Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003586

Physical Location & Seq

Genomic Coordinates Forward (+)

46101682 .. 46115525

Gene Structure 13844 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1376800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGAAATCATCACAAATAAGTCCAAAAATCAAAAGAAATAAAAATCATGTTAAAAGAACTGTTATCGAACACGTGTCGTCTGTAACATCAAACTTGACCCAACTCTCTCGTATCCGTTCACGTGGCGGACATGAAAGTGCATCCCGCTTCCATGCCGCTCTCCCTCTCTTTCTCACAACCTCTCTCTCTTCACTCTCACTCTTCTTCCACTTTCCGACTTCCCTTCCACGTGATGTAGCTTCAGCTGGCTCAATCGAAACGTCACTAAAATTGTGCCCTCCTTTTGGTCCTAGCTTTCTCAGATTCATCATCTTTCTCGGAAAATTCGATCCCCTTTTGGCTCTCAATATACTCTCTCAGAACTTTTATCGTACACTTTGCAATCCAACGAAAAGATCAAGGATGCTTCGAACCCCACATTTGTACGATTCAAATGTCGCCAAGTTCCCTCTCGTTGCATCTCCCCATTCATCTCACCCTGTCGCTTCTTGCACACTAGCTTCTCCTGTTCTTGAAATGGGATTGAAGTGGAATAAACAAGCATCGGGTAGCAATCAGCAAGGAATTGTCAGAAAGACCCGGGATGGTGCAAAGCATAGCACAGCTAATGCTTACGGTCAGCGTGCTCAAGAACGTGTGCTTGAGGAAGAAGCCCCCCAACTGTCAGAATCCAGCTCATCATGGAAAACTTTCCCAGGTCAAGCTTTTCCGCTGGGTGTATCAGAAGTTGACAATGGGATAAATTTTGCTATCTTTTCACAGCATGCAACTGCTGTCACACTTTGCTTATCACTTCCAGAGAGGGAAAGGCTTGGAGGGCTGGATGGTGGAATGATGGAGCTGCCTTTGGATCCTAATGTTAACAAGACTGGTGACATTTGGCACATATGCATCAAGGATTTGCCCCGGAGCAATGTTCTTTATGGTTATCGTATGGATGGGCCACAAGGTTGGCATCAGGGACATCGATTTGATAGCAGCATTGTGCTTGTAGATCCTTATGCAAAGCTAGTTGAAGGCCGTCGGATGTTTGGAGACACCACGAAAAAGTTATCTAAATTTCTTGGAACTTATGATTTTGATAGCTTGCCTTTTGACTGGGGAGAAAACTACAAGCTCCCAAATATACCTGAGAAGGATCTTGTTATATATGAAATGAATGTTCGTGCATATACAGCTGATGAATCCAGTGGTTTGGATCCAGATATTCGTGGTAGCTACCTTGGTATGATCGAGAAGATTCCACACCTTCGAGAGCTTGGTATCAATGCAGTGGAATTGCTGCCTGTCTTCGAATTTGATGAGTTTGAGTTTCAGAGGCGACCAAATCCTAGAGATCACATGATAAATACCTGGGGCTACTCGACAATAAATTTCTTTGCTCCTATGAGCCGCTATGCAAGTGCTGGTGGAGGACCCGTTAAAGCTTCTCAGGAATTTAAACAAATGGTTAAAGCCTTGCATGGTGCTGGGATAGAGGTCATTTTGGATGTTGTCTACAATCATACTAATGAAGCTGACGATGCAAACCCCTATACAACTTCATTTCGTGGCATAGATAATAAGGTTTACTACATGCTGGACATGAACAACAATGGCCAATTACTGAACTACTCTGGCTGCGGGAACACATTAAACTGTAACCATCCTGTTGTCATGGAGCTCATTCTTGACAGCTTAAGACATTGGGTTGTTGAATATCATGTAGATGGGTTTAGATTTGACCTTGCCAGTGTTCTTTGTCGGGGAACAGATGGAACTCCTCTTGATGCACCACCAGTTATTAGGGCTATTGCAAAAGATGCTATCCTATCAAGATGTAAAATCATTTCCGAACCTTGGGATTGTGGAGGCCTTTATCTTGTTGGAAGATTTCCAAATTGGGACAGGTGGGCTGAATGGAATGGGAAATATCGTGATGATCTAAGGAGATTTATAAAGGGCGACTCTGGTATGAAGGGAAGTTTTGCAACTCGTGTTTCTGGGTCTGCTGACCTTTACAAAGTGAATAAGCGCAAACCCTATCACAGTGTTAATTTTATTATTGCGCATGATGGGTTCACTCTATATGATCTTGTTTCATACAATTTCAAGCACAACGAGGCTAATGGAGAAGGAGGAAATGATGGAAGTAATGATAATTTGAGCTGGAATTGTGGTCATGAAGGAGAAACTGATGATTCTACTATTAAAGCTATACGCTCCCGCCAAATGAAAAATTTTCATTTGGCATTAATGATATCCCAGGGAACCCCAATGATGCTGATGGGGGATGAATATGGGCATACTCGACATGGAAATAACAACAGTTATGGACATGATACTGCTCTTAACCATTTCCAGTGGGGACAGTTGGAGGCACGGAACAACAATCACTTCAGGTTTTTCAAGGAGGCAATAAAGTTTAGACGAGCACATCGTGTATTTGGTCGTGAAGCTTTTCTTGAGAAGCATGATGTGACATGGCATGAAGACAACTGGGACAACTATGAGAGCAAATTCCTTGCATTTACGTTTCATGAGAGTAGTGAAGGCGACATCTATTTGGCATTCAATGCTCATGACTGTTTTATTACAGTTCCAATACCGGCACCACCAGTAAAGAGGAGATGGTTTCGTGTGGTTGACACGAATCTGGAGTCTCCCAATGACATTGTGCCTGATGGCGTCCCGGGCATTGAAGGCACTTATAACATGGCCCCCTTCTCCTCAGTTCTTCTTCAAGCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009657 GO:0009660 GO:0009987 GO:0015980 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0071840 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

911

Amino Acids

102.68

Weight (kDa)

7.07

Isoelectric Point (pI)

40.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	237 - 335	2.6e-12	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	400 - 581	4.4e-11	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	823 - 889	3.9e-11	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09020
fragaria_vesca	FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440
malus_domestica	MD08G1188900.v1.1 MD15G1376800.v1.1
prunus_persica	Prupe.1G523500_v2.0.a1 Prupe.1G523500_v2.0.a1
pyrus_communis	pycom08g16260 pycom15g33660
rosa_chinensis	RchiOBHm_Chr7g0238421
rosa_laevigata	RLG00000000911
rosa_multiflora	Rmu_sc0031277.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223020
rosa_rugosa	Rorug07G0312600
rosa_samantha	Rh7AG467500 Rh7BG438200 Rh7CG485500 Rh7DG454000
rosa_wichuraiana	Rw7G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1937, 2691
Acc16I	TGCGCA	1 cut(s)	2049
AccB1I	GGYRCC	1 cut(s)	2590
AccBSI	CCGCTC	1 cut(s)	158
