RLG00000010639

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014329
Physical Location & Seq
Forward (+)
3196358 .. 3198769
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010639

Sequence Viewer

Length: 1278 bp
ATGAGAAGAAGCGCTTCAACCAAAGCTTTGTTTTTAACCTTCTTCTTCTTCCTCCTAACATGCCTCATTGCAAACTCCGCAACTCACAACATTACTAGAAGAAGTAGCAAAGACAAAACCCACAGAAAGAAGTGTCCTATAACCAGTAGTCTAGTCCAGTGTTCGAAAACACCGCCACCAGATTTACCAAACCCACAAAAAGTGCTCTCATTTGCAGACCAAAGGCTTCAAGTGGTATACCCAGTCATTCAAAAATTCAAGTCCATCATCACCTCAGATCCTCTCAACATCACCAAAACTTGGGTAGGATCAGATATATGCAAATACAAAGGCTTCTACTGTGACAATCCACCATACAACTTATCCGCAATCGCGCTAGCCTCCATCGACTTCAATGGCTTCCAACTCGGTGCTTCCTCCCTTGATGGTTTTCTTGATCAGCTCCCTGATATTGCTCTGTTCCATGCAAACTCCAACAACTTTTCCGGCACCATTTCCTCCAAAATTGCCAACCTCCCCTACCTCTATGAGCTTGACATAAGCAACAACTTATTCTCCGGTCCATTCCCAACCGCAGTTCTCGGCATGAACGGCTTAACTTTCTTGGACATAAGGTTCAATTTCTTCACCGGCTCTGTTCCGCCTCAGATCTTCACTCAGGACCTTGATGCTCTCTTCCTCAACAACAACAACTTCATGCAGAGCCTTCCTGATAACCTAGGCAGCTCCCATATAATCCTCCTCACTTTGGCAAACAACAACTTCAATGGCCCTATTCCAAGCAGCATTGCAAAAGCTCTGTCTTCTCTCACTGAAGTTCTGCTTCTCAATAACCAGTTGACAGGTTGTTTGCCTTACGAGCTAGGTTTTTTAAAGGAGGCCATAGTGTTTGATGCTAGCAACAATCAGTTAACTGGTCCGTTACCGTTCTCTTTGGCCTGTCTAGACAAAGTGGAGCAGCTAAGTTTTGCTGGAAACTTGTTGTTTGGCTTGGTGCCGGAGGTGATCTGTGATCAGCTGCTGAATTTGGCAAACTTTTCGCTGTCCAATAATTACTTCATCCATGTTGGTCCAATATGTCAAAGTTTGATCCAGAGAGGACTGCTTGATGTTACTAACAACTGCATTCCTGGTCTTCCATTCCAAAGATCGGTAGAAGAATGTGCAGATTTCTTCGCGCATCCCATGTTCTGTCCGAATATGGGGTCTTATACCTGTATACCCTGCAGTAAGTTTCCTTATGCTTCTTTTGTTCATCAAGTGGATCCTACACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

46.82

Weight (kDa)

6.15

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 488, 994
AccB7I CCANNNNNTGG 1 cut(s) 300
AccI GTMKAC 2 cut(s) 237, 1219
AccII CGCG 2 cut(s) 374, 1178
AciI CCGC 5 cut(s) 78, 173, 366, 573, 641
AclWI GGATC 5 cut(s) 272, 316, 1084, 1259, 1272
AcsI RAATTY 2 cut(s) 254, 1024
AcuI CTGAAG 1 cut(s) 834
AfeI AGCGCT 1 cut(s) 13
AfiI CCNNNNNNNGG 4 cut(s) 300, 748, 1150, 1202
AgsI TTSAA 7 cut(s) 18, 230, 251, 259, 394, 619, 766
AjnI CCWGG 1 cut(s) 1129
AjuI GAANNNNNNNTTGG 2 cut(s) 1040, 1072
AloI GAACNNNNNNTCC 2 cut(s) 599, 631
AluBI AGCT 8 cut(s) 26, 442, 532, 726, 797, 862, 961, 1018
AluI AGCT 8 cut(s) 26, 442, 532, 726, 797, 862, 961, 1018
Alw21I GWGCWC 1 cut(s) 207
AlwI GGATC 5 cut(s) 272, 316, 1084, 1259, 1272
AlwNI CAGNNNCTG 1 cut(s) 1021
Aor51HI AGCGCT 1 cut(s) 13
