MD10G1303500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014210
Physical Location & Seq
Forward (+)
38996349 .. 38997245
897 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1303500.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGAAGAAAATGCGAGTCTCATCATCTGGGAATTCAATATCTTAACTTCCATTCCAAACTTGCAAATTTCTGAAACCATGACGAACTTTTGCGCTTCGATCTCCTCAAGTCCTCTGTTTTCCAGCATTGTCGCTCTCTATGCTCTGATCCTCCTCTACCTCCCGAACCATTTCCTCCGAATCGTTTTATCACCGGTCCTGATCATCACCGGAATTCTCCTACTCACGATTCTCCGTATCGGCGCGATTCAGAGGATTGAAACGGAAGACGATGAACGCAAACAAATGGAAACCAATCGCTCTCTGCTCGAAGGCGGAGAAAACAGGGGAAGCAGCAATGGAGTCCAAGAACAAATCCCAAGTTTGCAAGGCGACCAAGAACACAGATTTTTCGACAACCAATCCGAAACAGACTCCGAAAGCGAAGCGGGTTTCGACCCGAACCCGTGTTTCGAAGACTTGTTCGTTGAGTGGAACCTGAAGGCGCCATTGGAGGTCATTTATGAAGAGTACGAAGGTGAAGAAAATGAGGTGGATCCGAATGAAAACGACCCGGATTCGAATCGAATGGAAGGAAACCAAGTTCTGGGTCTTGATAAGCACCCTTCGTTGTCGCTGTATTACCCGGAGTCGGACTCGGATAGCTCTTCGGACGGCCGGTTTTCGGTGACTGGAGTGTGGGACTCGCCGGAGGCGATGTGTTTCCGGTGGGAGGAGGATAGAGAAGGGTTGATAGAGATTGCTCTGGACGGCAATTCAAAGCGAGGCAGCTTGGATTTTCGGGTTGATCACGATCACGAGGAAGAAAATTTGATAGAGATTGATATATCTCCGACGAGAAGCAATGAATTTTGCGCCGAGAAATGGCAGTTCTCCGGCGATCTTAGGTTCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

33.89

Weight (kDa)

4.16

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05018 AT4G21500
fragaria_vesca FvH4_3g03970
malus_domestica MD05G1325600.v1.1 MD10G1303500.v1.1
prunus_persica Prupe.4G037000_v2.0.a1
pyrus_communis pycom05g29970
rosa_chinensis RchiOBHm_Chr5g0006301
rosa_laevigata RLG00000031366
rosa_multiflora Rmu_co8467785.1_g000001
rosa_roxburghii Rroxscaffold_2G00115670
rosa_rugosa Rorug04G0420900
rosa_samantha Rh5AG048800 Rh5BG047800 Rh5CG056200 Rh5DG047100
rosa_wichuraiana Rw5G004860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 484
AccB7I CCANNNNNTGG 1 cut(s) 586
AccII CGCG 1 cut(s) 245
AciI CCGC 2 cut(s) 315, 428
AclWI GGATC 3 cut(s) 142, 530, 543
AcoI YGGCCR 1 cut(s) 655
AcsI RAATTY 5 cut(s) 32, 66, 213, 808, 848
AcuI CTGAAG 1 cut(s) 500
AcyI GRCGYC 1 cut(s) 485
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 5 cut(s) 586, 631, 664, 712, 864
AgeI ACCGGT 1 cut(s) 193
AgsI TTSAA 3 cut(s) 37, 260, 759
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AloI GAACNNNNNNTCC 2 cut(s) 567, 599
AluBI AGCT 3 cut(s) 645, 771, 894
AluI AGCT 3 cut(s) 645, 771, 894
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 3 cut(s) 142, 530, 543
AoxI GGCC 1 cut(s) 655
ApeKI GCWGC 2 cut(s) 333, 768
ApoI RAATTY 5 cut(s) 32, 66, 213, 808, 848
AsiGI ACCGGT 1 cut(s) 193
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 4 cut(s) 95, 245, 487, 857
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 2 cut(s) 554, 626
AsuHPI GGTGA 4 cut(s) 183, 199, 530, 679
AsuII TTCGAA 2 cut(s) 453, 560
AvaII GGWCC 1 cut(s) 196
BamHI GGATCC 1 cut(s) 535
BanI GGYRCC 1 cut(s) 484
BauI CACGAG 1 cut(s) 797
BbsI GAAGAC 2 cut(s) 273, 462
BbvI GCAGC 2 cut(s) 345, 780
BceAI ACGGC 2 cut(s) 670, 766
BclI TGATCA 2 cut(s) 201, 787
BcnI CCSGG 2 cut(s) 554, 626
BcoDI GTCTC 1 cut(s) 23
BfoI RGCGCY 1 cut(s) 488
BisI GCNGC 2 cut(s) 334, 769
BlsI GCNGC 2 cut(s) 335, 770
Bme1390I CCNGG 2 cut(s) 554, 626
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 3 cut(s) 476, 486, 537
BmrFI CCNGG 2 cut(s) 554, 626
BpiI GAAGAC 2 cut(s) 273, 462
BplI GAGNNNNNCTC 2 cut(s) 620, 652
BpmI CTGGAG 1 cut(s) 693
Bpu14I TTCGAA 2 cut(s) 453, 560
