Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002990

Physical Location & Seq

Genomic Coordinates Reverse (-)

16413355 .. 16427226

Gene Structure 13872 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1060600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6804 bp

ATGAGCAGGTATCATGCCACTGGCTGCACTAGTGCAGTTAACAACACAGCCATAGGTGGGACATCGGGGAGGGATTCTACCCGAGCTGAATCGGCTGCATCTCCGAACTTGCCTCTAGCTTCTAGGCGCTCTTCACAGTTAAACCCTTACAAGTTGAAGTGTGATAAAGACGATTTGAATTCCCGACTTGGGCCACCTGACTTTCACCCCCAAAAACCAAATTGCCCAGAGGAAACTCTTACCGAAGAATATGTGCAAGCTGGATACAGAGAGACTGTTGAAGGAATTGAGGAATCAAGAGAGGTTTCTCTAAGTCAGGCTCCAACTTTTGGCAAGCCCCTTGTCTTCAGGTGCAAAGAGGCCATCAAAATACGTTTTAGGGCAATTAATGAATCTCGTGCTCAAAAGAGAAAGGCTGGTCAAGTGTACGGTGTTCCTCTTGTTGATTCACTGTTGTCAAAGCCAGGTGTTTTTCCTGAACAAAAGCCCTGTGGTGAAGGGTTGCGAAAAAAATGGATTGAGGGTCTATCACAACAACACAAGCGCTTGCGTTCATTGGCTGATCATGTCCCTCATGGATATAGGAAAAGATATTTATTTGAAGTTCTTACAAGGAATAATGTTCCATTGCTGAGAGCAACTTGGTTTATTAAAGTAACTTATCTTAATCAGGTCAGGCCAGGTTCTACCAATATATCTTCTGGGACATCTGATAAAGCTCAGTTGTCCCGCACAGAGCTGTGGACAAAAGATATTATTGATTACCTGCAGTACCTTCTCGATGAGTTATTCTCAAGAAATAGTTCTCACTCCACTTCACTCAACAGAGATAGATCGCCACAAATTCTCTATGCTGGGTCACTGCCACAAAGGGGTGATCCAGCATCAGCAGTCCTTGATGGTGAGGAGCCTTCTATACATTTCAAATGGTGGTATGTGGTGCGGCTTCTGCAATGGCATCATGCTGAGGGACTGCTTCTTCCTACACTCATCATTGAGTGGGTTCTAAGTCAACTACAGGAGAAAGAATTGCTTGAGATTATGCAGTTGCTTTTGCCTATTATATACGGTGTTCTGGAAACTATTGTTTTATCTCAAACATATGTGCGCAATCTTGTGAGAGTAGCTGTACGTTTCATCGGCGAACCTGCTCAAGGTGGATCAGATCTTGTAGATAACTCCCATAGGGGGTATACAGTGTCTTCTTTGGTTGAGATGCTTCGCTATTTAATAATTGCTGTGCCTGATACCTTTGTAGCTTTTGATTGCTTTCCTCTACCATCCTCTGTAGTCTCCTATGTTGCAAATGATGGATTACCAAAGATGTCTGATGCGAGAAAGATTAAAAATGTTTCAGCAGAAGTTGCTTCTGCATTCAGAAGTAAAGTTTTTGATACTCAATATCAGTCCCTGGCTTTTGATCATATTGTTTCATCCATTCAGAAACATGCTGAAAATCTTGCTAAGGCTGCAAGACCTAGCTATCCAGGCCATAGTATAGCTAAAGCTGTACAGGCCCTAGATGGATCTCTTGTGCAGGGAGATGTTCGAGCGGCTTATAGATTTCTGTTTGAAGATCCTTGTGATGGAGTTGCAAATGAAAGTTGGATTGCAGGAGTTAGTCCATGTTTGCGAACTTCGTTGAAGTGGATTGGGACTGCAAACTTGTCATGTGTTTGCTCTGTATTTTTCCTCTGTGAGTGGGCAACATGTGATTTCAGGGATTTTAGGACTGCCCCACCTTGTGAGCTGAAATTTACTGGCAGAAAGGATTTCTCCCAGGTATTTGTTGTTACTCGGCTTTTGAAGCTAAAGATGAGAGATTTGCAACTTTCACCTCAACGTAAGAATGATAGCATCCCTGGAGTCAGTAGTGTTGCAAAAGGTTCTACCCAGCAGAACAATTTTCCTGTCACAGTTTCCATGGGAAATTCCTGTGAAATTAAACCAAAAAATGTTGATCAAAGAAGTATGAAATCATCAAATATATTTGAGAGCCCTGGTCCTTTACATGATATCATAGTGTGTTGGATTGATCAACATGAAGCAGGCAAAGGAGAAGGTTTCAAGCGCCTTCAACTACTTGTAATCGAACTTATCCGGTCTGGTATTTTTAATCCTCATGCCTATGTTAGGCAACTGATAGTTAGTGGAATTATGGATACCAATGGAACGGGGGTTGAAGTCGACCGTTGGAATAGGCATTTTCGTATCTTGAAGCTGTTGCCTGCGCACTTTATGCACGATGCTTTGGAAGAAGCAGGGATTGCTGAAGGGCCACAACTTTCAGAGGCCATGAACTTCTACTCAACTGAGCGCCGCCTAATACTTCGTGGGCTTCTCTCCAATCAAAATAAAAATGTGAATATGAATATGTCTGTTCTGAAGCAAAAGCATTATTCCATTCCTGGAAAAGATGGTGGTTTGCCAGTTTCTGTGGATCGGTGGAAGGCTGTTCAGCCATCACCCAATGTATTGTCTGGTAAAAGTAGTAAGACTGATGTTGATGTCGAAGAACTAAAGGAAGCAATATCAGTTCTGTTGCATCTCCCAAGTAGTTCATCCCCAACGACAGAAACAGGGTTTGATGAATCCCAAGGAAGTGTTAAGAGGCCGTTTGGGTCAACCTTTAACAAGATGGATCTGGGAGAAGGCACCCCTGGGTGTGAAGAATGCAGAAGAGCAAAGAGGCAAAAAGTGAGTGACGAAAGGAGCTCATGCATCCAAGGAAATTCTCCAATTCCTTCAGATGATGAAGATGCCTGGTGGATGAGGAAGAGACCTAAGTCCTTGGAGCCATTGAAGGTTGATCCACCCGTTAAATCGACCAAACAGGTCTCTAGGAACCGGCAGAAGATAGTTCGTAAAACTCAAAGCCTTGCTCAACTTGCAGCTGCTAGGATTGAAGGTAGCCAGGGAGCATCTACGAGTCATGTCTGCAATAATAAAGTAAGCTGTCCTCACCATAAAGGTGGAGTTGAGGGGGAAACCCCGAAGTTTGTAGATCCAACCAAAATGAACCATGGTGGGGATATTGTTTCAATTGGAAAAGCTTTAAAGCGGCTGCGATTTGTAGAGAAGAGGACAATTACGGTTTGGTTGATGACTGTTATTAGGCAGCTTGTTGAAGAGACTGAAAAGACCATTGCGAAGGTTGGCCAATTTGGTAGGAATTTCACATCTGTTGATGATAGGAGCTCCATACGGTGGAAGCTTGGTGAAGATGAACTTTCTGCTGCACTGTATTTGATGGATGTTTCAAATGATTTAGTTTCAGCAGTCAAGTTTCTGCTTTGGTTGCTGCCAAAGGTTAATGGCCCCAGTTCTACAATCCATAGTGGGAGGAACATTATGCTGTTGCCAAGAAATGCTGAAAGTCAAGTGTGTGAAGTGGGGGAGGCATTTCTGGTATCATCACTCCGAAGGTATGAGAACATACTTATTGCTACAGATCTTATTCCAGAAGTCTTGTCAGTTACAATGCACCGTGCTTCAGCCATAGTGGCACCTAATGGAAGAGTTTCAGGTTCAGCTGCCTTAGCATACAGTCGGTATTTATTGAAGCGATACAGCAATGTAGCTAGTGTCATTGAATGGGAAAAGAATTTCAAGCCAACATGTGATAAGAGACTTCTTTCTGAACTTGAGTCTGGACAGTCAGTGGATGGAGAGTTGGGGTTTCCGCTTGGTGTTCCAGCAGGAGTCGAAGATCTTGATGATTTTTTCCGCCAAAAGATTAGCGGTGTTCGGTTATCCAGGGTGGGTTTAAACATGAAAGAAATAGTGCAACGAAATGTGAATGTTGATGATGCCCTTCAGTATTTTTTTGGGAAAGAGAGAAAGCTCTTTGCTACTGGTGCTCCTAAAGGCCCTCCTGTTGATAAATGGGATGACGGATGTCAGATATCTCAAAAAGTAATTACAGAACTGATGGACTGCATTAGGCAGACTGGTGGTGCTGCTCAAGAAGGGGATCCATCTTTGGTGTCTTCTGCCGTTTCTGCTATTGTCGGCAATGTAGGGCTAATTATAGCAAAGATTCCAGATTTTAGAATGGGGGGTAGCTATTCAACCTTTCCATCTGCCACTGATTCCTTGAACTTCGCTAGGCGTATTTTGCGTATCCATATAAGTTGCCTATGCCTCCTTAAGGAAGCTCTTGGAGAGCGCCAAACCCGTGTATTTGAGGTAGCTCTTGCGACAGAAGCTTGTTCTGCTCTTGCTCCTGGAAAGGCGTCTCGAAATCAATATCAATCCTCTCCTGAATCCCATGATTCCAACACTAACATGTCTAATGACATTTTGAACAGTAAAGTAGTTATTGGGAGGACAACAAAAGTTGCAGCTGCTGTTTCTGCACTAATCATTGGGGCGGTTGTTCAAGGGGTTACTAGCTTGGAGAGATTGGTGACTCTATTCAGGTTAAAGGAAAGGTTGGACGTAATTCAATTTGTAAGGAGTAGTAGATCCAACTCTAATGGCAATGCTCGTTCTGCTGGGGCATTTAAGGGTGATAACTTGCTTGAAGTTTATGTGCATTGGTTTAGACTTCTTGTTGGAAACTGCAGAACAGTCTCTGATGGATTGGTCGTGGAACTCTTGGGTGAACCCTCTGTAGTAGCTCTTTCAAGGATGCAACGTATGCTCCCTCTTGGTTTGGTTTTTCCACCTGCCTATTCTATATTTGCTTTTGTTATGTGGCGGCCATACCTTCTTAGCACTAGCTTTGCAGCGCGTGATGATTTTAACCAATCATATCAATCTTTAACAACAGCTATAGGTGATGCTATAAAGCACTCTCCTTTTCGAGATGTGTGTTTGAGAGATAGTCAGGGTTTCTATGATATTGTAGCTGCAGATGGCAGTGATGCTGAATTTGCAGCCATGCTAGAGTTAAATGGTTCAGACATGCATAAAAAATCCATGGCTTTTGTTCCATTACGTGCAAGGCTTTTCCTAAATGCCATAATGGATTGTAAGATGCCACACTCTTCATTTACACAGGGTGAAGCAAATCAGGTCTCTGGACATGGTGAGTCTAAAGTTAAATTTGCAGAGCGTGAAACAAAGCTTGTAGATAAGCTTGTACATATTCTAGATACCCTGCAACCTGCAAAATTTCATTGGCAGTGGGTAGAGCTTAGGCTCCTTTTGAATGAACAGGCCCTTATTGATAAACTTGAGACTCAAGATGCGTCTTTAGTGGATGCCATTCGGTCGTCCTCGCCTAGTCCTGAAAGGGCTGCTGCCTCCGAGAATGAGAAACATTTCATCGAGATCATCCTTACAAGGTTATTAGTTAGACCTGATGCTGCACCCCTTTTCTCGGATGTGGTTCATCTTTTTGGGAGGTCACTAGCAGATTCTATGTTGTTGCAGGTCAAATGGTTCCTGGGTGGCACAGATGTTCTTTATGGACGAAAAACAATTAGACAACGACTTCTTAATATTGCTGAGAGTAAAGGTCTCTCAACTAAGACCCAATTTTGGAAGCCGTGGGGATGGTGTATTTATGATGTTGATCCTGTAACAAACAGGGGAGATAAGAGAAAATTTGAAGTCACTTCCCTTGAAGAGGGTGAGATGGTTGAAGAGGGAACAGACTCAAAAAAGTATGGGAAAGGGGCCAGTCAAACGTTGGACATTGAAAGCTACAATGTCACTCAGCATTATGTGACAGAGAGGGCTCTCATTGAATTACTTCTTCCATGTATAGATCAAAGTTCTGATGAGTCGCGCAATACCTTTGCAAATGATTTGATCAAGCAGTTGATTAGTATTGAGCAACAAATTAGTGCAGGTACTCGTGGGACACATAAGCAAGCAGGACCAACTCCTTCTGGAGTTGAAGGTCCCACTGGCAAAGGAAATAGCCGCAAAGGTATAAGGGGTGGCAGTCCTGGATTAGCTAGGCGTGCAGCAGGAGCAGCTGATTATGCTCCACCGTCCCCTGCAGCTTTGCGAGCTTCTATGTCGCTGCGGTTGCATTTGCTCTTAAGATTGCTTCCTATCATATGGGCAGACAGGGAGCCATCTGCCCGGAACATGAGGCATGGATTTGCCTCTGTAGTACTACGTCTTCTAGGAAACCGAGTTGTGCATGAGGGTGCAGACCTATGCTTCAATATCATGCAGAGTACTTTCTCTAAGAGGGATGCAGAGTCTTCAAGAGAAGCTGCCTCTGCAGCTTTTGCAGAATTGTCAAATGAAAGTCTCTTTAATCAGTTATTGTTCGTTCTTCATGGGCTGTTAAGCAGTTACCGGCCAAGGTGGTTGAGGTCTACTAAGTCAACAAATGAGGGGGGAAAAGATTTTGCTGCATTTGATTGTGAACTGGCAGATATTTTGCAGAAAGACTTAGATCGAATGCAGCTTCCTGAAATGATTCGCTGGCGTATCCAAACTGCAATGCCAGTGGTTCTCCCTTCTGTTCGGTGCTTTTTCTCTTGCCAACCACCACCTGTTCCAGATACTGGTTTGGCTGTTTTTCAACCCAGCATATCGGTTTCTGGGTTGCATGCTGGAAACTCAAACCCACCTCAGAGAAATCAGGCTCCTCTTGCGCGAACAGTAACTAACATACCAGGGAAATTTAAACCATTGCCATCACAAGATTATGATATGGATATTGACCCATGGACGCTGCTGGAAGATGGTGCAGGATCAGGCCCATCTTCAAGTAGCAGTGCTTTGATAGGCAGTGCGGACCATGGCAATCTTCGAGCATCTAGCTGGCTCAAGGGGACTGTGAGGGTGAGACGAAAGGATCTCACATACATTGGTGCTGTGGATGATGACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006352 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016592 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:0070013 GO:0071704 GO:0090213 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med12

