Rroxscaffold_7G00160090

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014329
Physical Location & Seq
Forward (+)
3114936 .. 3116219
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160090.1

Sequence Viewer

Length: 1284 bp
ATGAGAAGAAGCGCTTCAACCAAAGCTTTGTTTTTAACCTTCTTCTTCTTCCTCTTAACATGCCTCATTGCAAACTCTGCAACTCACAACATTACTAGAAGAAGTAGCAAAGACAAAACCCACAGAAAGAAATGTCCTATAACCAGTAGTCTACTCCAGTGTTCGAAAACACCACCGCCACCACCAGATGTACCAAACCCACAAAAAGTGCTCTCATTTGCAGACCAAAGGCTTCAAGTGGTATACCCAGTCATTCAAAAATTCAAGTCCATCATCACCTCAGATCCTCTCAACATCACCAAAACTTGGGTAGGATCAGATATATGCAAATACAAAGGCTTCTACTGTGACAATCCACCATACAACTTATCCGCAATCGCGCTAGCCTCCATCGACTTCAATGGCTTCCAACTCGGTGCTTCCTCCCTTGATGGGTTTCTTGATCAGCTCCCTGATATTGCTCTGTTCCATGCAAACTCCAACAACTTTTCCGGCACCATTTCCTCCAAAATTGCCAACCTCTCCTACCTCTATGAGCTTGACATAAGCAACAACTTATTCTCCGGTCCATTCCCAACCGCAGTTCTCGGCATGAACGGCTTAACTTTCTTGGACATAAGGTTCAATTTCTTCACCGGCTCTGTTCCGCCTCAGATTTTCACTCAGGACCTTGATGCTCTCTTCCTCAACAACAACAACTTCATGCAGAGCCTTCCTGATAACCTAGGCAGCTCCCATATAATCCTCCTCACTTTGGCAAACAACAACTTCAATGGCCCTATTCCAAGCAGCATTGCAAAAGCTCTGTCTTCTCTCACTGAAGTTCTGCTTCTCAATAACCAGTTAACAGGTTGTTTGCCTTATGAGCTAGGTTTTTTAAAGGAGGCCATAGTGTTTGATGCTAGCAACAATCAGTTAACTGGTCCGTTACCGTTCTCTTTGGCTTGTCTAGACAAAGTGGAGCAGCTAAGTTTTGCTGGAAACTTATTGTTTGGCTTGGTGCCGGAGGTGATCTGTGATCAGCTGCTGAATTTGGCAAACTTTTCGCTGTCCAATAATTACTTCATCCATGTTGGTCCAATATGTCAAAGTTTGATCCAGAGAGGACTGCTTGATGTCACTAACAACTGCATTCCTGGTCTTCCATTCCAAAGATCGGTAGAAGAATGTGCAGATTTCTTCGCGCATCCCATGTTCTGTCCGAATATGGGGTCTTATACCTGTATTCCCTGCAGTAAGTTTCCTTATACTTCTTTTGTTCATCAAGAGGATCCTACACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

47.07

Weight (kDa)

5.96

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G06744
fragaria_vesca FvH4_2g33930
malus_domestica MD15G1078100.v1.1
prunus_persica Prupe.1G431200_v2.0.a1
pyrus_communis pycom08g07700 pycom15g07290
rosa_chinensis RchiOBHm_Chr6g0308481
rosa_laevigata RLG00000010639
rosa_multiflora Rmu_sc0001485.1_g000008 Rmu_sc0011796.1_g000011
rosa_roxburghii Rroxscaffold_7G00160090
rosa_rugosa Rorug06G0367100
rosa_samantha Rh6AG476900 Rh6BG487100 Rh6CG492200 Rh6DG478300
rosa_wichuraiana Rw6G041600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 494, 1000
AccB7I CCANNNNNTGG 1 cut(s) 306
AccI GTMKAC 2 cut(s) 151, 243
AccII CGCG 2 cut(s) 380, 1184
AciI CCGC 4 cut(s) 176, 372, 579, 647
AclWI GGATC 5 cut(s) 278, 322, 1090, 1265, 1278
AcsI RAATTY 2 cut(s) 260, 1030
AcuI CTGAAG 1 cut(s) 840
AfaI GTAC 1 cut(s) 192
AfeI AGCGCT 1 cut(s) 13
