RLG00000001417

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013147
Physical Location & Seq
Reverse (-)
15346124 .. 15349543
3420 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001417

Sequence Viewer

Length: 1110 bp
ATGGATTTGTTTAACTCGGTTCTGAATTTCGTGGTGCCACCGGCAAGTTTGGTGATGCTGGCTTTTTCTTGGCCTGCACTGTGCTTCATCAGTGGTTGCGAGTGGCTTTACAACCAGTTCAATGGTGAAGATATGGAGGACAAAGTGGTTATAATTACTGGAGCTTCTTCTGGCATAGGGGAGCAAATTGCATATGAATATGCAAAGAGGAGGGCGAAGCTTGTATTAGTGGCAAGAAGAGAGACCAGGCTGCGAGGGATTGGTGAGAATGCAAGGTTCTTGGGTGCAAAGCATGTCTTGATAATTGCTGCAGATGTTGTCAAGGAGGAGGACTGTAGGAGATTCATTAATGAAACCATAAACATTTATGGCCGAGTTGATCATCTTGTAAACACAGTAAGCTTAGGACACACGTTCAGCTTTGAGGAAGTTACAGACACCTCTGTGTTTCCCCAGCTGTTGGACATAAATTTTTGGGGAAATGTTTATCCTACATATGTTGCTCTTCCTTACCTGCGTCAAACCCATGGAAAAGTCATTGTTAATGCTTCAGTTGAGAGCTGGTTACCTTTGCCGCGGATGAGTTTATATGCTGCGGCAAAGGCAGCTCTGGTCAACTTTTATGAGACACTGAGATTGGAAGTAAAAGACGACGTGGGAATAACAATTGCAACTCATGGATGGATTGTTAGTGAAATGTCGAGAGGCAAGTTCATGGTAGATGAGGGTGCTGAAATGCAATGGAAGGAAGAAAGAGAGGTACATGCAAGTGGTGGACCGGTGGAGGATTTTGCAAGGTTAATTGTGGCAGGGGCTTGCCGAGGAGATGCATATGTGAAGTATCCAAGCTGGTATGACATATTCCTGCTTTACAGGGTGTTTGCACCAAATATTCTGAATTGGACATTCCGCATGATTCTTCCTAGTCACGGTACAAGGCGAACTTCACTCATTGGCACGGGGAGGCCTCTATCATCTGAAGGTAGCTATGGGAGGTCCATTTCATATGAAGGTAGCTATGGAAGGCCTTTGTTGGAAGGAACTTCACCAACGAGACTTCTTGTTAGTCCAACTTTCACTCCACTCAGTCATCAGCACAAACTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

370

Amino Acids

41.52

Weight (kDa)

