MD15G1078100.v1.1

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014329
Physical Location & Seq
Reverse (-)
5330743 .. 5332131
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1078100.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGAGAAGCTCTGATTCCACCAAAGCTCTAGTTTCATTCTCAACCTTCTTTTTTCTTCTATCCTATCTTTGTATTCCTAGTTGTGTAGCAGTAGCATTCTCGTTGCATGAAAATTCTTCTCCCGTAACAAACTATTCCAACAGACAAACCCTCGAGTTCGATTTAGGTGGCCACCGCAGAAGAGGCAGCGGACACAGAAGCCACCAAAAGCACAAAACCCCACCAGCAGCACCACCACCACCACCGGTACCACCACCACAAGTACCACTACGGCCGCCACAAGCACCTCCTCCTACAAAATTGCCAGACAACCAACTTGTATTTGCAGACCAAAGGCTCAAAATAGTCTACCCAATTATCCAAAACTTCAAGTCCCTTATCACCTCAGATCCACTAAACATCACAAAAACGTGGGTGGGATCCAACATATGCAGTTACAAAGGCTTCTACTGTGACAGCCCTCCAAACAACAGCTCCGCCATTGCCCTCGCCTCGATCGACTTCAACGGATTCCAACTAGGGGCCCCTTCTCTCGACGGATTTCTCGACCAGCTCCCGGACATTGCCCTCTTCCATGCAAACTCCAACAATTTTTCCGGCACTGTCTCCCCCGCCATTGCCAACCTCCCCTACCTCTACGAACTCGACATAAGCAACAACCAATTCTCCGGGGCGTTTCCCTCCGCCGTTCTCGGCATGAACACCCTAGGTTTCTTGGACATAAGGTTCAATTTCTTCACTGGGGCAGTCCCACCGGAGATTTTCACTCAAGAACTTGACGCCCTATTCCTAAACAACAACAATTTCATGCAGAATCTTCCCGACAATCTCGGCAGCTCTCATATTCTCCTCCTCACCTTAGCAAACAACAAATTCACGGGCTCGATTCCATCGGGCATTTCAAAAGCTTTCGCTGCTCTGACTGAGGTTCTGCTACTGAACAACCAGCTCACAGGATGCTTGCCTTACGAAATAGGGTTGCTAAAACAGGCCTTAGTTTTAGATGTCGGAAACAACCAGTTAACCGGGCCGTTACCGTTTTCACTGGCGTGCCTCGAAAACGTGGAGCAGCTGAATTTTGCGGGTAACTTGTTGCATGGCATGGTACCTGAGGTGGTGTGTCATGAGCTGCAAAGCTTGGAGAACTTTTCGCTGTCCGATAATTACTTCACAAATGTTGGTCCGATTTGTAGAAGCTTGATCGGGAAAGGAGTGCTCGATGTTACGAACAACTGCGTTCCCGATCTTCCGTTTCAGAGACCGGTGGAGGAATGTGCGGATTTCTTTGCGCACCCAAGGTTTTGTCCCTTCATGTGGACTTATACTTACATTCCATGTAAGCCTCCTGGTTTTGGTTCTTTGTTTCCTCCATTGCCTCCTCCACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

463

Amino Acids

50.52

Weight (kDa)

5.66

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1290
Acc65I GGTACC 2 cut(s) 247, 1105
AccB1I GGYRCC 2 cut(s) 247, 1105
AccI GTMKAC 1 cut(s) 348
AciI CCGC 8 cut(s) 175, 189, 275, 477, 612, 684, 1082, 1277
AclWI GGATC 3 cut(s) 383, 414, 427
AcoI YGGCCR 2 cut(s) 169, 272
AcsI RAATTY 3 cut(s) 112, 872, 1075
AcyI GRCGYC 1 cut(s) 780
AfaI GTAC 3 cut(s) 249, 264, 1107
AfiI CCNNNNNNNGG 4 cut(s) 520, 556, 1314, 1352
AgeI ACCGGT 2 cut(s) 244, 1261
