AT4G06744

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014329
Physical Location & Seq
Reverse (-)
4070549 .. 4072470
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G06744.1

Sequence Viewer

Length: 1215 bp
ATGATGAATCATTTGCTTTTGGCTCTTTTCCTTGTTGGAACCATCGCCTTACCAGATAGAGGAACTCAACAATTGACCAACGATGGCCTCACCGGCGCGAGGCGAGAGATACTCTCTTTCTTAGACCAAAGACTTGCAGTGGTGTACCCAGTGATCCAGAGATTCAAGTCACTCATCACTCTCGACCCTTATAACGTGACCAAGACATGGATTGGTTCAGACATCTGCAGTTATAGAGGCTTCCACTGTGACAATCCTCCTCACAACAAAACCGCAGTTACTGTTGCTTCCATCGATTTCAATGGCTTTCAGCTCTCTGCTCCATCCATCGAAGGATTTATCGATCAATTCGCTGACCTAGCTCTGTTTCATGTTAACTCAAACAATTTTGGAGGTACGGTGCCTTCCAAGATAGTCAACCTCCGGTATCTCTATGAACTCGACATCAGCAACAATAGATTCACCGGTCAGTTTCCGACAGCTGTTGTTGGAATGTCCGGTTTGACGTTTATTGACATCCGGTTCAACTCTTTCTCTGGTTCAATCCCACCACAAATCCTCGGTCAGAACCTCGAAGTTCTCTTCATCAATGACAACGGCTTCACTGCGAGCCTCCCGGAGATACCTGGTGATGGTACAACACACATTCTCTTCTTGACTTTAGCAAACAACAAGTTCAACGGTCCTCTCCCAAGAAGCATCTTAAGATCAATGTCAACTTTAACCGAAGTACTCTTCCTCAATAACGATTTTACGGGTTGTATCCCTCACGAGATCGGGTTCCTCACCGGAGCATCAGTGATCGATATCGGTGGAAACAAGCTCACGGGTCCTTTGCCGCTTTCCCTAATGTGTTTAGAGAAAGTAGAGCAGCTCAACTTTGCTGGGAATCTATTGTTTGGTGCCGTCCCGGAGGCAGTATGCATGCTGCTCCGGGACAATCTTGTCAACTTGTCGTTGTCGGATAACTATTTCACGCACGTAGGGCCTTGGTGTAGGGGTTTACTTGAAAAAGGTGTTCTTGACGTTGGTAACAATTGCATTCCTTTCTTCCCAGGACAAAGATCGATGCAAGAATGTGCGGAGTTCTTTGTAAAACCTAAGAAGTACTACTGTCCTCACATGTGGTTCCATAGTTTTTTCTCTTGTAGATATTCTCACATATCTTCTTCCTCTTCTAGTGCTTTCATGCCCATGGTGGCTCCCTCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

404

Amino Acids

44.62

Weight (kDa)

