MD15G1397600.v1.1

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013147
Physical Location & Seq
Forward (+)
49801490 .. 49804779
3290 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1397600.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGATTTGTTTAACTCGGTGTTGAATTGGGTGGTGCCACCGGCAAGTTTAGTGATGCTGGCGTTTGCTTGGCCAGCGTTGACCTTCGTCACTGGTTGTGAGTGGCTTTACAATTTGTTCAACAGTGAAATTGTCGAAGATAAGGTTGTTATAATTACAGGAGCTTCTTCTGGCATAGGAGAGCAAATTGCATATGAATATGCGAAGAGAAGAGCAAATCTTGTACTAGTGGCACGAAGACAGAACAGACTGCGAGTGATCAGTGAGAATGCAAGGCGTATGGGTGCAAAGCATGTCATGAATATAGCCGCCGACGTTGTCAAGGAAGAGGAATGTAGGCGTTTCATCAGTGAAACGATAAGCTTCTATGGCCGAGTGGATCACCTTGTAAATACAGTAAGTCTAGGACATACATTCTACTTCGAGGAAGTTACAGACACGTCTGTCTTTCCCCAATTGTTGGATATAAATTTCTGGGGGAATGTTTATCCAACATATGTTGCTCTTCCTTACCTACGTCAAACTAATGGAAGGATCATTGTCAATGCATCAGTTGAGAGCTGGTTACCTCTGCCGAGAATGAGTTTATATGCTGCGGCAAAGGCAGCTCTGGTTAACTTTTATGAGACCTTGAGATTAGAAGTAAACCATGAGGTAGGGATAACAATTGCAACTCACGGGTGGATTGGAAGTGAAATGACAGGAGGCAAGTTCATGCTAGAGGAGGGGGCTGAAATGCAGTGGAAAGAAGAAAGGGAGGTACATGTGACCGGTGGGTCTGTGGAGGATTTTGCAAGGTTGATTGTAGCTGGGGCTTGTCGAGGAGATTCATACGTTAAGTATCCAAGTTGGTATGACATATTCCTGCTGTACAGGGTGTTTGCGCCTAAGGTCCTGAATTGGACATTCCGCCTCATTCTTCCAACGCAAGGTGGAAGGAGGACTTCCCTTGTGGGCACCGGAAGGCCTATTTCTGAAGGCTATGGGAGGCCTATTTCTGAAGGCTATGGGAGGCCTATTTCAGAAGGCTATGGAAGGCCTTTGTTGGAAGGCACTCCTCCAAGGAGACTTATTGGTCCCAACACAGTTTCCCAACAGAGTCCCCCGCGTCAGATAAAAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

376

Amino Acids

42.31

Weight (kDa)

