MD05G1325600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014210
Physical Location & Seq
Forward (+)
45167175 .. 45168023
849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1325600.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGAAGAAAATGCGAGTCACATCGTCTGGGAATCCAATTCCAAAACTTCCATTCCAAACTTACTACTTTCCGAAACCATGACCACCTTTTGTGCTTCAATTTCCTCAAGCCCTCTGTTTTCCAGCATTGTCGCTCTCTGCGCTCTGATCCTCCTCTACTTCCCCAACCATTTCCTCCAAATCATTTTTTCGCCGGTCCTGATCATCACCGGATTCCTCATCCTCTCGATTCTCCGCCTCGGCGCAGTTCAGAGAATCGAAACGGAAGGCGATGAGCTAAAAGAAAAGGAAACCAGTCGCTCTCTGCTCGAAAGCGAAGAAAACAGGGGAAACTGCAATGGAATCCAAGAAAAAATCCCAAGTTTGGAAGAGGACCAAGATCAAAGATTTTTAGACTACCAATCTGAAACAGACTCCGAAAGTGAAGCGGGTTTCGACCCGAAGCCGTGCTTCGAAGACTTGTTCGTCGAGTGGAACGTGAAGGCGCCACTGGAGGTCATTTATGAAGAGTACGAAGGTGAAGAAGATGAGGTGGATCCGAATGGAAACGATCCGGACTCAAATCGAAAGGAGGGAAACCAAGTTCTGAGTCTCGAGAAGCACCCTTCGTTGTCGCTGTGTTACCCGGAGTCGGACTCGGATAGCTCGTCGGATTGTGGGTTTTCGGTACCCGGAGCGTGGGACTCGCCGGATGCGATGTGTTTCCGGTGGGAGGATGATAGAGAAGGGCTTATAGAGATTGCTCTGGACAGCAATTCAAAGAGAGGCGAATTGGATTTTCGGTTTGATCACGATCACGATGAAGAGAATTTGATAGAGATTGATATATCTCCGACGAGAAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

31.92

Weight (kDa)

4.16

Isoelectric Point (pI)