6.33

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 48 - 235 6.7e-36 short chain dehydrogenase
adh_short_C2 PF13561 54 - 236 3.8e-20 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 34
AccB7I CCANNNNNTGG 1 cut(s) 460
AccII CGCG 1 cut(s) 577
AciI CCGC 4 cut(s) 575, 577, 596, 910
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 2 cut(s) 25, 469
AcuI CTGAAG 2 cut(s) 534, 999
AfaI GTAC 2 cut(s) 762, 934
AfiI CCNNNNNNNGG 2 cut(s) 460, 929
AflIII ACRYGT 1 cut(s) 411
AgeI ACCGGT 1 cut(s) 778
AgsI TTSAA 1 cut(s) 121
AjiI CACGTC 1 cut(s) 655
AjnI CCWGG 1 cut(s) 245
AleI CACNNNNGTG 1 cut(s) 443
Alw26I GTCTC 3 cut(s) 236, 620, 1048
AoxI GGCC 4 cut(s) 71, 370, 965, 1025
ApeKI GCWGC 4 cut(s) 250, 308, 593, 605
ApoI RAATTY 2 cut(s) 25, 469
AseI ATTAAT 1 cut(s) 348
AsiGI ACCGGT 1 cut(s) 778
AspS9I GGNCC 2 cut(s) 776, 996
AsuHPI GGTGA 4 cut(s) 64, 137, 275, 1038
AvaII GGWCC 2 cut(s) 776, 996
BanI GGYRCC 1 cut(s) 34
BbvI GCAGC 4 cut(s) 237, 295, 580, 617
BccI CCATC 1 cut(s) 675
BciT130I CCWGG 1 cut(s) 247
BciVI GTATCC 1 cut(s) 852
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 3 cut(s) 236, 620, 1048
BfaI CTAG 1 cut(s) 924
BfmI CTRYAG 2 cut(s) 309, 334
BfuAI ACCTGC 1 cut(s) 522
BfuI GTATCC 1 cut(s) 852
BisI GCNGC 6 cut(s) 251, 309, 575, 594, 597, 606
BlsI GCNGC 6 cut(s) 252, 310, 576, 595, 598, 607
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 2 cut(s) 776, 996
BmgBI CACGTC 1 cut(s) 655
BmgT120I GGNCC 2 cut(s) 776, 996
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 247
BmsI GCATC 2 cut(s) 45, 817
BpmI CTGGAG 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 403
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 3 cut(s) 526, 575, 820
BsaWI WCCGGW 1 cut(s) 778
BsaXI ACNNNNNCTCC 2 cut(s) 1063, 1093
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 929
Bse118I RCCGGY 2 cut(s) 40, 778
Bse1I ACTGG 3 cut(s) 115, 163, 1107
Bse3DI GCAATG 1 cut(s) 746
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 3 cut(s) 526, 575, 820
BseGI GGATG 2 cut(s) 585, 686
BseLI CCNNNNNNNGG 2 cut(s) 460, 929
BseMI GCAATG 1 cut(s) 746
BseMII CTCAG 2 cut(s) 623, 1099
BseNI ACTGG 3 cut(s) 115, 163, 1107
BseRI GAGGAG 3 cut(s) 223, 341, 837
BseXI GCAGC 4 cut(s) 237, 295, 580, 617
BseYI CCCAGC 1 cut(s) 453
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 577
BshFI GGCC 4 cut(s) 73, 372, 967, 1027
BshNI GGYRCC 1 cut(s) 34
BshTI ACCGGT 1 cut(s) 778
BsiSI CCGG 2 cut(s) 41, 779
BslI CCNNNNNNNGG 2 cut(s) 460, 929
BsmAI GTCTC 3 cut(s) 236, 620, 1048
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 4 cut(s) 73, 372, 967, 1027
Bso31I GGTCTC 1 cut(s) 236
Bsp143I GATC 1 cut(s) 379
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 4 cut(s) 575, 577, 596, 910
BspANI GGCC 4 cut(s) 73, 372, 967, 1027
BspCNI CTCAG 2 cut(s) 624, 1098
BspFNI CGCG 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 36
BspMAI CTGCAG 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 522
BspQI GCTCTTC 1 cut(s) 510
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 746
BsrFI RCCGGY 2 cut(s) 40, 778
BsrI ACTGG 3 cut(s) 115, 163, 1107
BssAI RCCGGY 2 cut(s) 40, 778
