pycom08g07700

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014329
Physical Location & Seq
Forward (+)
6201118 .. 6201774
657 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07700.1

Sequence Viewer

Length: 657 bp
ATGCTAAGGTTCAATTTCTTCACTGGGACCGTCCCGCCACAAATTTTCACTCAAGAACTCGACGCCCTCTTTTTAAACAACAACGATTTCATGCAAAATCTTCCCGACAATCTCGGCACCTCTCAAATTCTCCTCCTCACCTTAGCAAACAACAAATTCACAGGTTCGATTCCATCGGGCATTTCCAAAGCTTTCGCTGCTCTGACTGAGGTTCTACCCCTAAACAACCAGCTCACAGGATGCTTGCCTTATGAGATAGGGTTGCTAAAAGAGGCCGTATTTTTTGACGTTGGAAACAATCAGTTAACCGGGCCATTACCGTTCTCATTGGCCTGCCTCGAAAACGTGGAGCAGCTGAATTTTGCTGGGAATTTGTTGCATGGCATGGTGCCTGAGGTGGTATGTCATGAGCTGCAGAATTTGGCAAACTTTTCGCTGTCCGATAATTATTTCACAAATGTTGGTCCTGTTTGTAGAAGCTTGATCGGGAAAGGAGTGCTCGACGTTACAAACAACTGCGTTCCCGATCTTCCGTTTCAAAGACCGACAGAGGAATGTGCAGATTTCTTTGCACACCCAAGGTTCTGTCCCTTCATGGGGTCCTATACTTACATTCCATGTAAGCTTCCTCGTTTTGGTTCTTTGTATTCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

24.08

Weight (kDa)

