Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0003586

Physical Location & Seq

Genomic Coordinates Reverse (-)

26718789 .. 26730310

Gene Structure 11522 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g36440.t9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2394 bp

ATGAAACTCTGCCATCATCAGAATCATAGTAGCAATCGCGAAAGGTCTAAGATGCTTCTACGTACTACCCCACATTTGTACGATTCAAATGTCTCGAGGTTCCCGCTATTTGGTTCAGCATCACCGATTTCAACTCAGGCTGGTGTTTCGTCGTGTGTTGTTGCTTCCCCTGTTCTTGATACTGGGTTGAAGTTGAGTAAACAAGCGACGGGGAGCAGTCAGCAAGGGCTTGTGAGAAAGACCCGGGATCATGTCAAGCATAGCACGGCTAATGCTTACGGTCAGGGTGCTCCAGGAAGTGTGCTAGAGGAAGAAGCGCAACTGCCAGAAACCAGCCAGCCATGGGAAACTTCTCCAGGTCAGGCTTCTCCGCTCGGTGTATCAGAAGTTGATCGTGGGGTCAATTTTGCCATTTTCTCACAGCACGCAACCGCTGTCACTCTCTGCATTAAAGTTGCAGATAGGGGACAGTTTGGAGGGCCAGATGGTGGAATGACTGAGCTGGCTCTGGATCCTAATGAGAACAAGACAGGTGACATTTGGCACATATGTATAAAGGATTTTCCCCGAAGCAATGTCCTTTATGGTTACCGTATTGATGGGCCTCATGGTTGGGATCAAGGGCATCGGTTTGACAGCAGCATTGTACTAATAGATCCTTATGCGAAGCTAGTTGAAGGTCGACGCTGTTTTGGAGATTCCAGAGAAAAGTTATCTGGATTCCTTGGAACTTACGATTTTGATAGCTCACCTTTTGATTGGGGAGACAACTACAAGCTTCCAAATATTCCTGAGAATGATCTTGTTATATATGAAATGAATGTTCGTGCATTTACAGCTGATGAATCCAGTGGTCTGGATCCAAATGTTCGTGGTAGCTACCGTGGTCTGATTGATAAGATTCCACACCTTCTAGAGCTCGGCATCAATGCTGTAGAGTTGCTGCCTGTCTTTGAATTTGATGAATTTGAGTTTCAAAGGCGTTCAAACCCTAGAGATCACATGATTAATACATGGGGTTACTCAACAATAAACTTCTTTGCGCCTATGAGCCGTTATGCGAGTGCTGGTGGAGGACCCCTTAAAGCTTCCCATGAAATTAAAGAAATGGTTAAAGCCTTGCATGGTGCTGGGATTGAGGTCATTTTGGATGTTGTCTATAACCATACAAATGAAGCTGATGACGCATACCCTTATACCACTTCATTTCGTGGCATAGATAATAAGGTTTATTACATGCTGGACCCAAACAACAATGGCCAATTACTGAACTTCTCGGGCTGTGGGAACACATTAAACTGTAACCATCCTGTTGTTATGGAGCTCGTTCTTGAGAGCTTAAGACACTGGGTGACTGAATATCATGTGGATGGATTTAGATTTGACCTTGCTAGTGTTCTTTGTCGGGGAACAAATGGAGCTCCACTTGATGCCCCGCCAGTAATCAGGGCTATCGCAAAAGATGCCATTCTATCAAGATGTAAAATTATTTCTGAGCCTTGGGATTGTGGAGGCCTTTATCTTGTTGGAAGATTTCCAAACTGGGACAGGTGGGCCGAATGGAATGGAATGTATCGTGATGACTTGAGGAGATTTATAAAGGGGGATTCGGGTATGAAAGGAAGTTTTGCAACTCGTGTTTCTGGATCTGCTGACCTTTACAAAACCAATAAGCGCAAGCCTTATCACAGTATTAATTTTATCATTGCGCATGATGGGTTCACACTATATGATCTCGTTTCATACAATTTCAAGCACAATGATGCTAATGGAGAAGGAGGAAATGATGGAAACAATGATAATCTGAGCTGGAATTGTGGTTATGAAGGAGAAACTGATGATTCTACTATTAAAGCTATACGCTCTCGGCAAATGAAAAACTTTCATCTGGCATTAATGATCTCTCAGGGAACACCAATGATGCTGATGGGAGATGAATATGGGCATACTAGATATGGAAATAACAACAGTTATGGACACGATACTGCTATTAACCATTTCCAGTGGGGACAGTTGGAATCACGGAAGAATAATCACTTCAGGTTTTTCAAGGAGGTGATAAACTATCGAAAAACACATCATGTGTTTGGTCGTGATACATTTCTTGAGAAAAGTGATGTGACATGGCATGAAGGCAACTGGGACAACTATGAGAGCAAGTTTCTTGCATTTACGTTTCATGACAATAATGGAGGAGACATCTATATAGCGTTCAATGCTCATGATTATTTTATTGAAGTTCCAATCCCCGCGCCACCAGGAACGAGGAAGTGGTTTCGTGTGGTCGACACTAATCTTGAGTCTCCCAATGACTGTGTGCTTGATGGTGTCCCAGGCATTGGAGGCACTTACAACATGGCTCCTTACTCATCAATTCTTCTTGAAGCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009657 GO:0009660 GO:0009987 GO:0015980 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0071840 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

798

Amino Acids

89.42

Weight (kDa)

5.86

Isoelectric Point (pI)

30.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	124 - 222	9.6e-12	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	288 - 416	1.5e-09	Alpha amylase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09020
fragaria_vesca	FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440
malus_domestica	MD08G1188900.v1.1 MD15G1376800.v1.1
prunus_persica	Prupe.1G523500_v2.0.a1 Prupe.1G523500_v2.0.a1
pyrus_communis	pycom08g16260 pycom15g33660
rosa_chinensis	RchiOBHm_Chr7g0238421
rosa_laevigata	RLG00000000911
rosa_multiflora	Rmu_sc0031277.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223020
rosa_rugosa	Rorug07G0312600
rosa_samantha	Rh7AG467500 Rh7BG438200 Rh7CG485500 Rh7DG454000
rosa_wichuraiana	Rw7G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1598
Acc16I	TGCGCA	1 cut(s)	1710
AccB7I	CCANNNNNTGG	2 cut(s)	488, 2339
AccBSI	CCGCTC	1 cut(s)	373
AccI	GTMKAC	2 cut(s)	682, 2286
AccII	CGCG	2 cut(s)	39, 2252
AciI	CCGC	5 cut(s)	104, 371, 432, 1436, 2250
AclWI	GGATC	8 cut(s)	255, 506, 519, 624, 650, 854, 867, 1654
AcoI	YGGCCR	1 cut(s)	1258
AcsI	RAATTY	2 cut(s)	956, 965
AcuI	CTGAAG	1 cut(s)	2023
AdeI	CACNNNGTG	1 cut(s)	1351
AfaI	GTAC	3 cut(s)	64, 80, 648
AfiI	CCNNNNNNNGG	4 cut(s)	110, 343, 488, 2339
AflII	CTTAAG	1 cut(s)	1339
AjnI	CCWGG	4 cut(s)	292, 355, 2257, 2332
Alw21I	GWGCWC	4 cut(s)	292, 921, 1326, 1423
Alw26I	GTCTC	4 cut(s)	97, 759, 2190, 2307
AlwI	GGATC	8 cut(s)	255, 506, 519, 624, 650, 854, 867, 1654