BssECI CCNNGG 3 cut(s) 526, 575, 820
BssMI GATC 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 526
Bst2UI CCWGG 1 cut(s) 247
Bst4CI ACNGT 4 cut(s) 81, 335, 397, 932
Bst6I CTCTTC 2 cut(s) 232, 510
BstC8I GCNNGC 3 cut(s) 60, 75, 817
BstDEI CTNAG 3 cut(s) 403, 632, 1085
BstDSI CCRYGG 2 cut(s) 526, 575
BstEII GGTNACC 1 cut(s) 564
BstF5I GGATG 2 cut(s) 585, 686
BstFNI CGCG 1 cut(s) 577
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 3 cut(s) 236, 620, 1048
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 2 cut(s) 602, 605
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 2 cut(s) 296, 767
BstPI GGTNACC 1 cut(s) 564
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 2 cut(s) 309, 334
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 4 cut(s) 237, 295, 580, 617
BstXI CCANNNNNNTGG 1 cut(s) 122
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 4 cut(s) 73, 372, 967, 1027
BtgI CCRYGG 2 cut(s) 526, 575
BtrI CACGTC 1 cut(s) 655
BtsCI GGATG 2 cut(s) 585, 686
BtsIMutI CAGTG 3 cut(s) 77, 97, 629
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 3 cut(s) 60, 75, 817
Cfr10I RCCGGY 2 cut(s) 40, 778
Cfr13I GGNCC 2 cut(s) 776, 996
Cfr42I CCGCGG 1 cut(s) 578
CseI GACGC 1 cut(s) 506
Csp6I GTAC 2 cut(s) 761, 933
CspAI ACCGGT 1 cut(s) 778
CviAII CATG 6 cut(s) 293, 527, 677, 715, 764, 913
CviQI GTAC 2 cut(s) 761, 933
DdeI CTNAG 3 cut(s) 403, 632, 1085
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 232, 510
EarI CTCTTC 2 cut(s) 232, 510
Eco130I CCWWGG 1 cut(s) 526
Eco147I AGGCCT 2 cut(s) 967, 1027
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 776, 996
Eco57I CTGAAG 2 cut(s) 534, 999
Eco91I GGTNACC 1 cut(s) 564
EcoO65I GGTNACC 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 526
EcoT22I ATGCAT 1 cut(s) 832
ErhI CCWWGG 1 cut(s) 526
FaeI CATG 6 cut(s) 296, 530, 680, 718, 767, 916
FalI AAGNNNNNCTT 4 cut(s) 281, 313, 928, 960
FatI CATG 6 cut(s) 292, 526, 676, 714, 763, 912
FauNDI CATATG 4 cut(s) 193, 496, 832, 1006
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 6 cut(s) 251, 309, 575, 594, 597, 606
FokI GGATG 2 cut(s) 592, 693
Fsp4HI GCNGC 6 cut(s) 251, 309, 575, 594, 597, 606
FspBI CTAG 1 cut(s) 924
GluI GCNGC 6 cut(s) 251, 309, 575, 594, 597, 606
GsaI CCCAGC 1 cut(s) 457
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 4 cut(s) 73, 372, 967, 1027
HapII CCGG 2 cut(s) 41, 779
HgaI GACGC 1 cut(s) 506
Hin1II CATG 6 cut(s) 296, 530, 680, 718, 767, 916
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HindIII AAGCTT 2 cut(s) 218, 400
HinfI GANTC 2 cut(s) 342, 916
HpaII CCGG 2 cut(s) 41, 779
HphI GGTGA 4 cut(s) 64, 137, 275, 1038
Hpy166II GTNNAC 3 cut(s) 391, 616, 776
Hpy188I TCNGA 3 cut(s) 24, 897, 979
Hpy188III TCNNGA 2 cut(s) 298, 702
Hpy8I GTNNAC 3 cut(s) 391, 616, 776
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 5 cut(s) 739, 974, 1004, 1017, 1031
HpyCH4III ACNGT 4 cut(s) 81, 335, 397, 932
HpyCH4IV ACGT 2 cut(s) 413, 654
HpyF10VI GCNNNNNNNGC 2 cut(s) 602, 605
HpyF3I CTNAG 3 cut(s) 403, 632, 1085
HpySE526I ACGT 2 cut(s) 413, 654
Hsp92II CATG 6 cut(s) 296, 530, 680, 718, 767, 916
Ksp22I TGATCA 1 cut(s) 379
