Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0003586

Physical Location & Seq

Genomic Coordinates Forward (+)

6158278 .. 6171334

Gene Structure 13057 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00223020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGAAACTGTGCCATTATCAACATCATCATAATCAGAATAGCAATCGAGAAAGATCAAAGATGCTCCTGCGTACCCCACATTTGTACGATTCAAATGTCTCCAAATTCCCGCTCTTTGCATCATCATCACCATTTTCGACAACTCAGGCCGGTGCTTCTCCGTGTATAGTAGCTTCCCCTGTTCTTGATGTTGGGTTGAAGTTGAGTAAACAAGCAACTGGCAGCAATCAGCAAGGGCTTGTGAGAAAGACCCGGGATCATGTCATGCATAGCACGGGTAATGCGTACGGTCAGGGTGCTCAGGGAAGTGTGCTAGAGGAAGAAGCCCAGCTGGCAGAGACCAGCCAGTCATGGAAAACTTTTCCAGGTCAGGCTTTTCCGCTGGGTGTATCAGAAGTTGATACTGGTGTCAATTTTGCCATTTTCTCACAGCACGCAACCGCTGTCATACTTTGCATTAAACTTGCAGAGAGGGGACAGTTTGGAGGGCCAGATGGTGGAATGATTGAGCTGGCTTTGGATCCTAAAGAGAACAAGACAGGTGACATTTGGCACATATGTATAAAGGATTTTCCCCGTAGCAATGTCCTCTATGGTTACCGTATTGATGGACCTCAAGGTTGGCAGCAAGGGCATCGGTTTGACAGCAGCATTGTCCTAATAGATCCTTATGCGAAGCTAGTTGAAGGCCGCCGGTATTTTGGAGATTCCAGAGAAAAGTTATCTGGGTTTCTTGGTACTTATGATTTTGATAGCTTGCCTTTTGATTGGGGAGACAACTACAAGCTTCCAAATATACCCGAGAATGATCTTGTTATATATGAAATGAATGTTCGTGCATTTACAGCTGATGAATCCAGTGGTCTGGATCCAGATGTTCGTGGTAGCTACCATGGTCTGATTGACAAGATTCCACACCTTCTAGAGCTCGGCATCAATGCTGTAGAGTTGCTGCCTGTCTTTGAATTTGATGAGTTTGAGTTTCAAAGGCGTTCAAATCCTAGAGATCACATGATTAATACATGGGGCTACTCAACGATAAACTTCTTTGCCCCTATGAGCCGTTATGCGAGTGCTGGTGGAGGACCCCTTAAAGCTTCCCATGAATTTAAAGAAATGGTTAAAGCCTTGCATGGTGCAGGGATAGAGGTCATTTTGGATGTTGTCTATAACCATACAAATGAAGCTGATGATGCCTACCCTTATACCACTTCATTTCGTGGCATAGATAATAAGGTTTATTACATGCTGGACCCAAACAATGGCCAATTACTGAACTTCTCGGGCTGTGGGAACTCATTTAACTGTAACCATCCTGTTGTTATGGAACTCATTCTCGAGAGCTTAAGACACTGGGTCACTGAATATCATGTGGATGGATTTAGATTTGACCTTGCTAGTGCTCTTTGTCGGGGAACAGATGGAGCTCCACTTGATGCGCCGCCACTTATCCGGGCTATCGCAAAAGATGCCATTCTATCGAGATGTAAAGTTATTTCTGAGCCTTGGGATTGTGGAGGCCTTTATCTTGTTGGAAGATTTCCAAACTGGGACAGGTGGGCCGAATGGAATGGTATATATCGTGACGACTTGAGGAGATTTATAAAGGGAGACTCAGGTATGAAAGGAAGTTTTGCAACTCGTGTTTCTGGATCTGCTGACCTTTACAAAACGAATAAGCGCAAGCCTTATCACAGTATTAATTTTATAATTGCACATGATGGGTTCACACTATATGATCTCGTTTCATACAATTTCAAGCACAATGATGCTAACGGAGAAGGAGGAAATGATGGAAGTAATGATAATCTGAGCTGGAATTGTGGTTATGAAGGAGAAACCGATGATTCTACTGTTAAAGCTATACGCTCCCGGCAAATGAAAAACTTCCATCTGGCGTTGATGATCTCTCAGGGAACACCAATGATAATGATGGGAGATGAATATGGGCATACTCGATATGGAAATAACAACAGTTATGGACATGATACTGCTATTAACCATTTCCAGTGGGGACTGTTGGAATCACGGAAGAATAATCACTTCAGGTTTTTCAAAGAGGTGATAAACTATCGAAGAACACATCGTGTATTTGGCCGTGATAGATTTCTTGAGAAAAGTGATGTGACATGGCATGAAGGCAACTGGGACAATTATGAGAGCAAGTTTCTTGCATTTACGTTTCATGACAATAATGGAGGAGACATCTACATGGCATTCAATGCTCATGATTATTTTATTGAAGTTCCAATCCCCTCACCACCAGAAAAGAGGATTTGGTTTCGTGTGGTCGACACTAATCTTGAGTCTCCCAATGACTTTGTGCTTGAAGGCGTCCCAGGCACTAGAGGCACTTACAACATGGCCCCTTACTCCTCAATTCTTCTTGAAGCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009657 GO:0009660 GO:0009987 GO:0015980 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0071840 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

799

Amino Acids

90.34

Weight (kDa)

5.9

Isoelectric Point (pI)

33.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	127 - 225	1.7e-10	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	290 - 470	2.1e-11	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	713 - 778	9.4e-12	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09020
fragaria_vesca	FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440
malus_domestica	MD08G1188900.v1.1 MD15G1376800.v1.1
prunus_persica	Prupe.1G523500_v2.0.a1 Prupe.1G523500_v2.0.a1
pyrus_communis	pycom08g16260 pycom15g33660
rosa_chinensis	RchiOBHm_Chr7g0238421
rosa_laevigata	RLG00000000911
rosa_multiflora	Rmu_sc0031277.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223020
rosa_rugosa	Rorug07G0312600
rosa_samantha	Rh7AG467500 Rh7BG438200 Rh7CG485500 Rh7DG454000
rosa_wichuraiana	Rw7G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1604, 1709
AasI	GACNNNNNNGTC	1 cut(s)	346
AccB7I	CCANNNNNTGG	2 cut(s)	497, 1262
AccBSI	CCGCTC	1 cut(s)	112
AccI	GTMKAC	1 cut(s)	2292
AciI	CCGC	5 cut(s)	110, 380, 441, 691, 1442
AclWI	GGATC	7 cut(s)	264, 515, 528, 659, 863, 876, 1660
AcoI	YGGCCR	2 cut(s)	1264, 2095
AcsI	RAATTY	3 cut(s)	104, 965, 1106
AcuI	CTGAAG	1 cut(s)	2029
AcyI	GRCGYC	1 cut(s)	2334
AdeI	CACNNNGTG	1 cut(s)	2087
AfaI	GTAC	4 cut(s)	73, 86, 287, 739
AfiI	CCNNNNNNNGG	2 cut(s)	497, 1262
AflII	CTTAAG	1 cut(s)	1345
AhdI	GACNNNNNGTC	1 cut(s)	1355
AjnI	CCWGG	2 cut(s)	364, 2338
Alw21I	GWGCWC	4 cut(s)	301, 930, 1405, 1429
Alw26I	GTCTC	6 cut(s)	103, 332, 768, 1605, 2196, 2313
AlwI	GGATC	7 cut(s)	264, 515, 528, 659, 863, 876, 1660
Ama87I	CYCGRG	4 cut(s)	252, 800, 1282, 1337
AoxI	GGCC	8 cut(s)	147, 488, 688, 1264, 1519, 1560, 2095, 2364
ApeKI	GCWGC	4 cut(s)	222, 625, 648, 952
ApoI	RAATTY	3 cut(s)	104, 965, 1106
