Prupe.1G431200_v2.0.a1

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014329
Physical Location & Seq
Forward (+)
36943080 .. 36944631
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G431200.1

Sequence Viewer

Length: 1299 bp
ATGAGAAGCTCTGCTCCCACCAGAGCTTTAGTTTCAATCTCAACCTTCTTTTTTCTACTAACCTGTCTCAGTATTGCCTACTCTGTGACAGACCATACCCACAAAGAAACCCTCAAGCTTGTTTTAGGTGGCCACCATAGGGGAGGCAGCAAAGATAGAGGCAATAGAAAGCAAATAAGCCCACCAAAAGCACCTCCAGATTTACCAAACACAGAAGAATTTGTGTTTGCAGATCAAAAGCTTGAAATAGTGTACCCAATTATCCAAAAATTCAAGTCCACCATCACCTCAGACCCTCTAGGCATCACCAAAACTTGGGTAGGCTCCAATATTTGCAGCTACAGAGGCTTCTATTGTGACAACCCCCCAAACAACGGCTCTGCAATTGCCCTGGCCTCCATTGACTTCAATGGTTTTCAACTTGGTGCTTCTTCACTTGATGGGTTTCTTGACCAACTCCCTGATATTGCCCTCTTCCATGCAAATTCCAACAATTTTTCAGGCACCATCTCTGCCAACATTGCCAAGCTCCCCTATCTCTATGAGCTTGACCTAAGTAACAACCAATTCTCTGGTCCTTTTCCCAGCGTTGTTCTTGGGATGAGCAGCCTCACTTTCTTGGACATAAGGTTCAATTTTTTCACTGGGTCAGTTCCACCTCAGATATTCACTCAGGACCTTGATGCCCTCTTCATTAACAACAACAATTTCATGCAGAAGCTTCCTGATAATTTGGGTAGCTCTCATATTCTTCTTCTCACCTTAGCAAACAACAAGTTCATGGGCCCAATTCCACCAGGGATTTCCAAAGCCTTTTCTGCTCTGACTGAAGTTCTGCTCCTAAATAACCAGTTAACAGGTTGTCTGCCTTATGAGATTGGGTTACTAAAGGAGGCCATAGTGTTTGATGCAGGCAATAACCAATTGACTGGCCCTTTGCCATTCTCTCTGGCATGCCTGGAGAATGCGGAGCAGCTCAACTTTGCTGGTAATTTGTTGTATGGCATGGTGCCAGAGGTGGTCTGTGGGCTGCAGAATTTGGTAGACTTATCATTGTCTGACAATTATTTCACAAATGTTGGACCTCTTTGTAGAATGTTGATTGGTAGAGGAGTGCTTGATGTTAGGAACAACTGCATTCCTGATCTTCCATTCCAAAAATCAATTGAAGAATGTGCAGATTTCTTTGCACACCCAAGGTTCTGTCCCTACATGGGATCTTATACTTACATTCCCTGTAAGTTTCCTCATTTTGGTTCTTCTGCTCCTCTACTTCCTCAAGTGGCTCCCCCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

433

Amino Acids

47.25

Weight (kDa)

5.61

Isoelectric Point (pI)

