AT4G10020

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013147
Physical Location & Seq
Forward (+)
6268273 .. 6270434
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10020.1

Sequence Viewer

Length: 1170 bp
ATGGTGGATCTATTGAACTCGGTGATGAACCTGGTGGCGCCTCCAGCGACCATGGTGGTGATGGCCTTTGCATGGCCATTACTGTCTTTCATTAGCTTCTCCGAACGGGCTTACAACTCTTATTTCGCCACCGAAAATATGGAAGATAAAGTAGTTGTCATCACCGGAGCTTCATCGGCCATTGGAGAGCAAATAGCATATGAATATGCAAAAAGAGGAGCGAATTTGGTGTTGGTGGCGAGGAGAGAGCAGAGACTGAGAGTTGTGAGTAATAAGGCTAAACAGATTGGAGCCAACCATGTGATCATCATCGCTGCTGATGTCATCAAAGAAGATGACTGCCGCCGTTTTATCACCCAAGCCGTCAACTATTACGGCCGCGTGGATCATCTAGTGAATACAGCGAGTCTTGGACACACTTTTTACTTTGAGGAAGTGAGTGACACGACTGTGTTTCCACATTTGCTGGACATAAACTTCTGGGGGAATGTTTATCCGACATACGTAGCGTTGCCATACCTTCACCAGACGAATGGCCGAATAGTCGTGAATGCATCGGTTGAAAACTGGTTGCCTCTACCACGGATGAGTCTTTATTCTGCTGCAAAAGCAGCATTAGTCAACTTCTATGAGACGCTGCGTTTCGAGCTAAATGGAGACGTTGGTATAACTATCGCGACTCACGGGTGGATTGGCAGTGAGATGAGTGGAGGAAAGTTCATGCTAGAAGAAGGTGCTGAGATGCAATGGAAGGAAGAGAGAGAAGTACCTGCAAATGGTGGACCGCTAGAGGAATTTGCAAAGATGATTGTGGCAGGAGCTTGTAGGGGAGATGCATATGTGAAGTTTCCAAACTGGTACGATGTCTTTCTCCTCTATCGAGTCTTCACACCGAATGTACTGAGATGGACATTCAAGTTGTTACTGTCTACTGAGGGTACACGTAGAAGCTCCCTTGTTGGGGTCGGGTCAGGTATGCCTGTGGATGAATCCTCTTCACAAATGAAACTTATGCTTGAAGGAGGACCACCTCGAGTTCCTGCAAGCCCACCTAGGTATACCGCAAGCCCACCTCATTATACCGCAAGCCCACCACGGTATCCTGCAAGCCCACCTCGGTATCCTGCGAGCCCACCTCGGTTTTCACAGTTTAATATCCAAGAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

389

Amino Acids

43.44

Weight (kDa)

