Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0003586

Physical Location & Seq

Genomic Coordinates Forward (+)

16146203 .. 16157703

Gene Structure 11501 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g16260.13

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGATGCTTCGAACCCCACATTTGTACGATTCAAATACCTCCATGTTCCCTCTCTTTGCATCTCCGCCTTCATCTCACGCTAGTGCTTCATGCACAGTGGCTTCTTCTGTCATTGAAATGGGATTGAAGTGGAGTAAACATGCATCGGGTAGCAATCAGCAAGGAGCTGTCAGAAAGACCAGGAATGGGGCCATGCACAGTACAGCCAGTGCTTACGGTCGGCGTGCTCAAGAACGTGTACTTGAGGAAGAAGCAACCCAACTGTCAGAATCCAGCCCATCATGGAAACTTTTCCCAGGTCAAGCTTTTCCGTTGGGTGTATCAGAAGTTGACAGTGGGATAAATTTTGCTATCTTTTCACAGCATGCAACAGCTGTCACACTATGCTTATCATTTCCAGAGAGGGTAAGCCTTGGAGGCCTGGATGGTGGAATGATGGAGCTGCCTTTGGATCGTAATGTTAACAAGACCGGTGACATTTGGCACATATGTATCCAGGATTTTCCGAGGAGCAATGTTCTTTATGGTTATCGTATGGATGGGCCACGAGGTTGGCATCAGGGACATCGATTTGACAGCAGCATTGTGCTTGTGGATCCTTACGCAAAGCTAGTTGAAGGCCGTCGAATTTTTGGAGATACCCCAAAAAAGTTATCTAAATTTCTTGGAACTTATGATTTTGATAGCTTGCCTTTTTACTGGGGAGACAACTACAAGCTCCCAAATGTACCTGAGAAGGATCTTGTTATCTACGAAATGAATGTTCGTGCATATACAGCTGATGAATCCAGCGGTTTGGATCCTGATATACGTGGTAGCTACCTTGGTGTGATCCAGAAGATTTCACACCTTCGAGAGCTTGGTATCAATGCAGTGCAACTGCTGCCGGTTTTCGAATTTGATGAGTTTGAGTTCCAGAGGAGACCAAATCCTAGAGATCACATGATAAACACATGGGGCTACTCGACAATAAATTTCTTTGCTCCTATGAGCCGCTATGCAAGTGCTGGTGGAGGGCCCCTTAAAGCTTCTCAGGAATTTAAACAAATGGTTAAAGCCTTGCATGGTGCTGGGATAGAGGTCATTTTGGATGTTGTCTATAATCATACTAATGAAGCTGATGATGCCAACCCCTATACAACTTCATTTCGCGGCGTAGATAATAAGGTTTACTACATGCTGGACGTAAACAACAATGGCCAATTACTTAACTACACTGGCTGCGGGAACACATTAAACTGTAACCATCCTGTTGTCATGGAGCTCATTCTTGACAGTTTAAGACATTGGGTTACTGAATATCATGTGGATGGATTTAGATTTGACCTTGCCAGTGTTCTTTGTCGGGGAGCAGATGGAACTCCTCTTGACGCACCACCACTTATTAGGGCTATTGCAAAAGATGCCATCTTATCAAGATGTAAAATCATTTCCGAACCTTGGGATTGTGGAGGCCTTTATCTTGTTGGAAGATTTCCAAATTGGGACAGGTGGGCTGAATGGAATGGGAAATATCGTGATGACTTAAGGAGATTTATAAAGGGTGACTCTGGTATGAAAGGAAGTTTTGCCACCCGCATTTCTGGATCTGCTGACCTTTACAAAGTGAATAAGCGCAAACCGTATCACAGCGTTAATTTTATTATTGCGCATGATGGGTTCACACTATATGATCTTGTTTCATACAATTTCAAGCACAATGATGCTAATGGAGAAGGAGGAAATGATGGAAATAACGATAATTTGAGCTGGAATTGTGGTCATGAAGGAGAAACTGATGATTCTACTGTTAAAGCTATTCGCTCCCGCCAAATGAAAAACTTTCATTTGGCATTAATGATATCCCAGGGAACCCCAATGATGCTGATGGGGGATGAATATGGGCATACTCGACACGGAAATAACAACAGTTATGGGCATGATACTGCTCTTAACCATTTCCAGTGGGGACTGTTGAAGGCACGGAACAACAATCGCTTCAGGTTTTTCAAGGAGGTGATAAAGTTTCGACAAGCACATCGTGTATTTGGTCGTGATACATTTCTTGAGAAACATGATGTGACATGGCATGAAGACAACTGGTACAACTATGAGAGCAAATTCCTTGCATTTACGTTTCATGACAATAGTGAAGGCGACGTCTATTTGGCATTCAATGCACATGACTATTTTATTAAAGTTCCAATGCCCGCACCACCGGTCAAGAGGAAATGGTTTCGTGTGGTTGACACTAATTTGGAGTCTCCCAATGACATCGTGCCTGAAGGCGTCCCGGGCATTGCAGGCACTTACAACATGGCCCCCTTCTCCTCAATTCTTCTCCAAGCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009657 GO:0009660 GO:0009987 GO:0015980 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0071840 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

778

Amino Acids

87.83

Weight (kDa)

6.5

Isoelectric Point (pI)

34.32

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09020
fragaria_vesca	FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440
malus_domestica	MD08G1188900.v1.1 MD15G1376800.v1.1
prunus_persica	Prupe.1G523500_v2.0.a1 Prupe.1G523500_v2.0.a1
pyrus_communis	pycom08g16260 pycom15g33660
rosa_chinensis	RchiOBHm_Chr7g0238421
rosa_laevigata	RLG00000000911
rosa_multiflora	Rmu_sc0031277.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223020
rosa_rugosa	Rorug07G0312600
rosa_samantha	Rh7AG467500 Rh7BG438200 Rh7CG485500 Rh7DG454000
rosa_wichuraiana	Rw7G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1538
AatII	GACGTC	1 cut(s)	2142
Acc16I	TGCGCA	1 cut(s)	1650
AccII	CGCG	1 cut(s)	1152
AciI	CCGC	8 cut(s)	65, 792, 994, 1152, 1224, 1576, 1807, 2190
AclWI	GGATC	8 cut(s)	459, 590, 603, 747, 794, 807, 826, 1594
AcoI	YGGCCR	1 cut(s)	1198
AcsI	RAATTY	7 cut(s)	343, 627, 659, 896, 973, 1037, 2099
AcuI	CTGAAG	2 cut(s)	1963, 2283
AcyI	GRCGYC	2 cut(s)	2139, 2268
AdeI	CACNNNGTG	1 cut(s)	2021
AfaI	GTAC	5 cut(s)	26, 202, 240, 729, 2084
AfiI	CCNNNNNNNGG	2 cut(s)	186, 1440
AflII	CTTAAG	1 cut(s)	1525
AflIII	ACRYGT	1 cut(s)	235
AgeI	ACCGGT	2 cut(s)	470, 2197
AgsI	TTSAA	8 cut(s)	33, 116, 127, 617, 1693, 1957, 1990, 2155
AjnI	CCWGG	5 cut(s)	179, 295, 420, 495, 1845
AleI	CACNNNNGTG	1 cut(s)	81