33.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 503, 1009
AccB7I CCANNNNNTGG 1 cut(s) 315
AccI GTMKAC 1 cut(s) 1044
AciI CCGC 1 cut(s) 968
AclWI GGATC 1 cut(s) 1225
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 4 cut(s) 218, 269, 484, 1036
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 5 cut(s) 139, 315, 374, 1214, 1253
AgsI TTSAA 7 cut(s) 36, 245, 274, 409, 419, 634, 1169
AjnI CCWGG 3 cut(s) 390, 796, 957
AloI GAACNNNNNNTCC 2 cut(s) 614, 646
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 1 cut(s) 1225
AoxI GGCC 5 cut(s) 130, 393, 784, 894, 931
ApaI GGGCCC 1 cut(s) 788
ApeKI GCWGC 5 cut(s) 147, 336, 606, 973, 1030
ApoI RAATTY 4 cut(s) 218, 269, 484, 1036
ArsI GACNNNNNNTTYG 2 cut(s) 919, 951
AspS9I GGNCC 6 cut(s) 575, 676, 784, 785, 932, 1082
AsuHPI GGTGA 3 cut(s) 277, 298, 751
AvaII GGWCC 3 cut(s) 575, 676, 1082
BaeGI GKGCMC 1 cut(s) 788
BalI TGGCCA 1 cut(s) 132
BanI GGYRCC 2 cut(s) 503, 1009
BanII GRGCYC 1 cut(s) 788
BbvI GCAGC 5 cut(s) 159, 348, 618, 985, 1017
BccI CCATC 3 cut(s) 290, 434, 515
BceAI ACGGC 1 cut(s) 391
BciT130I CCWGG 3 cut(s) 392, 798, 959
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 2 cut(s) 340, 1031
BisI GCNGC 5 cut(s) 148, 337, 607, 974, 1031
BlsI GCNGC 5 cut(s) 149, 338, 608, 975, 1032
Bme1390I CCNGG 3 cut(s) 392, 798, 959
Bme18I GGWCC 3 cut(s) 575, 676, 1082
BmgT120I GGNCC 6 cut(s) 575, 676, 784, 785, 932, 1082
BmiI GGNNCC 5 cut(s) 325, 505, 786, 1011, 1287
BmrFI CCNGG 3 cut(s) 392, 798, 959
BmrI ACTGGG 1 cut(s) 654
BmsI GCATC 3 cut(s) 312, 673, 898
BmuI ACTGGG 1 cut(s) 654
BpmI CTGGAG 2 cut(s) 180, 980
Bpu10I CCTNAGC 1 cut(s) 763
BpuEI CTTGAG 2 cut(s) 98, 1263
BsaJI CCNNGG 3 cut(s) 390, 797, 1196
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 315, 374, 1214, 1253
Bse1I ACTGG 3 cut(s) 649, 850, 934
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 3 cut(s) 392, 798, 959
BseDI CCNNGG 3 cut(s) 390, 797, 1196
BseGI GGATG 1 cut(s) 606
BseLI CCNNNNNNNGG 5 cut(s) 139, 315, 374, 1214, 1253
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 4 cut(s) 82, 303, 674, 686
BseNI ACTGG 3 cut(s) 649, 850, 934
BseRI GAGGAG 2 cut(s) 1125, 1257
BseSI GKGCMC 1 cut(s) 788
BseXI GCAGC 5 cut(s) 159, 348, 618, 985, 1017
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 1197
BshFI GGCC 5 cut(s) 132, 395, 786, 896, 933
BshNI GGYRCC 2 cut(s) 503, 1009
BslFI GGGAC 1 cut(s) 1191
BslI CCNNNNNNNGG 5 cut(s) 139, 315, 374, 1214, 1253
BsmAI GTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 1191
BsmI GAATGC 2 cut(s) 970, 1137
BsnI GGCC 5 cut(s) 132, 395, 786, 896, 933
Bsp120I GGGCCC 1 cut(s) 784
Bsp1286I GDGCHC 1 cut(s) 788
Bsp143I GATC 3 cut(s) 232, 1144, 1217
BspACI CCGC 1 cut(s) 968
BspANI GGCC 5 cut(s) 132, 395, 786, 896, 933
