Rw7G034750

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013147
Physical Location & Seq
Forward (+)
51623748 .. 51633379
9632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G034750.1

Sequence Viewer

Length: 1083 bp
ATGGATTTGCTTAACTCGGTTCTGAATTTCGTGGTGCCACCGGCAAGTTTGGTGATGCTGGCTTTTTCTTGGCCTGCACTGTGCTTCATCAGTGGTTGCGACGAGTGGCTTTACAACCAGTTCAATGGTGAAGATATGGAGGACAAAGTGGTTATAATTACTGGAGCTTCTTCTGGCATAGGGGAGCAAATTGCATATGAATATGCAAAGAGGCGGGCGAAGCTTGTATTAGTGGCAAGAAGAGAGACCAGGCTGCGAGGGATTGGTGAGAATGCAAGGTTCTTGGGTGCAAAGCATGTCTTGATAATTGCTGCAGATGTTGTCAAGGAGGAGGACTGTAGGAGATTCATTAATGAAACCATAAACATTTACGGCCGAGTGGATCATCTTGTAAACACAGTAAGCTTAGGACACACGTTCAGCTTTGAGGAAGTTACAGACACTTCTGTGTTTCCCCAGCTGTTGGACATAAATTTTTGGGGAAATGTTTATCCTACATATGTTGCTCTTCCTTACCTGCGTCAAACCCATGGAAAAGTCATTGTTAATGCTTCAGTTGAGAGCTGGTTACCTTTGCCGCGGATGAGTTTATATGCTGCGGCAAAGGCAGCTCTGGTCAACTTTTATGAGACACTGAGATTAGAAGTAAAAGACGACGTGGGGATAACAATTGCAACTCATGGATGGATTGTTAGTGAAATGTCAAGAGGCAAGTTCATGGTAGATGAGGGTGCTGAAATGCAATGGAAGGAAGAAAGAGAGGTACATGTGAGTGGTGGACCGGTGGAGGATTTTGCAAGGTTAATTGTAGCAGGGGCTTGCCGAGGAGATGCATACGTGAAGTATCCAAGCTGGTATGACATATTCCTGCTTTACAGGGTGTTTGCACCTAATATTCTGAATTGGACATTCCGCATGATTCTTCCTACACACGGTATGAGGAGAACTTCACTCATTGGCACGGGGAGGCCTATTTCATCTGAAGGTAGCTATGGAAGGCCTTTGTTGGAAGGAACTTCACCAAGGAGACTTCTTGTTAGTCCAACTTTCACTCCACTCAGTCATCAGCACAAACTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

360

Amino Acids

40.63

Weight (kDa)