AfiI CCNNNNNNNGG 5 cut(s) 306, 432, 754, 1156, 1208
AgsI TTSAA 7 cut(s) 18, 236, 257, 265, 400, 625, 772
AjnI CCWGG 1 cut(s) 1135
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AloI GAACNNNNNNTCC 2 cut(s) 605, 637
AluBI AGCT 8 cut(s) 26, 448, 538, 732, 803, 868, 967, 1024
AluI AGCT 8 cut(s) 26, 448, 538, 732, 803, 868, 967, 1024
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 5 cut(s) 278, 322, 1090, 1265, 1278
AlwNI CAGNNNCTG 1 cut(s) 1027
Aor51HI AGCGCT 1 cut(s) 13
AoxI GGCC 2 cut(s) 775, 885
ApeKI GCWGC 4 cut(s) 729, 789, 964, 1024
ApoI RAATTY 2 cut(s) 260, 1030
ArsI GACNNNNNNTTYG 4 cut(s) 791, 823, 1196, 1228
Asp700I GAANNNNTTC 1 cut(s) 13
AspA2I CCTAGG 1 cut(s) 724
AspLEI GCGC 3 cut(s) 14, 382, 1186
AspS9I GGNCC 5 cut(s) 566, 667, 776, 923, 1076
AsuHPI GGTGA 4 cut(s) 268, 289, 625, 1021
AsuII TTCGAA 1 cut(s) 164
AsuNHI GCTAGC 2 cut(s) 382, 902
AvaII GGWCC 4 cut(s) 566, 667, 923, 1076
AvrII CCTAGG 1 cut(s) 724
BamHI GGATCC 1 cut(s) 1270
BanI GGYRCC 2 cut(s) 494, 1000
BbsI GAAGAC 2 cut(s) 801, 1133
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 741, 801, 976, 1011
BccI CCATC 3 cut(s) 278, 398, 425
BceAI ACGGC 1 cut(s) 613
BcgI CGANNNNNNTGC 2 cut(s) 1026, 1060
BciT130I CCWGG 1 cut(s) 1137
BclI TGATCA 2 cut(s) 442, 1018
BfaI CTAG 6 cut(s) 96, 383, 725, 869, 903, 950
BfmI CTRYAG 1 cut(s) 1231
BfoI RGCGCY 1 cut(s) 15
BisI GCNGC 4 cut(s) 730, 790, 965, 1025
BlnI CCTAGG 1 cut(s) 724
BlsI GCNGC 4 cut(s) 731, 791, 966, 1026
Bme1390I CCNGG 1 cut(s) 1137
Bme18I GGWCC 4 cut(s) 566, 667, 923, 1076
BmgT120I GGNCC 5 cut(s) 566, 667, 776, 923, 1076
BmiI GGNNCC 3 cut(s) 496, 1002, 1272
BmrFI CCNGG 1 cut(s) 1137
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 3 cut(s) 664, 889, 1195
BmtI GCTAGC 2 cut(s) 386, 906
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 2 cut(s) 801, 1133
BpmI CTGGAG 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 724
BsaWI WCCGGW 1 cut(s) 563
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 5 cut(s) 306, 432, 754, 1156, 1208
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 5 cut(s) 144, 157, 248, 841, 925
Bse3DI GCAATG 2 cut(s) 66, 792
BseBI CCWGG 1 cut(s) 1137
BseDI CCNNGG 1 cut(s) 724
BseGI GGATG 2 cut(s) 1065, 1186
BseLI CCNNNNNNNGG 5 cut(s) 306, 432, 754, 1156, 1208
BseMI GCAATG 2 cut(s) 66, 792
BseMII CTCAG 3 cut(s) 294, 665, 677
BseNI ACTGG 5 cut(s) 144, 157, 248, 841, 925
BseRI GAGGAG 1 cut(s) 737
BseXI GCAGC 4 cut(s) 741, 801, 976, 1011