7.72

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 48 - 235 1.3e-36 short chain dehydrogenase
adh_short_C2 PF13561 54 - 233 1.1e-20 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AasI GACNNNNNNGTC 3 cut(s) 443, 775, 1074
AccB1I GGYRCC 2 cut(s) 34, 956
AccB7I CCANNNNNTGG 1 cut(s) 460
AccII CGCG 1 cut(s) 1108
AciI CCGC 4 cut(s) 309, 596, 910, 1106
AclWI GGATC 2 cut(s) 387, 542
AcoI YGGCCR 2 cut(s) 71, 370
AcsI RAATTY 1 cut(s) 469
AcuI CTGAAG 2 cut(s) 996, 1020
AfaI GTAC 3 cut(s) 225, 762, 872
AfiI CCNNNNNNNGG 2 cut(s) 460, 929
AflIII ACRYGT 2 cut(s) 438, 763
AgeI ACCGGT 1 cut(s) 770
AgsI TTSAA 2 cut(s) 25, 121
AhlI ACTAGT 1 cut(s) 226
AjiI CACGTC 1 cut(s) 441
AluBI AGCT 5 cut(s) 164, 363, 561, 608, 809
AluI AGCT 5 cut(s) 164, 363, 561, 608, 809
Alw26I GTCTC 2 cut(s) 620, 1060
AlwI GGATC 2 cut(s) 387, 542
AoxI GGCC 6 cut(s) 71, 370, 965, 989, 1013, 1037
ApeKI GCWGC 2 cut(s) 593, 605
ApoI RAATTY 1 cut(s) 469
AsiGI ACCGGT 1 cut(s) 770
AspLEI GCGC 1 cut(s) 886
AspS9I GGNCC 2 cut(s) 892, 1076
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 2 cut(s) 892, 1076
AxyI CCTNAGG 1 cut(s) 888
BaeGI GKGCMC 1 cut(s) 959
BalI TGGCCA 1 cut(s) 73
BanI GGYRCC 2 cut(s) 34, 956
BbsI GAAGAC 1 cut(s) 244
BbvI GCAGC 2 cut(s) 580, 617
BciVI GTATCC 1 cut(s) 852
BclI TGATCA 1 cut(s) 258
BcoDI GTCTC 2 cut(s) 620, 1060
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 3 cut(s) 227, 404, 719
BfuI GTATCC 1 cut(s) 852
BisI GCNGC 4 cut(s) 309, 594, 597, 606
BlsI GCNGC 4 cut(s) 310, 595, 598, 607
Bme18I GGWCC 2 cut(s) 892, 1076
BmgBI CACGTC 1 cut(s) 441
BmgT120I GGNCC 2 cut(s) 892, 1076
BmiI GGNNCC 3 cut(s) 36, 958, 1078
BmsI GCATC 2 cut(s) 45, 557
BoxI GACNNNNGTC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 244
BpuEI CTTGAG 1 cut(s) 652
BsaI GGTCTC 1 cut(s) 620
BsaJI CCNNGG 1 cut(s) 1061
BsaWI WCCGGW 2 cut(s) 770, 959
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 929
Bse118I RCCGGY 2 cut(s) 40, 770
Bse1I ACTGG 1 cut(s) 97
Bse21I CCTNAGG 1 cut(s) 888
BseDI CCNNGG 1 cut(s) 1061
BseLI CCNNNNNNNGG 2 cut(s) 460, 929
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 3 cut(s) 737, 837, 1047
BseSI GKGCMC 1 cut(s) 959
BseXI GCAGC 2 cut(s) 580, 617
BseYI CCCAGC 1 cut(s) 809
Bsh1236I CGCG 1 cut(s) 1108
BshFI GGCC 6 cut(s) 73, 372, 967, 991, 1015, 1039
BshNI GGYRCC 2 cut(s) 34, 956
BshTI ACCGGT 1 cut(s) 770
BsiSI CCGG 3 cut(s) 41, 771, 960
BslFI GGGAC 2 cut(s) 1062, 1086
BslI CCNNNNNNNGG 2 cut(s) 460, 929
BsmAI GTCTC 2 cut(s) 620, 1060
BsmFI GGGAC 2 cut(s) 1062, 1086
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 6 cut(s) 73, 372, 967, 991, 1015, 1039
Bso31I GGTCTC 1 cut(s) 620
Bsp1286I GDGCHC 1 cut(s) 959
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 3 cut(s) 258, 379, 534
BspACI CCGC 4 cut(s) 309, 596, 910, 1106
BspANI GGCC 6 cut(s) 73, 372, 967, 991, 1015, 1039
BspFNI CGCG 1 cut(s) 1108
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 36, 958, 1078
BspPI GGATC 2 cut(s) 387, 542
BspQI GCTCTTC 2 cut(s) 205, 510
BspT107I GGYRCC 2 cut(s) 34, 956
BspTNI GGTCTC 1 cut(s) 620
BsrFI RCCGGY 2 cut(s) 40, 770
BsrGI TGTACA 1 cut(s) 870
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 2 cut(s) 40, 770
BssECI CCNNGG 1 cut(s) 1061
BssMI GATC 3 cut(s) 258, 379, 534
BssT1I CCWWGG 1 cut(s) 1061
Bst4CI ACNGT 3 cut(s) 125, 397, 1087
Bst6I CTCTTC 4 cut(s) 200, 205, 321, 510
