Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0002990

Physical Location & Seq

Genomic Coordinates Reverse (-)

12613421 .. 12625976

Gene Structure 12556 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g14270.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6786 bp

ATGCAAAGGTATCATGCCACCGGCTGCACTGGTGCAGTTAATAACAACACAATAGGTGGGGCATCCGGAAGGGATTCTGTCCGAGCTGAATCATCTACGCTGCCAGCTCACTTACCTATAAATTCTAGGCGGCCATCTCAAATAGCACCTTACAAGTTGAAGTGTGAGAAAGACCCTTTGAATGCTCGACTTGGGCCACCTGACTTCCATCCCCAAACCCCAAATTGCCCAGAGGAGACACTTACCAGGGAATATGTCCAATCTGGATACAGGGAGACTGTTGATGGAATTGAGGAGTCCAGAGAGATATCACTTAGTCAGGTCCAAGGTTTCAGCAAGCCTCTTGTCTTCAGGTGTAGAGAGGCAATCAAAAAGCGTCTTAGAGCCATAAATGAATCTCGTGCTCAAAAGAGGAAGGCTGGTCAAGTCTATGGTGTTCCTCTTGCTGATTCACTATTAACAAAGCCTGGTGTTTTTCCGGAACAAAGGCCCTGTGGTGAAGACTTACGAAAAAAATGGATTGAGGGTTTATCACAACAACACAAACGCTTGCGTTCTTTGGCTGATCATGTTCCTCATGGGTATAGGAAAAGATCTCTATTTGAAGTTCTAACAAGGAATAATGTTCCATTGCTGAGAGCAACTTGGTTTGTTAAAGTCACTTATCTTAATCAGATCCGGCCAGGTTCTTCTAGTATATCTGGGATACCTGATAAAACTCAGTTGTCTCGCACAGAGCTCTGGACCAAAGATGTCATTGAATACTTGCAGTATCTTCTCGATGAATTCTTTTCAAGAAACAATTCACTTCTTAGTTCACACAATAGAGATCGGTCACAACAAATGCTTTATGCTGGGTCAGTATCACAACGGAGTGATCCAGCATCATCACTCCTTGATGGTGAGGAGCCTTCCCTACATTTCAAGTGGTGGTATGTGGTACGGCTTTTGCAATGGCATCATGCCGAAGGGCTGCTTCTTCCTACTCTTATCATTGAATGGGTTCTACGTCAACTGCAGGAGAAGGAATTGCTCGAGATTGTGCAGTTGCTTTTGCCTATTATATATGGTGTTCTGGAGACTGTTGTTTTATCTCAAACTTATGTGCGCAATCTTGTCGGGACAGCTGTTCGTTTCATACGCGAACCTTCCCAAGGTGGATCCGATCTTGTGGATAACTCCAGAAGGGCGTATACAGTGTCTGCTCTGGTGGAGATGCTTCGTTATTTAGTACTCTCTGTACCTGACTCGTTTGTAGCTTTGGATTGCTTTCCCTTACCACCATGTGTAGTCTCTTATGTAGCAAACGAAGGAAGTTTACCGAAGCTGTCTGATGATGTGAGAAAGATTAAAATTGGGTCAGCAGAAGTTGCTTCTGTATTTAGAAGTAAAGCTTTTGATGCTCAATTTCAATCATTGGCCTTTGATCATGTTGTTTCATCCATTCAGAAGCGTGCTGATAATCTTGAAAAGTCTACAAGCCCTAGCTATCCAAACCATAGTATTGCTAAAGCGGTACAGGCCCTAGATAGGTCTCTTGTGCAGGGGGATGTTCTTGGCGCATATAGATTTCTGTTTGAAGATCCTTGCGATGGAATTATGAATGAAAATTGGGTCGCAGAAGTTAGTCCTCGCTTGAGAACTTCATTGAAATGGATTGGGACTGTGAACCTGTCATTTATTTGTTCAGTATTTTTCCTTTGTGAGTGGGCAACTTGTGATTTCAGGGATTTTCGAACCGCTCCACCTGGTAAGTTGAAATTTACTGGCAGAAAAGATTTCTCCCAGGTGCACATTGCAGCTCGGCTTTTGTTACTAAAGATCCGAGACTTACAAAGTTCACCTCAGCATAAGAATGACAACCCTGCTAAAGGTTCCTGTCAGCAGAATAATTTCCCTGTCCGAAGTTTCATGGGAAGTTCTTATGAATCTAAAAATAAGTCAAGTGTCCACCAAAGAAGTGTGAAATCATCAAATATATTTGAGAGTCCTGGCCCTTTACATGATGTCATAGTTTGTTGGATTGATCAACATGATGTAGGAAAAGGAGAAGGTTTCAAGCGCCTCCAATTTCTTGTGATCGAACTCATACGCTCTGGTATTTTTTACCCCCATGCCTATGTTAGACAGCTGATAGTTAGTGGCATTATGGATATAAATGGGCCAGTCATTGAATCAGACAGAAGGAAGAGGCATTATCAGGTCTTGAAGTTGTTGCCTGGGCTCTTTATGCATGATGCTCTGGAAGAAGCAGGGATTGCTGAAGGCCCCAAACTTTTGGAGGCTATGTGTTCTTACTCAAATGAGCGGCGCCTCATACTTCGTGGATTTCTCGGTGATCATAATAAAAATATGAGTATGAAGTCAGCTCTGAAGCAAGAAAATAATGCCATCCCTGGAAAGGATGGTGGTTTGCCAGTTTCTGCTGATCAGTGGAAGACAGTTGAGTTGCCATCCAACATTTTGCCTGGCAAAAGTGGCAAGAGGGGCAAAAGTGATGCTGATGTTGAAGAGCTAAAGGAAGCAATCTCACTACTGTTGCAACTGCCATATAGTTCCACCCCACCAACAGATACAGGACTTGAAGAATCCCAAGGGAGTTTGAAGAGGCCTTTTGGGTTGATCTCGAACAAGATGGATTTTGGAGAAGGTACTCCTGGGTGTGAAGAATGCAGAAGAGCAAAGAGACAAAAAGTTAGTGAGGAAAGGAGCTCATATATCCAAGGAAATTCTCCAATTCCTTCAGATGATGAAGATACTTGGTGGATGAGGAAGATACCTAAATCCTCAGAGCCGCTGAAGGTTGATTTACCAGTCAAGTTAACTAAACAGGTATCTAAGAATCGGCAGAAAGGACCTCGCAAAACTCAAAGTCTTGCTCAACTAGCAGCTTCTAGGATTGAGGGGAGCCAGGGAGCTTCTACAAGTCATGTCTGCAACAATAAGATAAATTGCCCTCATCATAGATCTGGACTAGAGGGAGAAGCTCCAAAACCTACAGATACCACCAAAATGAACCATGCTGGTGATATTGTTTCGATTGGAAAAGCTTTAAAGAGGCTGCGATTTGCAGAGAAGAGGACTATTACTGTTTGGTTAATGACTAATATCAGGCAGCTTGTTGAAGAGACTGAAAAGACAATTGCTAAGGTTGGCCAGTTTGGTAGAAATTTCACAGCAGTTGATGATAGGAGCTCGACACGGTGGAAGCTTGGTGAGGACGAACTTTCTGCTGCACTGTATTTTATGGATGTTTCTGATGATTTAGTTTCAGCAGTAAAGTTTCTCCTTTGGCTGCTGCCAAAGGTTATTACTAGCCCCAATTCTACTATTCATAGTGGGAGAAACATCCTACTGCTGCCAAGAAATGTTGAAGGCCAAGTGTGTGAAGTTGGGGAGGCATTTCTAATATCATCCCTCCGAAGGTATGAGAACATACTTCTTGCAACAGATCTCATTCCAGAAGTCTTATCAGCAACAATGCACCGTGCTTCTGCAGTAGTGGCATCTAACGGAAGACTTTCAGGTTCGGCTGCCTTAGTTTACAGTCGGTATCTGTTGAAGCGGTATGGCAATGTGGCTAGTGTGATTGAATGGGAGAAGAGTTTTAAGCTATCATGTGATAAGAGGCTTTATTCTGAACTTGAAGCTGGGCAATCAGTGGATGGAGAGTTAGGGTTTCCGCTTGGTGTTCCATCTGGAGTCGAAGATCTTGATGATTATTTTCGCCAAAAGATTAGTGGTGTCCGGCCATCTCGGGTGGGTATGAACATGAGAGAGATTGTTCAAAAAAATGTGAATGTTGATGATGCCTTCCAGTATTTTTCTGGCAAGGAGAGAAAACTCTTTGCTGGCAGTACTCCTAAAGCTCCTGCAGTTGAAAAATGGGATGATGGGTATCAGATAGCTCATAAAATAATAACTGAACTGATGGACTGCATTCGGCAGACTGGTGGTGCTGCTCAAGAAGGCGACCCGACTTTGGTGTCATCTGCTGTTTCTGCTATTATCGGTAATATAGGGCCAATTCTAGCGAAAGTTCCTGATTTTAGAGCAGTGGGTTATCCATCTGCCACTGATTCCTTACACTTTGCTAGACGTATTCTGCGCATCCATATAAGTTGCCTGTGCCTTCTGAAGGAAGCTCTTGGAGAGCGTCAAACTCGTGTATTTGAGGTAGCTCTTGCCACAGAAGCTTGTTCTGCTCTTGCTGTGGCCTTTTCTCCTGGAAAGGGCTCTCGGAATCAGTCTCCTGAATCCCATGATTCCAATGAAGTTTTAAATAGCTCTTCAAAAGTAGTGATTGGGAGAGCAACAAAAGTTGCAGCTGCTGTTTCTGCTCTAATCATTGGGGCAGTTATTAATGGGATTACTAGCCTGGAGAGATTGGTGACTGTGTTCAGGTTTAAGGAAAAGTTGGATATAATTCAATTTGTGAGGAATAGCAGATCCAACTCTAATGGTAATGCTCGTTCTGCTGGGGCACTTAAGGGTGATACCTCACTTGAAGTTTATGTTCATTGGTTTAGATTGCTTGTTGGAAACTGCAGAACTGTTTCAGATGGAATGGTTGTGGAACTCTTGAGTGAACCATCCATAATAGCTCTTTCCAGGATGCAGCGAATGCTTCCTCTCCGTTTGGTTTTTCCACCTGCTTATTCAATATTTGCCTTTGTTATATGGCGGCCATTCCTTCTGAACACTAGCCTTGCAGTTCGCGAAGATATTAATCAGTTGTATCAATCTTTGGCAATAGCTGTAGGTGATATTATTAAGCATTTGCCTTTTCGTGATGTCTGTCTGAGAGATAGTCAGGGTTTTTATGATCTTGTGGCAGCAGATGGCAGTGATGCGGAATTTGCAGCCATGCTGGAGTTGAACGGTTCAGACATTCATTTAAAATCCATGGCTTTTGTTCCATTACGTGCCAGGCTTTTTCTGAATGCCCTTCTTGATTGTAAGATGCCAAACTCTTTATTCACACAGGGGGAAGGAAATCACCTATCTGGACAGGGTGAATCTAAAGTTCACTATTCAGAGCGCGAAACAAAGCTTGTAGATAAGCTTGTGCATATTTTAGATACCCTTCAACCTGCAAAATTTCATTGGCAGTGGGTAGAGCTCAGGCTCCTTTTGAATGAACAAGCCCTCATTGAAAAACTAGAGACACAGGATATGTCTTTGGTGGATGCCATACGTTCATCCTCACCTAGTCCAGAAAAGGCTGCTGCCTCTGAAAATGAGAAATATTTCATTGAAATCATCCTTACAAGATTATTGGTTAGACCTGATGCCGCTGCCCTTTTCTCAGATGTGGTTCATCTCTTTGGACGGTCACTAGCCGATTCTATGTTGTTGCAGGTTAAATGGTTCCTGGGTGGTCCAGATGTTCTTTTTGGACGAAAAACCATTAGACAACGGCTTATGAATATTGCCGAGAGTAAAGGACTTTCGACTAAGACCCACTTTTGGAAACCTTGGGGCTGGTTCACTTCTGAATTTGATATTCTGACAAACAGGGGAGATAAGAAGAAGTTTGAAGTCACTTCCCTTGAAGAGGGTGAGATGGTTGAAGAGGGGACAGAGTCAAAAAGGCATGGGAAAGGATCCAGTCCAATATTTGACAATGAAGGCCAAAGTGTCTCCCAGCAGCATGTGACAGAGAGGGCTCTCATTGAATTACTTCTTCCTTGCATAGATCAAAGTTCCGATGACTCACGGAACACTTTTGCAAATGATTTGATCAAGCAGTTGAGTAACATCGAGCAACAAATTAGTACAGTGACTCGCGGGACAAGCAAGCAAGCGGGACAAACTCCTTCTGGAATCGAAGGTCCCACGAGCAAAGGAAATAATCGCAAAGGAATACGGGGTGGCAGCCCTGGATTAGCTAGACGTGCAGCAGGAGCAGCAGATTCTGCTCCACCGTCCCCTGCAGCTTTGCGTGCTTCTATGTCGTTGAGATTGCAGTTACTCTTGAGATTGCTTCCAATCATCTATGCAGATAGGGAGCCATCAGCGCGGAACATGAGGCATGGGCTTGCCTTGGTTGTATTACGCCTTCTAGGAAACAGAGTTGTACATGAGTATCAAAGCACACCCTTAACTCCCTCGCAGAGTTCTTTATCCAAGAGGGAGTCAGATTCTTCAACAGATGCGGCCACTGCTGCTTTCACTGACTTGTCAAGCGAAAGTCTCTTTGACGAGTTATTGTTGGTTTTGCACGGGCTGTTAAGTAGTTGCCAGCCAAGTTGGTTGAGATCTACTAAGCCAACAAAAGAAAGTGGGAAAGGTTTTGTGGCATTTGATCCTGAACTGGCAGAGAATTTGCAGAATGACTTGGATCGCATGCAGCTGCCTGACTCTGTTAGGTGGCGTATTCAAACTGCAATGCCTGTAATAATTCCTTCTATTCGGTGCTTTGTGTCGTGCCACCCACCACCAGTTCCAAACATGGCTTTGGCTGTTCTTCAGCCCAGCACATCAAACTCTGGGATTTACTCCACAAACTTGAACACACCTCAGAAAAATCAGTTTCCTCTGGCACGAACAGTAACTACTGTAACAGGGAAGTCAAAGCCATTGCCATCCCAGGATAATGATATGGAGATTGACCCATGGACACTCCTGGAAGATGGTGCAGGATCAGGCCCGAGCTCATGCAACAGTGCTTTGATAGGCAGTGCAGATCATGGAAATCTTCGAGCATCTAGCTGGCTTAAGGGGGCTGTAAGAGTAAGACGGAAGGATCTTACATACATTGGTGCTGTGGATGATGACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006352 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016592 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:0070013 GO:0071704 GO:0090213 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med12

Protein Analysis

2262

Amino Acids

250.9

Weight (kDa)

8.89

Isoelectric Point (pI)

50.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med12	PF09497	159 - 219	7.7e-20	Transcription mediator complex subunit Med12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00450 AT4G00450 AT4G00450
fragaria_vesca	FvH4_7g14270 FvH4_7g14270 FvH4_7g14270
malus_domestica	MD01G1060600.v1.1 MD07G1137100.v1.1
prunus_persica	Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1 Prupe.2G170500_v2.0.a1
pyrus_communis	pycom01g09080 pycom07g13490
rosa_chinensis	RchiOBHm_Chr1g0355261
rosa_laevigata	RLG00000028166
rosa_multiflora	Rmu_sc0003115.1_g000044
rosa_roxburghii	Rroxscaffold_4G00300550
rosa_rugosa	Rorug01G0245800 Rorug01G0245900 Rorug01G0246000 Rorug01G0246100 Rorug01G0246200 Rorug01G0246200 Rorug01G0246200
rosa_samantha	Rh1AG258300 Rh1BG227900 Rh1CG242000 Rh1DG255700
rosa_wichuraiana	Rw1G022800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	4660
AasI	GACNNNNNNGTC	1 cut(s)	3985
Acc16I	TGCGCA	2 cut(s)	1109, 4109
Acc36I	ACCTGC	3 cut(s)	4660, 5101, 5350
AccB1I	GGYRCC	1 cut(s)	2310
AccBSI	CCGCTC	2 cut(s)	1742, 2308
AccI	GTMKAC	2 cut(s)	1193, 1475
AccII	CGCG	5 cut(s)	1143, 4719, 5043, 5790, 6022
AccIII	TCCGGA	2 cut(s)	65, 478
AcoI	YGGCCR	6 cut(s)	131, 680, 3154, 3749, 4685, 6159
AcsI	RAATTY	9 cut(s)	121, 785, 1760, 2728, 3169, 4856, 5099, 5498, 6325
AcuI	CTGAAG	7 cut(s)	334, 2283, 2393, 2727, 2819, 4157, 6455
AcyI	GRCGYC	1 cut(s)	2311
AdeI	CACNNNGTG	1 cut(s)	1286
AfaI	GTAC	8 cut(s)	942, 1233, 1242, 1518, 2653, 3859, 5779, 6081
AflII	CTTAAG	2 cut(s)	4487, 6719
AjiI	CACGTC	1 cut(s)	5897
Alw21I	GWGCWC	7 cut(s)	406, 741, 1794, 2714, 3197, 5124, 6659
Alw44I	GTGCAC	1 cut(s)	1790
AlwNI	CAGNNNCTG	4 cut(s)	1202, 2423, 4331, 5918
Ama87I	CYCGRG	3 cut(s)	1034, 3756, 6652
Aor13HI	TCCGGA	2 cut(s)	65, 478
ApaLI	GTGCAC	1 cut(s)	1790
ApoI	RAATTY	9 cut(s)	121, 785, 1760, 2728, 3169, 4856, 5099, 5498, 6325
AseI	ATTAAT	2 cut(s)	4362, 4728
Asp700I	GAANNNNTTC	8 cut(s)	73, 802, 1002, 1892, 3520, 4555, 5249, 5682
AspLEI	GCGC	7 cut(s)	1110, 1562, 2064, 2313, 4110, 5043, 6022
AsuII	TTCGAA	1 cut(s)	1735
AvaI	CYCGRG	3 cut(s)	1034, 3756, 6652
AvaII	GGWCC	5 cut(s)	322, 744, 2855, 5381, 5834
BaeGI	GKGCMC	2 cut(s)	1794, 4486
BaeI	ACNNNNGTAYC	2 cut(s)	6071, 6104
BalI	TGGCCA	1 cut(s)	3156
BamHI	GGATCC	2 cut(s)	1160, 5606
BanI	GGYRCC	1 cut(s)	2310
BanII	GRGCYC	8 cut(s)	741, 2226, 2714, 3197, 4238, 5124, 5671, 6659
BarI	GAAGNNNNNNTAC	6 cut(s)	1177, 1209, 1302, 1334, 6045, 6077
BauI	CACGAG	3 cut(s)	399, 4164, 5839
BbsI	GAAGAC	4 cut(s)	340, 507, 2444, 3523
Bbv12I	GWGCWC	7 cut(s)	406, 741, 1794, 2714, 3197, 5124, 6659
BbvCI	CCTCAGC	1 cut(s)	1845
BceAI	ACGGC	2 cut(s)	959, 5435
BcgI	CGANNNNNNTGC	4 cut(s)	1099, 1133, 3212, 3246
BciVI	GTATCC	2 cut(s)	260, 699
BclI	TGATCA	6 cut(s)	565, 1426, 2026, 2338, 2428, 5742
BfmI	CTRYAG	7 cut(s)	1016, 2997, 3495, 3873, 4546, 4758, 5934
BfoI	RGCGCY	2 cut(s)	2065, 2314
BfrI	CTTAAG	2 cut(s)	4487, 6719
BfuAI	ACCTGC	3 cut(s)	4660, 5101, 5350
BfuI	GTATCC	2 cut(s)	260, 699
BglII	AGATCT	5 cut(s)	593, 2966, 3451, 3709, 6260
BmcAI	AGTACT	2 cut(s)	1233, 3859
Bme18I	GGWCC	5 cut(s)	322, 744, 2855, 5381, 5834
