Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0003586

Physical Location & Seq

Genomic Coordinates Reverse (-)

50409829 .. 50422182

Gene Structure 12354 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG438200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGAAACTGTGCCATTATCAACATCATCATAATCAGAATAGCAATCGAGAAAGATCAAAGATGCTCCTGCGTACCCCACATTTGTACGATTCAAATGTCTCCAAATTCCCGCTCTTTGCATCATCACCACCATTTTCGACGACTCAGGCCGGTGCTTCACCGTGTATAGTAGCTTCCCCTGTTCTTGATGTTGGGTTGCAGTTGAGTAAACAAGCGACAGGCAGCAATCAGCAAGGGCTTGTGAGAAAGACCCGGGATCATGTCATGCATAGCACGGGTAATGCGTACGGTCAGGGTGCTCAGGGAAGTGTGCTAGAGGAAGAAGCCCAACTGGCAGAGACCAGCCAGTCATGGAAAACTTTTCCAGGTCATGCTTTTCCGCTGGGTGTATCAGAGGTTGATACTGGTGTCAATTTTGCCATTTTCTCACAGCACGCAACCGCCGTCACACTTTGCATCAAACTTGTAGAGAGGGGACAGTTTGGAGGGCCAGATGGTGGAATGACTGAGCTGGCTTTGGATCCTAAAGAGAACAAGACAGGTGACATTTGGCACATATGTATAAAGGATTTTCCCTGTAGCAATGTCCTCTATGGTTACCGTATTGATGGGCCTCGAGGTTGGCAGCAAGGGCATCGGTTTGACAGCAGCATTGTACTAATAGATCCTTATGCGAAGCTTGTTGAAGGTCGCCGGTATTTTGGAGATTCCAGAGAAAAGTTATCTGGGTTTCTTGGTACTTATGATTTTGATAGCTTGCCTTTTGATTGGGGAGACAACTACAAGCTTCCAAATATACCCGAGAATGATCTTGTTATATATGAAATGAATGTTCGTGCATTTACAGCTGATGAATCCAGTGGTCTGGATCCAGATGTTCGTGGTAGCTACCGTGGTCTGATTGACAAGATTCCACACCTTCTAGAGCTCGGCATCAATGCTGTAGAGTTGCTGCCTGTCGTTGAATTTGATGAGTTTGAGTTTCAAAGGCGTTCAAATCCTAGAGATCACATGATTAATACATGGGGCTACTCAACAATAAACTTCTTTGCCCCTATGAGCCGTTATGCGAGTGCTGGTGGAGGACCCCTTAAAGCTTCCCATGAATTTAAAGAAATGGTTAAAGCCTTGCATGGTGCTGGGATAGAGGTCATTTTGGATGTTGTCTATAACCATACAAATGAAGCTGATGATGCCTACCCTTATACCACTTCATTTCGTGGCATAGATAATAAGGTTTATTACATGCTGGACCCAAACAATGGCCAATTACTGAACTTCTCGGGCTGTGGGAACTCATTAAACTGTAACCATCCTGTTGTTATGGAGCTCGTTCTCGAGAGCTTAAGACACTGGGTCACTGAATATCATGTGGATGGATTTAGATTTGACCTTGCTAGTGCTCTTTGTCGGGGAACAGATGGAGCTCCACTTGATGCACCGCCACTTATCCGGGCTATCGCAAAAGATGCCATTCTATCGAGATGTAAAGTTATTTCTGAGCCTTGGGATTGTGGAGGCCTTTATCTTGTTGGAAGATTTCCAAACTGGGACAGGTGGGCCGAATGGAATGGAATATATCGTGACGACTTAAGGAGATTTATAAAGGGAGACTCAGGTATGAAGGGAAGTTTTGCAACTCGTATTTCTGGATCTGCTGACCTTTACAAAACGAATAAGCGCAAGCCTTATCACAGTATTAATTTTATTATTGCACATGATGGGTTCACACTATATGATCTCGTTTCATACAATTTCAAGCACAATGATGCTAATGGAGAAGGAGGAAATGATGGATGTAATGATAATCTGAGCTGGAATTGTGGTTATGAAGGAGAAACCGATGATGCTACTATTAAAGCTATACGCTCCCGGCAAATGAAAAACTTTCATCTGGCGTTAATGATCTCTCAGGGAACACCAATGATGCTGATGGGAGATGAATATGGGCATACTCGATATGGAAATAACAACAGTTATGGACATGATACTGCTATTAACCATTTCCAGTGGGGACTGTTGGAATCACGGAAGAATAATCACTTCAGGTTTTTCAAAGAGGTGATAAACTATCGAAGAACACATCGTGTATTTGGTCGTGATACATTTCTCGAGAAAAGTGATGTGACATGGCATGAAGGCAACTGGGGCAATTATGAGAGCAAGTTTCTTGCATTTACGTTTCATGACAATAATGGAGGAGACATCTACATAGCATTCAATGCTCATGCTTATTTTATTGAAGTTCCGATCCCTTCACCACCAGAAAGGAGGATATGGTTTCGTGTGGTCGACACTAATCTCGAGTCTCCCAACGACTTTATGCTTGAAGGCGTCCCAGGCATCAGAGGCACTTACAACATGGCCCCTTACTCCTCAATTCTTCTTGAAGCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009657 GO:0009660 GO:0009987 GO:0015980 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0071840 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

799

Amino Acids

90.18

Weight (kDa)

5.95

Isoelectric Point (pI)

30.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	127 - 225	3.8e-11	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	290 - 417	8.8e-09	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	713 - 778	6.2e-12	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09020
fragaria_vesca	FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440
malus_domestica	MD08G1188900.v1.1 MD15G1376800.v1.1
prunus_persica	Prupe.1G523500_v2.0.a1 Prupe.1G523500_v2.0.a1
pyrus_communis	pycom08g16260 pycom15g33660
rosa_chinensis	RchiOBHm_Chr7g0238421
rosa_laevigata	RLG00000000911
rosa_multiflora	Rmu_sc0031277.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223020
rosa_rugosa	Rorug07G0312600
rosa_samantha	Rh7AG467500 Rh7BG438200 Rh7CG485500 Rh7DG454000
rosa_wichuraiana	Rw7G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1604
AasI	GACNNNNNNGTC	1 cut(s)	346
AbsI	CCTCGAGG	1 cut(s)	615
AccB7I	CCANNNNNTGG	2 cut(s)	497, 1262
AccBSI	CCGCTC	1 cut(s)	112
AccI	GTMKAC	1 cut(s)	2292
AciI	CCGC	4 cut(s)	110, 380, 441, 1442
AclWI	GGATC	8 cut(s)	264, 515, 528, 659, 863, 876, 1660, 2245
AcoI	YGGCCR	1 cut(s)	1264
AcsI	RAATTY	3 cut(s)	104, 965, 1106
AcuI	CTGAAG	1 cut(s)	2029
AcyI	GRCGYC	1 cut(s)	2334
AdeI	CACNNNGTG	1 cut(s)	2087
AfaI	GTAC	5 cut(s)	73, 86, 287, 657, 739
AfiI	CCNNNNNNNGG	2 cut(s)	497, 1262
AflII	CTTAAG	2 cut(s)	1345, 1591
AhdI	GACNNNNNGTC	1 cut(s)	1355
AjnI	CCWGG	2 cut(s)	364, 2338
Alw21I	GWGCWC	5 cut(s)	301, 930, 1332, 1405, 1429
Alw26I	GTCTC	6 cut(s)	103, 332, 768, 1605, 2196, 2313
AlwI	GGATC	8 cut(s)	264, 515, 528, 659, 863, 876, 1660, 2245
