Rh6AG476900

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014329
Physical Location & Seq
Reverse (-)
65833379 .. 65835863
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG476900.1

Sequence Viewer

Length: 1338 bp
ATGAGAAGAAGCGCTTCAACCAAAGCGTTGTTTTTAACCTTCTTCTTCTTCTTCCTCCTAACATGCCTCATTGCAAACTCTGCGACTCACAACATTACTAGAAGAAGTAGCAAAGACAAAACCCACAGAAAGAAGTGTCCTATAACCAGTAGTCTACTCCAGTGTTCGAAAACACCACCGCCACCACCACCACCACCAGATGCACCAAACCCACAAAAAGTGCTCTCATTTGCAGACCAAAGGCTTCAAGTGGTATACCCAGTCATTCAAAAATTCAAGTCCATCATCACCTCAGATCCTCTCAACATCACCAAAACTTGGGTAGGATCAGATATATGCAAATACAAAGGCTTCTACTGTGACAATCCACCATACAACTTATCTGCAATCGCGCTAGCCTCCATCGACTTCAATGGCTTCCAACTCGGTGCTTCCTCCCTTGATGGGTTTCTTGATCAGCTCCCTGATATTGCTCTGTTCCATGCAAACTCCAACAACTTTTCCGGCACCATTTCCTCCAAAATTGCCAACCTCCCCTACCTCTATGAGCTTGACATTAGCAACAACTTATTCTCCGGTCCATTCCCAACCGCAGTTCTCGGCATGAACGGCTTAACTTTCTTGGACATAAGGTTCAATTTCTTCACCGGCTCTGTTCCGCCTCAGATTTTCACTCAGGACCTTGATGCTCTCTTCCTCAACAACAACAACTTCATGCAGAGCCTTCCGGATAACCTAGGCAGCTCCCATATAATTCTCCTCACTTTGGCAAACAACAACTTCAATGGCCCTATTCCAAGCAGCATTGCAAAAGCTCTGTCTTCTCTCACTGAAGTTCTGCTTCTCAATAACCAGTTAACAGGTTGTTTGCCTTATGAGCTAGGTTTTTTAAAGGAGGCCATAGTGTTTGATGCTAGCAACAATCAGTTAACTGGTCCGTTACCGTTCTCTTTGGCTTGTCTAGACAAAGTGGAGCAGCTAAGTTTTGCTGGAAACTTATTGTTTGGCTTGGTGCCGGAGGTGATCTGTGATCAGCTGCTGAATCTGGCAAACTTTTCGCTGTCCAATAATTACTTCATCCATGTTGGTCCAATATGTCAAAGTTTGATCCAGAGAGGACTGCTTGATGTTACTAATAACTGCATTCCTGGTCTTCCATTCCAAAGATCGGTAGAAGAATGTGCAGATTTCTTCGCGCATCCCATGTTCTGTCCGAATATGGGGTCTTATACCTGTATTCCCTGCAAATGCCGTCCTCTGTTGAAATCCCAGAACCTTCAAGTGATAGCAGCAATGTCACTTTCTAGAGTGACCCTCTTTGCTTTCCAGAGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

445

Amino Acids

49.03

Weight (kDa)