BmeT110I	CYCGRG	3 cut(s)	1034, 3756, 6652
BmgBI	CACGTC	1 cut(s)	5897
BpiI	GAAGAC	4 cut(s)	340, 507, 2444, 3523
BpmI	CTGGAG	5 cut(s)	1097, 1165, 3720, 4400, 4892
Bpu10I	CCTNAGC	3 cut(s)	1845, 3147, 5123
Bpu14I	TTCGAA	1 cut(s)	1735
BpuEI	CTTGAG	4 cut(s)	1657, 3948, 4603, 5998
BsaAI	YACGTR	1 cut(s)	4925
BsaBI	GATNNNNATC	2 cut(s)	3897, 4728
BsaHI	GRCGYC	1 cut(s)	2311
BsaI	GGTCTC	1 cut(s)	1539
BsaWI	WCCGGW	2 cut(s)	65, 478
BsaXI	ACNNNNNCTCC	2 cut(s)	2273, 2303
Bse118I	RCCGGY	1 cut(s)	20
Bse3DI	GCAATG	6 cut(s)	629, 959, 1794, 3580, 6396, 6581
Bse8I	GATNNNNATC	2 cut(s)	3897, 4728
BseAI	TCCGGA	2 cut(s)	65, 478
BseJI	GATNNNNATC	2 cut(s)	3897, 4728
BseMI	GCAATG	6 cut(s)	629, 959, 1794, 3580, 6396, 6581
BseMII	CTCAG	8 cut(s)	626, 734, 1859, 2802, 4793, 5137, 5322, 6536
BseRI	GAGGAG	3 cut(s)	248, 308, 920
BseSI	GKGCMC	2 cut(s)	1794, 4486
BseYI	CCCAGC	5 cut(s)	854, 3650, 4478, 5646, 6476
BsgI	GTGCAG	8 cut(s)	10, 54, 1064, 1562, 3219, 5919, 6660, 6705
Bsh1236I	CGCG	5 cut(s)	1143, 4719, 5043, 5790, 6022
BshNI	GGYRCC	1 cut(s)	2310
BsiHKAI	GWGCWC	7 cut(s)	406, 741, 1794, 2714, 3197, 5124, 6659
BsiHKCI	CYCGRG	3 cut(s)	1034, 3756, 6652
BsiSI	CCGG	5 cut(s)	21, 66, 479, 679, 3748
BslFI	GGGAC	7 cut(s)	1135, 1675, 5593, 5806, 5820, 5823, 5914
BsmFI	GGGAC	7 cut(s)	1135, 1675, 5593, 5806, 5820, 5823, 5914
BsmI	GAATGC	5 cut(s)	187, 2675, 3939, 4629, 4948
Bso31I	GGTCTC	1 cut(s)	1539
BsoBI	CYCGRG	3 cut(s)	1034, 3756, 6652
Bsp119I	TTCGAA	1 cut(s)	1735
Bsp13I	TCCGGA	2 cut(s)	65, 478
Bsp1407I	TGTACA	1 cut(s)	6079
Bsp19I	CCATGG	2 cut(s)	4905, 6617
Bsp68I	TCGCGA	1 cut(s)	4719
BspCNI	CTCAG	8 cut(s)	627, 733, 1858, 2801, 4794, 5136, 5321, 6535
BspEI	TCCGGA	2 cut(s)	65, 478
BspFNI	CGCG	5 cut(s)	1143, 4719, 5043, 5790, 6022
BspMAI	CTGCAG	5 cut(s)	1020, 3499, 3877, 4550, 5938
BspMI	ACCTGC	3 cut(s)	4660, 5101, 5350
BspQI	GCTCTTC	3 cut(s)	2505, 2671, 4294
BspT104I	TTCGAA	1 cut(s)	1735
BspT107I	GGYRCC	1 cut(s)	2310
BspTI	CTTAAG	2 cut(s)	4487, 6719
BspTNI	GGTCTC	1 cut(s)	1539
BsrBI	CCGCTC	2 cut(s)	1742, 2308
BsrDI	GCAATG	6 cut(s)	629, 959, 1794, 3580, 6396, 6581
BsrFI	RCCGGY	1 cut(s)	20
BsrGI	TGTACA	1 cut(s)	6079
BssAI	RCCGGY	1 cut(s)	20
BssNAI	GTATAC	1 cut(s)	1194
BssNI	GRCGYC	1 cut(s)	2311
BssSI	CACGAG	3 cut(s)	399, 4164, 5839
BssT1I	CCWWGG	8 cut(s)	325, 1153, 2593, 2722, 4905, 5477, 6045, 6617
Bst1107I	GTATAC	1 cut(s)	1194
Bst2BI	CACGAG	3 cut(s)	399, 4164, 5839
BstACI	GRCGYC	1 cut(s)	2311
BstAFI	CTTAAG	2 cut(s)	4487, 6719
BstAPI	GCANNNNNTGC	4 cut(s)	1370, 2258, 4624, 5918
BstAUI	TGTACA	1 cut(s)	6079
BstBAI	YACGTR	1 cut(s)	4925
BstBI	TTCGAA	1 cut(s)	1735
BstDSI	CCRYGG	2 cut(s)	4905, 6617