Ama87I	CYCGRG	7 cut(s)	252, 615, 800, 1282, 1337, 2111, 2303
AoxI	GGCC	7 cut(s)	147, 488, 611, 1264, 1519, 1560, 2364
ApeKI	GCWGC	4 cut(s)	222, 625, 648, 952
ApoI	RAATTY	3 cut(s)	104, 965, 1106
AseI	ATTAAT	2 cut(s)	1017, 1701
AspLEI	GCGC	1 cut(s)	1683
AspS9I	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
AsuC2I	CCSGG	4 cut(s)	253, 254, 1454, 1873
AsuHPI	GGTGA	5 cut(s)	117, 150, 554, 2074, 2250
AvaI	CYCGRG	7 cut(s)	252, 615, 800, 1282, 1337, 2111, 2303
AvaII	GGWCC	2 cut(s)	1085, 1252
BaeI	ACNNNNGTAYC	2 cut(s)	393, 426
BalI	TGGCCA	1 cut(s)	1266
BamHI	GGATCC	2 cut(s)	520, 868
BanII	GRGCYC	3 cut(s)	930, 1332, 1429
BarI	GAAGNNNNNNTAC	2 cut(s)	157, 189
Bbv12I	GWGCWC	5 cut(s)	301, 930, 1332, 1405, 1429
BbvI	GCAGC	4 cut(s)	234, 637, 660, 939
BccI	CCATC	8 cut(s)	488, 602, 1320, 1370, 1415, 1715, 1787, 1927
BceAI	ACGGC	2 cut(s)	428, 1047
BcgI	CGANNNNNNTGC	2 cut(s)	617, 651
BciT130I	CCWGG	2 cut(s)	366, 2340
BcnI	CCSGG	4 cut(s)	253, 254, 1454, 1873
BcoDI	GTCTC	6 cut(s)	103, 332, 768, 1605, 2196, 2313
BfaI	CTAG	4 cut(s)	314, 923, 1002, 1398
BfmI	CTRYAG	2 cut(s)	577, 942
BfrI	CTTAAG	2 cut(s)	1345, 1591
BglI	GCCNNNNNGGC	1 cut(s)	332
BisI	GCNGC	4 cut(s)	223, 626, 649, 953
BlsI	GCNGC	4 cut(s)	224, 627, 650, 954
Bme1390I	CCNGG	6 cut(s)	253, 254, 366, 1454, 1873, 2340
Bme18I	GGWCC	2 cut(s)	1085, 1252
BmeRI	GACNNNNNGTC	1 cut(s)	1355
BmeT110I	CYCGRG	7 cut(s)	252, 615, 800, 1282, 1337, 2111, 2303
BmgT120I	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
BmiI	GGNNCC	5 cut(s)	522, 870, 1087, 1254, 2367
BmrFI	CCNGG	6 cut(s)	253, 254, 366, 1454, 1873, 2340
BmrI	ACTGGG	3 cut(s)	1363, 1558, 2155
BmuI	ACTGGG	3 cut(s)	1363, 1558, 2155
Bpu10I	CCTNAGC	1 cut(s)	300
BpuMI	CCSGG	4 cut(s)	253, 254, 1454, 1873
BsaHI	GRCGYC	1 cut(s)	2334
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	4 cut(s)	252, 892, 1505, 2338
Bsc4I	CCNNNNNNNGG	2 cut(s)	497, 1262
Bse118I	RCCGGY	2 cut(s)	149, 693
Bse1I	ACTGG	8 cut(s)	336, 346, 409, 858, 1358, 1553, 2008, 2150
Bse3DI	GCAATG	1 cut(s)	589
BseBI	CCWGG	2 cut(s)	366, 2340
BseDI	CCNNGG	4 cut(s)	252, 892, 1505, 2338
BseGI	GGATG	4 cut(s)	1165, 1312, 1381, 1802
BseLI	CCNNNNNNNGG	2 cut(s)	497, 1262
BseMI	GCAATG	1 cut(s)	589
BseMII	CTCAG	7 cut(s)	158, 314, 498, 1491, 1629, 1802, 1925