AccI	GTMKAC	1 cut(s)	1497
AciI	CCGC	7 cut(s)	126, 145, 156, 710, 1393, 1623, 2206
AclWI	GGATC	7 cut(s)	323, 845, 858, 989, 1146, 1193, 1206
AcoI	YGGCCR	1 cut(s)	1597
AcsI	RAATTY	8 cut(s)	323, 742, 1058, 1295, 1372, 1436, 2218, 2498
AcuI	CTGAAG	2 cut(s)	225, 2362
AcvI	CACGTG	3 cut(s)	73, 122, 230
AcyI	GRCGYC	1 cut(s)	2667
AdeI	CACNNNGTG	1 cut(s)	2420
AfaI	GTAC	2 cut(s)	373, 425
AfiI	CCNNNNNNNGG	2 cut(s)	284, 1839
AflII	CTTAAG	1 cut(s)	1678
AflIII	ACRYGT	3 cut(s)	70, 72, 634
AjnI	CCWGG	3 cut(s)	694, 1352, 2244
Alw21I	GWGCWC	3 cut(s)	628, 1665, 2416
Alw26I	GTCTC	2 cut(s)	1029, 2646
AlwI	GGATC	7 cut(s)	323, 845, 858, 989, 1146, 1193, 1206
Ama87I	CYCGRG	2 cut(s)	579, 2671
AoxI	GGCC	5 cut(s)	941, 1018, 1597, 1852, 2697
ApeKI	GCWGC	4 cut(s)	841, 978, 1282, 1620
ApoI	RAATTY	8 cut(s)	323, 742, 1058, 1295, 1372, 1436, 2218, 2498
ArsI	GACNNNNNNTTYG	2 cut(s)	2624, 2656
AseI	ATTAAT	1 cut(s)	2234
Asp700I	GAANNNNTTC	2 cut(s)	689, 2439
AspLEI	GCGC	2 cut(s)	2016, 2050
AspS9I	GGNCC	4 cut(s)	287, 941, 1415, 2698
AsuC2I	CCSGG	5 cut(s)	580, 581, 907, 2672, 2673
AsuHPI	GGTGA	2 cut(s)	467, 884
AsuII	TTCGAA	2 cut(s)	409, 1293
AvaI	CYCGRG	2 cut(s)	579, 2671
AvaII	GGWCC	2 cut(s)	287, 1415
BaeGI	GKGCMC	1 cut(s)	278
BalI	TGGCCA	1 cut(s)	1599
BamHI	GGATCC	2 cut(s)	850, 1198
BanI	GGYRCC	1 cut(s)	2590
BanII	GRGCYC	1 cut(s)	1665
BauI	CACGAG	1 cut(s)	1974
BbrPI	CACGTG	3 cut(s)	73, 122, 230
BbsI	GAAGAC	2 cut(s)	1282, 2478
Bbv12I	GWGCWC	3 cut(s)	628, 1665, 2416
BbvI	GCAGC	4 cut(s)	828, 990, 1269, 1607
BceAI	ACGGC	1 cut(s)	1005
BciT130I	CCWGG	3 cut(s)	696, 1354, 2246
BciVI	GTATCC	1 cut(s)	122
BcnI	CCSGG	5 cut(s)	580, 581, 907, 2672, 2673
BcoDI	GTCTC	2 cut(s)	1029, 2646
BfaI	CTAG	4 cut(s)	291, 497, 1010, 1332
BfrI	CTTAAG	1 cut(s)	1678
BfuI	GTATCC	1 cut(s)	122
BisI	GCNGC	6 cut(s)	156, 842, 979, 1283, 1393, 1621
BlsI	GCNGC	6 cut(s)	157, 843, 980, 1284, 1394, 1622
Bme1390I	CCNGG	8 cut(s)	580, 581, 696, 907, 1354, 2246, 2672, 2673
Bme18I	GGWCC	2 cut(s)	287, 1415
BmeT110I	CYCGRG	2 cut(s)	579, 2671
BmgT120I	GGNCC	4 cut(s)	287, 941, 1415, 2698
BmiI	GGNNCC	6 cut(s)	852, 1200, 1417, 2251, 2592, 2700
