RchiOBHm_Chr7g0231461

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013147
Physical Location & Seq
Forward (+)
55079145 .. 55087270
8126 bp
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UTR
Exon/CDS
Intron
PRQ20743

Sequence Viewer

Length: 1191 bp
ATGAGCTTCCAAACCAACACGTGTTTCTCACAAACACTCATGCATGTAAGGATACTGCTGTTGATCTCACCATTTGCCACTACATGTCAAACTCGAATCGATCTTATCTCCAGCTTCCGTCCCAGTCTCTCATTTCGATCATCAATATCAGTTACCAGTTTGGTGATGCTGGCTTTTTCTTGGCCTGCACTGTGCTTCATCAGTGGTTGCGAGTGGCTTTACAACCAGTTCAATGGTGAAGATATGGAGGACAAAGTGGTTATAATTACTGGAGCTTCTTCTGGCATAGGGGAGCAAATTGCATATGAATATGCAAAGAGGCGGGCGAAGCTTGTATTAGTGGCAAGAAGAGAGACCAGGCTGCGAGGGATTGGTGAGAATGCAAGGTTCTTGGGTGCAAAGCATGTCTTGATAATTGCTGCAGATGTTGTCAAGGAGGAGGACTGTAGGAGGTTCATTAATGAAACCATAAACATTTACGGCCGAGTGGATCATCTTGTAAACACAGTAAGCTTAGGACACACGTTCAGCTTTGAGGAAGTTACAGACACTTCTGTGTTTCCCCAGCTGTTGGACATAAATTTTTGGGGAAATGTTTATCCTACATATGTTGCTCTTCCTTACCTGCGTCAAACCCATGGAAAAGTCATTGTTAATGCTTCAGTTGAGAGCTGGTTACCTTTGCCGCGGATGAGTTTATATGCTGCGGCAAAGGCAGCTCTGGTCAACTTTTATGAGACACTGAGATTAGAAGTAAAAGACGACGTGGGGATAACAATTGCAACTCATGGATGGATTGTTAGTGAAATGTCAAGAGGCAAGTTCATGGTAGATGAGGGTGCTGAAATGCAATGGAAGGAAGAAAGAGAGGTACATGTGAGTGGTGGACCGGCGGAGGATTTTGCAAGGTTAATTGTAGCAGGGGCTTGCCGAGGAGATGCATACGTGAAGTATCCAAGCTGGTATGACATATTCCTGCTTTACAGGGTGTTTGCACCTAATATTCTGAATTGGACATTCCGCATGATTCTTCCTACACACGGTATGAGGAGAACTTCACTCATTGGCACGGGGAGGCCTATTTCATCTGAAGGTAGCTATGGAAGGCCTTTGTTGGAAGGAACTTCACCAAGGAGACTTCTTGTTAGTCCAACTTTCACTCCACTCAGTCATCAGCACAAACTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

396

Amino Acids

44.74

Weight (kDa)