BseNI	ACTGG	8 cut(s)	336, 346, 409, 858, 1358, 1553, 2008, 2150
BseRI	GAGGAG	2 cut(s)	2214, 2365
BseXI	GCAGC	4 cut(s)	234, 637, 660, 939
BseYI	CCCAGC	2 cut(s)	382, 1139
BshFI	GGCC	7 cut(s)	149, 490, 613, 1266, 1521, 1562, 2366
BsiHKAI	GWGCWC	5 cut(s)	301, 930, 1332, 1405, 1429
BsiHKCI	CYCGRG	7 cut(s)	252, 615, 800, 1282, 1337, 2111, 2303
BsiSI	CCGG	5 cut(s)	150, 253, 694, 1453, 1873
BsiWI	CGTACG	1 cut(s)	285
BslFI	GGGAC	4 cut(s)	489, 1565, 2028, 2321
BslI	CCNNNNNNNGG	2 cut(s)	497, 1262
BsmAI	GTCTC	6 cut(s)	103, 332, 768, 1605, 2196, 2313
BsmFI	GGGAC	4 cut(s)	489, 1565, 2028, 2321
BsmI	GAATGC	1 cut(s)	2216
BsnI	GGCC	7 cut(s)	149, 490, 613, 1266, 1521, 1562, 2366
Bso31I	GGTCTC	1 cut(s)	332
BsoBI	CYCGRG	7 cut(s)	252, 615, 800, 1282, 1337, 2111, 2303
Bsp1286I	GDGCHC	5 cut(s)	301, 930, 1332, 1405, 1429
BspACI	CCGC	4 cut(s)	110, 380, 441, 1442
BspANI	GGCC	7 cut(s)	149, 490, 613, 1266, 1521, 1562, 2366
BspCNI	CTCAG	7 cut(s)	157, 313, 499, 1492, 1628, 1803, 1924
BspHI	TCATGA	1 cut(s)	2185
BspLI	GGNNCC	5 cut(s)	522, 870, 1087, 1254, 2367
BspPI	GGATC	8 cut(s)	264, 515, 528, 659, 863, 876, 1660, 2245
BspTI	CTTAAG	2 cut(s)	1345, 1591
BspTNI	GGTCTC	1 cut(s)	332
BsrBI	CCGCTC	1 cut(s)	112
BsrDI	GCAATG	1 cut(s)	589
BsrFI	RCCGGY	2 cut(s)	149, 693
BsrI	ACTGG	8 cut(s)	336, 346, 409, 858, 1358, 1553, 2008, 2150
BssAI	RCCGGY	2 cut(s)	149, 693
BssECI	CCNNGG	4 cut(s)	252, 892, 1505, 2338
BssNI	GRCGYC	1 cut(s)	2334
BssT1I	CCWWGG	1 cut(s)	1505
Bst2UI	CCWGG	2 cut(s)	366, 2340
BstACI	GRCGYC	1 cut(s)	2334
BstAFI	CTTAAG	2 cut(s)	1345, 1591
BstAPI	GCANNNNNTGC	2 cut(s)	1469, 2222
BstC8I	GCNNGC	4 cut(s)	435, 513, 758, 1685
BstDEI	CTNAG	7 cut(s)	144, 300, 507, 1500, 1615, 1811, 1911
BstDSI	CCRYGG	1 cut(s)	892
BstEII	GGTNACC	1 cut(s)	596
BstF5I	GGATG	4 cut(s)	1165, 1312, 1381, 1802
BstHHI	GCGC	1 cut(s)	1683
BstMAI	GTCTC	6 cut(s)	103, 332, 768, 1605, 2196, 2313
BstMWI	GCNNNNNNNGC	9 cut(s)	332, 631, 845, 1193, 1469, 2148, 2222, 2340, 2349
BstNI	CCWGG	2 cut(s)	366, 2340
BstNSI	RCATGY	1 cut(s)	1249
BstPI	GGTNACC	1 cut(s)	596
BstSCI	CCNGG	6 cut(s)	251, 252, 364, 1452, 1871, 2338
BstSFI	CTRYAG	2 cut(s)	577, 942
BstV1I	GCAGC	4 cut(s)	234, 637, 660, 939
BstX2I	RGATCY	4 cut(s)	520, 664, 868, 1652
BstXI	CCANNNNNNTGG	1 cut(s)	865
