Rroxscaffold_3G00229250

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013147
Physical Location & Seq
Reverse (-)
13740107 .. 13743696
3590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229250.1

Sequence Viewer

Length: 1098 bp
ATGGATTTGCTTAACTCGGTTCTGAATTTCGTGGTGCCACCGGCAAGTTTGGTGATGCTGGCTTTTTCTTGGCCTGCACTGTGCTTCATCAGTGGTTGCGAGTGGCTTTACAACCAGTTCAATGCTGAAGATATGGAGGACAAAGTGGTTATAATTACTGGAGCTTCTTCTGGCATAGGGGAGCAAATTGCATATGAATATGCAAAGAGGCGGGCGAAGCTTGTCTTAGTGGCAAGAAGAGAGACCAGGCTGCGAGGGATTGGTGAGAATGCAAGGTTCTTGGGTGCAAAGCATGTCTTGATAATTGCTGCAGATGTTGTCAAGGAGGAGGACTGTAGGAGATTCATTAATGAAACCATAAACATTTACGGCCGAGAAACAGTTAGCATTCATGTGGATCATCTTGTAAACACAGTAAGCTTAGGACACACGTTCAGCTTTGAGGAAGTTACAGACACTTCTGTGTTTCCCCAGCTGTTGGACATAAATTTTTGGGGAAATGTTTATCCTACATATGTTGCTCTTCCTTACCTGCGTCAAACCCATGGAAAAGTCATTGTTAATGCCTCAGTTGAGAGCTGGTTACCTTTGCCGCAGATGAGTTTATATGCTGCGGCAAAGGCAGCTCTGGTCAACTTTTATGAGACACTGAGATTAGAAGTAAAAGACAACGTGGGGATAACAATTGCAACTCATGGATGGATTGTTAGTGAAATGTCAAGAGGCAAGTTCATGGTAGATGAGGGTGCTGAAATGCAATGGAAGGAAGAAAGAGAGGTACATGTGAGTGGTGGACCGGTGGAGGATTTTGCAAGGTTAATTGTAGCAGGGGCTTGCCGAGGAGATGCATACGTGAAGTATCCAAGCTGGTATGACATATTCCTGCTTTACAGGGTGTTTGCACCTAATATTCTGAATTGGACATTCCGCATGATTCTTCCTACACACGGTATGAGGAGAACTTCACTCATTGGCACGGGGAGGCCTATTTCATCTGAAGGTAGCTATGGAAGGCCTTTTTTGGAAGGAACTTCACCAAGGAGACTTCTTGTTAGTCCAACTTTCACTCCACTCAGTCATCAGCACAAACTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

365

Amino Acids

41.2

Weight (kDa)