AseI	ATTAAT	2 cut(s)	1017, 1701
Asp700I	GAANNNNTTC	2 cut(s)	1332, 1886
AspLEI	GCGC	2 cut(s)	1441, 1683
AspS9I	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
AsuC2I	CCSGG	4 cut(s)	253, 254, 1454, 1873
AsuHPI	GGTGA	4 cut(s)	120, 554, 2074, 2250
AvaI	CYCGRG	4 cut(s)	252, 800, 1282, 1337
AvaII	GGWCC	3 cut(s)	611, 1085, 1252
BaeI	ACNNNNGTAYC	2 cut(s)	393, 426
BalI	TGGCCA	1 cut(s)	1266
BamHI	GGATCC	2 cut(s)	520, 868
BanII	GRGCYC	2 cut(s)	930, 1429
BarI	GAAGNNNNNNTAC	2 cut(s)	157, 189
BauI	CACGAG	1 cut(s)	1641
Bbv12I	GWGCWC	4 cut(s)	301, 930, 1405, 1429
BbvI	GCAGC	4 cut(s)	234, 637, 660, 939
BccI	CCATC	9 cut(s)	488, 602, 1320, 1370, 1415, 1715, 1787, 1899, 1927
BceAI	ACGGC	2 cut(s)	1047, 2082
BcgI	CGANNNNNNTGC	2 cut(s)	617, 651
BciT130I	CCWGG	2 cut(s)	366, 2340
BcnI	CCSGG	4 cut(s)	253, 254, 1454, 1873
BcoDI	GTCTC	6 cut(s)	103, 332, 768, 1605, 2196, 2313
BfaI	CTAG	6 cut(s)	314, 680, 923, 1002, 1398, 2346
BfmI	CTRYAG	1 cut(s)	942
BfrI	CTTAAG	1 cut(s)	1345
BglI	GCCNNNNNGGC	1 cut(s)	332
BisI	GCNGC	6 cut(s)	223, 626, 649, 691, 953, 1442
BlsI	GCNGC	6 cut(s)	224, 627, 650, 692, 954, 1443
Bme1390I	CCNGG	6 cut(s)	253, 254, 366, 1454, 1873, 2340
Bme18I	GGWCC	3 cut(s)	611, 1085, 1252
BmeRI	GACNNNNNGTC	1 cut(s)	1355
BmeT110I	CYCGRG	4 cut(s)	252, 800, 1282, 1337
BmgT120I	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
BmiI	GGNNCC	5 cut(s)	522, 870, 1087, 1254, 2367
BmrFI	CCNGG	6 cut(s)	253, 254, 366, 1454, 1873, 2340
BmrI	ACTGGG	3 cut(s)	1363, 1558, 2155
BmsI	GCATC	8 cut(s)	51, 128, 643, 942, 1183, 1426, 1459, 1759
BmuI	ACTGGG	3 cut(s)	1363, 1558, 2155
Bpu10I	CCTNAGC	1 cut(s)	300
BpuEI	CTTGAG	4 cut(s)	600, 1612, 2132, 2324
BpuMI	CCSGG	4 cut(s)	253, 254, 1454, 1873
BsaHI	GRCGYC	1 cut(s)	2334
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	4 cut(s)	252, 892, 1505, 2338
Bsc4I	CCNNNNNNNGG	2 cut(s)	497, 1262
Bse118I	RCCGGY	2 cut(s)	149, 693
Bse1I	ACTGG	8 cut(s)	223, 346, 409, 858, 1358, 1553, 2008, 2150
Bse3DI	GCAATG	1 cut(s)	589
BseBI	CCWGG	2 cut(s)	366, 2340
BseDI	CCNNGG	4 cut(s)	252, 892, 1505, 2338
BseGI	GGATG	3 cut(s)	1165, 1312, 1381
BseLI	CCNNNNNNNGG	2 cut(s)	497, 1262
BseMI	GCAATG	1 cut(s)	589
BseMII	CTCAG	6 cut(s)	158, 314, 1491, 1629, 1802, 1925
BseNI	ACTGG	8 cut(s)	223, 346, 409, 858, 1358, 1553, 2008, 2150
BseRI	GAGGAG	3 cut(s)	1609, 2214, 2365
BseXI	GCAGC	4 cut(s)	234, 637, 660, 939