Alw21I	GWGCWC	2 cut(s)	229, 1266
Alw26I	GTCTC	3 cut(s)	699, 916, 2247
AlwI	GGATC	8 cut(s)	459, 590, 603, 747, 794, 807, 826, 1594
Ama87I	CYCGRG	1 cut(s)	2272
AoxI	GGCC	8 cut(s)	189, 418, 542, 619, 1016, 1198, 1453, 2298
ApaI	GGGCCC	1 cut(s)	1020
ApeKI	GCWGC	4 cut(s)	442, 579, 883, 1221
ApoI	RAATTY	7 cut(s)	343, 627, 659, 896, 973, 1037, 2099
AseI	ATTAAT	1 cut(s)	1835
AsiGI	ACCGGT	2 cut(s)	470, 2197
Asp700I	GAANNNNTTC	1 cut(s)	290
AspLEI	GCGC	2 cut(s)	1617, 1651
AspS9I	GGNCC	5 cut(s)	189, 542, 1016, 1017, 2299
AsuC2I	CCSGG	2 cut(s)	2273, 2274
AsuHPI	GGTGA	3 cut(s)	485, 1556, 2008
AsuII	TTCGAA	2 cut(s)	10, 894
AvaI	CYCGRG	1 cut(s)	2272
BaeGI	GKGCMC	1 cut(s)	1020
BaeI	ACNNNNGTAYC	2 cut(s)	475, 508
BalI	TGGCCA	1 cut(s)	1200
BamHI	GGATCC	2 cut(s)	595, 799
BanII	GRGCYC	2 cut(s)	1020, 1266
BauI	CACGAG	1 cut(s)	546
BbsI	GAAGAC	1 cut(s)	2079
Bbv12I	GWGCWC	2 cut(s)	229, 1266
BbvI	GCAGC	4 cut(s)	429, 591, 870, 1208
BceAI	ACGGC	1 cut(s)	606
BciT130I	CCWGG	5 cut(s)	181, 297, 422, 497, 1847
BciVI	GTATCC	1 cut(s)	503
BcnI	CCSGG	2 cut(s)	2273, 2274
BcoDI	GTCTC	3 cut(s)	699, 916, 2247
BfaI	CTAG	3 cut(s)	81, 611, 933
BfrI	CTTAAG	1 cut(s)	1525
BfuI	GTATCC	1 cut(s)	503
BglI	GCCNNNNNGGC	1 cut(s)	417
BisI	GCNGC	6 cut(s)	443, 580, 884, 994, 1153, 1222
BlsI	GCNGC	6 cut(s)	444, 581, 885, 995, 1154, 1223
Bme1390I	CCNGG	7 cut(s)	181, 297, 422, 497, 1847, 2273, 2274
BmeT110I	CYCGRG	1 cut(s)	2272
BmgT120I	GGNCC	5 cut(s)	189, 542, 1016, 1017, 2299
BmiI	GGNNCC	7 cut(s)	190, 597, 801, 1018, 1019, 1852, 2301
BmrFI	CCNGG	7 cut(s)	181, 297, 422, 497, 1847, 2273, 2274
BmrI	ACTGGG	1 cut(s)	709
BmsI	GCATC	7 cut(s)	68, 152, 565, 1114, 1393, 1693, 1851
BmuI	ACTGGG	1 cut(s)	709
BpiI	GAAGAC	1 cut(s)	2079
Bpu14I	TTCGAA	2 cut(s)	10, 894
BpuEI	CTTGAG	3 cut(s)	213, 263, 2066
BpuMI	CCSGG	2 cut(s)	2273, 2274
Bsa29I	ATCGAT	1 cut(s)	568
BsaAI	YACGTR	1 cut(s)	812
BsaHI	GRCGYC	2 cut(s)	2139, 2268
BsaI	GGTCTC	1 cut(s)	916
BsaJI	CCNNGG	8 cut(s)	295, 412, 506, 823, 1439, 1845, 1846, 2272
BsaWI	WCCGGW	2 cut(s)	470, 2197
Bsc4I	CCNNNNNNNGG	2 cut(s)	186, 1440
Bse118I	RCCGGY	3 cut(s)	470, 886, 2197
Bse1I	ACTGG	6 cut(s)	207, 704, 1222, 1332, 1942, 2084
