Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0002990

Physical Location & Seq

Genomic Coordinates Reverse (-)

46920831 .. 46933935

Gene Structure 13105 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG255700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6765 bp

ATGCAAAGGTATCAGCCCACCGGCTTGACTGGTGCAGTTAGTAACAACACAATAGGTGGGGCATCAGGAAGGGATTCTGTCCGAGCTGAATCAGCTACGCTGCCAGCTCACTTACCTTTAAATTCTAGGCGCCCATCTCAAATAGCACCTTACAAATTGAAGTGTGATAAAGACCCTTTGAATGCTCGACTTGGGCCACCTGATTTCCATCCCCAAACCCCAAATTGCCCAGAGGAGACACTTACCAGGGAATATGTGCAATCTGGATACAGGGAGACTGTTGAAGGAATTGAGGAGGCTAAAGAGATATCACTTAGTCAGGCCCAATATTTCGGCAATCCTCTTGTCTTCAGGTGTAGAGAGGCAATCAAAAAACGTCTTAGAGCCATAAATGAATCTCGTGCTCAAAAGAGGAAGGCTGGTCAAGTGTATGGTGTTCCTCTTGCTGATTCACTCTTAACAAAGCCTGGTGTTTTTCCTGAACAAAGGCCCTGTGGTGACGACTTACGAAAAAAATGGATTGAGGGTTTATCACAACAACACAAACGCTTGCGTTCATTGGCTGATCATGTTCCTCATGGGTATAGGAAAAGATCTCTATTTGAAGTTCTTACAAGGAATAATGTTCCATTGCTGAGAGCAACTTGGTTTGTTAAAGTCACTTATCTTAATCAGATCCGGCCAGGTTCTTCTAGTATTTCTTCTGGGATACCTGATAAAACTCAGTTGTCTCGCACAGAGCACTGGACCAAAGATATCATTGAATACTTGCAGTACCTTCTCGATGAATTCTTTTCAAGAAACAGTTCACTTCCTGGTTCACACAACAGAGATCGATCACAACAAATGCTTTATGCTGGGTCAGTATCACAACGGAGTGATCCAGCATCATCAGTCCTTGATGGTGATGAGCCTTCCCTACATTTCAAGTGGTGGTATGTGGTGCGACTCCTGCAATGGCATCATGCTGAAGGGCTGCTTCTTCCTACACTTGTTATTGAATGGGTTCTACGTCAACTGCAGGAGAAGGAATTGCTCGAGATTGTGCAGTTGCTTTTGCCTATTATATATGGTGTTTTGGAGACTGTTGTCTTATCTCAAACTTATGTGCGCAATCTTGTCGGGACAGCTGTCCGTTTCATACGCGAACCTTCTCAAGGTGGATCCGATCTTGTGGATAACTCCAGAAGGGCGTATACAGTGTCTGCTCTGGTGGAGATGCTTCGTTATTTAATACTGTCTGTACCTGACTCATTTGTAGCTTTGGATTGCTTTCCTCTACCACCATGTGTAGTCTCTTATGCAGTAAACGATGGAAGTCTACAGAAGATGTCTGAGGATGTGAGAAAGATTAAAATTGGGTCAGCAGAAGTTGCTTCTGTATTCAGAAGCAAAGCTTTTGATGCTCAGTATCAATCCTTGGCCTTTGATCATGTTGTTTCATCCATTCAGAAGCGTGCTGACAATCTTGAAAAGGCTGCAAGCCCTAGCTATCCAGGCCATAGTATTGCTAAAGCTGTACAGGCCCTAGATAGGTCTCTTGCGCAGGGGGATGTTCTTGGGGTATATAGATTTCTGTTTGAAGATCCTTGCGAGGGAATTATAAATGAAAGTTGGGTTGCAGAAGTTAGTCCTTGCTTGAGAACATCATTGAAATGGATTGGTACCGTGAACTTGTCATTTGTTTGTTCGGTATTTTTCCTTTGTGAGTGGGCAACTTGTGATTTCAGGGATTTTCGAACTGCTCCTCCTTGTGAGCTAAAATTTACTGGCAGAAAAGATTTTTCTCAGGTACACATTGCAACTCGGCTTTTGATGCTAAAGATCCGAGACTTGCAAAGTTCACCTCAACATAAGAATGACAACCCTGCTAAAGGTTCCATTCAGCAGATTAATATTCCTGTCAGAAGTTCCATGGGAAGTTCTTATGAATCTAAAAATAAGTCAAGAAGTGTGCACCAGAGAAGTACAAAATCATCAAATATATTTGAGAGCCCGGGCCCTTTACATGATGTCATAGTTTGTTGGATTGATCAACATGATGCAGGCAAAGGAGAAGGTTTGAAGCGCCTTCAATTTCTTGTTATTGAACTCATACGTTCTGGTATTTTTTACCCCCATGTCTATGTTAGACAGCTGATAGTTAGTGGCATTATGGATATAAATGGACCAGTGACTGAATCAGACAGAAGGAAGAGGCATTATCAGATCTTGAAGTTGTTGCCTGGGGTCTTTGTGCATGATGCTCTGGAAGAAGCAGTGGTTGCTGAAGGCCCAAAACTTTTGGAGGCTATTAGTTCTTACTCAAATGAACGGCGCCTCATACTTAATGGGTTCCTTGGTGATCAAAATAAAAATTTGAGTATGAAGTCAGCTCTGAAGCAAAAGAACAATGCCATCCCTGGAAAGGATGGTGGTTTGCCAGTTTCTGCTGATCAGTGGAAGACTGTTGAGTTGTCATCCAGCATATTGTCTGGCAAAAGTGGCAAGAGGGGCAAAATTGATGCTGATGTTGAAGAACTAAAGGAAGCAATCTCACTATTGTTACAACTGCCATATAGTTCCACCCCACCAACAGATACAGGACTTGAAGAGTCCCAAGGGAGTGTGAAGAGGCCTTTTGGGTCGATCTCTAACAAGATGGATCTTGGAGAAGGCACCCCTGGGTGTGAAGAATGCAGAAGAGCAAAGAGGCAAAAAGTGAGTGACGAAAGGAGCTCATATATCCAAGGAAATTCTCCAATTCCTTCAGATGATGAAGATACTTGGTGGATGAGGAAGAGACCTAAATCCTCAGAGCCGTTGAAGGTTGATCCACCAGTCAAGCTAACTAAACAGGTATCTAGGAATCGGCAGAAAATGGTTCGCAAAACTCAAAGTCTTGCTCAACTAGCAGCTGCTAGGATTGAAGGGAGCCAGGGAGCTTCTACAAGTCATGTCTGCAATAATAAGATAAGTTGTCCTCATCATAGATCTGGACTAGAGGGAGAACCTCCGAAGCCTACAGATACGACCAAAACGAACCATGCTGGAGATATTGTTTCTATTGGAAAAACTTTAAAGCGGCTGCGATTTGCAGAGAAGAGGACTATTACTGTTTGGTTAATGACTAATATCAGGCAGCTTGTTGAAGAGACTGAAAAGACAATTGCTAAGGTTGGCCAGTTTGGTAGGAATTTCACACCAGTTGATGATAGGAGCTCCATACGGTGGAAGCTCGGTGAGGATGAACTTTCTGCTGCACTGTATTTAATGGATGTTTCAGATGATTTAGTTTCAGCAGTAAAGTTTCTTCTTTGGCTGCTGCCAAAGGTTATTACTAGCCCCAGTTCTACTATCCATAGTGGGAGAAACATTCTACTGCTGCCAAGAAATGTTGAAGGCCAAGTATGTGAAGTTGGGGAGGCATTTCTAATATCGTCCCTCCGAAGGTATGAGAACATACTTCTTGCAACAGATCTCATTCCAGAAGTCTTATCAGCGACAATGCTCCGTGCTTCAGCAGTGGTGGCATCTAACGGAAGAGTTTCAGGTTCAGCTGCCTTAGTTTACAGTCGGTATCTGTTGAAGCGGTATGGCAATGTGGCTAGTGTGATTGAATGGGAGAAGAGTTTCAAGGTAACATGTGATAAGAGGCTTTATTCTGAACTTGAATCTGGGCAAACAGTGGATGGAGAGTTAGGGTTCGCGCTTGGTGTTCCATCAGGAGTTGAGGATCTTGATGATTATTTTCGCCAAAAGATTAGTGGTGTCCGGTTATCTCGGGTGGGTTTGAACATGAGAGAGATTGTACAAAAAAATGTGAATGTTGACGATGCCTTTCACTATTTTTCGGGCAAGGAGAGAAAACTCTTTGCTGGCAGCACTCCTAAAGCCCCTGCAATTGAAAAATGGGATGACGGGTATCAGATTGCTCATAAAATAATAACTGAACTGATGGACTGCATTCGGCAAACTGGTGGTGCTGCTCAAGAAGGCGACCCAACTTTGGTGTCATCCGCTGTTTCTGCTATTGTTGGTAATGTAGGGTCAATTCTAGCAAAAGTTTCTGATTTCAGACCAGGGGGTTATCCATCTGCCACTGATTCCCTAAACTTTGCTAGACGTATTCTACGCATCCATATAAGTTGCCTATGCCTTCTCAAGGAAGCTCTTGGAGAGCGTCAAACTCGTGTATTTGAGGTAGCTCTTGCCACAGAAGCTTGTTCTGCTCTTGCTGGGGTTTTTTCTCCTGGAAAGGGTTCTCGGAATCAATATCAGTTATCTCCTGAATCCCATGATTCCAATACAAAAGTTGCAGCTGCTGTTTCTGCTCTAATCATCGGGGCAGTTATTAATGGGATTACTAGCTTGGAGAGGTTGGTGACTGTGTTCAGGTTTAAGGAAAAGTTGGATATAATTCAATTTGTGAGGAATAGTAGATCCAACTCAAATGGTAATGCTCGTACTGCTGGGGCACTTAAGGGTGATAACTCACTTGAAGTTTATGTTCATTGGTTTAGATTGCTTGTTGGAAACTGCAGAACTGTTTCGGATGGAATGGTTGTGGAACTCTTGGGTGAACCATCCATAATAGCTCTTTCCAGGATGCAGCGACTGCTTCCTCTTCGTTTGGTTTTTCCACCTGCTTATTCAATATTTGCTTTTGTTATATGGCGGCCATTCCTTCTGAACACTTGCCTTGCAGTTCGTGAGGATTTTAATCAGTCATATCAATCTTTAGCAACAGCTATAGGTGATGTTATTAAACATTTGCCTTTTCGTGATGTTTGTTTGAGAGATGGTCAGGGTTTCTATGACATTGTAGCAGCAGATAGCAGTGATGCTGAATTTGCAGCCATGCTGGAGTTGAACGGTTCAGACATTCATTTTAAATCCATGGCTTTTGTTCCATTACGTGCAAGGCTTTTTCTGAATGCCATTCTTGACTGTAAGTTGCCAAACTCTTTATTTCCACAGGCGGAAGGAAATCAGGTATCTGGACAGGGCGAAACTAAAGTTCATTATTCAGAGCGTGAAACAAAGCTTGTAGATAAGCTTGTCCATATTTTAGATACCCTTCAACCTGCAAAATTTCATTGGCAATGGGTAGAGCTCAGGCTCCTTTTGAATGAACAAGCCCTCATTGAAAAACTAGAGACACAGGACATGTCTTTAGTGGATGCCATACGTTCATCCTCACCTAGTCCAGAAAAGGCTGCTGCCTCTGAAAATGAGAAATATTTCATTGAAATCATCCTTACAAGATTATTGGTTAGACCTGATGCTGCACCCCTTTTCTCAGATGTGGTTCATCTCTTTGGGAGGTCTCTTGCAGATTCTATGTTGTTGCAGGTTAAATGGTTCCTGGGTGGTCCAGATGTTCTTTTTGGACGAAAAACCATTAGACAACGGCTTATGAATATTGGCGAGAGTAAAGGTCTCTCGACTAAGACCCACTTTTGGAAACCTTGGGGCTGGTTTACTTCTGAATCTGATCTTCTGACAAACAGGGGAGATAAGAAGAAGTTTGAAGTCACCTCCCTTGAAGAGGGTGAGATGGTTGAAGAGGGGACAGAGTCAAAAAGGCATGGGAAAGGGTCCAGTCAATTATTTGACAATGAAGGCCAGAGTGTCTCCCAGCAGCATGTGACAGAGAGGGCTCTCATTGAATTACTTCTTCCTTGCATAGATCAAAGTTCAGATGACTCACGAAATACATTTGCAAATGATTTGATCAAGCAGTTGAGTAATATCGAGCAACAAATTAGTGCTGTGACTCGCGGGACAAGCAAGCAAGCAGGACAAACTCCTTCTGGAATTGAAGGTCCCACAAGCAAAGGAAATAATCGCAAAGGTATAAGGGGTGGCAGTCCTGGATTAGCTAGACGTGCAGCAGCAGCAGCAGCAGCAGATTCTGCTCCACCGTCCCCTGCAGCTTTGCGTGCTTCTATGTCATTGAGATTGCAGTTACTCTTGAGATTGCTTCCAATCATCTATGCAGATAGGGAGCCATCAGCGCGGAACATGAGGCATGGGCTTGCCACCGTTATATTACGCCTTCTAGGAAACAGAGTTGTGCATGAGGATGCAGACCCATCCTTCACTCCCTCACAGAGTTCTTTATCCAAGAGGGAGTCAGATTCTTCAATGGATGCTGCCTCTGCTGCTTTCGCAGACTTGTCAAGCGAAAGTCTCTTTGATGAGTTATTGCTGGTTCTGCACGGGCTGTTAAGCAGTTGCCAGCCAAGTTGGTTGAGGTCTACTAAGCCAACAAAAGAAAGTGGGAAAGGTTTTGTTGCATTTGATCGTGAACTGGCAGAGAATTTGCAGAATGACTTGGATCGCATGCAGCTGCCTGACACTGTTAGGTGGCGTATTCAAACTGCAATGCCGGTAATAATTCCTTCAATTCGGTGCGTTGTGTCGTGCCAGCCACCACCAGTTCCAAACATGGCTTTGGCTGTTCTTCAGTCCAGCACATCAAATTCCGTGATTTACTCGGGAAACTCGAACCCACTGCAGAAAAATCAGGTTCCTCTGGCACGAACAGTAACTAGTGTAACGGGGAAGTCGAAGCCATTGCCATCCCAGGATTATGATATGGAGATTGACCCATGGACACTCCTGGAAGATGGTGCAGGATCAGGCCCGAGCTCAGGCAACAGTGCTTTAATAGGCAGTGCAGATCATGGAAATCTTCGAGCATCTAGCTGGCTTAAGGGGGCTGTAAGAGTAAGACGGAAGGATCTCACATACATTGGTGCTGTGGACGATGACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006352 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016592 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:0070013 GO:0071704 GO:0090213 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med12

Protein Analysis

2254

Amino Acids

249.57

Weight (kDa)

8.82

Isoelectric Point (pI)

49.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med12	PF09497	159 - 219	4.9e-20	Transcription mediator complex subunit Med12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00450 AT4G00450 AT4G00450
fragaria_vesca	FvH4_7g14270 FvH4_7g14270 FvH4_7g14270
malus_domestica	MD01G1060600.v1.1 MD07G1137100.v1.1
prunus_persica	Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1
pyrus_communis	pycom01g09080 pycom07g13490
rosa_chinensis	RchiOBHm_Chr1g0355261
rosa_laevigata	RLG00000028166
rosa_multiflora	Rmu_sc0003115.1_g000044
rosa_roxburghii	Rroxscaffold_4G00300550
