MD08G1214700.v1.1

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013147
Physical Location & Seq
Forward (+)
27744591 .. 27747875
3285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1214700.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGATTTGTTTAACTCGGTGCTGAATTGGGTGGTGCCACCGGCAAGTTTAGTGATGCTGGCGTTTGCTTGGCCAGCGTTGACCTTCATCAGTGGTTGTGAGTGGCTTTACAATTTGTTCAACAGTGAAATTGTCGAAGATAAGGTGGTTATAATTACAGGAGCTTCTTCTGGCATAGGAGAGCAAATTGCATACGAATATGCAAAGAGGAGGGCGAATCTTGTACTAGTGGCACGAAGAGAGAACAGACTGCGTGTGATCAGTGAGAATGCAAGGCTTATAGGTGCAAAGCATGTCATGATTATAGCCGCAGATGTTGTAAAGGAAGAGGAATGCAGGAGATTTATCAGTGAAACGATAAGCTTCCATGGCCGAGTGGATCACCTTGTAAATACAGTAAGTCTAGGACACACATTCTACTTCGAGGAAGTTACAGACACGTCTGTCTTTCCCCTTTTGTTGGATATAAATTTCTGGGGGAATGTATATCCAACATATGTTGCTCTTCCTTACCTACGTCGAACCAATGGAAAGATAATTGTTAATGCATCAGTTGAGAGCTGGTTACCTCTACCGAGAATGAGTTTATATGCTGCAGCAAAGGCAGCTCTGGTAAACTTTTATGAGACCTTGAGATTAGAAGTAAATCATGAGGTAGGGATAACAATTGCAACTCACGGATGGATTGGAAGTGAGATGACAAGAGGCAAGTTCATGCTAGAGGAGGGCGCCGAAATGCAATGGAAAGAAGAAAGGGAGGTACTGGTACATGTAATCGGTGGGCCTGTGGAAGATTTTGCCAGGTTGATTGTAGCTGGGGCTTGTCAAGGAGATTCGTACGTAAAGTATCCAAGCTGGTATGACATATTCCTGCTGTACAGGGTGTTTGTGCCTAATATCCTGCATTGGACATTCCGCCTCATTCTTCCAACGCACGGTGGAAGGAGGACTTCCCTTGTGGGCACCGGGGGGCCTATTTCTGAAGGCTATGGAAGGCCTTTGTTGGAAGGCACTCCTCCAAGGAGATTTGTTGGTCCCAACACAATTTCCCAACAGAGTCCCTCACGTCCGCTAAAAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

362

Amino Acids

40.75

Weight (kDa)

