pycom15g07290

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014329
Physical Location & Seq
Reverse (-)
4458110 .. 4459510
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g07290.1

Sequence Viewer

Length: 1401 bp
ATGAGAAGCCCTGATTCCACCAAAGCTCTAGTTTCATTCTCAACCTTCTTTTTTCTTCTAACCTATCTTTGTATTGCTAGTTGTGTAGCAGTAGCATTTCCGTTGCATGAAAATTCTTCTCCCGTAACAAACTATTCCAACAAACAAACGCTCGAGTTCGATTTAGGTGGCCACCGCAGAAGAGGCAGCGGACACAAAAGCCACCTAAAGCACAAAACCCCACCACCACCACCACCACCACCACCACCACCACCGGTACCACCACCACAAGTACCACTACCGCCGCCACAAGCACCTCCTCCTACAAAATTGCCAGACAACCAAGAACTTGTATTTGCAGACCAAAGGCTCAAAATAGTCTACCCAATTATCCAAAACTTCAAGTCCCTCATCACCTCAGATCCACTAAACATCACAAAAACTTGGGTGGGATCCAAAATATGCAGCTACAAAGGCTTCTACTGTGACAGCCCTCCAAACAACAGCTCCGCCATTGCCCTCGCCTCGATCGACTTCAACGGATTCCAACTAGGGGCCCCTTCTCTCGACGGATTTCTCGACCAGCTCCCGGACATTGCCCTCTTCCATGCAAACTCCAACAATTTTTCCGGCACTGTCTCCCCCGCCATTGCCAACCTCCCTTACCTCTACGAACTCGACATAAGCAACAACCAATTCTCCGGGGCGTTTCCCTCCGCCGTTCTCGGCATGAACACCTTAGGTTTCTTGGACATAAGGTTCAATTTCTTCACTGGGGCCGTCCCACCGCAGATTTTCACTCAAGAACTTGACGCCCTATTCCTAAACAACAACAATTTCATGCAGAATCTTCCCGACAATCTCGGCAGTTCTCATATTCTCCTTCTCACCTTAGCAAACAACAAATTCACGGGCTCAATTCCATCGGGCATTTCAAAAGCTTTCGCCGCTCTGACTGAGGTTCTGCTCCTAAACAACCAGCTCACAGGATGCTTGCCTTACGAGATAGGGTTGCTAAAACAGGCCGTAGTTTTAGATTTCGGAAACAACCAGTTAACCGGGCCGTTACCGTTTTCACTGGCCTGTCTCGAAAACGTGGAGCAGCTGAATTTTGCGGGTAACTTGTTGCATGGCATGGTACCTGAGGTGTTGTGTCATGAGCTGCAAAGCTTGGCGAACTTTTCGCTGTCCCATAATTACTTCACTAATGTTGGTCCGATTTGTAGAAGCTTGATCGGGAAAGGAGTGCTCGATGTTACGAACAACTGTGTTCCCGATCTTCTGTTTCAGAGACCGGTGGAGGAATGTGCGGATTTCTTTGCGCACCCAAGGTTTTGTCCCTTCATGTGGACTTATACTTACATTCCATGTAAGCTTCCTGGTTTTGGTTCTTTGTTTCCTCCATTGCCTCCTCCACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

467

Amino Acids

50.93

Weight (kDa)