6.05

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 192
AasI GACNNNNNNGTC 1 cut(s) 944
AccB1I GGYRCC 2 cut(s) 400, 902
AccB7I CCANNNNNTGG 1 cut(s) 207
AccII CGCG 1 cut(s) 98
AciI CCGC 3 cut(s) 273, 839, 1082
AclWI GGATC 1 cut(s) 148
AfaI GTAC 5 cut(s) 146, 397, 637, 732, 1109
AfiI CCNNNNNNNGG 4 cut(s) 59, 99, 207, 632
AflII CTTAAG 1 cut(s) 703
AflIII ACRYGT 1 cut(s) 1122
AgeI ACCGGT 1 cut(s) 464
AgsI TTSAA 6 cut(s) 166, 301, 526, 543, 679, 1010
AjnI CCWGG 2 cut(s) 625, 1054
AluBI AGCT 5 cut(s) 313, 362, 482, 823, 874
AluI AGCT 5 cut(s) 313, 362, 482, 823, 874
AlwI GGATC 1 cut(s) 148
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 2 cut(s) 85, 986
ApeKI GCWGC 2 cut(s) 871, 928
ArsI GACNNNNNNTTYG 2 cut(s) 547, 579
AsiGI ACCGGT 1 cut(s) 464
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 3 cut(s) 683, 830, 986
AsuC2I CCSGG 3 cut(s) 617, 911, 935
AsuHPI GGTGA 4 cut(s) 82, 454, 641, 778
AvaII GGWCC 2 cut(s) 683, 830
BanI GGYRCC 2 cut(s) 400, 902
BarI GAAGNNNNNNTAC 2 cut(s) 388, 420
BauI CACGAG 1 cut(s) 770
BbvI GCAGC 2 cut(s) 883, 915
BccI CCATC 6 cut(s) 50, 77, 299, 331, 335, 626
BceAI ACGGC 2 cut(s) 613, 890
BciT130I CCWGG 2 cut(s) 627, 1056
BciVI GTATCC 1 cut(s) 773
BcnI CCSGG 3 cut(s) 617, 911, 935
BfaI CTAG 2 cut(s) 359, 1179
BfmI CTRYAG 1 cut(s) 226
BfrI CTTAAG 1 cut(s) 703
BfuI GTATCC 1 cut(s) 773
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 3 cut(s) 839, 872, 929
BlsI GCNGC 3 cut(s) 840, 873, 930
BmcAI AGTACT 2 cut(s) 732, 1109
Bme1390I CCNGG 5 cut(s) 617, 627, 911, 935, 1056
Bme18I GGWCC 2 cut(s) 683, 830
BmgT120I GGNCC 3 cut(s) 683, 830, 986
BmiI GGNNCC 7 cut(s) 40, 402, 782, 831, 904, 1130, 1203
BmrFI CCNGG 5 cut(s) 617, 627, 911, 935, 1056
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 3 cut(s) 708, 803, 1059
BmuI ACTGGG 1 cut(s) 143
BplI GAGNNNNNCTC 4 cut(s) 96, 128, 98, 130
BpuMI CCSGG 3 cut(s) 617, 911, 935
Bsa29I ATCGAT 4 cut(s) 294, 342, 804, 1067
BsaAI YACGTR 1 cut(s) 982
BsaBI GATNNNNATC 1 cut(s) 806
BsaJI CCNNGG 4 cut(s) 559, 989, 1054, 1194
BsaWI WCCGGW 5 cut(s) 423, 464, 497, 519, 788
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 783, 813
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 99, 207, 632
Bse118I RCCGGY 2 cut(s) 92, 464
Bse1I ACTGG 1 cut(s) 149
Bse8I GATNNNNATC 1 cut(s) 806
BseBI CCWGG 2 cut(s) 627, 1056
BseCI ATCGAT 4 cut(s) 294, 342, 804, 1067
BseDI CCNNGG 4 cut(s) 559, 989, 1054, 1194
BseGI GGATG 2 cut(s) 323, 516
BseJI GATNNNNATC 1 cut(s) 806
BseLI CCNNNNNNNGG 4 cut(s) 59, 99, 207, 632
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 1 cut(s) 249
BseXI GCAGC 2 cut(s) 883, 915
BseYI CCCAGC 1 cut(s) 884
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 2 cut(s) 87, 988