6.53

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 48 - 236 2.6e-35 short chain dehydrogenase
adh_short_C2 PF13561 54 - 217 1.6e-19 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AasI GACNNNNNNGTC 1 cut(s) 443
AccB1I GGYRCC 3 cut(s) 34, 728, 962
AciI CCGC 3 cut(s) 309, 916, 1070
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 2 cut(s) 71, 370
AcsI RAATTY 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 1002
AcyI GRCGYC 1 cut(s) 729
AfaI GTAC 5 cut(s) 225, 762, 768, 839, 878
AfiI CCNNNNNNNGG 2 cut(s) 460, 935
AflIII ACRYGT 2 cut(s) 438, 769
AgsI TTSAA 1 cut(s) 121
AhlI ACTAGT 1 cut(s) 226
AjiI CACGTC 2 cut(s) 441, 1067
AjnI CCWGG 1 cut(s) 800
AluBI AGCT 6 cut(s) 164, 363, 561, 608, 815, 855
AluI AGCT 6 cut(s) 164, 363, 561, 608, 815, 855
Alw26I GTCTC 1 cut(s) 620
AlwI GGATC 1 cut(s) 387
AoxI GGCC 5 cut(s) 71, 370, 782, 971, 995
ApeKI GCWGC 3 cut(s) 593, 596, 605
ApoI RAATTY 1 cut(s) 469
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 3 cut(s) 782, 971, 1034
AsuC2I CCSGG 1 cut(s) 967
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 1 cut(s) 1034
BaeGI GKGCMC 1 cut(s) 965
BalI TGGCCA 1 cut(s) 73
BanI GGYRCC 3 cut(s) 34, 728, 962
BbvI GCAGC 3 cut(s) 580, 608, 617
BccI CCATC 1 cut(s) 675
BciT130I CCWGG 1 cut(s) 802
BciVI GTATCC 1 cut(s) 858
BclI TGATCA 1 cut(s) 258
BcnI CCSGG 1 cut(s) 967
BcoDI GTCTC 1 cut(s) 620
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 3 cut(s) 227, 404, 719
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 1 cut(s) 732
BfuI GTATCC 1 cut(s) 858
BisI GCNGC 4 cut(s) 309, 594, 597, 606
BlsI GCNGC 4 cut(s) 310, 595, 598, 607
Bme1390I CCNGG 2 cut(s) 802, 967
Bme18I GGWCC 1 cut(s) 1034
BmgBI CACGTC 2 cut(s) 441, 1067
BmgT120I GGNCC 3 cut(s) 782, 971, 1034
BmiI GGNNCC 5 cut(s) 36, 730, 964, 972, 1036
BmrFI CCNGG 2 cut(s) 802, 967
BmsI GCATC 2 cut(s) 45, 557
BpuEI CTTGAG 1 cut(s) 652
BpuMI CCSGG 1 cut(s) 967
BsaAI YACGTR 1 cut(s) 841
BsaHI GRCGYC 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 620
BsaJI CCNNGG 3 cut(s) 367, 966, 1019
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 935
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 768
Bse3DI GCAATG 1 cut(s) 746
BseBI CCWGG 1 cut(s) 802
BseDI CCNNGG 3 cut(s) 367, 966, 1019
BseGI GGATG 1 cut(s) 686
BseLI CCNNNNNNNGG 2 cut(s) 460, 935
BseMI GCAATG 1 cut(s) 746
BseNI ACTGG 1 cut(s) 768
BseRI GAGGAG 3 cut(s) 223, 737, 1005
BseSI GKGCMC 1 cut(s) 965
BseXI GCAGC 3 cut(s) 580, 608, 617
BseYI CCCAGC 1 cut(s) 815
BshFI GGCC 5 cut(s) 73, 372, 784, 973, 997
BshNI GGYRCC 3 cut(s) 34, 728, 962
BsiSI CCGG 2 cut(s) 41, 966
BsiWI CGTACG 1 cut(s) 837
BslFI GGGAC 2 cut(s) 1020, 1044
BslI CCNNNNNNNGG 2 cut(s) 460, 935
BsmAI GTCTC 1 cut(s) 620
BsmFI GGGAC 2 cut(s) 1020, 1044
BsmI GAATGC 2 cut(s) 274, 338
BsnI GGCC 5 cut(s) 73, 372, 784, 973, 997
Bso31I GGTCTC 1 cut(s) 620
Bsp1286I GDGCHC 1 cut(s) 965
Bsp1407I TGTACA 1 cut(s) 876
Bsp143I GATC 2 cut(s) 258, 379
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 3 cut(s) 309, 916, 1070
BspANI GGCC 5 cut(s) 73, 372, 784, 973, 997
BspHI TCATGA 2 cut(s) 297, 649
BspLI GGNNCC 5 cut(s) 36, 730, 964, 972, 1036
BspMAI CTGCAG 1 cut(s) 598
BspPI GGATC 1 cut(s) 387
BspQI GCTCTTC 1 cut(s) 510
BspT107I GGYRCC 3 cut(s) 34, 728, 962
BspTNI GGTCTC 1 cut(s) 620
BsrDI GCAATG 1 cut(s) 746
BsrFI RCCGGY 1 cut(s) 40
BsrGI TGTACA 1 cut(s) 876
BsrI ACTGG 1 cut(s) 768
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 3 cut(s) 367, 966, 1019
