Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003586

Physical Location & Seq

Genomic Coordinates Forward (+)

5783909 .. 5789100

Gene Structure 5192 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G09020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGCTTACTTCACCTTCTTCTTCTTCTACATATGATCCATTCTCTTCCAATTTCTCTCCGTCGCTCACTAATGCCTTCTCTAGTAGCTTCACCATTCCCATGGGATTGAAATTGAGTAGGAGAGTCACCAGAGCTCGGATCTTCTCTCGTAAGATTAAGGATCGTTCAACACTTAAGGTCACTTGTCGTCGCGCACACGAACGTGTGGTTGAGGAAGAAGCATCTACAATGACGGAGACGAAATTGTTTAAAGTTTCTTCTGGTGAGGTATCTCCTCTTGGTGTGTCACAAGTCGATAAGGGAATCAATTTTGCTTTGTTTTCTCAGAATGCTACTTCTGTCACACTCTGCTTATCACTATCCCAGAGTGGTAAGGATGATACGGATGATGATGGTATGATAGAGTTGGTTTTGGATCCCAGTGTAAACAAGACTGGAGATACTTGGCACATTTGTGTTGAGGATTTGCCTCTCAATAATGTTCTCTATGGTTACCGTGTTGATGGTCCTGGAGAATGGCAACAAGGGCATCGGTTTGATCGTAGTATTTTGCTTTTGGATCCCTATGCTAAGCTAGTTAAAGGTCATAGTTCTTTTGGAGATAGTAGTCAGAAGTTTGCTCAATTTTATGGAACTTATGACTTTGAGAGCTCGCCATTTGACTGGGGAGATGACTACAAGTTTCCTAACATACCTGAGAAGGATCTTGTTATTTATGAAATGAATGTTCGTGCTTTTACTGCTGATGAATCCAGTGGGATGGACCCAGCTATAGGAGGCAGTTACCTCGGTTTCATTGAGAAGATCCCACACCTTCAAGATCTGGGTATAAATGCAGTGGAATTATTGCCAGTGTTTGAGTTTGATGAACTGGAGCTTCAGAGGCGTTCTAACCCTAGGGATCACATGGTTAACACATGGGGTTACTCGACAGTTAACTTTTTTGCTCCGATGAGCCGATATGCTAGTGGTGAGGGAGACCCTATTAAAGCTTCGAAAGAGTTTAAAGAAATGGTCAAAGCCTTGCATTCTGCTGGTATAGAGGTCATTTTGGACGTAGTTTATAATCACACCAACGAAGCAGATGACAAATACCCTTATACCACTTCATTTCGTGGCATAGACAACAAGGTTTATTACATGCTTGATCCAAACAACCAACTGCTTAACTTTTCTGGCTGTGGGAATACACTGAACTGTAACCATCCAGTTGTTATGGAACTCATACTTGATAGCTTAAGGCACTGGGTCACTGAATATCACGTGGATGGTTTCCGGTTTGATCTTGCTAGTGTGCTTTGCCGAGCAACAGATGGATCTCCACTCAGTGCTCCCCCACTGATAAGGGCAATTGCCAAGGATTCTGTTCTGTCAAGATGTAAAATAATTGCAGAGCCTTGGGATTGTGGAGGATTATATCTAGTCGGGAAGTTTCCAAATTGGGATAGGTGGGCTGAGTGGAATGGGATGTACCGGGATGATGTTAGAAGATTTATCAAGGGTGACAGTGGCATGAAAGGAAGCTTTGCTACTCGGGTTTCTGGATCTTCCGATCTTTACCAGGTTAACCAGAGGAAGCCTTACCATGGTGTAAATTTTGTGATCGCACATGATGGATTCACATTGCGAGATCTTGTATCGTACAACTTTAAGCACAATGAGGCCAATGGAGAAGGAGGAAATGATGGATGTAATGACAATCATAGCTGGAACTGTGGTTTTGAAGGGGAAACTGGTGATGCTCACATCAAATCTTTACGTACTCGGCAAATGAAGAACTTTCATTTAGCTTTGATGATTTCTCAGGGAACACCAATGATGCTAATGGGAGACGAATATGGACATACACGGTATGGTAACAACAATAGCTACGGACATGATACCTCCCTTAACAATTTCCAGTGGAAAGAGCTCGACGCAAAGAAGCAGAACCATTTCAGGTTTTTCTCGGAGGTGATCAAGTTCCGACATTCACACCATGTACTCAAGCATGAAAATTTCCTCACCCAAGGTGAGATTACATGGCATGAAGATAATTGGGACAACAGTGAGAGCAAGTTCCTAGCTTTCACTCTCCATGACGGTATAGGTGGCCGTGACATCTATGTGGCATTCAACGCCCACGACTACTTTGTCAAGGCTCTGATTCCACAGCCACCACCGGGAAAACAATGGTTCCGTGTGGCTGACACAAACCTCGAGTCGCCGGATGATTTTGTGAGGGAAGGTGTGGCCGGTGTGGCTGATACCTACAATGTGGCCCCATTCTCTTCAATCCTTCTCCAGTCCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009657 GO:0009660 GO:0009987 GO:0015980 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0071840 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

764

Amino Acids

86.32

Weight (kDa)

5.66

Isoelectric Point (pI)

33.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	92 - 190	9.4e-12	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	254 - 435	3e-13	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	677 - 743	1.2e-10	Isoamylase 1-3, C-terminal
Isoamylase_C	PF21331	677 - 736	4.8e-06	Isoamylase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09020
fragaria_vesca	FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440
malus_domestica	MD08G1188900.v1.1 MD15G1376800.v1.1
prunus_persica	Prupe.1G523500_v2.0.a1 Prupe.1G523500_v2.0.a1
pyrus_communis	pycom08g16260 pycom15g33660
rosa_chinensis	RchiOBHm_Chr7g0238421
rosa_laevigata	RLG00000000911
rosa_multiflora	Rmu_sc0031277.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223020
rosa_rugosa	Rorug07G0312600
rosa_samantha	Rh7AG467500 Rh7BG438200 Rh7CG485500 Rh7DG454000
rosa_wichuraiana	Rw7G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1065
AasI	GACNNNNNNGTC	1 cut(s)	2132
AccII	CGCG	1 cut(s)	192
AcoI	YGGCCR	2 cut(s)	2092, 2232
AcsI	RAATTY	2 cut(s)	1594, 1996
AcuI	CTGAAG	1 cut(s)	863
AcvI	CACGTG	1 cut(s)	1264
AdeI	CACNNNGTG	1 cut(s)	1328
AfaI	GTAC	4 cut(s)	1472, 1643, 1762, 1983
AfiI	CCNNNNNNNGG	4 cut(s)	135, 773, 1586, 2162
AflII	CTTAAG	2 cut(s)	173, 1237
AflIII	ACRYGT	1 cut(s)	202
AgsI	TTSAA	6 cut(s)	109, 168, 818, 1724, 2116, 2274
AjnI	CCWGG	2 cut(s)	508, 1560
AleI	CACNNNNGTG	2 cut(s)	201, 453