BpuEI CTTGAG 1 cut(s) 91
BpuMI CCSGG 2 cut(s) 554, 626
BsaBI GATNNNNATC 2 cut(s) 561, 792
BsaHI GRCGYC 1 cut(s) 485
BsaWI WCCGGW 3 cut(s) 193, 209, 705
BsaXI ACNNNNNCTCC 2 cut(s) 683, 713
Bsc4I CCNNNNNNNGG 5 cut(s) 586, 631, 664, 712, 864
Bse118I RCCGGY 2 cut(s) 193, 657
Bse1I ACTGG 1 cut(s) 676
Bse3DI GCAATG 2 cut(s) 343, 850
Bse8I GATNNNNATC 2 cut(s) 561, 792
BseJI GATNNNNATC 2 cut(s) 561, 792
BseLI CCNNNNNNNGG 5 cut(s) 586, 631, 664, 712, 864
BseMI GCAATG 2 cut(s) 343, 850
BseNI ACTGG 1 cut(s) 676
BseRI GAGGAG 3 cut(s) 94, 143, 728
BseX3I CGGCCG 1 cut(s) 655
BseXI GCAGC 2 cut(s) 345, 780
Bsh1236I CGCG 1 cut(s) 245
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 1 cut(s) 657
BshNI GGYRCC 1 cut(s) 484
BshTI ACCGGT 1 cut(s) 193
BsiEI CGRYCG 1 cut(s) 658
BsiSI CCGG 8 cut(s) 194, 210, 554, 626, 658, 689, 706, 876
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 5 cut(s) 586, 631, 664, 712, 864
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 695
BsnI GGCC 1 cut(s) 657
Bsp119I TTCGAA 2 cut(s) 453, 560
Bsp143I GATC 7 cut(s) 99, 147, 201, 535, 787, 793, 880
BspACI CCGC 2 cut(s) 315, 428
BspANI GGCC 1 cut(s) 657
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 3 cut(s) 476, 486, 537
BspPI GGATC 3 cut(s) 142, 530, 543
BspQI GCTCTTC 1 cut(s) 652
BspT104I TTCGAA 2 cut(s) 453, 560
BspT107I GGYRCC 1 cut(s) 484
BsrDI GCAATG 2 cut(s) 343, 850
BsrFI RCCGGY 2 cut(s) 193, 657
BsrI ACTGG 1 cut(s) 676
BssAI RCCGGY 2 cut(s) 193, 657
BssMI GATC 7 cut(s) 99, 147, 201, 535, 787, 793, 880
BssNI GRCGYC 1 cut(s) 485
BssSI CACGAG 1 cut(s) 797
Bst2BI CACGAG 1 cut(s) 797
Bst6I CTCTTC 2 cut(s) 501, 652
BstACI GRCGYC 1 cut(s) 485
BstBI TTCGAA 2 cut(s) 453, 560
BstDEI CTNAG 1 cut(s) 884
BstFNI CGCG 1 cut(s) 245
BstH2I RGCGCY 1 cut(s) 488
BstHHI GCGC 4 cut(s) 95, 245, 487, 857
BstKTI GATC 7 cut(s) 102, 150, 204, 538, 790, 796, 883
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 7 cut(s) 99, 147, 201, 535, 787, 793, 880
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 552, 624
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 2 cut(s) 345, 780
BstV2I GAAGAC 2 cut(s) 273, 462
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BstZI CGGCCG 1 cut(s) 655
BsuRI GGCC 1 cut(s) 657
BtgZI GCGATG 1 cut(s) 710
CfoI GCGC 4 cut(s) 95, 245, 487, 857
Cfr10I RCCGGY 2 cut(s) 193, 657
Cfr13I GGNCC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 511
CspAI ACCGGT 1 cut(s) 193
CviAII CATG 1 cut(s) 79
CviJI RGCY 4 cut(s) 645, 657, 771, 894
CviKI_1 RGCY 4 cut(s) 645, 657, 771, 894
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 1 cut(s) 884
DinI GGCGCC 1 cut(s) 486
DpnI GATC 7 cut(s) 101, 149, 203, 537, 789, 795, 882
DpnII GATC 7 cut(s) 99, 147, 201, 535, 787, 793, 880
EaeI YGGCCR 1 cut(s) 655
EagI CGGCCG 1 cut(s) 655
Eam1104I CTCTTC 2 cut(s) 501, 652
EarI CTCTTC 2 cut(s) 501, 652
EciI GGCGGA 1 cut(s) 330
EclXI CGGCCG 1 cut(s) 655
Eco47I GGWCC 1 cut(s) 196
Eco52I CGGCCG 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 500
EcoRI GAATTC 2 cut(s) 32, 213
EgeI GGCGCC 1 cut(s) 486
EheI GGCGCC 1 cut(s) 486
FaeI CATG 1 cut(s) 82
FaiI YATR 4 cut(s) 80, 141, 504, 827
FaqI GGGAC 1 cut(s) 695
FatI CATG 1 cut(s) 78
FauI CCCGC 1 cut(s) 421
FbaI TGATCA 2 cut(s) 201, 787
Fnu4HI GCNGC 2 cut(s) 334, 769
Fsp4HI GCNGC 2 cut(s) 334, 769
GlaI GCGC 4 cut(s) 94, 244, 486, 856