AgsI TTSAA 4 cut(s) 370, 505, 730, 903
AjnI CCWGG 1 cut(s) 1345
AleI CACNNNNGTG 1 cut(s) 1048
AloI GAACNNNNNNTCC 2 cut(s) 710, 742
Alw21I GWGCWC 1 cut(s) 1218
Alw26I GTCTC 2 cut(s) 610, 1252
AlwI GGATC 3 cut(s) 383, 414, 427
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 5 cut(s) 169, 272, 522, 990, 1028
ApaI GGGCCC 1 cut(s) 526
ApeKI GCWGC 6 cut(s) 186, 227, 834, 914, 1069, 1129
ApoI RAATTY 3 cut(s) 112, 872, 1075
AsiGI ACCGGT 2 cut(s) 244, 1261
Asp718I GGTACC 2 cut(s) 247, 1105
AspA2I CCTAGG 1 cut(s) 706
AspLEI GCGC 1 cut(s) 1291
AspS9I GGNCC 4 cut(s) 522, 523, 1028, 1181
AsuC2I CCSGG 3 cut(s) 557, 670, 1027
AsuHPI GGTGA 2 cut(s) 373, 847
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 1181
AvrII CCTAGG 1 cut(s) 706
AxyI CCTNAGG 1 cut(s) 1110
BaeGI GKGCMC 1 cut(s) 526
BalI TGGCCA 1 cut(s) 171
BamHI GGATCC 1 cut(s) 419
BanI GGYRCC 2 cut(s) 247, 1105
BanII GRGCYC 2 cut(s) 526, 884
Bbv12I GWGCWC 1 cut(s) 1218
BbvI GCAGC 6 cut(s) 198, 239, 846, 901, 1081, 1116
BccI CCATC 1 cut(s) 898
BceAI ACGGC 3 cut(s) 287, 671, 1015
BciT130I CCWGG 1 cut(s) 1347
BcnI CCSGG 3 cut(s) 557, 670, 1027
BcoDI GTCTC 2 cut(s) 610, 1252
BfaI CTAG 4 cut(s) 29, 78, 518, 707
BisI GCNGC 7 cut(s) 187, 228, 275, 835, 915, 1070, 1130
BlnI CCTAGG 1 cut(s) 706
BlsI GCNGC 7 cut(s) 188, 229, 276, 836, 916, 1071, 1131
Bme1390I CCNGG 4 cut(s) 557, 670, 1027, 1347
Bme18I GGWCC 1 cut(s) 1181
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 4 cut(s) 522, 523, 1028, 1181
BmiI GGNNCC 6 cut(s) 249, 421, 523, 524, 525, 1107
BmrFI CCNGG 4 cut(s) 557, 670, 1027, 1347
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 1 cut(s) 947
BmuI ACTGGG 1 cut(s) 750
Bpu10I CCTNAGC 1 cut(s) 859
BpuEI CTTGAG 1 cut(s) 753
BpuMI CCSGG 3 cut(s) 557, 670, 1027
BsaHI GRCGYC 1 cut(s) 780
BsaI GGTCTC 1 cut(s) 1252
BsaJI CCNNGG 3 cut(s) 669, 706, 1295
BsaWI WCCGGW 3 cut(s) 244, 754, 1261
BsaXI ACNNNNNCTCC 4 cut(s) 458, 488, 650, 680
Bsc4I CCNNNNNNNGG 4 cut(s) 520, 556, 1314, 1352
Bse118I RCCGGY 2 cut(s) 244, 1261
Bse1I ACTGG 3 cut(s) 745, 1018, 1050
Bse21I CCTNAGG 1 cut(s) 1110
Bse3DI GCAATG 4 cut(s) 480, 561, 615, 1370
BseBI CCWGG 1 cut(s) 1347
BseDI CCNNGG 3 cut(s) 669, 706, 1295
BseGI GGATG 1 cut(s) 962