Alw21I	GWGCWC	4 cut(s)	136, 653, 1333, 1914
Alw26I	GTCTC	3 cut(s)	230, 972, 1824
Ama87I	CYCGRG	2 cut(s)	1533, 2198
AoxI	GGCC	4 cut(s)	1662, 2092, 2232, 2259
ApoI	RAATTY	2 cut(s)	1594, 1996
ArsI	GACNNNNNNTTYG	2 cut(s)	2186, 2218
Asp700I	GAANNNNTTC	1 cut(s)	1934
AspA2I	CCTAGG	1 cut(s)	896
AspLEI	GCGC	1 cut(s)	194
AspS9I	GGNCC	3 cut(s)	506, 763, 2260
AsuC2I	CCSGG	2 cut(s)	1475, 2163
AsuII	TTCGAA	1 cut(s)	995
AvaI	CYCGRG	2 cut(s)	1533, 2198
AvaII	GGWCC	2 cut(s)	506, 763
AvrII	CCTAGG	1 cut(s)	896
BamHI	GGATCC	2 cut(s)	415, 559
BanII	GRGCYC	3 cut(s)	136, 653, 1914
BarI	GAAGNNNNNNTAC	2 cut(s)	1513, 1545
BbrPI	CACGTG	1 cut(s)	1264
Bbv12I	GWGCWC	4 cut(s)	136, 653, 1333, 1914
BccI	CCATC	8 cut(s)	386, 497, 754, 1212, 1262, 1307, 1607, 1679
BceAI	ACGGC	1 cut(s)	2079
BcgI	CGANNNNNNTGC	2 cut(s)	769, 803
BciT130I	CCWGG	2 cut(s)	510, 1562
BclI	TGATCA	1 cut(s)	1956
BcnI	CCSGG	2 cut(s)	1475, 2163
BcoDI	GTCTC	3 cut(s)	230, 972, 1824
BfaI	CTAG	7 cut(s)	81, 575, 897, 966, 1290, 1421, 2063
BfmI	CTRYAG	1 cut(s)	771
BfrI	CTTAAG	2 cut(s)	173, 1237
BglI	GCCNNNNNGGC	1 cut(s)	2240
BglII	AGATCT	2 cut(s)	820, 1630
BlnI	CCTAGG	1 cut(s)	896
BlpI	GCTNAGC	1 cut(s)	570
Bme1390I	CCNGG	4 cut(s)	510, 1475, 1562, 2163
Bme18I	GGWCC	2 cut(s)	506, 763
BmeT110I	CYCGRG	2 cut(s)	1533, 2198
BmgT120I	GGNCC	3 cut(s)	506, 763, 2260
BmiI	GGNNCC	5 cut(s)	417, 561, 765, 2177, 2262
BmrFI	CCNGG	4 cut(s)	510, 1475, 1562, 2163
BmrI	ACTGGG	3 cut(s)	414, 673, 1255
BmsI	GCATC	4 cut(s)	230, 538, 1729, 1809
BmuI	ACTGGG	3 cut(s)	414, 673, 1255
BpmI	CTGGAG	4 cut(s)	456, 531, 893, 2267
Bpu1102I	GCTNAGC	1 cut(s)	570
Bpu14I	TTCGAA	1 cut(s)	995
BpuEI	CTTGAG	1 cut(s)	1970
BpuMI	CCSGG	2 cut(s)	1475, 2163
BsaAI	YACGTR	2 cut(s)	1264, 1760
BsaI	GGTCTC	1 cut(s)	972
BsaJI	CCNNGG	7 cut(s)	99, 787, 896, 1356, 1397, 1585, 2008
BsaWI	WCCGGW	1 cut(s)	1275
Bsc4I	CCNNNNNNNGG	4 cut(s)	135, 773, 1586, 2162
Bse118I	RCCGGY	1 cut(s)	2234
Bse3DI	GCAATG	1 cut(s)	1622
BseBI	CCWGG	2 cut(s)	510, 1562
BseDI	CCNNGG	7 cut(s)	99, 787, 896, 1356, 1397, 1585, 2008
BseGI	GGATG	9 cut(s)	382, 391, 765, 1204, 1273, 1473, 1483, 1694, 2215
BseLI	CCNNNNNNNGG	4 cut(s)	135, 773, 1586, 2162