5.98

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1302
Acc65I GGTACC 2 cut(s) 256, 1117
AccB1I GGYRCC 2 cut(s) 256, 1117
AccBSI CCGCTC 1 cut(s) 929
AccI GTMKAC 1 cut(s) 360
AclWI GGATC 3 cut(s) 395, 426, 439
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 3 cut(s) 112, 884, 1087
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 3 cut(s) 258, 273, 1119
AfiI CCNNNNNNNGG 4 cut(s) 532, 568, 1326, 1364
AgeI ACCGGT 2 cut(s) 253, 1273
AgsI TTSAA 4 cut(s) 382, 517, 742, 915
AjnI CCWGG 1 cut(s) 1357
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
Alw21I GWGCWC 1 cut(s) 1230
Alw26I GTCTC 3 cut(s) 622, 1070, 1264
AlwI GGATC 3 cut(s) 395, 426, 439
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 6 cut(s) 169, 534, 756, 1002, 1040, 1059
ApaI GGGCCC 1 cut(s) 538
ApeKI GCWGC 4 cut(s) 186, 444, 1081, 1141
ApoI RAATTY 3 cut(s) 112, 884, 1087
AsiGI ACCGGT 2 cut(s) 253, 1273
Asp718I GGTACC 2 cut(s) 256, 1117
AspLEI GCGC 1 cut(s) 1303
AspS9I GGNCC 5 cut(s) 534, 535, 756, 1040, 1193
AsuC2I CCSGG 3 cut(s) 569, 682, 1039
AsuHPI GGTGA 2 cut(s) 385, 859
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 1193
AxyI CCTNAGG 2 cut(s) 718, 1122
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 1 cut(s) 171
BamHI GGATCC 1 cut(s) 431
BanI GGYRCC 2 cut(s) 256, 1117
BanII GRGCYC 2 cut(s) 538, 896
Bbv12I GWGCWC 1 cut(s) 1230
BbvI GCAGC 4 cut(s) 198, 456, 1093, 1128
BccI CCATC 1 cut(s) 910
BceAI ACGGC 4 cut(s) 683, 743, 989, 1027
BciT130I CCWGG 1 cut(s) 1359
BcnI CCSGG 3 cut(s) 569, 682, 1039
BcoDI GTCTC 3 cut(s) 622, 1070, 1264
BfaI CTAG 3 cut(s) 29, 78, 530
BisI GCNGC 6 cut(s) 187, 284, 445, 927, 1082, 1142
BlsI GCNGC 6 cut(s) 188, 285, 446, 928, 1083, 1143
Bme1390I CCNGG 4 cut(s) 569, 682, 1039, 1359
Bme18I GGWCC 1 cut(s) 1193
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 5 cut(s) 534, 535, 756, 1040, 1193
BmiI GGNNCC 7 cut(s) 258, 433, 535, 536, 537, 757, 1119
BmrFI CCNGG 4 cut(s) 569, 682, 1039, 1359
BmrI ACTGGG 1 cut(s) 762
BmsI GCATC 1 cut(s) 959
BmuI ACTGGG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 871
BpuEI CTTGAG 1 cut(s) 765
BpuMI CCSGG 3 cut(s) 569, 682, 1039
BsaHI GRCGYC 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 1264
BsaJI CCNNGG 2 cut(s) 681, 1307
BsaWI WCCGGW 2 cut(s) 253, 1273
BsaXI ACNNNNNCTCC 4 cut(s) 470, 500, 662, 692
Bsc4I CCNNNNNNNGG 4 cut(s) 532, 568, 1326, 1364
Bse118I RCCGGY 2 cut(s) 253, 1273
Bse1I ACTGG 3 cut(s) 757, 1030, 1062
Bse21I CCTNAGG 2 cut(s) 718, 1122
Bse3DI GCAATG 4 cut(s) 492, 573, 627, 1382
BseBI CCWGG 1 cut(s) 1359
BseDI CCNNGG 2 cut(s) 681, 1307
BseGI GGATG 1 cut(s) 974
BseLI CCNNNNNNNGG 4 cut(s) 532, 568, 1326, 1364
BseMI GCAATG 4 cut(s) 492, 573, 627, 1382
BseMII CTCAG 3 cut(s) 411, 927, 1113
