Rh7CG441200

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0013147
Physical Location & Seq
Forward (+)
57837216 .. 57840958
3743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG441200.1

Sequence Viewer

Length: 1296 bp
ATGAGCTTCCAAACCAACACGTGTTTCTCACAAACACTCATGCATGTAAGGATACTGCTGTTGATCTCACCATTTGCCACTACATGTCAAACTCGAATCGATCTTATCTCCAGCTTCCGTCCCAGTCTCTCATTTCGATCATCAATATCAGTTACCAGGTTAGCCAAAGGACCATATACTACTACTACCATCCATCTCTTTCAATTTCATAACCTAATGGATTTGCTTAACTCGGTTCTGAATTTCGTGGTGCCACCGGCAAGTTTGGTGATGCTGGCTTTTTCTTGGCCTGCACTGTGCTTCATCAGTGGTTGCGAGTGGCTTTACAACCAGTTCAATGGTGAAGATATGGAGGACAAAGTGGTTATAATTACTGGAGCTTCTTCTGGCATAGGGGAGCAAATTGCATATGAATATGCAAAGAGGCGGGCGAAGCTTGTATTAGTGGCAAGAAGAGAGACCAGGCTGCGAGGGATTGGTGAGAATGCAAGGTTCTTGGGTGCAAAGCATGTCTTGATAATTGCTGCAGATGTTGTCAAGGAGGAGGACTGTAGGAGGTTCATTAATGAAACCATAAACATTTACGGCCGAGTGGATCATCTTGTAAACACAGTAAGCTTAGGACACACGTTCAGCTTTGAGGAAGTTACAGACACTTCTGTGTTTCCCCAGCTGTTGGACATAAATTTTTGGGGAAATGTTTATCCTACATATGTTGCTCTTCCTTACCTGCGTCAAACCCATGGAAAAGTCATTGTTAATGCTTCAGTTGAGAGCTGGTTACCTTTGCCGCGGATGAGTTTATATGCTGCGGCAAAGGCAGCTCTGGTCAACTTTTATGAGACACTGAGATTAGAAGTAAAAGACGACGTGGGGATAACAATTGCAACTCATGGATGGATTGTTAGTGAAATGTCAAGAGGCAAGTTCATGGTAGATGAGGGTGCTGAAATGCAATGGAAGGAAGAAAGAGAGGTACATGTGAGTGGTGGACCGGCGGAGGATTTTGCAAGGTTAATTGTAGCAGGGGCTTGCCGAGGAGATGCATACGTGAAGTATCCAAGCTGGTATGACATATTCCTGCTTTACAGGGTGTTTGCACCTAATATTCTGAATTGGACATTCCGCATGATTCTTCCTACACACGGTATGAGGAGAACTTCACTCATTGGCACGGGGAGGCCTATTTCATCTGAAGGTAGCTATGGAAGGCCTTTGTTGGAAGGAACTTCACCAAGGAGACTTCTTGTTAGTCCAACTTTCACTCCACTCAGTCATCAGCACAAACTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

431

Amino Acids

48.69

Weight (kDa)

8.45

Isoelectric Point (pI)