6.38

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 48 - 241 1.3e-32 short chain dehydrogenase
adh_short_C2 PF13561 54 - 242 1e-16 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 540
AccB1I GGYRCC 1 cut(s) 34
AccB7I CCANNNNNTGG 1 cut(s) 478
AciI CCGC 4 cut(s) 211, 593, 614, 928
AclWI GGATC 1 cut(s) 405
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 2 cut(s) 25, 487
AcuI CTGAAG 2 cut(s) 147, 1017
AfaI GTAC 1 cut(s) 780
AfiI CCNNNNNNNGG 2 cut(s) 478, 947
AflIII ACRYGT 2 cut(s) 429, 781
AgeI ACCGGT 1 cut(s) 796
AgsI TTSAA 1 cut(s) 121
AjnI CCWGG 1 cut(s) 245
AleI CACNNNNGTG 1 cut(s) 461
AluBI AGCT 9 cut(s) 164, 220, 420, 438, 475, 579, 626, 867, 1005
AluI AGCT 9 cut(s) 164, 220, 420, 438, 475, 579, 626, 867, 1005
Alw26I GTCTC 3 cut(s) 236, 638, 1036
AlwI GGATC 1 cut(s) 405
AoxI GGCC 4 cut(s) 71, 370, 983, 1013
ApeKI GCWGC 4 cut(s) 250, 308, 611, 623
ApoI RAATTY 2 cut(s) 25, 487
AseI ATTAAT 1 cut(s) 348
AsiGI ACCGGT 1 cut(s) 796
AspS9I GGNCC 1 cut(s) 794
AsuHPI GGTGA 3 cut(s) 64, 275, 1026
AvaII GGWCC 1 cut(s) 794
BanI GGYRCC 1 cut(s) 34
BbvI GCAGC 4 cut(s) 237, 295, 598, 635
BccI CCATC 1 cut(s) 693
BceAI ACGGC 1 cut(s) 385
BciT130I CCWGG 1 cut(s) 247
BciVI GTATCC 1 cut(s) 870
BcoDI GTCTC 3 cut(s) 236, 638, 1036
BfmI CTRYAG 2 cut(s) 309, 334
BfuAI ACCTGC 1 cut(s) 540
BfuI GTATCC 1 cut(s) 870
BisI GCNGC 6 cut(s) 251, 309, 593, 612, 615, 624
BlsI GCNGC 6 cut(s) 252, 310, 594, 613, 616, 625
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 1 cut(s) 794
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 247
BmsI GCATC 2 cut(s) 45, 835
BpmI CTGGAG 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 421
BsaAI YACGTR 1 cut(s) 853
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 3 cut(s) 544, 838, 1037
BsaWI WCCGGW 1 cut(s) 796
BsaXI ACNNNNNCTCC 2 cut(s) 1051, 1081
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 947
Bse118I RCCGGY 2 cut(s) 40, 796
Bse1I ACTGG 3 cut(s) 115, 163, 1095
Bse3DI GCAATG 1 cut(s) 764
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 3 cut(s) 544, 838, 1037
BseGI GGATG 1 cut(s) 704
BseLI CCNNNNNNNGG 2 cut(s) 478, 947
BseMI GCAATG 1 cut(s) 764
BseMII CTCAG 3 cut(s) 582, 641, 1087
BseNI ACTGG 3 cut(s) 115, 163, 1095
BseRI GAGGAG 3 cut(s) 341, 855, 970
BseX3I CGGCCG 1 cut(s) 370
BseXI GCAGC 4 cut(s) 237, 295, 598, 635
BseYI CCCAGC 1 cut(s) 471
BsgI GTGCAG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 4 cut(s) 73, 372, 985, 1015
BshNI GGYRCC 1 cut(s) 34
BshTI ACCGGT 1 cut(s) 796
BsiEI CGRYCG 1 cut(s) 373
BsiSI CCGG 2 cut(s) 41, 797
BslI CCNNNNNNNGG 2 cut(s) 478, 947
BsmAI GTCTC 3 cut(s) 236, 638, 1036
BsmI GAATGC 2 cut(s) 274, 387
BsnI GGCC 4 cut(s) 73, 372, 985, 1015
Bso31I GGTCTC 1 cut(s) 236
Bsp143I GATC 1 cut(s) 397
Bsp19I CCATGG 1 cut(s) 544
BspACI CCGC 4 cut(s) 211, 593, 614, 928
BspANI GGCC 4 cut(s) 73, 372, 985, 1015
BspCNI CTCAG 3 cut(s) 581, 642, 1086