AoxI GGCC 3 cut(s) 769, 879, 936
ApeKI GCWGC 4 cut(s) 723, 783, 958, 1018
ApoI RAATTY 2 cut(s) 254, 1024
ArsI GACNNNNNNTTYG 6 cut(s) 785, 817, 832, 864, 1190, 1222
Asp700I GAANNNNTTC 1 cut(s) 13
AspA2I CCTAGG 1 cut(s) 718
AspLEI GCGC 3 cut(s) 14, 376, 1180
AspS9I GGNCC 5 cut(s) 560, 661, 770, 917, 1070
AsuHPI GGTGA 4 cut(s) 262, 283, 619, 1015
AsuII TTCGAA 1 cut(s) 164
AsuNHI GCTAGC 2 cut(s) 376, 896
AvaII GGWCC 4 cut(s) 560, 661, 917, 1070
AvrII CCTAGG 1 cut(s) 718
BamHI GGATCC 1 cut(s) 1264
BanI GGYRCC 2 cut(s) 488, 994
BbsI GAAGAC 2 cut(s) 795, 1127
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 4 cut(s) 735, 795, 970, 1005
BccI CCATC 3 cut(s) 272, 392, 419
BceAI ACGGC 1 cut(s) 607
BcgI CGANNNNNNTGC 2 cut(s) 1020, 1054
BciT130I CCWGG 1 cut(s) 1131
BclI TGATCA 2 cut(s) 436, 1012
BfaI CTAG 7 cut(s) 96, 152, 377, 719, 863, 897, 944
BfmI CTRYAG 1 cut(s) 1225
BfoI RGCGCY 1 cut(s) 15
BglII AGATCT 1 cut(s) 648
BisI GCNGC 4 cut(s) 724, 784, 959, 1019
BlnI CCTAGG 1 cut(s) 718
BlsI GCNGC 4 cut(s) 725, 785, 960, 1020
Bme1390I CCNGG 1 cut(s) 1131
Bme18I GGWCC 4 cut(s) 560, 661, 917, 1070
BmgT120I GGNCC 5 cut(s) 560, 661, 770, 917, 1070
BmiI GGNNCC 3 cut(s) 490, 996, 1266
BmrFI CCNGG 1 cut(s) 1131
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 3 cut(s) 658, 883, 1189
BmtI GCTAGC 2 cut(s) 380, 900
BmuI ACTGGG 1 cut(s) 236
BpiI GAAGAC 2 cut(s) 795, 1127
Bpu14I TTCGAA 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 718
BsaWI WCCGGW 1 cut(s) 557
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 4 cut(s) 300, 748, 1150, 1202
Bse118I RCCGGY 1 cut(s) 629
Bse1I ACTGG 5 cut(s) 144, 157, 242, 835, 919
Bse3DI GCAATG 2 cut(s) 66, 786
BseBI CCWGG 1 cut(s) 1131
BseDI CCNNGG 1 cut(s) 718
BseGI GGATG 2 cut(s) 1059, 1180
BseLI CCNNNNNNNGG 4 cut(s) 300, 748, 1150, 1202
BseMI GCAATG 2 cut(s) 66, 786
BseMII CTCAG 3 cut(s) 288, 659, 671
BseNI ACTGG 5 cut(s) 144, 157, 242, 835, 919
BseRI GAGGAG 1 cut(s) 731
BseXI GCAGC 4 cut(s) 735, 795, 970, 1005
BsgI GTGCAG 1 cut(s) 1185
Bsh1236I CGCG 2 cut(s) 374, 1178
BshFI GGCC 3 cut(s) 771, 881, 938
BshNI GGYRCC 2 cut(s) 488, 994
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 4 cut(s) 486, 558, 630, 998
BslI CCNNNNNNNGG 4 cut(s) 300, 748, 1150, 1202
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 3 cut(s) 771, 881, 938