Bse3DI	GCAATG	2 cut(s)	520, 2277
BseBI	CCWGG	5 cut(s)	181, 297, 422, 497, 1847
BseCI	ATCGAT	1 cut(s)	568
BseDI	CCNNGG	8 cut(s)	295, 412, 506, 823, 1439, 1845, 1846, 2272
BseGI	GGATG	6 cut(s)	430, 544, 1096, 1246, 1315, 1879
BseLI	CCNNNNNNNGG	2 cut(s)	186, 1440
BseMI	GCAATG	2 cut(s)	520, 2277
BseMII	CTCAG	2 cut(s)	723, 1046
BseNI	ACTGG	6 cut(s)	207, 704, 1222, 1332, 1942, 2084
BseRI	GAGGAG	4 cut(s)	523, 934, 1353, 2299
BseSI	GKGCMC	1 cut(s)	1020
BseXI	GCAGC	4 cut(s)	429, 591, 870, 1208
BseYI	CCCAGC	1 cut(s)	1070
Bsh1236I	CGCG	1 cut(s)	1152
Bsh1285I	CGRYCG	1 cut(s)	220
BshFI	GGCC	8 cut(s)	191, 420, 544, 621, 1018, 1200, 1455, 2300
BshTI	ACCGGT	2 cut(s)	470, 2197
BshVI	ATCGAT	1 cut(s)	568
BsiEI	CGRYCG	1 cut(s)	220
BsiHKAI	GWGCWC	2 cut(s)	229, 1266
BsiHKCI	CYCGRG	1 cut(s)	2272
BsiSI	CCGG	4 cut(s)	471, 887, 2198, 2273
BslFI	GGGAC	4 cut(s)	576, 1499, 1962, 2255
BslI	CCNNNNNNNGG	2 cut(s)	186, 1440
BsmAI	GTCTC	3 cut(s)	699, 916, 2247
BsmFI	GGGAC	4 cut(s)	576, 1499, 1962, 2255
BsmI	GAATGC	1 cut(s)	2150
BsnI	GGCC	8 cut(s)	191, 420, 544, 621, 1018, 1200, 1455, 2300
Bso31I	GGTCTC	1 cut(s)	916
BsoBI	CYCGRG	1 cut(s)	2272
Bsp119I	TTCGAA	2 cut(s)	10, 894
Bsp120I	GGGCCC	1 cut(s)	1016
Bsp1286I	GDGCHC	3 cut(s)	229, 1020, 1266
Bsp143I	GATC	8 cut(s)	451, 595, 739, 799, 831, 937, 1586, 1672
BspACI	CCGC	8 cut(s)	65, 792, 994, 1152, 1224, 1576, 1807, 2190
BspANI	GGCC	8 cut(s)	191, 420, 544, 621, 1018, 1200, 1455, 2300
BspCNI	CTCAG	2 cut(s)	724, 1045
BspDI	ATCGAT	1 cut(s)	568
BspFNI	CGCG	1 cut(s)	1152
BspHI	TCATGA	2 cut(s)	1762, 2119
BspLI	GGNNCC	7 cut(s)	190, 597, 801, 1018, 1019, 1852, 2301
BspPI	GGATC	8 cut(s)	459, 590, 603, 747, 794, 807, 826, 1594
BspT104I	TTCGAA	2 cut(s)	10, 894
BspTI	CTTAAG	1 cut(s)	1525
BspTNI	GGTCTC	1 cut(s)	916
BsrDI	GCAATG	2 cut(s)	520, 2277
BsrFI	RCCGGY	3 cut(s)	470, 886, 2197
BsrI	ACTGG	6 cut(s)	207, 704, 1222, 1332, 1942, 2084
BssAI	RCCGGY	3 cut(s)	470, 886, 2197
BssECI	CCNNGG	8 cut(s)	295, 412, 506, 823, 1439, 1845, 1846, 2272
BssMI	GATC	8 cut(s)	451, 595, 739, 799, 831, 937, 1586, 1672
BssNI	GRCGYC	2 cut(s)	2139, 2268
BssSI	CACGAG	1 cut(s)	546