BstYI	RGATCY	4 cut(s)	520, 664, 868, 1652
BsuRI	GGCC	7 cut(s)	149, 490, 613, 1266, 1521, 1562, 2366
BtgI	CCRYGG	1 cut(s)	892
BtsCI	GGATG	4 cut(s)	1165, 1312, 1381, 1802
BtsIMutI	CAGTG	4 cut(s)	865, 1351, 1359, 2015
Cac8I	GCNNGC	4 cut(s)	435, 513, 758, 1685
CciI	TCATGA	1 cut(s)	2185
CfoI	GCGC	1 cut(s)	1683
Cfr10I	RCCGGY	2 cut(s)	149, 693
Cfr13I	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
Cfr9I	CCCGGG	1 cut(s)	252
CseI	GACGC	1 cut(s)	2323
Csp6I	GTAC	5 cut(s)	72, 85, 286, 656, 738
CviQI	GTAC	5 cut(s)	72, 85, 286, 656, 738
DdeI	CTNAG	7 cut(s)	144, 300, 507, 1500, 1615, 1811, 1911
DraI	TTTAAA	1 cut(s)	1111
DraIII	CACNNNGTG	1 cut(s)	2087
DrdI	GACNNNNNNGTC	1 cut(s)	346
DriI	GACNNNNNGTC	1 cut(s)	1355
DseDI	GACNNNNNNGTC	1 cut(s)	346
EaeI	YGGCCR	1 cut(s)	1264
Eam1105I	GACNNNNNGTC	1 cut(s)	1355
Ecl136II	GAGCTC	3 cut(s)	928, 1330, 1427
Eco130I	CCWWGG	1 cut(s)	1505
Eco147I	AGGCCT	1 cut(s)	1521
Eco24I	GRGCYC	3 cut(s)	930, 1332, 1429
Eco31I	GGTCTC	1 cut(s)	332
Eco47I	GGWCC	2 cut(s)	1085, 1252
Eco53kI	GAGCTC	3 cut(s)	928, 1330, 1427
Eco57I	CTGAAG	1 cut(s)	2029
Eco88I	CYCGRG	7 cut(s)	252, 615, 800, 1282, 1337, 2111, 2303
Eco91I	GGTNACC	1 cut(s)	596
EcoICRI	GAGCTC	3 cut(s)	928, 1330, 1427
EcoO109I	RGGNCCY	1 cut(s)	1085
EcoO65I	GGTNACC	1 cut(s)	596
EcoRII	CCWGG	2 cut(s)	364, 2338
EcoT14I	CCWWGG	1 cut(s)	1505
EcoT22I	ATGCAT	1 cut(s)	270
EcoT38I	GRGCYC	3 cut(s)	930, 1332, 1429
ErhI	CCWWGG	1 cut(s)	1505
FaqI	GGGAC	4 cut(s)	489, 1565, 2028, 2321
FauI	CCCGC	1 cut(s)	117
FauNDI	CATATG	1 cut(s)	557
FblI	GTMKAC	1 cut(s)	2292
Fnu4HI	GCNGC	4 cut(s)	223, 626, 649, 953
FokI	GGATG	4 cut(s)	1172, 1299, 1388, 1809
FriOI	GRGCYC	3 cut(s)	930, 1332, 1429
Fsp4HI	GCNGC	4 cut(s)	223, 626, 649, 953
FspBI	CTAG	4 cut(s)	314, 923, 1002, 1398
GlaI	GCGC	1 cut(s)	1682
GluI	GCNGC	4 cut(s)	223, 626, 649, 953
GsaI	CCCAGC	2 cut(s)	386, 1143
HaeIII	GGCC	7 cut(s)	149, 490, 613, 1266, 1521, 1562, 2366
HapII	CCGG	5 cut(s)	150, 253, 694, 1453, 1873
HgaI	GACGC	1 cut(s)	2323
HhaI	GCGC	1 cut(s)	1683
Hin1I	GRCGYC	1 cut(s)	2334
Hin6I	GCGC	1 cut(s)	1681
HinP1I	GCGC	1 cut(s)	1681
HincII	GTYRAC	1 cut(s)	2293
HindII	GTYRAC	1 cut(s)	2293
HindIII	AAGCTT	3 cut(s)	677, 785, 1095
HinfI	GANTC	8 cut(s)	89, 142, 707, 854, 910, 1613, 2024, 2306
