Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003586

Physical Location & Seq

Genomic Coordinates Forward (+)

23867901 .. 23881201

Gene Structure 13301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1188900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGATGCTTCGAACCCCACATTTGTACGATTCAAATACCTCCATGTTCCCTCTCTTGGCATCTCCCCCTTCATCTCACAGTAGTGCTTCATGCACAGTGGCTTCTCCTGTCATCGAAATGGGATTGAAGTGGAGTAAACATGGATCGGGTAGCAATCCGCAAGGAGTTGTCAGAAAGACCAGGAATGGGGCCAGGCATAGTACAGCCAGTGCTTACGGTCAGCGTGCTCAAGAACGTGTACTTGAGGAAGAAGCCACCCAACTGTCAGAATCGAGCCCATCATGGAAACTTTTCCCAGGTCAAGCTTTTCCGTTGGGTGTATCAGAAGTTGACAGTGGGATAAATTTTGCTATCTTTTCACAGCATGCAACTGCTGTCACACTATGCTTATCATTTCCAGAGAGGCTTGGAGGGCTGGATGGTGGAATGATGGACCTGCCTTTGGATCGTAATGTTAACAAGACCGGTGACATTTGGCACATATGTATCCAGGATTTGCCCAGGAGCAATGTTCTTTATGGTTATCGTATGGATGGGCCACGAGGTTGGCATCAGGGACATCGATTTGACAGCAGCATTGTGCTTGTGGATCCTTACACAAAGCTAGTTGAAGGCCGTCGAATTTTTGGAGATACCACAAAAAAGTTATCTAAATTTCTTGGAACTTATGATTTTGATAGCTTGCCCTTTAACTGGGGAGACAACTACAAGCTCCCAAATATACCTGAGAAGGATCTTGTTATCTACGAAATGAATGTTCGTGCATATACAGCTGATGAATCCAGTGGTTTGGATCCTGATATACGTGGTAGCTACCTTGGTGTGATCCAGAAGATTCCACACCTTCGAGAGCTTGGTATCAATGCAGTGGAACTGCTGCCGGTTGTCGAATTTGATGAGTTTGAGTTCCAGAGGAGACGAAATCCTAGAGATCACATGATAAACACATGGGGCTACTCGACAATAAATTTCTTTGCTCCTATGAGCCGCTATGCAAGTGCTGGTGGAGGACCCCTTAAAGCTTCTCAGGAATTTAAACAAATGGTTAAAGCCTTGCATGGTGCTGGGATAGAGGTCATTTTGGATGTTGTCTATAATCATACTAATGAAGCTGATGATGCCAACCCCTATACAACTTCATTTCGCGGCATAGATAATAAGGTTTACTACATGCTGGACGTAAACAACAATGGCCAATTACTTAACTACTCTGGCTGCGGGAACACATTAAACTGTAACCATCCTGTTGTCATGGAGCTCATTCTTGACAGTTTAAGACATTGGGTTACTGAATATCATGTGGATGGATTTAGATTTGACCTTGCCAGTGTTCTTTGTCGGGGAGCAGATGGGACTCCTCTTGACGCACCACCACTTATTAGGGCTATTGCAAAAGATGCCATCTTATCAAGATGTAAAATCATTTCCGAACCTTGGGATTGTGGAGGCCTTTATCTTGTTGGAAGATTTCCAAATTGGGACAGGTGGGCTGAATGGAATGGGAAATATCGTGATGACTTAAGAAGATTTATAAAGGGTGACTCTGGTATGAAAGGAAGTTTTGCCACTCGCGTTTCTGGATCTGCTGACCTTTACAAAGTGAATAAGCGCAAACCCTATCACAGTGTTAATTTTATTATTGCGCATGATGGGTTCACACTGTATGATCTTGTTTCATACAATTTCAAGCACAATGATGCTAATGGAGAAGGAGGAAACGATGGAAACAATGATAATTTGAGCTGGAATTGTGGTCATGAAGGAGAGACCAATGATTCTACCATTAAAGCTATTCGCTCCCGCCAAATGAAAAACTTTCATTTGGCATTAATGATATCCCAGGGAACCCCAATGATGCTGATGGGGGATGAATACGGGCATACTCGACATGGAAATAACAACAGTTATGGACATGATACTGCTCTTAACCATTTCCAGTGGGGACTGTTGGAGGCACGGAAGAACAATCACTTCAGGTTTTTCAAGGAGGCGATAAAGTTCCGACAAGCACATCGCGTATTTGGTCGTGATACATTTCTTGAGAAATATGATGTGACATGGCATGAAGACAACTGGTACAACTATGAGAGCAAATTCCTTGCATTTACGTTTCATGACAATAGTGAAGGTGACATCTATTTGGCATTCAATGCTCATGACTGTTTTATTACAGTTCCAATGCCCGCACCACCGGTCAAGAGGAAATGGTTTCGAGTGGTTGACACTAATTTGGTGTCTCCCAATGACATCGTGCCTGGTGGCGTCCCAGGCATTGCAGGCACTTACAACATGGCCCCCTTCTCCTCAATTCTTCTTCAAGCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009657 