BstENI	CCTNNNNNAGG	4 cut(s)	1152, 1869, 4136, 5555
BstFNI	CGCG	5 cut(s)	1143, 4719, 5043, 5790, 6022
BstH2I	RGCGCY	2 cut(s)	2065, 2314
BstHHI	GCGC	7 cut(s)	1110, 1562, 2064, 2313, 4110, 5043, 6022
BstNSI	RCATGY	2 cut(s)	5657, 6352
BstSFI	CTRYAG	7 cut(s)	1016, 2997, 3495, 3873, 4546, 4758, 5934
BstSLI	GKGCMC	2 cut(s)	1794, 4486
BstUI	CGCG	5 cut(s)	1143, 4719, 5043, 5790, 6022
BstV2I	GAAGAC	4 cut(s)	340, 507, 2444, 3523
BstXI	CCANNNNNNTGG	1 cut(s)	2278
BstZ17I	GTATAC	1 cut(s)	1194
BsuI	GTATCC	2 cut(s)	260, 699
BtgI	CCRYGG	2 cut(s)	4905, 6617
BtgZI	GCGATG	1 cut(s)	1605
BtrI	CACGTC	1 cut(s)	5897
BtsI	GCAGTG	5 cut(s)	4062, 4852, 5117, 6162, 6688
BtuMI	TCGCGA	1 cut(s)	4719
BveI	ACCTGC	3 cut(s)	4660, 5101, 5350
CaiI	CAGNNNCTG	4 cut(s)	1202, 2423, 4331, 5918
CfoI	GCGC	7 cut(s)	1110, 1562, 2064, 2313, 4110, 5043, 6022
Cfr10I	RCCGGY	1 cut(s)	20
CseI	GACGC	2 cut(s)	365, 4145
CsiI	ACCWGGT	1 cut(s)	1747
Csp6I	GTAC	8 cut(s)	941, 1232, 1241, 1517, 2652, 3858, 5778, 6080
CviQI	GTAC	8 cut(s)	941, 1232, 1241, 1517, 2652, 3858, 5778, 6080
DinI	GGCGCC	1 cut(s)	2312
DraI	TTTAAA	3 cut(s)	3054, 4281, 4899
DraIII	CACNNNGTG	1 cut(s)	1286
DrdI	GACNNNNNNGTC	1 cut(s)	3985
DseDI	GACNNNNNNGTC	1 cut(s)	3985
EaeI	YGGCCR	6 cut(s)	131, 680, 3154, 3749, 4685, 6159
Ecl136II	GAGCTC	5 cut(s)	739, 2712, 3195, 5122, 6657
Eco130I	CCWWGG	8 cut(s)	325, 1153, 2593, 2722, 4905, 5477, 6045, 6617
Eco147I	AGGCCT	1 cut(s)	2611
Eco24I	GRGCYC	8 cut(s)	741, 2226, 2714, 3197, 4238, 5124, 5671, 6659
Eco31I	GGTCTC	1 cut(s)	1539
Eco32I	GATATC	1 cut(s)	309
Eco47I	GGWCC	5 cut(s)	322, 744, 2855, 5381, 5834
Eco53kI	GAGCTC	5 cut(s)	739, 2712, 3195, 5122, 6657
Eco57I	CTGAAG	7 cut(s)	334, 2283, 2393, 2727, 2819, 4157, 6455
Eco88I	CYCGRG	3 cut(s)	1034, 3756, 6652
EcoICRI	GAGCTC	5 cut(s)	739, 2712, 3195, 5122, 6657
EcoNI	CCTNNNNNAGG	4 cut(s)	1152, 1869, 4136, 5555
EcoO109I	RGGNCCY	5 cut(s)	489, 1522, 2267, 2855, 5834
EcoRI	GAATTC	1 cut(s)	785
EcoRV	GATATC	1 cut(s)	309
EcoT14I	CCWWGG	8 cut(s)	325, 1153, 2593, 2722, 4905, 5477, 6045, 6617
EcoT22I	ATGCAT	1 cut(s)	2235
EcoT38I	GRGCYC	8 cut(s)	741, 2226, 2714, 3197, 4238, 5124, 5671, 6659
EgeI	GGCGCC	1 cut(s)	2312
EheI	GGCGCC	1 cut(s)	2312
ErhI	CCWWGG	8 cut(s)	325, 1153, 2593, 2722, 4905, 5477, 6045, 6617
FalI	AAGNNNNNCTT	6 cut(s)	960, 992, 1378, 1410, 5450, 5482
FaqI	GGGAC	7 cut(s)	1135, 1675, 5593, 5806, 5820, 5823, 5914
FauI	CCCGC	2 cut(s)	5783, 5800
FbaI	TGATCA	6 cut(s)	565, 1426, 2026, 2338, 2428, 5742
FblI	GTMKAC	2 cut(s)	1193, 1475
FriOI	GRGCYC	8 cut(s)	741, 2226, 2714, 3197, 4238, 5124, 5671, 6659
FspI	TGCGCA	2 cut(s)	1109, 4109
GlaI	GCGC	7 cut(s)	1109, 1561, 2063, 2312, 4109, 5042, 6021