BshNI GGYRCC 2 cut(s) 400, 902
BshTI ACCGGT 1 cut(s) 464
BshVI ATCGAT 4 cut(s) 294, 342, 804, 1067
BsiSI CCGG 9 cut(s) 93, 424, 465, 498, 520, 617, 789, 911, 934
BslFI GGGAC 2 cut(s) 893, 950
BslI CCNNNNNNNGG 4 cut(s) 59, 99, 207, 632
BsmFI GGGAC 2 cut(s) 893, 950
BsmI GAATGC 1 cut(s) 1041
BsnI GGCC 2 cut(s) 87, 988
Bsp143I GATC 6 cut(s) 153, 343, 707, 774, 801, 1064
Bsp19I CCATGG 1 cut(s) 1194
BspACI CCGC 3 cut(s) 273, 839, 1082
BspANI GGCC 2 cut(s) 87, 988
BspDI ATCGAT 4 cut(s) 294, 342, 804, 1067
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 7 cut(s) 40, 402, 782, 831, 904, 1130, 1203
BspMAI CTGCAG 1 cut(s) 230
BspPI GGATC 1 cut(s) 148
BspT107I GGYRCC 2 cut(s) 400, 902
BspTI CTTAAG 1 cut(s) 703
BsrFI RCCGGY 2 cut(s) 92, 464
BsrI ACTGG 1 cut(s) 149
BssAI RCCGGY 2 cut(s) 92, 464
BssECI CCNNGG 4 cut(s) 559, 989, 1054, 1194
BssMI GATC 6 cut(s) 153, 343, 707, 774, 801, 1064
BssSI CACGAG 1 cut(s) 770
BssT1I CCWWGG 2 cut(s) 989, 1194
Bst2BI CACGAG 1 cut(s) 770
Bst2UI CCWGG 2 cut(s) 627, 1056
Bst4CI ACNGT 5 cut(s) 248, 283, 400, 683, 1115
Bst6I CTCTTC 4 cut(s) 587, 656, 740, 1180
BstAFI CTTAAG 1 cut(s) 703
BstBAI YACGTR 1 cut(s) 982
BstC8I GCNNGC 2 cut(s) 610, 926
BstDEI CTNAG 2 cut(s) 121, 1101
BstDSI CCRYGG 1 cut(s) 1194
BstF5I GGATG 2 cut(s) 323, 516
BstFNI CGCG 1 cut(s) 98
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 6 cut(s) 156, 346, 710, 777, 804, 1067
BstMBI GATC 6 cut(s) 153, 343, 707, 774, 801, 1064
BstMWI GCNNNNNNNGC 3 cut(s) 93, 359, 985
BstNI CCWGG 2 cut(s) 627, 1056
BstNSI RCATGY 2 cut(s) 928, 1126
BstSCI CCNGG 5 cut(s) 615, 625, 909, 933, 1054
BstSFI CTRYAG 1 cut(s) 226
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 883, 915
Bsu15I ATCGAT 4 cut(s) 294, 342, 804, 1067
BsuI GTATCC 1 cut(s) 773
BsuRI GGCC 2 cut(s) 87, 988
BsuTUI ATCGAT 4 cut(s) 294, 342, 804, 1067
BtgI CCRYGG 1 cut(s) 1194
BtgZI GCGATG 1 cut(s) 28
BtsCI GGATG 2 cut(s) 323, 516
BtsI GCAGTG 2 cut(s) 144, 603
BtsIMutI CAGTG 5 cut(s) 144, 156, 244, 603, 804
Cac8I GCNNGC 2 cut(s) 610, 926
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 1 cut(s) 98
Cfr10I RCCGGY 2 cut(s) 92, 464
Cfr13I GGNCC 3 cut(s) 683, 830, 986
ClaI ATCGAT 4 cut(s) 294, 342, 804, 1067
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 5 cut(s) 145, 396, 636, 731, 1108
CspAI ACCGGT 1 cut(s) 464
CviAII CATG 6 cut(s) 207, 371, 925, 1123, 1189, 1195
CviQI GTAC 5 cut(s) 145, 396, 636, 731, 1108
DdeI CTNAG 2 cut(s) 121, 1101
DpnI GATC 6 cut(s) 155, 345, 709, 776, 803, 1066
DpnII GATC 6 cut(s) 153, 343, 707, 774, 801, 1064
DrdI GACNNNNNNGTC 1 cut(s) 944
DseDI GACNNNNNNGTC 1 cut(s) 944
Eam1104I CTCTTC 4 cut(s) 587, 656, 740, 1180
EarI CTCTTC 4 cut(s) 587, 656, 740, 1180
Eco130I CCWWGG 2 cut(s) 989, 1194