57.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05018 AT4G21500
fragaria_vesca FvH4_3g03970
malus_domestica MD05G1325600.v1.1 MD10G1303500.v1.1
prunus_persica Prupe.4G037000_v2.0.a1
pyrus_communis pycom05g29970
rosa_chinensis RchiOBHm_Chr5g0006301
rosa_laevigata RLG00000031366
rosa_multiflora Rmu_co8467785.1_g000001
rosa_roxburghii Rroxscaffold_2G00115670
rosa_rugosa Rorug04G0420900
rosa_samantha Rh5AG048800 Rh5BG047800 Rh5CG056200 Rh5DG047100
rosa_wichuraiana Rw5G004860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 464
Acc65I GGTACC 1 cut(s) 667
AccB1I GGYRCC 2 cut(s) 484, 667
AccIII TCCGGA 1 cut(s) 553
AciI CCGC 2 cut(s) 235, 428
AclWI GGATC 4 cut(s) 142, 530, 543, 545
AcsI RAATTY 1 cut(s) 808
AcyI GRCGYC 1 cut(s) 485
AfaI GTAC 2 cut(s) 512, 669
AfiI CCNNNNNNNGG 4 cut(s) 364, 631, 678, 712
AgsI TTSAA 2 cut(s) 99, 759
AloI GAACNNNNNNTCC 2 cut(s) 567, 599
AluBI AGCT 2 cut(s) 277, 645
AluI AGCT 2 cut(s) 277, 645
Alw26I GTCTC 1 cut(s) 596
AlwI GGATC 4 cut(s) 142, 530, 543, 545
Ama87I CYCGRG 1 cut(s) 593
Aor13HI TCCGGA 1 cut(s) 553
ApoI RAATTY 1 cut(s) 808
Asp718I GGTACC 1 cut(s) 667
AspLEI GCGC 3 cut(s) 143, 245, 487
AspS9I GGNCC 2 cut(s) 196, 373
AsuC2I CCSGG 2 cut(s) 626, 672
AsuHPI GGTGA 2 cut(s) 199, 530
AsuII TTCGAA 1 cut(s) 453
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 2 cut(s) 196, 373
BamHI GGATCC 1 cut(s) 535
BanI GGYRCC 2 cut(s) 484, 667
BbsI GAAGAC 1 cut(s) 462
BceAI ACGGC 1 cut(s) 430
BclI TGATCA 2 cut(s) 201, 787
BcnI CCSGG 2 cut(s) 626, 672
BcoDI GTCTC 1 cut(s) 596
BfoI RGCGCY 1 cut(s) 488
Bme1390I CCNGG 2 cut(s) 626, 672
Bme18I GGWCC 2 cut(s) 196, 373
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 2 cut(s) 196, 373
BmiI GGNNCC 3 cut(s) 486, 537, 669
BmrFI CCNGG 2 cut(s) 626, 672
BmsI GCATC 1 cut(s) 682
BpiI GAAGAC 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 620, 652
BpmI CTGGAG 1 cut(s) 512
Bpu14I TTCGAA 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 91
BpuMI CCSGG 2 cut(s) 626, 672
BsaBI GATNNNNATC 1 cut(s) 792
BsaHI GRCGYC 1 cut(s) 485
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 3 cut(s) 209, 553, 705
Bsc4I CCNNNNNNNGG 4 cut(s) 364, 631, 678, 712
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 294, 495
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 792
BseAI TCCGGA 1 cut(s) 553
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 3 cut(s) 219, 697, 721
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 4 cut(s) 364, 631, 678, 712
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 578
BseNI ACTGG 2 cut(s) 294, 495
BseRI GAGGAG 1 cut(s) 143
BshNI GGYRCC 2 cut(s) 484, 667
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 7 cut(s) 194, 210, 554, 626, 672, 689, 706
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 4 cut(s) 364, 631, 678, 712
BsmAI GTCTC 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 695
BsoBI CYCGRG 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 453
Bsp13I TCCGGA 1 cut(s) 553
Bsp143I GATC 7 cut(s) 147, 201, 379, 535, 550, 787, 793
BspACI CCGC 2 cut(s) 235, 428
BspCNI CTCAG 1 cut(s) 579
BspEI TCCGGA 1 cut(s) 553
BspLI GGNNCC 3 cut(s) 486, 537, 669
BspPI GGATC 4 cut(s) 142, 530, 543, 545
BspT104I TTCGAA 1 cut(s) 453
BspT107I GGYRCC 2 cut(s) 484, 667
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 2 cut(s) 294, 495
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 7 cut(s) 147, 201, 379, 535, 550, 787, 793
BssNI GRCGYC 1 cut(s) 485
Bst6I CTCTTC 3 cut(s) 363, 501, 798
BstACI GRCGYC 1 cut(s) 485
BstBI TTCGAA 1 cut(s) 453
BstDEI CTNAG 1 cut(s) 587
BstF5I GGATG 3 cut(s) 219, 697, 721
BstH2I RGCGCY 1 cut(s) 488
BstHHI GCGC 3 cut(s) 143, 245, 487
BstKTI GATC 7 cut(s) 150, 204, 382, 538, 553, 790, 796
BstMAI GTCTC 1 cut(s) 596
BstMBI GATC 7 cut(s) 147, 201, 379, 535, 550, 787, 793
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 624, 670
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BtgZI GCGATG 2 cut(s) 285, 710
BtsCI GGATG 3 cut(s) 219, 697, 721
BtsIMutI CAGTG 1 cut(s) 488
CfoI GCGC 3 cut(s) 143, 245, 487
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 196, 373