BspCNI CTCAG 4 cut(s) 81, 302, 673, 685
BspLI GGNNCC 5 cut(s) 325, 505, 786, 1011, 1287
BspMAI CTGCAG 1 cut(s) 1035
BspPI GGATC 1 cut(s) 1225
BspT107I GGYRCC 2 cut(s) 503, 1009
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 3 cut(s) 649, 850, 934
BssECI CCNNGG 3 cut(s) 390, 797, 1196
BssMI GATC 3 cut(s) 232, 1144, 1217
BssT1I CCWWGG 1 cut(s) 1196
Bst2UI CCWGG 3 cut(s) 392, 798, 959
Bst6I CTCTTC 2 cut(s) 479, 695
BstC8I GCNNGC 2 cut(s) 913, 955
BstDEI CTNAG 6 cut(s) 68, 289, 554, 660, 672, 763
BstF5I GGATG 1 cut(s) 606
BstKTI GATC 3 cut(s) 235, 1147, 1220
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 3 cut(s) 232, 1144, 1217
BstMWI GCNNNNNNNGC 3 cut(s) 345, 521, 818
BstNI CCWGG 3 cut(s) 392, 798, 959
BstNSI RCATGY 1 cut(s) 957
BstSCI CCNGG 3 cut(s) 390, 796, 957
BstSFI CTRYAG 2 cut(s) 340, 1031
BstSLI GKGCMC 1 cut(s) 788
BstV1I GCAGC 5 cut(s) 159, 348, 618, 985, 1017
BstX2I RGATCY 1 cut(s) 1217
BstXI CCANNNNNNTGG 2 cut(s) 572, 929
BstYI RGATCY 1 cut(s) 1217
BsuRI GGCC 5 cut(s) 132, 395, 786, 896, 933
BtsCI GGATG 1 cut(s) 606
BtsIMutI CAGTG 1 cut(s) 642
Cac8I GCNNGC 2 cut(s) 913, 955
Cfr13I GGNCC 6 cut(s) 575, 676, 784, 785, 932, 1082
Csp6I GTAC 1 cut(s) 253
CviAII CATG 6 cut(s) 479, 712, 781, 954, 1006, 1213
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 6 cut(s) 68, 289, 554, 660, 672, 763
DpnI GATC 3 cut(s) 234, 1146, 1219
DpnII GATC 3 cut(s) 232, 1144, 1217
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 2 cut(s) 479, 695
EarI CTCTTC 2 cut(s) 479, 695
Eco130I CCWWGG 1 cut(s) 1196
Eco24I GRGCYC 1 cut(s) 788
Eco47I GGWCC 3 cut(s) 575, 676, 1082
Eco57I CTGAAG 1 cut(s) 849
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 3 cut(s) 390, 796, 957
EcoT14I CCWWGG 1 cut(s) 1196
EcoT38I GRGCYC 1 cut(s) 788
ErhI CCWWGG 1 cut(s) 1196
FaeI CATG 6 cut(s) 482, 715, 784, 957, 1009, 1216
FaqI GGGAC 1 cut(s) 1191
FatI CATG 6 cut(s) 478, 711, 780, 953, 1005, 1212
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 5 cut(s) 148, 337, 607, 974, 1031
FokI GGATG 1 cut(s) 613
FriOI GRGCYC 1 cut(s) 788
Fsp4HI GCNGC 5 cut(s) 148, 337, 607, 974, 1031
FspBI CTAG 1 cut(s) 299
GluI GCNGC 5 cut(s) 148, 337, 607, 974, 1031
GsaI CCCAGC 1 cut(s) 588
GsuI CTGGAG 2 cut(s) 180, 980
HaeIII GGCC 5 cut(s) 132, 395, 786, 896, 933
Hin1II CATG 6 cut(s) 482, 715, 784, 957, 1009, 1216
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HindIII AAGCTT 3 cut(s) 116, 239, 719
HpaI GTTAAC 1 cut(s) 855
HphI GGTGA 3 cut(s) 277, 298, 751
Hpy166II GTNNAC 4 cut(s) 253, 279, 855, 1045
Hpy188I TCNGA 4 cut(s) 292, 663, 825, 1060
Hpy188III TCNNGA 5 cut(s) 197, 449, 674, 725, 1142
Hpy8I GTNNAC 4 cut(s) 253, 279, 855, 1045
HpyAV CCTTC 1 cut(s) 55
HpyF10VI GCNNNNNNNGC 3 cut(s) 345, 521, 818
HpyF3I CTNAG 6 cut(s) 68, 289, 554, 660, 672, 763