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 9 cut(s) 277, 308, 436, 648, 1005, 1012, 1089, 1148, 1264
BspACI CCGC 5 cut(s) 78, 173, 366, 573, 641
BspANI GGCC 3 cut(s) 771, 881, 938
BspCNI CTCAG 3 cut(s) 287, 658, 670
BspFNI CGCG 2 cut(s) 374, 1178
BspLI GGNNCC 3 cut(s) 490, 996, 1266
BspMAI CTGCAG 1 cut(s) 1229
BspOI GCTAGC 2 cut(s) 380, 900
BspPI GGATC 5 cut(s) 272, 316, 1084, 1259, 1272
BspT104I TTCGAA 1 cut(s) 164
BspT107I GGYRCC 2 cut(s) 488, 994
BsrDI GCAATG 2 cut(s) 66, 786
BsrFI RCCGGY 1 cut(s) 629
BsrI ACTGG 5 cut(s) 144, 157, 242, 835, 919
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 1 cut(s) 718
BssMI GATC 9 cut(s) 277, 308, 436, 648, 1005, 1012, 1089, 1148, 1264
BssNAI GTATAC 2 cut(s) 238, 1220
BssT1I CCWWGG 1 cut(s) 718
Bst1107I GTATAC 2 cut(s) 238, 1220
Bst2UI CCWGG 1 cut(s) 1131
Bst4CI ACNGT 2 cut(s) 341, 927
Bst6I CTCTTC 1 cut(s) 680
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 378, 898
BstDEI CTNAG 4 cut(s) 274, 645, 657, 962
BstF5I GGATG 2 cut(s) 1059, 1180
BstFNI CGCG 2 cut(s) 374, 1178
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 3 cut(s) 14, 376, 1180
BstKTI GATC 9 cut(s) 280, 311, 439, 651, 1008, 1015, 1092, 1151, 1267
BstMBI GATC 9 cut(s) 277, 308, 436, 648, 1005, 1012, 1089, 1148, 1264
BstMWI GCNNNNNNNGC 3 cut(s) 77, 591, 859
BstNI CCWGG 1 cut(s) 1131
BstNSI RCATGY 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 1129
BstSFI CTRYAG 1 cut(s) 1225
BstUI CGCG 2 cut(s) 374, 1178
BstV1I GCAGC 4 cut(s) 735, 795, 970, 1005
BstV2I GAAGAC 2 cut(s) 795, 1127
BstX2I RGATCY 3 cut(s) 277, 648, 1264
BstYI RGATCY 3 cut(s) 277, 648, 1264
BstZ17I GTATAC 2 cut(s) 238, 1220
BsuRI GGCC 3 cut(s) 771, 881, 938
BtsCI GGATG 2 cut(s) 1059, 1180
BtsIMutI CAGTG 2 cut(s) 164, 810
Cac8I GCNNGC 2 cut(s) 378, 898
CaiI CAGNNNCTG 1 cut(s) 1021
CfoI GCGC 3 cut(s) 14, 376, 1180
Cfr10I RCCGGY 1 cut(s) 629
Cfr13I GGNCC 5 cut(s) 560, 661, 770, 917, 1070
CviAII CATG 6 cut(s) 60, 464, 586, 697, 1064, 1186
DdeI CTNAG 4 cut(s) 274, 645, 657, 962
DpnI GATC 9 cut(s) 279, 310, 438, 650, 1007, 1014, 1091, 1150, 1266
DpnII GATC 9 cut(s) 277, 308, 436, 648, 1005, 1012, 1089, 1148, 1264
DraI TTTAAA 1 cut(s) 873
Eam1104I CTCTTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 680
EciI GGCGGA 1 cut(s) 630
Eco130I CCWWGG 1 cut(s) 718
Eco47I GGWCC 4 cut(s) 560, 661, 917, 1070
Eco47III AGCGCT 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 834