6.41

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 50 - 236 3.3e-38 short chain dehydrogenase
KR PF08659 52 - 150 7.7e-07 KR domain
adh_short_C2 PF13561 56 - 235 7.1e-22 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 778
AccB1I GGYRCC 1 cut(s) 37
AccI GTMKAC 2 cut(s) 929, 1058
AccII CGCG 2 cut(s) 381, 677
AciI CCGC 5 cut(s) 343, 379, 785, 1062, 1083
AclWI GGATC 2 cut(s) 15, 393
AcoI YGGCCR 4 cut(s) 74, 177, 376, 535
AcsI RAATTY 2 cut(s) 223, 794
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 4 cut(s) 768, 860, 900, 940
AfiI CCNNNNNNNGG 4 cut(s) 72, 776, 960, 961
AflIII ACRYGT 1 cut(s) 941
AgsI TTSAA 4 cut(s) 16, 563, 916, 1019
AjnI CCWGG 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
AleI CACNNNNGTG 1 cut(s) 449
AluBI AGCT 5 cut(s) 96, 170, 649, 821, 951
AluI AGCT 5 cut(s) 96, 170, 649, 821, 951
Alw26I GTCTC 3 cut(s) 247, 626, 651
AlwI GGATC 2 cut(s) 15, 393
AlwNI CAGNNNCTG 1 cut(s) 256
Ama87I CYCGRG 1 cut(s) 1032
AoxI GGCC 5 cut(s) 63, 74, 177, 376, 535
ApeKI GCWGC 4 cut(s) 314, 602, 611, 637
ApoI RAATTY 2 cut(s) 223, 794
AspA2I CCTAGG 1 cut(s) 1052
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 782, 1025
AsuHPI GGTGA 5 cut(s) 34, 70, 154, 346, 515
AvaI CYCGRG 1 cut(s) 1032
AvaII GGWCC 2 cut(s) 782, 1025
AvrII CCTAGG 1 cut(s) 1052
BaeI ACNNNNGTAYC 4 cut(s) 1082, 1103, 1115, 1136
BalI TGGCCA 1 cut(s) 76
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 1133
BbsI GAAGAC 1 cut(s) 877
BbvI GCAGC 4 cut(s) 301, 589, 623, 624
BccI CCATC 2 cut(s) 55, 900
BceAI ACGGC 3 cut(s) 330, 347, 391
BciT130I CCWGG 1 cut(s) 32
BciVI GTATCC 2 cut(s) 1110, 1131
BclI TGATCA 1 cut(s) 303
BcoDI GTCTC 3 cut(s) 247, 626, 651
BfaI CTAG 4 cut(s) 392, 725, 788, 1053
BfoI RGCGCY 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 778
BfuI GTATCC 2 cut(s) 1110, 1131
BisI GCNGC 6 cut(s) 315, 343, 379, 603, 612, 638
BlnI CCTAGG 1 cut(s) 1052
BlsI GCNGC 6 cut(s) 316, 344, 380, 604, 613, 639
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 2 cut(s) 782, 1025
BmeT110I CYCGRG 1 cut(s) 1032
BmgT120I GGNCC 2 cut(s) 782, 1025
BmiI GGNNCC 2 cut(s) 39, 292
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 3 cut(s) 563, 732, 823
BpiI GAAGAC 1 cut(s) 877
BplI GAGNNNNNCTC 2 cut(s) 1120, 1152
BpmI CTGGAG 1 cut(s) 27
BsaAI YACGTR 2 cut(s) 505, 944
BsaBI GATNNNNATC 1 cut(s) 308
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 6 cut(s) 51, 581, 1052, 1094, 1115, 1136
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 776, 960, 961
Bse1I ACTGG 2 cut(s) 572, 860
Bse3DI GCAATG 1 cut(s) 752
Bse8I GATNNNNATC 1 cut(s) 308
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 6 cut(s) 51, 581, 1052, 1094, 1115, 1136
BseGI GGATG 2 cut(s) 591, 991
BseJI GATNNNNATC 1 cut(s) 308
BseLI CCNNNNNNNGG 4 cut(s) 72, 776, 960, 961
BseMI GCAATG 1 cut(s) 752
BseMII CTCAG 4 cut(s) 248, 729, 893, 924