BseLI CCNNNNNNNGG 4 cut(s) 520, 556, 1314, 1352
BseMI GCAATG 4 cut(s) 480, 561, 615, 1370
BseMII CTCAG 3 cut(s) 399, 915, 1101
BseNI ACTGG 3 cut(s) 745, 1018, 1050
BseRI GAGGAG 4 cut(s) 279, 839, 842, 1368
BseSI GKGCMC 1 cut(s) 526
BseX3I CGGCCG 1 cut(s) 272
BseXI GCAGC 6 cut(s) 198, 239, 846, 901, 1081, 1116
Bsh1285I CGRYCG 2 cut(s) 275, 498
BshFI GGCC 5 cut(s) 171, 274, 524, 992, 1030
BshNI GGYRCC 2 cut(s) 247, 1105
BshTI ACCGGT 2 cut(s) 244, 1261
BsiEI CGRYCG 2 cut(s) 275, 498
BsiHKAI GWGCWC 1 cut(s) 1218
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 7 cut(s) 245, 557, 597, 669, 755, 1026, 1262
BslFI GGGAC 3 cut(s) 358, 734, 1290
BslI CCNNNNNNNGG 4 cut(s) 520, 556, 1314, 1352
BsmAI GTCTC 2 cut(s) 610, 1252
BsmFI GGGAC 3 cut(s) 358, 734, 1290
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 5 cut(s) 171, 274, 524, 992, 1030
Bso31I GGTCTC 1 cut(s) 1252
BsoBI CYCGRG 1 cut(s) 152
Bsp120I GGGCCC 1 cut(s) 522
Bsp1286I GDGCHC 3 cut(s) 526, 884, 1218
Bsp143I GATC 5 cut(s) 388, 419, 495, 1200, 1243
BspACI CCGC 8 cut(s) 175, 189, 275, 477, 612, 684, 1082, 1277
BspANI GGCC 5 cut(s) 171, 274, 524, 992, 1030
BspCNI CTCAG 3 cut(s) 398, 916, 1102
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 6 cut(s) 249, 421, 523, 524, 525, 1107
BspPI GGATC 3 cut(s) 383, 414, 427
BspT107I GGYRCC 2 cut(s) 247, 1105
BspTNI GGTCTC 1 cut(s) 1252
BsrDI GCAATG 4 cut(s) 480, 561, 615, 1370
BsrFI RCCGGY 2 cut(s) 244, 1261
BsrI ACTGG 3 cut(s) 745, 1018, 1050
BssAI RCCGGY 2 cut(s) 244, 1261
BssECI CCNNGG 3 cut(s) 669, 706, 1295
BssMI GATC 5 cut(s) 388, 419, 495, 1200, 1243
BssNI GRCGYC 1 cut(s) 780
BssT1I CCWWGG 2 cut(s) 706, 1295
Bst2UI CCWGG 1 cut(s) 1347
Bst4CI ACNGT 4 cut(s) 452, 604, 1038, 1386
Bst6I CTCTTC 2 cut(s) 175, 575
BstACI GRCGYC 1 cut(s) 780
BstC8I GCNNGC 2 cut(s) 962, 1051
BstDEI CTNAG 5 cut(s) 385, 859, 924, 994, 1110
BstF5I GGATG 1 cut(s) 962
BstHHI GCGC 1 cut(s) 1291
BstKTI GATC 5 cut(s) 391, 422, 498, 1203, 1246
BstMAI GTCTC 2 cut(s) 610, 1252
BstMBI GATC 5 cut(s) 388, 419, 495, 1200, 1243
BstMCI CGRYCG 2 cut(s) 275, 498
BstMWI GCNNNNNNNGC 2 cut(s) 183, 914
BstNI CCWGG 1 cut(s) 1347
BstSCI CCNGG 4 cut(s) 555, 668, 1025, 1345
BstSLI GKGCMC 1 cut(s) 526
