I	GWGCWC	7 cut(s)	406, 744, 1959, 2720, 3203, 5085, 6620
BceAI	ACGGC	3 cut(s)	2330, 2785, 5396
BcgI	CGANNNNNNTGC	6 cut(s)	1102, 1136, 1936, 1970, 6526, 6560
BciVI	GTATCC	2 cut(s)	260, 702
BclI	TGATCA	6 cut(s)	565, 1429, 2032, 2344, 2434, 5703
BcnI	CCSGG	2 cut(s)	1997, 1998
BfmI	CTRYAG	7 cut(s)	1019, 1322, 3003, 4519, 4731, 5901, 6482
BfoI	RGCGCY	3 cut(s)	133, 2071, 2320
BfrI	CTTAAG	2 cut(s)	4460, 6680
BfuAI	ACCTGC	3 cut(s)	4633, 5062, 5311
BfuI	GTATCC	2 cut(s)	260, 702
BglII	AGATCT	4 cut(s)	593, 2208, 2972, 3457
BlnI	CCTAGG	2 cut(s)	2225, 2728
Bme18I	GGWCC	5 cut(s)	686, 747, 2168, 5568, 5795
BmeT110I	CYCGRG	4 cut(s)	1996, 3762, 6463, 6613
BmgBI	CACGTC	1 cut(s)	5858
BmrI	ACTGGG	1 cut(s)	3321
BmuI	ACTGGG	1 cut(s)	3321
BoxI	GACNNNNGTC	2 cut(s)	1088, 1130
BpiI	GAAGAC	2 cut(s)	340, 2450
BpmI	CTGGAG	2 cut(s)	1168, 3051
Bpu10I	CCTNAGC	3 cut(s)	3153, 5084, 6619
Bpu14I	TTCGAA	1 cut(s)	1738
BpuEI	CTTGAG	6 cut(s)	1058, 1140, 1660, 3954, 4127, 5965
BpuMI	CCSGG	2 cut(s)	1997, 1998
BsaAI	YACGTR	1 cut(s)	4898
BsaBI	GATNNNNATC	3 cut(s)	207, 1317, 4701
BsaHI	GRCGYC	2 cut(s)	130, 2317
BsaI	GGTCTC	3 cut(s)	2776, 5301, 5414
BsaWI	WCCGGW	1 cut(s)	3753
BsaXI	ACNNNNNCTCC	4 cut(s)	1732, 1762, 3024, 3054
Bse118I	RCCGGY	3 cut(s)	20, 3857, 6355
Bse3DI	GCAATG	7 cut(s)	629, 962, 1797, 3586, 5078, 6357, 6542
Bse8I	GATNNNNATC	3 cut(s)	207, 1317, 4701
BseJI	GATNNNNATC	3 cut(s)	207, 1317, 4701
BseMI	GCAATG	7 cut(s)	629, 962, 1797, 3586, 5078, 6357, 6542
BseMII	CTCAG	9 cut(s)	626, 737, 1326, 1421, 1802, 2808, 5098, 5283, 6633
BseRI	GAGGAG	3 cut(s)	248, 308, 1737
BseSI	GKGCMC	3 cut(s)	1959, 2003, 4459
BseYI	CCCAGC	4 cut(s)	857, 4217, 4451, 5607
BsgI	GTGCAG	8 cut(s)	54, 1067, 3225, 5241, 5880, 6167, 6621, 6666
Bsh1236I	CGCG	3 cut(s)	1146, 5751, 5989
BshNI	GGYRCC	3 cut(s)	129, 2316, 2657
BsiHKAI	GWGCWC	7 cut(s)	406, 744, 1959, 2720, 3203, 5085, 6620
BsiHKCI	CYCGRG	4 cut(s)	1996, 3762, 6463, 6613
BsiSI	CCGG	6 cut(s)	21, 679, 1997, 3754, 3858, 6356
BslFI	GGGAC	7 cut(s)	1138, 2581, 3406, 5554, 5767, 5781, 5881
BsmFI	GGGAC	7 cut(s)	1138, 2581, 3406, 5554, 5767, 5781, 5881
BsmI	GAATGC	5 cut(s)	187, 2681, 3945, 4602, 4921
Bso31I	GGTCTC	3 cut(s)	2776, 5301, 5414
BsoBI	CYCGRG	4 cut(s)	1996, 3762, 6463, 6613
Bsp119I	TTCGAA	1 cut(s)	1738
Bsp120I	GGGCCC	1 cut(s)	1999
Bsp1407I	TGTACA	2 cut(s)	1519, 3790