Protein Analysis

2268

Amino Acids

250.97

Weight (kDa)

8.87

Isoelectric Point (pI)

46.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med12	PF09497	158 - 218	7.3e-19	Transcription mediator complex subunit Med12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0002990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00450 AT4G00450 AT4G00450
fragaria_vesca	FvH4_7g14270 FvH4_7g14270 FvH4_7g14270
malus_domestica	MD01G1060600.v1.1 MD07G1137100.v1.1
prunus_persica	Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1
pyrus_communis	pycom01g09080 pycom07g13490
rosa_chinensis	RchiOBHm_Chr1g0355261
rosa_laevigata	RLG00000028166
rosa_multiflora	Rmu_sc0003115.1_g000044
rosa_roxburghii	Rroxscaffold_4G00300550
rosa_rugosa	Rorug01G0245800 Rorug01G0245900 Rorug01G0246000 Rorug01G0246100 Rorug01G0246200 Rorug01G0246200 Rorug01G0246200
rosa_samantha	Rh1AG258300 Rh1BG227900 Rh1CG242000 Rh1DG255700
rosa_wichuraiana	Rw1G022800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	4678
Acc16I	TGCGCA	2 cut(s)	1109, 2232
Acc36I	ACCTGC	6 cut(s)	774, 1156, 4678, 5119, 5368, 5789
AccB1I	GGYRCC	2 cut(s)	2654, 3505
AccB7I	CCANNNNNTGG	3 cut(s)	329, 3207, 6473
AccBSI	CCGCTC	1 cut(s)	1553
AccI	GTMKAC	3 cut(s)	1193, 2187, 6281
AccII	CGCG	3 cut(s)	4735, 5737, 6565
AclI	AACGTT	1 cut(s)	5636
AcoI	YGGCCR	3 cut(s)	3157, 4703, 6263
AcuI	CTGAAG	6 cut(s)	331, 2292, 2405, 2730, 3477, 3800
AcyI	GRCGYC	1 cut(s)	4235
AdeI	CACNNNGTG	2 cut(s)	1745, 5006
AfaI	GTAC	8 cut(s)	428, 773, 1131, 1512, 5088, 5803, 6072, 6139
AfeI	AGCGCT	1 cut(s)	545
AflII	CTTAAG	2 cut(s)	4148, 5995
AflIII	ACRYGT	3 cut(s)	1709, 3617, 4287
AhlI	ACTAGT	1 cut(s)	29
Alw21I	GWGCWC	4 cut(s)	403, 2717, 3200, 3862
AlwNI	CAGNNNCTG	5 cut(s)	1411, 2435, 4349, 4577, 6473
Ama87I	CYCGRG	1 cut(s)	81
Aor51HI	AGCGCT	1 cut(s)	545
AseI	ATTAAT	1 cut(s)	387
Asp700I	GAANNNNTTC	5 cut(s)	802, 3520, 5267, 5700, 6384
AsuC2I	CCSGG	1 cut(s)	6040
AvaI	CYCGRG	1 cut(s)	81
AvaII	GGWCC	4 cut(s)	2003, 5828, 5852, 6706
BaeI	ACNNNNGTAYC	2 cut(s)	1239, 1272
BalI	TGGCCA	1 cut(s)	3159
BamHI	GGATCC	1 cut(s)	3973
BanI	GGYRCC	2 cut(s)	2654, 3505
BanII	GRGCYC	4 cut(s)	2000, 2717, 3200, 5689
BarI	GAAGNNNNNNTAC	2 cut(s)	6063, 6095
BauI	CACGAG	2 cut(s)	396, 5805
BbsI	GAAGAC	5 cut(s)	337, 1194, 3981, 6071, 6156