GsaI	CCCAGC	5 cut(s)	858, 3654, 4482, 5650, 6480
GsuI	CTGGAG	5 cut(s)	1097, 1165, 3720, 4400, 4892
HaeII	RGCGCY	2 cut(s)	2065, 2314
HapII	CCGG	5 cut(s)	21, 66, 479, 679, 3748
HgaI	GACGC	2 cut(s)	365, 4145
HhaI	GCGC	7 cut(s)	1110, 1562, 2064, 2313, 4110, 5043, 6022
Hin1I	GRCGYC	1 cut(s)	2311
Hin6I	GCGC	7 cut(s)	1108, 1560, 2062, 2311, 4108, 5041, 6020
HinP1I	GCGC	7 cut(s)	1108, 1560, 2062, 2311, 4108, 5041, 6020
HincII	GTYRAC	2 cut(s)	1013, 2823
HindII	GTYRAC	2 cut(s)	1013, 2823
HindIII	AAGCTT	6 cut(s)	1392, 3048, 3209, 4194, 5051, 5063
HpaI	GTTAAC	1 cut(s)	2823
HpaII	CCGG	5 cut(s)	21, 66, 479, 679, 3748
HpyCH4IV	ACGT	5 cut(s)	1009, 4099, 4924, 5197, 5896
HpySE526I	ACGT	5 cut(s)	1009, 4099, 4924, 5197, 5896
Hsp92I	GRCGYC	1 cut(s)	2311
HspAI	GCGC	7 cut(s)	1108, 1560, 2062, 2311, 4108, 5041, 6020
KasI	GGCGCC	1 cut(s)	2310
Kpn2I	TCCGGA	2 cut(s)	65, 478
Ksp22I	TGATCA	6 cut(s)	565, 1426, 2026, 2338, 2428, 5742
KspAI	GTTAAC	1 cut(s)	2823
LguI	GCTCTTC	3 cut(s)	2505, 2671, 4294
MabI	ACCWGGT	1 cut(s)	1747
MaeII	ACGT	5 cut(s)	1009, 4099, 4924, 5197, 5896
MbiI	CCGCTC	2 cut(s)	1742, 2308
MfeI	CAATTG	1 cut(s)	3141
MlsI	TGGCCA	1 cut(s)	3156
MluNI	TGGCCA	1 cut(s)	3156
Mly113I	GGCGCC	1 cut(s)	2311
MlyI	GAGTC	9 cut(s)	305, 1241, 1996, 3711, 5594, 5708, 5779, 6146, 6356
MmeI	TCCRAC	5 cut(s)	2000, 2481, 4398, 4476, 4519
Mox20I	TGGCCA	1 cut(s)	3156
Mph1103I	ATGCAT	1 cut(s)	2235
MroI	TCCGGA	2 cut(s)	65, 478
MroXI	GAANNNNTTC	8 cut(s)	73, 802, 1002, 1892, 3520, 4555, 5249, 5682
MscI	TGGCCA	1 cut(s)	3156
MslI	CAYNNNNRTG	5 cut(s)	1651, 1854, 2118, 3011, 3024
Msp20I	TGGCCA	1 cut(s)	3156
MspA1I	CMGCKG	7 cut(s)	1127, 2131, 2797, 4328, 5297, 6355, 6783
MspCI	CTTAAG	2 cut(s)	4487, 6719
MspI	CCGG	5 cut(s)	21, 66, 479, 679, 3748
MunI	CAATTG	1 cut(s)	3141
Mva1269I	GAATGC	5 cut(s)	187, 2675, 3939, 4629, 4948
MvnI	CGCG	5 cut(s)	1143, 4719, 5043, 5790, 6022
NarI	GGCGCC	1 cut(s)	2311
NcoI	CCATGG	2 cut(s)	4905, 6617
NmeAIII	GCCGAG	2 cut(s)	1783, 5461
NmuCI	GTSAC	7 cut(s)	658, 834, 4390, 5334, 5542, 5656, 5782
NruI	TCGCGA	1 cut(s)	4719
NsbI	TGCGCA	2 cut(s)	1109, 4109
NsiI	ATGCAT	1 cut(s)	2235
NspI	RCATGY	2 cut(s)	5657, 6352
NspV	TTCGAA	1 cut(s)	1735
PaeI	GCATGC	1 cut(s)	6352
PaeR7I	CTCGAG	1 cut(s)	1034
PaqCI	CACCTGC	1 cut(s)	4660
PceI	AGGCCT	1 cut(s)	2611
PciSI	GCTCTTC	3 cut(s)	2505, 2671, 4294
PctI	GAATGC	5 cut(s)	187, 2675, 3939, 4629, 4948
PdmI	GAANNNNTTC	8 cut(s)	73, 802, 1002, 1892, 3520, 4555, 5249, 5682
PflFI	GACNNNGTC	1 cut(s)	5584
PfoI	TCCNGGA	3 cut(s)	4225, 4610, 6627
PleI	GAGTC	9 cut(s)	304, 1241, 1995, 3710, 5593, 5708, 5779, 6145, 6356
PluTI	GGCGCC	1 cut(s)	2314