4.7

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 116, 388
AciI CCGC 1 cut(s) 35
AcsI RAATTY 6 cut(s) 42, 126, 155, 358, 370, 418
AcyI GRCGYC 1 cut(s) 63
AfiI CCNNNNNNNGG 3 cut(s) 596, 597, 635
AgsI TTSAA 2 cut(s) 13, 539
AluBI AGCT 6 cut(s) 191, 232, 355, 412, 480, 625
AluI AGCT 6 cut(s) 191, 232, 355, 412, 480, 625
Alw21I GWGCWC 1 cut(s) 501
AoxI GGCC 3 cut(s) 273, 311, 330
ApeKI GCWGC 3 cut(s) 197, 352, 412
ApoI RAATTY 6 cut(s) 42, 126, 155, 358, 370, 418
AspS9I GGNCC 4 cut(s) 27, 311, 464, 600
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 2 cut(s) 130, 642
AvaII GGWCC 3 cut(s) 27, 464, 600
AxyI CCTNAGG 1 cut(s) 393
BanI GGYRCC 2 cut(s) 116, 388
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 3 cut(s) 184, 364, 399
BccI CCATC 1 cut(s) 181
BceAI ACGGC 1 cut(s) 260
BcgI CGANNNNNNTGC 2 cut(s) 414, 448
BcnI CCSGG 1 cut(s) 310
BfmI CTRYAG 1 cut(s) 413
BisI GCNGC 3 cut(s) 198, 353, 413
BlsI GCNGC 3 cut(s) 199, 354, 414
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 3 cut(s) 27, 464, 600
BmgT120I GGNCC 4 cut(s) 27, 311, 464, 600
BmiI GGNNCC 4 cut(s) 28, 118, 390, 601
BmrFI CCNGG 1 cut(s) 310
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 1 cut(s) 230
BmuI ACTGGG 1 cut(s) 33
Bpu10I CCTNAGC 2 cut(s) 5, 142
BpuEI CTTGAG 1 cut(s) 36
BpuMI CCSGG 1 cut(s) 310
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 1 cut(s) 578
Bsc4I CCNNNNNNNGG 3 cut(s) 596, 597, 635
Bse1I ACTGG 1 cut(s) 28
Bse21I CCTNAGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 1 cut(s) 245
BseLI CCNNNNNNNGG 3 cut(s) 596, 597, 635
BseMII CTCAG 2 cut(s) 198, 384
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 2 cut(s) 122, 125
BseXI GCAGC 3 cut(s) 184, 364, 399
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 579
BshFI GGCC 3 cut(s) 275, 313, 332
BshNI GGYRCC 2 cut(s) 116, 388
BsiHKAI GWGCWC 1 cut(s) 501
BsiSI CCGG 1 cut(s) 309
BslFI GGGAC 3 cut(s) 17, 40, 573
BslI CCNNNNNNNGG 3 cut(s) 596, 597, 635
BsmFI GGGAC 3 cut(s) 17, 40, 573
BsnI GGCC 3 cut(s) 275, 313, 332
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 2 cut(s) 483, 526
BspACI CCGC 1 cut(s) 35
BspANI GGCC 3 cut(s) 275, 313, 332
BspCNI CTCAG 2 cut(s) 199, 385
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 4 cut(s) 28, 118, 390, 601
BspMAI CTGCAG 1 cut(s) 417
BspT107I GGYRCC 2 cut(s) 116, 388
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 2 cut(s) 483, 526
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 31, 321
BstACI GRCGYC 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 245, 334
BstDEI CTNAG 4 cut(s) 5, 142, 207, 393
BstF5I GGATG 1 cut(s) 245
BstKTI GATC 2 cut(s) 486, 529
BstMBI GATC 2 cut(s) 483, 526
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 308
BstSFI CTRYAG 1 cut(s) 413
BstV1I GCAGC 3 cut(s) 184, 364, 399
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 3 cut(s) 275, 313, 332
BtsCI GGATG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 2 cut(s) 245, 334
CciI TCATGA 1 cut(s) 406
Cfr13I GGNCC 4 cut(s) 27, 311, 464, 600
CseI GACGC 1 cut(s) 71
CviAII CATG 6 cut(s) 91, 380, 385, 407, 595, 618
CviJI RGCY 9 cut(s) 191, 232, 275, 313, 332, 355, 412, 480, 625
CviKI_1 RGCY 9 cut(s) 191, 232, 275, 313, 332, 355, 412, 480, 625
DdeI CTNAG 4 cut(s) 5, 142, 207, 393
DpnI GATC 2 cut(s) 485, 528
DpnII GATC 2 cut(s) 483, 526
DraI TTTAAA 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 578
Eco47I GGWCC 3 cut(s) 27, 464, 600
Eco81I CCTNAGG 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 578
ErhI CCWWGG 1 cut(s) 578
FaeI CATG 6 cut(s) 94, 383, 388, 410, 598, 621
FaiI YATR 9 cut(s) 92, 252, 381, 386, 403, 408, 596, 606, 619
FaqI GGGAC 3 cut(s) 17, 40, 573
FatI CATG 6 cut(s) 90, 379, 384, 406, 594, 617
FauI CCCGC 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 198, 353, 413
FokI GGATG 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 198, 353, 413
GluI GCNGC 3 cut(s) 198, 353, 413
GsaI CCCAGC 1 cut(s) 369
HaeIII GGCC 3 cut(s) 275, 313, 332
HapII CCGG 1 cut(s) 309
HgaI GACGC 1 cut(s) 71
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 6 cut(s) 94, 383, 388, 410, 598, 621
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HindIII AAGCTT 3 cut(s) 189, 478, 623
HinfI GANTC 1 cut(s) 169
HpaI GTTAAC 1 cut(s) 306
HpaII CCGG 1 cut(s) 309
HphI GGTGA 2 cut(s) 130, 642
Hpy166II GTNNAC 1 cut(s) 306
Hpy188I TCNGA 2 cut(s) 204, 442
Hpy188III TCNNGA 5 cut(s) 53, 104, 407, 487, 524
Hpy8I GTNNAC 1 cut(s) 306
Hpy99I CGWCG 2 cut(s) 65, 506
HpyAV CCTTC 1 cut(s) 601
HpyCH4III ACNGT 2 cut(s) 31, 321
HpyCH4IV ACGT 3 cut(s) 288, 345, 504
HpyCH4V TGCA 5 cut(s) 94, 379, 415, 560, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 4 cut(s) 5, 142, 207, 393
HpySE526I ACGT 3 cut(s) 288, 345, 504
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 6 cut(s) 94, 383, 388, 410, 598, 621
KspAI GTTAAC 1 cut(s) 306
Kzo9I GATC 2 cut(s) 483, 526
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 9 cut(s) 9, 147, 222, 242, 322, 346, 351, 405, 480
Lsp1109I GCAGC 3 cut(s) 184, 364, 399
LweI GCATC 1 cut(s) 230
MaeII ACGT 3 cut(s) 288, 345, 504
MaeIII GTNAC 1 cut(s) 505
MalI GATC 2 cut(s) 485, 528
MboI GATC 2 cut(s) 483, 526
MboII GAAGA 3 cut(s) 10, 92, 521
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 8 cut(s) 13, 42, 126, 155, 358, 370, 418, 445
MmeI TCCRAC 1 cut(s) 271
MseI TTAA 2 cut(s) 74, 305
MspA1I CMGCKG 1 cut(s) 355
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 1 cut(s) 310
MwoI GCNNNNNNNGC 1 cut(s) 197
NciI CCSGG 1 cut(s) 310
NdeII GATC 2 cut(s) 483, 526
NlaIII CATG 6 cut(s) 94, 383, 388, 410, 598, 621
NlaIV GGNNCC 4 cut(s) 28, 118, 390, 601
NmeAIII GCCGAG 1 cut(s) 93
PagI TCATGA 1 cut(s) 406
PfeI GAWTC 1 cut(s) 169
PkrI GCNGC 3 cut(s) 199, 354, 414
PpuMI RGGWCCY 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 365
PspN4I GGNNCC 4 cut(s) 28, 118, 390, 601
PspPI GGNCC 4 cut(s) 27, 311, 464, 600
PspPPI RGGWCCY 1 cut(s) 600
PstI CTGCAG 1 cut(s) 417
PvuII CAGCTG 1 cut(s) 355
SaqAI TTAA 2 cut(s) 74, 305
SatI GCNGC 3 cut(s) 198, 353, 413
Sau3AI GATC 2 cut(s) 483, 526
Sau96I GGNCC 4 cut(s) 27, 311, 464, 600
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 1 cut(s) 230
SfcI CTRYAG 1 cut(s) 413
SinI GGWCC 3 cut(s) 27, 464, 600
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
Sse9I AATT 8 cut(s) 13, 42, 126, 155, 358, 370, 418, 445
SsiI CCGC 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 308
StyI CCWWGG 1 cut(s) 578
TaaI ACNGT 2 cut(s) 31, 321
TaiI ACGT 3 cut(s) 291, 348, 507
TaqI TCGA 4 cut(s) 60, 167, 339, 501
TaqII GACCGA 1 cut(s) 559
TasI AATT 8 cut(s) 13, 42, 126, 155, 358, 370, 418, 445
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 2 cut(s) 74, 305
Tru9I TTAA 2 cut(s) 74, 305
TscAI CASTG 1 cut(s) 28
TseI GCWGC 3 cut(s) 197, 352, 412
TspDTI ATGAA 2 cut(s) 79, 583
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 3 cut(s) 27, 464, 600
XapI RAATTY 6 cut(s) 42, 126, 155, 358, 370, 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.