rosa_rugosa	Rorug01G0245800 Rorug01G0245900 Rorug01G0246000 Rorug01G0246100 Rorug01G0246200 Rorug01G0246200 Rorug01G0246200
rosa_samantha	Rh1AG258300 Rh1BG227900 Rh1CG242000 Rh1DG255700
rosa_wichuraiana	Rw1G022800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1604
AarI	CACCTGC	1 cut(s)	4633
Acc16I	TGCGCA	2 cut(s)	1112, 1545
Acc36I	ACCTGC	3 cut(s)	4633, 5074, 5323
Acc65I	GGTACC	1 cut(s)	1664
AccB1I	GGYRCC	4 cut(s)	129, 1664, 2316, 2657
AccB7I	CCANNNNNTGG	1 cut(s)	3210
AccI	GTMKAC	3 cut(s)	1196, 1321, 6242
AccII	CGCG	4 cut(s)	1146, 3689, 5763, 6001
AciI	CCGC	7 cut(s)	3064, 3571, 3999, 4657, 4961, 5763, 6001
AcoI	YGGCCR	3 cut(s)	680, 3160, 4658
AcuI	CTGAAG	7 cut(s)	334, 990, 2289, 2399, 2733, 3483, 6434
AcyI	GRCGYC	2 cut(s)	130, 2317
AdeI	CACNNNGTG	1 cut(s)	1289
AfaI	GTAC	8 cut(s)	776, 1245, 1521, 1666, 1794, 1969, 3792, 4447
AflII	CTTAAG	2 cut(s)	4460, 6698
AflIII	ACRYGT	2 cut(s)	3623, 5148
AhlI	ACTAGT	1 cut(s)	6536
AjiI	CACGTC	1 cut(s)	5870
AloI	GAACNNNNNNTCC	2 cut(s)	1732, 1764
Alw21I	GWGCWC	7 cut(s)	406, 744, 1959, 2720, 3203, 5097, 6638
Alw44I	GTGCAC	1 cut(s)	1955
AlwNI	CAGNNNCTG	6 cut(s)	1205, 2177, 2429, 4304, 4597, 5897
Ama87I	CYCGRG	5 cut(s)	1037, 1996, 3762, 6481, 6631
ApaI	GGGCCC	1 cut(s)	2003
ApaLI	GTGCAC	1 cut(s)	1955
AseI	ATTAAT	2 cut(s)	1893, 4335
Asp700I	GAANNNNTTC	9 cut(s)	73, 805, 1005, 3526, 3611, 4240, 4528, 5222, 5655
Asp718I	GGTACC	1 cut(s)	1664
AspLEI	GCGC	7 cut(s)	132, 1113, 1546, 2070, 2319, 3691, 6001
AsuC2I	CCSGG	2 cut(s)	1997, 1998
AsuII	TTCGAA	1 cut(s)	1738
AvaI	CYCGRG	5 cut(s)	1037, 1996, 3762, 6481, 6631
AvaII	GGWCC	5 cut(s)	747, 2168, 5354, 5580, 5807
BaeGI	GKGCMC	3 cut(s)	1959, 2003, 4459
BalI	TGGCCA	1 cut(s)	3162
BamHI	GGATCC	1 cut(s)	1163
BanI	GGYRCC	4 cut(s)	129, 1664, 2316, 2657
BanII	GRGCYC	7 cut(s)	1997, 2003, 2720, 3203, 5097, 5644, 6638
BarI	GAAGNNNNNNTAC	4 cut(s)	1180, 1212, 3276, 3308
BauI	CACGAG	2 cut(s)	399, 4170
BbsI	GAAGAC	2 cut(s)	340, 2450
Bbv12I	GWGCWC	7 cut(s)	406, 744, 1959, 2720, 3203, 5097, 6638
BceAI	ACGGC	3 cut(s)	2330, 2785, 5408
BcgI	CGANNNNNNTGC	6 cut(s)	1102, 1136, 6481, 6515, 6544, 6578
BciVI	GTATCC	2 cut(s)	260, 702
BclI	TGATCA	6 cut(s)	565, 1429, 2032, 2344, 2434, 5715
BcnI	CCSGG	2 cut(s)	1997, 1998
BcuI	ACTAGT	1 cut(s)	6536
BfmI	CTRYAG	7 cut(s)	1019, 1322, 3003, 4519, 4731, 5913, 6500
BfoI	RGCGCY	3 cut(s)	133, 2071, 2320
BfrI	CTTAAG	2 cut(s)	4460, 6698
BfuAI	ACCTGC	3 cut(s)	4633, 5074, 5323
BfuI	GTATCC	2 cut(s)	260, 702
BglII	AGATCT	4 cut(s)	593, 2208, 2972, 3457
Bme18I	GGWCC	5 cut(s)	747, 2168, 5354, 5580, 5807
BmeT110I	CYCGRG	5 cut(s)	1037, 1996, 3762, 6481, 6631
BmgBI	CACGTC	1 cut(s)	5870
BmrI	ACTGGG	1 cut(s)	3321