Hsp92II CATG 6 cut(s) 482, 715, 784, 957, 1009, 1216
KspAI GTTAAC 1 cut(s) 855
Kzo9I GATC 3 cut(s) 232, 1144, 1217
LmnI GCTCC 7 cut(s) 19, 329, 534, 843, 970, 1270, 1291
Lsp1109I GCAGC 5 cut(s) 159, 348, 618, 985, 1017
LweI GCATC 3 cut(s) 312, 673, 898
MaeI CTAG 1 cut(s) 299
MaeIII GTNAC 4 cut(s) 85, 356, 557, 882
MalI GATC 3 cut(s) 234, 1146, 1219
MboI GATC 3 cut(s) 232, 1144, 1217
MboII GAAGA 9 cut(s) 227, 423, 466, 682, 743, 746, 1139, 1181, 1251
MfeI CAATTG 3 cut(s) 384, 923, 1164
MflI RGATCY 1 cut(s) 1217
MhlI GDGCHC 1 cut(s) 788
MlsI TGGCCA 1 cut(s) 132
MluNI TGGCCA 1 cut(s) 132
MmeI TCCRAC 2 cut(s) 513, 1060
Mox20I TGGCCA 1 cut(s) 132
MscI TGGCCA 1 cut(s) 132
MseI TTAA 2 cut(s) 696, 854
Msp20I TGGCCA 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 392, 798, 959
MunI CAATTG 3 cut(s) 384, 923, 1164
Mva1269I GAATGC 2 cut(s) 970, 1137
MvaI CCWGG 3 cut(s) 392, 798, 959
MwoI GCNNNNNNNGC 3 cut(s) 345, 521, 818
NdeII GATC 3 cut(s) 232, 1144, 1217
NlaIII CATG 6 cut(s) 482, 715, 784, 957, 1009, 1216
NlaIV GGNNCC 5 cut(s) 325, 505, 786, 1011, 1287
NmuCI GTSAC 2 cut(s) 85, 356
NspI RCATGY 1 cut(s) 957
PaeI GCATGC 1 cut(s) 957
PctI GAATGC 2 cut(s) 970, 1137
PflMI CCANNNNNTGG 1 cut(s) 315
PkrI GCNGC 5 cut(s) 149, 338, 608, 975, 1032
PpuMI RGGWCCY 1 cut(s) 676
Psp5II RGGWCCY 1 cut(s) 676
Psp6I CCWGG 3 cut(s) 390, 796, 957
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 3 cut(s) 390, 796, 957
PspN4I GGNNCC 5 cut(s) 325, 505, 786, 1011, 1287
PspOMI GGGCCC 1 cut(s) 784
PspPI GGNCC 6 cut(s) 575, 676, 784, 785, 932, 1082
PspPPI RGGWCCY 1 cut(s) 676
PstI CTGCAG 1 cut(s) 1035
PsuI RGATCY 1 cut(s) 1217
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SaqAI TTAA 2 cut(s) 696, 854
SatI GCNGC 5 cut(s) 148, 337, 607, 974, 1031
Sau3AI GATC 3 cut(s) 232, 1144, 1217
Sau96I GGNCC 6 cut(s) 575, 676, 784, 785, 932, 1082
ScrFI CCNGG 3 cut(s) 392, 798, 959
SduI GDGCHC 1 cut(s) 788
SfaNI GCATC 3 cut(s) 312, 673, 898
SfcI CTRYAG 2 cut(s) 340, 1031
SinI GGWCC 3 cut(s) 575, 676, 1082
SmlI CTYRAG 2 cut(s) 113, 1278
SmoI CTYRAG 2 cut(s) 113, 1278
SphI GCATGC 1 cut(s) 957
SsiI CCGC 1 cut(s) 968
SspI AATATT 1 cut(s) 331
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 3 cut(s) 390, 796, 957
StyI CCWWGG 1 cut(s) 1196
Tru1I TTAA 2 cut(s) 696, 854
Tru9I TTAA 2 cut(s) 696, 854
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 2 cut(s) 85, 356
TseI GCWGC 5 cut(s) 147, 336, 606, 973, 1030
Tsp45I GTSAC 2 cut(s) 85, 356
TspDTI ATGAA 3 cut(s) 682, 700, 769
TspRI CASTG 1 cut(s) 649
Van91I CCANNNNNTGG 1 cut(s) 315
VpaK11BI GGWCC 3 cut(s) 575, 676, 1082
XapI RAATTY 4 cut(s) 218, 269, 484, 1036
XceI RCATGY 1 cut(s) 957
XmiI GTMKAC 1 cut(s) 1044
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.