HpaII	CCGG	5 cut(s)	150, 253, 694, 1453, 1873
HphI	GGTGA	5 cut(s)	117, 150, 554, 2074, 2250
Hpy166II	GTNNAC	3 cut(s)	209, 1728, 2293
Hpy188I	TCNGA	7 cut(s)	36, 394, 900, 1501, 1812, 2250, 2348
Hpy8I	GTNNAC	3 cut(s)	209, 1728, 2293
Hpy99I	CGWCG	1 cut(s)	142
HpyAV	CCTTC	8 cut(s)	680, 929, 1618, 1775, 1826, 2132, 2265, 2324
HpyCH4IV	ACGT	1 cut(s)	2180
HpyF10VI	GCNNNNNNNGC	9 cut(s)	332, 631, 845, 1193, 1469, 2148, 2222, 2340, 2349
HpyF3I	CTNAG	7 cut(s)	144, 300, 507, 1500, 1615, 1811, 1911
HpySE526I	ACGT	1 cut(s)	2180
Hsp92I	GRCGYC	1 cut(s)	2334
HspAI	GCGC	1 cut(s)	1681
LmnI	GCTCC	5 cut(s)	69, 1327, 1424, 1432, 1874
Lsp1109I	GCAGC	4 cut(s)	234, 637, 660, 939
MaeI	CTAG	4 cut(s)	314, 923, 1002, 1398
MaeII	ACGT	1 cut(s)	2180
MaeIII	GTNAC	7 cut(s)	445, 542, 596, 1307, 1357, 1583, 2125
MbiI	CCGCTC	1 cut(s)	112
MboII	GAAGA	5 cut(s)	332, 1548, 2044, 2088, 2375
MflI	RGATCY	4 cut(s)	520, 664, 868, 1652
MhlI	GDGCHC	5 cut(s)	301, 930, 1332, 1405, 1429
MlsI	TGGCCA	1 cut(s)	1266
MluNI	TGGCCA	1 cut(s)	1266
MlyI	GAGTC	3 cut(s)	136, 1607, 2315
MmeI	TCCRAC	2 cut(s)	1513, 2001
Mox20I	TGGCCA	1 cut(s)	1266
Mph1103I	ATGCAT	1 cut(s)	270
MscI	TGGCCA	1 cut(s)	1266
MslI	CAYNNNNRTG	3 cut(s)	1179, 1374, 1767
Msp20I	TGGCCA	1 cut(s)	1266
MspA1I	CMGCKG	2 cut(s)	382, 848
MspCI	CTTAAG	2 cut(s)	1345, 1591
MspI	CCGG	5 cut(s)	150, 253, 694, 1453, 1873
MspR9I	CCNGG	6 cut(s)	253, 254, 366, 1454, 1873, 2340
Mva1269I	GAATGC	1 cut(s)	2216
MvaI	CCWGG	2 cut(s)	366, 2340
MwoI	GCNNNNNNNGC	9 cut(s)	332, 631, 845, 1193, 1469, 2148, 2222, 2340, 2349
NciI	CCSGG	4 cut(s)	253, 254, 1454, 1873
NdeI	CATATG	1 cut(s)	557
NlaIV	GGNNCC	5 cut(s)	522, 870, 1087, 1254, 2367
NmeAIII	GCCGAG	1 cut(s)	909
NmuCI	GTSAC	5 cut(s)	445, 542, 1357, 1583, 2125
NsiI	ATGCAT	1 cut(s)	270
NspI	RCATGY	1 cut(s)	1249
PaeR7I	CTCGAG	4 cut(s)	615, 1337, 2111, 2303
PagI	TCATGA	1 cut(s)	2185
PceI	AGGCCT	1 cut(s)	1521
PcsI	WCGNNNNNNNCGW	2 cut(s)	281, 441
PctI	GAATGC	1 cut(s)	2216
PfeI	GAWTC	5 cut(s)	89, 707, 854, 910, 2024
Pfl23II	CGTACG	1 cut(s)	285
PflMI	CCANNNNNTGG	2 cut(s)	497, 1262
PkrI	GCNGC	4 cut(s)	224, 627, 650, 954
PleI	GAGTC	3 cut(s)	136, 1607, 2314
PpsI	GAGTC	3 cut(s)	136, 1607, 2314
PpuMI	RGGWCCY	1 cut(s)	1085
PshBI	ATTAAT	2 cut(s)	1017, 1701
PsiI	TTATAA	1 cut(s)	1604
Psp124BI	GAGCTC	3 cut(s)	930, 1332, 1429