BstV1I GCAGC 6 cut(s) 198, 239, 846, 901, 1081, 1116
BstX2I RGATCY 2 cut(s) 388, 419
BstYI RGATCY 2 cut(s) 388, 419
BstZI CGGCCG 1 cut(s) 272
Bsu36I CCTNAGG 1 cut(s) 1110
BsuRI GGCC 5 cut(s) 171, 274, 524, 992, 1030
BtsCI GGATG 1 cut(s) 962
BtsIMutI CAGTG 3 cut(s) 600, 738, 1043
Cac8I GCNNGC 2 cut(s) 962, 1051
CciI TCATGA 1 cut(s) 1123
CfoI GCGC 1 cut(s) 1291
Cfr10I RCCGGY 2 cut(s) 244, 1261
Cfr13I GGNCC 4 cut(s) 522, 523, 1028, 1181
CseI GACGC 1 cut(s) 788
Csp6I GTAC 3 cut(s) 248, 263, 1106
CspAI ACCGGT 2 cut(s) 244, 1261
CviAII CATG 9 cut(s) 107, 575, 697, 808, 1097, 1102, 1124, 1312, 1335
CviQI GTAC 3 cut(s) 248, 263, 1106
DdeI CTNAG 5 cut(s) 385, 859, 924, 994, 1110
DpnI GATC 5 cut(s) 390, 421, 497, 1202, 1245
DpnII GATC 5 cut(s) 388, 419, 495, 1200, 1243
EaeI YGGCCR 2 cut(s) 169, 272
EagI CGGCCG 1 cut(s) 272
Eam1104I CTCTTC 2 cut(s) 175, 575
EarI CTCTTC 2 cut(s) 175, 575
EciI GGCGGA 2 cut(s) 466, 673
EclXI CGGCCG 1 cut(s) 272
Eco130I CCWWGG 2 cut(s) 706, 1295
Eco147I AGGCCT 1 cut(s) 992
Eco24I GRGCYC 2 cut(s) 526, 884
Eco31I GGTCTC 1 cut(s) 1252
Eco47I GGWCC 1 cut(s) 1181
Eco52I CGGCCG 1 cut(s) 272
Eco81I CCTNAGG 1 cut(s) 1110
Eco88I CYCGRG 1 cut(s) 152
EcoO109I RGGNCCY 2 cut(s) 522, 523
EcoRII CCWGG 1 cut(s) 1345
EcoT14I CCWWGG 2 cut(s) 706, 1295
EcoT38I GRGCYC 2 cut(s) 526, 884
ErhI CCWWGG 2 cut(s) 706, 1295
FaeI CATG 9 cut(s) 110, 578, 700, 811, 1100, 1105, 1127, 1315, 1338
FaqI GGGAC 3 cut(s) 358, 734, 1290
FatI CATG 9 cut(s) 106, 574, 696, 807, 1096, 1101, 1123, 1311, 1334
FauI CCCGC 2 cut(s) 619, 1075
FauNDI CATATG 1 cut(s) 428
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 7 cut(s) 187, 228, 275, 835, 915, 1070, 1130
FokI GGATG 1 cut(s) 969
FriOI GRGCYC 2 cut(s) 526, 884
Fsp4HI GCNGC 7 cut(s) 187, 228, 275, 835, 915, 1070, 1130
FspBI CTAG 4 cut(s) 29, 78, 518, 707
FspI TGCGCA 1 cut(s) 1290
GlaI GCGC 1 cut(s) 1290
GluI GCNGC 7 cut(s) 187, 228, 275, 835, 915, 1070, 1130
HaeIII GGCC 5 cut(s) 171, 274, 524, 992, 1030
HapII CCGG 7 cut(s) 245, 557, 597, 669, 755, 1026, 1262
HgaI GACGC 1 cut(s) 788
HhaI GCGC 1 cut(s) 1291
Hin1I GRCGYC 1 cut(s) 780
Hin1II CATG 9 cut(s) 110, 578, 700, 811, 1100, 1105, 1127, 1315, 1338
Hin6I GCGC 1 cut(s) 1289