Eco32I GATATC 1 cut(s) 808
Eco47I GGWCC 2 cut(s) 683, 830
EcoO109I RGGNCCY 2 cut(s) 830, 986
EcoRII CCWGG 2 cut(s) 625, 1054
EcoRV GATATC 1 cut(s) 808
EcoT14I CCWWGG 2 cut(s) 989, 1194
EcoT22I ATGCAT 1 cut(s) 926
ErhI CCWWGG 2 cut(s) 989, 1194
FaeI CATG 6 cut(s) 210, 374, 928, 1126, 1192, 1198
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FaqI GGGAC 2 cut(s) 893, 950
FatI CATG 6 cut(s) 206, 370, 924, 1122, 1188, 1194
Fnu4HI GCNGC 3 cut(s) 839, 872, 929
FokI GGATG 2 cut(s) 310, 503
Fsp4HI GCNGC 3 cut(s) 839, 872, 929
FspBI CTAG 2 cut(s) 359, 1179
GlaI GCGC 1 cut(s) 97
GluI GCNGC 3 cut(s) 839, 872, 929
GsaI CCCAGC 1 cut(s) 888
HaeIII GGCC 2 cut(s) 87, 988
HapII CCGG 9 cut(s) 93, 424, 465, 498, 520, 617, 789, 911, 934
HhaI GCGC 1 cut(s) 98
Hin1II CATG 6 cut(s) 210, 374, 928, 1126, 1192, 1198
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HincII GTYRAC 4 cut(s) 376, 418, 717, 949
HindII GTYRAC 4 cut(s) 376, 418, 717, 949
HinfI GANTC 4 cut(s) 7, 162, 459, 889
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 9 cut(s) 93, 424, 465, 498, 520, 617, 789, 911, 934
HphI GGTGA 4 cut(s) 82, 454, 641, 778
Hpy166II GTNNAC 6 cut(s) 145, 376, 418, 717, 949, 1004
Hpy188I TCNGA 4 cut(s) 220, 477, 567, 964
Hpy188III TCNNGA 5 cut(s) 157, 182, 655, 770, 1022
Hpy8I GTNNAC 6 cut(s) 145, 376, 418, 717, 949, 1004
HpyAV CCTTC 2 cut(s) 326, 414
HpyCH4III ACNGT 5 cut(s) 248, 283, 400, 683, 1115
HpyCH4IV ACGT 4 cut(s) 195, 506, 981, 1026
HpyCH4V TGCA 5 cut(s) 137, 228, 924, 1041, 1072
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 359, 985
HpyF3I CTNAG 2 cut(s) 121, 1101
HpySE526I ACGT 4 cut(s) 195, 506, 981, 1026
Hsp92II CATG 6 cut(s) 210, 374, 928, 1126, 1192, 1198
HspAI GCGC 1 cut(s) 96
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 6 cut(s) 153, 343, 707, 774, 801, 1064
LmnI GCTCC 4 cut(s) 325, 791, 936, 1207
Lsp1109I GCAGC 2 cut(s) 883, 915
LweI GCATC 3 cut(s) 708, 803, 1059
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 2 cut(s) 359, 1179
MaeII ACGT 4 cut(s) 195, 506, 981, 1026
MaeIII GTNAC 5 cut(s) 168, 196, 248, 277, 1031
MalI GATC 6 cut(s) 155, 345, 709, 776, 803, 1066
MboI GATC 6 cut(s) 153, 343, 707, 774, 801, 1064
MboII GAAGA 7 cut(s) 574, 643, 727, 1042, 1158, 1161, 1167
MfeI CAATTG 2 cut(s) 71, 1036
MluCI AATT 4 cut(s) 71, 347, 385, 1036
MmeI TCCRAC 4 cut(s) 16, 469, 500, 942
Mph1103I ATGCAT 1 cut(s) 926
MseI TTAA 4 cut(s) 375, 704, 722, 1213
MslI CAYNNNNRTG 1 cut(s) 1193
MspA1I CMGCKG 1 cut(s) 482
MspCI CTTAAG 1 cut(s) 703
MspI CCGG 9 cut(s) 93, 424, 465, 498, 520, 617, 789, 911, 934
MspR9I CCNGG 5 cut(s) 617, 627, 911, 935, 1056
MunI CAATTG 2 cut(s) 71, 1036
Mva1269I GAATGC 1 cut(s) 1041
MvaI CCWGG 2 cut(s) 627, 1056