BmrFI	CCNGG	8 cut(s)	580, 581, 696, 907, 1354, 2246, 2672, 2673
BmrI	ACTGGG	2 cut(s)	1108, 2488
BmuI	ACTGGG	2 cut(s)	1108, 2488
BpiI	GAAGAC	2 cut(s)	1282, 2478
BpmI	CTGGAG	1 cut(s)	2657
Bpu14I	TTCGAA	2 cut(s)	409, 1293
BpuEI	CTTGAG	3 cut(s)	612, 662, 2465
BpuMI	CCSGG	5 cut(s)	580, 581, 907, 2672, 2673
Bsa29I	ATCGAT	1 cut(s)	967
BsaAI	YACGTR	3 cut(s)	73, 122, 230
BsaHI	GRCGYC	1 cut(s)	2667
BsaJI	CCNNGG	9 cut(s)	579, 694, 905, 1222, 1353, 1838, 2244, 2245, 2671
BsaXI	ACNNNNNCTCC	2 cut(s)	1404, 1434
Bsc4I	CCNNNNNNNGG	2 cut(s)	284, 1839
Bse118I	RCCGGY	1 cut(s)	2587
Bse1I	ACTGG	8 cut(s)	874, 1103, 1188, 1731, 1778, 2341, 2483, 2597
Bse3DI	GCAATG	1 cut(s)	919
BseBI	CCWGG	3 cut(s)	696, 1354, 2246
BseCI	ATCGAT	1 cut(s)	967
BseDI	CCNNGG	9 cut(s)	579, 694, 905, 1222, 1353, 1838, 2244, 2245, 2671
BseGI	GGATG	9 cut(s)	140, 408, 589, 829, 943, 1032, 1495, 1645, 2278
BseLI	CCNNNNNNNGG	2 cut(s)	284, 1839
BseMI	GCAATG	1 cut(s)	919
BseMII	CTCAG	5 cut(s)	313, 371, 1122, 1445, 2724
BseNI	ACTGG	8 cut(s)	874, 1103, 1188, 1731, 1778, 2341, 2483, 2597
BseRI	GAGGAG	3 cut(s)	1752, 2620, 2698
BseSI	GKGCMC	1 cut(s)	278
BseXI	GCAGC	4 cut(s)	828, 990, 1269, 1607
BseYI	CCCAGC	2 cut(s)	712, 1469
BshFI	GGCC	5 cut(s)	943, 1020, 1599, 1854, 2699
BshNI	GGYRCC	1 cut(s)	2590
BshVI	ATCGAT	1 cut(s)	967
BsiHKAI	GWGCWC	3 cut(s)	628, 1665, 2416
BsiHKCI	CYCGRG	2 cut(s)	579, 2671
BsiSI	CCGG	4 cut(s)	580, 907, 2588, 2672
BslFI	GGGAC	5 cut(s)	975, 1898, 2361, 2495, 2654
BslI	CCNNNNNNNGG	2 cut(s)	284, 1839
BsmAI	GTCTC	2 cut(s)	1029, 2646
BsmFI	GGGAC	5 cut(s)	975, 1898, 2361, 2495, 2654
BsmI	GAATGC	1 cut(s)	2549
BsnI	GGCC	5 cut(s)	943, 1020, 1599, 1854, 2699
BsoBI	CYCGRG	2 cut(s)	579, 2671
Bsp119I	TTCGAA	2 cut(s)	409, 1293
Bsp1286I	GDGCHC	4 cut(s)	278, 628, 1665, 2416
BspACI	CCGC	7 cut(s)	126, 145, 156, 710, 1393, 1623, 2206
BspANI	GGCC	5 cut(s)	943, 1020, 1599, 1854, 2699
BspCNI	CTCAG	5 cut(s)	312, 370, 1123, 1444, 2723
BspDI	ATCGAT	1 cut(s)	967
BspHI	TCATGA	3 cut(s)	2161, 2518, 2560
BspLI	GGNNCC	6 cut(s)	852, 1200, 1417, 2251, 2592, 2700
BspPI	GGATC	7 cut(s)	323, 845, 858, 989, 1146, 1193, 1206
BspT104I	TTCGAA	2 cut(s)	409, 1293