7.86

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 506, 1012
AccB7I CCANNNNNTGG 1 cut(s) 318
AccI GTMKAC 2 cut(s) 154, 255
AccII CGCG 2 cut(s) 392, 1196
AccIII TCCGGA 1 cut(s) 727
AciI CCGC 3 cut(s) 179, 591, 659
AclWI GGATC 3 cut(s) 290, 334, 1102
AcsI RAATTY 1 cut(s) 272
AcuI CTGAAG 1 cut(s) 852
AfeI AGCGCT 1 cut(s) 13
AfiI CCNNNNNNNGG 5 cut(s) 318, 444, 766, 1168, 1220
AgsI TTSAA 9 cut(s) 18, 248, 269, 277, 412, 637, 784, 1264, 1280
AjnI CCWGG 1 cut(s) 1147
AjuI GAANNNNNNNTTGG 2 cut(s) 1058, 1090
AloI GAACNNNNNNTCC 2 cut(s) 617, 649
AluBI AGCT 7 cut(s) 460, 550, 744, 815, 880, 979, 1036
AluI AGCT 7 cut(s) 460, 550, 744, 815, 880, 979, 1036
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 3 cut(s) 290, 334, 1102
AlwNI CAGNNNCTG 1 cut(s) 1039
Aor13HI TCCGGA 1 cut(s) 727
Aor51HI AGCGCT 1 cut(s) 13
AoxI GGCC 2 cut(s) 787, 897
ApeKI GCWGC 5 cut(s) 741, 801, 976, 1036, 1289
ApoI RAATTY 1 cut(s) 272
ArsI GACNNNNNNTTYG 4 cut(s) 803, 835, 1208, 1240
Asp700I GAANNNNTTC 1 cut(s) 13
AspA2I CCTAGG 1 cut(s) 736
AspLEI GCGC 3 cut(s) 14, 394, 1198
AspS9I GGNCC 6 cut(s) 578, 679, 788, 935, 1088, 1332
AsuHPI GGTGA 4 cut(s) 280, 301, 637, 1033
AsuII TTCGAA 1 cut(s) 167
AsuNHI GCTAGC 2 cut(s) 394, 914
AvaII GGWCC 5 cut(s) 578, 679, 935, 1088, 1332
AvrII CCTAGG 1 cut(s) 736
BanI GGYRCC 2 cut(s) 506, 1012
BbsI GAAGAC 2 cut(s) 813, 1145
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 5 cut(s) 753, 813, 988, 1023, 1301
BccI CCATC 3 cut(s) 290, 410, 437
BceAI ACGGC 2 cut(s) 625, 1236
BcgI CGANNNNNNTGC 2 cut(s) 1038, 1072
BciT130I CCWGG 1 cut(s) 1149
BclI TGATCA 2 cut(s) 454, 1030
BfaI CTAG 7 cut(s) 99, 395, 737, 881, 915, 962, 1305
BfoI RGCGCY 1 cut(s) 15
BisI GCNGC 5 cut(s) 742, 802, 977, 1037, 1290
BlnI CCTAGG 1 cut(s) 736
BlsI GCNGC 5 cut(s) 743, 803, 978, 1038, 1291
Bme1390I CCNGG 1 cut(s) 1149
Bme18I GGWCC 5 cut(s) 578, 679, 935, 1088, 1332
BmgT120I GGNCC 6 cut(s) 578, 679, 788, 935, 1088, 1332
BmiI GGNNCC 2 cut(s) 508, 1014
BmrFI CCNGG 1 cut(s) 1149
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 4 cut(s) 190, 676, 901, 1207
BmtI GCTAGC 2 cut(s) 398, 918
BmuI ACTGGG 1 cut(s) 254
BpiI GAAGAC 2 cut(s) 813, 1145
BplI GAGNNNNNCTC 2 cut(s) 1299, 1331
BpmI CTGGAG 1 cut(s) 143
Bpu14I TTCGAA 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 736
BsaWI WCCGGW 2 cut(s) 575, 727
BsaXI ACNNNNNCTCC 2 cut(s) 557, 587
Bsc4I CCNNNNNNNGG 5 cut(s) 318, 444, 766, 1168, 1220
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 5 cut(s) 147, 160, 260, 853, 937
Bse3DI GCAATG 3 cut(s) 69, 804, 1299
BseAI TCCGGA 1 cut(s) 727
BseBI CCWGG 1 cut(s) 1149
BseDI CCNNGG 1 cut(s) 736
BseGI GGATG 2 cut(s) 1077, 1198
BseLI CCNNNNNNNGG 5 cut(s) 318, 444, 766, 1168, 1220
BseMI GCAATG 3 cut(s) 69, 804, 1299
BseMII CTCAG 3 cut(s) 306, 677, 689
BseNI ACTGG 5 cut(s) 147, 160, 260, 853, 937
BseRI GAGGAG 1 cut(s) 749
BseXI GCAGC 5 cut(s) 753, 813, 988, 1023, 1301
BsgI GTGCAG 1 cut(s) 1203
Bsh1236I CGCG 2 cut(s) 392, 1196
BshFI GGCC 2 cut(s) 789, 899
BshNI GGYRCC 2 cut(s) 506, 1012
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 5 cut(s) 504, 576, 648, 728, 1016
BslI CCNNNNNNNGG 5 cut(s) 318, 444, 766, 1168, 1220
BsmI GAATGC 1 cut(s) 1143
BsnI GGCC 2 cut(s) 789, 899
Bsp119I TTCGAA 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 225
Bsp13I TCCGGA 1 cut(s) 727
Bsp143I GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
BspACI CCGC 3 cut(s) 179, 591, 659
BspANI GGCC 2 cut(s) 789, 899
BspCNI CTCAG 3 cut(s) 305, 676, 688
BspEI TCCGGA 1 cut(s) 727
BspFNI CGCG 2 cut(s) 392, 1196
BspLI GGNNCC 2 cut(s) 508, 1014
BspOI GCTAGC 2 cut(s) 398, 918
BspPI GGATC 3 cut(s) 290, 334, 1102
BspT104I TTCGAA 1 cut(s) 167
BspT107I GGYRCC 2 cut(s) 506, 1012
BsrDI GCAATG 3 cut(s) 69, 804, 1299
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 5 cut(s) 147, 160, 260, 853, 937
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 1 cut(s) 736
BssMI GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
BssNAI GTATAC 1 cut(s) 256
BssT1I CCWWGG 1 cut(s) 736
Bst1107I GTATAC 1 cut(s) 256
Bst2UI CCWGG 1 cut(s) 1149
Bst4CI ACNGT 2 cut(s) 359, 945
Bst6I CTCTTC 1 cut(s) 698
BstAPI GCANNNNNTGC 1 cut(s) 80
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 396, 916
BstDEI CTNAG 4 cut(s) 292, 663, 675, 980
BstF5I GGATG 2 cut(s) 1077, 1198
BstFNI CGCG 2 cut(s) 392, 1196
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 3 cut(s) 14, 394, 1198
BstKTI GATC 7 cut(s) 298, 329, 457, 1026, 1033, 1110, 1169
BstMBI GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
BstMWI GCNNNNNNNGC 3 cut(s) 80, 609, 877
BstNI CCWGG 1 cut(s) 1149
BstNSI RCATGY 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 1147
BstUI CGCG 2 cut(s) 392, 1196
BstV1I GCAGC 5 cut(s) 753, 813, 988, 1023, 1301
BstV2I GAAGAC 2 cut(s) 813, 1145
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZ17I GTATAC 1 cut(s) 256
BsuRI GGCC 2 cut(s) 789, 899
BtsCI GGATG 2 cut(s) 1077, 1198
BtsIMutI CAGTG 2 cut(s) 167, 828
Cac8I GCNNGC 2 cut(s) 396, 916
CaiI CAGNNNCTG 1 cut(s) 1039
CfoI GCGC 3 cut(s) 14, 394, 1198
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 6 cut(s) 578, 679, 788, 935, 1088, 1332