BseNI ACTGG 2 cut(s) 572, 860
BseRI GAGGAG 3 cut(s) 231, 256, 863
BseX3I CGGCCG 1 cut(s) 376
BseXI GCAGC 4 cut(s) 301, 589, 623, 624
Bsh1236I CGCG 2 cut(s) 381, 677
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 5 cut(s) 65, 76, 179, 378, 537
BshNI GGYRCC 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 1032
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 4 cut(s) 72, 776, 960, 961
BsmAI GTCTC 3 cut(s) 247, 626, 651
BsmBI CGTCTC 2 cut(s) 626, 651
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 5 cut(s) 65, 76, 179, 378, 537
BsoBI CYCGRG 1 cut(s) 1032
Bsp1286I GDGCHC 1 cut(s) 1133
Bsp143I GATC 3 cut(s) 7, 303, 385
Bsp19I CCATGG 1 cut(s) 51
Bsp68I TCGCGA 1 cut(s) 677
BspACI CCGC 5 cut(s) 343, 379, 785, 1062, 1083
BspANI GGCC 5 cut(s) 65, 76, 179, 378, 537
BspCNI CTCAG 4 cut(s) 249, 730, 894, 925
BspFNI CGCG 2 cut(s) 381, 677
BspLI GGNNCC 2 cut(s) 39, 292
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 2 cut(s) 15, 393
BspT107I GGYRCC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 752
BsrI ACTGG 2 cut(s) 572, 860
BssECI CCNNGG 6 cut(s) 51, 581, 1052, 1094, 1115, 1136
BssMI GATC 3 cut(s) 7, 303, 385
BssNAI GTATAC 1 cut(s) 1059
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 51, 1052
Bst1107I GTATAC 1 cut(s) 1059
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 5 cut(s) 84, 451, 927, 1098, 1149
Bst6I CTCTTC 2 cut(s) 750, 1000
BstACI GRCGYC 1 cut(s) 38
BstBAI YACGTR 2 cut(s) 505, 944
BstC8I GCNNGC 5 cut(s) 1045, 1066, 1087, 1108, 1129
BstDEI CTNAG 4 cut(s) 257, 738, 902, 933
BstDSI CCRYGG 3 cut(s) 51, 581, 1094
BstF5I GGATG 2 cut(s) 591, 991
BstFNI CGCG 2 cut(s) 381, 677
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 3 cut(s) 10, 306, 388
BstMAI GTCTC 3 cut(s) 247, 626, 651
BstMBI GATC 3 cut(s) 7, 303, 385
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 5 cut(s) 44, 176, 608, 611, 646
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstSNI TACGTA 1 cut(s) 505
BstUI CGCG 2 cut(s) 381, 677
BstV1I GCAGC 4 cut(s) 301, 589, 623, 624
BstV2I GAAGAC 1 cut(s) 877
BstX2I RGATCY 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 533
BstYI RGATCY 1 cut(s) 7
BstZ17I GTATAC 1 cut(s) 1059
BstZI CGGCCG 1 cut(s) 376
BsuI GTATCC 2 cut(s) 1110, 1131
BsuRI GGCC 5 cut(s) 65, 76, 179, 378, 537
BtgI CCRYGG 3 cut(s) 51, 581, 1094
BtgZI GCGATG 1 cut(s) 295
BtsCI GGATG 2 cut(s) 591, 991
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 1 cut(s) 703
BtuMI TCGCGA 1 cut(s) 677
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 5 cut(s) 1045, 1066, 1087, 1108, 1129
CaiI CAGNNNCTG 1 cut(s) 256
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 782, 1025
CseI GACGC 1 cut(s) 643
CsiI ACCWGGT 1 cut(s) 30
Csp6I GTAC 4 cut(s) 767, 859, 899, 939
CviAII CATG 4 cut(s) 52, 72, 299, 721
CviQI GTAC 4 cut(s) 767, 859, 899, 939