BspT107I	GGYRCC	1 cut(s)	2590
BspTI	CTTAAG	1 cut(s)	1678
BsrBI	CCGCTC	1 cut(s)	158
BsrDI	GCAATG	1 cut(s)	919
BsrFI	RCCGGY	1 cut(s)	2587
BsrI	ACTGG	8 cut(s)	874, 1103, 1188, 1731, 1778, 2341, 2483, 2597
BssAI	RCCGGY	1 cut(s)	2587
BssECI	CCNNGG	9 cut(s)	579, 694, 905, 1222, 1353, 1838, 2244, 2245, 2671
BssNI	GRCGYC	1 cut(s)	2667
BssSI	CACGAG	1 cut(s)	1974
BssT1I	CCWWGG	2 cut(s)	1222, 1838
Bst2BI	CACGAG	1 cut(s)	1974
Bst2UI	CCWGG	3 cut(s)	696, 1354, 2246
Bst4CI	ACNGT	9 cut(s)	61, 617, 663, 1640, 2030, 2309, 2352, 2567, 2578
Bst6I	CTCTTC	2 cut(s)	193, 206
BstACI	GRCGYC	1 cut(s)	2667
BstAFI	CTTAAG	1 cut(s)	1678
BstAPI	GCANNNNNTGC	2 cut(s)	1802, 2555
BstBAI	YACGTR	3 cut(s)	73, 122, 230
BstBI	TTCGAA	2 cut(s)	409, 1293
BstC8I	GCNNGC	4 cut(s)	247, 624, 765, 1088
BstDEI	CTNAG	6 cut(s)	299, 357, 1131, 1431, 1925, 2710
BstF5I	GGATG	9 cut(s)	140, 408, 589, 829, 943, 1032, 1495, 1645, 2278
BstHHI	GCGC	2 cut(s)	2016, 2050
BstMAI	GTCTC	2 cut(s)	1029, 2646
BstMWI	GCNNNNNNNGC	7 cut(s)	817, 1175, 1523, 1802, 2555, 2673, 2682
BstNI	CCWGG	3 cut(s)	696, 1354, 2246
BstNSI	RCATGY	2 cut(s)	767, 1579
BstSCI	CCNGG	8 cut(s)	578, 579, 694, 905, 1352, 2244, 2670, 2671
BstSLI	GKGCMC	1 cut(s)	278
BstV1I	GCAGC	4 cut(s)	828, 990, 1269, 1607
BstV2I	GAAGAC	2 cut(s)	1282, 2478
BstX2I	RGATCY	4 cut(s)	850, 994, 1138, 1198
BstXI	CCANNNNNNTGG	2 cut(s)	951, 1195
BstYI	RGATCY	4 cut(s)	850, 994, 1138, 1198
Bsu15I	ATCGAT	1 cut(s)	967
BsuI	GTATCC	1 cut(s)	122
BsuRI	GGCC	5 cut(s)	943, 1020, 1599, 1854, 2699
BsuTUI	ATCGAT	1 cut(s)	967
BtsCI	GGATG	9 cut(s)	140, 408, 589, 829, 943, 1032, 1495, 1645, 2278
BtsI	GCAGTG	1 cut(s)	1278
BtsIMutI	CAGTG	5 cut(s)	1195, 1278, 1738, 2035, 2348
Cac8I	GCNNGC	4 cut(s)	247, 624, 765, 1088
CciI	TCATGA	3 cut(s)	2161, 2518, 2560
CfoI	GCGC	2 cut(s)	2016, 2050
Cfr10I	RCCGGY	1 cut(s)	2587
Cfr13I	GGNCC	4 cut(s)	287, 941, 1415, 2698
Cfr9I	CCCGGG	2 cut(s)	579, 2671
ClaI	ATCGAT	1 cut(s)	967
CseI	GACGC	1 cut(s)	2656
Csp6I	GTAC	2 cut(s)	372, 424
CviQI	GTAC	2 cut(s)	372, 424
DdeI	CTNAG	6 cut(s)	299, 357, 1131, 1431, 1925, 2710
DraI	TTTAAA	1 cut(s)	1441
DraIII	CACNNNGTG	1 cut(s)	2420