BseNI ACTGG 3 cut(s) 757, 1030, 1062
BseRI GAGGAG 2 cut(s) 288, 1380
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 4 cut(s) 198, 456, 1093, 1128
Bsh1285I CGRYCG 1 cut(s) 510
BshFI GGCC 6 cut(s) 171, 536, 758, 1004, 1042, 1061
BshNI GGYRCC 2 cut(s) 256, 1117
BshTI ACCGGT 2 cut(s) 253, 1273
BsiEI CGRYCG 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 1230
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 6 cut(s) 254, 569, 609, 681, 1038, 1274
BslFI GGGAC 4 cut(s) 370, 746, 1153, 1302
BslI CCNNNNNNNGG 4 cut(s) 532, 568, 1326, 1364
BsmAI GTCTC 3 cut(s) 622, 1070, 1264
BsmFI GGGAC 4 cut(s) 370, 746, 1153, 1302
BsnI GGCC 6 cut(s) 171, 536, 758, 1004, 1042, 1061
Bso31I GGTCTC 1 cut(s) 1264
BsoBI CYCGRG 1 cut(s) 152
Bsp120I GGGCCC 1 cut(s) 534
Bsp1286I GDGCHC 3 cut(s) 538, 896, 1230
Bsp143I GATC 5 cut(s) 400, 431, 507, 1212, 1255
BspANI GGCC 6 cut(s) 171, 536, 758, 1004, 1042, 1061
BspCNI CTCAG 3 cut(s) 410, 928, 1114
BspHI TCATGA 1 cut(s) 1135
BspLI GGNNCC 7 cut(s) 258, 433, 535, 536, 537, 757, 1119
BspPI GGATC 3 cut(s) 395, 426, 439
BspT107I GGYRCC 2 cut(s) 256, 1117
BspTNI GGTCTC 1 cut(s) 1264
BsrBI CCGCTC 1 cut(s) 929
BsrDI GCAATG 4 cut(s) 492, 573, 627, 1382
BsrFI RCCGGY 2 cut(s) 253, 1273
BsrI ACTGG 3 cut(s) 757, 1030, 1062
BssAI RCCGGY 2 cut(s) 253, 1273
BssECI CCNNGG 2 cut(s) 681, 1307
BssMI GATC 5 cut(s) 400, 431, 507, 1212, 1255
BssNI GRCGYC 1 cut(s) 792
BssT1I CCWWGG 1 cut(s) 1307
Bst2UI CCWGG 1 cut(s) 1359
Bst4CI ACNGT 5 cut(s) 464, 616, 1050, 1247, 1398
Bst6I CTCTTC 2 cut(s) 175, 587
BstACI GRCGYC 1 cut(s) 792
BstC8I GCNNGC 1 cut(s) 974
BstDEI CTNAG 5 cut(s) 397, 718, 871, 936, 1122
BstF5I GGATG 1 cut(s) 974
BstHHI GCGC 1 cut(s) 1303
BstKTI GATC 5 cut(s) 403, 434, 510, 1215, 1258
BstMAI GTCTC 3 cut(s) 622, 1070, 1264
BstMBI GATC 5 cut(s) 400, 431, 507, 1212, 1255
BstMCI CGRYCG 1 cut(s) 510
BstMWI GCNNNNNNNGC 3 cut(s) 183, 453, 926
BstNI CCWGG 1 cut(s) 1359
BstSCI CCNGG 4 cut(s) 567, 680, 1037, 1357
BstSLI GKGCMC 1 cut(s) 538
BstV1I GCAGC 4 cut(s) 198, 456, 1093, 1128
BstX2I RGATCY 2 cut(s) 400, 431
BstYI RGATCY 2 cut(s) 400, 431
Bsu36I CCTNAGG 2 cut(s) 718, 1122
BsuRI GGCC 6 cut(s) 171, 536, 758, 1004, 1042, 1061
BtsCI GGATG 1 cut(s) 974
BtsIMutI CAGTG 3 cut(s) 612, 750, 1055
Cac8I GCNNGC 1 cut(s) 974
CciI TCATGA 1 cut(s) 1135
CfoI GCGC 1 cut(s) 1303
Cfr10I RCCGGY 2 cut(s) 253, 1273
Cfr13I GGNCC 5 cut(s) 534, 535, 756, 1040, 1193
CseI GACGC 1 cut(s) 800
Csp6I GTAC 3 cut(s) 257, 272, 1118
CspAI ACCGGT 2 cut(s) 253, 1273
CviAII CATG 9 cut(s) 107, 587, 709, 820, 1109, 1114, 1136, 1324, 1347
CviQI GTAC 3 cut(s) 257, 272, 1118
DdeI CTNAG 5 cut(s) 397, 718, 871, 936, 1122
DpnI GATC 5 cut(s) 402, 433, 509, 1214, 1257