Bsp19I	CCATGG	3 cut(s)	1914, 4878, 6578
BspACI	CCGC	8 cut(s)	3064, 3571, 3999, 4808, 4961, 5751, 5876, 5989
BspCNI	CTCAG	9 cut(s)	627, 736, 1327, 1420, 1801, 2807, 5097, 5282, 6632
BspFNI	CGCG	3 cut(s)	1146, 5751, 5989
BspMAI	CTGCAG	4 cut(s)	1023, 4523, 5905, 6486
BspMI	ACCTGC	3 cut(s)	4633, 5062, 5311
BspQI	GCTCTTC	1 cut(s)	2677
BspT104I	TTCGAA	1 cut(s)	1738
BspT107I	GGYRCC	3 cut(s)	129, 2316, 2657
BspTI	CTTAAG	2 cut(s)	4460, 6680
BspTNI	GGTCTC	3 cut(s)	2776, 5301, 5414
BsrDI	GCAATG	7 cut(s)	629, 962, 1797, 3586, 5078, 6357, 6542
BsrFI	RCCGGY	3 cut(s)	20, 3857, 6355
BsrGI	TGTACA	2 cut(s)	1519, 3790
BssAI	RCCGGY	3 cut(s)	20, 3857, 6355
BssNAI	GTATAC	1 cut(s)	1197
BssNI	GRCGYC	2 cut(s)	130, 2317
BssSI	CACGAG	2 cut(s)	399, 4170
BssT1I	CCWWGG	9 cut(s)	1419, 1914, 2225, 2338, 2599, 2728, 4878, 5438, 6578
Bst1107I	GTATAC	1 cut(s)	1197
Bst2BI	CACGAG	2 cut(s)	399, 4170
BstACI	GRCGYC	2 cut(s)	130, 2317
BstAFI	CTTAAG	2 cut(s)	4460, 6680
BstAPI	GCANNNNNTGC	4 cut(s)	1373, 2264, 4597, 5885
BstAUI	TGTACA	2 cut(s)	1519, 3790
BstBAI	YACGTR	1 cut(s)	4898
BstBI	TTCGAA	1 cut(s)	1738
BstDSI	CCRYGG	3 cut(s)	1914, 4878, 6578
BstENI	CCTNNNNNAGG	4 cut(s)	1155, 1872, 4142, 5516
BstFNI	CGCG	3 cut(s)	1146, 5751, 5989
BstH2I	RGCGCY	3 cut(s)	133, 2071, 2320
BstHHI	GCGC	7 cut(s)	132, 1113, 1546, 2070, 2319, 3691, 5989
BstNSI	RCATGY	4 cut(s)	3627, 5140, 5618, 6313
BstPAI	GACNNNNGTC	2 cut(s)	1088, 1130
BstSFI	CTRYAG	7 cut(s)	1019, 1322, 3003, 4519, 4731, 5901, 6482
BstSLI	GKGCMC	3 cut(s)	1959, 2003, 4459
BstUI	CGCG	3 cut(s)	1146, 5751, 5989
BstV2I	GAAGAC	2 cut(s)	340, 2450
BstZ17I	GTATAC	1 cut(s)	1197
BsuI	GTATCC	2 cut(s)	260, 702
BtgI	CCRYGG	3 cut(s)	1914, 4878, 6578
BtgZI	GCGATG	1 cut(s)	4836
BtrI	CACGTC	1 cut(s)	5858
BtsI	GCAGTG	4 cut(s)	2265, 3510, 6479, 6649
BveI	ACCTGC	3 cut(s)	4633, 5062, 5311
CaiI	CAGNNNCTG	5 cut(s)	1205, 2177, 2429, 4304, 5885
CfoI	GCGC	7 cut(s)	132, 1113, 1546, 2070, 2319, 3691, 5989
Cfr10I	RCCGGY	3 cut(s)	20, 3857, 6355
Cfr9I	CCCGGG	1 cut(s)	1996
CseI	GACGC	1 cut(s)	4151
Csp6I	GTAC	8 cut(s)	775, 1244, 1520, 1665, 1793, 1968, 3791, 4446
CviQI	GTAC	8 cut(s)	775, 1244, 1520, 1665, 1793, 1968, 3791, 4446
DinI	GGCGCC	2 cut(s)	131, 2318
DraI	TTTAAA	2 cut(s)	120, 3060
DraIII	CACNNNGTG	1 cut(s)	1289
EaeI	YGGCCR	3 cut(s)	680, 3160, 4658