BmuI	ACTGGG	1 cut(s)	3321
BoxI	GACNNNNGTC	2 cut(s)	1088, 1130
BpiI	GAAGAC	2 cut(s)	340, 2450
BpmI	CTGGAG	3 cut(s)	1168, 3051, 4865
Bpu10I	CCTNAGC	3 cut(s)	3153, 5096, 6637
Bpu14I	TTCGAA	1 cut(s)	1738
BpuEI	CTTGAG	5 cut(s)	1140, 1660, 3954, 4127, 5977
BpuMI	CCSGG	2 cut(s)	1997, 1998
Bsa29I	ATCGAT	1 cut(s)	835
BsaAI	YACGTR	1 cut(s)	4898
BsaBI	GATNNNNATC	2 cut(s)	207, 4701
BsaHI	GRCGYC	2 cut(s)	130, 2317
BsaI	GGTCTC	4 cut(s)	1542, 2776, 5313, 5426
BsaWI	WCCGGW	1 cut(s)	3753
BsaXI	ACNNNNNCTCC	4 cut(s)	1732, 1762, 3024, 3054
Bse118I	RCCGGY	2 cut(s)	20, 6373
Bse3DI	GCAATG	7 cut(s)	629, 962, 1797, 3586, 5090, 6375, 6560
Bse8I	GATNNNNATC	2 cut(s)	207, 4701
BseCI	ATCGAT	1 cut(s)	835
BseJI	GATNNNNATC	2 cut(s)	207, 4701
BseMI	GCAATG	7 cut(s)	629, 962, 1797, 3586, 5090, 6375, 6560
BseMII	CTCAG	9 cut(s)	626, 737, 1326, 1421, 1802, 2808, 5110, 5295, 6651
BseRI	GAGGAG	3 cut(s)	248, 308, 1737
BseSI	GKGCMC	3 cut(s)	1959, 2003, 4459
BseYI	CCCAGC	4 cut(s)	857, 4217, 4451, 5619
BsgI	GTGCAG	8 cut(s)	54, 1067, 3225, 5253, 5892, 6185, 6639, 6684
Bsh1236I	CGCG	4 cut(s)	1146, 3689, 5763, 6001
BshNI	GGYRCC	4 cut(s)	129, 1664, 2316, 2657
BshVI	ATCGAT	1 cut(s)	835
BsiHKAI	GWGCWC	7 cut(s)	406, 744, 1959, 2720, 3203, 5097, 6638
BsiHKCI	CYCGRG	5 cut(s)	1037, 1996, 3762, 6481, 6631
BsiSI	CCGG	5 cut(s)	21, 679, 1997, 3754, 6374
BslFI	GGGAC	7 cut(s)	1138, 2581, 3406, 5566, 5779, 5793, 5893
BsmFI	GGGAC	7 cut(s)	1138, 2581, 3406, 5566, 5779, 5793, 5893
BsmI	GAATGC	4 cut(s)	187, 2681, 3945, 4921
Bso31I	GGTCTC	4 cut(s)	1542, 2776, 5313, 5426
BsoBI	CYCGRG	5 cut(s)	1037, 1996, 3762, 6481, 6631
Bsp119I	TTCGAA	1 cut(s)	1738
Bsp120I	GGGCCC	1 cut(s)	1999
Bsp1407I	TGTACA	2 cut(s)	1519, 3790
Bsp19I	CCATGG	3 cut(s)	1914, 4878, 6596
BspACI	CCGC	7 cut(s)	3064, 3571, 3999, 4657, 4961, 5763, 6001
BspCNI	CTCAG	9 cut(s)	627, 736, 1327, 1420, 1801, 2807, 5109, 5294, 6650
BspDI	ATCGAT	1 cut(s)	835
BspFNI	CGCG	4 cut(s)	1146, 3689, 5763, 6001
BspMAI	CTGCAG	4 cut(s)	1023, 4523, 5917, 6504
BspMI	ACCTGC	3 cut(s)	4633, 5074, 5323
BspQI	GCTCTTC	1 cut(s)	2677
BspT104I	TTCGAA	1 cut(s)	1738
BspT107I	GGYRCC	4 cut(s)	129, 1664, 2316, 2657
BspTI	CTTAAG	2 cut(s)	4460, 6698
BspTNI	GGTCTC	4 cut(s)	1542, 2776, 5313, 5426
BsrDI	GCAATG	7 cut(s)	629, 962, 1797, 3586, 5090, 6375, 6560
BsrFI	RCCGGY	2 cut(s)	20, 6373
BsrGI	TGTACA	2 cut(s)	1519, 3790
BssAI	RCCGGY	2 cut(s)	20, 6373
BssNAI	GTATAC	1 cut(s)	1197
BssNI	GRCGYC	2 cut(s)	130, 2317
BssSI	CACGAG	2 cut(s)	399, 4170
BssT1I	CCWWGG	8 cut(s)	1419, 1914, 2338, 2599, 2728, 4878, 5450, 6596
Bst1107I	GTATAC	1 cut(s)	1197
Bst2BI	CACGAG	2 cut(s)	399, 4170
BstACI	GRCGYC	2 cut(s)	130, 2317
BstAFI	CTTAAG	2 cut(s)	4460, 6698
BstAPI	GCANNNNNTGC	4 cut(s)	1373, 2264, 4597, 5897