GO:0009660 GO:0009987 GO:0015980 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0071840 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

776

Amino Acids

87.41

Weight (kDa)

6.49

Isoelectric Point (pI)

34.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	104 - 199	3.9e-13	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	265 - 382	5.8e-09	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	692 - 751	6e-11	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09020
fragaria_vesca	FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440
malus_domestica	MD08G1188900.v1.1 MD15G1376800.v1.1
prunus_persica	Prupe.1G523500_v2.0.a1 Prupe.1G523500_v2.0.a1
pyrus_communis	pycom08g16260 pycom15g33660
rosa_chinensis	RchiOBHm_Chr7g0238421
rosa_laevigata	RLG00000000911
rosa_multiflora	Rmu_sc0031277.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223020
rosa_rugosa	Rorug07G0312600
rosa_samantha	Rh7AG467500 Rh7BG438200 Rh7CG485500 Rh7DG454000
rosa_wichuraiana	Rw7G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1532
Acc16I	TGCGCA	1 cut(s)	1644
Acc36I	ACCTGC	1 cut(s)	444
AccII	CGCG	3 cut(s)	1146, 1572, 2016
AciI	CCGC	6 cut(s)	158, 988, 1146, 1218, 1801, 2184
AclWI	GGATC	9 cut(s)	151, 453, 584, 597, 741, 788, 801, 820, 1588
AcoI	YGGCCR	1 cut(s)	1192
AcsI	RAATTY	7 cut(s)	343, 621, 653, 890, 967, 1031, 2093
AcuI	CTGAAG	1 cut(s)	1957
AcyI	GRCGYC	1 cut(s)	2262
AfaI	GTAC	4 cut(s)	26, 202, 240, 2078
AfiI	CCNNNNNNNGG	5 cut(s)	55, 186, 442, 693, 1434
AflII	CTTAAG	1 cut(s)	1519
AflIII	ACRYGT	1 cut(s)	235
AgeI	ACCGGT	2 cut(s)	464, 2191
AgsI	TTSAA	7 cut(s)	33, 127, 611, 1687, 1984, 2149, 2318
AjnI	CCWGG	8 cut(s)	179, 191, 295, 489, 500, 1839, 2254, 2266
AleI	CACNNNNGTG	1 cut(s)	81
Alw21I	GWGCWC	2 cut(s)	229, 1260
Alw26I	GTCTC	4 cut(s)	693, 910, 1760, 2241
AlwI	GGATC	9 cut(s)	151, 453, 584, 597, 741, 788, 801, 820, 1588
AoxI	GGCC	6 cut(s)	189, 536, 613, 1192, 1447, 2292
ApeKI	GCWGC	3 cut(s)	573, 877, 1215
ApoI	RAATTY	7 cut(s)	343, 621, 653, 890, 967, 1031, 2093
AseI	ATTAAT	1 cut(s)	1829
AsiGI	ACCGGT	2 cut(s)	464, 2191
Asp700I	GAANNNNTTC	1 cut(s)	290