EciI	GGCGGA	1 cut(s)	4976
Ecl136II	GAGCTC	4 cut(s)	2718, 3201, 5083, 6618
Eco130I	CCWWGG	9 cut(s)	1419, 1914, 2225, 2338, 2599, 2728, 4878, 5438, 6578
Eco147I	AGGCCT	1 cut(s)	2617
Eco24I	GRGCYC	7 cut(s)	1997, 2003, 2720, 3203, 5085, 5632, 6620
Eco31I	GGTCTC	3 cut(s)	2776, 5301, 5414
Eco32I	GATATC	3 cut(s)	309, 757, 2014
Eco47I	GGWCC	5 cut(s)	686, 747, 2168, 5568, 5795
Eco53kI	GAGCTC	4 cut(s)	2718, 3201, 5083, 6618
Eco57I	CTGAAG	7 cut(s)	334, 990, 2289, 2399, 2733, 3483, 6416
Eco88I	CYCGRG	4 cut(s)	1996, 3762, 6463, 6613
EcoICRI	GAGCTC	4 cut(s)	2718, 3201, 5083, 6618
EcoNI	CCTNNNNNAGG	4 cut(s)	1155, 1872, 4142, 5516
EcoO109I	RGGNCCY	4 cut(s)	686, 1525, 2000, 5795
EcoRI	GAATTC	1 cut(s)	788
EcoRV	GATATC	3 cut(s)	309, 757, 2014
EcoT14I	CCWWGG	9 cut(s)	1419, 1914, 2225, 2338, 2599, 2728, 4878, 5438, 6578
EcoT38I	GRGCYC	7 cut(s)	1997, 2003, 2720, 3203, 5085, 5632, 6620
EgeI	GGCGCC	2 cut(s)	131, 2318
EheI	GGCGCC	2 cut(s)	131, 2318
ErhI	CCWWGG	9 cut(s)	1419, 1914, 2225, 2338, 2599, 2728, 4878, 5438, 6578
FalI	AAGNNNNNCTT	6 cut(s)	963, 995, 1381, 1413, 5411, 5443
FaqI	GGGAC	7 cut(s)	1138, 2581, 3406, 5554, 5767, 5781, 5881
FauI	CCCGC	1 cut(s)	5744
FbaI	TGATCA	6 cut(s)	565, 1429, 2032, 2344, 2434, 5703
FblI	GTMKAC	2 cut(s)	1196, 6224
FriOI	GRGCYC	7 cut(s)	1997, 2003, 2720, 3203, 5085, 5632, 6620
FspI	TGCGCA	2 cut(s)	1112, 1545
GlaI	GCGC	7 cut(s)	131, 1112, 1545, 2069, 2318, 3690, 5988
GsaI	CCCAGC	4 cut(s)	861, 4221, 4455, 5611
GsuI	CTGGAG	2 cut(s)	1168, 3051
HaeII	RGCGCY	3 cut(s)	133, 2071, 2320
HapII	CCGG	6 cut(s)	21, 679, 1997, 3754, 3858, 6356
HgaI	GACGC	1 cut(s)	4151
HhaI	GCGC	7 cut(s)	132, 1113, 1546, 2070, 2319, 3691, 5989
Hin1I	GRCGYC	2 cut(s)	130, 2317
Hin6I	GCGC	7 cut(s)	130, 1111, 1544, 2068, 2317, 3689, 5987
HinP1I	GCGC	7 cut(s)	130, 1111, 1544, 2068, 2317, 3689, 5987
HincII	GTYRAC	2 cut(s)	1016, 3811
HindII	GTYRAC	2 cut(s)	1016, 3811
HindIII	AAGCTT	4 cut(s)	1395, 3054, 4200, 5024
HpaII	CCGG	6 cut(s)	21, 679, 1997, 3754, 3858, 6356
HpyCH4IV	ACGT	7 cut(s)	376, 1012, 2098, 4105, 4897, 5158, 5857
HpySE526I	ACGT	7 cut(s)	376, 1012, 2098, 4105, 4897, 5158, 5857
Hsp92I	GRCGYC	2 cut(s)	130, 2317
HspAI	GCGC	7 cut(s)	130, 1111, 1544, 2068, 2317, 3689, 5987
KasI	GGCGCC	2 cut(s)	129, 2316
KroI	GCCGGC	1 cut(s)	3857
KroNI	GCCGGC	1 cut(s)	3859