BstAUI TGTACA 1 cut(s) 870
BstC8I GCNNGC 2 cut(s) 60, 75
BstDEI CTNAG 1 cut(s) 888
BstEII GGTNACC 1 cut(s) 564
BstFNI CGCG 1 cut(s) 1108
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 3 cut(s) 261, 382, 537
BstMAI GTCTC 2 cut(s) 620, 1060
BstMBI GATC 3 cut(s) 258, 379, 534
BstMWI GCNNNNNNNGC 4 cut(s) 74, 369, 602, 605
BstNSI RCATGY 2 cut(s) 296, 767
BstPAI GACNNNNGTC 1 cut(s) 86
BstPI GGTNACC 1 cut(s) 564
BstSLI GKGCMC 1 cut(s) 959
BstUI CGCG 1 cut(s) 1108
BstV1I GCAGC 2 cut(s) 580, 617
BstV2I GAAGAC 1 cut(s) 244
Bsu36I CCTNAGG 1 cut(s) 888
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 6 cut(s) 73, 372, 967, 991, 1015, 1039
BtrI CACGTC 1 cut(s) 441
BtsI GCAGTG 1 cut(s) 746
BtsIMutI CAGTG 5 cut(s) 90, 130, 268, 355, 746
Cac8I GCNNGC 2 cut(s) 60, 75
CciI TCATGA 1 cut(s) 297
CfoI GCGC 1 cut(s) 886
Cfr10I RCCGGY 2 cut(s) 40, 770
Cfr13I GGNCC 2 cut(s) 892, 1076
CseI GACGC 1 cut(s) 1097
Csp6I GTAC 3 cut(s) 224, 761, 871
CspAI ACCGGT 1 cut(s) 770
CviAII CATG 5 cut(s) 293, 298, 650, 715, 764
CviQI GTAC 3 cut(s) 224, 761, 871
DdeI CTNAG 1 cut(s) 888
DpnI GATC 3 cut(s) 260, 381, 536
DpnII GATC 3 cut(s) 258, 379, 534
DrdI GACNNNNNNGTC 3 cut(s) 443, 775, 1074
DseDI GACNNNNNNGTC 3 cut(s) 443, 775, 1074
EaeI YGGCCR 2 cut(s) 71, 370
Eam1104I CTCTTC 4 cut(s) 200, 205, 321, 510
EarI CTCTTC 4 cut(s) 200, 205, 321, 510
EciI GGCGGA 1 cut(s) 899
Eco130I CCWWGG 1 cut(s) 1061
Eco147I AGGCCT 4 cut(s) 967, 991, 1015, 1039
Eco31I GGTCTC 1 cut(s) 620
Eco47I GGWCC 2 cut(s) 892, 1076
Eco57I CTGAAG 2 cut(s) 996, 1020
Eco81I CCTNAGG 1 cut(s) 888
Eco91I GGTNACC 1 cut(s) 564
EcoO109I RGGNCCY 1 cut(s) 892
EcoO65I GGTNACC 1 cut(s) 564
EcoT14I CCWWGG 1 cut(s) 1061
EcoT22I ATGCAT 1 cut(s) 550
ErhI CCWWGG 1 cut(s) 1061
FaeI CATG 5 cut(s) 296, 301, 653, 718, 767
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 2 cut(s) 1062, 1086
FatI CATG 5 cut(s) 292, 297, 649, 714, 763
FauI CCCGC 1 cut(s) 1113
FauNDI CATATG 2 cut(s) 193, 496
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 4 cut(s) 309, 594, 597, 606
Fsp4HI GCNGC 4 cut(s) 309, 594, 597, 606
FspBI CTAG 3 cut(s) 227, 404, 719
GlaI GCGC 1 cut(s) 885
GluI GCNGC 4 cut(s) 309, 594, 597, 606
GsaI CCCAGC 1 cut(s) 813
HaeIII GGCC 6 cut(s) 73, 372, 967, 991, 1015, 1039
HapII CCGG 3 cut(s) 41, 771, 960
HgaI GACGC 1 cut(s) 1097
HhaI GCGC 1 cut(s) 886
Hin1II CATG 5 cut(s) 296, 301, 653, 718, 767
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HincII GTYRAC 2 cut(s) 81, 616
HindII GTYRAC 2 cut(s) 81, 616
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 2 cut(s) 827, 1099
HpaI GTTAAC 1 cut(s) 616
HpaII CCGG 3 cut(s) 41, 771, 960
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 3 cut(s) 81, 616, 646
Hpy188I TCNGA 4 cut(s) 976, 1000, 1024, 1113
Hpy188III TCNNGA 2 cut(s) 298, 895
Hpy8I GTNNAC 3 cut(s) 81, 616, 646
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 9 cut(s) 94, 525, 930, 957, 971, 995, 1019, 1029, 1043
HpyCH4III ACNGT 3 cut(s) 125, 397, 1087
HpyCH4IV ACGT 4 cut(s) 315, 440, 517, 834
HpyCH4V TGCA 7 cut(s) 191, 272, 287, 548, 671, 739, 794
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 369, 602, 605
HpyF3I CTNAG 1 cut(s) 888
HpySE526I ACGT 4 cut(s) 315, 440, 517, 834
Hsp92II CATG 5 cut(s) 296, 301, 653, 718, 767
HspAI GCGC 1 cut(s) 884
Ksp22I TGATCA 1 cut(s) 258
KspAI GTTAAC 1 cut(s) 616
Kzo9I GATC 3 cut(s) 258, 379, 534
