AT4G21500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014210
Physical Location & Seq
Reverse (-)
11440878 .. 11441903
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21500.1

Sequence Viewer

Length: 648 bp
ATGGAAGAAGAAACTCTACAAGTCTTCTTCTTCTCTCATCCTCTCTTCTCCATTTTCATAACTCTCTACAGTCTAATCCTCATCTATTTCCCCAATGATTTGCTTCGAATCTTTCTCTCCCCTGTTCTTCTCATCTCCGGAGCTCTCCTTCTCTCTCTTCTCCGTCTCGGATCGACCCGAGAACCTAACACCCAACCCGATAAAAGCTACGAGGAGTCTGAAGAACCTAAGGTTTTTCTGGTTCGTGAATCCGATTTGATGGGTGGTTTTGTGGAATGGAACTTGAGAGCACCATTGGAAGTGATTCACGAAGCTTACGAAGATGAAGAAGAGGAAGAAGAAGAAGAAGATCCGACCCGGTTTAGAAAAATGGAGAGATTCCCATCGTTGTCACTCTGTTACCCAGAATCAGATTCGGAATCGGATTCTGCTTCCTCGTCAGAATTCAATTTCCCGGAAATCGGTGACTGGAACTCGCCGGAAAATATGGGATTCAGATGGGAAGAAGACGAGGGTGGAATAGGATGTGAAGGTTTGATTGAGATAAAGCTCGATCATTATAGCCATAATAGGAAGATGATGAGTAAAACAGAGATTGATTTTCATGCAGAGGAAGATGGTTTGATCGAGATCGATCTTTTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

215

Amino Acids

25.01

Weight (kDa)

4.22

Isoelectric Point (pI)