BssT1I	CCWWGG	3 cut(s)	412, 823, 1439
Bst2BI	CACGAG	1 cut(s)	546
Bst2UI	CCWGG	5 cut(s)	181, 297, 422, 497, 1847
BstACI	GRCGYC	2 cut(s)	2139, 2268
BstAFI	CTTAAG	1 cut(s)	1525
BstAPI	GCANNNNNTGC	3 cut(s)	883, 1403, 2156
BstBAI	YACGTR	1 cut(s)	812
BstBI	TTCGAA	2 cut(s)	10, 894
BstC8I	GCNNGC	5 cut(s)	225, 366, 689, 2190, 2284
BstDEI	CTNAG	2 cut(s)	732, 1032
BstF5I	GGATG	6 cut(s)	430, 544, 1096, 1246, 1315, 1879
BstFNI	CGCG	1 cut(s)	1152
BstHHI	GCGC	2 cut(s)	1617, 1651
BstKTI	GATC	8 cut(s)	454, 598, 742, 802, 834, 940, 1589, 1675
BstMAI	GTCTC	3 cut(s)	699, 916, 2247
BstMBI	GATC	8 cut(s)	451, 595, 739, 799, 831, 937, 1586, 1672
BstMCI	CGRYCG	1 cut(s)	220
BstMWI	GCNNNNNNNGC	8 cut(s)	417, 776, 883, 1124, 1403, 2156, 2274, 2283
BstNI	CCWGG	5 cut(s)	181, 297, 422, 497, 1847
BstNSI	RCATGY	3 cut(s)	143, 368, 1180
BstSCI	CCNGG	7 cut(s)	179, 295, 420, 495, 1845, 2271, 2272
BstSLI	GKGCMC	1 cut(s)	1020
BstUI	CGCG	1 cut(s)	1152
BstV1I	GCAGC	4 cut(s)	429, 591, 870, 1208
BstV2I	GAAGAC	1 cut(s)	2079
BstX2I	RGATCY	4 cut(s)	595, 739, 799, 1586
BstXI	CCANNNNNNTGG	2 cut(s)	552, 796
BstYI	RGATCY	4 cut(s)	595, 739, 799, 1586
Bsu15I	ATCGAT	1 cut(s)	568
BsuI	GTATCC	1 cut(s)	503
BsuRI	GGCC	8 cut(s)	191, 420, 544, 621, 1018, 1200, 1455, 2300
BsuTUI	ATCGAT	1 cut(s)	568
BtsCI	GGATG	6 cut(s)	430, 544, 1096, 1246, 1315, 1879
BtsI	GCAGTG	1 cut(s)	879
BtsIMutI	CAGTG	7 cut(s)	102, 214, 340, 879, 1215, 1339, 1949
Cac8I	GCNNGC	5 cut(s)	225, 366, 689, 2190, 2284
CciI	TCATGA	2 cut(s)	1762, 2119
CfoI	GCGC	2 cut(s)	1617, 1651
Cfr10I	RCCGGY	3 cut(s)	470, 886, 2197
Cfr13I	GGNCC	5 cut(s)	189, 542, 1016, 1017, 2299
Cfr9I	CCCGGG	1 cut(s)	2272
ClaI	ATCGAT	1 cut(s)	568
CseI	GACGC	2 cut(s)	1379, 2257
Csp6I	GTAC	5 cut(s)	25, 201, 239, 728, 2083
CspAI	ACCGGT	2 cut(s)	470, 2197
CviQI	GTAC	5 cut(s)	25, 201, 239, 728, 2083
DdeI	CTNAG	2 cut(s)	732, 1032
DpnI	GATC	8 cut(s)	453, 597, 741, 801, 833, 939, 1588, 1674
DpnII	GATC	8 cut(s)	451, 595, 739, 799, 831, 937, 1586, 1672
DraI	TTTAAA	1 cut(s)	1042
DraIII	CACNNNGTG	1 cut(s)	2021
EaeI	YGGCCR	1 cut(s)	1198
EciI	GGCGGA	1 cut(s)	54
Ecl136II	GAGCTC	1 cut(s)	1264