LguI GCTCTTC 2 cut(s) 205, 510
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 2 cut(s) 580, 617
LweI GCATC 2 cut(s) 45, 557
MaeI CTAG 3 cut(s) 227, 404, 719
MaeII ACGT 4 cut(s) 315, 440, 517, 834
MaeIII GTNAC 4 cut(s) 88, 430, 564, 766
MalI GATC 3 cut(s) 260, 381, 536
MboI GATC 3 cut(s) 258, 379, 534
MboII GAAGA 9 cut(s) 149, 159, 217, 222, 249, 338, 497, 761, 911
MfeI CAATTG 2 cut(s) 455, 666
MhlI GDGCHC 1 cut(s) 959
MlsI TGGCCA 1 cut(s) 73
MluCI AATT 9 cut(s) 25, 112, 129, 153, 186, 455, 469, 666, 898
MluNI TGGCCA 1 cut(s) 73
MlyI GAGTC 1 cut(s) 1108
MmeI TCCRAC 4 cut(s) 441, 515, 947, 1026
Mox20I TGGCCA 1 cut(s) 73
Mph1103I ATGCAT 1 cut(s) 550
MscI TGGCCA 1 cut(s) 73
MseI TTAA 3 cut(s) 12, 615, 837
Msp20I TGGCCA 1 cut(s) 73
MspI CCGG 3 cut(s) 41, 771, 960
MunI CAATTG 2 cut(s) 455, 666
Mva1269I GAATGC 1 cut(s) 274
MvnI CGCG 1 cut(s) 1108
MwoI GCNNNNNNNGC 4 cut(s) 74, 369, 602, 605
NdeI CATATG 2 cut(s) 193, 496
NdeII GATC 3 cut(s) 258, 379, 534
NlaIII CATG 5 cut(s) 296, 301, 653, 718, 767
NlaIV GGNNCC 3 cut(s) 36, 958, 1078
NmeAIII GCCGAG 2 cut(s) 398, 600
NmuCI GTSAC 2 cut(s) 88, 766
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 2 cut(s) 296, 767
PagI TCATGA 1 cut(s) 297
PceI AGGCCT 4 cut(s) 967, 991, 1015, 1039
PciI ACATGT 1 cut(s) 763
PciSI GCTCTTC 2 cut(s) 205, 510
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 827
PflFI GACNNNGTC 1 cut(s) 317
PflMI CCANNNNNTGG 1 cut(s) 460
PinAI ACCGGT 1 cut(s) 770
PkrI GCNGC 4 cut(s) 310, 595, 598, 607
PleI GAGTC 1 cut(s) 1107
PpsI GAGTC 1 cut(s) 1107
PpuMI RGGWCCY 1 cut(s) 892
PscI ACATGT 1 cut(s) 763
PshAI GACNNNNGTC 1 cut(s) 86
PsiI TTATAA 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 892
PspEI GGTNACC 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 809
PspN4I GGNNCC 3 cut(s) 36, 958, 1078
PspPI GGNCC 2 cut(s) 892, 1076
PspPPI RGGWCCY 1 cut(s) 892
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 3 cut(s) 225, 762, 872
RsaNI GTAC 3 cut(s) 224, 761, 871
SapI GCTCTTC 2 cut(s) 205, 510
SaqAI TTAA 3 cut(s) 12, 615, 837
SatI GCNGC 4 cut(s) 309, 594, 597, 606
Sau3AI GATC 3 cut(s) 258, 379, 534
Sau96I GGNCC 2 cut(s) 892, 1076
SchI GAGTC 1 cut(s) 1108
SduI GDGCHC 1 cut(s) 959
SfaNI GCATC 2 cut(s) 45, 557
SinI GGWCC 2 cut(s) 892, 1076
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
SpeI ACTAGT 1 cut(s) 226
Sse9I AATT 9 cut(s) 25, 112, 129, 153, 186, 455, 469, 666, 898
SseBI AGGCCT 4 cut(s) 967, 991, 1015, 1039
SsiI CCGC 4 cut(s) 309, 596, 910, 1106
SspMI CTAG 3 cut(s) 227, 404, 719
StuI AGGCCT 4 cut(s) 967, 991, 1015, 1039
StyI CCWWGG 1 cut(s) 1061
TaaI ACNGT 3 cut(s) 125, 397, 1087
TaiI ACGT 4 cut(s) 318, 443, 520, 837
TaqI TCGA 3 cut(s) 135, 423, 820
TasI AATT 9 cut(s) 25, 112, 129, 153, 186, 455, 469, 666, 898
TatI WGTACW 2 cut(s) 223, 870
TauI GCSGC 2 cut(s) 311, 599
TfiI GAWTC 1 cut(s) 827
Tru1I TTAA 3 cut(s) 12, 615, 837
Tru9I TTAA 3 cut(s) 12, 615, 837
TscAI CASTG 5 cut(s) 97, 130, 268, 355, 746
TseFI GTSAC 2 cut(s) 88, 766
TseI GCWGC 2 cut(s) 593, 605
Tsp45I GTSAC 2 cut(s) 88, 766
TspDTI ATGAA 5 cut(s) 210, 314, 334, 703, 819
TspRI CASTG 5 cut(s) 97, 130, 268, 355, 746
Tth111I GACNNNGTC 1 cut(s) 317
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 2 cut(s) 892, 1076
XapI RAATTY 1 cut(s) 469
XceI RCATGY 2 cut(s) 296, 767
XspI CTAG 3 cut(s) 227, 404, 719
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.