BsgI GTGCAG 1 cut(s) 1191
Bsh1236I CGCG 2 cut(s) 380, 1184
BshFI GGCC 2 cut(s) 777, 887
BshNI GGYRCC 2 cut(s) 494, 1000
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 4 cut(s) 492, 564, 636, 1004
BslI CCNNNNNNNGG 5 cut(s) 306, 432, 754, 1156, 1208
BsmI GAATGC 1 cut(s) 1131
BsnI GGCC 2 cut(s) 777, 887
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 8 cut(s) 283, 314, 442, 1011, 1018, 1095, 1154, 1270
BspACI CCGC 4 cut(s) 176, 372, 579, 647
BspANI GGCC 2 cut(s) 777, 887
BspCNI CTCAG 3 cut(s) 293, 664, 676
BspFNI CGCG 2 cut(s) 380, 1184
BspLI GGNNCC 3 cut(s) 496, 1002, 1272
BspMAI CTGCAG 1 cut(s) 1235
BspOI GCTAGC 2 cut(s) 386, 906
BspPI GGATC 5 cut(s) 278, 322, 1090, 1265, 1278
BspT104I TTCGAA 1 cut(s) 164
BspT107I GGYRCC 2 cut(s) 494, 1000
BsrDI GCAATG 2 cut(s) 66, 792
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 5 cut(s) 144, 157, 248, 841, 925
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 1 cut(s) 724
BssMI GATC 8 cut(s) 283, 314, 442, 1011, 1018, 1095, 1154, 1270
BssNAI GTATAC 1 cut(s) 244
BssT1I CCWWGG 1 cut(s) 724
Bst1107I GTATAC 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 1137
Bst4CI ACNGT 2 cut(s) 347, 933
Bst6I CTCTTC 1 cut(s) 686
BstAPI GCANNNNNTGC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 384, 904
BstDEI CTNAG 4 cut(s) 280, 651, 663, 968
BstF5I GGATG 2 cut(s) 1065, 1186
BstFNI CGCG 2 cut(s) 380, 1184
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 3 cut(s) 14, 382, 1186
BstKTI GATC 8 cut(s) 286, 317, 445, 1014, 1021, 1098, 1157, 1273
BstMBI GATC 8 cut(s) 283, 314, 442, 1011, 1018, 1095, 1154, 1270
BstMWI GCNNNNNNNGC 3 cut(s) 77, 597, 865
BstNI CCWGG 1 cut(s) 1137
BstNSI RCATGY 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 1135
BstSFI CTRYAG 1 cut(s) 1231
BstUI CGCG 2 cut(s) 380, 1184
BstV1I GCAGC 4 cut(s) 741, 801, 976, 1011
BstV2I GAAGAC 2 cut(s) 801, 1133
BstX2I RGATCY 2 cut(s) 283, 1270
BstYI RGATCY 2 cut(s) 283, 1270
BstZ17I GTATAC 1 cut(s) 244
BsuRI GGCC 2 cut(s) 777, 887
BtsCI GGATG 2 cut(s) 1065, 1186
BtsIMutI CAGTG 2 cut(s) 164, 816
Cac8I GCNNGC 2 cut(s) 384, 904
CaiI CAGNNNCTG 1 cut(s) 1027
CfoI GCGC 3 cut(s) 14, 382, 1186
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 5 cut(s) 566, 667, 776, 923, 1076
Csp6I GTAC 1 cut(s) 191
CviAII CATG 6 cut(s) 60, 470, 592, 703, 1070, 1192
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 4 cut(s) 280, 651, 663, 968
DpnI GATC 8 cut(s) 285, 316, 444, 1013, 1020, 1097, 1156, 1272