BseMI	GCAATG	1 cut(s)	1622
BseMII	CTCAG	5 cut(s)	338, 687, 1339, 1446, 1817
BseRI	GAGGAG	1 cut(s)	264
BseYI	CCCAGC	1 cut(s)	766
Bsh1236I	CGCG	1 cut(s)	192
BshFI	GGCC	4 cut(s)	1664, 2094, 2234, 2261
BsiHKAI	GWGCWC	4 cut(s)	136, 653, 1333, 1914
BsiHKCI	CYCGRG	2 cut(s)	1533, 2198
BsiSI	CCGG	5 cut(s)	1276, 1474, 2162, 2207, 2235
BslFI	GGGAC	1 cut(s)	2054
BslI	CCNNNNNNNGG	4 cut(s)	135, 773, 1586, 2162
BsmAI	GTCTC	3 cut(s)	230, 972, 1824
BsmBI	CGTCTC	2 cut(s)	230, 1824
BsmFI	GGGAC	1 cut(s)	2054
BsmI	GAATGC	3 cut(s)	334, 1027, 2111
BsnI	GGCC	4 cut(s)	1664, 2094, 2234, 2261
Bso31I	GGTCTC	1 cut(s)	972
BsoBI	CYCGRG	2 cut(s)	1533, 2198
Bsp119I	TTCGAA	1 cut(s)	995
Bsp1286I	GDGCHC	4 cut(s)	136, 653, 1333, 1914
Bsp1720I	GCTNAGC	1 cut(s)	570
Bsp19I	CCATGG	2 cut(s)	99, 1585
BspANI	GGCC	4 cut(s)	1664, 2094, 2234, 2261
BspCNI	CTCAG	5 cut(s)	337, 688, 1338, 1447, 1816
BspFNI	CGCG	1 cut(s)	192
BspLI	GGNNCC	5 cut(s)	417, 561, 765, 2177, 2262
BspT104I	TTCGAA	1 cut(s)	995
BspTI	CTTAAG	2 cut(s)	173, 1237
BspTNI	GGTCTC	1 cut(s)	972
BsrDI	GCAATG	1 cut(s)	1622
BsrFI	RCCGGY	1 cut(s)	2234
BssAI	RCCGGY	1 cut(s)	2234
BssECI	CCNNGG	7 cut(s)	99, 787, 896, 1356, 1397, 1585, 2008
BssT1I	CCWWGG	6 cut(s)	99, 896, 1356, 1397, 1585, 2008
Bst2UI	CCWGG	2 cut(s)	510, 1562
Bst4CI	ACNGT	8 cut(s)	497, 934, 1199, 1508, 1715, 1851, 2048, 2084
Bst6I	CTCTTC	2 cut(s)	49, 2275
BstAFI	CTTAAG	2 cut(s)	173, 1237
BstBAI	YACGTR	2 cut(s)	1264, 1760
BstBI	TTCGAA	1 cut(s)	995
BstC8I	GCNNGC	1 cut(s)	653
BstDEI	CTNAG	6 cut(s)	324, 570, 696, 1325, 1455, 1803
BstDSI	CCRYGG	2 cut(s)	99, 1585
BstEII	GGTNACC	1 cut(s)	491
BstF5I	GGATG	9 cut(s)	382, 391, 765, 1204, 1273, 1473, 1483, 1694, 2215
BstFNI	CGCG	1 cut(s)	192
BstHHI	GCGC	1 cut(s)	194
BstMAI	GTCTC	3 cut(s)	230, 972, 1824
BstMWI	GCNNNNNNNGC	5 cut(s)	526, 740, 883, 2117, 2240
BstNI	CCWGG	2 cut(s)	510, 1562
BstNSI	RCATGY	1 cut(s)	1144
BstPI	GGTNACC	1 cut(s)	491
BstSCI	CCNGG	4 cut(s)	508, 1473, 1560, 2161
BstSFI	CTRYAG	1 cut(s)	771
BstSNI	TACGTA	1 cut(s)	1760
BstUI	CGCG	1 cut(s)	192
BstX2I	RGATCY	9 cut(s)	138, 415, 559, 703, 804, 820, 1316, 1544, 1630