AspLEI	GCGC	2 cut(s)	1611, 1645
AspS9I	GGNCC	5 cut(s)	189, 433, 536, 1010, 2293
AsuHPI	GGTGA	3 cut(s)	479, 1550, 2141
AsuII	TTCGAA	1 cut(s)	10
AvaII	GGWCC	2 cut(s)	433, 1010
BaeI	ACNNNNGTAYC	2 cut(s)	469, 502
BalI	TGGCCA	1 cut(s)	1194
BamHI	GGATCC	2 cut(s)	589, 793
BanII	GRGCYC	2 cut(s)	278, 1260
BauI	CACGAG	1 cut(s)	540
BbsI	GAAGAC	1 cut(s)	2073
Bbv12I	GWGCWC	2 cut(s)	229, 1260
BbvI	GCAGC	3 cut(s)	585, 864, 1202
BceAI	ACGGC	1 cut(s)	600
BciT130I	CCWGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
BciVI	GTATCC	1 cut(s)	497
BcoDI	GTCTC	4 cut(s)	693, 910, 1760, 2241
BfaI	CTAG	2 cut(s)	605, 927
BfrI	CTTAAG	1 cut(s)	1519
BfuAI	ACCTGC	1 cut(s)	444
BfuI	GTATCC	1 cut(s)	497
BisI	GCNGC	5 cut(s)	574, 878, 988, 1147, 1216
BlsI	GCNGC	5 cut(s)	575, 879, 989, 1148, 1217
Bme1390I	CCNGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
Bme18I	GGWCC	2 cut(s)	433, 1010
BmgT120I	GGNCC	5 cut(s)	189, 433, 536, 1010, 2293
BmiI	GGNNCC	6 cut(s)	190, 591, 795, 1012, 1846, 2295
BmrFI	CCNGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
BmrI	ACTGGG	1 cut(s)	703
BmsI	GCATC	6 cut(s)	68, 559, 1108, 1387, 1687, 1845
BmuI	ACTGGG	1 cut(s)	703
BpiI	GAAGAC	1 cut(s)	2073
Bpu14I	TTCGAA	1 cut(s)	10
BpuEI	CTTGAG	3 cut(s)	213, 263, 2060
Bsa29I	ATCGAT	1 cut(s)	562
BsaAI	YACGTR	1 cut(s)	806
BsaHI	GRCGYC	1 cut(s)	2262
BsaI	GGTCTC	1 cut(s)	1760
BsaJI	CCNNGG	7 cut(s)	295, 500, 817, 1433, 1839, 1840, 2266
BsaWI	WCCGGW	2 cut(s)	464, 2191
Bsc4I	CCNNNNNNNGG	5 cut(s)	55, 186, 442, 693, 1434
Bse118I	RCCGGY	3 cut(s)	464, 880, 2191
Bse1I	ACTGG	6 cut(s)	207, 698, 783, 1326, 1936, 2078
Bse3DI	GCAATG	2 cut(s)	514, 2271
BseBI	CCWGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
BseCI	ATCGAT	1 cut(s)	562
BseDI	CCNNGG	7 cut(s)	295, 500, 817, 1433, 1839, 1840, 2266
BseGI	GGATG	6 cut(s)	424, 538, 1090, 1240, 1309, 1873
BseLI	CCNNNNNNNGG	5 cut(s)	55, 186, 442, 693, 1434
BseMI	GCAATG	2 cut(s)	514, 2271
BseMII	CTCAG	2 cut(s)	717, 1040
BseNI	ACTGG	6 cut(s)	207, 698, 783, 1326, 1936, 2078
BseRI	GAGGAG	3 cut(s)	928, 1347, 2293
BseXI	GCAGC	3 cut(s)	585, 864, 1202
BseYI	CCCAGC	1 cut(s)	1064
Bsh1236I	CGCG	3 cut(s)	1146, 1572, 2016
BshFI	GGCC	6 cut(s)	191, 538, 615, 1194, 1449, 2294
BshTI	ACCGGT	2 cut(s)	464, 2191
BshVI	ATCGAT	1 cut(s)	562
BsiHKAI	GWGCWC	2 cut(s)	229, 1260
BsiSI	CCGG	3 cut(s)	465, 881, 2192
BslFI	GGGAC	5 cut(s)	570, 1366, 1493, 1956, 2249