80.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05018 AT4G21500
fragaria_vesca FvH4_3g03970
malus_domestica MD05G1325600.v1.1 MD10G1303500.v1.1
prunus_persica Prupe.4G037000_v2.0.a1
pyrus_communis pycom05g29970
rosa_chinensis RchiOBHm_Chr5g0006301
rosa_laevigata RLG00000031366
rosa_multiflora Rmu_co8467785.1_g000001
rosa_roxburghii Rroxscaffold_2G00115670
rosa_rugosa Rorug04G0420900
rosa_samantha Rh5AG048800 Rh5BG047800 Rh5CG056200 Rh5DG047100
rosa_wichuraiana Rw5G004860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 137
AclWI GGATC 2 cut(s) 178, 344
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 240
AfiI CCNNNNNNNGG 1 cut(s) 461
AgsI TTSAA 1 cut(s) 448
AluBI AGCT 4 cut(s) 143, 207, 314, 550
AluI AGCT 4 cut(s) 143, 207, 314, 550
Alw21I GWGCWC 2 cut(s) 145, 292
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 2 cut(s) 178, 344
Ama87I CYCGRG 1 cut(s) 177
Aor13HI TCCGGA 1 cut(s) 137
ApoI RAATTY 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 303
AsuC2I CCSGG 2 cut(s) 358, 455
AsuHPI GGTGA 1 cut(s) 476
AsuII TTCGAA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 177
AxyI CCTNAGG 1 cut(s) 228
BanII GRGCYC 1 cut(s) 145
BarI GAAGNNNNNNTAC 1 cut(s) 32
BbsI GAAGAC 2 cut(s) 16, 513
Bbv12I GWGCWC 2 cut(s) 145, 292
BccI CCATC 4 cut(s) 253, 391, 492, 611
BcnI CCSGG 2 cut(s) 358, 455
BcoDI GTCTC 1 cut(s) 170
BfmI CTRYAG 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 358, 455
BmeT110I CYCGRG 1 cut(s) 177
BmrFI CCNGG 2 cut(s) 358, 455
BpiI GAAGAC 2 cut(s) 16, 513
Bpu14I TTCGAA 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 304
BpuMI CCSGG 2 cut(s) 358, 455
Bsa29I ATCGAT 1 cut(s) 633
BsaBI GATNNNNATC 2 cut(s) 382, 629
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse1I ACTGG 1 cut(s) 473
Bse21I CCTNAGG 1 cut(s) 228
Bse8I GATNNNNATC 2 cut(s) 382, 629
BseAI TCCGGA 1 cut(s) 137
BseCI ATCGAT 1 cut(s) 633
BseGI GGATG 2 cut(s) 37, 530
BseJI GATNNNNATC 2 cut(s) 382, 629
BseLI CCNNNNNNNGG 1 cut(s) 461
BseNI ACTGG 1 cut(s) 473
BseRI GAGGAG 1 cut(s) 227
BshVI ATCGAT 1 cut(s) 633
BsiHKAI GWGCWC 2 cut(s) 145, 292
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 4 cut(s) 138, 358, 455, 479
BslI CCNNNNNNNGG 1 cut(s) 461
BsmAI GTCTC 1 cut(s) 170
BsmBI CGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 177
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 145, 292
Bsp13I TCCGGA 1 cut(s) 137
Bsp143I GATC 6 cut(s) 170, 349, 553, 624, 630, 634
BspDI ATCGAT 1 cut(s) 633
BspEI TCCGGA 1 cut(s) 137
BspPI GGATC 2 cut(s) 178, 344
BspT104I TTCGAA 1 cut(s) 106
BsrI ACTGG 1 cut(s) 473
BssMI GATC 6 cut(s) 170, 349, 553, 624, 630, 634
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 3 cut(s) 50, 162, 324
BstBI TTCGAA 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 228
BstF5I GGATG 2 cut(s) 37, 530
BstKTI GATC 6 cut(s) 173, 352, 556, 627, 633, 637
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 6 cut(s) 170, 349, 553, 624, 630, 634
BstSCI CCNGG 2 cut(s) 356, 453
BstSFI CTRYAG 1 cut(s) 67
BstV2I GAAGAC 2 cut(s) 16, 513
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 633
Bsu36I CCTNAGG 1 cut(s) 228
BsuTUI ATCGAT 1 cut(s) 633
BtsCI GGATG 2 cut(s) 37, 530
ClaI ATCGAT 1 cut(s) 633
CviAII CATG 2 cut(s) 605, 645
CviJI RGCY 5 cut(s) 143, 207, 314, 550, 564
CviKI_1 RGCY 5 cut(s) 143, 207, 314, 550, 564
DdeI CTNAG 1 cut(s) 228
DpnI GATC 6 cut(s) 172, 351, 555, 626, 632, 636
DpnII GATC 6 cut(s) 170, 349, 553, 624, 630, 634
Eam1104I CTCTTC 3 cut(s) 50, 162, 324
EarI CTCTTC 3 cut(s) 50, 162, 324
Ecl136II GAGCTC 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 240
Eco81I CCTNAGG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 177
EcoICRI GAGCTC 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 443
EcoT38I GRGCYC 1 cut(s) 145
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 2 cut(s) 608, 648
FaiI YATR 6 cut(s) 59, 488, 561, 567, 606, 646
FatI CATG 2 cut(s) 604, 644
FokI GGATG 2 cut(s) 24, 537
FriOI GRGCYC 1 cut(s) 145
HapII CCGG 4 cut(s) 138, 358, 455, 479
Hin1II CATG 2 cut(s) 608, 648
HindIII AAGCTT 1 cut(s) 312
HpaII CCGG 4 cut(s) 138, 358, 455, 479
HphI GGTGA 1 cut(s) 476
Hpy188I TCNGA 9 cut(s) 170, 220, 253, 354, 412, 418, 424, 442, 497
Hpy188III TCNNGA 4 cut(s) 138, 245, 308, 628
HpyAV CCTTC 2 cut(s) 158, 524
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 1 cut(s) 608
HpyF3I CTNAG 1 cut(s) 228
Hsp92II CATG 2 cut(s) 608, 648
Kpn2I TCCGGA 1 cut(s) 137
Kzo9I GATC 6 cut(s) 170, 349, 553, 624, 630, 634
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 8 cut(s) 135, 151, 224, 371, 417, 454, 468, 492
MaeIII GTNAC 3 cut(s) 390, 398, 464
MalI GATC 6 cut(s) 172, 351, 555, 626, 632, 636
MboI GATC 6 cut(s) 170, 349, 553, 624, 630, 634
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 2 cut(s) 145, 292
MluCI AATT 2 cut(s) 443, 448
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 7 cut(s) 51, 89, 205, 325, 445, 505, 604
MroI TCCGGA 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 303
MspI CCGG 4 cut(s) 138, 358, 455, 479
MspR9I CCNGG 2 cut(s) 358, 455
NciI CCSGG 2 cut(s) 358, 455
NdeII GATC 6 cut(s) 170, 349, 553, 624, 630, 634
NlaIII CATG 2 cut(s) 608, 648
NmuCI GTSAC 2 cut(s) 390, 464
NspV TTCGAA 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 9 cut(s) 108, 248, 304, 378, 407, 413, 419, 425, 492
PfoI TCCNGGA 1 cut(s) 453
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp124BI GAGCTC 1 cut(s) 145
PsuI RGATCY 1 cut(s) 349
SacI GAGCTC 1 cut(s) 145
Sau3AI GATC 6 cut(s) 170, 349, 553, 624, 630, 634
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 358, 455
SduI GDGCHC 2 cut(s) 145, 292
SetI ASST 8 cut(s) 145, 187, 209, 229, 234, 316, 535, 552
SfcI CTRYAG 1 cut(s) 67
SfuI TTCGAA 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 283
SmoI CTYRAG 1 cut(s) 283
Sse9I AATT 2 cut(s) 443, 448
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 2 cut(s) 356, 453
TaaI ACNGT 1 cut(s) 71
TaqI TCGA 5 cut(s) 106, 173, 552, 627, 633
TasI AATT 2 cut(s) 443, 448
TfiI GAWTC 9 cut(s) 108, 248, 304, 378, 407, 413, 419, 425, 492
TseFI GTSAC 2 cut(s) 390, 464
Tsp45I GTSAC 2 cut(s) 390, 464
TspDTI ATGAA 3 cut(s) 46, 339, 593
TspGWI ACGGA 1 cut(s) 152
XapI RAATTY 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.