DpnII GATC 5 cut(s) 400, 431, 507, 1212, 1255
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 2 cut(s) 175, 587
EarI CTCTTC 2 cut(s) 175, 587
EciI GGCGGA 2 cut(s) 478, 685
Eco130I CCWWGG 1 cut(s) 1307
Eco24I GRGCYC 2 cut(s) 538, 896
Eco31I GGTCTC 1 cut(s) 1264
Eco47I GGWCC 1 cut(s) 1193
Eco81I CCTNAGG 2 cut(s) 718, 1122
Eco88I CYCGRG 1 cut(s) 152
EcoO109I RGGNCCY 2 cut(s) 534, 535
EcoRII CCWGG 1 cut(s) 1357
EcoT14I CCWWGG 1 cut(s) 1307
EcoT38I GRGCYC 2 cut(s) 538, 896
ErhI CCWWGG 1 cut(s) 1307
FaeI CATG 9 cut(s) 110, 590, 712, 823, 1112, 1117, 1139, 1327, 1350
FaqI GGGAC 4 cut(s) 370, 746, 1153, 1302
FatI CATG 9 cut(s) 106, 586, 708, 819, 1108, 1113, 1135, 1323, 1346
FauI CCCGC 2 cut(s) 631, 1087
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 6 cut(s) 187, 284, 445, 927, 1082, 1142
FokI GGATG 1 cut(s) 981
FriOI GRGCYC 2 cut(s) 538, 896
Fsp4HI GCNGC 6 cut(s) 187, 284, 445, 927, 1082, 1142
FspBI CTAG 3 cut(s) 29, 78, 530
FspI TGCGCA 1 cut(s) 1302
GlaI GCGC 1 cut(s) 1302
GluI GCNGC 6 cut(s) 187, 284, 445, 927, 1082, 1142
HaeIII GGCC 6 cut(s) 171, 536, 758, 1004, 1042, 1061
HapII CCGG 6 cut(s) 254, 569, 609, 681, 1038, 1274
HgaI GACGC 1 cut(s) 800
HhaI GCGC 1 cut(s) 1303
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 9 cut(s) 110, 590, 712, 823, 1112, 1117, 1139, 1327, 1350
Hin6I GCGC 1 cut(s) 1301
HinP1I GCGC 1 cut(s) 1301
HincII GTYRAC 1 cut(s) 1035
HindII GTYRAC 1 cut(s) 1035
HindIII AAGCTT 4 cut(s) 918, 1147, 1207, 1352
HinfI GANTC 3 cut(s) 14, 522, 826
HpaI GTTAAC 1 cut(s) 1035
HpaII CCGG 6 cut(s) 254, 569, 609, 681, 1038, 1274
HphI GGTGA 2 cut(s) 385, 859
Hpy166II GTNNAC 3 cut(s) 361, 1035, 1329
Hpy188I TCNGA 5 cut(s) 400, 933, 1022, 1197, 1269
Hpy188III TCNNGA 8 cut(s) 545, 557, 782, 833, 1067, 1136, 1216, 1253
Hpy8I GTNNAC 3 cut(s) 361, 1035, 1329
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 4 cut(s) 55, 549, 872, 1330
HpyCH4III ACNGT 5 cut(s) 464, 616, 1050, 1247, 1398
HpyCH4IV ACGT 1 cut(s) 1074
HpyCH4V TGCA 7 cut(s) 106, 338, 444, 590, 823, 1108, 1144
HpyF10VI GCNNNNNNNGC 3 cut(s) 183, 453, 926
HpyF3I CTNAG 5 cut(s) 397, 718, 871, 936, 1122
HpySE526I ACGT 1 cut(s) 1074
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 9 cut(s) 110, 590, 712, 823, 1112, 1117, 1139, 1327, 1350
HspAI GCGC 1 cut(s) 1301
KpnI GGTACC 2 cut(s) 260, 1121
KspAI GTTAAC 1 cut(s) 1035
Kzo9I GATC 5 cut(s) 400, 431, 507, 1212, 1255
LmnI GCTCC 4 cut(s) 491, 570, 951, 1078
Lsp1109I GCAGC 4 cut(s) 198, 456, 1093, 1128
LweI GCATC 1 cut(s) 959
MaeI CTAG 3 cut(s) 29, 78, 530
MaeII ACGT 1 cut(s) 1074
MaeIII GTNAC 5 cut(s) 124, 464, 1044, 1097, 1234
MalI GATC 5 cut(s) 402, 433, 509, 1214, 1257
MbiI CCGCTC 1 cut(s) 929
MboI GATC 5 cut(s) 400, 431, 507, 1212, 1255