EaeI	YGGCCR	1 cut(s)	1597
Eam1104I	CTCTTC	2 cut(s)	193, 206
EarI	CTCTTC	2 cut(s)	193, 206
EciI	GGCGGA	1 cut(s)	141
Ecl136II	GAGCTC	1 cut(s)	1663
Eco130I	CCWWGG	2 cut(s)	1222, 1838
Eco147I	AGGCCT	1 cut(s)	1854
Eco24I	GRGCYC	1 cut(s)	1665
Eco32I	GATATC	1 cut(s)	2241
Eco47I	GGWCC	2 cut(s)	287, 1415
Eco53kI	GAGCTC	1 cut(s)	1663
Eco57I	CTGAAG	2 cut(s)	225, 2362
Eco72I	CACGTG	3 cut(s)	73, 122, 230
Eco88I	CYCGRG	2 cut(s)	579, 2671
EcoICRI	GAGCTC	1 cut(s)	1663
EcoO109I	RGGNCCY	1 cut(s)	1415
EcoRII	CCWGG	3 cut(s)	694, 1352, 2244
EcoRV	GATATC	1 cut(s)	2241
EcoT14I	CCWWGG	2 cut(s)	1222, 1838
EcoT22I	ATGCAT	1 cut(s)	893
EcoT38I	GRGCYC	1 cut(s)	1665
ErhI	CCWWGG	2 cut(s)	1222, 1838
FalI	AAGNNNNNCTT	2 cut(s)	2427, 2459
FaqI	GGGAC	5 cut(s)	975, 1898, 2361, 2495, 2654
FauI	CCCGC	3 cut(s)	152, 1616, 2213
FauNDI	CATATG	1 cut(s)	887
FblI	GTMKAC	1 cut(s)	1497
Fnu4HI	GCNGC	6 cut(s)	156, 842, 979, 1283, 1393, 1621
FokI	GGATG	9 cut(s)	127, 415, 596, 836, 950, 1039, 1502, 1632, 2285
FriOI	GRGCYC	1 cut(s)	1665
Fsp4HI	GCNGC	6 cut(s)	156, 842, 979, 1283, 1393, 1621
FspBI	CTAG	4 cut(s)	291, 497, 1010, 1332
FspI	TGCGCA	1 cut(s)	2049
GlaI	GCGC	2 cut(s)	2015, 2049
GluI	GCNGC	6 cut(s)	156, 842, 979, 1283, 1393, 1621
GsaI	CCCAGC	2 cut(s)	716, 1473
GsuI	CTGGAG	1 cut(s)	2657
HaeIII	GGCC	5 cut(s)	943, 1020, 1599, 1854, 2699
HapII	CCGG	4 cut(s)	580, 907, 2588, 2672
HgaI	GACGC	1 cut(s)	2656
HhaI	GCGC	2 cut(s)	2016, 2050
Hin1I	GRCGYC	1 cut(s)	2667
Hin6I	GCGC	2 cut(s)	2014, 2048
HinP1I	GCGC	2 cut(s)	2014, 2048
HincII	GTYRAC	3 cut(s)	730, 862, 2626
HindII	GTYRAC	3 cut(s)	730, 862, 2626
HindIII	AAGCTT	3 cut(s)	702, 1425, 2436
HinfI	GANTC	9 cut(s)	303, 428, 668, 1184, 1240, 1946, 2180, 2632, 2639
HpaI	GTTAAC	1 cut(s)	862
HpaII	CCGG	4 cut(s)	580, 907, 2588, 2672
HphI	GGTGA	2 cut(s)	467, 884
Hpy166II	GTNNAC	8 cut(s)	119, 374, 730, 862, 1498, 1570, 2061, 2626
Hpy8I	GTNNAC	8 cut(s)	119, 374, 730, 862, 1498, 1570, 2061, 2626
Hpy99I	CGWCG	1 cut(s)	1026
HpyCH4III	ACNGT	9 cut(s)	61, 617, 663, 1640, 2030, 2309, 2352, 2567, 2578
HpyCH4IV	ACGT	6 cut(s)	72, 121, 229, 260, 634, 2513
HpyF10VI	GCNNNNNNNGC	7 cut(s)	817, 1175, 1523, 1802, 2555, 2673, 2682
