FvH4_5g33170

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013147
Physical Location & Seq
Forward (+)
23960119 .. 23963808
3690 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33170.t1

Sequence Viewer

Length: 1083 bp
ATGGATTTGTTTAACTCGGTTCTGAATTTCGTGGTGCCACCGGCAAGTTTGGTGATGCTGGCTTTTTCTTGGCCTGCATTGTGCTTCATCAGTGGTCTCGAGTGGCTTTACAACCAGTTCAATGGTGAAATTATGGAGGACAAAGTTGTTATAATCACTGGAGCTTCTTCTGGCATAGGGGAGCAAATTGCATATGAATATGCAAAGAGGAGAGCGCAGCTTGTATTAGTGGCAAGAAGAGACACCAGACTGCGAGGGATTGCTGAGAATGCAAGGTTCTTGGGTGCAAAGCATGTTTTGATAATTGCTGCTGATGTTGTCAAGGAGGAGGACTGTAGGAGATTCATTAATGAAACCATAAACATTTACGGCCGAGTGGATCATCTGGTAAACACGGTAAGCTTGGGACACACGTTCAGCTTTGAGGAAGTTACAGACACCTCTGTGTTCCCCCAGCTGTTGGACATAAATTTTTGGGGAAATGTTTATCCTACATATGTTGCTCTTCCTTACCTGCGTCAAACCCATGGAAAAGTCATTGTGAATGCTTCAGTTGAGAGCTGGTTACCTTTGCCGCGGATGAGCTTATATGCTGCGGCAAAGGCAGCTCTAGTCAACTTTTATGAGACGCTGAGATTAGAAGTAAGAGACGAGGTGGGGATAACAATTGCAACTCATGGATGGATTGTTAGTGAAATGTCAAGAGGCAAGTTCATGGTAGATGAGGGTGCTGAAATGCAATGGAAGGAGGAAAGAGAGGTACAAGCGAGCGGTGGACCGGTGGAGGATTTTGCAAGGTTAATTGTAGCAGGGGCTTGTCGAGGAGATCCATATGTGAAGTATCCAAGCTGGTATGACATATTCCTGCTTTACAGGGTGTTTGCACCGAATATTCTGAATTGGACATTCCGCATGATTCTTCCAACTCATGGTACAAGAAGAACTTCACTCGTGGGCACGGGGAGGCCTATTTCATCTGAAGGTAGAAATAATGGAAGGCTTTTGTTGGAAGGAACTTCACCAAGGAGACATCTTGGTAGTCCAACTTTCACTCCACTCGGTCACCAGCACAAACTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

361

Amino Acids

40.64

Weight (kDa)