Csp6I GTAC 2 cut(s) 511, 668
CviAII CATG 1 cut(s) 79
CviJI RGCY 5 cut(s) 111, 277, 445, 645, 730
CviKI_1 RGCY 5 cut(s) 111, 277, 445, 645, 730
CviQI GTAC 2 cut(s) 511, 668
DdeI CTNAG 1 cut(s) 587
DinI GGCGCC 1 cut(s) 486
DpnI GATC 7 cut(s) 149, 203, 381, 537, 552, 789, 795
DpnII GATC 7 cut(s) 147, 201, 379, 535, 550, 787, 793
DrdI GACNNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 464
Eam1104I CTCTTC 3 cut(s) 363, 501, 798
EarI CTCTTC 3 cut(s) 363, 501, 798
EciI GGCGGA 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 196, 373
Eco88I CYCGRG 1 cut(s) 593
EgeI GGCGCC 1 cut(s) 486
EheI GGCGCC 1 cut(s) 486
FaeI CATG 1 cut(s) 82
FaiI YATR 4 cut(s) 80, 504, 734, 827
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 1 cut(s) 695
FatI CATG 1 cut(s) 78
FauI CCCGC 1 cut(s) 421
FbaI TGATCA 2 cut(s) 201, 787
FokI GGATG 3 cut(s) 206, 704, 728
GlaI GCGC 3 cut(s) 142, 244, 486
GsuI CTGGAG 1 cut(s) 512
HaeII RGCGCY 1 cut(s) 488
HapII CCGG 7 cut(s) 194, 210, 554, 626, 672, 689, 706
HhaI GCGC 3 cut(s) 143, 245, 487
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 3 cut(s) 141, 243, 485
HinP1I GCGC 3 cut(s) 141, 243, 485
HpaII CCGG 7 cut(s) 194, 210, 554, 626, 672, 689, 706
HphI GGTGA 2 cut(s) 199, 530
Hpy188III TCNNGA 8 cut(s) 199, 226, 554, 593, 595, 746, 791, 797
Hpy99I CGWCG 3 cut(s) 470, 652, 838
HpyAV CCTTC 5 cut(s) 260, 475, 509, 615, 719
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 1 cut(s) 336
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 587
HpySE526I ACGT 1 cut(s) 477
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 3 cut(s) 141, 243, 485
KasI GGCGCC 1 cut(s) 484
Kpn2I TCCGGA 1 cut(s) 553
KpnI GGTACC 1 cut(s) 671
Ksp22I TGATCA 2 cut(s) 201, 787
Kzo9I GATC 7 cut(s) 147, 201, 379, 535, 550, 787, 793
LmnI GCTCC 1 cut(s) 674
LweI GCATC 1 cut(s) 682
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 2 cut(s) 17, 620
MalI GATC 7 cut(s) 149, 203, 381, 537, 552, 789, 795
MboI GATC 7 cut(s) 147, 201, 379, 535, 550, 787, 793
MboII GAAGA 8 cut(s) 17, 329, 380, 467, 518, 533, 536, 815
MflI RGATCY 1 cut(s) 535
MluCI AATT 5 cut(s) 37, 99, 754, 770, 808
Mly113I GGCGCC 1 cut(s) 485
MlyI GAGTC 7 cut(s) 25, 407, 551, 598, 629, 638, 677
MmeI TCCRAC 2 cut(s) 612, 630
MroI TCCGGA 1 cut(s) 553
MspI CCGG 7 cut(s) 194, 210, 554, 626, 672, 689, 706
MspR9I CCNGG 2 cut(s) 626, 672
MwoI GCNNNNNNNGC 1 cut(s) 140
NarI GGCGCC 1 cut(s) 485
NciI CCSGG 2 cut(s) 626, 672
NdeII GATC 7 cut(s) 147, 201, 379, 535, 550, 787, 793
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 3 cut(s) 486, 537, 669
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 17
NspV TTCGAA 1 cut(s) 453
PaeR7I CTCGAG 1 cut(s) 593
PcsI WCGNNNNNNNCGW 3 cut(s) 474, 644, 692
PfeI GAWTC 5 cut(s) 32, 213, 229, 255, 342
PleI GAGTC 7 cut(s) 24, 407, 551, 597, 629, 637, 677
PluTI GGCGCC 1 cut(s) 488
PpsI GAGTC 7 cut(s) 24, 407, 551, 597, 629, 637, 677
PspN4I GGNNCC 3 cut(s) 486, 537, 669
PspPI GGNCC 2 cut(s) 196, 373
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 2 cut(s) 512, 669
RsaNI GTAC 2 cut(s) 511, 668
Sau3AI GATC 7 cut(s) 147, 201, 379, 535, 550, 787, 793
Sau96I GGNCC 2 cut(s) 196, 373
SchI GAGTC 7 cut(s) 25, 407, 551, 598, 629, 638, 677
ScrFI CCNGG 2 cut(s) 626, 672
SetI ASST 7 cut(s) 89, 279, 480, 498, 520, 534, 647
SfaNI GCATC 1 cut(s) 682
SfoI GGCGCC 1 cut(s) 486
Sfr274I CTCGAG 1 cut(s) 593
SfuI TTCGAA 1 cut(s) 453
SinI GGWCC 2 cut(s) 196, 373
SlaI CTCGAG 1 cut(s) 593
SmlI CTYRAG 2 cut(s) 106, 593
SmoI CTYRAG 2 cut(s) 106, 593
Sse9I AATT 5 cut(s) 37, 99, 754, 770, 808
SsiI CCGC 2 cut(s) 235, 428
SspDI GGCGCC 1 cut(s) 484
StyD4I CCNGG 2 cut(s) 624, 670
TaiI ACGT 1 cut(s) 480
TaqI TCGA 8 cut(s) 227, 258, 309, 435, 453, 468, 565, 594
TasI AATT 5 cut(s) 37, 99, 754, 770, 808
TfiI GAWTC 5 cut(s) 32, 213, 229, 255, 342
TscAI CASTG 1 cut(s) 495
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 2 cut(s) 519, 816
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 1 cut(s) 495
VpaK11BI GGWCC 2 cut(s) 196, 373
XapI RAATTY 1 cut(s) 808
XhoI CTCGAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.