Rh6BG487100

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0014329
Physical Location & Seq
Reverse (-)
69154406 .. 69157199
2794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG487100.1

Sequence Viewer

Length: 1335 bp
ATGAGAAGAAGCGCTTCAACCAAAGCTTTGTTTTTAACCTTCTTCTTCTTCCTCCTAACATGCCTCATTGCAAACTCTGCAACTCACAACATTACTAGAAGAAGTAGCAAAGACAAAACCCACAGAAAGAAGTGTCCTATAACCAGTAGTCTACTCCAGTGTTCGAAAACACCACCGCCACCGCCACCACCACCAGATTTACCAAACCCACAAAAAGTGCTCTCATTTGCAGACCAAAGGCTTCAAGTGGTATACCCAGTCATTCAAAAATTCAAGTCCATCATCACCTCAGATCCTCTCAACATCACCAAAACTTGGGTAGGATCAGATATATGCAGATACAAAGGCTTCTACTGTGACAATCCACCATACAACTTATCCGCAATCGCGCTAGCCTCCATCGACTTCAATGGCTTCCAACTCGGTGCTTCCTCCCTTGATGGGTTTCTTGATCAGCTCCCTGATATTGCTCTGTTCCATGCAAACTCCAACAACTTTTCCGGCACCATTTCCTCCAAAATTGCCAACCTCTGCTACCTCTATGAGCTTGACATAAGCAACAACTTATTCTCCGGTCCATTCCCAACCGCAGTTCTCGGCATGAACGGCTTAACTTTCTTGGACATAAGGTTCAATTTCTTCACCGGCTCTGTTCCGCCTCAGATTTTCACTCAGGACCTTGATGCTCTCTTCCTCAACAACAACAACTTCATGCAGAGCCTTCCTGATAACCTAGGCAGCTCCCATATAATCCTCCTCTCTTTGGCAAACAACAACTTCAATGGCCCTATTCCAAGCAGCATTGCAAAAGCTCTGTCTTCTCTCACTGAAGTTCTGCTTCTCAATAACCAGTTAACAGGTTGTTTGCCTTATGAGCTAGGTTTTTTAAAGGAGGCCATAGTGTTTGATGCTAGCAACAATCAGTTAACTGGTCCGTTACCGTTCTCTTTGGCTTGTCTAGACAAAGTGGAGCAGCTAAGTTTTGCTGGAAACTTATTGTTTGGCTTGGTGCCGGAGGTGATCTGTGATCAGCTGCTGAATTTGGCAAACTTTTCGCTGTCCAATAATTATTTCTTCCATGTTGGTCCAATATGTCAAAGTTTGATCCAGAGAGGACTGCTTGATGTTACTAACAACTGCATTCCTGGTCTTCCATTCCAAAGATCGGTAGAAGAATGTGCAGATTTCTTCGCGTATCCCATGTTCTGTCCGAATATGGGGTCTTATACCTATATTCCCTGCAAATGCCGTCCTCTGTTGAAATCCCAGAACCTTCAAGTGATAGCAGCAATGTCACTTTCTAGAGTGACCCTCTTTGCTTTCCAGAGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

49.06

Weight (kDa)

