AT4G05018

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014210
Physical Location & Seq
Forward (+)
2571040 .. 2572208
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05018.1

Sequence Viewer

Length: 708 bp
ATGGAGGAAACTGGTAAAATCCCATGGACGATCTTCTTCTCTCATCCTCTGTTCTCTTGTTTCATTTCACTCTACACTATATTTCTTCTTTATTTCCCTCAAGATTCTCTTCGAATTCTTATTTCCCATGTCCCTCTCCTCATCGGAGCTTTCTTAATCTCCTGCCTCCATCTCGGGTCGACCCGGAAATCCATTGCCCGACCCGGAAGCAATGAGGATTCGATAGGACCTGAACTCAAAACGGGTCTCGGTTTAAACCCGGATTTTATGGAATGGAACTTTAAATTGGGAGCTCCATTGGAAGTTATTCACAAAGAAGAAGAAGAACAGTCGATGACGGGTGTAAAAGATCCGACCCGGTTCGTGATGATCAAGAGGTTTCCATCGTTATCGATGTTTAACCCGGAATCTGATTCCGAATTGGACCGCGATTTCCCGGTTATGAGTGAAAATGATCCAACCCGGTTCGTGAGGCTGGAAAGGTCAATTAAACCGGAATCTGATATGGATTACGACTCCGAATTGGACAACAATTTGGTTATTGATGTAAAAGATCGAACTCGGTTCGTGAAGGTGGAGATATTCCCATCGTTGTCAATCTTTAAACCGGATTCGAATTCCGGTTCGCCGGAGAAATTGCGTTGTATATACGAAATCAAAACGGAAACGCTTTTTGTAGTTTTATATTCCTGGAGAACAATATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

235

Amino Acids

27.13

Weight (kDa)