Eco130I	CCWWGG	3 cut(s)	412, 823, 1439
Eco147I	AGGCCT	2 cut(s)	420, 1455
Eco24I	GRGCYC	2 cut(s)	1020, 1266
Eco31I	GGTCTC	1 cut(s)	916
Eco32I	GATATC	1 cut(s)	1842
Eco53kI	GAGCTC	1 cut(s)	1264
Eco57I	CTGAAG	2 cut(s)	1963, 2283
Eco88I	CYCGRG	1 cut(s)	2272
EcoICRI	GAGCTC	1 cut(s)	1264
EcoO109I	RGGNCCY	2 cut(s)	1016, 1017
EcoRII	CCWGG	5 cut(s)	179, 295, 420, 495, 1845
EcoRV	GATATC	1 cut(s)	1842
EcoT14I	CCWWGG	3 cut(s)	412, 823, 1439
EcoT22I	ATGCAT	1 cut(s)	145
EcoT38I	GRGCYC	2 cut(s)	1020, 1266
ErhI	CCWWGG	3 cut(s)	412, 823, 1439
FaqI	GGGAC	4 cut(s)	576, 1499, 1962, 2255
FauI	CCCGC	4 cut(s)	1217, 1583, 1814, 2197
FauNDI	CATATG	1 cut(s)	488
Fnu4HI	GCNGC	6 cut(s)	443, 580, 884, 994, 1153, 1222
FokI	GGATG	6 cut(s)	437, 551, 1103, 1233, 1322, 1886
FriOI	GRGCYC	2 cut(s)	1020, 1266
Fsp4HI	GCNGC	6 cut(s)	443, 580, 884, 994, 1153, 1222
FspBI	CTAG	3 cut(s)	81, 611, 933
FspI	TGCGCA	1 cut(s)	1650
GlaI	GCGC	2 cut(s)	1616, 1650
GluI	GCNGC	6 cut(s)	443, 580, 884, 994, 1153, 1222
GsaI	CCCAGC	1 cut(s)	1074
HaeIII	GGCC	8 cut(s)	191, 420, 544, 621, 1018, 1200, 1455, 2300
HapII	CCGG	4 cut(s)	471, 887, 2198, 2273
HgaI	GACGC	2 cut(s)	1379, 2257
HhaI	GCGC	2 cut(s)	1617, 1651
Hin1I	GRCGYC	2 cut(s)	2139, 2268
Hin6I	GCGC	2 cut(s)	1615, 1649
HinP1I	GCGC	2 cut(s)	1615, 1649
HincII	GTYRAC	3 cut(s)	331, 463, 2227
HindII	GTYRAC	3 cut(s)	331, 463, 2227
HindIII	AAGCTT	2 cut(s)	303, 1026
HinfI	GANTC	6 cut(s)	29, 269, 785, 1547, 1781, 2240
HpaI	GTTAAC	1 cut(s)	463
HpaII	CCGG	4 cut(s)	471, 887, 2198, 2273
HphI	GGTGA	3 cut(s)	485, 1556, 2008
Hpy166II	GTNNAC	8 cut(s)	137, 239, 331, 463, 1171, 1189, 1662, 2227
Hpy188I	TCNGA	5 cut(s)	173, 268, 325, 507, 1435
Hpy8I	GTNNAC	8 cut(s)	137, 239, 331, 463, 1171, 1189, 1662, 2227
Hpy99I	CGWCG	2 cut(s)	627, 2141
HpyCH4IV	ACGT	5 cut(s)	235, 811, 1185, 2114, 2139
HpyF10VI	GCNNNNNNNGC	8 cut(s)	417, 776, 883, 1124, 1403, 2156, 2274, 2283
HpyF3I	CTNAG	2 cut(s)	732, 1032
HpySE526I	ACGT	5 cut(s)	235, 811, 1185, 2114, 2139
Hsp92I	GRCGYC	2 cut(s)	2139, 2268
HspAI	GCGC	2 cut(s)	1615, 1649
KspAI	GTTAAC	1 cut(s)	463
Kzo9I	GATC	8 cut(s)	451, 595, 739, 799, 831, 937, 1586, 1672