KspI CCGCGG 1 cut(s) 578
Kzo9I GATC 1 cut(s) 379
LguI GCTCTTC 1 cut(s) 510
LmnI GCTCC 2 cut(s) 161, 181
Lsp1109I GCAGC 4 cut(s) 237, 295, 580, 617
LweI GCATC 2 cut(s) 45, 817
MaeI CTAG 1 cut(s) 924
MaeII ACGT 2 cut(s) 413, 654
MaeIII GTNAC 3 cut(s) 430, 564, 926
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 6 cut(s) 140, 159, 249, 497, 761, 911
MfeI CAATTG 1 cut(s) 666
MluCI AATT 8 cut(s) 25, 153, 186, 303, 469, 666, 801, 898
MmeI TCCRAC 3 cut(s) 441, 1014, 1094
Mph1103I ATGCAT 1 cut(s) 832
MseI TTAA 4 cut(s) 12, 348, 543, 800
MslI CAYNNNNRTG 2 cut(s) 443, 768
MspA1I CMGCKG 2 cut(s) 457, 577
MspI CCGG 2 cut(s) 41, 779
MspR9I CCNGG 1 cut(s) 247
MunI CAATTG 1 cut(s) 666
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 1 cut(s) 247
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 2 cut(s) 602, 605
NcoI CCATGG 1 cut(s) 526
NdeI CATATG 4 cut(s) 193, 496, 832, 1006
NdeII GATC 1 cut(s) 379
NlaIII CATG 6 cut(s) 296, 530, 680, 718, 767, 916
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 2 cut(s) 398, 845
NmuCI GTSAC 1 cut(s) 926
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 2 cut(s) 296, 767
OliI CACNNNNGTG 1 cut(s) 443
PceI AGGCCT 2 cut(s) 967, 1027
PciSI GCTCTTC 1 cut(s) 510
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 342, 916
PflMI CCANNNNNTGG 1 cut(s) 460
PinAI ACCGGT 1 cut(s) 778
PkrI GCNGC 6 cut(s) 252, 310, 576, 595, 598, 607
PshBI ATTAAT 1 cut(s) 348
PsiI TTATAA 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 453
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 2 cut(s) 776, 996
PstI CTGCAG 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 457
RsaI GTAC 2 cut(s) 762, 934
RsaNI GTAC 2 cut(s) 761, 933
RseI CAYNNNNRTG 2 cut(s) 443, 768
SacII CCGCGG 1 cut(s) 578
SapI GCTCTTC 1 cut(s) 510
SaqAI TTAA 4 cut(s) 12, 348, 543, 800
SatI GCNGC 6 cut(s) 251, 309, 575, 594, 597, 606
Sau3AI GATC 1 cut(s) 379
Sau96I GGNCC 2 cut(s) 776, 996
ScrFI CCNGG 1 cut(s) 247
SfaNI GCATC 2 cut(s) 45, 817
SfcI CTRYAG 2 cut(s) 309, 334
Sfr303I CCGCGG 1 cut(s) 578
SgrBI CCGCGG 1 cut(s) 578
SinI GGWCC 2 cut(s) 776, 996
SmiMI CAYNNNNRTG 2 cut(s) 443, 768
Sse9I AATT 8 cut(s) 25, 153, 186, 303, 469, 666, 801, 898
SseBI AGGCCT 2 cut(s) 967, 1027
SsiI CCGC 4 cut(s) 575, 577, 596, 910
SspI AATATT 1 cut(s) 892
SspMI CTAG 1 cut(s) 924
StuI AGGCCT 2 cut(s) 967, 1027
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 4 cut(s) 81, 335, 397, 932
TaiI ACGT 2 cut(s) 416, 657
TaqI TCGA 1 cut(s) 701
TasI AATT 8 cut(s) 25, 153, 186, 303, 469, 666, 801, 898
TauI GCSGC 2 cut(s) 577, 599
TfiI GAWTC 2 cut(s) 342, 916
Tru1I TTAA 4 cut(s) 12, 348, 543, 800
Tru9I TTAA 4 cut(s) 12, 348, 543, 800
TscAI CASTG 3 cut(s) 84, 97, 636
TseFI GTSAC 1 cut(s) 926
TseI GCWGC 4 cut(s) 250, 308, 593, 605
Tsp45I GTSAC 1 cut(s) 926
TspDTI ATGAA 7 cut(s) 76, 210, 334, 366, 703, 993, 1023
TspRI CASTG 3 cut(s) 84, 97, 636
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 2 cut(s) 776, 996
VspI ATTAAT 1 cut(s) 348
XapI RAATTY 2 cut(s) 25, 469
XceI RCATGY 2 cut(s) 296, 767
XspI CTAG 1 cut(s) 924
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.