FvH4_2g33930

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014329
Physical Location & Seq
Forward (+)
25314396 .. 25315716
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33930.t1

Sequence Viewer

Length: 1293 bp
ATGAGAAGAAGCAGTTTAACCAAAGCTTGTTTCTCTACCTTCTTCTTCTTCTTCCTCCTTACATGCCTCATTGCAAACTCTGCGACTCACAACATTACCAGAAGAAGTAGCAAAGACAAAACCCATAGAAAGAAGTGTCCTATAACTAGTAGTACACACCAGTGTTCAATACCAACACCACCACCACCCCCAGCTTTACCTCCAAACACACAAAAGGTTCTCTCATTTGCAGACCAAAGGCTTCAAGTGGTATACCCAGTCATCCAGAAATTCAAGTCCATCATCACCTCTGATCCTCTCAACATCACCAAAAGTTGGGTAGGATCAGATATCTGCAACTACAAAGGCTTCTACTGTGACAATCCACCATACAACTCATCCGCAATCGCGCTTGCCTCAATCGACTTTAATGGCTTCCAACTCGGTGCTTCTTCCCTTGATGGTTTTCTTGATCAGCTTCCTGATATTGCTCTGTTCCATGCAAACTCCAACAACTTTTCCGGCACCATTTCCTCCAAAATTGCCAACCTCCCCTATCTCTATGAGCTTGACATAAGCAACAACTTATTCTCTGGTTCATTCCCAACCGCAGTTCTTGGCATGAACAGCTTATCTTTCTTGGACCTAAGGTTCAATTTCTTTACAGGTTCTGTTCCGCCTCAGATTTTCACTCAGGACCTTGATGCTCTCTTCCTCAACAACAACAACTTCATGCAGAGCCTCCCTGATAATCTAGGCAGCTCACATATTATCCTCCTCACTTTGGCAAACAACAACTTCAATGGCCCTATTCCAACCAGCATTGCAGCAGCTTTGTCATCTCTCACTGAAGTCCTGCTACTCAATAACCAGCTCACAGGTTGTTTGCCTTACGAACTTGGTTTACTAAAGGAGGCCATAGTTCTTGATGCTAGCAACAATCAGTTAACTGGTCCATTACCGTTCTCTTTGGCTTGCCTAGACAAAGTTGAGCAGCTGAGTTTTGCTGGCAACTTATTGTTTGGCTTGGTGCCGGATGTGATCTGTGAGCTGCTGGTGAATTTGGCAAACTTCTCGCTGTCCGATAATTACTTCATCCATGTTGGTCCAATATGTCAACGTTTGATTCAGAGAGGACTGCTGGATGTTACTAACAACTGCATTCCTGGTCTTCCATTCCAAAGATCAGTAGAAGAATGTGCAGGTTTCTTTGCGCATCCCAGGTTCTGTCCCAATATGTGGTCCTATACCTACATTCCCTGCAGTAAGTTTCCTTATACTTCTTTGGTTCATCAAGTGAATCCTACACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

431

Amino Acids

47.37

Weight (kDa)