LmnI	GCTCC	8 cut(s)	164, 439, 510, 723, 988, 1261, 1349, 1808
Lsp1109I	GCAGC	4 cut(s)	429, 591, 870, 1208
LweI	GCATC	7 cut(s)	68, 152, 565, 1114, 1393, 1693, 1851
MaeI	CTAG	3 cut(s)	81, 611, 933
MaeII	ACGT	5 cut(s)	235, 811, 1185, 2114, 2139
MaeIII	GTNAC	6 cut(s)	376, 473, 1241, 1291, 1544, 2059
MalI	GATC	8 cut(s)	453, 597, 741, 801, 833, 939, 1588, 1674
MboI	GATC	8 cut(s)	451, 595, 739, 799, 831, 937, 1586, 1672
MboII	GAAGA	6 cut(s)	96, 260, 850, 1482, 2084, 2309
MflI	RGATCY	4 cut(s)	595, 739, 799, 1586
MhlI	GDGCHC	3 cut(s)	229, 1020, 1266
MlsI	TGGCCA	1 cut(s)	1200
MluNI	TGGCCA	1 cut(s)	1200
MlyI	GAGTC	2 cut(s)	1541, 2249
MmeI	TCCRAC	1 cut(s)	1447
Mox20I	TGGCCA	1 cut(s)	1200
Mph1103I	ATGCAT	1 cut(s)	145
MroXI	GAANNNNTTC	1 cut(s)	290
MscI	TGGCCA	1 cut(s)	1200
MslI	CAYNNNNRTG	5 cut(s)	81, 116, 1110, 1308, 1701
Msp20I	TGGCCA	1 cut(s)	1200
MspA1I	CMGCKG	3 cut(s)	374, 779, 792
MspCI	CTTAAG	1 cut(s)	1525
MspI	CCGG	4 cut(s)	471, 887, 2198, 2273
MspR9I	CCNGG	7 cut(s)	181, 297, 422, 497, 1847, 2273, 2274
Mva1269I	GAATGC	1 cut(s)	2150
MvaI	CCWGG	5 cut(s)	181, 297, 422, 497, 1847
MvnI	CGCG	1 cut(s)	1152
MwoI	GCNNNNNNNGC	8 cut(s)	417, 776, 883, 1124, 1403, 2156, 2274, 2283
NciI	CCSGG	2 cut(s)	2273, 2274
NdeI	CATATG	1 cut(s)	488
NdeII	GATC	8 cut(s)	451, 595, 739, 799, 831, 937, 1586, 1672
NlaIV	GGNNCC	7 cut(s)	190, 597, 801, 1018, 1019, 1852, 2301
NmuCI	GTSAC	4 cut(s)	376, 473, 1544, 2059
NsbI	TGCGCA	1 cut(s)	1650
NsiI	ATGCAT	1 cut(s)	145
NspI	RCATGY	3 cut(s)	143, 368, 1180
NspV	TTCGAA	2 cut(s)	10, 894
OliI	CACNNNNGTG	1 cut(s)	81
PaeI	GCATGC	1 cut(s)	368
PagI	TCATGA	2 cut(s)	1762, 2119
PasI	CCCWGGG	1 cut(s)	1846
PceI	AGGCCT	2 cut(s)	420, 1455
PctI	GAATGC	1 cut(s)	2150
PdmI	GAANNNNTTC	1 cut(s)	290
PfeI	GAWTC	4 cut(s)	29, 269, 785, 1781
PfoI	TCCNGGA	1 cut(s)	495
PinAI	ACCGGT	2 cut(s)	470, 2197
PkrI	GCNGC	6 cut(s)	444, 581, 885, 995, 1154, 1223
PleI	GAGTC	2 cut(s)	1541, 2248
PpsI	GAGTC	2 cut(s)	1541, 2248
Ppu21I	YACGTR	1 cut(s)	812
PshBI	ATTAAT	1 cut(s)	1835
PsiI	TTATAA	1 cut(s)	1538
Psp124BI	GAGCTC	1 cut(s)	1266
Psp6I	CCWGG	5 cut(s)	179, 295, 420, 495, 1845
PspFI	CCCAGC	1 cut(s)	1070