Bbv12I	GWGCWC	4 cut(s)	403, 2717, 3200, 3862
BbvCI	CCTCAGC	1 cut(s)	966
BceAI	ACGGC	3 cut(s)	2599, 3980, 5479
BciVI	GTATCC	4 cut(s)	257, 2155, 4133, 6407
BclI	TGATCA	5 cut(s)	562, 1420, 1960, 2035, 5760
BcnI	CCSGG	1 cut(s)	6040
BcuI	ACTAGT	1 cut(s)	29
BfoI	RGCGCY	5 cut(s)	130, 547, 2074, 2320, 4171
BfrI	CTTAAG	2 cut(s)	4148, 5995
BfuAI	ACCTGC	6 cut(s)	774, 1156, 4678, 5119, 5368, 5789
BfuI	GTATCC	4 cut(s)	257, 2155, 4133, 6407
BglI	GCCNNNNNGGC	1 cut(s)	3503
BglII	AGATCT	3 cut(s)	1165, 3451, 3709
BmcAI	AGTACT	2 cut(s)	6072, 6139
Bme18I	GGWCC	4 cut(s)	2003, 5828, 5852, 6706
BmeT110I	CYCGRG	1 cut(s)	81
BmrI	ACTGGG	1 cut(s)	3315
BmuI	ACTGGG	1 cut(s)	3315
BoxI	GACNNNNGTC	2 cut(s)	2785, 3897
BpiI	GAAGAC	5 cut(s)	337, 1194, 3981, 6071, 6156
BplI	GAGNNNNNCTC	2 cut(s)	776, 808
BpmI	CTGGAG	2 cut(s)	1884, 5862
Bpu10I	CCTNAGC	4 cut(s)	966, 1464, 3538, 5141
BpuEI	CTTGAG	8 cut(s)	778, 1055, 1137, 3665, 3948, 5172, 5201, 6722
BpuMI	CCSGG	1 cut(s)	6040
BsaAI	YACGTR	1 cut(s)	4943
BsaBI	GATNNNNATC	1 cut(s)	5520
BsaHI	GRCGYC	1 cut(s)	4235
BsaI	GGTCTC	4 cut(s)	2773, 2842, 5026, 5471
BsaWI	WCCGGW	1 cut(s)	2100
BsaXI	ACNNNNNCTCC	4 cut(s)	3844, 3874, 4629, 4659
Bse118I	RCCGGY	2 cut(s)	2847, 6261
Bse3DI	GCAATG	8 cut(s)	626, 959, 3144, 3580, 4021, 4489, 6414, 6599
Bse8I	GATNNNNATC	1 cut(s)	5520
BseJI	GATNNNNATC	1 cut(s)	5520
BseMI	GCAATG	8 cut(s)	626, 959, 3144, 3580, 4021, 4489, 6414, 6599
BseMII	CTCAG	7 cut(s)	623, 734, 957, 2304, 5445, 5678, 6554
BseRI	GAGGAG	2 cut(s)	920, 6546
BseYI	CCCAGC	4 cut(s)	854, 1893, 4496, 6494
Bsh1236I	CGCG	3 cut(s)	4735, 5737, 6565
Bsh1285I	CGRYCG	2 cut(s)	2191, 5219
BshNI	GGYRCC	2 cut(s)	2654, 3505
BsiEI	CGRYCG	2 cut(s)	2191, 5219
BsiHKAI	GWGCWC	4 cut(s)	403, 2717, 3200, 3862
BsiHKCI	CYCGRG	1 cut(s)	81
BsiSI	CCGG	4 cut(s)	2101, 2848, 6040, 6262
BsmBI	CGTCTC	2 cut(s)	4242, 6751
BsmI	GAATGC	3 cut(s)	1373, 2678, 6372
Bso31I	GGTCTC	4 cut(s)	2773, 2842, 5026, 5471
BsoBI	CYCGRG	1 cut(s)	81
Bsp1286I	GDGCHC	6 cut(s)	403, 2000, 2717, 3200, 3862, 5689
Bsp1407I	TGTACA	2 cut(s)	1510, 5086
Bsp19I	CCATGG	5 cut(s)	1923, 3022, 4923, 6635, 6709
BspCNI	CTCAG	7 cut(s)	624, 733, 958, 2305, 5446, 5677, 6553
BspFNI	CGCG	3 cut(s)	4735, 5737, 6565
BspMAI	CTGCAG	5 cut(s)	771, 4568, 4858, 5956, 6187
BspMI	ACCTGC	6 cut(s)	774, 1156, 4678, 5119, 5368, 5789
BspQI	GCTCTTC	2 cut(s)	136, 2674
BspT107I	GGYRCC	2 cut(s)	2654, 3505
BspTI	CTTAAG	2 cut(s)	4148, 5995
BspTNI	GGTCTC	4 cut(s)	2773, 2842, 5026, 5471