BslI	CCNNNNNNNGG	5 cut(s)	55, 186, 442, 693, 1434
BsmAI	GTCTC	4 cut(s)	693, 910, 1760, 2241
BsmBI	CGTCTC	1 cut(s)	910
BsmFI	GGGAC	5 cut(s)	570, 1366, 1493, 1956, 2249
BsmI	GAATGC	1 cut(s)	2144
BsnI	GGCC	6 cut(s)	191, 538, 615, 1194, 1449, 2294
Bso31I	GGTCTC	1 cut(s)	1760
Bsp119I	TTCGAA	1 cut(s)	10
Bsp1286I	GDGCHC	3 cut(s)	229, 278, 1260
Bsp143I	GATC	9 cut(s)	143, 445, 589, 733, 793, 825, 931, 1580, 1666
BspACI	CCGC	6 cut(s)	158, 988, 1146, 1218, 1801, 2184
BspANI	GGCC	6 cut(s)	191, 538, 615, 1194, 1449, 2294
BspCNI	CTCAG	2 cut(s)	718, 1039
BspDI	ATCGAT	1 cut(s)	562
BspFNI	CGCG	3 cut(s)	1146, 1572, 2016
BspHI	TCATGA	3 cut(s)	1756, 2113, 2155
BspLI	GGNNCC	6 cut(s)	190, 591, 795, 1012, 1846, 2295
BspMI	ACCTGC	1 cut(s)	444
BspPI	GGATC	9 cut(s)	151, 453, 584, 597, 741, 788, 801, 820, 1588
BspT104I	TTCGAA	1 cut(s)	10
BspTI	CTTAAG	1 cut(s)	1519
BspTNI	GGTCTC	1 cut(s)	1760
BsrDI	GCAATG	2 cut(s)	514, 2271
BsrFI	RCCGGY	3 cut(s)	464, 880, 2191
BsrI	ACTGG	6 cut(s)	207, 698, 783, 1326, 1936, 2078
BssAI	RCCGGY	3 cut(s)	464, 880, 2191
BssECI	CCNNGG	7 cut(s)	295, 500, 817, 1433, 1839, 1840, 2266
BssMI	GATC	9 cut(s)	143, 445, 589, 733, 793, 825, 931, 1580, 1666
BssNI	GRCGYC	1 cut(s)	2262
BssSI	CACGAG	1 cut(s)	540
BssT1I	CCWWGG	2 cut(s)	817, 1433
Bst2BI	CACGAG	1 cut(s)	540
Bst2UI	CCWGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
BstACI	GRCGYC	1 cut(s)	2262
BstAFI	CTTAAG	1 cut(s)	1519
BstAPI	GCANNNNNTGC	2 cut(s)	1397, 2150
BstBAI	YACGTR	1 cut(s)	806
BstBI	TTCGAA	1 cut(s)	10
BstC8I	GCNNGC	5 cut(s)	225, 366, 683, 2184, 2278
BstDEI	CTNAG	2 cut(s)	726, 1026
BstF5I	GGATG	6 cut(s)	424, 538, 1090, 1240, 1309, 1873
BstFNI	CGCG	3 cut(s)	1146, 1572, 2016
BstHHI	GCGC	2 cut(s)	1611, 1645
BstKTI	GATC	9 cut(s)	146, 448, 592, 736, 796, 828, 934, 1583, 1669
BstMAI	GTCTC	4 cut(s)	693, 910, 1760, 2241
BstMBI	GATC	9 cut(s)	143, 445, 589, 733, 793, 825, 931, 1580, 1666
BstMWI	GCNNNNNNNGC	7 cut(s)	412, 770, 1118, 1397, 2150, 2268, 2277
BstNI	CCWGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
BstNSI	RCATGY	2 cut(s)	368, 1174
BstSCI	CCNGG	8 cut(s)	179, 191, 295, 489, 500, 1839, 2254, 2266
BstUI	CGCG	3 cut(s)	1146, 1572, 2016
BstV1I	GCAGC	3 cut(s)	585, 864, 1202
BstV2I	GAAGAC	1 cut(s)	2073
BstX2I	RGATCY	4 cut(s)	589, 733, 793, 1580
BstXI	CCANNNNNNTGG	2 cut(s)	546, 790
BstYI	RGATCY	4 cut(s)	589, 733, 793, 1580
Bsu15I	ATCGAT	1 cut(s)	562
BsuI	GTATCC	1 cut(s)	497