MboII GAAGA 7 cut(s) 47, 108, 192, 574, 739, 821, 1250
MflI RGATCY 2 cut(s) 400, 431
MhlI GDGCHC 3 cut(s) 538, 896, 1230
MlsI TGGCCA 1 cut(s) 171
MluNI TGGCCA 1 cut(s) 171
MmeI TCCRAC 3 cut(s) 162, 550, 621
Mox20I TGGCCA 1 cut(s) 171
MscI TGGCCA 1 cut(s) 171
MseI TTAA 1 cut(s) 1034
Msp20I TGGCCA 1 cut(s) 171
MspA1I CMGCKG 2 cut(s) 189, 1084
MspI CCGG 6 cut(s) 254, 569, 609, 681, 1038, 1274
MspR9I CCNGG 4 cut(s) 569, 682, 1039, 1359
MvaI CCWGG 1 cut(s) 1359
MwoI GCNNNNNNNGC 3 cut(s) 183, 453, 926
NciI CCSGG 3 cut(s) 569, 682, 1039
NdeII GATC 5 cut(s) 400, 431, 507, 1212, 1255
NlaIII CATG 9 cut(s) 110, 590, 712, 823, 1112, 1117, 1139, 1327, 1350
NlaIV GGNNCC 7 cut(s) 258, 433, 535, 536, 537, 757, 1119
NmeAIII GCCGAG 2 cut(s) 684, 822
NmuCI GTSAC 1 cut(s) 464
NsbI TGCGCA 1 cut(s) 1302
PaeR7I CTCGAG 1 cut(s) 152
PagI TCATGA 1 cut(s) 1135
PcsI WCGNNNNNNNCGW 2 cut(s) 507, 555
PfeI GAWTC 3 cut(s) 14, 522, 826
PfoI TCCNGGA 1 cut(s) 567
PinAI ACCGGT 2 cut(s) 253, 1273
PkrI GCNGC 6 cut(s) 188, 285, 446, 928, 1083, 1143
Ple19I CGATCG 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 1357
PspGI CCWGG 1 cut(s) 1357
PspN4I GGNNCC 7 cut(s) 258, 433, 535, 536, 537, 757, 1119
PspOMI GGGCCC 1 cut(s) 534
PspPI GGNCC 5 cut(s) 534, 535, 756, 1040, 1193
PspXI VCTCGAGB 1 cut(s) 152
PsuI RGATCY 2 cut(s) 400, 431
PvuI CGATCG 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 1084
RsaI GTAC 3 cut(s) 258, 273, 1119
RsaNI GTAC 3 cut(s) 257, 272, 1118
SaqAI TTAA 1 cut(s) 1034
SatI GCNGC 6 cut(s) 187, 284, 445, 927, 1082, 1142
Sau3AI GATC 5 cut(s) 400, 431, 507, 1212, 1255
Sau96I GGNCC 5 cut(s) 534, 535, 756, 1040, 1193
ScrFI CCNGG 4 cut(s) 569, 682, 1039, 1359
SduI GDGCHC 3 cut(s) 538, 896, 1230
SfaNI GCATC 1 cut(s) 959
Sfr274I CTCGAG 1 cut(s) 152
SinI GGWCC 1 cut(s) 1193
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 152, 780
SmoI CTYRAG 2 cut(s) 152, 780
SspMI CTAG 3 cut(s) 29, 78, 530
StyD4I CCNGG 4 cut(s) 567, 680, 1037, 1357
StyI CCWWGG 1 cut(s) 1307
TaaI ACNGT 5 cut(s) 464, 616, 1050, 1247, 1398
TaiI ACGT 1 cut(s) 1077
TaqI TCGA 9 cut(s) 153, 159, 506, 510, 546, 558, 657, 1068, 1230
TauI GCSGC 2 cut(s) 286, 929
TfiI GAWTC 3 cut(s) 14, 522, 826
Tru1I TTAA 1 cut(s) 1034
Tru9I TTAA 1 cut(s) 1034
TscAI CASTG 3 cut(s) 619, 757, 1062
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 4 cut(s) 186, 444, 1081, 1141
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 5 cut(s) 24, 123, 725, 808, 1312
TspGWI ACGGA 3 cut(s) 90, 534, 564
TspRI CASTG 3 cut(s) 619, 757, 1062
VpaK11BI GGWCC 1 cut(s) 1193
XapI RAATTY 3 cut(s) 112, 884, 1087
XhoI CTCGAG 1 cut(s) 152
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 3 cut(s) 29, 78, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.