BseYI	CCCAGC	2 cut(s)	327, 382
BsgI	GTGCAG	1 cut(s)	1158
BshFI	GGCC	8 cut(s)	149, 490, 690, 1266, 1521, 1562, 2097, 2366
BsiHKAI	GWGCWC	4 cut(s)	301, 930, 1405, 1429
BsiHKCI	CYCGRG	4 cut(s)	252, 800, 1282, 1337
BsiSI	CCGG	5 cut(s)	150, 253, 694, 1453, 1873
BsiWI	CGTACG	1 cut(s)	285
BslFI	GGGAC	5 cut(s)	489, 1565, 2028, 2162, 2321
BslI	CCNNNNNNNGG	2 cut(s)	497, 1262
BsmAI	GTCTC	6 cut(s)	103, 332, 768, 1605, 2196, 2313
BsmFI	GGGAC	5 cut(s)	489, 1565, 2028, 2162, 2321
BsmI	GAATGC	1 cut(s)	2216
BsnI	GGCC	8 cut(s)	149, 490, 690, 1266, 1521, 1562, 2097, 2366
Bso31I	GGTCTC	1 cut(s)	332
BsoBI	CYCGRG	4 cut(s)	252, 800, 1282, 1337
Bsp1286I	GDGCHC	4 cut(s)	301, 930, 1405, 1429
Bsp19I	CCATGG	1 cut(s)	892
BspACI	CCGC	5 cut(s)	110, 380, 441, 691, 1442
BspANI	GGCC	8 cut(s)	149, 490, 690, 1266, 1521, 1562, 2097, 2366
BspCNI	CTCAG	6 cut(s)	157, 313, 1492, 1628, 1803, 1924
BspHI	TCATGA	2 cut(s)	2185, 2227
BspLI	GGNNCC	5 cut(s)	522, 870, 1087, 1254, 2367
BspPI	GGATC	7 cut(s)	264, 515, 528, 659, 863, 876, 1660
BspTI	CTTAAG	1 cut(s)	1345
BspTNI	GGTCTC	1 cut(s)	332
BsrBI	CCGCTC	1 cut(s)	112
BsrDI	GCAATG	1 cut(s)	589
BsrFI	RCCGGY	2 cut(s)	149, 693
BsrI	ACTGG	8 cut(s)	223, 346, 409, 858, 1358, 1553, 2008, 2150
BssAI	RCCGGY	2 cut(s)	149, 693
BssECI	CCNNGG	4 cut(s)	252, 892, 1505, 2338
BssNI	GRCGYC	1 cut(s)	2334
BssSI	CACGAG	1 cut(s)	1641
BssT1I	CCWWGG	2 cut(s)	892, 1505
Bst2BI	CACGAG	1 cut(s)	1641
Bst2UI	CCWGG	2 cut(s)	366, 2340
Bst4CI	ACNGT	9 cut(s)	9, 290, 480, 602, 1307, 1697, 1855, 1976, 2019
BstACI	GRCGYC	1 cut(s)	2334
BstAFI	CTTAAG	1 cut(s)	1345
BstAPI	GCANNNNNTGC	2 cut(s)	1469, 2222
BstC8I	GCNNGC	5 cut(s)	333, 435, 513, 758, 1685
BstDEI	CTNAG	6 cut(s)	144, 300, 1500, 1615, 1811, 1911
BstDSI	CCRYGG	1 cut(s)	892
BstEII	GGTNACC	1 cut(s)	596
BstF5I	GGATG	3 cut(s)	1165, 1312, 1381
BstHHI	GCGC	2 cut(s)	1441, 1683
BstMAI	GTCTC	6 cut(s)	103, 332, 768, 1605, 2196, 2313
BstMWI	GCNNNNNNNGC	8 cut(s)	332, 631, 845, 1193, 1469, 2222, 2340, 2349
BstNI	CCWGG	2 cut(s)	366, 2340
BstNSI	RCATGY	1 cut(s)	1249
BstPI	GGTNACC	1 cut(s)	596
BstSCI	CCNGG	6 cut(s)	251, 252, 364, 1452, 1871, 2338
BstSFI	CTRYAG	1 cut(s)	942
BstV1I	GCAGC	4 cut(s)	234, 637, 660, 939
BstX2I	RGATCY	4 cut(s)	520, 664, 868, 1652
BstXI	CCANNNNNNTGG	1 cut(s)	865
BstYI	RGATCY	4 cut(s)	520, 664, 868, 1652
BsuRI	GGCC	8 cut(s)	149, 490, 690, 1266, 1521, 1562, 2097, 2366