5.05

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05018 AT4G21500
fragaria_vesca FvH4_3g03970
malus_domestica MD05G1325600.v1.1 MD10G1303500.v1.1
prunus_persica Prupe.4G037000_v2.0.a1
pyrus_communis pycom05g29970
rosa_chinensis RchiOBHm_Chr5g0006301
rosa_laevigata RLG00000031366
rosa_multiflora Rmu_co8467785.1_g000001
rosa_roxburghii Rroxscaffold_2G00115670
rosa_rugosa Rorug04G0420900
rosa_samantha Rh5AG048800 Rh5BG047800 Rh5CG056200 Rh5DG047100
rosa_wichuraiana Rw5G004860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 179
AccII CGCG 1 cut(s) 429
AciI CCGC 1 cut(s) 427
AclWI GGATC 2 cut(s) 344, 449
AcsI RAATTY 2 cut(s) 114, 616
AjnI CCWGG 1 cut(s) 689
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AluBI AGCT 2 cut(s) 149, 293
AluI AGCT 2 cut(s) 149, 293
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 2 cut(s) 344, 449
Ama87I CYCGRG 1 cut(s) 173
ApoI RAATTY 2 cut(s) 114, 616
Asp700I GAANNNNTTC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 227, 424
AsuC2I CCSGG 7 cut(s) 184, 204, 260, 358, 404, 437, 463
AsuII TTCGAA 2 cut(s) 112, 614
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 2 cut(s) 227, 424
BanII GRGCYC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 295
BccI CCATC 3 cut(s) 177, 391, 595
BciT130I CCWGG 1 cut(s) 691
BclI TGATCA 1 cut(s) 369
BcnI CCSGG 7 cut(s) 184, 204, 260, 358, 404, 437, 463
BcoDI GTCTC 1 cut(s) 251
Bme1390I CCNGG 8 cut(s) 184, 204, 260, 358, 404, 437, 463, 691
Bme18I GGWCC 2 cut(s) 227, 424
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 227, 424
BmrFI CCNGG 8 cut(s) 184, 204, 260, 358, 404, 437, 463, 691
Bpu14I TTCGAA 2 cut(s) 112, 614
BpuEI CTTGAG 1 cut(s) 84
BpuMI CCSGG 7 cut(s) 184, 204, 260, 358, 404, 437, 463
Bsa29I ATCGAT 1 cut(s) 392
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 23
BsaWI WCCGGW 3 cut(s) 493, 607, 620
Bse1I ACTGG 1 cut(s) 16
Bse3DI GCAATG 2 cut(s) 192, 217
BseBI CCWGG 1 cut(s) 691
BseCI ATCGAT 1 cut(s) 392
BseDI CCNNGG 1 cut(s) 23
BseGI GGATG 1 cut(s) 43
BseMI GCAATG 2 cut(s) 192, 217
BseNI ACTGG 1 cut(s) 16
BseRI GAGGAG 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 429
BshVI ATCGAT 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 116
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 173
Bsp119I TTCGAA 2 cut(s) 112, 614
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 5 cut(s) 30, 349, 369, 454, 553
Bsp19I CCATGG 1 cut(s) 23
BspACI CCGC 1 cut(s) 427
BspDI ATCGAT 1 cut(s) 392
BspFNI CGCG 1 cut(s) 429
BspPI GGATC 2 cut(s) 344, 449
BspT104I TTCGAA 2 cut(s) 112, 614
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 2 cut(s) 192, 217
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 23
BssMI GATC 5 cut(s) 30, 349, 369, 454, 553
BssT1I CCWWGG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 691
Bst4CI ACNGT 1 cut(s) 330
Bst6I CTCTTC 1 cut(s) 114
BstBI TTCGAA 2 cut(s) 112, 614
BstDSI CCRYGG 1 cut(s) 23
BstF5I GGATG 1 cut(s) 43
BstFNI CGCG 1 cut(s) 429
BstKTI GATC 5 cut(s) 33, 352, 372, 457, 556
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 5 cut(s) 30, 349, 369, 454, 553
BstNI CCWGG 1 cut(s) 691
BstSCI CCNGG 8 cut(s) 182, 202, 258, 356, 402, 435, 461, 689
BstUI CGCG 1 cut(s) 429
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 392
BsuTUI ATCGAT 1 cut(s) 392
BtgI CCRYGG 1 cut(s) 23
BtsCI GGATG 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 227, 424
ClaI ATCGAT 1 cut(s) 392
CviAII CATG 2 cut(s) 24, 128
CviJI RGCY 3 cut(s) 149, 293, 475
CviKI_1 RGCY 3 cut(s) 149, 293, 475
DpnI GATC 5 cut(s) 32, 351, 371, 456, 555
DpnII GATC 5 cut(s) 30, 349, 369, 454, 553
DraI TTTAAA 3 cut(s) 255, 283, 604
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Ecl136II GAGCTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 2 cut(s) 227, 424
Eco53kI GAGCTC 1 cut(s) 293
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 227
EcoRI GAATTC 2 cut(s) 114, 616
EcoRII CCWGG 1 cut(s) 689
EcoT14I CCWWGG 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 2 cut(s) 27, 131
FaiI YATR 9 cut(s) 25, 80, 129, 269, 443, 506, 647, 649, 685
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 23, 127
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 1 cut(s) 179
FokI GGATG 1 cut(s) 30
FriOI GRGCYC 1 cut(s) 295
Hin1II CATG 2 cut(s) 27, 131
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 7 cut(s) 104, 218, 407, 413, 497, 515, 611
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 6 cut(s) 146, 354, 412, 418, 502, 520
Hpy188III TCNNGA 5 cut(s) 101, 364, 373, 469, 568
Hpy8I GTNNAC 1 cut(s) 180
HpyAV CCTTC 1 cut(s) 565
HpyCH4III ACNGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 27, 131
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 5 cut(s) 30, 349, 369, 454, 553
LmnI GCTCC 3 cut(s) 146, 290, 298
MalI GATC 5 cut(s) 32, 351, 371, 456, 555
MboI GATC 5 cut(s) 30, 349, 369, 454, 553
MboII GAAGA 7 cut(s) 25, 28, 77, 101, 329, 332, 335
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 8 cut(s) 114, 284, 419, 486, 521, 532, 616, 635
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 2 cut(s) 377, 482
MnlI CCTC 8 cut(s) 57, 108, 144, 149, 176, 208, 369, 465
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 6 cut(s) 155, 254, 282, 399, 489, 603
MspR9I CCNGG 8 cut(s) 184, 204, 260, 358, 404, 437, 463, 691
MssI GTTTAAAC 1 cut(s) 255
MvaI CCWGG 1 cut(s) 691
MvnI CGCG 1 cut(s) 429
NciI CCSGG 7 cut(s) 184, 204, 260, 358, 404, 437, 463
NcoI CCATGG 1 cut(s) 23
NdeII GATC 5 cut(s) 30, 349, 369, 454, 553
NlaIII CATG 2 cut(s) 27, 131
NspV TTCGAA 2 cut(s) 112, 614
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 6 cut(s) 104, 218, 407, 413, 497, 611
PfoI TCCNGGA 1 cut(s) 689
PleI GAGTC 1 cut(s) 509
PmeI GTTTAAAC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 509
PpuMI RGGWCCY 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 689
PspGI CCWGG 1 cut(s) 689
PspPI GGNCC 2 cut(s) 227, 424
PspPPI RGGWCCY 1 cut(s) 227
PsuI RGATCY 1 cut(s) 349
SacI GAGCTC 1 cut(s) 295
SalI GTCGAC 1 cut(s) 178
SaqAI TTAA 6 cut(s) 155, 254, 282, 399, 489, 603
Sau3AI GATC 5 cut(s) 30, 349, 369, 454, 553
Sau96I GGNCC 2 cut(s) 227, 424
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 8 cut(s) 184, 204, 260, 358, 404, 437, 463, 691
SduI GDGCHC 1 cut(s) 295
SetI ASST 6 cut(s) 151, 232, 295, 380, 485, 576
SfuI TTCGAA 2 cut(s) 112, 614
SinI GGWCC 2 cut(s) 227, 424
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
Sse9I AATT 8 cut(s) 114, 284, 419, 486, 521, 532, 616, 635
SsiI CCGC 1 cut(s) 427
SspI AATATT 1 cut(s) 702
SstI GAGCTC 1 cut(s) 295
StyD4I CCNGG 8 cut(s) 182, 202, 258, 356, 402, 435, 461, 689
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 1 cut(s) 330
TaqI TCGA 7 cut(s) 112, 179, 221, 332, 392, 556, 614
TasI AATT 8 cut(s) 114, 284, 419, 486, 521, 532, 616, 635
TfiI GAWTC 6 cut(s) 104, 218, 407, 413, 497, 611
Tru1I TTAA 6 cut(s) 155, 254, 282, 399, 489, 603
Tru9I TTAA 6 cut(s) 155, 254, 282, 399, 489, 603
TspDTI ATGAA 1 cut(s) 52
TspGWI ACGGA 1 cut(s) 677
VpaK11BI GGWCC 2 cut(s) 227, 424
XapI RAATTY 2 cut(s) 114, 616
XmiI GTMKAC 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.