6.43

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1194
Acc36I ACCTGC 1 cut(s) 1172
AccB1I GGYRCC 2 cut(s) 503, 1009
AccB7I CCANNNNNTGG 2 cut(s) 315, 1218
AccI GTMKAC 1 cut(s) 252
AccII CGCG 1 cut(s) 389
AciI CCGC 3 cut(s) 381, 588, 656
AclI AACGTT 1 cut(s) 1099
AclWI GGATC 2 cut(s) 287, 331
AcsI RAATTY 2 cut(s) 269, 1039
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 1 cut(s) 154
AfiI CCNNNNNNNGG 3 cut(s) 315, 763, 1218
AgsI TTSAA 5 cut(s) 168, 245, 274, 634, 781
AhlI ACTAGT 1 cut(s) 146
AjnI CCWGG 2 cut(s) 1144, 1199
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AleI CACNNNNGTG 1 cut(s) 160
AloI GAACNNNNNNTCC 2 cut(s) 614, 646
AlwI GGATC 2 cut(s) 287, 331
AlwNI CAGNNNCTG 2 cut(s) 650, 1206
AoxI GGCC 2 cut(s) 784, 894
ApeKI GCWGC 5 cut(s) 738, 806, 809, 973, 1030
ApoI RAATTY 2 cut(s) 269, 1039
AspLEI GCGC 2 cut(s) 391, 1195
AspS9I GGNCC 6 cut(s) 622, 676, 785, 932, 1085, 1221
AsuHPI GGTGA 3 cut(s) 277, 298, 1048
AsuNHI GCTAGC 1 cut(s) 911
AvaII GGWCC 5 cut(s) 622, 676, 932, 1085, 1221
AxyI CCTNAGG 1 cut(s) 626
BanI GGYRCC 2 cut(s) 503, 1009
BarI GAAGNNNNNNTAC 2 cut(s) 822, 854
BbsI GAAGAC 1 cut(s) 1142
BbvI GCAGC 5 cut(s) 750, 818, 821, 985, 1017
BccI CCATC 2 cut(s) 287, 434
BcgI CGANNNNNNTGC 2 cut(s) 1035, 1069
BciT130I CCWGG 2 cut(s) 1146, 1201
BclI TGATCA 1 cut(s) 451
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 4 cut(s) 147, 734, 912, 959
BfmI CTRYAG 1 cut(s) 1240
BfuAI ACCTGC 1 cut(s) 1172
BisI GCNGC 5 cut(s) 739, 807, 810, 974, 1031
BlsI GCNGC 5 cut(s) 740, 808, 811, 975, 1032
Bme1390I CCNGG 2 cut(s) 1146, 1201
Bme18I GGWCC 5 cut(s) 622, 676, 932, 1085, 1221
BmgT120I GGNCC 6 cut(s) 622, 676, 785, 932, 1085, 1221
BmiI GGNNCC 2 cut(s) 505, 1011
BmrFI CCNGG 2 cut(s) 1146, 1201
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 3 cut(s) 673, 898, 1204
BmtI GCTAGC 1 cut(s) 915
BmuI ACTGGG 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 1142
BsaJI CCNNGG 1 cut(s) 1199
Bsc4I CCNNNNNNNGG 3 cut(s) 315, 763, 1218
Bse1I ACTGG 3 cut(s) 160, 257, 934
Bse21I CCTNAGG 1 cut(s) 626
Bse3DI GCAATG 2 cut(s) 69, 801
BseBI CCWGG 2 cut(s) 1146, 1201
BseDI CCNNGG 1 cut(s) 1199
BseGI GGATG 6 cut(s) 261, 377, 1021, 1074, 1129, 1195
BseLI CCNNNNNNNGG 3 cut(s) 315, 763, 1218
BseMI GCAATG 2 cut(s) 69, 801
BseMII CTCAG 3 cut(s) 674, 686, 968
BseNI ACTGG 3 cut(s) 160, 257, 934
BseRI GAGGAG 1 cut(s) 746
BseXI GCAGC 5 cut(s) 750, 818, 821, 985, 1017
BseYI CCCAGC 1 cut(s) 190
BsgI GTGCAG 1 cut(s) 1200
Bsh1236I CGCG 1 cut(s) 389
BshFI GGCC 2 cut(s) 786, 896
BshNI GGYRCC 2 cut(s) 503, 1009
BsiSI CCGG 2 cut(s) 501, 1013
BslFI GGGAC 1 cut(s) 1194
BslI CCNNNNNNNGG 3 cut(s) 315, 763, 1218
BsmFI GGGAC 1 cut(s) 1194
BsmI GAATGC 1 cut(s) 1140
BsnI GGCC 2 cut(s) 786, 896
Bsp143I GATC 5 cut(s) 292, 323, 451, 1020, 1163
BspACI CCGC 3 cut(s) 381, 588, 656
BspANI GGCC 2 cut(s) 786, 896
BspCNI CTCAG 3 cut(s) 673, 685, 969
BspFNI CGCG 1 cut(s) 389
BspLI GGNNCC 2 cut(s) 505, 1011
BspMAI CTGCAG 1 cut(s) 1244
BspMI ACCTGC 1 cut(s) 1172
BspOI GCTAGC 1 cut(s) 915
BspPI GGATC 2 cut(s) 287, 331
BspT107I GGYRCC 2 cut(s) 503, 1009
BsrDI GCAATG 2 cut(s) 69, 801