BtgI	CCRYGG	1 cut(s)	892
BtsCI	GGATG	3 cut(s)	1165, 1312, 1381
BtsIMutI	CAGTG	4 cut(s)	865, 1351, 1359, 2015
Cac8I	GCNNGC	5 cut(s)	333, 435, 513, 758, 1685
CciI	TCATGA	2 cut(s)	2185, 2227
CfoI	GCGC	2 cut(s)	1441, 1683
Cfr10I	RCCGGY	2 cut(s)	149, 693
Cfr13I	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
Cfr9I	CCCGGG	1 cut(s)	252
CseI	GACGC	1 cut(s)	2323
Csp6I	GTAC	4 cut(s)	72, 85, 286, 738
CviQI	GTAC	4 cut(s)	72, 85, 286, 738
DdeI	CTNAG	6 cut(s)	144, 300, 1500, 1615, 1811, 1911
DraI	TTTAAA	1 cut(s)	1111
DraIII	CACNNNGTG	1 cut(s)	2087
DrdI	GACNNNNNNGTC	1 cut(s)	346
DriI	GACNNNNNGTC	1 cut(s)	1355
DseDI	GACNNNNNNGTC	1 cut(s)	346
EaeI	YGGCCR	2 cut(s)	1264, 2095
Eam1105I	GACNNNNNGTC	1 cut(s)	1355
Ecl136II	GAGCTC	2 cut(s)	928, 1427
Eco130I	CCWWGG	2 cut(s)	892, 1505
Eco147I	AGGCCT	1 cut(s)	1521
Eco24I	GRGCYC	2 cut(s)	930, 1429
Eco31I	GGTCTC	1 cut(s)	332
Eco47I	GGWCC	3 cut(s)	611, 1085, 1252
Eco53kI	GAGCTC	2 cut(s)	928, 1427
Eco57I	CTGAAG	1 cut(s)	2029
Eco88I	CYCGRG	4 cut(s)	252, 800, 1282, 1337
Eco91I	GGTNACC	1 cut(s)	596
EcoICRI	GAGCTC	2 cut(s)	928, 1427
EcoO109I	RGGNCCY	1 cut(s)	1085
EcoO65I	GGTNACC	1 cut(s)	596
EcoRII	CCWGG	2 cut(s)	364, 2338
EcoT14I	CCWWGG	2 cut(s)	892, 1505
EcoT22I	ATGCAT	1 cut(s)	270
EcoT38I	GRGCYC	2 cut(s)	930, 1429
ErhI	CCWWGG	2 cut(s)	892, 1505
FaqI	GGGAC	5 cut(s)	489, 1565, 2028, 2162, 2321
FauI	CCCGC	1 cut(s)	117
FauNDI	CATATG	1 cut(s)	557
FblI	GTMKAC	1 cut(s)	2292
Fnu4HI	GCNGC	6 cut(s)	223, 626, 649, 691, 953, 1442
FokI	GGATG	3 cut(s)	1172, 1299, 1388
FriOI	GRGCYC	2 cut(s)	930, 1429
Fsp4HI	GCNGC	6 cut(s)	223, 626, 649, 691, 953, 1442
FspBI	CTAG	6 cut(s)	314, 680, 923, 1002, 1398, 2346
GlaI	GCGC	2 cut(s)	1440, 1682
GluI	GCNGC	6 cut(s)	223, 626, 649, 691, 953, 1442
GsaI	CCCAGC	2 cut(s)	331, 386
HaeIII	GGCC	8 cut(s)	149, 490, 690, 1266, 1521, 1562, 2097, 2366
HapII	CCGG	5 cut(s)	150, 253, 694, 1453, 1873
HgaI	GACGC	1 cut(s)	2323
HhaI	GCGC	2 cut(s)	1441, 1683
Hin1I	GRCGYC	1 cut(s)	2334
Hin6I	GCGC	2 cut(s)	1439, 1681
HinP1I	GCGC	2 cut(s)	1439, 1681
HincII	GTYRAC	1 cut(s)	2293
HindII	GTYRAC	1 cut(s)	2293
HindIII	AAGCTT	2 cut(s)	785, 1095
HinfI	GANTC	8 cut(s)	89, 707, 854, 910, 1613, 1847, 2024, 2306
HpaII	CCGG	5 cut(s)	150, 253, 694, 1453, 1873
HphI	GGTGA	4 cut(s)	120, 554, 2074, 2250
Hpy166II	GTNNAC	3 cut(s)	209, 1728, 2293
Hpy188I	TCNGA	5 cut(s)	36, 394, 900, 1501, 1812