Ama87I	CYCGRG	3 cut(s)	94, 243, 1276
AoxI	GGCC	5 cut(s)	479, 602, 1258, 1513, 1554
ApeKI	GCWGC	2 cut(s)	639, 943
ApoI	RAATTY	2 cut(s)	956, 965
AseI	ATTAAT	3 cut(s)	1008, 1695, 1895
AspLEI	GCGC	5 cut(s)	319, 1045, 1677, 1711, 2254
AspS9I	GGNCC	5 cut(s)	479, 602, 1076, 1243, 1554
AsuC2I	CCSGG	2 cut(s)	244, 245
AsuHPI	GGTGA	5 cut(s)	114, 545, 741, 1363, 2068
AvaI	CYCGRG	3 cut(s)	94, 243, 1276
AvaII	GGWCC	2 cut(s)	1076, 1243
BalI	TGGCCA	1 cut(s)	1260
BamHI	GGATCC	2 cut(s)	511, 859
BanII	GRGCYC	3 cut(s)	921, 1326, 1423
BauI	CACGAG	1 cut(s)	1635
Bbv12I	GWGCWC	4 cut(s)	292, 921, 1326, 1423
BbvI	GCAGC	2 cut(s)	651, 930
BccI	CCATC	9 cut(s)	21, 479, 593, 1314, 1364, 1709, 1781, 1921, 2318
BceAI	ACGGC	2 cut(s)	282, 1038
BciT130I	CCWGG	4 cut(s)	294, 357, 2259, 2334
BcnI	CCSGG	2 cut(s)	244, 245
BcoDI	GTCTC	4 cut(s)	97, 759, 2190, 2307
BfaI	CTAG	6 cut(s)	305, 671, 914, 993, 1392, 1950
BfmI	CTRYAG	1 cut(s)	933
BfrI	CTTAAG	1 cut(s)	1339
BisI	GCNGC	2 cut(s)	640, 944
BlsI	GCNGC	2 cut(s)	641, 945
Bme1390I	CCNGG	6 cut(s)	244, 245, 294, 357, 2259, 2334
Bme18I	GGWCC	2 cut(s)	1076, 1243
BmeT110I	CYCGRG	3 cut(s)	94, 243, 1276
BmgT120I	GGNCC	5 cut(s)	479, 602, 1076, 1243, 1554
BmiI	GGNNCC	6 cut(s)	101, 513, 861, 1078, 1245, 2361
BmrFI	CCNGG	6 cut(s)	244, 245, 294, 357, 2259, 2334
BmrI	ACTGGG	4 cut(s)	192, 1357, 1552, 2149
BmsI	GCATC	8 cut(s)	42, 128, 634, 933, 1420, 1453, 1753, 1911
BmuI	ACTGGG	4 cut(s)	192, 1357, 1552, 2149
BpmI	CTGGAG	2 cut(s)	276, 339
BpuEI	CTTGAG	4 cut(s)	1352, 1606, 2126, 2318
BpuMI	CCSGG	2 cut(s)	244, 245
BsaAI	YACGTR	1 cut(s)	62
BsaJI	CCNNGG	6 cut(s)	243, 341, 724, 883, 1499, 2332
Bsc4I	CCNNNNNNNGG	4 cut(s)	110, 343, 488, 2339
Bse1I	ACTGG	7 cut(s)	187, 849, 1352, 1439, 1547, 2002, 2144
Bse3DI	GCAATG	2 cut(s)	580, 1704
BseBI	CCWGG	4 cut(s)	294, 357, 2259, 2334
BseDI	CCNNGG	6 cut(s)	243, 341, 724, 883, 1499, 2332
BseGI	GGATG	3 cut(s)	1156, 1306, 1375
BseLI	CCNNNNNNNGG	4 cut(s)	110, 343, 488, 2339
BseMI	GCAATG	2 cut(s)	580, 1704
BseMII	CTCAG	6 cut(s)	149, 489, 783, 1485, 1796, 1919
BseNI	ACTGG	7 cut(s)	187, 849, 1352, 1439, 1547, 2002, 2144
BseRI	GAGGAG	2 cut(s)	1603, 2208
BseXI	GCAGC	2 cut(s)	651, 930
BseYI	CCCAGC	1 cut(s)	1130