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 93, 359, 985
NciI CCSGG 3 cut(s) 617, 911, 935
NcoI CCATGG 1 cut(s) 1194
NdeII GATC 6 cut(s) 153, 343, 707, 774, 801, 1064
NlaIII CATG 6 cut(s) 210, 374, 928, 1126, 1192, 1198
NlaIV GGNNCC 7 cut(s) 40, 402, 782, 831, 904, 1130, 1203
NmuCI GTSAC 3 cut(s) 168, 196, 248
NsiI ATGCAT 1 cut(s) 926
NspI RCATGY 2 cut(s) 928, 1126
PaeI GCATGC 1 cut(s) 928
PciI ACATGT 1 cut(s) 1122
PctI GAATGC 1 cut(s) 1041
PfeI GAWTC 4 cut(s) 7, 162, 459, 889
PflMI CCANNNNNTGG 1 cut(s) 207
PfoI TCCNGGA 3 cut(s) 615, 909, 933
PinAI ACCGGT 1 cut(s) 464
PkrI GCNGC 3 cut(s) 840, 873, 930
Ppu21I YACGTR 1 cut(s) 982
PpuMI RGGWCCY 1 cut(s) 830
PscI ACATGT 1 cut(s) 1122
PsiI TTATAA 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 830
Psp6I CCWGG 2 cut(s) 625, 1054
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 2 cut(s) 625, 1054
PspN4I GGNNCC 7 cut(s) 40, 402, 782, 831, 904, 1130, 1203
PspPI GGNCC 3 cut(s) 683, 830, 986
PspPPI RGGWCCY 1 cut(s) 830
PstI CTGCAG 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 5 cut(s) 146, 397, 637, 732, 1109
RsaNI GTAC 5 cut(s) 145, 396, 636, 731, 1108
RseI CAYNNNNRTG 1 cut(s) 1193
SaqAI TTAA 4 cut(s) 375, 704, 722, 1213
SatI GCNGC 3 cut(s) 839, 872, 929
Sau3AI GATC 6 cut(s) 153, 343, 707, 774, 801, 1064
Sau96I GGNCC 3 cut(s) 683, 830, 986
ScaI AGTACT 2 cut(s) 732, 1109
ScrFI CCNGG 5 cut(s) 617, 627, 911, 935, 1056
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 3 cut(s) 708, 803, 1059
SfcI CTRYAG 1 cut(s) 226
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 2 cut(s) 683, 830
SmiMI CAYNNNNRTG 1 cut(s) 1193
SmlI CTYRAG 1 cut(s) 703
SmoI CTYRAG 1 cut(s) 703
SphI GCATGC 1 cut(s) 928
Sse9I AATT 4 cut(s) 71, 347, 385, 1036
SsiI CCGC 3 cut(s) 273, 839, 1082
SspMI CTAG 2 cut(s) 359, 1179
StyD4I CCNGG 5 cut(s) 615, 625, 909, 933, 1054
StyI CCWWGG 2 cut(s) 989, 1194
TaaI ACNGT 5 cut(s) 248, 283, 400, 683, 1115
TaiI ACGT 4 cut(s) 198, 509, 984, 1029
TaqI TCGA 8 cut(s) 183, 294, 330, 342, 441, 573, 804, 1067
TaqII GACCGA 1 cut(s) 551
TasI AATT 4 cut(s) 71, 347, 385, 1036
TatI WGTACW 2 cut(s) 730, 1107
TauI GCSGC 1 cut(s) 841
TfiI GAWTC 4 cut(s) 7, 162, 459, 889
Tru1I TTAA 4 cut(s) 375, 704, 722, 1213
Tru9I TTAA 4 cut(s) 375, 704, 722, 1213
TscAI CASTG 5 cut(s) 144, 156, 251, 610, 804
TseFI GTSAC 3 cut(s) 168, 196, 248
TseI GCWGC 2 cut(s) 871, 928
Tsp45I GTSAC 3 cut(s) 168, 196, 248
TspDTI ATGAA 5 cut(s) 20, 359, 450, 574, 1177
TspRI CASTG 5 cut(s) 144, 156, 251, 610, 804
Van91I CCANNNNNTGG 1 cut(s) 207
Vha464I CTTAAG 1 cut(s) 703
VpaK11BI GGWCC 2 cut(s) 683, 830
XceI RCATGY 2 cut(s) 928, 1126
XspI CTAG 2 cut(s) 359, 1179
ZrmI AGTACT 2 cut(s) 732, 1109
Zsp2I ATGCAT 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.