DpnII GATC 8 cut(s) 283, 314, 442, 1011, 1018, 1095, 1154, 1270
DraI TTTAAA 1 cut(s) 879
Eam1104I CTCTTC 1 cut(s) 686
EarI CTCTTC 1 cut(s) 686
EciI GGCGGA 1 cut(s) 636
Eco130I CCWWGG 1 cut(s) 724
Eco47I GGWCC 4 cut(s) 566, 667, 923, 1076
Eco47III AGCGCT 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 840
EcoO109I RGGNCCY 1 cut(s) 667
EcoRII CCWGG 1 cut(s) 1135
EcoT14I CCWWGG 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 724
FaeI CATG 6 cut(s) 63, 473, 595, 706, 1073, 1195
FalI AAGNNNNNCTT 3 cut(s) 30, 813, 845
FatI CATG 6 cut(s) 59, 469, 591, 702, 1069, 1191
FbaI TGATCA 2 cut(s) 442, 1018
FblI GTMKAC 2 cut(s) 151, 243
Fnu4HI GCNGC 4 cut(s) 730, 790, 965, 1025
FokI GGATG 2 cut(s) 1052, 1173
Fsp4HI GCNGC 4 cut(s) 730, 790, 965, 1025
FspBI CTAG 6 cut(s) 96, 383, 725, 869, 903, 950
GlaI GCGC 3 cut(s) 13, 381, 1185
GluI GCNGC 4 cut(s) 730, 790, 965, 1025
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 2 cut(s) 777, 887
HapII CCGG 4 cut(s) 492, 564, 636, 1004
HhaI GCGC 3 cut(s) 14, 382, 1186
Hin1II CATG 6 cut(s) 63, 473, 595, 706, 1073, 1195
Hin6I GCGC 3 cut(s) 12, 380, 1184
HinP1I GCGC 3 cut(s) 12, 380, 1184
HincII GTYRAC 2 cut(s) 846, 918
HindII GTYRAC 2 cut(s) 846, 918
HindIII AAGCTT 1 cut(s) 24
HpaI GTTAAC 2 cut(s) 846, 918
HpaII CCGG 4 cut(s) 492, 564, 636, 1004
HphI GGTGA 4 cut(s) 268, 289, 625, 1021
Hpy166II GTNNAC 4 cut(s) 152, 244, 846, 918
Hpy188I TCNGA 4 cut(s) 283, 319, 654, 1203
Hpy188III TCNNGA 6 cut(s) 440, 665, 716, 950, 1099, 1265
Hpy8I GTNNAC 4 cut(s) 152, 244, 846, 918
HpyAV CCTTC 2 cut(s) 49, 722
HpyCH4III ACNGT 2 cut(s) 347, 933
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 597, 865
HpyF3I CTNAG 4 cut(s) 280, 651, 663, 968
Hsp92II CATG 6 cut(s) 63, 473, 595, 706, 1073, 1195
HspAI GCGC 3 cut(s) 12, 380, 1184
Ksp22I TGATCA 2 cut(s) 442, 1018
KspAI GTTAAC 2 cut(s) 846, 918
Kzo9I GATC 8 cut(s) 283, 314, 442, 1011, 1018, 1095, 1154, 1270
LmnI GCTCC 3 cut(s) 453, 737, 961
Lsp1109I GCAGC 4 cut(s) 741, 801, 976, 1011
LweI GCATC 3 cut(s) 664, 889, 1195
MaeI CTAG 6 cut(s) 96, 383, 725, 869, 903, 950
MaeIII GTNAC 3 cut(s) 347, 927, 1117
MalI GATC 8 cut(s) 285, 316, 444, 1013, 1020, 1097, 1156, 1272
MboI GATC 8 cut(s) 283, 314, 442, 1011, 1018, 1095, 1154, 1270
MflI RGATCY 2 cut(s) 283, 1270
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 5 cut(s) 260, 510, 625, 1030, 1057
MmeI TCCRAC 2 cut(s) 433, 504
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 6 cut(s) 35, 56, 602, 845, 878, 917