39.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 120 - 307 9.7e-36 short chain dehydrogenase
adh_short_C2 PF13561 126 - 307 6.4e-20 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 738
AccB1I GGYRCC 1 cut(s) 250
AccB7I CCANNNNNTGG 1 cut(s) 676
AccII CGCG 1 cut(s) 793
AciI CCGC 6 cut(s) 427, 791, 793, 812, 998, 1126
AclWI GGATC 1 cut(s) 603
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 2 cut(s) 241, 685
AcuI CTGAAG 2 cut(s) 750, 1215
AcvI CACGTG 1 cut(s) 21
AfaI GTAC 1 cut(s) 978
AfiI CCNNNNNNNGG 2 cut(s) 676, 1145
AflIII ACRYGT 5 cut(s) 18, 20, 83, 627, 979
AgsI TTSAA 2 cut(s) 203, 337
AjiI CACGTC 1 cut(s) 871
AjnI CCWGG 2 cut(s) 155, 461
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AleI CACNNNNGTG 1 cut(s) 659
Alw26I GTCTC 4 cut(s) 131, 452, 836, 1234
AlwI GGATC 1 cut(s) 603
AoxI GGCC 4 cut(s) 287, 586, 1181, 1211
ApeKI GCWGC 4 cut(s) 466, 524, 809, 821
ApoI RAATTY 2 cut(s) 241, 685
AseI ATTAAT 1 cut(s) 564
AspS9I GGNCC 2 cut(s) 170, 992
AsuHPI GGTGA 5 cut(s) 60, 280, 353, 491, 1224
AvaII GGWCC 2 cut(s) 170, 992
BaeI ACNNNNGTAYC 2 cut(s) 44, 77
BanI GGYRCC 1 cut(s) 250
BbrPI CACGTG 1 cut(s) 21
BbvI GCAGC 4 cut(s) 453, 511, 796, 833
BccI CCATC 3 cut(s) 197, 201, 891
BceAI ACGGC 1 cut(s) 601
BciT130I CCWGG 2 cut(s) 157, 463
BciVI GTATCC 2 cut(s) 45, 1068
BcoDI GTCTC 4 cut(s) 131, 452, 836, 1234
BfmI CTRYAG 2 cut(s) 525, 550
BfuAI ACCTGC 1 cut(s) 738
BfuI GTATCC 2 cut(s) 45, 1068
BisI GCNGC 6 cut(s) 467, 525, 791, 810, 813, 822
BlsI GCNGC 6 cut(s) 468, 526, 792, 811, 814, 823
Bme1390I CCNGG 2 cut(s) 157, 463
Bme18I GGWCC 2 cut(s) 170, 992
BmgBI CACGTC 1 cut(s) 871
BmgT120I GGNCC 2 cut(s) 170, 992
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 2 cut(s) 157, 463
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 261, 1033
BmuI ACTGGG 1 cut(s) 117
BpmI CTGGAG 2 cut(s) 94, 396
Bpu10I CCTNAGC 1 cut(s) 619
Bsa29I ATCGAT 1 cut(s) 99
BsaAI YACGTR 2 cut(s) 21, 1051
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 4 cut(s) 742, 791, 1036, 1235
BsaXI ACNNNNNCTCC 2 cut(s) 1249, 1279
Bsc4I CCNNNNNNNGG 2 cut(s) 676, 1145
Bse118I RCCGGY 2 cut(s) 256, 994
Bse1I ACTGG 4 cut(s) 123, 331, 379, 1293
Bse3DI GCAATG 1 cut(s) 962
BseBI CCWGG 2 cut(s) 157, 463
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 4 cut(s) 742, 791, 1036, 1235
BseGI GGATG 3 cut(s) 189, 801, 902
BseLI CCNNNNNNNGG 2 cut(s) 676, 1145
BseMI GCAATG 1 cut(s) 962
BseMII CTCAG 2 cut(s) 839, 1285
BseNI ACTGG 4 cut(s) 123, 331, 379, 1293
BseRI GAGGAG 3 cut(s) 557, 1053, 1168
BseX3I CGGCCG 1 cut(s) 586
BseXI GCAGC 4 cut(s) 453, 511, 796, 833
BseYI CCCAGC 1 cut(s) 669
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 793