7.85

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 503, 1009
AccB7I CCANNNNNTGG 1 cut(s) 315
AccI GTMKAC 2 cut(s) 151, 252
AccII CGCG 2 cut(s) 389, 1193
AciI CCGC 5 cut(s) 176, 182, 381, 588, 656
AclWI GGATC 3 cut(s) 287, 331, 1099
AcsI RAATTY 2 cut(s) 269, 1039
AcuI CTGAAG 1 cut(s) 849
AfeI AGCGCT 1 cut(s) 13
AfiI CCNNNNNNNGG 5 cut(s) 315, 441, 763, 1165, 1217
AgsI TTSAA 9 cut(s) 18, 245, 266, 274, 409, 634, 781, 1261, 1277
AjnI CCWGG 1 cut(s) 1144
AjuI GAANNNNNNNTTGG 2 cut(s) 1055, 1087
AloI GAACNNNNNNTCC 2 cut(s) 614, 646
AluBI AGCT 8 cut(s) 26, 457, 547, 741, 812, 877, 976, 1033
AluI AGCT 8 cut(s) 26, 457, 547, 741, 812, 877, 976, 1033
Alw21I GWGCWC 1 cut(s) 222
AlwI GGATC 3 cut(s) 287, 331, 1099
AlwNI CAGNNNCTG 1 cut(s) 1036
Aor51HI AGCGCT 1 cut(s) 13
AoxI GGCC 2 cut(s) 784, 894
ApeKI GCWGC 5 cut(s) 738, 798, 973, 1033, 1286
ApoI RAATTY 2 cut(s) 269, 1039
ArsI GACNNNNNNTTYG 4 cut(s) 800, 832, 1205, 1237
Asp700I GAANNNNTTC 1 cut(s) 13
AspA2I CCTAGG 1 cut(s) 733
AspLEI GCGC 2 cut(s) 14, 391
AspS9I GGNCC 6 cut(s) 575, 676, 785, 932, 1085, 1329
AsuHPI GGTGA 4 cut(s) 277, 298, 634, 1030
AsuII TTCGAA 1 cut(s) 164
AsuNHI GCTAGC 2 cut(s) 391, 911
AvaII GGWCC 5 cut(s) 575, 676, 932, 1085, 1329
AvrII CCTAGG 1 cut(s) 733
BanI GGYRCC 2 cut(s) 503, 1009
BbsI GAAGAC 2 cut(s) 810, 1142
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 5 cut(s) 750, 810, 985, 1020, 1298
BccI CCATC 3 cut(s) 287, 407, 434
BceAI ACGGC 2 cut(s) 622, 1233
BcgI CGANNNNNNTGC 2 cut(s) 1035, 1069
BciT130I CCWGG 1 cut(s) 1146
BciVI GTATCC 1 cut(s) 1206
BclI TGATCA 2 cut(s) 451, 1027
BfaI CTAG 7 cut(s) 96, 392, 734, 878, 912, 959, 1302
BfoI RGCGCY 1 cut(s) 15
BfuI GTATCC 1 cut(s) 1206
BisI GCNGC 5 cut(s) 739, 799, 974, 1034, 1287
BlnI CCTAGG 1 cut(s) 733
BlsI GCNGC 5 cut(s) 740, 800, 975, 1035, 1288
Bme1390I CCNGG 1 cut(s) 1146
Bme18I GGWCC 5 cut(s) 575, 676, 932, 1085, 1329
BmgT120I GGNCC 6 cut(s) 575, 676, 785, 932, 1085, 1329
BmiI GGNNCC 2 cut(s) 505, 1011
BmrFI CCNGG 1 cut(s) 1146
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 2 cut(s) 673, 898
BmtI GCTAGC 2 cut(s) 395, 915
BmuI ACTGGG 1 cut(s) 251
BpiI GAAGAC 2 cut(s) 810, 1142
BplI GAGNNNNNCTC 2 cut(s) 1296, 1328
BpmI CTGGAG 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 733
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 5 cut(s) 315, 441, 763, 1165, 1217
Bse118I RCCGGY 1 cut(s) 644
Bse1I ACTGG 5 cut(s) 144, 157, 257, 850, 934
Bse3DI GCAATG 3 cut(s) 66, 801, 1296
BseBI CCWGG 1 cut(s) 1146
BseDI CCNNGG 1 cut(s) 733
BseLI CCNNNNNNNGG 5 cut(s) 315, 441, 763, 1165, 1217
BseMI GCAATG 3 cut(s) 66, 801, 1296
BseMII CTCAG 3 cut(s) 303, 674, 686
BseNI ACTGG 5 cut(s) 144, 157, 257, 850, 934
BseRI GAGGAG 1 cut(s) 746
BseXI GCAGC 5 cut(s) 750, 810, 985, 1020, 1298
BsgI GTGCAG 1 cut(s) 1200
Bsh1236I CGCG 2 cut(s) 389, 1193
BshFI GGCC 2 cut(s) 786, 896
BshNI GGYRCC 2 cut(s) 503, 1009
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 4 cut(s) 501, 573, 645, 1013
BslI CCNNNNNNNGG 5 cut(s) 315, 441, 763, 1165, 1217
BsmI GAATGC 1 cut(s) 1140
BsnI GGCC 2 cut(s) 786, 896
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 7 cut(s) 292, 323, 451, 1020, 1027, 1104, 1163
BspACI CCGC 5 cut(s) 176, 182, 381, 588, 656
BspANI GGCC 2 cut(s) 786, 896