BsrI ACTGG 3 cut(s) 160, 257, 934
BssECI CCNNGG 1 cut(s) 1199
BssMI GATC 5 cut(s) 292, 323, 451, 1020, 1163
BssNAI GTATAC 1 cut(s) 253
Bst1107I GTATAC 1 cut(s) 253
Bst2UI CCWGG 2 cut(s) 1146, 1201
Bst4CI ACNGT 2 cut(s) 356, 942
Bst6I CTCTTC 1 cut(s) 695
BstAPI GCANNNNNTGC 1 cut(s) 80
BstC8I GCNNGC 4 cut(s) 393, 913, 955, 988
BstDEI CTNAG 4 cut(s) 626, 660, 672, 977
BstF5I GGATG 6 cut(s) 261, 377, 1021, 1074, 1129, 1195
BstFNI CGCG 1 cut(s) 389
BstHHI GCGC 2 cut(s) 391, 1195
BstKTI GATC 5 cut(s) 295, 326, 454, 1023, 1166
BstMBI GATC 5 cut(s) 292, 323, 451, 1020, 1163
BstMWI GCNNNNNNNGC 2 cut(s) 80, 606
BstNI CCWGG 2 cut(s) 1146, 1201
BstNSI RCATGY 1 cut(s) 66
BstSCI CCNGG 2 cut(s) 1144, 1199
BstSFI CTRYAG 1 cut(s) 1240
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 5 cut(s) 750, 818, 821, 985, 1017
BstV2I GAAGAC 1 cut(s) 1142
BstZ17I GTATAC 1 cut(s) 253
Bsu36I CCTNAGG 1 cut(s) 626
BsuRI GGCC 2 cut(s) 786, 896
BtsCI GGATG 6 cut(s) 261, 377, 1021, 1074, 1129, 1195
BtsIMutI CAGTG 2 cut(s) 167, 825
BveI ACCTGC 1 cut(s) 1172
Cac8I GCNNGC 4 cut(s) 393, 913, 955, 988
CaiI CAGNNNCTG 2 cut(s) 650, 1206
CfoI GCGC 2 cut(s) 391, 1195
Cfr13I GGNCC 6 cut(s) 622, 676, 785, 932, 1085, 1221
Csp6I GTAC 1 cut(s) 153
CviAII CATG 5 cut(s) 63, 479, 601, 712, 1079
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 4 cut(s) 626, 660, 672, 977
DpnI GATC 5 cut(s) 294, 325, 453, 1022, 1165
DpnII GATC 5 cut(s) 292, 323, 451, 1020, 1163
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
EciI GGCGGA 1 cut(s) 645
Eco32I GATATC 1 cut(s) 331
Eco47I GGWCC 5 cut(s) 622, 676, 932, 1085, 1221
Eco57I CTGAAG 1 cut(s) 849
Eco81I CCTNAGG 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 2 cut(s) 1144, 1199
EcoRV GATATC 1 cut(s) 331
FaeI CATG 5 cut(s) 66, 482, 604, 715, 1082
FaqI GGGAC 1 cut(s) 1194
FatI CATG 5 cut(s) 62, 478, 600, 711, 1078
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 5 cut(s) 739, 807, 810, 974, 1031
FokI GGATG 6 cut(s) 248, 364, 1028, 1061, 1136, 1182
Fsp4HI GCNGC 5 cut(s) 739, 807, 810, 974, 1031
FspBI CTAG 4 cut(s) 147, 734, 912, 959
FspI TGCGCA 1 cut(s) 1194
GlaI GCGC 2 cut(s) 390, 1194
GluI GCNGC 5 cut(s) 739, 807, 810, 974, 1031
GsaI CCCAGC 1 cut(s) 194
HaeIII GGCC 2 cut(s) 786, 896
HapII CCGG 2 cut(s) 501, 1013
HhaI GCGC 2 cut(s) 391, 1195
Hin1II CATG 5 cut(s) 66, 482, 604, 715, 1082
Hin6I GCGC 2 cut(s) 389, 1193
HinP1I GCGC 2 cut(s) 389, 1193
HincII GTYRAC 2 cut(s) 927, 1097
HindII GTYRAC 2 cut(s) 927, 1097
HindIII AAGCTT 1 cut(s) 24
HinfI GANTC 3 cut(s) 85, 1105, 1279
HpaI GTTAAC 1 cut(s) 927
HpaII CCGG 2 cut(s) 501, 1013
HphI GGTGA 3 cut(s) 277, 298, 1048
Hpy166II GTNNAC 5 cut(s) 155, 253, 884, 927, 1097
Hpy188I TCNGA 5 cut(s) 292, 328, 663, 1063, 1110
Hpy188III TCNNGA 5 cut(s) 265, 449, 461, 674, 905
Hpy8I GTNNAC 5 cut(s) 155, 253, 884, 927, 1097
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 2 cut(s) 356, 942
HpyCH4IV ACGT 1 cut(s) 1099
HpyCH4V TGCA 9 cut(s) 74, 230, 336, 482, 715, 806, 1140, 1181, 1242
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 606
HpyF3I CTNAG 4 cut(s) 626, 660, 672, 977