BstXI	CCANNNNNNTGG	3 cut(s)	100, 663, 760
BstYI	RGATCY	9 cut(s)	138, 415, 559, 703, 804, 820, 1316, 1544, 1630
BsuRI	GGCC	4 cut(s)	1664, 2094, 2234, 2261
BtgI	CCRYGG	2 cut(s)	99, 1585
BtsCI	GGATG	9 cut(s)	382, 391, 765, 1204, 1273, 1473, 1483, 1694, 2215
BtsI	GCAGTG	1 cut(s)	843
Cac8I	GCNNGC	1 cut(s)	653
CfoI	GCGC	1 cut(s)	194
Cfr10I	RCCGGY	1 cut(s)	2234
Cfr13I	GGNCC	3 cut(s)	506, 763, 2260
CseI	GACGC	1 cut(s)	1925
CsiI	ACCWGGT	1 cut(s)	1560
Csp6I	GTAC	4 cut(s)	1471, 1642, 1761, 1982
CviQI	GTAC	4 cut(s)	1471, 1642, 1761, 1982
DdeI	CTNAG	6 cut(s)	324, 570, 696, 1325, 1455, 1803
DraI	TTTAAA	2 cut(s)	250, 1006
DraIII	CACNNNGTG	1 cut(s)	1328
DrdI	GACNNNNNNGTC	1 cut(s)	2132
DseDI	GACNNNNNNGTC	1 cut(s)	2132
EaeI	YGGCCR	2 cut(s)	2092, 2232
Eam1104I	CTCTTC	2 cut(s)	49, 2275
EarI	CTCTTC	2 cut(s)	49, 2275
Ecl136II	GAGCTC	3 cut(s)	134, 651, 1912
Eco105I	TACGTA	1 cut(s)	1760
Eco130I	CCWWGG	6 cut(s)	99, 896, 1356, 1397, 1585, 2008
Eco24I	GRGCYC	3 cut(s)	136, 653, 1914
Eco31I	GGTCTC	1 cut(s)	972
Eco47I	GGWCC	2 cut(s)	506, 763
Eco53kI	GAGCTC	3 cut(s)	134, 651, 1912
Eco57I	CTGAAG	1 cut(s)	863
Eco72I	CACGTG	1 cut(s)	1264
Eco88I	CYCGRG	2 cut(s)	1533, 2198
Eco91I	GGTNACC	1 cut(s)	491
EcoICRI	GAGCTC	3 cut(s)	134, 651, 1912
EcoO65I	GGTNACC	1 cut(s)	491
EcoRII	CCWGG	2 cut(s)	508, 1560
EcoT14I	CCWWGG	6 cut(s)	99, 896, 1356, 1397, 1585, 2008
EcoT38I	GRGCYC	3 cut(s)	136, 653, 1914
ErhI	CCWWGG	6 cut(s)	99, 896, 1356, 1397, 1585, 2008
Esp3I	CGTCTC	2 cut(s)	230, 1824
FaqI	GGGAC	1 cut(s)	2054
FauNDI	CATATG	1 cut(s)	31
FbaI	TGATCA	1 cut(s)	1956
FokI	GGATG	9 cut(s)	389, 398, 772, 1191, 1280, 1480, 1490, 1701, 2222
FriOI	GRGCYC	3 cut(s)	136, 653, 1914
FspBI	CTAG	7 cut(s)	81, 575, 897, 966, 1290, 1421, 2063
GlaI	GCGC	1 cut(s)	193
GsaI	CCCAGC	1 cut(s)	770
GsuI	CTGGAG	4 cut(s)	456, 531, 893, 2267
HaeIII	GGCC	4 cut(s)	1664, 2094, 2234, 2261
HapII	CCGG	5 cut(s)	1276, 1474, 2162, 2207, 2235
HgaI	GACGC	1 cut(s)	1925
HhaI	GCGC	1 cut(s)	194
Hin6I	GCGC	1 cut(s)	192
HinP1I	GCGC	1 cut(s)	192
HincII	GTYRAC	3 cut(s)	913, 937, 1567
HindII	GTYRAC	3 cut(s)	913, 937, 1567
HindIII	AAGCTT	2 cut(s)	990, 1522
HinfI	GANTC	7 cut(s)	123, 303, 749, 1361, 1617, 2146, 2201