BsrBI	CCGCTC	1 cut(s)	1553
BsrDI	GCAATG	8 cut(s)	626, 959, 3144, 3580, 4021, 4489, 6414, 6599
BsrFI	RCCGGY	2 cut(s)	2847, 6261
BsrGI	TGTACA	2 cut(s)	1510, 5086
BssAI	RCCGGY	2 cut(s)	2847, 6261
BssNAI	GTATAC	1 cut(s)	1194
BssNI	GRCGYC	1 cut(s)	4235
BssSI	CACGAG	2 cut(s)	396, 5805
BssT1I	CCWWGG	9 cut(s)	1923, 2596, 2725, 2790, 3022, 4923, 6266, 6635, 6709
Bst1107I	GTATAC	1 cut(s)	1194
Bst2BI	CACGAG	2 cut(s)	396, 5805
Bst6I	CTCTTC	9 cut(s)	136, 2674, 2771, 3074, 3123, 3511, 4996, 5568, 5586
BstACI	GRCGYC	1 cut(s)	4235
BstAFI	CTTAAG	2 cut(s)	4148, 5995
BstAPI	GCANNNNNTGC	5 cut(s)	1364, 2239, 2267, 4642, 6191
BstAUI	TGTACA	2 cut(s)	1510, 5086
BstBAI	YACGTR	1 cut(s)	4943
BstDSI	CCRYGG	6 cut(s)	1923, 3022, 4923, 5495, 6635, 6709
BstENI	CCTNNNNNAGG	4 cut(s)	1152, 2131, 4148, 5573
BstFNI	CGCG	3 cut(s)	4735, 5737, 6565
BstH2I	RGCGCY	5 cut(s)	130, 547, 2074, 2320, 4171
BstMCI	CGRYCG	2 cut(s)	2191, 5219
BstNSI	RCATGY	6 cut(s)	1451, 1713, 3621, 4291, 4912, 6521
BstPAI	GACNNNNGTC	2 cut(s)	2785, 3897
BstUI	CGCG	3 cut(s)	4735, 5737, 6565
BstV2I	GAAGAC	5 cut(s)	337, 1194, 3981, 6071, 6156
BstXI	CCANNNNNNTGG	1 cut(s)	2972
BstZ17I	GTATAC	1 cut(s)	1194
BsuI	GTATCC	4 cut(s)	257, 2155, 4133, 6407
BtgI	CCRYGG	6 cut(s)	1923, 3022, 4923, 5495, 6635, 6709
BtsI	GCAGTG	5 cut(s)	860, 4870, 5135, 6691, 6706
BveI	ACCTGC	6 cut(s)	774, 1156, 4678, 5119, 5368, 5789
CaiI	CAGNNNCTG	5 cut(s)	1411, 2435, 4349, 4577, 6473
Cfr10I	RCCGGY	2 cut(s)	2847, 6261
CseI	GACGC	3 cut(s)	4224, 5184, 6649
Csp6I	GTAC	8 cut(s)	427, 772, 1130, 1511, 5087, 5802, 6071, 6138
CspCI	CAANNNNNGTGG	2 cut(s)	6396, 6431
CviQI	GTAC	8 cut(s)	427, 772, 1130, 1511, 5087, 5802, 6071, 6138
DraI	TTTAAA	3 cut(s)	3057, 3768, 6595
DraIII	CACNNNGTG	2 cut(s)	1745, 5006
EaeI	YGGCCR	3 cut(s)	3157, 4703, 6263
Eam1104I	CTCTTC	9 cut(s)	136, 2674, 2771, 3074, 3123, 3511, 4996, 5568, 5586
EarI	CTCTTC	9 cut(s)	136, 2674, 2771, 3074, 3123, 3511, 4996, 5568, 5586
EciI	GGCGGA	1 cut(s)	3716
Ecl136II	GAGCTC	2 cut(s)	2715, 3198
Eco130I	CCWWGG	9 cut(s)	1923, 2596, 2725, 2790, 3022, 4923, 6266, 6635, 6709
Eco24I	GRGCYC	4 cut(s)	2000, 2717, 3200, 5689
Eco31I	GGTCTC	4 cut(s)	2773, 2842, 5026, 5471
Eco32I	GATATC	2 cut(s)	2017, 3906
Eco47I	GGWCC	4 cut(s)	2003, 5828, 5852, 6706
Eco47III	AGCGCT	1 cut(s)	545
Eco53kI	GAGCTC	2 cut(s)	2715, 3198
Eco57I	CTGAAG	6 cut(s)	331, 2292, 2405, 2730, 3477, 3800
Eco88I	CYCGRG	1 cut(s)	81
EcoICRI	GAGCTC	2 cut(s)	2715, 3198