Bsh1236I	CGCG	2 cut(s)	39, 2252
BshFI	GGCC	5 cut(s)	481, 604, 1260, 1515, 1556
BsiHKAI	GWGCWC	4 cut(s)	292, 921, 1326, 1423
BsiHKCI	CYCGRG	3 cut(s)	94, 243, 1276
BsiSI	CCGG	1 cut(s)	244
BslFI	GGGAC	5 cut(s)	480, 1559, 2022, 2156, 2315
BslI	CCNNNNNNNGG	4 cut(s)	110, 343, 488, 2339
BsmAI	GTCTC	4 cut(s)	97, 759, 2190, 2307
BsmFI	GGGAC	5 cut(s)	480, 1559, 2022, 2156, 2315
BsnI	GGCC	5 cut(s)	481, 604, 1260, 1515, 1556
BsoBI	CYCGRG	3 cut(s)	94, 243, 1276
Bsp1286I	GDGCHC	4 cut(s)	292, 921, 1326, 1423
Bsp19I	CCATGG	1 cut(s)	341
Bsp68I	TCGCGA	1 cut(s)	39
BspACI	CCGC	5 cut(s)	104, 371, 432, 1436, 2250
BspANI	GGCC	5 cut(s)	481, 604, 1260, 1515, 1556
BspCNI	CTCAG	6 cut(s)	148, 490, 784, 1486, 1797, 1918
BspFNI	CGCG	2 cut(s)	39, 2252
BspHI	TCATGA	2 cut(s)	2179, 2221
BspLI	GGNNCC	6 cut(s)	101, 513, 861, 1078, 1245, 2361
BspPI	GGATC	8 cut(s)	255, 506, 519, 624, 650, 854, 867, 1654
BspTI	CTTAAG	1 cut(s)	1339
BsrBI	CCGCTC	1 cut(s)	373
BsrDI	GCAATG	2 cut(s)	580, 1704
BsrI	ACTGG	7 cut(s)	187, 849, 1352, 1439, 1547, 2002, 2144
BssECI	CCNNGG	6 cut(s)	243, 341, 724, 883, 1499, 2332
BssSI	CACGAG	1 cut(s)	1635
BssT1I	CCWWGG	3 cut(s)	341, 724, 1499
Bst2BI	CACGAG	1 cut(s)	1635
Bst2UI	CCWGG	4 cut(s)	294, 357, 2259, 2334
Bst4CI	ACNGT	9 cut(s)	281, 471, 593, 884, 1301, 1691, 1970, 2013, 2315
BstAFI	CTTAAG	1 cut(s)	1339
BstAPI	GCANNNNNTGC	1 cut(s)	1463
BstBAI	YACGTR	1 cut(s)	62
BstC8I	GCNNGC	4 cut(s)	338, 426, 504, 1679
BstDEI	CTNAG	7 cut(s)	48, 135, 498, 792, 1494, 1805, 1905
BstDSI	CCRYGG	2 cut(s)	341, 883
BstEII	GGTNACC	1 cut(s)	587
BstF5I	GGATG	3 cut(s)	1156, 1306, 1375
BstFNI	CGCG	2 cut(s)	39, 2252
BstHHI	GCGC	5 cut(s)	319, 1045, 1677, 1711, 2254
BstMAI	GTCTC	4 cut(s)	97, 759, 2190, 2307
BstMWI	GCNNNNNNNGC	5 cut(s)	836, 1184, 1463, 2216, 2343
BstNI	CCWGG	4 cut(s)	294, 357, 2259, 2334
BstNSI	RCATGY	1 cut(s)	1240
BstPI	GGTNACC	1 cut(s)	587
BstSCI	CCNGG	6 cut(s)	242, 243, 292, 355, 2257, 2332
BstSFI	CTRYAG	1 cut(s)	933
BstSNI	TACGTA	1 cut(s)	62
BstUI	CGCG	2 cut(s)	39, 2252
BstV1I	GCAGC	2 cut(s)	651, 930
BstX2I	RGATCY	4 cut(s)	511, 655, 859, 1646
BstXI	CCANNNNNNTGG	1 cut(s)	856
BstYI	RGATCY	4 cut(s)	511, 655, 859, 1646
BsuRI	GGCC	5 cut(s)	481, 604, 1260, 1515, 1556
BtgI	CCRYGG	2 cut(s)	341, 883