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 4 cut(s) 289, 588, 1183, 1213
BshNI GGYRCC 1 cut(s) 250
BshVI ATCGAT 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 589
BsiSI CCGG 2 cut(s) 257, 995
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 2 cut(s) 676, 1145
BsmAI GTCTC 4 cut(s) 131, 452, 836, 1234
BsmFI GGGAC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 4 cut(s) 289, 588, 1183, 1213
Bso31I GGTCTC 1 cut(s) 452
Bsp143I GATC 4 cut(s) 63, 100, 137, 595
Bsp19I CCATGG 1 cut(s) 742
BspACI CCGC 6 cut(s) 427, 791, 793, 812, 998, 1126
BspANI GGCC 4 cut(s) 289, 588, 1183, 1213
BspCNI CTCAG 2 cut(s) 840, 1284
BspDI ATCGAT 1 cut(s) 99
BspFNI CGCG 1 cut(s) 793
BspLI GGNNCC 1 cut(s) 252
BspMAI CTGCAG 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 738
BspPI GGATC 1 cut(s) 603
BspQI GCTCTTC 1 cut(s) 726
BspT107I GGYRCC 1 cut(s) 250
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 962
BsrFI RCCGGY 2 cut(s) 256, 994
BsrI ACTGG 4 cut(s) 123, 331, 379, 1293
BssAI RCCGGY 2 cut(s) 256, 994
BssECI CCNNGG 4 cut(s) 742, 791, 1036, 1235
BssMI GATC 4 cut(s) 63, 100, 137, 595
BssT1I CCWWGG 2 cut(s) 742, 1235
Bst2UI CCWGG 2 cut(s) 157, 463
Bst4CI ACNGT 4 cut(s) 297, 551, 613, 1148
Bst6I CTCTTC 2 cut(s) 448, 726
BstBAI YACGTR 2 cut(s) 21, 1051
BstC8I GCNNGC 4 cut(s) 276, 291, 429, 1033
BstDEI CTNAG 3 cut(s) 619, 848, 1271
BstDSI CCRYGG 2 cut(s) 742, 791
BstEII GGTNACC 1 cut(s) 780
BstF5I GGATG 3 cut(s) 189, 801, 902
BstFNI CGCG 1 cut(s) 793
BstKTI GATC 4 cut(s) 66, 103, 140, 598
BstMAI GTCTC 4 cut(s) 131, 452, 836, 1234
BstMBI GATC 4 cut(s) 63, 100, 137, 595
BstMCI CGRYCG 1 cut(s) 589
BstMWI GCNNNNNNNGC 3 cut(s) 433, 818, 821
BstNI CCWGG 2 cut(s) 157, 463
BstNSI RCATGY 4 cut(s) 47, 87, 512, 983
BstPI GGTNACC 1 cut(s) 780
BstSCI CCNGG 2 cut(s) 155, 461
BstSFI CTRYAG 2 cut(s) 525, 550
BstUI CGCG 1 cut(s) 793
BstV1I GCAGC 4 cut(s) 453, 511, 796, 833
BstXI CCANNNNNNTGG 1 cut(s) 338
BstZI CGGCCG 1 cut(s) 586
Bsu15I ATCGAT 1 cut(s) 99
BsuI GTATCC 2 cut(s) 45, 1068
BsuRI GGCC 4 cut(s) 289, 588, 1183, 1213
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 2 cut(s) 742, 791
BtrI CACGTC 1 cut(s) 871
BtsCI GGATG 3 cut(s) 189, 801, 902
BtsIMutI CAGTG 3 cut(s) 293, 313, 845
BveI ACCTGC 1 cut(s) 738
Cac8I GCNNGC 4 cut(s) 276, 291, 429, 1033
Cfr10I RCCGGY 2 cut(s) 256, 994
Cfr13I GGNCC 2 cut(s) 170, 992
Cfr42I CCGCGG 1 cut(s) 794
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 1 cut(s) 722
CsiI ACCWGGT 1 cut(s) 155
Csp6I GTAC 1 cut(s) 977
CviAII CATG 9 cut(s) 40, 44, 84, 509, 743, 893, 931, 980, 1129
CviQI GTAC 1 cut(s) 977
DdeI CTNAG 3 cut(s) 619, 848, 1271
DpnI GATC 4 cut(s) 65, 102, 139, 597
DpnII GATC 4 cut(s) 63, 100, 137, 595
EaeI YGGCCR 1 cut(s) 586