BspCNI CTCAG 3 cut(s) 302, 673, 685
BspFNI CGCG 2 cut(s) 389, 1193
BspLI GGNNCC 2 cut(s) 505, 1011
BspOI GCTAGC 2 cut(s) 395, 915
BspPI GGATC 3 cut(s) 287, 331, 1099
BspT104I TTCGAA 1 cut(s) 164
BspT107I GGYRCC 2 cut(s) 503, 1009
BsrDI GCAATG 3 cut(s) 66, 801, 1296
BsrFI RCCGGY 1 cut(s) 644
BsrI ACTGG 5 cut(s) 144, 157, 257, 850, 934
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 1 cut(s) 733
BssMI GATC 7 cut(s) 292, 323, 451, 1020, 1027, 1104, 1163
BssNAI GTATAC 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 733
Bst1107I GTATAC 1 cut(s) 253
Bst2UI CCWGG 1 cut(s) 1146
Bst4CI ACNGT 2 cut(s) 356, 942
Bst6I CTCTTC 1 cut(s) 695
BstAPI GCANNNNNTGC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 393, 913
BstDEI CTNAG 4 cut(s) 289, 660, 672, 977
BstFNI CGCG 2 cut(s) 389, 1193
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 391
BstKTI GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
BstMBI GATC 7 cut(s) 292, 323, 451, 1020, 1027, 1104, 1163
BstMWI GCNNNNNNNGC 3 cut(s) 77, 606, 874
BstNI CCWGG 1 cut(s) 1146
BstNSI RCATGY 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 1144
BstUI CGCG 2 cut(s) 389, 1193
BstV1I GCAGC 5 cut(s) 750, 810, 985, 1020, 1298
BstV2I GAAGAC 2 cut(s) 810, 1142
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BstZ17I GTATAC 1 cut(s) 253
BsuI GTATCC 1 cut(s) 1206
BsuRI GGCC 2 cut(s) 786, 896
BtsIMutI CAGTG 2 cut(s) 164, 825
Cac8I GCNNGC 2 cut(s) 393, 913
CaiI CAGNNNCTG 1 cut(s) 1036
CfoI GCGC 2 cut(s) 14, 391
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 6 cut(s) 575, 676, 785, 932, 1085, 1329
CviAII CATG 6 cut(s) 60, 479, 601, 712, 1079, 1201
DdeI CTNAG 4 cut(s) 289, 660, 672, 977
DpnI GATC 7 cut(s) 294, 325, 453, 1022, 1029, 1106, 1165
DpnII GATC 7 cut(s) 292, 323, 451, 1020, 1027, 1104, 1163
DraI TTTAAA 1 cut(s) 888
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
EciI GGCGGA 1 cut(s) 645
Eco130I CCWWGG 1 cut(s) 733
Eco47I GGWCC 5 cut(s) 575, 676, 932, 1085, 1329
Eco47III AGCGCT 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 849
EcoO109I RGGNCCY 2 cut(s) 676, 1329
EcoRII CCWGG 1 cut(s) 1144
EcoT14I CCWWGG 1 cut(s) 733
ErhI CCWWGG 1 cut(s) 733
FaeI CATG 6 cut(s) 63, 482, 604, 715, 1082, 1204
FalI AAGNNNNNCTT 3 cut(s) 30, 822, 854
FatI CATG 6 cut(s) 59, 478, 600, 711, 1078, 1200
FbaI TGATCA 2 cut(s) 451, 1027
FblI GTMKAC 2 cut(s) 151, 252
Fnu4HI GCNGC 5 cut(s) 739, 799, 974, 1034, 1287
Fsp4HI GCNGC 5 cut(s) 739, 799, 974, 1034, 1287
FspBI CTAG 7 cut(s) 96, 392, 734, 878, 912, 959, 1302
GlaI GCGC 2 cut(s) 13, 390
GluI GCNGC 5 cut(s) 739, 799, 974, 1034, 1287
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 2 cut(s) 786, 896
HapII CCGG 4 cut(s) 501, 573, 645, 1013
HhaI GCGC 2 cut(s) 14, 391
Hin1II CATG 6 cut(s) 63, 482, 604, 715, 1082, 1204
Hin6I GCGC 2 cut(s) 12, 389
HinP1I GCGC 2 cut(s) 12, 389
HincII GTYRAC 2 cut(s) 855, 927
HindII GTYRAC 2 cut(s) 855, 927
HindIII AAGCTT 1 cut(s) 24
HpaI GTTAAC 2 cut(s) 855, 927
HpaII CCGG 4 cut(s) 501, 573, 645, 1013
HphI GGTGA 4 cut(s) 277, 298, 634, 1030
Hpy166II GTNNAC 4 cut(s) 152, 253, 855, 927
Hpy188I TCNGA 4 cut(s) 292, 328, 663, 1212
Hpy188III TCNNGA 7 cut(s) 449, 674, 725, 959, 1108, 1302, 1324
Hpy8I GTNNAC 4 cut(s) 152, 253, 855, 927
HpyAV CCTTC 3 cut(s) 49, 731, 1283