6.77

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 48 - 235 3.9e-36 short chain dehydrogenase
adh_short_C2 PF13561 54 - 236 1e-20 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 34
AccB7I CCANNNNNTGG 2 cut(s) 460, 929
AccBSI CCGCTC 1 cut(s) 771
AccII CGCG 1 cut(s) 577
AciI CCGC 5 cut(s) 575, 577, 596, 771, 910
AclWI GGATC 2 cut(s) 387, 821
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 2 cut(s) 25, 469
AcuI CTGAAG 2 cut(s) 534, 999
AfaI GTAC 2 cut(s) 762, 934
AfiI CCNNNNNNNGG 2 cut(s) 460, 929
AflIII ACRYGT 1 cut(s) 411
AgeI ACCGGT 1 cut(s) 778
AgsI TTSAA 1 cut(s) 121
AleI CACNNNNGTG 1 cut(s) 443
AluBI AGCT 9 cut(s) 164, 220, 402, 420, 457, 561, 585, 608, 849
AluI AGCT 9 cut(s) 164, 220, 402, 420, 457, 561, 585, 608, 849
Alw26I GTCTC 5 cut(s) 101, 234, 620, 642, 1021
AlwI GGATC 2 cut(s) 387, 821
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 3 cut(s) 71, 370, 965
ApeKI GCWGC 4 cut(s) 217, 308, 593, 605
ApoI RAATTY 2 cut(s) 25, 469
AseI ATTAAT 1 cut(s) 348
AsiGI ACCGGT 1 cut(s) 778
Asp700I GAANNNNTTC 1 cut(s) 943
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 776
AsuHPI GGTGA 4 cut(s) 64, 137, 1011, 1055
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 776
BaeGI GKGCMC 1 cut(s) 959
BanI GGYRCC 1 cut(s) 34
BauI CACGAG 1 cut(s) 950
BbvI GCAGC 4 cut(s) 229, 295, 580, 617
BccI CCATC 1 cut(s) 675
BceAI ACGGC 1 cut(s) 385
BciVI GTATCC 1 cut(s) 852
BcoDI GTCTC 5 cut(s) 101, 234, 620, 642, 1021
BfaI CTAG 1 cut(s) 611
BfmI CTRYAG 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 522
BfuI GTATCC 1 cut(s) 852
BisI GCNGC 6 cut(s) 218, 309, 575, 594, 597, 606
BlsI GCNGC 6 cut(s) 219, 310, 576, 595, 598, 607
Bme18I GGWCC 1 cut(s) 776
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 776
BmiI GGNNCC 1 cut(s) 36
BmsI GCATC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 3 cut(s) 526, 575, 1022
BsaWI WCCGGW 1 cut(s) 778
BsaXI ACNNNNNCTCC 2 cut(s) 1036, 1066
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 929
Bse118I RCCGGY 2 cut(s) 40, 778
Bse1I ACTGG 3 cut(s) 115, 163, 1080
Bse3DI GCAATG 1 cut(s) 746
BseDI CCNNGG 3 cut(s) 526, 575, 1022
BseGI GGATG 2 cut(s) 585, 686
BseLI CCNNNNNNNGG 2 cut(s) 460, 929
BseMI GCAATG 1 cut(s) 746
BseMII CTCAG 2 cut(s) 255, 623
BseNI ACTGG 3 cut(s) 115, 163, 1080
BseRI GAGGAG 3 cut(s) 223, 341, 837
BseSI GKGCMC 1 cut(s) 959
BseX3I CGGCCG 1 cut(s) 370
BseXI GCAGC 4 cut(s) 229, 295, 580, 617
BseYI CCCAGC 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 577
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 3 cut(s) 73, 372, 967
BshNI GGYRCC 1 cut(s) 34
BshTI ACCGGT 1 cut(s) 778
BsiEI CGRYCG 1 cut(s) 373
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 2 cut(s) 41, 779
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 2 cut(s) 460, 929
BsmAI GTCTC 5 cut(s) 101, 234, 620, 642, 1021
BsmBI CGTCTC 2 cut(s) 620, 642
BsmFI GGGAC 1 cut(s) 420
BsmI GAATGC 2 cut(s) 274, 550
BsnI GGCC 3 cut(s) 73, 372, 967
Bso31I GGTCTC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 959
Bsp143I GATC 2 cut(s) 379, 826
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 5 cut(s) 575, 577, 596, 771, 910
BspANI GGCC 3 cut(s) 73, 372, 967
BspCNI CTCAG 2 cut(s) 256, 624
BspFNI CGCG 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 36
BspMI ACCTGC 1 cut(s) 522
BspPI GGATC 2 cut(s) 387, 821
BspQI GCTCTTC 1 cut(s) 510