BtsCI	GGATG	3 cut(s)	1156, 1306, 1375
BtsIMutI	CAGTG	3 cut(s)	856, 1345, 2009
BtuMI	TCGCGA	1 cut(s)	39
Cac8I	GCNNGC	4 cut(s)	338, 426, 504, 1679
CciI	TCATGA	2 cut(s)	2179, 2221
CfoI	GCGC	5 cut(s)	319, 1045, 1677, 1711, 2254
Cfr13I	GGNCC	5 cut(s)	479, 602, 1076, 1243, 1554
Cfr9I	CCCGGG	1 cut(s)	243
CseI	GACGC	2 cut(s)	693, 1193
Csp6I	GTAC	3 cut(s)	63, 79, 647
CviQI	GTAC	3 cut(s)	63, 79, 647
DdeI	CTNAG	7 cut(s)	48, 135, 498, 792, 1494, 1805, 1905
DraIII	CACNNNGTG	1 cut(s)	1351
EaeI	YGGCCR	1 cut(s)	1258
Ecl136II	GAGCTC	3 cut(s)	919, 1324, 1421
Eco105I	TACGTA	1 cut(s)	62
Eco130I	CCWWGG	3 cut(s)	341, 724, 1499
Eco147I	AGGCCT	1 cut(s)	1515
Eco24I	GRGCYC	3 cut(s)	921, 1326, 1423
Eco47I	GGWCC	2 cut(s)	1076, 1243
Eco53kI	GAGCTC	3 cut(s)	919, 1324, 1421
Eco57I	CTGAAG	1 cut(s)	2023
Eco88I	CYCGRG	3 cut(s)	94, 243, 1276
Eco91I	GGTNACC	1 cut(s)	587
EcoICRI	GAGCTC	3 cut(s)	919, 1324, 1421
EcoO109I	RGGNCCY	1 cut(s)	1076
EcoO65I	GGTNACC	1 cut(s)	587
EcoRII	CCWGG	4 cut(s)	292, 355, 2257, 2332
EcoT14I	CCWWGG	3 cut(s)	341, 724, 1499
EcoT38I	GRGCYC	3 cut(s)	921, 1326, 1423
ErhI	CCWWGG	3 cut(s)	341, 724, 1499
FaqI	GGGAC	5 cut(s)	480, 1559, 2022, 2156, 2315
FauI	CCCGC	3 cut(s)	111, 1443, 2257
FauNDI	CATATG	1 cut(s)	548
FblI	GTMKAC	2 cut(s)	682, 2286
Fnu4HI	GCNGC	2 cut(s)	640, 944
FokI	GGATG	3 cut(s)	1163, 1293, 1382
FriOI	GRGCYC	3 cut(s)	921, 1326, 1423
Fsp4HI	GCNGC	2 cut(s)	640, 944
FspBI	CTAG	6 cut(s)	305, 671, 914, 993, 1392, 1950
FspI	TGCGCA	1 cut(s)	1710
GlaI	GCGC	5 cut(s)	318, 1044, 1676, 1710, 2253
GluI	GCNGC	2 cut(s)	640, 944
GsaI	CCCAGC	1 cut(s)	1134
GsuI	CTGGAG	2 cut(s)	276, 339
HaeIII	GGCC	5 cut(s)	481, 604, 1260, 1515, 1556
HapII	CCGG	1 cut(s)	244
HgaI	GACGC	2 cut(s)	693, 1193
HhaI	GCGC	5 cut(s)	319, 1045, 1677, 1711, 2254
Hin6I	GCGC	5 cut(s)	317, 1043, 1675, 1709, 2252
HinP1I	GCGC	5 cut(s)	317, 1043, 1675, 1709, 2252
HincII	GTYRAC	2 cut(s)	683, 2287
HindII	GTYRAC	2 cut(s)	683, 2287
HindIII	AAGCTT	2 cut(s)	776, 1086
HpaII	CCGG	1 cut(s)	244
HphI	GGTGA	5 cut(s)	114, 545, 741, 1363, 2068
Hpy166II	GTNNAC	4 cut(s)	200, 683, 1722, 2287
Hpy188I	TCNGA	5 cut(s)	21, 385, 891, 1495, 1806
Hpy8I	GTNNAC	4 cut(s)	200, 683, 1722, 2287
Hpy99I	CGWCG	3 cut(s)	154, 211, 687