HpyCH4III ACNGT 2 cut(s) 356, 942
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 606, 874
HpyF3I CTNAG 4 cut(s) 289, 660, 672, 977
Hsp92II CATG 6 cut(s) 63, 482, 604, 715, 1082, 1204
HspAI GCGC 2 cut(s) 12, 389
Ksp22I TGATCA 2 cut(s) 451, 1027
KspAI GTTAAC 2 cut(s) 855, 927
Kzo9I GATC 7 cut(s) 292, 323, 451, 1020, 1027, 1104, 1163
LmnI GCTCC 3 cut(s) 462, 746, 970
Lsp1109I GCAGC 5 cut(s) 750, 810, 985, 1020, 1298
LweI GCATC 2 cut(s) 673, 898
MaeI CTAG 7 cut(s) 96, 392, 734, 878, 912, 959, 1302
MaeIII GTNAC 5 cut(s) 356, 936, 1126, 1293, 1306
MalI GATC 7 cut(s) 294, 325, 453, 1022, 1029, 1106, 1165
MboI GATC 7 cut(s) 292, 323, 451, 1020, 1027, 1104, 1163
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 5 cut(s) 269, 519, 634, 1039, 1066
MmeI TCCRAC 2 cut(s) 442, 513
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 5 cut(s) 35, 611, 854, 887, 926
MspA1I CMGCKG 1 cut(s) 1033
MspI CCGG 4 cut(s) 501, 573, 645, 1013
MspR9I CCNGG 1 cut(s) 1146
Mva1269I GAATGC 1 cut(s) 1140
MvaI CCWGG 1 cut(s) 1146
MvnI CGCG 2 cut(s) 389, 1193
MwoI GCNNNNNNNGC 3 cut(s) 77, 606, 874
NdeII GATC 7 cut(s) 292, 323, 451, 1020, 1027, 1104, 1163
NheI GCTAGC 2 cut(s) 391, 911
NlaIII CATG 6 cut(s) 63, 482, 604, 715, 1082, 1204
NlaIV GGNNCC 2 cut(s) 505, 1011
NmeAIII GCCGAG 1 cut(s) 576
NmuCI GTSAC 3 cut(s) 356, 1293, 1306
NspI RCATGY 1 cut(s) 63
NspV TTCGAA 1 cut(s) 164
PctI GAATGC 1 cut(s) 1140
PdmI GAANNNNTTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 315
PkrI GCNGC 5 cut(s) 740, 800, 975, 1035, 1288
PpuMI RGGWCCY 2 cut(s) 676, 1329
Psp5II RGGWCCY 2 cut(s) 676, 1329
Psp6I CCWGG 1 cut(s) 1144
PspGI CCWGG 1 cut(s) 1144
PspN4I GGNNCC 2 cut(s) 505, 1011
PspPI GGNCC 6 cut(s) 575, 676, 785, 932, 1085, 1329
PspPPI RGGWCCY 2 cut(s) 676, 1329
PsrI GAACNNNNNNTAC 2 cut(s) 1187, 1219
PstNI CAGNNNCTG 1 cut(s) 1036
PsuI RGATCY 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 1033
SaqAI TTAA 5 cut(s) 35, 611, 854, 887, 926
SatI GCNGC 5 cut(s) 739, 799, 974, 1034, 1287
Sau3AI GATC 7 cut(s) 292, 323, 451, 1020, 1027, 1104, 1163
Sau96I GGNCC 6 cut(s) 575, 676, 785, 932, 1085, 1329
ScrFI CCNGG 1 cut(s) 1146
SduI GDGCHC 1 cut(s) 222
SfaNI GCATC 2 cut(s) 673, 898
SfuI TTCGAA 1 cut(s) 164
SinI GGWCC 5 cut(s) 575, 676, 932, 1085, 1329
Sse9I AATT 5 cut(s) 269, 519, 634, 1039, 1066
SsiI CCGC 5 cut(s) 176, 182, 381, 588, 656
SspMI CTAG 7 cut(s) 96, 392, 734, 878, 912, 959, 1302
StyD4I CCNGG 1 cut(s) 1144
StyI CCWWGG 1 cut(s) 733
TaaI ACNGT 2 cut(s) 356, 942
TaqI TCGA 2 cut(s) 164, 402
TasI AATT 5 cut(s) 269, 519, 634, 1039, 1066
Tru1I TTAA 5 cut(s) 35, 611, 854, 887, 926
Tru9I TTAA 5 cut(s) 35, 611, 854, 887, 926
TscAI CASTG 2 cut(s) 164, 832
TseFI GTSAC 3 cut(s) 356, 1293, 1306
TseI GCWGC 5 cut(s) 738, 798, 973, 1033, 1286
Tsp45I GTSAC 3 cut(s) 356, 1293, 1306
TspDTI ATGAA 2 cut(s) 617, 700
TspGWI ACGGA 1 cut(s) 924
TspRI CASTG 2 cut(s) 164, 832
Van91I CCANNNNNTGG 1 cut(s) 315
VpaK11BI GGWCC 5 cut(s) 575, 676, 932, 1085, 1329
XapI RAATTY 2 cut(s) 269, 1039
XbaI TCTAGA 2 cut(s) 958, 1301
XceI RCATGY 1 cut(s) 63
XmaJI CCTAGG 1 cut(s) 733
XmiI GTMKAC 2 cut(s) 151, 252
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 7 cut(s) 96, 392, 734, 878, 912, 959, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.