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 771
BsrDI GCAATG 1 cut(s) 746
BsrFI RCCGGY 2 cut(s) 40, 778
BsrI ACTGG 3 cut(s) 115, 163, 1080
BssAI RCCGGY 2 cut(s) 40, 778
BssECI CCNNGG 3 cut(s) 526, 575, 1022
BssMI GATC 2 cut(s) 379, 826
BssSI CACGAG 1 cut(s) 950
BssT1I CCWWGG 2 cut(s) 526, 1022
Bst2BI CACGAG 1 cut(s) 950
Bst4CI ACNGT 2 cut(s) 335, 397
Bst6I CTCTTC 2 cut(s) 232, 510
BstC8I GCNNGC 3 cut(s) 60, 75, 769
BstDEI CTNAG 2 cut(s) 264, 632
BstDSI CCRYGG 2 cut(s) 526, 575
BstEII GGTNACC 2 cut(s) 564, 1061
BstF5I GGATG 2 cut(s) 585, 686
BstFNI CGCG 1 cut(s) 577
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 2 cut(s) 382, 829
BstMAI GTCTC 5 cut(s) 101, 234, 620, 642, 1021
BstMBI GATC 2 cut(s) 379, 826
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 3 cut(s) 269, 602, 605
BstNSI RCATGY 1 cut(s) 296
BstPI GGTNACC 2 cut(s) 564, 1061
BstSFI CTRYAG 1 cut(s) 334
BstSLI GKGCMC 1 cut(s) 959
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 4 cut(s) 229, 295, 580, 617
BstX2I RGATCY 1 cut(s) 826
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 826
BstZI CGGCCG 1 cut(s) 370
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 3 cut(s) 73, 372, 967
BtgI CCRYGG 2 cut(s) 526, 575
BtsCI GGATG 2 cut(s) 585, 686
BtsIMutI CAGTG 2 cut(s) 97, 156
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 3 cut(s) 60, 75, 769
CfoI GCGC 1 cut(s) 217
Cfr10I RCCGGY 2 cut(s) 40, 778
Cfr13I GGNCC 1 cut(s) 776
Cfr42I CCGCGG 1 cut(s) 578
CseI GACGC 2 cut(s) 506, 637
Csp6I GTAC 2 cut(s) 761, 933
CspAI ACCGGT 1 cut(s) 778
CviAII CATG 6 cut(s) 293, 527, 677, 715, 913, 929
CviQI GTAC 2 cut(s) 761, 933
DdeI CTNAG 2 cut(s) 264, 632
DpnI GATC 2 cut(s) 381, 828
DpnII GATC 2 cut(s) 379, 826
EaeI YGGCCR 1 cut(s) 370
EagI CGGCCG 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 232, 510
EarI CTCTTC 2 cut(s) 232, 510
EclXI CGGCCG 1 cut(s) 370
Eco130I CCWWGG 2 cut(s) 526, 1022
Eco147I AGGCCT 1 cut(s) 967
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 776
Eco52I CGGCCG 1 cut(s) 370
Eco57I CTGAAG 2 cut(s) 534, 999
Eco88I CYCGRG 1 cut(s) 98
Eco91I GGTNACC 2 cut(s) 564, 1061
EcoO65I GGTNACC 2 cut(s) 564, 1061
EcoT14I CCWWGG 2 cut(s) 526, 1022
ErhI CCWWGG 2 cut(s) 526, 1022
Esp3I CGTCTC 2 cut(s) 620, 642
FaeI CATG 6 cut(s) 296, 530, 680, 718, 916, 932
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 1 cut(s) 420
FatI CATG 6 cut(s) 292, 526, 676, 714, 912, 928
FauNDI CATATG 3 cut(s) 193, 496, 832
Fnu4HI GCNGC 6 cut(s) 218, 309, 575, 594, 597, 606
FokI GGATG 2 cut(s) 592, 693
Fsp4HI GCNGC 6 cut(s) 218, 309, 575, 594, 597, 606
FspBI CTAG 1 cut(s) 611
GlaI GCGC 1 cut(s) 216
GluI GCNGC 6 cut(s) 218, 309, 575, 594, 597, 606
GsaI CCCAGC 1 cut(s) 457
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 3 cut(s) 73, 372, 967
HapII CCGG 2 cut(s) 41, 779
HgaI GACGC 2 cut(s) 506, 637
HhaI GCGC 1 cut(s) 217
Hin1II CATG 6 cut(s) 296, 530, 680, 718, 916, 932
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 2 cut(s) 342, 916
HpaII CCGG 2 cut(s) 41, 779
HphI GGTGA 4 cut(s) 64, 137, 1011, 1055
Hpy166II GTNNAC 3 cut(s) 391, 616, 776
Hpy188I TCNGA 3 cut(s) 24, 897, 979
Hpy188III TCNNGA 2 cut(s) 98, 702
Hpy8I GTNNAC 3 cut(s) 391, 616, 776
HpyAV CCTTC 4 cut(s) 739, 974, 990, 1004
HpyCH4III ACNGT 2 cut(s) 335, 397
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 9 cut(s) 77, 191, 203, 272, 287, 671, 739, 794, 884