EcoNI	CCTNNNNNAGG	4 cut(s)	1152, 2131, 4148, 5573
EcoO109I	RGGNCCY	4 cut(s)	1516, 3869, 5164, 5852
EcoRI	GAATTC	1 cut(s)	178
EcoRV	GATATC	2 cut(s)	2017, 3906
EcoT14I	CCWWGG	9 cut(s)	1923, 2596, 2725, 2790, 3022, 4923, 6266, 6635, 6709
EcoT22I	ATGCAT	2 cut(s)	2723, 4914
EcoT38I	GRGCYC	4 cut(s)	2000, 2717, 3200, 5689
ErhI	CCWWGG	9 cut(s)	1923, 2596, 2725, 2790, 3022, 4923, 6266, 6635, 6709
Esp3I	CGTCTC	2 cut(s)	4242, 6751
FalI	AAGNNNNNCTT	4 cut(s)	1017, 1049, 3213, 3245
FauI	CCCGC	1 cut(s)	737
FauNDI	CATATG	2 cut(s)	1102, 6014
FbaI	TGATCA	5 cut(s)	562, 1420, 1960, 2035, 5760
FblI	GTMKAC	3 cut(s)	1193, 2187, 6281
FriOI	GRGCYC	4 cut(s)	2000, 2717, 3200, 5689
FspI	TGCGCA	2 cut(s)	1109, 2232
GsaI	CCCAGC	4 cut(s)	858, 1897, 4500, 6498
GsuI	CTGGAG	2 cut(s)	1884, 5862
HaeII	RGCGCY	5 cut(s)	130, 547, 2074, 2320, 4171
HapII	CCGG	4 cut(s)	2101, 2848, 6040, 6262
HgaI	GACGC	3 cut(s)	4224, 5184, 6649
Hin1I	GRCGYC	1 cut(s)	4235
HincII	GTYRAC	5 cut(s)	40, 1013, 2188, 2625, 6291
HindII	GTYRAC	5 cut(s)	40, 1013, 2188, 2625, 6291
HindIII	AAGCTT	5 cut(s)	3051, 3212, 4206, 5069, 5081
HpaI	GTTAAC	1 cut(s)	40
HpaII	CCGG	4 cut(s)	2101, 2848, 6040, 6262
HpyCH4IV	ACGT	8 cut(s)	373, 1132, 1843, 4440, 4639, 4942, 5636, 6076
HpySE526I	ACGT	8 cut(s)	373, 1132, 1843, 4440, 4639, 4942, 5636, 6076
Hsp92I	GRCGYC	1 cut(s)	4235
Ksp22I	TGATCA	5 cut(s)	562, 1420, 1960, 2035, 5760
KspAI	GTTAAC	1 cut(s)	40
LguI	GCTCTTC	2 cut(s)	136, 2674
MaeII	ACGT	8 cut(s)	373, 1132, 1843, 4440, 4639, 4942, 5636, 6076
MbiI	CCGCTC	1 cut(s)	1553
MfeI	CAATTG	1 cut(s)	3042
MhlI	GDGCHC	6 cut(s)	403, 2000, 2717, 3200, 3862, 5689
MlsI	TGGCCA	1 cut(s)	3159
MluNI	TGGCCA	1 cut(s)	3159
Mox20I	TGGCCA	1 cut(s)	3159
Mph1103I	ATGCAT	2 cut(s)	2723, 4914
MroXI	GAANNNNTTC	5 cut(s)	802, 3520, 5267, 5700, 6384
MscI	TGGCCA	1 cut(s)	3159
MslI	CAYNNNNRTG	4 cut(s)	2127, 2970, 4286, 6105
Msp20I	TGGCCA	1 cut(s)	3159
MspA1I	CMGCKG	5 cut(s)	2894, 3533, 4346, 5930, 6801
MspCI	CTTAAG	2 cut(s)	4148, 5995
MspI	CCGG	4 cut(s)	2101, 2848, 6040, 6262
MssI	GTTTAAAC	1 cut(s)	3768
MunI	CAATTG	1 cut(s)	3042
Mva1269I	GAATGC	3 cut(s)	1373, 2678, 6372
MvnI	CGCG	3 cut(s)	4735, 5737, 6565
NciI	CCSGG	1 cut(s)	6040
NcoI	CCATGG	5 cut(s)	1923, 3022, 4923, 6635, 6709
NdeI	CATATG	2 cut(s)	1102, 6014
NmeAIII	GCCGAG	1 cut(s)	1777
NmuCI	GTSAC	8 cut(s)	858, 1912, 2702, 4408, 5352, 5560, 5659, 5674
NsbI	TGCGCA	2 cut(s)	1109, 2232
NsiI	ATGCAT	2 cut(s)	2723, 4914