HpyAV	CCTTC	5 cut(s)	671, 920, 1769, 1820, 2126
HpyCH4III	ACNGT	9 cut(s)	281, 471, 593, 884, 1301, 1691, 1970, 2013, 2315
HpyCH4IV	ACGT	2 cut(s)	61, 2174
HpyCH4V	TGCA	6 cut(s)	447, 458, 830, 1123, 1631, 2168
HpyF10VI	GCNNNNNNNGC	5 cut(s)	836, 1184, 1463, 2216, 2343
HpyF3I	CTNAG	7 cut(s)	48, 135, 498, 792, 1494, 1805, 1905
HpySE526I	ACGT	2 cut(s)	61, 2174
HspAI	GCGC	5 cut(s)	317, 1043, 1675, 1709, 2252
LmnI	GCTCC	6 cut(s)	213, 295, 1321, 1418, 1426, 2365
Lsp1109I	GCAGC	2 cut(s)	651, 930
LweI	GCATC	8 cut(s)	42, 128, 634, 933, 1420, 1453, 1753, 1911
MaeI	CTAG	6 cut(s)	305, 671, 914, 993, 1392, 1950
MaeII	ACGT	2 cut(s)	61, 2174
MaeIII	GTNAC	7 cut(s)	436, 533, 587, 1019, 1301, 1351, 2119
MbiI	CCGCTC	1 cut(s)	373
MboII	GAAGA	4 cut(s)	323, 1542, 2038, 2369
MflI	RGATCY	4 cut(s)	511, 655, 859, 1646
MhlI	GDGCHC	4 cut(s)	292, 921, 1326, 1423
MlsI	TGGCCA	1 cut(s)	1260
MluNI	TGGCCA	1 cut(s)	1260
MlyI	GAGTC	1 cut(s)	2309
MmeI	TCCRAC	2 cut(s)	1507, 1995
Mox20I	TGGCCA	1 cut(s)	1260
MscI	TGGCCA	1 cut(s)	1260
MslI	CAYNNNNRTG	3 cut(s)	1170, 1368, 1761
Msp20I	TGGCCA	1 cut(s)	1260
MspA1I	CMGCKG	2 cut(s)	434, 839
MspCI	CTTAAG	1 cut(s)	1339
MspI	CCGG	1 cut(s)	244
MspR9I	CCNGG	6 cut(s)	244, 245, 294, 357, 2259, 2334
MvaI	CCWGG	4 cut(s)	294, 357, 2259, 2334
MvnI	CGCG	2 cut(s)	39, 2252
MwoI	GCNNNNNNNGC	5 cut(s)	836, 1184, 1463, 2216, 2343
NciI	CCSGG	2 cut(s)	244, 245
NcoI	CCATGG	1 cut(s)	341
NdeI	CATATG	1 cut(s)	548
NlaIV	GGNNCC	6 cut(s)	101, 513, 861, 1078, 1245, 2361
NmeAIII	GCCGAG	2 cut(s)	900, 1846
NmuCI	GTSAC	4 cut(s)	436, 533, 1351, 2119
NruI	TCGCGA	1 cut(s)	39
NsbI	TGCGCA	1 cut(s)	1710
NspI	RCATGY	1 cut(s)	1240
PaeR7I	CTCGAG	1 cut(s)	94
PagI	TCATGA	2 cut(s)	2179, 2221
PceI	AGGCCT	1 cut(s)	1515
PfeI	GAWTC	9 cut(s)	22, 83, 698, 720, 845, 901, 1607, 1841, 2018
PflMI	CCANNNNNTGG	2 cut(s)	488, 2339
PfoI	TCCNGGA	1 cut(s)	292
PkrI	GCNGC	2 cut(s)	641, 945
PleI	GAGTC	1 cut(s)	2308
PpsI	GAGTC	1 cut(s)	2308
Ppu21I	YACGTR	1 cut(s)	62
PpuMI	RGGWCCY	1 cut(s)	1076
PshBI	ATTAAT	3 cut(s)	1008, 1695, 1895
PsiI	TTATAA	1 cut(s)	1598
Psp124BI	GAGCTC	3 cut(s)	921, 1326, 1423
Psp5II	RGGWCCY	1 cut(s)	1076
Psp6I	CCWGG	4 cut(s)	292, 355, 2257, 2332
PspEI	GGTNACC	1 cut(s)	587
PspFI	CCCAGC	1 cut(s)	1130