CviAII CATG 6 cut(s) 63, 482, 604, 715, 1082, 1204
DdeI CTNAG 4 cut(s) 292, 663, 675, 980
DpnI GATC 7 cut(s) 297, 328, 456, 1025, 1032, 1109, 1168
DpnII GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
DraI TTTAAA 1 cut(s) 891
Eam1104I CTCTTC 1 cut(s) 698
EarI CTCTTC 1 cut(s) 698
EciI GGCGGA 1 cut(s) 648
Eco130I CCWWGG 1 cut(s) 736
Eco47I GGWCC 5 cut(s) 578, 679, 935, 1088, 1332
Eco47III AGCGCT 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 852
EcoO109I RGGNCCY 2 cut(s) 679, 1332
EcoRII CCWGG 1 cut(s) 1147
EcoT14I CCWWGG 1 cut(s) 736
ErhI CCWWGG 1 cut(s) 736
FaeI CATG 6 cut(s) 66, 485, 607, 718, 1085, 1207
FalI AAGNNNNNCTT 3 cut(s) 30, 825, 857
FatI CATG 6 cut(s) 62, 481, 603, 714, 1081, 1203
FbaI TGATCA 2 cut(s) 454, 1030
FblI GTMKAC 2 cut(s) 154, 255
Fnu4HI GCNGC 5 cut(s) 742, 802, 977, 1037, 1290
FokI GGATG 2 cut(s) 1064, 1185
Fsp4HI GCNGC 5 cut(s) 742, 802, 977, 1037, 1290
FspBI CTAG 7 cut(s) 99, 395, 737, 881, 915, 962, 1305
GlaI GCGC 3 cut(s) 13, 393, 1197
GluI GCNGC 5 cut(s) 742, 802, 977, 1037, 1290
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 2 cut(s) 789, 899
HapII CCGG 5 cut(s) 504, 576, 648, 728, 1016
HhaI GCGC 3 cut(s) 14, 394, 1198
Hin1II CATG 6 cut(s) 66, 485, 607, 718, 1085, 1207
Hin6I GCGC 3 cut(s) 12, 392, 1196
HinP1I GCGC 3 cut(s) 12, 392, 1196
HincII GTYRAC 2 cut(s) 858, 930
HindII GTYRAC 2 cut(s) 858, 930
HinfI GANTC 2 cut(s) 85, 1042
HpaI GTTAAC 2 cut(s) 858, 930
HpaII CCGG 5 cut(s) 504, 576, 648, 728, 1016
HphI GGTGA 4 cut(s) 280, 301, 637, 1033
Hpy166II GTNNAC 4 cut(s) 155, 256, 858, 930
Hpy188I TCNGA 4 cut(s) 295, 331, 666, 1215
Hpy188III TCNNGA 7 cut(s) 452, 677, 728, 962, 1111, 1305, 1327
Hpy8I GTNNAC 4 cut(s) 155, 256, 858, 930
HpyAV CCTTC 3 cut(s) 49, 734, 1286
HpyCH4III ACNGT 2 cut(s) 359, 945
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 609, 877
HpyF3I CTNAG 4 cut(s) 292, 663, 675, 980
Hsp92II CATG 6 cut(s) 66, 485, 607, 718, 1085, 1207
HspAI GCGC 3 cut(s) 12, 392, 1196
Kpn2I TCCGGA 1 cut(s) 727
Ksp22I TGATCA 2 cut(s) 454, 1030
KspAI GTTAAC 2 cut(s) 858, 930
Kzo9I GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
LmnI GCTCC 3 cut(s) 465, 749, 973
Lsp1109I GCAGC 5 cut(s) 753, 813, 988, 1023, 1301
LweI GCATC 4 cut(s) 190, 676, 901, 1207
MaeI CTAG 7 cut(s) 99, 395, 737, 881, 915, 962, 1305
MaeIII GTNAC 5 cut(s) 359, 939, 1129, 1296, 1309
MalI GATC 7 cut(s) 297, 328, 456, 1025, 1032, 1109, 1168
MboI GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 5 cut(s) 272, 522, 637, 753, 1069
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 2 cut(s) 445, 516
MroI TCCGGA 1 cut(s) 727