BstAUI	TGTACA	2 cut(s)	1519, 3790
BstBAI	YACGTR	1 cut(s)	4898
BstBI	TTCGAA	1 cut(s)	1738
BstDSI	CCRYGG	3 cut(s)	1914, 4878, 6596
BstENI	CCTNNNNNAGG	4 cut(s)	1155, 1872, 4142, 5528
BstFNI	CGCG	4 cut(s)	1146, 3689, 5763, 6001
BstH2I	RGCGCY	3 cut(s)	133, 2071, 2320
BstHHI	GCGC	7 cut(s)	132, 1113, 1546, 2070, 2319, 3691, 6001
BstNSI	RCATGY	4 cut(s)	3627, 5152, 5630, 6331
BstPAI	GACNNNNGTC	2 cut(s)	1088, 1130
BstSFI	CTRYAG	7 cut(s)	1019, 1322, 3003, 4519, 4731, 5913, 6500
BstSLI	GKGCMC	3 cut(s)	1959, 2003, 4459
BstUI	CGCG	4 cut(s)	1146, 3689, 5763, 6001
BstV2I	GAAGAC	2 cut(s)	340, 2450
BstZ17I	GTATAC	1 cut(s)	1197
Bsu15I	ATCGAT	1 cut(s)	835
BsuI	GTATCC	2 cut(s)	260, 702
BsuTUI	ATCGAT	1 cut(s)	835
BtgI	CCRYGG	3 cut(s)	1914, 4878, 6596
BtrI	CACGTC	1 cut(s)	5870
BtsI	GCAGTG	5 cut(s)	2265, 3510, 4825, 6497, 6667
BveI	ACCTGC	3 cut(s)	4633, 5074, 5323
CaiI	CAGNNNCTG	6 cut(s)	1205, 2177, 2429, 4304, 4597, 5897
CfoI	GCGC	7 cut(s)	132, 1113, 1546, 2070, 2319, 3691, 6001
Cfr10I	RCCGGY	2 cut(s)	20, 6373
Cfr9I	CCCGGG	1 cut(s)	1996
ClaI	ATCGAT	1 cut(s)	835
CseI	GACGC	1 cut(s)	4151
Csp6I	GTAC	8 cut(s)	775, 1244, 1520, 1665, 1793, 1968, 3791, 4446
CviQI	GTAC	8 cut(s)	775, 1244, 1520, 1665, 1793, 1968, 3791, 4446
DinI	GGCGCC	2 cut(s)	131, 2318
DraI	TTTAAA	3 cut(s)	120, 3060, 4873
DraIII	CACNNNGTG	1 cut(s)	1289
EaeI	YGGCCR	3 cut(s)	680, 3160, 4658
EciI	GGCGGA	1 cut(s)	4976
Ecl136II	GAGCTC	4 cut(s)	2718, 3201, 5095, 6636
Eco130I	CCWWGG	8 cut(s)	1419, 1914, 2338, 2599, 2728, 4878, 5450, 6596
Eco147I	AGGCCT	1 cut(s)	2617
Eco24I	GRGCYC	7 cut(s)	1997, 2003, 2720, 3203, 5097, 5644, 6638
Eco31I	GGTCTC	4 cut(s)	1542, 2776, 5313, 5426
Eco32I	GATATC	2 cut(s)	309, 757
Eco47I	GGWCC	5 cut(s)	747, 2168, 5354, 5580, 5807
Eco53kI	GAGCTC	4 cut(s)	2718, 3201, 5095, 6636
Eco57I	CTGAAG	7 cut(s)	334, 990, 2289, 2399, 2733, 3483, 6434
Eco88I	CYCGRG	5 cut(s)	1037, 1996, 3762, 6481, 6631
EcoICRI	GAGCTC	4 cut(s)	2718, 3201, 5095, 6636
EcoNI	CCTNNNNNAGG	4 cut(s)	1155, 1872, 4142, 5528
EcoO109I	RGGNCCY	4 cut(s)	489, 1525, 2000, 5807
EcoRI	GAATTC	1 cut(s)	788
EcoRV	GATATC	2 cut(s)	309, 757
EcoT14I	CCWWGG	8 cut(s)	1419, 1914, 2338, 2599, 2728, 4878, 5450, 6596
EcoT38I	GRGCYC	7 cut(s)	1997, 2003, 2720, 3203, 5097, 5644, 6638
EgeI	GGCGCC	2 cut(s)	131, 2318
EheI	GGCGCC	2 cut(s)	131, 2318
ErhI	CCWWGG	8 cut(s)	1419, 1914, 2338, 2599, 2728, 4878, 5450, 6596
FalI	AAGNNNNNCTT	6 cut(s)	963, 995, 1381, 1413, 5423, 5455
FaqI	GGGAC	7 cut(s)	1138, 2581, 3406, 5566, 5779, 5793, 5893
FauI	CCCGC	1 cut(s)	5756
FbaI	TGATCA	6 cut(s)	565, 1429, 2032, 2344, 2434, 5715
FblI	GTMKAC	3 cut(s)	1196, 1321, 6242
FriOI	GRGCYC	7 cut(s)	1997, 2003, 2720, 3203, 5097, 5644, 6638