BsuRI	GGCC	6 cut(s)	191, 538, 615, 1194, 1449, 2294
BsuTUI	ATCGAT	1 cut(s)	562
BtgZI	GCGATG	1 cut(s)	1997
BtsCI	GGATG	6 cut(s)	424, 538, 1090, 1240, 1309, 1873
BtsI	GCAGTG	1 cut(s)	873
BtsIMutI	CAGTG	9 cut(s)	102, 214, 340, 790, 873, 1333, 1630, 1658, 1943
BveI	ACCTGC	1 cut(s)	444
Cac8I	GCNNGC	5 cut(s)	225, 366, 683, 2184, 2278
CciI	TCATGA	3 cut(s)	1756, 2113, 2155
CfoI	GCGC	2 cut(s)	1611, 1645
Cfr10I	RCCGGY	3 cut(s)	464, 880, 2191
Cfr13I	GGNCC	5 cut(s)	189, 433, 536, 1010, 2293
ClaI	ATCGAT	1 cut(s)	562
CseI	GACGC	2 cut(s)	1373, 2251
Csp6I	GTAC	4 cut(s)	25, 201, 239, 2077
CspAI	ACCGGT	2 cut(s)	464, 2191
CviQI	GTAC	4 cut(s)	25, 201, 239, 2077
DdeI	CTNAG	2 cut(s)	726, 1026
DpnI	GATC	9 cut(s)	145, 447, 591, 735, 795, 827, 933, 1582, 1668
DpnII	GATC	9 cut(s)	143, 445, 589, 733, 793, 825, 931, 1580, 1666
DraI	TTTAAA	1 cut(s)	1036
EaeI	YGGCCR	1 cut(s)	1192
Ecl136II	GAGCTC	1 cut(s)	1258
Eco130I	CCWWGG	2 cut(s)	817, 1433
Eco147I	AGGCCT	1 cut(s)	1449
Eco24I	GRGCYC	2 cut(s)	278, 1260
Eco31I	GGTCTC	1 cut(s)	1760
Eco32I	GATATC	1 cut(s)	1836
Eco47I	GGWCC	2 cut(s)	433, 1010
Eco53kI	GAGCTC	1 cut(s)	1258
Eco57I	CTGAAG	1 cut(s)	1957
EcoICRI	GAGCTC	1 cut(s)	1258
EcoO109I	RGGNCCY	1 cut(s)	1010
EcoRII	CCWGG	8 cut(s)	179, 191, 295, 489, 500, 1839, 2254, 2266
EcoRV	GATATC	1 cut(s)	1836
EcoT14I	CCWWGG	2 cut(s)	817, 1433
EcoT38I	GRGCYC	2 cut(s)	278, 1260
ErhI	CCWWGG	2 cut(s)	817, 1433
Esp3I	CGTCTC	1 cut(s)	910
FaqI	GGGAC	5 cut(s)	570, 1366, 1493, 1956, 2249
FauI	CCCGC	3 cut(s)	1211, 1808, 2191
FauNDI	CATATG	1 cut(s)	482
Fnu4HI	GCNGC	5 cut(s)	574, 878, 988, 1147, 1216
FokI	GGATG	6 cut(s)	431, 545, 1097, 1227, 1316, 1880
FriOI	GRGCYC	2 cut(s)	278, 1260
Fsp4HI	GCNGC	5 cut(s)	574, 878, 988, 1147, 1216
FspBI	CTAG	2 cut(s)	605, 927
FspI	TGCGCA	1 cut(s)	1644
GlaI	GCGC	2 cut(s)	1610, 1644
GluI	GCNGC	5 cut(s)	574, 878, 988, 1147, 1216
GsaI	CCCAGC	1 cut(s)	1068
HaeIII	GGCC	6 cut(s)	191, 538, 615, 1194, 1449, 2294
HapII	CCGG	3 cut(s)	465, 881, 2192
HgaI	GACGC	2 cut(s)	1373, 2251
HhaI	GCGC	2 cut(s)	1611, 1645
Hin1I	GRCGYC	1 cut(s)	2262
Hin6I	GCGC	2 cut(s)	1609, 1643
HinP1I	GCGC	2 cut(s)	1609, 1643
HincII	GTYRAC	3 cut(s)	331, 457, 2221
HindII	GTYRAC	3 cut(s)	331, 457, 2221
HindIII	AAGCTT	2 cut(s)	303, 1020
HinfI	GANTC	7 cut(s)	29, 269, 779, 835, 1354, 1541, 1775
HpaI	GTTAAC	1 cut(s)	457
HpaII	CCGG	3 cut(s)	465, 881, 2192
HphI	GGTGA	3 cut(s)	479, 1550, 2141