HpySE526I ACGT 1 cut(s) 1099
Hsp92II CATG 5 cut(s) 66, 482, 604, 715, 1082
HspAI GCGC 2 cut(s) 389, 1193
Ksp22I TGATCA 1 cut(s) 451
KspAI GTTAAC 1 cut(s) 927
Kzo9I GATC 5 cut(s) 292, 323, 451, 1020, 1163
Lsp1109I GCAGC 5 cut(s) 750, 818, 821, 985, 1017
LweI GCATC 3 cut(s) 673, 898, 1204
MaeI CTAG 4 cut(s) 147, 734, 912, 959
MaeII ACGT 1 cut(s) 1099
MaeIII GTNAC 2 cut(s) 356, 1126
MalI GATC 5 cut(s) 294, 325, 453, 1022, 1165
MboI GATC 5 cut(s) 292, 323, 451, 1020, 1163
MluCI AATT 5 cut(s) 269, 519, 634, 1039, 1066
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 3 cut(s) 442, 513, 818
MseI TTAA 3 cut(s) 17, 408, 926
MslI CAYNNNNRTG 1 cut(s) 160
MspA1I CMGCKG 1 cut(s) 976
MspI CCGG 2 cut(s) 501, 1013
MspR9I CCNGG 2 cut(s) 1146, 1201
Mva1269I GAATGC 1 cut(s) 1140
MvaI CCWGG 2 cut(s) 1146, 1201
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 2 cut(s) 80, 606
NdeII GATC 5 cut(s) 292, 323, 451, 1020, 1163
NheI GCTAGC 1 cut(s) 911
NlaIII CATG 5 cut(s) 66, 482, 604, 715, 1082
NlaIV GGNNCC 2 cut(s) 505, 1011
NmuCI GTSAC 1 cut(s) 356
NsbI TGCGCA 1 cut(s) 1194
NspI RCATGY 1 cut(s) 66
OliI CACNNNNGTG 1 cut(s) 160
PctI GAATGC 1 cut(s) 1140
PfeI GAWTC 2 cut(s) 1105, 1279
PflMI CCANNNNNTGG 2 cut(s) 315, 1218
PkrI GCNGC 5 cut(s) 740, 808, 811, 975, 1032
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 676
Psp1406I AACGTT 1 cut(s) 1099
Psp5II RGGWCCY 1 cut(s) 676
Psp6I CCWGG 2 cut(s) 1144, 1199
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 2 cut(s) 1144, 1199
PspN4I GGNNCC 2 cut(s) 505, 1011
PspPI GGNCC 6 cut(s) 622, 676, 785, 932, 1085, 1221
PspPPI RGGWCCY 1 cut(s) 676
PsrI GAACNNNNNNTAC 2 cut(s) 867, 899
PstI CTGCAG 1 cut(s) 1244
PstNI CAGNNNCTG 2 cut(s) 650, 1206
PvuII CAGCTG 1 cut(s) 976
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
RseI CAYNNNNRTG 1 cut(s) 160
SaqAI TTAA 3 cut(s) 17, 408, 926
SatI GCNGC 5 cut(s) 739, 807, 810, 974, 1031
Sau3AI GATC 5 cut(s) 292, 323, 451, 1020, 1163
Sau96I GGNCC 6 cut(s) 622, 676, 785, 932, 1085, 1221
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 2 cut(s) 1146, 1201
SfaNI GCATC 3 cut(s) 673, 898, 1204
SfcI CTRYAG 1 cut(s) 1240
SinI GGWCC 5 cut(s) 622, 676, 932, 1085, 1221
SmiMI CAYNNNNRTG 1 cut(s) 160
SpeI ACTAGT 1 cut(s) 146
Sse9I AATT 5 cut(s) 269, 519, 634, 1039, 1066
SsiI CCGC 3 cut(s) 381, 588, 656
SspMI CTAG 4 cut(s) 147, 734, 912, 959
StyD4I CCNGG 2 cut(s) 1144, 1199
TaaI ACNGT 2 cut(s) 356, 942
TaiI ACGT 1 cut(s) 1102
TaqI TCGA 1 cut(s) 402
TasI AATT 5 cut(s) 269, 519, 634, 1039, 1066
TatI WGTACW 1 cut(s) 152
TfiI GAWTC 2 cut(s) 1105, 1279
Tru1I TTAA 3 cut(s) 17, 408, 926
Tru9I TTAA 3 cut(s) 17, 408, 926
TscAI CASTG 2 cut(s) 167, 832
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 5 cut(s) 738, 806, 809, 973, 1030
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 5 cut(s) 567, 617, 700, 1063, 1259
TspRI CASTG 2 cut(s) 167, 832
Van91I CCANNNNNTGG 2 cut(s) 315, 1218
VpaK11BI GGWCC 5 cut(s) 622, 676, 932, 1085, 1221
XapI RAATTY 2 cut(s) 269, 1039
XceI RCATGY 1 cut(s) 66
XmiI GTMKAC 1 cut(s) 252
XspI CTAG 4 cut(s) 147, 734, 912, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.