BssMI GATC 2 cut(s) 258, 379
BssNI GRCGYC 1 cut(s) 729
BssT1I CCWWGG 2 cut(s) 367, 1019
Bst2UI CCWGG 1 cut(s) 802
Bst4CI ACNGT 3 cut(s) 125, 397, 938
Bst6I CTCTTC 3 cut(s) 232, 321, 510
BstACI GRCGYC 1 cut(s) 729
BstAUI TGTACA 1 cut(s) 876
BstBAI YACGTR 1 cut(s) 841
BstC8I GCNNGC 2 cut(s) 60, 75
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 564
BstF5I GGATG 1 cut(s) 686
BstH2I RGCGCY 1 cut(s) 732
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 2 cut(s) 261, 382
BstMAI GTCTC 1 cut(s) 620
BstMBI GATC 2 cut(s) 258, 379
BstMWI GCNNNNNNNGC 4 cut(s) 74, 369, 602, 605
BstNI CCWGG 1 cut(s) 802
BstNSI RCATGY 2 cut(s) 296, 773
BstPI GGTNACC 1 cut(s) 564
BstSCI CCNGG 2 cut(s) 800, 965
BstSFI CTRYAG 1 cut(s) 594
BstSLI GKGCMC 1 cut(s) 965
BstSNI TACGTA 1 cut(s) 841
BstV1I GCAGC 3 cut(s) 580, 608, 617
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 5 cut(s) 73, 372, 784, 973, 997
BtgI CCRYGG 1 cut(s) 367
BtrI CACGTC 2 cut(s) 441, 1067
BtsCI GGATG 1 cut(s) 686
BtsIMutI CAGTG 4 cut(s) 97, 130, 268, 355
Cac8I GCNNGC 2 cut(s) 60, 75
CciI TCATGA 2 cut(s) 297, 649
CfoI GCGC 1 cut(s) 731
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 782, 971, 1034
Csp6I GTAC 5 cut(s) 224, 761, 767, 838, 877
CviAII CATG 6 cut(s) 293, 298, 368, 650, 715, 770
CviQI GTAC 5 cut(s) 224, 761, 767, 838, 877
DinI GGCGCC 1 cut(s) 730
DpnI GATC 2 cut(s) 260, 381
DpnII GATC 2 cut(s) 258, 379
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
EaeI YGGCCR 2 cut(s) 71, 370
Eam1104I CTCTTC 3 cut(s) 232, 321, 510
EarI CTCTTC 3 cut(s) 232, 321, 510
EciI GGCGGA 1 cut(s) 905
Eco105I TACGTA 1 cut(s) 841
Eco130I CCWWGG 2 cut(s) 367, 1019
Eco147I AGGCCT 1 cut(s) 997
Eco31I GGTCTC 1 cut(s) 620
Eco47I GGWCC 1 cut(s) 1034
Eco57I CTGAAG 1 cut(s) 1002
Eco91I GGTNACC 1 cut(s) 564
EcoO109I RGGNCCY 1 cut(s) 971
EcoO65I GGTNACC 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 800
EcoT14I CCWWGG 2 cut(s) 367, 1019
EcoT22I ATGCAT 1 cut(s) 550
EgeI GGCGCC 1 cut(s) 730
EheI GGCGCC 1 cut(s) 730
ErhI CCWWGG 2 cut(s) 367, 1019
FaeI CATG 6 cut(s) 296, 301, 371, 653, 718, 773
FalI AAGNNNNNCTT 2 cut(s) 934, 966
FaqI GGGAC 2 cut(s) 1020, 1044
FatI CATG 6 cut(s) 292, 297, 367, 649, 714, 769
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 4 cut(s) 309, 594, 597, 606
FokI GGATG 1 cut(s) 693
Fsp4HI GCNGC 4 cut(s) 309, 594, 597, 606
FspBI CTAG 3 cut(s) 227, 404, 719
GlaI GCGC 1 cut(s) 730
GluI GCNGC 4 cut(s) 309, 594, 597, 606
GsaI CCCAGC 1 cut(s) 819
HaeII RGCGCY 1 cut(s) 732
HaeIII GGCC 5 cut(s) 73, 372, 784, 973, 997
HapII CCGG 2 cut(s) 41, 966
HhaI GCGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 729
Hin1II CATG 6 cut(s) 296, 301, 371, 653, 718, 773
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 3 cut(s) 217, 833, 1057
HpaII CCGG 2 cut(s) 41, 966
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 81, 616
Hpy188I TCNGA 1 cut(s) 982
Hpy188III TCNNGA 2 cut(s) 298, 650
Hpy8I GTNNAC 2 cut(s) 81, 616
Hpy99I CGWCG 1 cut(s) 522
HpyAV CCTTC 5 cut(s) 94, 936, 977, 987, 1001
HpyCH4III ACNGT 3 cut(s) 125, 397, 938
HpyCH4IV ACGT 4 cut(s) 440, 517, 840, 1066
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 369, 602, 605
HpySE526I ACGT 4 cut(s) 440, 517, 840, 1066
Hsp92I GRCGYC 1 cut(s) 729
Hsp92II CATG 6 cut(s) 296, 301, 371, 653, 718, 773
HspAI GCGC 1 cut(s) 729
KasI GGCGCC 1 cut(s) 728
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 2 cut(s) 258, 379