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 5 cut(s) 35, 614, 857, 890, 929
MspA1I CMGCKG 1 cut(s) 1036
MspI CCGG 5 cut(s) 504, 576, 648, 728, 1016
MspR9I CCNGG 1 cut(s) 1149
Mva1269I GAATGC 1 cut(s) 1143
MvaI CCWGG 1 cut(s) 1149
MvnI CGCG 2 cut(s) 392, 1196
MwoI GCNNNNNNNGC 3 cut(s) 80, 609, 877
NdeII GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
NheI GCTAGC 2 cut(s) 394, 914
NlaIII CATG 6 cut(s) 66, 485, 607, 718, 1085, 1207
NlaIV GGNNCC 2 cut(s) 508, 1014
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 3 cut(s) 359, 1296, 1309
NspI RCATGY 1 cut(s) 66
NspV TTCGAA 1 cut(s) 167
PctI GAATGC 1 cut(s) 1143
PdmI GAANNNNTTC 1 cut(s) 13
PfeI GAWTC 1 cut(s) 1042
PflMI CCANNNNNTGG 1 cut(s) 318
PkrI GCNGC 5 cut(s) 743, 803, 978, 1038, 1291
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
PpuMI RGGWCCY 2 cut(s) 679, 1332
Psp5II RGGWCCY 2 cut(s) 679, 1332
Psp6I CCWGG 1 cut(s) 1147
PspGI CCWGG 1 cut(s) 1147
PspN4I GGNNCC 2 cut(s) 508, 1014
PspPI GGNCC 6 cut(s) 578, 679, 788, 935, 1088, 1332
PspPPI RGGWCCY 2 cut(s) 679, 1332
PstNI CAGNNNCTG 1 cut(s) 1039
PsuI RGATCY 1 cut(s) 295
PvuII CAGCTG 1 cut(s) 1036
SaqAI TTAA 5 cut(s) 35, 614, 857, 890, 929
SatI GCNGC 5 cut(s) 742, 802, 977, 1037, 1290
Sau3AI GATC 7 cut(s) 295, 326, 454, 1023, 1030, 1107, 1166
Sau96I GGNCC 6 cut(s) 578, 679, 788, 935, 1088, 1332
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 1 cut(s) 1149
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 4 cut(s) 190, 676, 901, 1207
SfuI TTCGAA 1 cut(s) 167
SinI GGWCC 5 cut(s) 578, 679, 935, 1088, 1332
Sse9I AATT 5 cut(s) 272, 522, 637, 753, 1069
SsiI CCGC 3 cut(s) 179, 591, 659
SspMI CTAG 7 cut(s) 99, 395, 737, 881, 915, 962, 1305
StyD4I CCNGG 1 cut(s) 1147
StyI CCWWGG 1 cut(s) 736
TaaI ACNGT 2 cut(s) 359, 945
TaqI TCGA 2 cut(s) 167, 405
TasI AATT 5 cut(s) 272, 522, 637, 753, 1069
TfiI GAWTC 1 cut(s) 1042
Tru1I TTAA 5 cut(s) 35, 614, 857, 890, 929
Tru9I TTAA 5 cut(s) 35, 614, 857, 890, 929
TscAI CASTG 2 cut(s) 167, 835
TseFI GTSAC 3 cut(s) 359, 1296, 1309
TseI GCWGC 5 cut(s) 741, 801, 976, 1036, 1289
Tsp45I GTSAC 3 cut(s) 359, 1296, 1309
TspDTI ATGAA 3 cut(s) 620, 703, 1066
TspGWI ACGGA 1 cut(s) 927
TspRI CASTG 2 cut(s) 167, 835
Van91I CCANNNNNTGG 1 cut(s) 318
VpaK11BI GGWCC 5 cut(s) 578, 679, 935, 1088, 1332
XapI RAATTY 1 cut(s) 272
XbaI TCTAGA 2 cut(s) 961, 1304
XceI RCATGY 1 cut(s) 66
XmaJI CCTAGG 1 cut(s) 736
XmiI GTMKAC 2 cut(s) 154, 255
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 7 cut(s) 99, 395, 737, 881, 915, 962, 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.