Hpy166II	GTNNAC	8 cut(s)	137, 239, 331, 457, 1165, 1183, 1656, 2221
Hpy188I	TCNGA	5 cut(s)	173, 268, 325, 1429, 2003
Hpy8I	GTNNAC	8 cut(s)	137, 239, 331, 457, 1165, 1183, 1656, 2221
Hpy99I	CGWCG	1 cut(s)	621
HpyAV	CCTTC	8 cut(s)	78, 605, 724, 854, 1703, 1754, 2120, 2308
HpyCH4IV	ACGT	4 cut(s)	235, 805, 1179, 2108
HpyCH4V	TGCA	9 cut(s)	93, 368, 764, 866, 995, 1057, 1391, 2102, 2276
HpyF10VI	GCNNNNNNNGC	7 cut(s)	412, 770, 1118, 1397, 2150, 2268, 2277
HpyF3I	CTNAG	2 cut(s)	726, 1026
HpySE526I	ACGT	4 cut(s)	235, 805, 1179, 2108
Hsp92I	GRCGYC	1 cut(s)	2262
HspAI	GCGC	2 cut(s)	1609, 1643
KspAI	GTTAAC	1 cut(s)	457
Kzo9I	GATC	9 cut(s)	143, 445, 589, 733, 793, 825, 931, 1580, 1666
LmnI	GCTCC	6 cut(s)	504, 717, 982, 1255, 1343, 1802
Lsp1109I	GCAGC	3 cut(s)	585, 864, 1202
LweI	GCATC	6 cut(s)	68, 559, 1108, 1387, 1687, 1845
MaeI	CTAG	2 cut(s)	605, 927
MaeII	ACGT	4 cut(s)	235, 805, 1179, 2108
MaeIII	GTNAC	7 cut(s)	376, 467, 1235, 1285, 1538, 2053, 2129
MalI	GATC	9 cut(s)	145, 447, 591, 735, 795, 827, 933, 1582, 1668
MboI	GATC	9 cut(s)	143, 445, 589, 733, 793, 825, 931, 1580, 1666
MboII	GAAGA	8 cut(s)	260, 844, 1476, 1536, 1972, 2078, 2303, 2306
MflI	RGATCY	4 cut(s)	589, 733, 793, 1580
MhlI	GDGCHC	3 cut(s)	229, 278, 1260
MlsI	TGGCCA	1 cut(s)	1194
MluNI	TGGCCA	1 cut(s)	1194
MlyI	GAGTC	2 cut(s)	1348, 1535
MmeI	TCCRAC	3 cut(s)	1441, 1929, 2026
Mox20I	TGGCCA	1 cut(s)	1194
MroXI	GAANNNNTTC	1 cut(s)	290
MscI	TGGCCA	1 cut(s)	1194
MslI	CAYNNNNRTG	5 cut(s)	81, 116, 1104, 1302, 1695
Msp20I	TGGCCA	1 cut(s)	1194
MspA1I	CMGCKG	1 cut(s)	773
MspCI	CTTAAG	1 cut(s)	1519
MspI	CCGG	3 cut(s)	465, 881, 2192
MspR9I	CCNGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
Mva1269I	GAATGC	1 cut(s)	2144
MvaI	CCWGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
MvnI	CGCG	3 cut(s)	1146, 1572, 2016
MwoI	GCNNNNNNNGC	7 cut(s)	412, 770, 1118, 1397, 2150, 2268, 2277
NdeI	CATATG	1 cut(s)	482
NdeII	GATC	9 cut(s)	143, 445, 589, 733, 793, 825, 931, 1580, 1666
NlaIV	GGNNCC	6 cut(s)	190, 591, 795, 1012, 1846, 2295
NmuCI	GTSAC	5 cut(s)	376, 467, 1538, 2053, 2129
NsbI	TGCGCA	1 cut(s)	1644
NspI	RCATGY	2 cut(s)	368, 1174
NspV	TTCGAA	1 cut(s)	10
OliI	CACNNNNGTG	1 cut(s)	81
PaeI	GCATGC	1 cut(s)	368
PagI	TCATGA	3 cut(s)	1756, 2113, 2155
PasI	CCCWGGG	1 cut(s)	1840
PceI	AGGCCT	1 cut(s)	1449
PctI	GAATGC	1 cut(s)	2144
PdmI	GAANNNNTTC	1 cut(s)	290
PfeI	GAWTC	5 cut(s)	29, 269, 779, 835, 1775
PfoI	TCCNGGA	1 cut(s)	489