PspGI	CCWGG	4 cut(s)	292, 355, 2257, 2332
PspN4I	GGNNCC	6 cut(s)	101, 513, 861, 1078, 1245, 2361
PspPI	GGNCC	5 cut(s)	479, 602, 1076, 1243, 1554
PspPPI	RGGWCCY	1 cut(s)	1076
PsuI	RGATCY	4 cut(s)	511, 655, 859, 1646
PvuII	CAGCTG	1 cut(s)	839
RruI	TCGCGA	1 cut(s)	39
RsaI	GTAC	3 cut(s)	64, 80, 648
RsaNI	GTAC	3 cut(s)	63, 79, 647
RseI	CAYNNNNRTG	3 cut(s)	1170, 1368, 1761
SacI	GAGCTC	3 cut(s)	921, 1326, 1423
SalI	GTCGAC	2 cut(s)	681, 2285
SatI	GCNGC	2 cut(s)	640, 944
Sau96I	GGNCC	5 cut(s)	479, 602, 1076, 1243, 1554
SchI	GAGTC	1 cut(s)	2309
ScrFI	CCNGG	6 cut(s)	244, 245, 294, 357, 2259, 2334
SduI	GDGCHC	4 cut(s)	292, 921, 1326, 1423
SfaNI	GCATC	8 cut(s)	42, 128, 634, 933, 1420, 1453, 1753, 1911
SfcI	CTRYAG	1 cut(s)	933
Sfr274I	CTCGAG	1 cut(s)	94
SinI	GGWCC	2 cut(s)	1076, 1243
SlaI	CTCGAG	1 cut(s)	94
SmaI	CCCGGG	1 cut(s)	245
SmiMI	CAYNNNNRTG	3 cut(s)	1170, 1368, 1761
SmlI	CTYRAG	6 cut(s)	94, 1331, 1339, 1585, 2105, 2297
SmoI	CTYRAG	6 cut(s)	94, 1331, 1339, 1585, 2105, 2297
SnaBI	TACGTA	1 cut(s)	62
SseBI	AGGCCT	1 cut(s)	1515
SsiI	CCGC	5 cut(s)	104, 371, 432, 1436, 2250
SspI	AATATT	1 cut(s)	787
SspMI	CTAG	6 cut(s)	305, 671, 914, 993, 1392, 1950
SstI	GAGCTC	3 cut(s)	921, 1326, 1423
StuI	AGGCCT	1 cut(s)	1515
StyD4I	CCNGG	6 cut(s)	242, 243, 292, 355, 2257, 2332
StyI	CCWWGG	3 cut(s)	341, 724, 1499
TaaI	ACNGT	9 cut(s)	281, 471, 593, 884, 1301, 1691, 1970, 2013, 2315
TaiI	ACGT	2 cut(s)	64, 2177
TaqI	TCGA	4 cut(s)	95, 682, 2068, 2286
TatI	WGTACW	1 cut(s)	646
TfiI	GAWTC	9 cut(s)	22, 83, 698, 720, 845, 901, 1607, 1841, 2018
TscAI	CASTG	3 cut(s)	856, 1352, 2009
TseFI	GTSAC	4 cut(s)	436, 533, 1351, 2119
TseI	GCWGC	2 cut(s)	639, 943
Tsp45I	GTSAC	4 cut(s)	436, 533, 1351, 2119
TspGWI	ACGGA	1 cut(s)	2038
TspMI	CCCGGG	1 cut(s)	243
TspRI	CASTG	3 cut(s)	856, 1352, 2009
Van91I	CCANNNNNTGG	2 cut(s)	488, 2339
Vha464I	CTTAAG	1 cut(s)	1339
VpaK11BI	GGWCC	2 cut(s)	1076, 1243
VspI	ATTAAT	3 cut(s)	1008, 1695, 1895
XapI	RAATTY	2 cut(s)	956, 965
XbaI	TCTAGA	1 cut(s)	913
XceI	RCATGY	1 cut(s)	1240
XhoI	CTCGAG	1 cut(s)	94
XmaI	CCCGGG	1 cut(s)	243
XmiI	GTMKAC	2 cut(s)	682, 2286
XspI	CTAG	6 cut(s)	305, 671, 914, 993, 1392, 1950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.