HpyF3I	CTNAG	6 cut(s)	299, 357, 1131, 1431, 1925, 2710
HpySE526I	ACGT	6 cut(s)	72, 121, 229, 260, 634, 2513
Hsp92I	GRCGYC	1 cut(s)	2667
HspAI	GCGC	2 cut(s)	2014, 2048
KspAI	GTTAAC	1 cut(s)	862
LmnI	GCTCC	6 cut(s)	838, 909, 1122, 1387, 1660, 2207
Lsp1109I	GCAGC	4 cut(s)	828, 990, 1269, 1607
MaeI	CTAG	4 cut(s)	291, 497, 1010, 1332
MaeII	ACGT	6 cut(s)	72, 121, 229, 260, 634, 2513
MaeIII	GTNAC	6 cut(s)	82, 261, 775, 872, 1640, 2458
MbiI	CCGCTC	1 cut(s)	158
MflI	RGATCY	4 cut(s)	850, 994, 1138, 1198
MhlI	GDGCHC	4 cut(s)	278, 628, 1665, 2416
MlsI	TGGCCA	1 cut(s)	1599
MluNI	TGGCCA	1 cut(s)	1599
MlyI	GAGTC	2 cut(s)	1940, 2648
MmeI	TCCRAC	5 cut(s)	239, 410, 1004, 1846, 2334
Mox20I	TGGCCA	1 cut(s)	1599
Mph1103I	ATGCAT	1 cut(s)	893
MroXI	GAANNNNTTC	2 cut(s)	689, 2439
MscI	TGGCCA	1 cut(s)	1599
MslI	CAYNNNNRTG	3 cut(s)	135, 1509, 1707
Msp20I	TGGCCA	1 cut(s)	1599
MspA1I	CMGCKG	3 cut(s)	245, 712, 1178
MspCI	CTTAAG	1 cut(s)	1678
MspI	CCGG	4 cut(s)	580, 907, 2588, 2672
MspR9I	CCNGG	8 cut(s)	580, 581, 696, 907, 1354, 2246, 2672, 2673
Mva1269I	GAATGC	1 cut(s)	2549
MvaI	CCWGG	3 cut(s)	696, 1354, 2246
MwoI	GCNNNNNNNGC	7 cut(s)	817, 1175, 1523, 1802, 2555, 2673, 2682
NciI	CCSGG	5 cut(s)	580, 581, 907, 2672, 2673
NdeI	CATATG	1 cut(s)	887
NlaIV	GGNNCC	6 cut(s)	852, 1200, 1417, 2251, 2592, 2700
NmuCI	GTSAC	4 cut(s)	261, 775, 872, 2458
NsbI	TGCGCA	1 cut(s)	2049
NsiI	ATGCAT	1 cut(s)	893
NspI	RCATGY	2 cut(s)	767, 1579
NspV	TTCGAA	2 cut(s)	409, 1293
PaeI	GCATGC	1 cut(s)	767
PagI	TCATGA	3 cut(s)	2161, 2518, 2560
PasI	CCCWGGG	1 cut(s)	2245
PceI	AGGCCT	1 cut(s)	1854
PcsI	WCGNNNNNNNCGW	2 cut(s)	324, 2416
PctI	GAATGC	1 cut(s)	2549
PdmI	GAANNNNTTC	2 cut(s)	689, 2439
PfeI	GAWTC	7 cut(s)	303, 428, 668, 1184, 1240, 2180, 2632
PkrI	GCNGC	6 cut(s)	157, 843, 980, 1284, 1394, 1622
PleI	GAGTC	2 cut(s)	1940, 2647
PmaCI	CACGTG	3 cut(s)	73, 122, 230
PmlI	CACGTG	3 cut(s)	73, 122, 230
PpsI	GAGTC	2 cut(s)	1940, 2647
Ppu21I	YACGTR	3 cut(s)	73, 122, 230
PpuMI	RGGWCCY	1 cut(s)	1415
PshBI	ATTAAT	1 cut(s)	2234
PsiI	TTATAA	2 cut(s)	1937, 2691
Psp124BI	GAGCTC	1 cut(s)	1665
Psp5II	RGGWCCY	1 cut(s)	1415