8.16

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 85 - 272 7.9e-36 short chain dehydrogenase
adh_short_C2 PF13561 91 - 272 5.2e-20 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 633
AccB7I CCANNNNNTGG 1 cut(s) 571
AccII CGCG 1 cut(s) 688
AciI CCGC 6 cut(s) 322, 686, 688, 707, 893, 1021
AclWI GGATC 1 cut(s) 498
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 1 cut(s) 580
AcuI CTGAAG 2 cut(s) 645, 1110
AcvI CACGTG 1 cut(s) 21
AfaI GTAC 1 cut(s) 873
AfiI CCNNNNNNNGG 2 cut(s) 571, 1040
AflIII ACRYGT 5 cut(s) 18, 20, 83, 522, 874
AgsI TTSAA 1 cut(s) 232
AjiI CACGTC 1 cut(s) 766
AjnI CCWGG 1 cut(s) 356
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AleI CACNNNNGTG 1 cut(s) 554
Alw26I GTCTC 4 cut(s) 131, 347, 731, 1129
AlwI GGATC 1 cut(s) 498
AoxI GGCC 4 cut(s) 182, 481, 1076, 1106
ApeKI GCWGC 4 cut(s) 361, 419, 704, 716
ApoI RAATTY 1 cut(s) 580
AseI ATTAAT 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 887
AsuHPI GGTGA 5 cut(s) 60, 175, 248, 386, 1119
AvaII GGWCC 1 cut(s) 887
BaeI ACNNNNGTAYC 2 cut(s) 44, 77
BbrPI CACGTG 1 cut(s) 21
BbvI GCAGC 4 cut(s) 348, 406, 691, 728
BccI CCATC 1 cut(s) 786
BceAI ACGGC 1 cut(s) 496
BciT130I CCWGG 1 cut(s) 358
BciVI GTATCC 2 cut(s) 45, 963
BcoDI GTCTC 4 cut(s) 131, 347, 731, 1129
BfmI CTRYAG 2 cut(s) 420, 445
BfuAI ACCTGC 1 cut(s) 633
BfuI GTATCC 2 cut(s) 45, 963
BisI GCNGC 6 cut(s) 362, 420, 686, 705, 708, 717
BlsI GCNGC 6 cut(s) 363, 421, 687, 706, 709, 718
Bme1390I CCNGG 1 cut(s) 358
Bme18I GGWCC 1 cut(s) 887
BmgBI CACGTC 1 cut(s) 766
BmgT120I GGNCC 1 cut(s) 887
BmrFI CCNGG 1 cut(s) 358
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 156, 928
BmuI ACTGGG 1 cut(s) 117
BpmI CTGGAG 2 cut(s) 94, 291
Bpu10I CCTNAGC 1 cut(s) 514
Bsa29I ATCGAT 1 cut(s) 99
BsaAI YACGTR 2 cut(s) 21, 946
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 4 cut(s) 637, 686, 931, 1130
BsaXI ACNNNNNCTCC 2 cut(s) 1144, 1174
Bsc4I CCNNNNNNNGG 2 cut(s) 571, 1040
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 5 cut(s) 123, 156, 226, 274, 1188
Bse3DI GCAATG 1 cut(s) 857
BseBI CCWGG 1 cut(s) 358
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 4 cut(s) 637, 686, 931, 1130
BseGI GGATG 2 cut(s) 696, 797
BseLI CCNNNNNNNGG 2 cut(s) 571, 1040
BseMI GCAATG 1 cut(s) 857
BseMII CTCAG 2 cut(s) 734, 1180
BseNI ACTGG 5 cut(s) 123, 156, 226, 274, 1188
BseRI GAGGAG 3 cut(s) 452, 948, 1063
BseX3I CGGCCG 1 cut(s) 481
BseXI GCAGC 4 cut(s) 348, 406, 691, 728
BseYI CCCAGC 1 cut(s) 564
BsgI GTGCAG 1 cut(s) 171
Bsh1236I CGCG 1 cut(s) 688
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 4 cut(s) 184, 483, 1078, 1108
BshVI ATCGAT 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 484
BsiSI CCGG 1 cut(s) 890
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 2 cut(s) 571, 1040
BsmAI GTCTC 4 cut(s) 131, 347, 731, 1129
BsmFI GGGAC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 4 cut(s) 184, 483, 1078, 1108
Bso31I GGTCTC 1 cut(s) 347
Bsp143I GATC 4 cut(s) 63, 100, 137, 490
Bsp19I CCATGG 1 cut(s) 637
BspACI CCGC 6 cut(s) 322, 686, 688, 707, 893, 1021
BspANI GGCC 4 cut(s) 184, 483, 1078, 1108
BspCNI CTCAG 2 cut(s) 735, 1179
BspDI ATCGAT 1 cut(s) 99
BspFNI CGCG 1 cut(s) 688
BspMAI CTGCAG 1 cut(s) 424
BspMI ACCTGC 1 cut(s) 633
BspPI GGATC 1 cut(s) 498
BspQI GCTCTTC 1 cut(s) 621
BspTNI GGTCTC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 857
BsrFI RCCGGY 1 cut(s) 889
BsrI ACTGG 5 cut(s) 123, 156, 226, 274, 1188
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 4 cut(s) 637, 686, 931, 1130
BssMI GATC 4 cut(s) 63, 100, 137, 490
BssT1I CCWWGG 2 cut(s) 637, 1130
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 4 cut(s) 192, 446, 508, 1043
Bst6I CTCTTC 2 cut(s) 343, 621
BstBAI YACGTR 2 cut(s) 21, 946
BstC8I GCNNGC 4 cut(s) 171, 186, 324, 928
BstDEI CTNAG 3 cut(s) 514, 743, 1166
BstDSI CCRYGG 2 cut(s) 637, 686
BstEII GGTNACC 1 cut(s) 675
BstF5I GGATG 2 cut(s) 696, 797
BstFNI CGCG 1 cut(s) 688
BstKTI GATC 4 cut(s) 66, 103, 140, 493
BstMAI GTCTC 4 cut(s) 131, 347, 731, 1129
BstMBI GATC 4 cut(s) 63, 100, 137, 490
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 3 cut(s) 328, 713, 716
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 4 cut(s) 47, 87, 407, 878
BstPI GGTNACC 1 cut(s) 675
BstSCI CCNGG 1 cut(s) 356
BstSFI CTRYAG 2 cut(s) 420, 445
BstUI CGCG 1 cut(s) 688
BstV1I GCAGC 4 cut(s) 348, 406, 691, 728
BstXI CCANNNNNNTGG 1 cut(s) 233
BstZI CGGCCG 1 cut(s) 481
Bsu15I ATCGAT 1 cut(s) 99
BsuI GTATCC 2 cut(s) 45, 963
BsuRI GGCC 4 cut(s) 184, 483, 1078, 1108
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 2 cut(s) 637, 686
BtrI CACGTC 1 cut(s) 766
BtsCI GGATG 2 cut(s) 696, 797
BtsIMutI CAGTG 3 cut(s) 188, 208, 740
BveI ACCTGC 1 cut(s) 633
Cac8I GCNNGC 4 cut(s) 171, 186, 324, 928
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 1 cut(s) 887
Cfr42I CCGCGG 1 cut(s) 689
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 1 cut(s) 617
Csp6I GTAC 1 cut(s) 872
CviAII CATG 9 cut(s) 40, 44, 84, 404, 638, 788, 826, 875, 1024
CviQI GTAC 1 cut(s) 872
DdeI CTNAG 3 cut(s) 514, 743, 1166
DpnI GATC 4 cut(s) 65, 102, 139, 492
DpnII GATC 4 cut(s) 63, 100, 137, 490
EaeI YGGCCR 1 cut(s) 481
EagI CGGCCG 1 cut(s) 481
Eam1104I CTCTTC 2 cut(s) 343, 621
EarI CTCTTC 2 cut(s) 343, 621
EciI GGCGGA 1 cut(s) 908
EclXI CGGCCG 1 cut(s) 481
Eco130I CCWWGG 2 cut(s) 637, 1130
Eco147I AGGCCT 2 cut(s) 1078, 1108
Eco31I GGTCTC 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 887
Eco52I CGGCCG 1 cut(s) 481
Eco57I CTGAAG 2 cut(s) 645, 1110
Eco72I CACGTG 1 cut(s) 21
Eco91I GGTNACC 1 cut(s) 675
EcoO65I GGTNACC 1 cut(s) 675
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 2 cut(s) 637, 1130
EcoT22I ATGCAT 2 cut(s) 45, 943
ErhI CCWWGG 2 cut(s) 637, 1130
FaeI CATG 9 cut(s) 43, 47, 87, 407, 641, 791, 829, 878, 1027
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 1 cut(s) 105
FatI CATG 9 cut(s) 39, 43, 83, 403, 637, 787, 825, 874, 1023
FauI CCCGC 1 cut(s) 315
FauNDI CATATG 2 cut(s) 304, 607
Fnu4HI GCNGC 6 cut(s) 362, 420, 686, 705, 708, 717
FokI GGATG 2 cut(s) 703, 804
Fsp4HI GCNGC 6 cut(s) 362, 420, 686, 705, 708, 717
GluI GCNGC 6 cut(s) 362, 420, 686, 705, 708, 717
GsaI CCCAGC 1 cut(s) 568
GsuI CTGGAG 2 cut(s) 94, 291
HaeIII GGCC 4 cut(s) 184, 483, 1078, 1108
HapII CCGG 1 cut(s) 890
HgaI GACGC 1 cut(s) 617
Hin1II CATG 9 cut(s) 43, 47, 87, 407, 641, 791, 829, 878, 1027
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 2 cut(s) 329, 511
HinfI GANTC 2 cut(s) 96, 1027
HpaII CCGG 1 cut(s) 890
HphI GGTGA 5 cut(s) 60, 175, 248, 386, 1119
Hpy166II GTNNAC 3 cut(s) 502, 727, 887
Hpy188I TCNGA 2 cut(s) 1008, 1090
Hpy188III TCNNGA 2 cut(s) 409, 813
Hpy8I GTNNAC 3 cut(s) 502, 727, 887
Hpy99I CGWCG 1 cut(s) 767
HpyAV CCTTC 4 cut(s) 850, 1085, 1098, 1112
HpyCH4III ACNGT 4 cut(s) 192, 446, 508, 1043
HpyCH4IV ACGT 4 cut(s) 20, 524, 765, 945