HinP1I GCGC 1 cut(s) 1289
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HindIII AAGCTT 3 cut(s) 906, 1135, 1195
HinfI GANTC 4 cut(s) 14, 510, 814, 886
HpaI GTTAAC 1 cut(s) 1023
HpaII CCGG 7 cut(s) 245, 557, 597, 669, 755, 1026, 1262
HphI GGTGA 2 cut(s) 373, 847
Hpy166II GTNNAC 3 cut(s) 349, 1023, 1317
Hpy188I TCNGA 7 cut(s) 13, 388, 921, 1010, 1159, 1185, 1257
Hpy188III TCNNGA 7 cut(s) 533, 545, 770, 821, 1124, 1204, 1241
Hpy8I GTNNAC 3 cut(s) 349, 1023, 1317
Hpy99I CGWCG 1 cut(s) 539
HpyAV CCTTC 3 cut(s) 55, 537, 1318
HpyCH4III ACNGT 4 cut(s) 452, 604, 1038, 1386
HpyCH4IV ACGT 2 cut(s) 410, 1062
HpyCH4V TGCA 7 cut(s) 106, 326, 432, 578, 811, 1096, 1132
HpyF10VI GCNNNNNNNGC 2 cut(s) 183, 914
HpyF3I CTNAG 5 cut(s) 385, 859, 924, 994, 1110
HpySE526I ACGT 2 cut(s) 410, 1062
Hsp92I GRCGYC 1 cut(s) 780
Hsp92II CATG 9 cut(s) 110, 578, 700, 811, 1100, 1105, 1127, 1315, 1338
HspAI GCGC 1 cut(s) 1289
KpnI GGTACC 2 cut(s) 251, 1109
KspAI GTTAAC 1 cut(s) 1023
Kzo9I GATC 5 cut(s) 388, 419, 495, 1200, 1243
LmnI GCTCC 3 cut(s) 479, 558, 1066
Lsp1109I GCAGC 6 cut(s) 198, 239, 846, 901, 1081, 1116
LweI GCATC 1 cut(s) 947
MaeI CTAG 4 cut(s) 29, 78, 518, 707
MaeII ACGT 2 cut(s) 410, 1062
MaeIII GTNAC 6 cut(s) 124, 434, 452, 1032, 1085, 1222
MalI GATC 5 cut(s) 390, 421, 497, 1202, 1245
MboI GATC 5 cut(s) 388, 419, 495, 1200, 1243
MboII GAAGA 7 cut(s) 47, 108, 192, 562, 727, 809, 1238
MflI RGATCY 2 cut(s) 388, 419
MhlI GDGCHC 3 cut(s) 526, 884, 1218
MlsI TGGCCA 1 cut(s) 171
MluNI TGGCCA 1 cut(s) 171
MmeI TCCRAC 5 cut(s) 162, 447, 538, 609, 988
Mox20I TGGCCA 1 cut(s) 171
MscI TGGCCA 1 cut(s) 171
MseI TTAA 1 cut(s) 1022
MslI CAYNNNNRTG 1 cut(s) 1048
Msp20I TGGCCA 1 cut(s) 171
MspA1I CMGCKG 2 cut(s) 189, 1072
MspI CCGG 7 cut(s) 245, 557, 597, 669, 755, 1026, 1262
MspR9I CCNGG 4 cut(s) 557, 670, 1027, 1347
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 1 cut(s) 1347
MwoI GCNNNNNNNGC 2 cut(s) 183, 914
NciI CCSGG 3 cut(s) 557, 670, 1027
NdeI CATATG 1 cut(s) 428
NdeII GATC 5 cut(s) 388, 419, 495, 1200, 1243
NlaIII CATG 9 cut(s) 110, 578, 700, 811, 1100, 1105, 1127, 1315, 1338
NlaIV GGNNCC 6 cut(s) 249, 421, 523, 524, 525, 1107
NmeAIII GCCGAG 2 cut(s) 672, 810