Ksp22I	TGATCA	6 cut(s)	565, 1429, 2032, 2344, 2434, 5703
LguI	GCTCTTC	1 cut(s)	2677
LmnI	GCTCC	9 cut(s)	1750, 2715, 2913, 2921, 3198, 3206, 3495, 5893, 5977
MaeII	ACGT	7 cut(s)	376, 1012, 2098, 4105, 4897, 5158, 5857
MfeI	CAATTG	2 cut(s)	3147, 3882
MlsI	TGGCCA	2 cut(s)	3162, 4660
MluNI	TGGCCA	2 cut(s)	3162, 4660
Mly113I	GGCGCC	2 cut(s)	130, 2317
MlyI	GAGTC	7 cut(s)	942, 1244, 2603, 5555, 5669, 5740, 6107
MmeI	TCCRAC	4 cut(s)	2006, 4371, 4449, 4492
Mox20I	TGGCCA	2 cut(s)	3162, 4660
MroNI	GCCGGC	1 cut(s)	3857
MroXI	GAANNNNTTC	9 cut(s)	73, 805, 1005, 3526, 3611, 4240, 4528, 5210, 5643
MscI	TGGCCA	2 cut(s)	3162, 4660
MslI	CAYNNNNRTG	5 cut(s)	495, 1654, 1857, 2124, 6048
Msp20I	TGGCCA	2 cut(s)	3162, 4660
MspA1I	CMGCKG	9 cut(s)	1130, 2137, 2897, 3539, 4001, 4301, 5876, 6316, 6744
MspCI	CTTAAG	2 cut(s)	4460, 6680
MspI	CCGG	6 cut(s)	21, 679, 1997, 3754, 3858, 6356
MunI	CAATTG	2 cut(s)	3147, 3882
Mva1269I	GAATGC	5 cut(s)	187, 2681, 3945, 4602, 4921
MvnI	CGCG	3 cut(s)	1146, 5751, 5989
NaeI	GCCGGC	1 cut(s)	3859
NarI	GGCGCC	2 cut(s)	130, 2317
NciI	CCSGG	2 cut(s)	1997, 1998
NcoI	CCATGG	3 cut(s)	1914, 4878, 6578
NgoMIV	GCCGGC	1 cut(s)	3857
NmeAIII	GCCGAG	2 cut(s)	1786, 5422
NmuCI	GTSAC	9 cut(s)	497, 658, 905, 2173, 2705, 4363, 5503, 5617, 5743
NsbI	TGCGCA	2 cut(s)	1112, 1545
NspI	RCATGY	4 cut(s)	3627, 5140, 5618, 6313
NspV	TTCGAA	1 cut(s)	1738
OliI	CACNNNNGTG	1 cut(s)	495
PaeI	GCATGC	1 cut(s)	6313
PaqCI	CACCTGC	1 cut(s)	4633
PasI	CCCWGGG	1 cut(s)	2663
PceI	AGGCCT	1 cut(s)	2617
PciI	ACATGT	2 cut(s)	3623, 5136
PciSI	GCTCTTC	1 cut(s)	2677
PcsI	WCGNNNNNNNCGW	2 cut(s)	3017, 6533
PctI	GAATGC	5 cut(s)	187, 2681, 3945, 4602, 4921
PdiI	GCCGGC	1 cut(s)	3859
PdmI	GAANNNNTTC	9 cut(s)	73, 805, 1005, 3526, 3611, 4240, 4528, 5210, 5643
PflFI	GACNNNGTC	1 cut(s)	5545
PflMI	CCANNNNNTGG	1 cut(s)	3210
PfoI	TCCNGGA	4 cut(s)	4231, 4583, 5842, 6588
PleI	GAGTC	7 cut(s)	942, 1244, 2602, 5554, 5669, 5740, 6106
PluTI	GGCGCC	2 cut(s)	133, 2320
PpsI	GAGTC	7 cut(s)	942, 1244, 2602, 5554, 5669, 5740, 6106
Ppu21I	YACGTR	1 cut(s)	4898
PpuMI	RGGWCCY	2 cut(s)	686, 5795
PscI	ACATGT	2 cut(s)	3623, 5136
PshAI	GACNNNNGTC	2 cut(s)	1088, 1130
PshBI	ATTAAT	3 cut(s)	1893, 4335, 5736
PsiI	TTATAA	1 cut(s)	1604
Psp124BI	GAGCTC	4 cut(s)	2720, 3203, 5085, 6620
Psp5II	RGGWCCY	2 cut(s)	686, 5795