FspI	TGCGCA	2 cut(s)	1112, 1545
GlaI	GCGC	7 cut(s)	131, 1112, 1545, 2069, 2318, 3690, 6000
GsaI	CCCAGC	4 cut(s)	861, 4221, 4455, 5623
GsuI	CTGGAG	3 cut(s)	1168, 3051, 4865
HaeII	RGCGCY	3 cut(s)	133, 2071, 2320
HapII	CCGG	5 cut(s)	21, 679, 1997, 3754, 6374
HgaI	GACGC	1 cut(s)	4151
HhaI	GCGC	7 cut(s)	132, 1113, 1546, 2070, 2319, 3691, 6001
Hin1I	GRCGYC	2 cut(s)	130, 2317
Hin6I	GCGC	7 cut(s)	130, 1111, 1544, 2068, 2317, 3689, 5999
HinP1I	GCGC	7 cut(s)	130, 1111, 1544, 2068, 2317, 3689, 5999
HincII	GTYRAC	2 cut(s)	1016, 3811
HindII	GTYRAC	2 cut(s)	1016, 3811
HindIII	AAGCTT	4 cut(s)	1395, 4200, 5024, 5036
HpaII	CCGG	5 cut(s)	21, 679, 1997, 3754, 6374
HpyCH4IV	ACGT	7 cut(s)	376, 1012, 2098, 4105, 4897, 5170, 5869
HpySE526I	ACGT	7 cut(s)	376, 1012, 2098, 4105, 4897, 5170, 5869
Hsp92I	GRCGYC	2 cut(s)	130, 2317
HspAI	GCGC	7 cut(s)	130, 1111, 1544, 2068, 2317, 3689, 5999
KasI	GGCGCC	2 cut(s)	129, 2316
KpnI	GGTACC	1 cut(s)	1668
Ksp22I	TGATCA	6 cut(s)	565, 1429, 2032, 2344, 2434, 5715
LguI	GCTCTTC	1 cut(s)	2677
MaeII	ACGT	7 cut(s)	376, 1012, 2098, 4105, 4897, 5170, 5869
MfeI	CAATTG	2 cut(s)	3147, 3882
MlsI	TGGCCA	1 cut(s)	3162
MluNI	TGGCCA	1 cut(s)	3162
Mly113I	GGCGCC	2 cut(s)	130, 2317
MlyI	GAGTC	7 cut(s)	942, 1244, 2603, 5567, 5681, 5752, 6125
MmeI	TCCRAC	4 cut(s)	2006, 4371, 4449, 4492
Mox20I	TGGCCA	1 cut(s)	3162
MroXI	GAANNNNTTC	9 cut(s)	73, 805, 1005, 3526, 3611, 4240, 4528, 5222, 5655
MscI	TGGCCA	1 cut(s)	3162
MslI	CAYNNNNRTG	4 cut(s)	1654, 1857, 2124, 6066
Msp20I	TGGCCA	1 cut(s)	3162
MspA1I	CMGCKG	8 cut(s)	1130, 2137, 2897, 3539, 4001, 4301, 6334, 6762
MspCI	CTTAAG	2 cut(s)	4460, 6698
MspI	CCGG	5 cut(s)	21, 679, 1997, 3754, 6374
MunI	CAATTG	2 cut(s)	3147, 3882
Mva1269I	GAATGC	4 cut(s)	187, 2681, 3945, 4921
MvnI	CGCG	4 cut(s)	1146, 3689, 5763, 6001
NarI	GGCGCC	2 cut(s)	130, 2317
NciI	CCSGG	2 cut(s)	1997, 1998
NcoI	CCATGG	3 cut(s)	1914, 4878, 6596
NmeAIII	GCCGAG	1 cut(s)	1786
NmuCI	GTSAC	8 cut(s)	497, 658, 2173, 2705, 4363, 5515, 5629, 5755
NsbI	TGCGCA	2 cut(s)	1112, 1545
NspI	RCATGY	4 cut(s)	3627, 5152, 5630, 6331
NspV	TTCGAA	1 cut(s)	1738
PaeI	GCATGC	1 cut(s)	6331
PaeR7I	CTCGAG	1 cut(s)	1037
PaqCI	CACCTGC	1 cut(s)	4633
PasI	CCCWGGG	1 cut(s)	2663
PceI	AGGCCT	1 cut(s)	2617
PciI	ACATGT	2 cut(s)	3623, 5148
PciSI	GCTCTTC	1 cut(s)	2677
PcsI	WCGNNNNNNNCGW	3 cut(s)	3017, 6488, 6551
PctI	GAATGC	4 cut(s)	187, 2681, 3945, 4921
PdmI	GAANNNNTTC	9 cut(s)	73, 805, 1005, 3526, 3611, 4240, 4528, 5222, 5655
PflFI	GACNNNGTC	1 cut(s)	5557
PflMI	CCANNNNNTGG	1 cut(s)	3210
PfoI	TCCNGGA	4 cut(s)	4231, 4583, 5854, 6606
PleI	GAGTC	7 cut(s)	942, 1244, 2602, 5566, 5681, 5752, 6124
PluTI	GGCGCC	2 cut(s)	133, 2320