EcoO109I RGGNCCY 1 cut(s) 661
EcoRII CCWGG 1 cut(s) 1129
EcoT14I CCWWGG 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 718
FaeI CATG 6 cut(s) 63, 467, 589, 700, 1067, 1189
FalI AAGNNNNNCTT 3 cut(s) 30, 807, 839
FatI CATG 6 cut(s) 59, 463, 585, 696, 1063, 1185
FbaI TGATCA 2 cut(s) 436, 1012
FblI GTMKAC 2 cut(s) 237, 1219
Fnu4HI GCNGC 4 cut(s) 724, 784, 959, 1019
FokI GGATG 2 cut(s) 1046, 1167
Fsp4HI GCNGC 4 cut(s) 724, 784, 959, 1019
FspBI CTAG 7 cut(s) 96, 152, 377, 719, 863, 897, 944
GlaI GCGC 3 cut(s) 13, 375, 1179
GluI GCNGC 4 cut(s) 724, 784, 959, 1019
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 3 cut(s) 771, 881, 938
HapII CCGG 4 cut(s) 486, 558, 630, 998
HhaI GCGC 3 cut(s) 14, 376, 1180
Hin1II CATG 6 cut(s) 63, 467, 589, 700, 1067, 1189
Hin6I GCGC 3 cut(s) 12, 374, 1178
HinP1I GCGC 3 cut(s) 12, 374, 1178
HincII GTYRAC 2 cut(s) 840, 912
HindII GTYRAC 2 cut(s) 840, 912
HindIII AAGCTT 1 cut(s) 24
HpaI GTTAAC 1 cut(s) 912
HpaII CCGG 4 cut(s) 486, 558, 630, 998
HphI GGTGA 4 cut(s) 262, 283, 619, 1015
Hpy166II GTNNAC 4 cut(s) 238, 840, 912, 1220
Hpy188I TCNGA 4 cut(s) 277, 313, 648, 1197
Hpy188III TCNNGA 5 cut(s) 434, 659, 710, 944, 1093
Hpy8I GTNNAC 4 cut(s) 238, 840, 912, 1220
HpyAV CCTTC 2 cut(s) 49, 716
HpyCH4III ACNGT 2 cut(s) 341, 927
HpyCH4V TGCA 9 cut(s) 71, 215, 321, 467, 700, 791, 1125, 1166, 1227
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 591, 859
HpyF3I CTNAG 4 cut(s) 274, 645, 657, 962
Hsp92II CATG 6 cut(s) 63, 467, 589, 700, 1067, 1189
HspAI GCGC 3 cut(s) 12, 374, 1178
Ksp22I TGATCA 2 cut(s) 436, 1012
KspAI GTTAAC 1 cut(s) 912
Kzo9I GATC 9 cut(s) 277, 308, 436, 648, 1005, 1012, 1089, 1148, 1264
LmnI GCTCC 3 cut(s) 447, 731, 955
Lsp1109I GCAGC 4 cut(s) 735, 795, 970, 1005
LweI GCATC 3 cut(s) 658, 883, 1189
MaeI CTAG 7 cut(s) 96, 152, 377, 719, 863, 897, 944
MaeIII GTNAC 3 cut(s) 341, 921, 1111
MalI GATC 9 cut(s) 279, 310, 438, 650, 1007, 1014, 1091, 1150, 1266
MboI GATC 9 cut(s) 277, 308, 436, 648, 1005, 1012, 1089, 1148, 1264
MflI RGATCY 3 cut(s) 277, 648, 1264
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 5 cut(s) 254, 504, 619, 1024, 1051
MmeI TCCRAC 2 cut(s) 427, 498
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 4 cut(s) 35, 596, 872, 911
MspA1I CMGCKG 1 cut(s) 1018
MspI CCGG 4 cut(s) 486, 558, 630, 998