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 602, 605
HpyF3I CTNAG 2 cut(s) 264, 632
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 6 cut(s) 296, 530, 680, 718, 916, 932
HspAI GCGC 1 cut(s) 215
KspI CCGCGG 1 cut(s) 578
Kzo9I GATC 2 cut(s) 379, 826
LguI GCTCTTC 1 cut(s) 510
LmnI GCTCC 2 cut(s) 161, 181
Lsp1109I GCAGC 4 cut(s) 229, 295, 580, 617
LweI GCATC 1 cut(s) 45
MaeI CTAG 1 cut(s) 611
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 3 cut(s) 430, 564, 1061
MalI GATC 2 cut(s) 381, 828
MbiI CCGCTC 1 cut(s) 771
MboI GATC 2 cut(s) 379, 826
MboII GAAGA 5 cut(s) 159, 249, 497, 911, 951
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 1 cut(s) 959
MluCI AATT 8 cut(s) 25, 129, 186, 303, 469, 666, 801, 898
MmeI TCCRAC 4 cut(s) 441, 947, 987, 1067
MroXI GAANNNNTTC 1 cut(s) 943
MseI TTAA 3 cut(s) 12, 348, 800
MslI CAYNNNNRTG 1 cut(s) 443
MspA1I CMGCKG 2 cut(s) 457, 577
MspI CCGG 2 cut(s) 41, 779
MunI CAATTG 1 cut(s) 666
Mva1269I GAATGC 2 cut(s) 274, 550
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 3 cut(s) 269, 602, 605
NcoI CCATGG 1 cut(s) 526
NdeI CATATG 3 cut(s) 193, 496, 832
NdeII GATC 2 cut(s) 379, 826
NlaIII CATG 6 cut(s) 296, 530, 680, 718, 916, 932
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 1 cut(s) 1061
NspI RCATGY 1 cut(s) 296
OliI CACNNNNGTG 1 cut(s) 443
PaeR7I CTCGAG 1 cut(s) 98
PceI AGGCCT 1 cut(s) 967
PciSI GCTCTTC 1 cut(s) 510
PctI GAATGC 2 cut(s) 274, 550
PdmI GAANNNNTTC 1 cut(s) 943
PfeI GAWTC 2 cut(s) 342, 916
PflMI CCANNNNNTGG 2 cut(s) 460, 929
PinAI ACCGGT 1 cut(s) 778
PkrI GCNGC 6 cut(s) 219, 310, 576, 595, 598, 607
PshBI ATTAAT 1 cut(s) 348
PsiI TTATAA 1 cut(s) 152
PspEI GGTNACC 2 cut(s) 564, 1061
PspFI CCCAGC 1 cut(s) 453
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 776
PsuI RGATCY 1 cut(s) 826
PvuII CAGCTG 1 cut(s) 457
RsaI GTAC 2 cut(s) 762, 934
RsaNI GTAC 2 cut(s) 761, 933
RseI CAYNNNNRTG 1 cut(s) 443
SacII CCGCGG 1 cut(s) 578
SapI GCTCTTC 1 cut(s) 510
SaqAI TTAA 3 cut(s) 12, 348, 800
SatI GCNGC 6 cut(s) 218, 309, 575, 594, 597, 606
Sau3AI GATC 2 cut(s) 379, 826
Sau96I GGNCC 1 cut(s) 776
SduI GDGCHC 1 cut(s) 959
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 98
Sfr303I CCGCGG 1 cut(s) 578
SgrBI CCGCGG 1 cut(s) 578
SinI GGWCC 1 cut(s) 776
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 443
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 8 cut(s) 25, 129, 186, 303, 469, 666, 801, 898
SseBI AGGCCT 1 cut(s) 967
SsiI CCGC 5 cut(s) 575, 577, 596, 771, 910
SspI AATATT 1 cut(s) 892
SspMI CTAG 1 cut(s) 611
StuI AGGCCT 1 cut(s) 967
StyI CCWWGG 2 cut(s) 526, 1022
TaaI ACNGT 2 cut(s) 335, 397
TaiI ACGT 1 cut(s) 416
TaqI TCGA 2 cut(s) 99, 820
TaqII GACCGA 1 cut(s) 1049
TasI AATT 8 cut(s) 25, 129, 186, 303, 469, 666, 801, 898
TauI GCSGC 2 cut(s) 577, 599
TfiI GAWTC 2 cut(s) 342, 916
Tru1I TTAA 3 cut(s) 12, 348, 800
Tru9I TTAA 3 cut(s) 12, 348, 800
TscAI CASTG 2 cut(s) 97, 163
TseFI GTSAC 1 cut(s) 1061
TseI GCWGC 4 cut(s) 217, 308, 593, 605
Tsp45I GTSAC 1 cut(s) 1061
TspDTI ATGAA 6 cut(s) 76, 210, 334, 366, 703, 963
TspRI CASTG 2 cut(s) 97, 163
Van91I CCANNNNNTGG 2 cut(s) 460, 929
VpaK11BI GGWCC 1 cut(s) 776
VspI ATTAAT 1 cut(s) 348
XapI RAATTY 2 cut(s) 25, 469
XceI RCATGY 1 cut(s) 296
XhoI CTCGAG 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 943
XspI CTAG 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.