PpsI	GAGTC	7 cut(s)	942, 1244, 2602, 5566, 5681, 5752, 6124
Ppu21I	YACGTR	1 cut(s)	4898
PpuMI	RGGWCCY	1 cut(s)	5807
PscI	ACATGT	2 cut(s)	3623, 5148
PshAI	GACNNNNGTC	2 cut(s)	1088, 1130
PshBI	ATTAAT	2 cut(s)	1893, 4335
PsiI	TTATAA	1 cut(s)	1604
Psp124BI	GAGCTC	4 cut(s)	2720, 3203, 5097, 6638
Psp5II	RGGWCCY	1 cut(s)	5807
PspFI	CCCAGC	4 cut(s)	857, 4217, 4451, 5619
PspOMI	GGGCCC	1 cut(s)	1999
PspPPI	RGGWCCY	1 cut(s)	5807
PstI	CTGCAG	4 cut(s)	1023, 4523, 5917, 6504
PstNI	CAGNNNCTG	6 cut(s)	1205, 2177, 2429, 4304, 4597, 5897
PsyI	GACNNNGTC	1 cut(s)	5557
PvuII	CAGCTG	7 cut(s)	1130, 2137, 2897, 3539, 4301, 6334, 6762
RsaI	GTAC	8 cut(s)	776, 1245, 1521, 1666, 1794, 1969, 3792, 4447
RsaNI	GTAC	8 cut(s)	775, 1244, 1520, 1665, 1793, 1968, 3791, 4446
RseI	CAYNNNNRTG	4 cut(s)	1654, 1857, 2124, 6066
SacI	GAGCTC	4 cut(s)	2720, 3203, 5097, 6638
SapI	GCTCTTC	1 cut(s)	2677
SchI	GAGTC	7 cut(s)	942, 1244, 2603, 5567, 5681, 5752, 6125
SfcI	CTRYAG	7 cut(s)	1019, 1322, 3003, 4519, 4731, 5913, 6500
SfoI	GGCGCC	2 cut(s)	131, 2318
Sfr274I	CTCGAG	1 cut(s)	1037
SfuI	TTCGAA	1 cut(s)	1738
SinI	GGWCC	5 cut(s)	747, 2168, 5354, 5580, 5807
SlaI	CTCGAG	1 cut(s)	1037
SmaI	CCCGGG	1 cut(s)	1998
SmiMI	CAYNNNNRTG	4 cut(s)	1654, 1857, 2124, 6066
SmlI	CTYRAG	8 cut(s)	1037, 1155, 1639, 3969, 4142, 4460, 5956, 6698
SmoI	CTYRAG	8 cut(s)	1037, 1155, 1639, 3969, 4142, 4460, 5956, 6698
SpeI	ACTAGT	1 cut(s)	6536
SphI	GCATGC	1 cut(s)	6331
SrfI	GCCCGGGC	1 cut(s)	1998
SseBI	AGGCCT	1 cut(s)	2617
SsiI	CCGC	7 cut(s)	3064, 3571, 3999, 4657, 4961, 5763, 6001
SspDI	GGCGCC	2 cut(s)	129, 2316
SspI	AATATT	5 cut(s)	329, 1897, 4638, 5222, 5404
SstI	GAGCTC	4 cut(s)	2720, 3203, 5097, 6638
StuI	AGGCCT	1 cut(s)	2617
StyI	CCWWGG	8 cut(s)	1419, 1914, 2338, 2599, 2728, 4878, 5450, 6596
TaiI	ACGT	7 cut(s)	379, 1015, 2101, 4108, 4900, 5173, 5872
TatI	WGTACW	3 cut(s)	1519, 1967, 3790
TauI	GCSGC	2 cut(s)	3067, 4660
TseFI	GTSAC	8 cut(s)	497, 658, 2173, 2705, 4363, 5515, 5629, 5755
Tsp45I	GTSAC	8 cut(s)	497, 658, 2173, 2705, 4363, 5515, 5629, 5755
TspGWI	ACGGA	6 cut(s)	889, 1124, 3482, 3534, 6460, 6736
TspMI	CCCGGG	1 cut(s)	1996
Tth111I	GACNNNGTC	1 cut(s)	5557
Van91I	CCANNNNNTGG	1 cut(s)	3210
Vha464I	CTTAAG	2 cut(s)	4460, 6698
VneI	GTGCAC	1 cut(s)	1955
VpaK11BI	GGWCC	5 cut(s)	747, 2168, 5354, 5580, 5807
VspI	ATTAAT	2 cut(s)	1893, 4335
XagI	CCTNNNNNAGG	4 cut(s)	1155, 1872, 4142, 5528
XceI	RCATGY	4 cut(s)	3627, 5152, 5630, 6331
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3743
XhoI	CTCGAG	1 cut(s)	1037
XmaI	CCCGGG	1 cut(s)	1996
XmiI	GTMKAC	3 cut(s)	1196, 1321, 6242
XmnI	GAANNNNTTC	9 cut(s)	73, 805, 1005, 3526, 3611, 4240, 4528, 5222, 5655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.