PpsI	GAGTC	9 cut(s)	304, 1241, 1995, 3710, 5593, 5708, 5779, 6145, 6356
Ppu21I	YACGTR	1 cut(s)	4925
PpuMI	RGGWCCY	2 cut(s)	2855, 5834
PshBI	ATTAAT	2 cut(s)	4362, 4728
Psp124BI	GAGCTC	5 cut(s)	741, 2714, 3197, 5124, 6659
Psp5II	RGGWCCY	2 cut(s)	2855, 5834
PspFI	CCCAGC	5 cut(s)	854, 3650, 4478, 5646, 6476
PspPPI	RGGWCCY	2 cut(s)	2855, 5834
PsrI	GAACNNNNNNTAC	4 cut(s)	4456, 4488, 6541, 6573
PstI	CTGCAG	5 cut(s)	1020, 3499, 3877, 4550, 5938
PstNI	CAGNNNCTG	4 cut(s)	1202, 2423, 4331, 5918
PsyI	GACNNNGTC	1 cut(s)	5584
PvuII	CAGCTG	5 cut(s)	1127, 2131, 4328, 6355, 6783
RruI	TCGCGA	1 cut(s)	4719
RsaI	GTAC	8 cut(s)	942, 1233, 1242, 1518, 2653, 3859, 5779, 6081
RsaNI	GTAC	8 cut(s)	941, 1232, 1241, 1517, 2652, 3858, 5778, 6080
RseI	CAYNNNNRTG	5 cut(s)	1651, 1854, 2118, 3011, 3024
SacI	GAGCTC	5 cut(s)	741, 2714, 3197, 5124, 6659
SapI	GCTCTTC	3 cut(s)	2505, 2671, 4294
ScaI	AGTACT	2 cut(s)	1233, 3859
SchI	GAGTC	9 cut(s)	305, 1241, 1996, 3711, 5594, 5708, 5779, 6146, 6356
SexAI	ACCWGGT	1 cut(s)	1747
SfcI	CTRYAG	7 cut(s)	1016, 2997, 3495, 3873, 4546, 4758, 5934
SfoI	GGCGCC	1 cut(s)	2312
Sfr274I	CTCGAG	1 cut(s)	1034
SfuI	TTCGAA	1 cut(s)	1735
SinI	GGWCC	5 cut(s)	322, 744, 2855, 5381, 5834
SlaI	CTCGAG	1 cut(s)	1034
SmiMI	CAYNNNNRTG	5 cut(s)	1651, 1854, 2118, 3011, 3024
SmlI	CTYRAG	7 cut(s)	1034, 1636, 3963, 4487, 4582, 5977, 6719
SmoI	CTYRAG	7 cut(s)	1034, 1636, 3963, 4487, 4582, 5977, 6719
SphI	GCATGC	1 cut(s)	6352
SseBI	AGGCCT	1 cut(s)	2611
SspDI	GGCGCC	1 cut(s)	2310
SspI	AATATT	4 cut(s)	4665, 5249, 5431, 5619
SstI	GAGCTC	5 cut(s)	741, 2714, 3197, 5124, 6659
StuI	AGGCCT	1 cut(s)	2611
StyI	CCWWGG	8 cut(s)	325, 1153, 2593, 2722, 4905, 5477, 6045, 6617
TaiI	ACGT	5 cut(s)	1012, 4102, 4927, 5200, 5899
TaqII	GACCGA	1 cut(s)	822
TatI	WGTACW	4 cut(s)	1231, 3857, 5777, 6079
TauI	GCSGC	6 cut(s)	133, 2311, 2797, 4687, 5297, 6161
TseFI	GTSAC	7 cut(s)	658, 834, 4390, 5334, 5542, 5656, 5782
Tsp45I	GTSAC	7 cut(s)	658, 834, 4390, 5334, 5542, 5656, 5782
TspGWI	ACGGA	5 cut(s)	886, 3528, 4625, 5734, 6757
Tth111I	GACNNNGTC	1 cut(s)	5584
Vha464I	CTTAAG	2 cut(s)	4487, 6719
VneI	GTGCAC	1 cut(s)	1790
VpaK11BI	GGWCC	5 cut(s)	322, 744, 2855, 5381, 5834
VspI	ATTAAT	2 cut(s)	4362, 4728
XagI	CCTNNNNNAGG	4 cut(s)	1152, 1869, 4136, 5555
XapI	RAATTY	9 cut(s)	121, 785, 1760, 2728, 3169, 4856, 5099, 5498, 6325
XceI	RCATGY	2 cut(s)	5657, 6352
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3737, 3824
XhoI	CTCGAG	1 cut(s)	1034
XmiI	GTMKAC	2 cut(s)	1193, 1475
XmnI	GAANNNNTTC	8 cut(s)	73, 802, 1002, 1892, 3520, 4555, 5249, 5682
ZrmI	AGTACT	2 cut(s)	1233, 3859
Zsp2I	ATGCAT	1 cut(s)	2235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.