NspI	RCATGY	6 cut(s)	1451, 1713, 3621, 4291, 4912, 6521
PaeI	GCATGC	1 cut(s)	6521
PaqCI	CACCTGC	1 cut(s)	4678
PasI	CCCWGGG	1 cut(s)	2660
PciI	ACATGT	3 cut(s)	1709, 3617, 4287
PciSI	GCTCTTC	2 cut(s)	136, 2674
PctI	GAATGC	3 cut(s)	1373, 2678, 6372
PdmI	GAANNNNTTC	5 cut(s)	802, 3520, 5267, 5700, 6384
PflMI	CCANNNNNTGG	3 cut(s)	329, 3207, 6473
PfoI	TCCNGGA	3 cut(s)	2407, 4225, 5899
PmeI	GTTTAAAC	1 cut(s)	3768
Ppu21I	YACGTR	1 cut(s)	4943
PpuMI	RGGWCCY	1 cut(s)	5852
PscI	ACATGT	3 cut(s)	1709, 3617, 4287
PshAI	GACNNNNGTC	2 cut(s)	2785, 3897
PshBI	ATTAAT	1 cut(s)	387
Psp124BI	GAGCTC	2 cut(s)	2717, 3200
Psp1406I	AACGTT	1 cut(s)	5636
Psp5II	RGGWCCY	1 cut(s)	5852
PspFI	CCCAGC	4 cut(s)	854, 1893, 4496, 6494
PspPPI	RGGWCCY	1 cut(s)	5852
PstI	CTGCAG	5 cut(s)	771, 4568, 4858, 5956, 6187
PstNI	CAGNNNCTG	5 cut(s)	1411, 2435, 4349, 4577, 6473
PvuII	CAGCTG	5 cut(s)	2894, 3533, 4346, 5930, 6801
RsaI	GTAC	8 cut(s)	428, 773, 1131, 1512, 5088, 5803, 6072, 6139
RsaNI	GTAC	8 cut(s)	427, 772, 1130, 1511, 5087, 5802, 6071, 6138
RseI	CAYNNNNRTG	4 cut(s)	2127, 2970, 4286, 6105
SacI	GAGCTC	2 cut(s)	2717, 3200
SalI	GTCGAC	1 cut(s)	2186
SapI	GCTCTTC	2 cut(s)	136, 2674
ScaI	AGTACT	2 cut(s)	6072, 6139
SduI	GDGCHC	6 cut(s)	403, 2000, 2717, 3200, 3862, 5689
SinI	GGWCC	4 cut(s)	2003, 5828, 5852, 6706
SmiMI	CAYNNNNRTG	4 cut(s)	2127, 2970, 4286, 6105
SpeI	ACTAGT	1 cut(s)	29
SphI	GCATGC	1 cut(s)	6521
SspI	AATATT	1 cut(s)	5449
SstI	GAGCTC	2 cut(s)	2717, 3200
StyI	CCWWGG	9 cut(s)	1923, 2596, 2725, 2790, 3022, 4923, 6266, 6635, 6709
TaiI	ACGT	8 cut(s)	376, 1135, 1846, 4443, 4642, 4945, 5639, 6079
TaqII	GACCGA	1 cut(s)	5205
TatI	WGTACW	4 cut(s)	1510, 5086, 6070, 6137
TauI	GCSGC	6 cut(s)	946, 1556, 2322, 3064, 4705, 5877
TseFI	GTSAC	8 cut(s)	858, 1912, 2702, 4408, 5352, 5560, 5659, 5674
Tsp45I	GTSAC	8 cut(s)	858, 1912, 2702, 4408, 5352, 5560, 5659, 5674
TspGWI	ACGGA	1 cut(s)	3909
Van91I	CCANNNNNTGG	3 cut(s)	329, 3207, 6473
Vha464I	CTTAAG	2 cut(s)	4148, 5995
VpaK11BI	GGWCC	4 cut(s)	2003, 5828, 5852, 6706
VspI	ATTAAT	1 cut(s)	387
XagI	CCTNNNNNAGG	4 cut(s)	1152, 2131, 4148, 5573
XbaI	TCTAGA	1 cut(s)	5095
XceI	RCATGY	6 cut(s)	1451, 1713, 3621, 4291, 4912, 6521
XcmI	CCANNNNNNNNNTGG	1 cut(s)	5635
XmiI	GTMKAC	3 cut(s)	1193, 2187, 6281
XmnI	GAANNNNTTC	5 cut(s)	802, 3520, 5267, 5700, 6384
ZrmI	AGTACT	2 cut(s)	6072, 6139
Zsp2I	ATGCAT	2 cut(s)	2723, 4914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.