BspLI GGNNCC 1 cut(s) 36
BspMAI CTGCAG 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 540
BspPI GGATC 1 cut(s) 405
BspQI GCTCTTC 1 cut(s) 528
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 764
BsrFI RCCGGY 2 cut(s) 40, 796
BsrI ACTGG 3 cut(s) 115, 163, 1095
BssAI RCCGGY 2 cut(s) 40, 796
BssECI CCNNGG 3 cut(s) 544, 838, 1037
BssMI GATC 1 cut(s) 397
BssT1I CCWWGG 2 cut(s) 544, 1037
Bst2UI CCWGG 1 cut(s) 247
Bst4CI ACNGT 5 cut(s) 81, 335, 382, 415, 950
Bst6I CTCTTC 2 cut(s) 232, 528
BstBAI YACGTR 1 cut(s) 853
BstC8I GCNNGC 4 cut(s) 60, 75, 213, 835
BstDEI CTNAG 5 cut(s) 226, 421, 568, 650, 1073
BstDSI CCRYGG 1 cut(s) 544
BstEII GGTNACC 1 cut(s) 582
BstF5I GGATG 1 cut(s) 704
BstKTI GATC 1 cut(s) 400
BstMAI GTCTC 3 cut(s) 236, 638, 1036
BstMBI GATC 1 cut(s) 397
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 3 cut(s) 217, 620, 623
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 2 cut(s) 296, 785
BstPI GGTNACC 1 cut(s) 582
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 2 cut(s) 309, 334
BstV1I GCAGC 4 cut(s) 237, 295, 598, 635
BstZI CGGCCG 1 cut(s) 370
BsuI GTATCC 1 cut(s) 870
BsuRI GGCC 4 cut(s) 73, 372, 985, 1015
BtgI CCRYGG 1 cut(s) 544
BtsCI GGATG 1 cut(s) 704
BtsIMutI CAGTG 3 cut(s) 77, 97, 647
BveI ACCTGC 1 cut(s) 540
Cac8I GCNNGC 4 cut(s) 60, 75, 213, 835
Cfr10I RCCGGY 2 cut(s) 40, 796
Cfr13I GGNCC 1 cut(s) 794
CseI GACGC 1 cut(s) 524
Csp6I GTAC 1 cut(s) 779
CspAI ACCGGT 1 cut(s) 796
CviAII CATG 7 cut(s) 293, 392, 545, 695, 733, 782, 931
CviQI GTAC 1 cut(s) 779
DdeI CTNAG 5 cut(s) 226, 421, 568, 650, 1073
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 370
EagI CGGCCG 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 232, 528
EarI CTCTTC 2 cut(s) 232, 528
EclXI CGGCCG 1 cut(s) 370
Eco130I CCWWGG 2 cut(s) 544, 1037
Eco147I AGGCCT 2 cut(s) 985, 1015
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 794
Eco52I CGGCCG 1 cut(s) 370
Eco57I CTGAAG 2 cut(s) 147, 1017
Eco91I GGTNACC 1 cut(s) 582
EcoO65I GGTNACC 1 cut(s) 582
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 544, 1037
EcoT22I ATGCAT 1 cut(s) 850
ErhI CCWWGG 2 cut(s) 544, 1037
FaeI CATG 7 cut(s) 296, 395, 548, 698, 736, 785, 934
FalI AAGNNNNNCTT 4 cut(s) 209, 241, 281, 313
FatI CATG 7 cut(s) 292, 391, 544, 694, 732, 781, 930
FauI CCCGC 1 cut(s) 204
FauNDI CATATG 2 cut(s) 193, 514
Fnu4HI GCNGC 6 cut(s) 251, 309, 593, 612, 615, 624
FokI GGATG 1 cut(s) 711
Fsp4HI GCNGC 6 cut(s) 251, 309, 593, 612, 615, 624
GluI GCNGC 6 cut(s) 251, 309, 593, 612, 615, 624
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 4 cut(s) 73, 372, 985, 1015
HapII CCGG 2 cut(s) 41, 797
HgaI GACGC 1 cut(s) 524
Hin1II CATG 7 cut(s) 296, 395, 548, 698, 736, 785, 934
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 2 cut(s) 218, 418
HinfI GANTC 2 cut(s) 342, 934
HpaII CCGG 2 cut(s) 41, 797
HphI GGTGA 3 cut(s) 64, 275, 1026
Hpy166II GTNNAC 3 cut(s) 409, 634, 794