PspGI	CCWGG	5 cut(s)	179, 295, 420, 495, 1845
PspN4I	GGNNCC	7 cut(s)	190, 597, 801, 1018, 1019, 1852, 2301
PspOMI	GGGCCC	1 cut(s)	1016
PspPI	GGNCC	5 cut(s)	189, 542, 1016, 1017, 2299
PsuI	RGATCY	4 cut(s)	595, 739, 799, 1586
PvuII	CAGCTG	2 cut(s)	374, 779
RsaI	GTAC	5 cut(s)	26, 202, 240, 729, 2084
RsaNI	GTAC	5 cut(s)	25, 201, 239, 728, 2083
RseI	CAYNNNNRTG	5 cut(s)	81, 116, 1110, 1308, 1701
SacI	GAGCTC	1 cut(s)	1266
SatI	GCNGC	6 cut(s)	443, 580, 884, 994, 1153, 1222
Sau3AI	GATC	8 cut(s)	451, 595, 739, 799, 831, 937, 1586, 1672
Sau96I	GGNCC	5 cut(s)	189, 542, 1016, 1017, 2299
SchI	GAGTC	2 cut(s)	1541, 2249
ScrFI	CCNGG	7 cut(s)	181, 297, 422, 497, 1847, 2273, 2274
SduI	GDGCHC	3 cut(s)	229, 1020, 1266
SfaNI	GCATC	7 cut(s)	68, 152, 565, 1114, 1393, 1693, 1851
SfuI	TTCGAA	2 cut(s)	10, 894
SmaI	CCCGGG	1 cut(s)	2274
SmiMI	CAYNNNNRTG	5 cut(s)	81, 116, 1110, 1308, 1701
SmlI	CTYRAG	4 cut(s)	228, 242, 1525, 2045
SmoI	CTYRAG	4 cut(s)	228, 242, 1525, 2045
SphI	GCATGC	1 cut(s)	368
SseBI	AGGCCT	2 cut(s)	420, 1455
SsiI	CCGC	8 cut(s)	65, 792, 994, 1152, 1224, 1576, 1807, 2190
SspMI	CTAG	3 cut(s)	81, 611, 933
SstI	GAGCTC	1 cut(s)	1266
StuI	AGGCCT	2 cut(s)	420, 1455
StyD4I	CCNGG	7 cut(s)	179, 295, 420, 495, 1845, 2271, 2272
StyI	CCWWGG	3 cut(s)	412, 823, 1439
TaiI	ACGT	5 cut(s)	238, 814, 1188, 2117, 2142
TaqI	TCGA	8 cut(s)	10, 568, 625, 853, 894, 965, 1891, 2008
TatI	WGTACW	2 cut(s)	200, 238
TauI	GCSGC	2 cut(s)	996, 1155
TfiI	GAWTC	4 cut(s)	29, 269, 785, 1781
TscAI	CASTG	7 cut(s)	102, 214, 340, 879, 1222, 1339, 1949
TseFI	GTSAC	4 cut(s)	376, 473, 1544, 2059
TseI	GCWGC	4 cut(s)	442, 579, 883, 1221
Tsp45I	GTSAC	4 cut(s)	376, 473, 1544, 2059
TspGWI	ACGGA	3 cut(s)	300, 1911, 1978
TspMI	CCCGGG	1 cut(s)	2272
TspRI	CASTG	7 cut(s)	102, 214, 340, 879, 1222, 1339, 1949
Vha464I	CTTAAG	1 cut(s)	1525
VspI	ATTAAT	1 cut(s)	1835
XapI	RAATTY	7 cut(s)	343, 627, 659, 896, 973, 1037, 2099
XceI	RCATGY	3 cut(s)	143, 368, 1180
XmaI	CCCGGG	1 cut(s)	2272
XmnI	GAANNNNTTC	1 cut(s)	290
XspI	CTAG	3 cut(s)	81, 611, 933
ZraI	GACGTC	1 cut(s)	2140
Zsp2I	ATGCAT	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.