Hpy8I	GTNNAC	3 cut(s)	209, 1728, 2293
HpyAV	CCTTC	6 cut(s)	680, 929, 1775, 1826, 2132, 2324
HpyCH4III	ACNGT	9 cut(s)	9, 290, 480, 602, 1307, 1697, 1855, 1976, 2019
HpyCH4IV	ACGT	1 cut(s)	2180
HpyF10VI	GCNNNNNNNGC	8 cut(s)	332, 631, 845, 1193, 1469, 2222, 2340, 2349
HpyF3I	CTNAG	6 cut(s)	144, 300, 1500, 1615, 1811, 1911
HpySE526I	ACGT	1 cut(s)	2180
Hsp92I	GRCGYC	1 cut(s)	2334
HspAI	GCGC	2 cut(s)	1439, 1681
LmnI	GCTCC	4 cut(s)	69, 1424, 1432, 1874
Lsp1109I	GCAGC	4 cut(s)	234, 637, 660, 939
LweI	GCATC	8 cut(s)	51, 128, 643, 942, 1183, 1426, 1459, 1759
MaeI	CTAG	6 cut(s)	314, 680, 923, 1002, 1398, 2346
MaeII	ACGT	1 cut(s)	2180
MaeIII	GTNAC	6 cut(s)	542, 596, 1307, 1357, 1583, 2125
MbiI	CCGCTC	1 cut(s)	112
MboII	GAAGA	5 cut(s)	332, 1548, 2044, 2088, 2375
MflI	RGATCY	4 cut(s)	520, 664, 868, 1652
MhlI	GDGCHC	4 cut(s)	301, 930, 1405, 1429
MlsI	TGGCCA	1 cut(s)	1266
MluNI	TGGCCA	1 cut(s)	1266
MlyI	GAGTC	2 cut(s)	1607, 2315
MmeI	TCCRAC	2 cut(s)	1513, 2001
Mox20I	TGGCCA	1 cut(s)	1266
Mph1103I	ATGCAT	1 cut(s)	270
MroXI	GAANNNNTTC	2 cut(s)	1332, 1886
MscI	TGGCCA	1 cut(s)	1266
MslI	CAYNNNNRTG	4 cut(s)	1179, 1374, 1767, 2210
Msp20I	TGGCCA	1 cut(s)	1266
MspA1I	CMGCKG	4 cut(s)	331, 382, 443, 848
MspCI	CTTAAG	1 cut(s)	1345
MspI	CCGG	5 cut(s)	150, 253, 694, 1453, 1873
MspR9I	CCNGG	6 cut(s)	253, 254, 366, 1454, 1873, 2340
Mva1269I	GAATGC	1 cut(s)	2216
MvaI	CCWGG	2 cut(s)	366, 2340
MwoI	GCNNNNNNNGC	8 cut(s)	332, 631, 845, 1193, 1469, 2222, 2340, 2349
NciI	CCSGG	4 cut(s)	253, 254, 1454, 1873
NcoI	CCATGG	1 cut(s)	892
NdeI	CATATG	1 cut(s)	557
NlaIV	GGNNCC	5 cut(s)	522, 870, 1087, 1254, 2367
NmeAIII	GCCGAG	1 cut(s)	909
NmuCI	GTSAC	4 cut(s)	542, 1357, 1583, 2125
NsiI	ATGCAT	1 cut(s)	270
NspI	RCATGY	1 cut(s)	1249
PaeR7I	CTCGAG	1 cut(s)	1337
PagI	TCATGA	2 cut(s)	2185, 2227
PceI	AGGCCT	1 cut(s)	1521
PcsI	WCGNNNNNNNCGW	1 cut(s)	281
PctI	GAATGC	1 cut(s)	2216
PdmI	GAANNNNTTC	2 cut(s)	1332, 1886
PfeI	GAWTC	6 cut(s)	89, 707, 854, 910, 1847, 2024
Pfl23II	CGTACG	1 cut(s)	285
PflMI	CCANNNNNTGG	2 cut(s)	497, 1262
PkrI	GCNGC	6 cut(s)	224, 627, 650, 692, 954, 1443
PleI	GAGTC	2 cut(s)	1607, 2314
PpsI	GAGTC	2 cut(s)	1607, 2314
PpuMI	RGGWCCY	1 cut(s)	1085
PshBI	ATTAAT	2 cut(s)	1017, 1701
PsiI	TTATAA	2 cut(s)	1604, 1709
Psp124BI	GAGCTC	2 cut(s)	930, 1429
Psp5II	RGGWCCY	1 cut(s)	1085
Psp6I	CCWGG	2 cut(s)	364, 2338