Psp6I	CCWGG	3 cut(s)	694, 1352, 2244
PspCI	CACGTG	3 cut(s)	73, 122, 230
PspFI	CCCAGC	2 cut(s)	712, 1469
PspGI	CCWGG	3 cut(s)	694, 1352, 2244
PspN4I	GGNNCC	6 cut(s)	852, 1200, 1417, 2251, 2592, 2700
PspPI	GGNCC	4 cut(s)	287, 941, 1415, 2698
PspPPI	RGGWCCY	1 cut(s)	1415
PsuI	RGATCY	4 cut(s)	850, 994, 1138, 1198
PvuII	CAGCTG	2 cut(s)	245, 1178
RsaI	GTAC	2 cut(s)	373, 425
RsaNI	GTAC	2 cut(s)	372, 424
RseI	CAYNNNNRTG	3 cut(s)	135, 1509, 1707
SacI	GAGCTC	1 cut(s)	1665
SatI	GCNGC	6 cut(s)	156, 842, 979, 1283, 1393, 1621
Sau96I	GGNCC	4 cut(s)	287, 941, 1415, 2698
SchI	GAGTC	2 cut(s)	1940, 2648
ScrFI	CCNGG	8 cut(s)	580, 581, 696, 907, 1354, 2246, 2672, 2673
SduI	GDGCHC	4 cut(s)	278, 628, 1665, 2416
SfuI	TTCGAA	2 cut(s)	409, 1293
SinI	GGWCC	2 cut(s)	287, 1415
SmaI	CCCGGG	2 cut(s)	581, 2673
SmiMI	CAYNNNNRTG	3 cut(s)	135, 1509, 1707
SmlI	CTYRAG	4 cut(s)	627, 641, 1678, 2444
SmoI	CTYRAG	4 cut(s)	627, 641, 1678, 2444
SphI	GCATGC	1 cut(s)	767
SseBI	AGGCCT	1 cut(s)	1854
SsiI	CCGC	7 cut(s)	126, 145, 156, 710, 1393, 1623, 2206
SspMI	CTAG	4 cut(s)	291, 497, 1010, 1332
SstI	GAGCTC	1 cut(s)	1665
StuI	AGGCCT	1 cut(s)	1854
StyD4I	CCNGG	8 cut(s)	578, 579, 694, 905, 1352, 2244, 2670, 2671
StyI	CCWWGG	2 cut(s)	1222, 1838
TaaI	ACNGT	9 cut(s)	61, 617, 663, 1640, 2030, 2309, 2352, 2567, 2578
TaiI	ACGT	6 cut(s)	75, 124, 232, 263, 637, 2516
TauI	GCSGC	2 cut(s)	158, 1395
TfiI	GAWTC	7 cut(s)	303, 428, 668, 1184, 1240, 2180, 2632
TscAI	CASTG	5 cut(s)	1195, 1278, 1738, 2035, 2348
TseFI	GTSAC	4 cut(s)	261, 775, 872, 2458
TseI	GCWGC	4 cut(s)	841, 978, 1282, 1620
Tsp45I	GTSAC	4 cut(s)	261, 775, 872, 2458
TspGWI	ACGGA	2 cut(s)	104, 2377
TspMI	CCCGGG	2 cut(s)	579, 2671
TspRI	CASTG	5 cut(s)	1195, 1278, 1738, 2035, 2348
Vha464I	CTTAAG	1 cut(s)	1678
VpaK11BI	GGWCC	2 cut(s)	287, 1415
VspI	ATTAAT	1 cut(s)	2234
XapI	RAATTY	8 cut(s)	323, 742, 1058, 1295, 1372, 1436, 2218, 2498
XceI	RCATGY	2 cut(s)	767, 1579
XmaI	CCCGGG	2 cut(s)	579, 2671
XmiI	GTMKAC	1 cut(s)	1497
XmnI	GAANNNNTTC	2 cut(s)	689, 2439
XspI	CTAG	4 cut(s)	291, 497, 1010, 1332
Zsp2I	ATGCAT	1 cut(s)	893

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.