HpaI	GTTAAC	3 cut(s)	913, 937, 1567
HpaII	CCGG	5 cut(s)	1276, 1474, 2162, 2207, 2235
Hpy166II	GTNNAC	4 cut(s)	427, 913, 937, 1567
Hpy188I	TCNGA	9 cut(s)	138, 327, 612, 882, 951, 1552, 1951, 1967, 2145
Hpy188III	TCNNGA	4 cut(s)	818, 1374, 1426, 1542
Hpy8I	GTNNAC	4 cut(s)	427, 913, 937, 1567
Hpy99I	CGWCG	3 cut(s)	64, 192, 1919
HpyAV	CCTTC	8 cut(s)	24, 85, 694, 824, 1667, 1718, 2219, 2288
HpyCH4III	ACNGT	8 cut(s)	497, 934, 1199, 1508, 1715, 1851, 2048, 2084
HpyCH4IV	ACGT	4 cut(s)	202, 1056, 1263, 1759
HpyCH4V	TGCA	3 cut(s)	836, 1027, 1391
HpyF10VI	GCNNNNNNNGC	5 cut(s)	526, 740, 883, 2117, 2240
HpyF3I	CTNAG	6 cut(s)	324, 570, 696, 1325, 1455, 1803
HpySE526I	ACGT	4 cut(s)	202, 1056, 1263, 1759
HspAI	GCGC	1 cut(s)	192
Ksp22I	TGATCA	1 cut(s)	1956
KspAI	GTTAAC	3 cut(s)	913, 937, 1567
LmnI	GCTCC	3 cut(s)	874, 952, 1336
LweI	GCATC	4 cut(s)	230, 538, 1729, 1809
MabI	ACCWGGT	1 cut(s)	1560
MaeI	CTAG	7 cut(s)	81, 575, 897, 966, 1290, 1421, 2063
MaeII	ACGT	4 cut(s)	202, 1056, 1263, 1759
MfeI	CAATTG	1 cut(s)	1350
MflI	RGATCY	9 cut(s)	138, 415, 559, 703, 804, 820, 1316, 1544, 1630
MhlI	GDGCHC	4 cut(s)	136, 653, 1333, 1914
MlyI	GAGTC	2 cut(s)	132, 2210
MmeI	TCCRAC	1 cut(s)	1990
MroXI	GAANNNNTTC	1 cut(s)	1934
MslI	CAYNNNNRTG	5 cut(s)	98, 201, 453, 1266, 2105
MspCI	CTTAAG	2 cut(s)	173, 1237
MspI	CCGG	5 cut(s)	1276, 1474, 2162, 2207, 2235
MspR9I	CCNGG	4 cut(s)	510, 1475, 1562, 2163
MunI	CAATTG	1 cut(s)	1350
Mva1269I	GAATGC	3 cut(s)	334, 1027, 2111
MvaI	CCWGG	2 cut(s)	510, 1562
MvnI	CGCG	1 cut(s)	192
MwoI	GCNNNNNNNGC	5 cut(s)	526, 740, 883, 2117, 2240
NciI	CCSGG	2 cut(s)	1475, 2163
NcoI	CCATGG	2 cut(s)	99, 1585
NdeI	CATATG	1 cut(s)	31
NlaIV	GGNNCC	5 cut(s)	417, 561, 765, 2177, 2262
NmeAIII	GCCGAG	2 cut(s)	1328, 1744
NmuCI	GTSAC	7 cut(s)	124, 178, 285, 340, 1249, 1502, 2096
NspI	RCATGY	1 cut(s)	1144
NspV	TTCGAA	1 cut(s)	995
OliI	CACNNNNGTG	2 cut(s)	201, 453
PaeR7I	CTCGAG	1 cut(s)	2198
PcsI	WCGNNNNNNNCGW	1 cut(s)	538
PctI	GAATGC	3 cut(s)	334, 1027, 2111
PdmI	GAANNNNTTC	1 cut(s)	1934
PfeI	GAWTC	5 cut(s)	303, 749, 1361, 1617, 2146
PfoI	TCCNGGA	1 cut(s)	508