DdeI CTNAG 4 cut(s) 257, 738, 902, 933
DinI GGCGCC 1 cut(s) 39
DpnI GATC 3 cut(s) 9, 305, 387
DpnII GATC 3 cut(s) 7, 303, 385
EaeI YGGCCR 4 cut(s) 74, 177, 376, 535
EagI CGGCCG 1 cut(s) 376
Eam1104I CTCTTC 2 cut(s) 750, 1000
EarI CTCTTC 2 cut(s) 750, 1000
EclXI CGGCCG 1 cut(s) 376
Eco105I TACGTA 1 cut(s) 505
Eco130I CCWWGG 2 cut(s) 51, 1052
Eco24I GRGCYC 1 cut(s) 1133
Eco47I GGWCC 2 cut(s) 782, 1025
Eco52I CGGCCG 1 cut(s) 376
Eco88I CYCGRG 1 cut(s) 1032
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 2 cut(s) 51, 1052
EcoT22I ATGCAT 2 cut(s) 556, 838
EcoT38I GRGCYC 1 cut(s) 1133
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
ErhI CCWWGG 2 cut(s) 51, 1052
Esp3I CGTCTC 2 cut(s) 626, 651
FaeI CATG 4 cut(s) 55, 75, 302, 724
FatI CATG 4 cut(s) 51, 71, 298, 720
FauNDI CATATG 2 cut(s) 199, 838
FbaI TGATCA 1 cut(s) 303
FblI GTMKAC 2 cut(s) 929, 1058
Fnu4HI GCNGC 6 cut(s) 315, 343, 379, 603, 612, 638
FokI GGATG 2 cut(s) 598, 998
FriOI GRGCYC 1 cut(s) 1133
Fsp4HI GCNGC 6 cut(s) 315, 343, 379, 603, 612, 638
FspBI CTAG 4 cut(s) 392, 725, 788, 1053
GlaI GCGC 1 cut(s) 39
GluI GCNGC 6 cut(s) 315, 343, 379, 603, 612, 638
GsuI CTGGAG 1 cut(s) 27
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 5 cut(s) 65, 76, 179, 378, 537
HapII CCGG 1 cut(s) 165
HgaI GACGC 1 cut(s) 643
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 4 cut(s) 55, 75, 302, 724
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 367, 622
HindII GTYRAC 2 cut(s) 367, 622
HinfI GANTC 5 cut(s) 406, 589, 679, 882, 989
HpaII CCGG 1 cut(s) 165
HphI GGTGA 5 cut(s) 34, 70, 154, 346, 515
Hpy166II GTNNAC 6 cut(s) 367, 622, 782, 930, 941, 1059
Hpy188I TCNGA 2 cut(s) 103, 498
Hpy188III TCNNGA 2 cut(s) 547, 676
Hpy8I GTNNAC 6 cut(s) 367, 622, 782, 930, 941, 1059
HpyAV CCTTC 4 cut(s) 530, 725, 745, 1013
HpyCH4III ACNGT 5 cut(s) 84, 451, 927, 1098, 1149
HpyCH4IV ACGT 3 cut(s) 504, 660, 943
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 176, 608, 611, 646
HpyF3I CTNAG 4 cut(s) 257, 738, 902, 933
HpySE526I ACGT 3 cut(s) 504, 660, 943
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 4 cut(s) 55, 75, 302, 724
HspAI GCGC 1 cut(s) 38
KasI GGCGCC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 3 cut(s) 7, 303, 385
LmnI GCTCC 5 cut(s) 167, 218, 290, 818, 956
Lsp1109I GCAGC 4 cut(s) 301, 589, 623, 624
LweI GCATC 3 cut(s) 563, 732, 823
MabI ACCWGGT 1 cut(s) 30
MaeI CTAG 4 cut(s) 392, 725, 788, 1053
MaeII ACGT 3 cut(s) 504, 660, 943
MaeIII GTNAC 2 cut(s) 440, 921
MalI GATC 3 cut(s) 9, 305, 387
MboI GATC 3 cut(s) 7, 303, 385
MboII GAAGA 6 cut(s) 155, 344, 740, 767, 877, 987
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 1133
MlsI TGGCCA 1 cut(s) 76
MluCI AATT 2 cut(s) 223, 794
MluNI TGGCCA 1 cut(s) 76
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 4 cut(s) 415, 598, 673, 891
MmeI TCCRAC 1 cut(s) 521