EagI CGGCCG 1 cut(s) 586
Eam1104I CTCTTC 2 cut(s) 448, 726
EarI CTCTTC 2 cut(s) 448, 726
EciI GGCGGA 1 cut(s) 1013
EclXI CGGCCG 1 cut(s) 586
Eco130I CCWWGG 2 cut(s) 742, 1235
Eco147I AGGCCT 2 cut(s) 1183, 1213
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 2 cut(s) 170, 992
Eco52I CGGCCG 1 cut(s) 586
Eco57I CTGAAG 2 cut(s) 750, 1215
Eco72I CACGTG 1 cut(s) 21
Eco91I GGTNACC 1 cut(s) 780
EcoO65I GGTNACC 1 cut(s) 780
EcoRII CCWGG 2 cut(s) 155, 461
EcoT14I CCWWGG 2 cut(s) 742, 1235
EcoT22I ATGCAT 2 cut(s) 45, 1048
ErhI CCWWGG 2 cut(s) 742, 1235
FaeI CATG 9 cut(s) 43, 47, 87, 512, 746, 896, 934, 983, 1132
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FaqI GGGAC 1 cut(s) 105
FatI CATG 9 cut(s) 39, 43, 83, 508, 742, 892, 930, 979, 1128
FauI CCCGC 1 cut(s) 420
FauNDI CATATG 2 cut(s) 409, 712
Fnu4HI GCNGC 6 cut(s) 467, 525, 791, 810, 813, 822
FokI GGATG 3 cut(s) 176, 808, 909
Fsp4HI GCNGC 6 cut(s) 467, 525, 791, 810, 813, 822
GluI GCNGC 6 cut(s) 467, 525, 791, 810, 813, 822
GsaI CCCAGC 1 cut(s) 673
GsuI CTGGAG 2 cut(s) 94, 396
HaeIII GGCC 4 cut(s) 289, 588, 1183, 1213
HapII CCGG 2 cut(s) 257, 995
HgaI GACGC 1 cut(s) 722
Hin1II CATG 9 cut(s) 43, 47, 87, 512, 746, 896, 934, 983, 1132
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 2 cut(s) 434, 616
HinfI GANTC 2 cut(s) 96, 1132
HpaII CCGG 2 cut(s) 257, 995
HphI GGTGA 5 cut(s) 60, 280, 353, 491, 1224
Hpy166II GTNNAC 3 cut(s) 607, 832, 992
Hpy188I TCNGA 3 cut(s) 240, 1113, 1195
Hpy188III TCNNGA 2 cut(s) 514, 918
Hpy8I GTNNAC 3 cut(s) 607, 832, 992
Hpy99I CGWCG 1 cut(s) 872
HpyAV CCTTC 4 cut(s) 955, 1190, 1203, 1217
HpyCH4III ACNGT 4 cut(s) 297, 551, 613, 1148
HpyCH4IV ACGT 4 cut(s) 20, 629, 870, 1050
HpyF10VI GCNNNNNNNGC 3 cut(s) 433, 818, 821
HpyF3I CTNAG 3 cut(s) 619, 848, 1271
HpySE526I ACGT 4 cut(s) 20, 629, 870, 1050
Hsp92II CATG 9 cut(s) 43, 47, 87, 512, 746, 896, 934, 983, 1132
KspI CCGCGG 1 cut(s) 794
Kzo9I GATC 4 cut(s) 63, 100, 137, 595
LguI GCTCTTC 1 cut(s) 726
LmnI GCTCC 2 cut(s) 377, 397
Lsp1109I GCAGC 4 cut(s) 453, 511, 796, 833
LweI GCATC 2 cut(s) 261, 1033
MabI ACCWGGT 1 cut(s) 155
MaeII ACGT 4 cut(s) 20, 629, 870, 1050
MaeIII GTNAC 3 cut(s) 151, 646, 780
MalI GATC 4 cut(s) 65, 102, 139, 597
MboI GATC 4 cut(s) 63, 100, 137, 595
MboII GAAGA 6 cut(s) 356, 375, 465, 713, 977, 1127
MfeI CAATTG 1 cut(s) 882
MluCI AATT 9 cut(s) 203, 241, 369, 402, 519, 685, 882, 1017, 1114
MmeI TCCRAC 3 cut(s) 657, 1200, 1280
Mph1103I ATGCAT 2 cut(s) 45, 1048
MseI TTAA 4 cut(s) 228, 564, 759, 1016
MslI CAYNNNNRTG 2 cut(s) 659, 984
MspA1I CMGCKG 2 cut(s) 673, 793
MspI CCGG 2 cut(s) 257, 995
MspR9I CCNGG 2 cut(s) 157, 463
MunI CAATTG 1 cut(s) 882