6.33

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 49 - 236 6.2e-36 short chain dehydrogenase
adh_short_C2 PF13561 55 - 235 4.5e-20 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 155
Acc36I ACCTGC 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 34
AccB7I CCANNNNNTGG 1 cut(s) 463
AccII CGCG 1 cut(s) 580
AciI CCGC 5 cut(s) 214, 578, 580, 599, 913
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 373
AcsI RAATTY 2 cut(s) 25, 472
AcuI CTGAAG 2 cut(s) 537, 1002
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 2 cut(s) 463, 932
AflIII ACRYGT 2 cut(s) 414, 766
AgeI ACCGGT 1 cut(s) 781
AgsI TTSAA 1 cut(s) 124
AjiI CACGTC 1 cut(s) 658
AjnI CCWGG 1 cut(s) 248
AleI CACNNNNGTG 1 cut(s) 446
AluBI AGCT 9 cut(s) 167, 223, 405, 423, 460, 564, 611, 852, 990
AluI AGCT 9 cut(s) 167, 223, 405, 423, 460, 564, 611, 852, 990
Alw26I GTCTC 3 cut(s) 239, 623, 1021
AlwI GGATC 1 cut(s) 390
AoxI GGCC 4 cut(s) 71, 373, 968, 998
ApeKI GCWGC 4 cut(s) 253, 311, 596, 608
ApoI RAATTY 2 cut(s) 25, 472
AseI ATTAAT 1 cut(s) 351
AsiGI ACCGGT 1 cut(s) 781
AspS9I GGNCC 1 cut(s) 779
AsuHPI GGTGA 4 cut(s) 64, 140, 278, 1011
AvaII GGWCC 1 cut(s) 779
BanI GGYRCC 1 cut(s) 34
BbvI GCAGC 4 cut(s) 240, 298, 583, 620
BccI CCATC 1 cut(s) 678
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 1 cut(s) 250
BciVI GTATCC 1 cut(s) 855
BcoDI GTCTC 3 cut(s) 239, 623, 1021
BfmI CTRYAG 2 cut(s) 312, 337
BfuAI ACCTGC 1 cut(s) 525
BfuI GTATCC 1 cut(s) 855
BisI GCNGC 6 cut(s) 254, 312, 578, 597, 600, 609
BlsI GCNGC 6 cut(s) 255, 313, 579, 598, 601, 610
Bme1390I CCNGG 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 779
BmgBI CACGTC 1 cut(s) 658
BmgT120I GGNCC 1 cut(s) 779
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 45, 820
BpmI CTGGAG 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 838
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 4 cut(s) 529, 578, 823, 1022
BsaWI WCCGGW 1 cut(s) 781
BsaXI ACNNNNNCTCC 2 cut(s) 1036, 1066
Bsc4I CCNNNNNNNGG 2 cut(s) 463, 932
Bse118I RCCGGY 2 cut(s) 40, 781
Bse1I ACTGG 3 cut(s) 118, 166, 1080
Bse3DI GCAATG 1 cut(s) 749
BseBI CCWGG 1 cut(s) 250
BseDI CCNNGG 4 cut(s) 529, 578, 823, 1022
BseGI GGATG 2 cut(s) 588, 689
BseLI CCNNNNNNNGG 2 cut(s) 463, 932
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 2 cut(s) 626, 1072
BseNI ACTGG 3 cut(s) 118, 166, 1080
BseRI GAGGAG 3 cut(s) 344, 840, 955
BseX3I CGGCCG 1 cut(s) 373
BseXI GCAGC 4 cut(s) 240, 298, 583, 620
BseYI CCCAGC 1 cut(s) 456
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 580
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 4 cut(s) 73, 375, 970, 1000
BshNI GGYRCC 1 cut(s) 34
BshTI ACCGGT 1 cut(s) 781
BsiEI CGRYCG 1 cut(s) 376
BsiSI CCGG 2 cut(s) 41, 782
BslI CCNNNNNNNGG 2 cut(s) 463, 932
BsmAI GTCTC 3 cut(s) 239, 623, 1021
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 4 cut(s) 73, 375, 970, 1000
Bso31I GGTCTC 1 cut(s) 239
Bsp143I GATC 1 cut(s) 382
Bsp19I CCATGG 1 cut(s) 529
BspACI CCGC 5 cut(s) 214, 578, 580, 599, 913
BspANI GGCC 4 cut(s) 73, 375, 970, 1000
BspCNI CTCAG 2 cut(s) 627, 1071
BspFNI CGCG 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 36
BspMAI CTGCAG 1 cut(s) 316
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 1 cut(s) 390