MspR9I CCNGG 1 cut(s) 1131
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 1 cut(s) 1131
MvnI CGCG 2 cut(s) 374, 1178
MwoI GCNNNNNNNGC 3 cut(s) 77, 591, 859
NdeII GATC 9 cut(s) 277, 308, 436, 648, 1005, 1012, 1089, 1148, 1264
NheI GCTAGC 2 cut(s) 376, 896
NlaIII CATG 6 cut(s) 63, 467, 589, 700, 1067, 1189
NlaIV GGNNCC 3 cut(s) 490, 996, 1266
NmeAIII GCCGAG 1 cut(s) 561
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 1 cut(s) 63
NspV TTCGAA 1 cut(s) 164
PctI GAATGC 1 cut(s) 1125
PdmI GAANNNNTTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 300
PkrI GCNGC 4 cut(s) 725, 785, 960, 1020
PpuMI RGGWCCY 1 cut(s) 661
Psp5II RGGWCCY 1 cut(s) 661
Psp6I CCWGG 1 cut(s) 1129
PspGI CCWGG 1 cut(s) 1129
PspN4I GGNNCC 3 cut(s) 490, 996, 1266
PspPI GGNCC 5 cut(s) 560, 661, 770, 917, 1070
PspPPI RGGWCCY 1 cut(s) 661
PstI CTGCAG 1 cut(s) 1229
PstNI CAGNNNCTG 1 cut(s) 1021
PsuI RGATCY 3 cut(s) 277, 648, 1264
PvuII CAGCTG 1 cut(s) 1018
SaqAI TTAA 4 cut(s) 35, 596, 872, 911
SatI GCNGC 4 cut(s) 724, 784, 959, 1019
Sau3AI GATC 9 cut(s) 277, 308, 436, 648, 1005, 1012, 1089, 1148, 1264
Sau96I GGNCC 5 cut(s) 560, 661, 770, 917, 1070
ScrFI CCNGG 1 cut(s) 1131
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 3 cut(s) 658, 883, 1189
SfcI CTRYAG 1 cut(s) 1225
SfuI TTCGAA 1 cut(s) 164
SinI GGWCC 4 cut(s) 560, 661, 917, 1070
Sse9I AATT 5 cut(s) 254, 504, 619, 1024, 1051
SsiI CCGC 5 cut(s) 78, 173, 366, 573, 641
SspMI CTAG 7 cut(s) 96, 152, 377, 719, 863, 897, 944
StyD4I CCNGG 1 cut(s) 1129
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 2 cut(s) 341, 927
TaqI TCGA 2 cut(s) 164, 387
TasI AATT 5 cut(s) 254, 504, 619, 1024, 1051
Tru1I TTAA 4 cut(s) 35, 596, 872, 911
Tru9I TTAA 4 cut(s) 35, 596, 872, 911
TscAI CASTG 2 cut(s) 164, 817
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 4 cut(s) 723, 783, 958, 1018
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 4 cut(s) 602, 685, 1048, 1244
TspGWI ACGGA 1 cut(s) 909
TspRI CASTG 2 cut(s) 164, 817
Van91I CCANNNNNTGG 1 cut(s) 300
VpaK11BI GGWCC 4 cut(s) 560, 661, 917, 1070
XapI RAATTY 2 cut(s) 254, 1024
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 1 cut(s) 63
XmaJI CCTAGG 1 cut(s) 718
XmiI GTMKAC 2 cut(s) 237, 1219
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 7 cut(s) 96, 152, 377, 719, 863, 897, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.