MspA1I CMGCKG 1 cut(s) 1024
MspI CCGG 4 cut(s) 492, 564, 636, 1004
MspR9I CCNGG 1 cut(s) 1137
Mva1269I GAATGC 1 cut(s) 1131
MvaI CCWGG 1 cut(s) 1137
MvnI CGCG 2 cut(s) 380, 1184
MwoI GCNNNNNNNGC 3 cut(s) 77, 597, 865
NdeII GATC 8 cut(s) 283, 314, 442, 1011, 1018, 1095, 1154, 1270
NheI GCTAGC 2 cut(s) 382, 902
NlaIII CATG 6 cut(s) 63, 473, 595, 706, 1073, 1195
NlaIV GGNNCC 3 cut(s) 496, 1002, 1272
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 2 cut(s) 347, 1117
NspI RCATGY 1 cut(s) 63
NspV TTCGAA 1 cut(s) 164
PctI GAATGC 1 cut(s) 1131
PdmI GAANNNNTTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 306
PkrI GCNGC 4 cut(s) 731, 791, 966, 1026
PpuMI RGGWCCY 1 cut(s) 667
Psp5II RGGWCCY 1 cut(s) 667
Psp6I CCWGG 1 cut(s) 1135
PspGI CCWGG 1 cut(s) 1135
PspN4I GGNNCC 3 cut(s) 496, 1002, 1272
PspPI GGNCC 5 cut(s) 566, 667, 776, 923, 1076
PspPPI RGGWCCY 1 cut(s) 667
PstI CTGCAG 1 cut(s) 1235
PstNI CAGNNNCTG 1 cut(s) 1027
PsuI RGATCY 2 cut(s) 283, 1270
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
SaqAI TTAA 6 cut(s) 35, 56, 602, 845, 878, 917
SatI GCNGC 4 cut(s) 730, 790, 965, 1025
Sau3AI GATC 8 cut(s) 283, 314, 442, 1011, 1018, 1095, 1154, 1270
Sau96I GGNCC 5 cut(s) 566, 667, 776, 923, 1076
ScrFI CCNGG 1 cut(s) 1137
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 3 cut(s) 664, 889, 1195
SfcI CTRYAG 1 cut(s) 1231
SfuI TTCGAA 1 cut(s) 164
SinI GGWCC 4 cut(s) 566, 667, 923, 1076
Sse9I AATT 5 cut(s) 260, 510, 625, 1030, 1057
SsiI CCGC 4 cut(s) 176, 372, 579, 647
SspMI CTAG 6 cut(s) 96, 383, 725, 869, 903, 950
StyD4I CCNGG 1 cut(s) 1135
StyI CCWWGG 1 cut(s) 724
TaaI ACNGT 2 cut(s) 347, 933
TaqI TCGA 2 cut(s) 164, 393
TasI AATT 5 cut(s) 260, 510, 625, 1030, 1057
Tru1I TTAA 6 cut(s) 35, 56, 602, 845, 878, 917
Tru9I TTAA 6 cut(s) 35, 56, 602, 845, 878, 917
TscAI CASTG 2 cut(s) 164, 823
TseFI GTSAC 2 cut(s) 347, 1117
TseI GCWGC 4 cut(s) 729, 789, 964, 1024
Tsp45I GTSAC 2 cut(s) 347, 1117
TspDTI ATGAA 4 cut(s) 608, 691, 1054, 1250
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 2 cut(s) 164, 823
Van91I CCANNNNNTGG 1 cut(s) 306
VpaK11BI GGWCC 4 cut(s) 566, 667, 923, 1076
XapI RAATTY 2 cut(s) 260, 1030
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 1 cut(s) 63
XmaJI CCTAGG 1 cut(s) 724
XmiI GTMKAC 2 cut(s) 151, 243
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 6 cut(s) 96, 383, 725, 869, 903, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.