HpyF10VI GCNNNNNNNGC 3 cut(s) 328, 713, 716
HpyF3I CTNAG 3 cut(s) 514, 743, 1166
HpySE526I ACGT 4 cut(s) 20, 524, 765, 945
Hsp92II CATG 9 cut(s) 43, 47, 87, 407, 641, 791, 829, 878, 1027
KspI CCGCGG 1 cut(s) 689
Kzo9I GATC 4 cut(s) 63, 100, 137, 490
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 2 cut(s) 272, 292
Lsp1109I GCAGC 4 cut(s) 348, 406, 691, 728
LweI GCATC 2 cut(s) 156, 928
MaeII ACGT 4 cut(s) 20, 524, 765, 945
MaeIII GTNAC 3 cut(s) 151, 541, 675
MalI GATC 4 cut(s) 65, 102, 139, 492
MboI GATC 4 cut(s) 63, 100, 137, 490
MboII GAAGA 6 cut(s) 251, 270, 360, 608, 872, 1022
MfeI CAATTG 1 cut(s) 777
MluCI AATT 7 cut(s) 264, 297, 414, 580, 777, 912, 1009
MmeI TCCRAC 3 cut(s) 552, 1095, 1175
Mph1103I ATGCAT 2 cut(s) 45, 943
MseI TTAA 3 cut(s) 459, 654, 911
MslI CAYNNNNRTG 2 cut(s) 554, 879
MspA1I CMGCKG 2 cut(s) 568, 688
MspI CCGG 1 cut(s) 890
MspR9I CCNGG 1 cut(s) 358
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 1 cut(s) 688
MwoI GCNNNNNNNGC 3 cut(s) 328, 713, 716
NcoI CCATGG 1 cut(s) 637
NdeI CATATG 2 cut(s) 304, 607
NdeII GATC 4 cut(s) 63, 100, 137, 490
NlaIII CATG 9 cut(s) 43, 47, 87, 407, 641, 791, 829, 878, 1027
NmeAIII GCCGAG 2 cut(s) 509, 956
NsiI ATGCAT 2 cut(s) 45, 943
NspI RCATGY 4 cut(s) 47, 87, 407, 878
OliI CACNNNNGTG 1 cut(s) 554
PceI AGGCCT 2 cut(s) 1078, 1108
PciI ACATGT 2 cut(s) 83, 874
PciSI GCTCTTC 1 cut(s) 621
PctI GAATGC 1 cut(s) 385
PfeI GAWTC 2 cut(s) 96, 1027
PflMI CCANNNNNTGG 1 cut(s) 571
PkrI GCNGC 6 cut(s) 363, 421, 687, 706, 709, 718
PmaCI CACGTG 1 cut(s) 21
PmlI CACGTG 1 cut(s) 21
Ppu21I YACGTR 2 cut(s) 21, 946
PscI ACATGT 2 cut(s) 83, 874
PshBI ATTAAT 1 cut(s) 459
PsiI TTATAA 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 356
PspCI CACGTG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 675
PspFI CCCAGC 1 cut(s) 564
PspGI CCWGG 1 cut(s) 356
PspPI GGNCC 1 cut(s) 887
PstI CTGCAG 1 cut(s) 424
PvuII CAGCTG 1 cut(s) 568
RsaI GTAC 1 cut(s) 873
RsaNI GTAC 1 cut(s) 872
RseI CAYNNNNRTG 2 cut(s) 554, 879
SacII CCGCGG 1 cut(s) 689
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 3 cut(s) 459, 654, 911
SatI GCNGC 6 cut(s) 362, 420, 686, 705, 708, 717
Sau3AI GATC 4 cut(s) 63, 100, 137, 490
Sau96I GGNCC 1 cut(s) 887
ScrFI CCNGG 1 cut(s) 358
SfaNI GCATC 2 cut(s) 156, 928
SfcI CTRYAG 2 cut(s) 420, 445
Sfr303I CCGCGG 1 cut(s) 689
SgrBI CCGCGG 1 cut(s) 689
SinI GGWCC 1 cut(s) 887
SmiMI CAYNNNNRTG 2 cut(s) 554, 879
Sse9I AATT 7 cut(s) 264, 297, 414, 580, 777, 912, 1009
SseBI AGGCCT 2 cut(s) 1078, 1108
SsiI CCGC 6 cut(s) 322, 686, 688, 707, 893, 1021
SspI AATATT 1 cut(s) 1003
StuI AGGCCT 2 cut(s) 1078, 1108
StyD4I CCNGG 1 cut(s) 356
StyI CCWWGG 2 cut(s) 637, 1130
TaaI ACNGT 4 cut(s) 192, 446, 508, 1043
TaiI ACGT 4 cut(s) 23, 527, 768, 948
TaqI TCGA 3 cut(s) 94, 99, 136
TasI AATT 7 cut(s) 264, 297, 414, 580, 777, 912, 1009
TauI GCSGC 2 cut(s) 688, 710
TfiI GAWTC 2 cut(s) 96, 1027
Tru1I TTAA 3 cut(s) 459, 654, 911
Tru9I TTAA 3 cut(s) 459, 654, 911
TscAI CASTG 3 cut(s) 195, 208, 747
TseI GCWGC 4 cut(s) 361, 419, 704, 716
TspDTI ATGAA 6 cut(s) 187, 321, 445, 477, 814, 1074
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 3 cut(s) 195, 208, 747
Van91I CCANNNNNTGG 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 887
VspI ATTAAT 1 cut(s) 459
XapI RAATTY 1 cut(s) 580
XceI RCATGY 4 cut(s) 47, 87, 407, 878
Zsp2I ATGCAT 2 cut(s) 45, 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.