BspQI GCTCTTC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 749
BsrFI RCCGGY 2 cut(s) 40, 781
BsrI ACTGG 3 cut(s) 118, 166, 1080
BssAI RCCGGY 2 cut(s) 40, 781
BssECI CCNNGG 4 cut(s) 529, 578, 823, 1022
BssMI GATC 1 cut(s) 382
BssT1I CCWWGG 2 cut(s) 529, 1022
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 4 cut(s) 81, 338, 400, 935
Bst6I CTCTTC 2 cut(s) 235, 513
BstBAI YACGTR 1 cut(s) 838
BstC8I GCNNGC 4 cut(s) 60, 75, 216, 820
BstDEI CTNAG 3 cut(s) 406, 635, 1058
BstDSI CCRYGG 2 cut(s) 529, 578
BstEII GGTNACC 1 cut(s) 567
BstF5I GGATG 2 cut(s) 588, 689
BstFNI CGCG 1 cut(s) 580
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 3 cut(s) 239, 623, 1021
BstMBI GATC 1 cut(s) 382
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 3 cut(s) 220, 605, 608
BstNI CCWGG 1 cut(s) 250
BstNSI RCATGY 2 cut(s) 299, 770
BstPI GGTNACC 1 cut(s) 567
BstSCI CCNGG 1 cut(s) 248
BstSFI CTRYAG 2 cut(s) 312, 337
BstUI CGCG 1 cut(s) 580
BstV1I GCAGC 4 cut(s) 240, 298, 583, 620
BstXI CCANNNNNNTGG 1 cut(s) 125
BstZI CGGCCG 1 cut(s) 373
BsuI GTATCC 1 cut(s) 855
BsuRI GGCC 4 cut(s) 73, 375, 970, 1000
BtgI CCRYGG 2 cut(s) 529, 578
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 2 cut(s) 588, 689
BtsIMutI CAGTG 3 cut(s) 77, 97, 632
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 4 cut(s) 60, 75, 216, 820
Cfr10I RCCGGY 2 cut(s) 40, 781
Cfr13I GGNCC 1 cut(s) 779
Cfr42I CCGCGG 1 cut(s) 581
CseI GACGC 1 cut(s) 509
Csp6I GTAC 1 cut(s) 764
CspAI ACCGGT 1 cut(s) 781
CviAII CATG 6 cut(s) 296, 530, 680, 718, 767, 916
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 3 cut(s) 406, 635, 1058
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 373
EagI CGGCCG 1 cut(s) 373
Eam1104I CTCTTC 2 cut(s) 235, 513
EarI CTCTTC 2 cut(s) 235, 513
EclXI CGGCCG 1 cut(s) 373
Eco130I CCWWGG 2 cut(s) 529, 1022
Eco147I AGGCCT 2 cut(s) 970, 1000
Eco31I GGTCTC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 779
Eco52I CGGCCG 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 537, 1002
Eco91I GGTNACC 1 cut(s) 567
EcoO65I GGTNACC 1 cut(s) 567
EcoRII CCWGG 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 529, 1022
EcoT22I ATGCAT 1 cut(s) 835
ErhI CCWWGG 2 cut(s) 529, 1022
FaeI CATG 6 cut(s) 299, 533, 683, 721, 770, 919
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FatI CATG 6 cut(s) 295, 529, 679, 717, 766, 915
FauI CCCGC 1 cut(s) 207
FauNDI CATATG 2 cut(s) 196, 499
Fnu4HI GCNGC 6 cut(s) 254, 312, 578, 597, 600, 609
FokI GGATG 2 cut(s) 595, 696
Fsp4HI GCNGC 6 cut(s) 254, 312, 578, 597, 600, 609
GluI GCNGC 6 cut(s) 254, 312, 578, 597, 600, 609
GsaI CCCAGC 1 cut(s) 460
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 4 cut(s) 73, 375, 970, 1000
HapII CCGG 2 cut(s) 41, 782
HgaI GACGC 1 cut(s) 509
Hin1II CATG 6 cut(s) 299, 533, 683, 721, 770, 919
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 2 cut(s) 221, 403
HinfI GANTC 2 cut(s) 345, 919
HpaII CCGG 2 cut(s) 41, 782
HphI GGTGA 4 cut(s) 64, 140, 278, 1011
Hpy166II GTNNAC 3 cut(s) 394, 619, 779
Hpy188I TCNGA 3 cut(s) 24, 900, 982
Hpy188III TCNNGA 2 cut(s) 301, 705
Hpy8I GTNNAC 3 cut(s) 394, 619, 779
Hpy99I CGWCG 2 cut(s) 104, 659
HpyAV CCTTC 4 cut(s) 742, 977, 990, 1004
HpyCH4III ACNGT 4 cut(s) 81, 338, 400, 935