GluI GCNGC 2 cut(s) 334, 769
GsuI CTGGAG 1 cut(s) 693
HaeII RGCGCY 1 cut(s) 488
HaeIII GGCC 1 cut(s) 657
HapII CCGG 8 cut(s) 194, 210, 554, 626, 658, 689, 706, 876
HhaI GCGC 4 cut(s) 95, 245, 487, 857
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 4 cut(s) 93, 243, 485, 855
HinP1I GCGC 4 cut(s) 93, 243, 485, 855
HpaII CCGG 8 cut(s) 194, 210, 554, 626, 658, 689, 706, 876
HphI GGTGA 4 cut(s) 183, 199, 530, 679
Hpy188III TCNNGA 7 cut(s) 163, 199, 226, 593, 746, 791, 797
Hpy99I CGWCG 1 cut(s) 838
HpyAV CCTTC 6 cut(s) 305, 475, 509, 566, 615, 719
HpyCH4V TGCA 2 cut(s) 64, 367
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 884
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 4 cut(s) 93, 243, 485, 855
KasI GGCGCC 1 cut(s) 484
Ksp22I TGATCA 2 cut(s) 201, 787
Kzo9I GATC 7 cut(s) 99, 147, 201, 535, 787, 793, 880
LguI GCTCTTC 1 cut(s) 652
Lsp1109I GCAGC 2 cut(s) 345, 780
MaeIII GTNAC 1 cut(s) 667
MalI GATC 7 cut(s) 101, 149, 203, 537, 789, 795, 882
MboI GATC 7 cut(s) 99, 147, 201, 535, 787, 793, 880
MboII GAAGA 7 cut(s) 17, 278, 467, 518, 533, 639, 815
MflI RGATCY 1 cut(s) 535
MluCI AATT 6 cut(s) 32, 66, 213, 754, 808, 848
Mly113I GGCGCC 1 cut(s) 485
MlyI GAGTC 6 cut(s) 25, 351, 407, 629, 638, 677
MmeI TCCRAC 2 cut(s) 612, 857
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 44
MspI CCGG 8 cut(s) 194, 210, 554, 626, 658, 689, 706, 876
MspR9I CCNGG 2 cut(s) 554, 626
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 1 cut(s) 140
NarI GGCGCC 1 cut(s) 485
NciI CCSGG 2 cut(s) 554, 626
NdeII GATC 7 cut(s) 99, 147, 201, 535, 787, 793, 880
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 3 cut(s) 476, 486, 537
NmeAIII GCCGAG 1 cut(s) 883
NmuCI GTSAC 1 cut(s) 667
NspV TTCGAA 2 cut(s) 453, 560
PciSI GCTCTTC 1 cut(s) 652
PcsI WCGNNNNNNNCGW 1 cut(s) 692
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 5 cut(s) 180, 229, 247, 557, 562
PflMI CCANNNNNTGG 1 cut(s) 586
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 2 cut(s) 335, 770
PleI GAGTC 6 cut(s) 24, 350, 407, 629, 637, 677
PluTI GGCGCC 1 cut(s) 488
PpsI GAGTC 6 cut(s) 24, 350, 407, 629, 637, 677
PspN4I GGNNCC 3 cut(s) 476, 486, 537
PspPI GGNCC 1 cut(s) 196
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SapI GCTCTTC 1 cut(s) 652
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 2 cut(s) 334, 769
Sau3AI GATC 7 cut(s) 99, 147, 201, 535, 787, 793, 880
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 6 cut(s) 25, 351, 407, 629, 638, 677
ScrFI CCNGG 2 cut(s) 554, 626
SetI ASST 9 cut(s) 162, 480, 498, 520, 534, 647, 773, 890, 896
SfoI GGCGCC 1 cut(s) 486
SfuI TTCGAA 2 cut(s) 453, 560
SinI GGWCC 1 cut(s) 196
SmlI CTYRAG 1 cut(s) 106
SmoI CTYRAG 1 cut(s) 106
Sse9I AATT 6 cut(s) 32, 66, 213, 754, 808, 848
SsiI CCGC 2 cut(s) 315, 428
SspDI GGCGCC 1 cut(s) 484
StyD4I CCNGG 2 cut(s) 552, 624
TaqI TCGA 7 cut(s) 98, 309, 393, 435, 453, 560, 565
TasI AATT 6 cut(s) 32, 66, 213, 754, 808, 848
TfiI GAWTC 5 cut(s) 180, 229, 247, 557, 562
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 2 cut(s) 333, 768
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 4 cut(s) 288, 519, 558, 861
TspGWI ACGGA 2 cut(s) 224, 278
Van91I CCANNNNNTGG 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 5 cut(s) 32, 66, 213, 808, 848
XmnI GAANNNNTTC 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.