PleI	GAGTC	2 cut(s)	131, 2209
PmaCI	CACGTG	1 cut(s)	1264
PmlI	CACGTG	1 cut(s)	1264
PpsI	GAGTC	2 cut(s)	131, 2209
Ppu21I	YACGTR	2 cut(s)	1264, 1760
PsiI	TTATAA	1 cut(s)	1065
Psp124BI	GAGCTC	3 cut(s)	136, 653, 1914
Psp6I	CCWGG	2 cut(s)	508, 1560
PspCI	CACGTG	1 cut(s)	1264
PspEI	GGTNACC	1 cut(s)	491
PspFI	CCCAGC	1 cut(s)	766
PspGI	CCWGG	2 cut(s)	508, 1560
PspN4I	GGNNCC	5 cut(s)	417, 561, 765, 2177, 2262
PspPI	GGNCC	3 cut(s)	506, 763, 2260
PspXI	VCTCGAGB	1 cut(s)	2198
PsuI	RGATCY	9 cut(s)	138, 415, 559, 703, 804, 820, 1316, 1544, 1630
RsaI	GTAC	4 cut(s)	1472, 1643, 1762, 1983
RsaNI	GTAC	4 cut(s)	1471, 1642, 1761, 1982
RseI	CAYNNNNRTG	5 cut(s)	98, 201, 453, 1266, 2105
SacI	GAGCTC	3 cut(s)	136, 653, 1914
Sau96I	GGNCC	3 cut(s)	506, 763, 2260
SchI	GAGTC	2 cut(s)	132, 2210
ScrFI	CCNGG	4 cut(s)	510, 1475, 1562, 2163
SduI	GDGCHC	4 cut(s)	136, 653, 1333, 1914
SexAI	ACCWGGT	1 cut(s)	1560
SfaNI	GCATC	4 cut(s)	230, 538, 1729, 1809
SfcI	CTRYAG	1 cut(s)	771
Sfr274I	CTCGAG	1 cut(s)	2198
SfuI	TTCGAA	1 cut(s)	995
SinI	GGWCC	2 cut(s)	506, 763
SlaI	CTCGAG	1 cut(s)	2198
SmiMI	CAYNNNNRTG	5 cut(s)	98, 201, 453, 1266, 2105
SmlI	CTYRAG	4 cut(s)	173, 1237, 1985, 2198
SmoI	CTYRAG	4 cut(s)	173, 1237, 1985, 2198
SnaBI	TACGTA	1 cut(s)	1760
SspMI	CTAG	7 cut(s)	81, 575, 897, 966, 1290, 1421, 2063
SstI	GAGCTC	3 cut(s)	136, 653, 1914
StyD4I	CCNGG	4 cut(s)	508, 1473, 1560, 2161
StyI	CCWWGG	6 cut(s)	99, 896, 1356, 1397, 1585, 2008
TaaI	ACNGT	8 cut(s)	497, 934, 1199, 1508, 1715, 1851, 2048, 2084
TaiI	ACGT	4 cut(s)	205, 1059, 1266, 1762
TaqI	TCGA	5 cut(s)	294, 929, 995, 1914, 2199
TatI	WGTACW	1 cut(s)	1981
TfiI	GAWTC	5 cut(s)	303, 749, 1361, 1617, 2146
TseFI	GTSAC	7 cut(s)	124, 178, 285, 340, 1249, 1502, 2096
Tsp45I	GTSAC	7 cut(s)	124, 178, 285, 340, 1249, 1502, 2096
TspGWI	ACGGA	5 cut(s)	48, 248, 398, 1887, 2168
Vha464I	CTTAAG	2 cut(s)	173, 1237
VpaK11BI	GGWCC	2 cut(s)	506, 763
XapI	RAATTY	2 cut(s)	1594, 1996
XceI	RCATGY	1 cut(s)	1144
XhoI	CTCGAG	1 cut(s)	2198
XmaJI	CCTAGG	1 cut(s)	896
XmnI	GAANNNNTTC	1 cut(s)	1934
XspI	CTAG	7 cut(s)	81, 575, 897, 966, 1290, 1421, 2063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.