PspEI	GGTNACC	1 cut(s)	596
PspFI	CCCAGC	2 cut(s)	327, 382
PspGI	CCWGG	2 cut(s)	364, 2338
PspLI	CGTACG	1 cut(s)	285
PspN4I	GGNNCC	5 cut(s)	522, 870, 1087, 1254, 2367
PspPI	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
PspPPI	RGGWCCY	1 cut(s)	1085
PsuI	RGATCY	4 cut(s)	520, 664, 868, 1652
PvuII	CAGCTG	2 cut(s)	331, 848
RsaI	GTAC	4 cut(s)	73, 86, 287, 739
RsaNI	GTAC	4 cut(s)	72, 85, 286, 738
RseI	CAYNNNNRTG	4 cut(s)	1179, 1374, 1767, 2210
SacI	GAGCTC	2 cut(s)	930, 1429
SalI	GTCGAC	1 cut(s)	2291
SatI	GCNGC	6 cut(s)	223, 626, 649, 691, 953, 1442
Sau96I	GGNCC	6 cut(s)	488, 611, 1085, 1252, 1560, 2365
SchI	GAGTC	2 cut(s)	1607, 2315
ScrFI	CCNGG	6 cut(s)	253, 254, 366, 1454, 1873, 2340
SduI	GDGCHC	4 cut(s)	301, 930, 1405, 1429
SfaNI	GCATC	8 cut(s)	51, 128, 643, 942, 1183, 1426, 1459, 1759
SfcI	CTRYAG	1 cut(s)	942
Sfr274I	CTCGAG	1 cut(s)	1337
SinI	GGWCC	3 cut(s)	611, 1085, 1252
SlaI	CTCGAG	1 cut(s)	1337
SmaI	CCCGGG	1 cut(s)	254
SmiMI	CAYNNNNRTG	4 cut(s)	1179, 1374, 1767, 2210
SmlI	CTYRAG	6 cut(s)	615, 1337, 1345, 1591, 2111, 2303
SmoI	CTYRAG	6 cut(s)	615, 1337, 1345, 1591, 2111, 2303
SseBI	AGGCCT	1 cut(s)	1521
SsiI	CCGC	5 cut(s)	110, 380, 441, 691, 1442
SspMI	CTAG	6 cut(s)	314, 680, 923, 1002, 1398, 2346
SstI	GAGCTC	2 cut(s)	930, 1429
StuI	AGGCCT	1 cut(s)	1521
StyD4I	CCNGG	6 cut(s)	251, 252, 364, 1452, 1871, 2338
StyI	CCWWGG	2 cut(s)	892, 1505
TaaI	ACNGT	9 cut(s)	9, 290, 480, 602, 1307, 1697, 1855, 1976, 2019
TaiI	ACGT	1 cut(s)	2183
TaqI	TCGA	7 cut(s)	46, 137, 1338, 1481, 1957, 2074, 2292
TauI	GCSGC	2 cut(s)	693, 1444
TfiI	GAWTC	6 cut(s)	89, 707, 854, 910, 1847, 2024
TscAI	CASTG	4 cut(s)	865, 1358, 1366, 2015
TseFI	GTSAC	4 cut(s)	542, 1357, 1583, 2125
TseI	GCWGC	4 cut(s)	222, 625, 648, 952
Tsp45I	GTSAC	4 cut(s)	542, 1357, 1583, 2125
TspGWI	ACGGA	3 cut(s)	150, 1791, 2044
TspMI	CCCGGG	1 cut(s)	252
TspRI	CASTG	4 cut(s)	865, 1358, 1366, 2015
Van91I	CCANNNNNTGG	2 cut(s)	497, 1262
Vha464I	CTTAAG	1 cut(s)	1345
VpaK11BI	GGWCC	3 cut(s)	611, 1085, 1252
VspI	ATTAAT	2 cut(s)	1017, 1701
XapI	RAATTY	3 cut(s)	104, 965, 1106
XbaI	TCTAGA	1 cut(s)	922
XceI	RCATGY	1 cut(s)	1249
XhoI	CTCGAG	1 cut(s)	1337
XmaI	CCCGGG	1 cut(s)	252
XmiI	GTMKAC	1 cut(s)	2292
XmnI	GAANNNNTTC	2 cut(s)	1332, 1886
XspI	CTAG	6 cut(s)	314, 680, 923, 1002, 1398, 2346
Zsp2I	ATGCAT	1 cut(s)	270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.