NmuCI GTSAC 1 cut(s) 452
NsbI TGCGCA 1 cut(s) 1290
OliI CACNNNNGTG 1 cut(s) 1048
PaeR7I CTCGAG 1 cut(s) 152
PagI TCATGA 1 cut(s) 1123
PceI AGGCCT 1 cut(s) 992
PcsI WCGNNNNNNNCGW 2 cut(s) 495, 543
PctI GAATGC 1 cut(s) 95
PfeI GAWTC 4 cut(s) 14, 510, 814, 886
PfoI TCCNGGA 1 cut(s) 555
PinAI ACCGGT 2 cut(s) 244, 1261
PkrI GCNGC 7 cut(s) 188, 229, 276, 836, 916, 1071, 1131
Ple19I CGATCG 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 1345
PspGI CCWGG 1 cut(s) 1345
PspN4I GGNNCC 6 cut(s) 249, 421, 523, 524, 525, 1107
PspOMI GGGCCC 1 cut(s) 522
PspPI GGNCC 4 cut(s) 522, 523, 1028, 1181
PspXI VCTCGAGB 1 cut(s) 152
PsuI RGATCY 2 cut(s) 388, 419
PvuI CGATCG 1 cut(s) 498
PvuII CAGCTG 1 cut(s) 1072
RsaI GTAC 3 cut(s) 249, 264, 1107
RsaNI GTAC 3 cut(s) 248, 263, 1106
RseI CAYNNNNRTG 1 cut(s) 1048
SaqAI TTAA 1 cut(s) 1022
SatI GCNGC 7 cut(s) 187, 228, 275, 835, 915, 1070, 1130
Sau3AI GATC 5 cut(s) 388, 419, 495, 1200, 1243
Sau96I GGNCC 4 cut(s) 522, 523, 1028, 1181
ScrFI CCNGG 4 cut(s) 557, 670, 1027, 1347
SduI GDGCHC 3 cut(s) 526, 884, 1218
SfaNI GCATC 1 cut(s) 947
Sfr274I CTCGAG 1 cut(s) 152
SinI GGWCC 1 cut(s) 1181
SlaI CTCGAG 1 cut(s) 152
SmiMI CAYNNNNRTG 1 cut(s) 1048
SmlI CTYRAG 2 cut(s) 152, 768
SmoI CTYRAG 2 cut(s) 152, 768
SseBI AGGCCT 1 cut(s) 992
SsiI CCGC 8 cut(s) 175, 189, 275, 477, 612, 684, 1082, 1277
SspMI CTAG 4 cut(s) 29, 78, 518, 707
StuI AGGCCT 1 cut(s) 992
StyD4I CCNGG 4 cut(s) 555, 668, 1025, 1345
StyI CCWWGG 2 cut(s) 706, 1295
TaaI ACNGT 4 cut(s) 452, 604, 1038, 1386
TaiI ACGT 2 cut(s) 413, 1065
TauI GCSGC 1 cut(s) 277
TfiI GAWTC 4 cut(s) 14, 510, 814, 886
Tru1I TTAA 1 cut(s) 1022
Tru9I TTAA 1 cut(s) 1022
TscAI CASTG 3 cut(s) 607, 745, 1050
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 6 cut(s) 186, 227, 834, 914, 1069, 1129
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 5 cut(s) 24, 123, 713, 796, 1300
TspGWI ACGGA 3 cut(s) 522, 552, 1239
TspRI CASTG 3 cut(s) 607, 745, 1050
VpaK11BI GGWCC 1 cut(s) 1181
XapI RAATTY 3 cut(s) 112, 872, 1075
XhoI CTCGAG 1 cut(s) 152
XmaJI CCTAGG 1 cut(s) 706
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 4 cut(s) 29, 78, 518, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.