HpyCH4IV ACGT 3 cut(s) 416, 657, 837
HpyF10VI GCNNNNNNNGC 3 cut(s) 220, 605, 608
HpyF3I CTNAG 3 cut(s) 406, 635, 1058
HpySE526I ACGT 3 cut(s) 416, 657, 837
Hsp92II CATG 6 cut(s) 299, 533, 683, 721, 770, 919
KspI CCGCGG 1 cut(s) 581
Kzo9I GATC 1 cut(s) 382
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 2 cut(s) 164, 184
Lsp1109I GCAGC 4 cut(s) 240, 298, 583, 620
LweI GCATC 2 cut(s) 45, 820
MaeII ACGT 3 cut(s) 416, 657, 837
MaeIII GTNAC 2 cut(s) 433, 567
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MboII GAAGA 6 cut(s) 143, 162, 252, 500, 764, 914
MfeI CAATTG 1 cut(s) 669
MluCI AATT 8 cut(s) 25, 156, 189, 306, 472, 669, 804, 901
MmeI TCCRAC 3 cut(s) 444, 987, 1067
Mph1103I ATGCAT 1 cut(s) 835
MseI TTAA 4 cut(s) 12, 351, 546, 803
MslI CAYNNNNRTG 2 cut(s) 446, 771
MspA1I CMGCKG 2 cut(s) 460, 580
MspI CCGG 2 cut(s) 41, 782
MspR9I CCNGG 1 cut(s) 250
MunI CAATTG 1 cut(s) 669
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 1 cut(s) 250
MvnI CGCG 1 cut(s) 580
MwoI GCNNNNNNNGC 3 cut(s) 220, 605, 608
NcoI CCATGG 1 cut(s) 529
NdeI CATATG 2 cut(s) 196, 499
NdeII GATC 1 cut(s) 382
NlaIII CATG 6 cut(s) 299, 533, 683, 721, 770, 919
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 2 cut(s) 401, 848
NsiI ATGCAT 1 cut(s) 835
NspI RCATGY 2 cut(s) 299, 770
OliI CACNNNNGTG 1 cut(s) 446
PceI AGGCCT 2 cut(s) 970, 1000
PciI ACATGT 1 cut(s) 766
PciSI GCTCTTC 1 cut(s) 513
PctI GAATGC 1 cut(s) 277
PfeI GAWTC 2 cut(s) 345, 919
PflMI CCANNNNNTGG 1 cut(s) 463
PinAI ACCGGT 1 cut(s) 781
PkrI GCNGC 6 cut(s) 255, 313, 579, 598, 601, 610
Ppu21I YACGTR 1 cut(s) 838
PscI ACATGT 1 cut(s) 766
PshBI ATTAAT 1 cut(s) 351
PsiI TTATAA 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 248
PspEI GGTNACC 1 cut(s) 567
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 779
PstI CTGCAG 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 460
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
RseI CAYNNNNRTG 2 cut(s) 446, 771
SacII CCGCGG 1 cut(s) 581
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 4 cut(s) 12, 351, 546, 803
SatI GCNGC 6 cut(s) 254, 312, 578, 597, 600, 609
Sau3AI GATC 1 cut(s) 382
Sau96I GGNCC 1 cut(s) 779
ScrFI CCNGG 1 cut(s) 250
SfaNI GCATC 2 cut(s) 45, 820
SfcI CTRYAG 2 cut(s) 312, 337
Sfr303I CCGCGG 1 cut(s) 581
SgrBI CCGCGG 1 cut(s) 581
SinI GGWCC 1 cut(s) 779
SmiMI CAYNNNNRTG 2 cut(s) 446, 771
Sse9I AATT 8 cut(s) 25, 156, 189, 306, 472, 669, 804, 901
SseBI AGGCCT 2 cut(s) 970, 1000
SsiI CCGC 5 cut(s) 214, 578, 580, 599, 913
SspI AATATT 1 cut(s) 895
StuI AGGCCT 2 cut(s) 970, 1000
StyD4I CCNGG 1 cut(s) 248
StyI CCWWGG 2 cut(s) 529, 1022
TaaI ACNGT 4 cut(s) 81, 338, 400, 935
TaiI ACGT 3 cut(s) 419, 660, 840
TasI AATT 8 cut(s) 25, 156, 189, 306, 472, 669, 804, 901
TauI GCSGC 2 cut(s) 580, 602
TfiI GAWTC 2 cut(s) 345, 919
Tru1I TTAA 4 cut(s) 12, 351, 546, 803
Tru9I TTAA 4 cut(s) 12, 351, 546, 803
TscAI CASTG 3 cut(s) 84, 97, 639
TseI GCWGC 4 cut(s) 253, 311, 596, 608
TspDTI ATGAA 6 cut(s) 76, 213, 337, 369, 706, 966
TspRI CASTG 3 cut(s) 84, 97, 639
Van91I CCANNNNNTGG 1 cut(s) 463
VpaK11BI GGWCC 1 cut(s) 779
VspI ATTAAT 1 cut(s) 351
XapI RAATTY 2 cut(s) 25, 472
XceI RCATGY 2 cut(s) 299, 770
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.