PspFI	CCCAGC	4 cut(s)	857, 4217, 4451, 5607
PspOMI	GGGCCC	1 cut(s)	1999
PspPPI	RGGWCCY	2 cut(s)	686, 5795
PstI	CTGCAG	4 cut(s)	1023, 4523, 5905, 6486
PstNI	CAGNNNCTG	5 cut(s)	1205, 2177, 2429, 4304, 5885
PsyI	GACNNNGTC	1 cut(s)	5545
PvuII	CAGCTG	7 cut(s)	1130, 2137, 2897, 3539, 4301, 6316, 6744
RsaI	GTAC	8 cut(s)	776, 1245, 1521, 1666, 1794, 1969, 3792, 4447
RsaNI	GTAC	8 cut(s)	775, 1244, 1520, 1665, 1793, 1968, 3791, 4446
RseI	CAYNNNNRTG	5 cut(s)	495, 1654, 1857, 2124, 6048
SacI	GAGCTC	4 cut(s)	2720, 3203, 5085, 6620
SapI	GCTCTTC	1 cut(s)	2677
SchI	GAGTC	7 cut(s)	942, 1244, 2603, 5555, 5669, 5740, 6107
SfcI	CTRYAG	7 cut(s)	1019, 1322, 3003, 4519, 4731, 5901, 6482
SfoI	GGCGCC	2 cut(s)	131, 2318
SfuI	TTCGAA	1 cut(s)	1738
SinI	GGWCC	5 cut(s)	686, 747, 2168, 5568, 5795
SmaI	CCCGGG	1 cut(s)	1998
SmiMI	CAYNNNNRTG	5 cut(s)	495, 1654, 1857, 2124, 6048
SmlI	CTYRAG	8 cut(s)	1037, 1155, 1639, 3969, 4142, 4460, 5944, 6680
SmoI	CTYRAG	8 cut(s)	1037, 1155, 1639, 3969, 4142, 4460, 5944, 6680
SphI	GCATGC	1 cut(s)	6313
SrfI	GCCCGGGC	1 cut(s)	1998
SseBI	AGGCCT	1 cut(s)	2617
SsiI	CCGC	8 cut(s)	3064, 3571, 3999, 4808, 4961, 5751, 5876, 5989
SspDI	GGCGCC	2 cut(s)	129, 2316
SspI	AATATT	4 cut(s)	1897, 4638, 5210, 5392
SstI	GAGCTC	4 cut(s)	2720, 3203, 5085, 6620
StuI	AGGCCT	1 cut(s)	2617
StyI	CCWWGG	9 cut(s)	1419, 1914, 2225, 2338, 2599, 2728, 4878, 5438, 6578
TaiI	ACGT	7 cut(s)	379, 1015, 2101, 4108, 4900, 5161, 5860
TatI	WGTACW	3 cut(s)	1519, 1967, 3790
TauI	GCSGC	3 cut(s)	3067, 4811, 5879
TseFI	GTSAC	9 cut(s)	497, 658, 905, 2173, 2705, 4363, 5503, 5617, 5743
Tsp45I	GTSAC	9 cut(s)	497, 658, 905, 2173, 2705, 4363, 5503, 5617, 5743
TspGWI	ACGGA	5 cut(s)	889, 1124, 3482, 3534, 6718
TspMI	CCCGGG	1 cut(s)	1996
Tth111I	GACNNNGTC	1 cut(s)	5545
Van91I	CCANNNNNTGG	1 cut(s)	3210
Vha464I	CTTAAG	2 cut(s)	4460, 6680
VneI	GTGCAC	1 cut(s)	1955
VpaK11BI	GGWCC	5 cut(s)	686, 747, 2168, 5568, 5795
VspI	ATTAAT	3 cut(s)	1893, 4335, 5736
XagI	CCTNNNNNAGG	4 cut(s)	1155, 1872, 4142, 5516
XceI	RCATGY	4 cut(s)	3627, 5140, 5618, 6313
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3743
XmaI	CCCGGG	1 cut(s)	1996
XmaJI	CCTAGG	2 cut(s)	2225, 2728
XmiI	GTMKAC	2 cut(s)	1196, 6224
XmnI	GAANNNNTTC	9 cut(s)	73, 805, 1005, 3526, 3611, 4240, 4528, 5210, 5643

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.