Psp5II	RGGWCCY	1 cut(s)	1085
Psp6I	CCWGG	2 cut(s)	364, 2338
PspEI	GGTNACC	1 cut(s)	596
PspFI	CCCAGC	2 cut(s)	382, 1139
PspGI	CCWGG	2 cut(s)	364, 2338
PspLI	CGTACG	1 cut(s)	285
PspN4I	GGNNCC	5 cut(s)	522, 870, 1087, 1254, 2367
PspPI	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
PspPPI	RGGWCCY	1 cut(s)	1085
PspXI	VCTCGAGB	1 cut(s)	615
PsuI	RGATCY	4 cut(s)	520, 664, 868, 1652
PvuII	CAGCTG	1 cut(s)	848
RsaI	GTAC	5 cut(s)	73, 86, 287, 657, 739
RsaNI	GTAC	5 cut(s)	72, 85, 286, 656, 738
RseI	CAYNNNNRTG	3 cut(s)	1179, 1374, 1767
SacI	GAGCTC	3 cut(s)	930, 1332, 1429
SalI	GTCGAC	1 cut(s)	2291
SatI	GCNGC	4 cut(s)	223, 626, 649, 953
Sau96I	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
SchI	GAGTC	3 cut(s)	136, 1607, 2315
ScrFI	CCNGG	6 cut(s)	253, 254, 366, 1454, 1873, 2340
SduI	GDGCHC	5 cut(s)	301, 930, 1332, 1405, 1429
SfcI	CTRYAG	2 cut(s)	577, 942
Sfr274I	CTCGAG	4 cut(s)	615, 1337, 2111, 2303
SinI	GGWCC	2 cut(s)	1085, 1252
SlaI	CTCGAG	4 cut(s)	615, 1337, 2111, 2303
SmaI	CCCGGG	1 cut(s)	254
SmiMI	CAYNNNNRTG	3 cut(s)	1179, 1374, 1767
SmlI	CTYRAG	6 cut(s)	615, 1337, 1345, 1591, 2111, 2303
SmoI	CTYRAG	6 cut(s)	615, 1337, 1345, 1591, 2111, 2303
SseBI	AGGCCT	1 cut(s)	1521
SsiI	CCGC	4 cut(s)	110, 380, 441, 1442
SspMI	CTAG	4 cut(s)	314, 923, 1002, 1398
SstI	GAGCTC	3 cut(s)	930, 1332, 1429
StuI	AGGCCT	1 cut(s)	1521
StyD4I	CCNGG	6 cut(s)	251, 252, 364, 1452, 1871, 2338
StyI	CCWWGG	1 cut(s)	1505
TaiI	ACGT	1 cut(s)	2183
TatI	WGTACW	1 cut(s)	655
TfiI	GAWTC	5 cut(s)	89, 707, 854, 910, 2024
TscAI	CASTG	4 cut(s)	865, 1358, 1366, 2015
TseFI	GTSAC	5 cut(s)	445, 542, 1357, 1583, 2125
TseI	GCWGC	4 cut(s)	222, 625, 648, 952
Tsp45I	GTSAC	5 cut(s)	445, 542, 1357, 1583, 2125
TspGWI	ACGGA	1 cut(s)	2044
TspMI	CCCGGG	1 cut(s)	252
TspRI	CASTG	4 cut(s)	865, 1358, 1366, 2015
Van91I	CCANNNNNTGG	2 cut(s)	497, 1262
Vha464I	CTTAAG	2 cut(s)	1345, 1591
VpaK11BI	GGWCC	2 cut(s)	1085, 1252
VspI	ATTAAT	2 cut(s)	1017, 1701
XapI	RAATTY	3 cut(s)	104, 965, 1106
XbaI	TCTAGA	1 cut(s)	922
XceI	RCATGY	1 cut(s)	1249
XhoI	CTCGAG	4 cut(s)	615, 1337, 2111, 2303
XmaI	CCCGGG	1 cut(s)	252
XmiI	GTMKAC	1 cut(s)	2292
XspI	CTAG	4 cut(s)	314, 923, 1002, 1398
Zsp2I	ATGCAT	1 cut(s)	270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.