PinAI	ACCGGT	2 cut(s)	464, 2191
PkrI	GCNGC	5 cut(s)	575, 879, 989, 1148, 1217
PleI	GAGTC	2 cut(s)	1348, 1535
PpsI	GAGTC	2 cut(s)	1348, 1535
Ppu21I	YACGTR	1 cut(s)	806
PpuMI	RGGWCCY	1 cut(s)	1010
PshBI	ATTAAT	1 cut(s)	1829
PsiI	TTATAA	1 cut(s)	1532
Psp124BI	GAGCTC	1 cut(s)	1260
Psp5II	RGGWCCY	1 cut(s)	1010
Psp6I	CCWGG	8 cut(s)	179, 191, 295, 489, 500, 1839, 2254, 2266
PspFI	CCCAGC	1 cut(s)	1064
PspGI	CCWGG	8 cut(s)	179, 191, 295, 489, 500, 1839, 2254, 2266
PspN4I	GGNNCC	6 cut(s)	190, 591, 795, 1012, 1846, 2295
PspPI	GGNCC	5 cut(s)	189, 433, 536, 1010, 2293
PspPPI	RGGWCCY	1 cut(s)	1010
PsuI	RGATCY	4 cut(s)	589, 733, 793, 1580
PvuII	CAGCTG	1 cut(s)	773
RsaI	GTAC	4 cut(s)	26, 202, 240, 2078
RsaNI	GTAC	4 cut(s)	25, 201, 239, 2077
RseI	CAYNNNNRTG	5 cut(s)	81, 116, 1104, 1302, 1695
SacI	GAGCTC	1 cut(s)	1260
SatI	GCNGC	5 cut(s)	574, 878, 988, 1147, 1216
Sau3AI	GATC	9 cut(s)	143, 445, 589, 733, 793, 825, 931, 1580, 1666
Sau96I	GGNCC	5 cut(s)	189, 433, 536, 1010, 2293
SchI	GAGTC	2 cut(s)	1348, 1535
ScrFI	CCNGG	8 cut(s)	181, 193, 297, 491, 502, 1841, 2256, 2268
SduI	GDGCHC	3 cut(s)	229, 278, 1260
SfaNI	GCATC	6 cut(s)	68, 559, 1108, 1387, 1687, 1845
SfuI	TTCGAA	1 cut(s)	10
SinI	GGWCC	2 cut(s)	433, 1010
SmiMI	CAYNNNNRTG	5 cut(s)	81, 116, 1104, 1302, 1695
SmlI	CTYRAG	4 cut(s)	228, 242, 1519, 2039
SmoI	CTYRAG	4 cut(s)	228, 242, 1519, 2039
SphI	GCATGC	1 cut(s)	368
SseBI	AGGCCT	1 cut(s)	1449
SsiI	CCGC	6 cut(s)	158, 988, 1146, 1218, 1801, 2184
SspMI	CTAG	2 cut(s)	605, 927
SstI	GAGCTC	1 cut(s)	1260
StuI	AGGCCT	1 cut(s)	1449
StyD4I	CCNGG	8 cut(s)	179, 191, 295, 489, 500, 1839, 2254, 2266
StyI	CCWWGG	2 cut(s)	817, 1433
TaiI	ACGT	4 cut(s)	238, 808, 1182, 2111
TatI	WGTACW	2 cut(s)	200, 238
TauI	GCSGC	2 cut(s)	990, 1149
TfiI	GAWTC	5 cut(s)	29, 269, 779, 835, 1775
TscAI	CASTG	9 cut(s)	102, 214, 340, 790, 873, 1333, 1630, 1665, 1943
TseFI	GTSAC	5 cut(s)	376, 467, 1538, 2053, 2129
TseI	GCWGC	3 cut(s)	573, 877, 1215
Tsp45I	GTSAC	5 cut(s)	376, 467, 1538, 2053, 2129
TspGWI	ACGGA	2 cut(s)	300, 1972
TspRI	CASTG	9 cut(s)	102, 214, 340, 790, 873, 1333, 1630, 1665, 1943
Vha464I	CTTAAG	1 cut(s)	1519
VpaK11BI	GGWCC	2 cut(s)	433, 1010
VspI	ATTAAT	1 cut(s)	1829
XapI	RAATTY	7 cut(s)	343, 621, 653, 890, 967, 1031, 2093
XceI	RCATGY	2 cut(s)	368, 1174
XmnI	GAANNNNTTC	1 cut(s)	290
XspI	CTAG	2 cut(s)	605, 927

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.