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 2 cut(s) 157, 463
MvnI CGCG 1 cut(s) 793
MwoI GCNNNNNNNGC 3 cut(s) 433, 818, 821
NcoI CCATGG 1 cut(s) 742
NdeI CATATG 2 cut(s) 409, 712
NdeII GATC 4 cut(s) 63, 100, 137, 595
NlaIII CATG 9 cut(s) 43, 47, 87, 512, 746, 896, 934, 983, 1132
NlaIV GGNNCC 1 cut(s) 252
NmeAIII GCCGAG 2 cut(s) 614, 1061
NsiI ATGCAT 2 cut(s) 45, 1048
NspI RCATGY 4 cut(s) 47, 87, 512, 983
OliI CACNNNNGTG 1 cut(s) 659
PceI AGGCCT 2 cut(s) 1183, 1213
PciI ACATGT 2 cut(s) 83, 979
PciSI GCTCTTC 1 cut(s) 726
PctI GAATGC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 96, 1132
PflMI CCANNNNNTGG 1 cut(s) 676
PkrI GCNGC 6 cut(s) 468, 526, 792, 811, 814, 823
PmaCI CACGTG 1 cut(s) 21
PmlI CACGTG 1 cut(s) 21
Ppu21I YACGTR 2 cut(s) 21, 1051
PscI ACATGT 2 cut(s) 83, 979
PshBI ATTAAT 1 cut(s) 564
PsiI TTATAA 1 cut(s) 368
Psp6I CCWGG 2 cut(s) 155, 461
PspCI CACGTG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 780
PspFI CCCAGC 1 cut(s) 669
PspGI CCWGG 2 cut(s) 155, 461
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 2 cut(s) 170, 992
PstI CTGCAG 1 cut(s) 529
PvuII CAGCTG 1 cut(s) 673
RsaI GTAC 1 cut(s) 978
RsaNI GTAC 1 cut(s) 977
RseI CAYNNNNRTG 2 cut(s) 659, 984
SacII CCGCGG 1 cut(s) 794
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 4 cut(s) 228, 564, 759, 1016
SatI GCNGC 6 cut(s) 467, 525, 791, 810, 813, 822
Sau3AI GATC 4 cut(s) 63, 100, 137, 595
Sau96I GGNCC 2 cut(s) 170, 992
ScrFI CCNGG 2 cut(s) 157, 463
SexAI ACCWGGT 1 cut(s) 155
SfaNI GCATC 2 cut(s) 261, 1033
SfcI CTRYAG 2 cut(s) 525, 550
Sfr303I CCGCGG 1 cut(s) 794
SgrBI CCGCGG 1 cut(s) 794
SinI GGWCC 2 cut(s) 170, 992
SmiMI CAYNNNNRTG 2 cut(s) 659, 984
Sse9I AATT 9 cut(s) 203, 241, 369, 402, 519, 685, 882, 1017, 1114
SseBI AGGCCT 2 cut(s) 1183, 1213
SsiI CCGC 6 cut(s) 427, 791, 793, 812, 998, 1126
SspI AATATT 1 cut(s) 1108
StuI AGGCCT 2 cut(s) 1183, 1213
StyD4I CCNGG 2 cut(s) 155, 461
StyI CCWWGG 2 cut(s) 742, 1235
TaaI ACNGT 4 cut(s) 297, 551, 613, 1148
TaiI ACGT 4 cut(s) 23, 632, 873, 1053
TaqI TCGA 3 cut(s) 94, 99, 136
TasI AATT 9 cut(s) 203, 241, 369, 402, 519, 685, 882, 1017, 1114
TauI GCSGC 2 cut(s) 793, 815
TfiI GAWTC 2 cut(s) 96, 1132
Tru1I TTAA 4 cut(s) 228, 564, 759, 1016
Tru9I TTAA 4 cut(s) 228, 564, 759, 1016
TscAI CASTG 3 cut(s) 300, 313, 852
TseI GCWGC 4 cut(s) 466, 524, 809, 821
TspDTI ATGAA 7 cut(s) 197, 292, 426, 550, 582, 919, 1179
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 3 cut(s) 300, 313, 852
Van91I CCANNNNNTGG 1 cut(s) 676
VpaK11BI GGWCC 2 cut(s) 170, 992
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 2 cut(s) 241, 685
XceI RCATGY 4 cut(s) 47, 87, 512, 983
Zsp2I ATGCAT 2 cut(s) 45, 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.