LguI GCTCTTC 1 cut(s) 510
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 3 cut(s) 580, 608, 617
LweI GCATC 2 cut(s) 45, 557
MaeI CTAG 3 cut(s) 227, 404, 719
MaeII ACGT 4 cut(s) 440, 517, 840, 1066
MaeIII GTNAC 2 cut(s) 430, 564
MalI GATC 2 cut(s) 260, 381
MboI GATC 2 cut(s) 258, 379
MboII GAAGA 8 cut(s) 149, 159, 249, 338, 497, 761, 803, 917
MfeI CAATTG 1 cut(s) 666
MhlI GDGCHC 1 cut(s) 965
MlsI TGGCCA 1 cut(s) 73
MluCI AATT 9 cut(s) 25, 112, 129, 153, 186, 469, 537, 666, 1044
MluNI TGGCCA 1 cut(s) 73
Mly113I GGCGCC 1 cut(s) 729
MlyI GAGTC 1 cut(s) 1066
MmeI TCCRAC 4 cut(s) 441, 515, 953, 984
Mox20I TGGCCA 1 cut(s) 73
Mph1103I ATGCAT 1 cut(s) 550
MscI TGGCCA 1 cut(s) 73
MseI TTAA 2 cut(s) 12, 543
Msp20I TGGCCA 1 cut(s) 73
MspI CCGG 2 cut(s) 41, 966
MspR9I CCNGG 2 cut(s) 802, 967
MunI CAATTG 1 cut(s) 666
Mva1269I GAATGC 2 cut(s) 274, 338
MvaI CCWGG 1 cut(s) 802
MwoI GCNNNNNNNGC 4 cut(s) 74, 369, 602, 605
NarI GGCGCC 1 cut(s) 729
NciI CCSGG 1 cut(s) 967
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 496
NdeII GATC 2 cut(s) 258, 379
NlaIII CATG 6 cut(s) 296, 301, 371, 653, 718, 773
NlaIV GGNNCC 5 cut(s) 36, 730, 964, 972, 1036
NmeAIII GCCGAG 1 cut(s) 398
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 2 cut(s) 296, 773
PagI TCATGA 2 cut(s) 297, 649
PceI AGGCCT 1 cut(s) 997
PciI ACATGT 1 cut(s) 769
PciSI GCTCTTC 1 cut(s) 510
PctI GAATGC 2 cut(s) 274, 338
PfeI GAWTC 2 cut(s) 217, 833
Pfl23II CGTACG 1 cut(s) 837
PkrI GCNGC 4 cut(s) 310, 595, 598, 607
PleI GAGTC 1 cut(s) 1065
PluTI GGCGCC 1 cut(s) 732
PpsI GAGTC 1 cut(s) 1065
Ppu21I YACGTR 1 cut(s) 841
PscI ACATGT 1 cut(s) 769
PsiI TTATAA 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 800
PspEI GGTNACC 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 1 cut(s) 800
PspLI CGTACG 1 cut(s) 837
PspN4I GGNNCC 5 cut(s) 36, 730, 964, 972, 1036
PspPI GGNCC 3 cut(s) 782, 971, 1034
PstI CTGCAG 1 cut(s) 598
RsaI GTAC 5 cut(s) 225, 762, 768, 839, 878
RsaNI GTAC 5 cut(s) 224, 761, 767, 838, 877
SapI GCTCTTC 1 cut(s) 510
SaqAI TTAA 2 cut(s) 12, 543
SatI GCNGC 4 cut(s) 309, 594, 597, 606
Sau3AI GATC 2 cut(s) 258, 379
Sau96I GGNCC 3 cut(s) 782, 971, 1034
SchI GAGTC 1 cut(s) 1066
ScrFI CCNGG 2 cut(s) 802, 967
SduI GDGCHC 1 cut(s) 965
SfaNI GCATC 2 cut(s) 45, 557
SfcI CTRYAG 1 cut(s) 594
SfoI GGCGCC 1 cut(s) 730
SinI GGWCC 1 cut(s) 1034
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
SnaBI TACGTA 1 cut(s) 841
SpeI ACTAGT 1 cut(s) 226
Sse9I AATT 9 cut(s) 25, 112, 129, 153, 186, 469, 537, 666, 1044
SseBI AGGCCT 1 cut(s) 997
SsiI CCGC 3 cut(s) 309, 916, 1070
SspDI GGCGCC 1 cut(s) 728
SspMI CTAG 3 cut(s) 227, 404, 719
StuI AGGCCT 1 cut(s) 997
StyD4I CCNGG 2 cut(s) 800, 965
StyI CCWWGG 2 cut(s) 367, 1019
TaaI ACNGT 3 cut(s) 125, 397, 938
TaiI ACGT 4 cut(s) 443, 520, 843, 1069
TaqI TCGA 3 cut(s) 135, 423, 520
TasI AATT 9 cut(s) 25, 112, 129, 153, 186, 469, 537, 666, 1044
TatI WGTACW 2 cut(s) 223, 876
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 2 cut(s) 217, 833
Tru1I TTAA 2 cut(s) 12, 543
Tru9I TTAA 2 cut(s) 12, 543
TscAI CASTG 4 cut(s) 97, 130, 268, 355
TseI GCWGC 3 cut(s) 593, 596, 605
TspDTI ATGAA 2 cut(s) 76, 703
TspGWI ACGGA 1 cut(s) 693
TspRI CASTG 4 cut(s) 97, 130, 268, 355
VpaK11BI GGWCC 1 cut(s) 1034
XapI RAATTY 1 cut(s) 469
XceI RCATGY 2 cut(s) 296, 773
XspI CTAG 3 cut(s) 227, 404, 719
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.