Mox20I TGGCCA 1 cut(s) 76
Mph1103I ATGCAT 2 cut(s) 556, 838
MscI TGGCCA 1 cut(s) 76
MseI TTAA 1 cut(s) 1152
MslI CAYNNNNRTG 2 cut(s) 56, 449
Msp20I TGGCCA 1 cut(s) 76
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 32
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 2 cut(s) 381, 677
MwoI GCNNNNNNNGC 5 cut(s) 44, 176, 608, 611, 646
NarI GGCGCC 1 cut(s) 38
NcoI CCATGG 1 cut(s) 51
NdeI CATATG 2 cut(s) 199, 838
NdeII GATC 3 cut(s) 7, 303, 385
NlaIII CATG 4 cut(s) 55, 75, 302, 724
NlaIV GGNNCC 2 cut(s) 39, 292
NmuCI GTSAC 1 cut(s) 440
NruI TCGCGA 1 cut(s) 677
NsiI ATGCAT 2 cut(s) 556, 838
OliI CACNNNNGTG 1 cut(s) 449
PaeR7I CTCGAG 1 cut(s) 1032
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 1 cut(s) 989
PkrI GCNGC 6 cut(s) 316, 344, 380, 604, 613, 639
PleI GAGTC 4 cut(s) 414, 597, 673, 890
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 4 cut(s) 414, 597, 673, 890
Ppu21I YACGTR 2 cut(s) 505, 944
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 39, 292
PspPI GGNCC 2 cut(s) 782, 1025
PspXI VCTCGAGB 1 cut(s) 1032
PstNI CAGNNNCTG 1 cut(s) 256
PsuI RGATCY 1 cut(s) 7
RruI TCGCGA 1 cut(s) 677
RsaI GTAC 4 cut(s) 768, 860, 900, 940
RsaNI GTAC 4 cut(s) 767, 859, 899, 939
RseI CAYNNNNRTG 2 cut(s) 56, 449
SaqAI TTAA 1 cut(s) 1152
SatI GCNGC 6 cut(s) 315, 343, 379, 603, 612, 638
Sau3AI GATC 3 cut(s) 7, 303, 385
Sau96I GGNCC 2 cut(s) 782, 1025
SchI GAGTC 4 cut(s) 415, 598, 673, 891
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 1 cut(s) 1133
SexAI ACCWGGT 1 cut(s) 30
SfaNI GCATC 3 cut(s) 563, 732, 823
SfoI GGCGCC 1 cut(s) 39
Sfr274I CTCGAG 1 cut(s) 1032
SinI GGWCC 2 cut(s) 782, 1025
SlaI CTCGAG 1 cut(s) 1032
SmiMI CAYNNNNRTG 2 cut(s) 56, 449
SmlI CTYRAG 1 cut(s) 1032
SmoI CTYRAG 1 cut(s) 1032
SnaBI TACGTA 1 cut(s) 505
Sse9I AATT 2 cut(s) 223, 794
SsiI CCGC 5 cut(s) 343, 379, 785, 1062, 1083
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 4 cut(s) 392, 725, 788, 1053
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 2 cut(s) 51, 1052
TaaI ACNGT 5 cut(s) 84, 451, 927, 1098, 1149
TaiI ACGT 3 cut(s) 507, 663, 946
TaqI TCGA 3 cut(s) 645, 880, 1033
TasI AATT 2 cut(s) 223, 794
TatI WGTACW 1 cut(s) 898
TauI GCSGC 2 cut(s) 345, 381
TfiI GAWTC 1 cut(s) 989
Tru1I TTAA 1 cut(s) 1152
Tru9I TTAA 1 cut(s) 1152
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 4 cut(s) 314, 602, 611, 637
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 7 cut(s) 41, 79, 162, 216, 709, 1002, 1019
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 1 cut(s) 703
VpaK11BI GGWCC 2 cut(s) 782, 1025
XapI RAATTY 2 cut(s) 223, 794
XcmI CCANNNNNNNNNTGG 2 cut(s) 58, 136
XhoI CTCGAG 1 cut(s) 1032
XmaJI CCTAGG 1 cut(s) 1052
XmiI GTMKAC 2 cut(s) 929, 1058
XspI CTAG 4 cut(s) 392, 725, 788, 1053
Zsp2I ATGCAT 2 cut(s) 556, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.