Hpy188I TCNGA 3 cut(s) 24, 915, 997
Hpy188III TCNNGA 2 cut(s) 298, 720
Hpy8I GTNNAC 3 cut(s) 409, 634, 794
HpyAV CCTTC 4 cut(s) 757, 992, 1005, 1019
HpyCH4III ACNGT 5 cut(s) 81, 335, 382, 415, 950
HpyCH4IV ACGT 3 cut(s) 431, 672, 852
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 620, 623
HpyF3I CTNAG 5 cut(s) 226, 421, 568, 650, 1073
HpySE526I ACGT 3 cut(s) 431, 672, 852
Hsp92II CATG 7 cut(s) 296, 395, 548, 698, 736, 785, 934
Kzo9I GATC 1 cut(s) 397
LguI GCTCTTC 1 cut(s) 528
LmnI GCTCC 2 cut(s) 161, 181
Lsp1109I GCAGC 4 cut(s) 237, 295, 598, 635
LweI GCATC 2 cut(s) 45, 835
MaeII ACGT 3 cut(s) 431, 672, 852
MaeIII GTNAC 2 cut(s) 448, 582
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 6 cut(s) 140, 159, 249, 515, 779, 929
MfeI CAATTG 1 cut(s) 684
MluCI AATT 8 cut(s) 25, 153, 186, 303, 487, 684, 819, 916
MmeI TCCRAC 2 cut(s) 459, 1082
Mph1103I ATGCAT 1 cut(s) 850
MseI TTAA 4 cut(s) 12, 348, 561, 818
MslI CAYNNNNRTG 3 cut(s) 392, 461, 786
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 2 cut(s) 41, 797
MspR9I CCNGG 1 cut(s) 247
MunI CAATTG 1 cut(s) 684
Mva1269I GAATGC 2 cut(s) 274, 387
MvaI CCWGG 1 cut(s) 247
MwoI GCNNNNNNNGC 3 cut(s) 217, 620, 623
NcoI CCATGG 1 cut(s) 544
NdeI CATATG 2 cut(s) 193, 514
NdeII GATC 1 cut(s) 397
NlaIII CATG 7 cut(s) 296, 395, 548, 698, 736, 785, 934
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 2 cut(s) 398, 863
NsiI ATGCAT 1 cut(s) 850
NspI RCATGY 2 cut(s) 296, 785
OliI CACNNNNGTG 1 cut(s) 461
PceI AGGCCT 2 cut(s) 985, 1015
PciI ACATGT 1 cut(s) 781
PciSI GCTCTTC 1 cut(s) 528
PctI GAATGC 2 cut(s) 274, 387
PfeI GAWTC 2 cut(s) 342, 934
PflMI CCANNNNNTGG 1 cut(s) 478
PinAI ACCGGT 1 cut(s) 796
PkrI GCNGC 6 cut(s) 252, 310, 594, 613, 616, 625
Ppu21I YACGTR 1 cut(s) 853
PscI ACATGT 1 cut(s) 781
PshBI ATTAAT 1 cut(s) 348
PsiI TTATAA 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 582
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 794
PstI CTGCAG 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 1 cut(s) 780
RsaNI GTAC 1 cut(s) 779
RseI CAYNNNNRTG 3 cut(s) 392, 461, 786
SapI GCTCTTC 1 cut(s) 528
SaqAI TTAA 4 cut(s) 12, 348, 561, 818
SatI GCNGC 6 cut(s) 251, 309, 593, 612, 615, 624
Sau3AI GATC 1 cut(s) 397
Sau96I GGNCC 1 cut(s) 794
ScrFI CCNGG 1 cut(s) 247
SfaNI GCATC 2 cut(s) 45, 835
SfcI CTRYAG 2 cut(s) 309, 334
SinI GGWCC 1 cut(s) 794
SmiMI CAYNNNNRTG 3 cut(s) 392, 461, 786
Sse9I AATT 8 cut(s) 25, 153, 186, 303, 487, 684, 819, 916
SseBI AGGCCT 2 cut(s) 985, 1015
SsiI CCGC 4 cut(s) 211, 593, 614, 928
SspI AATATT 1 cut(s) 910
StuI AGGCCT 2 cut(s) 985, 1015
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 2 cut(s) 544, 1037
TaaI ACNGT 5 cut(s) 81, 335, 382, 415, 950
TaiI ACGT 3 cut(s) 434, 675, 855
TasI AATT 8 cut(s) 25, 153, 186, 303, 487, 684, 819, 916
TauI GCSGC 2 cut(s) 595, 617
TfiI GAWTC 2 cut(s) 342, 934
Tru1I TTAA 4 cut(s) 12, 348, 561, 818
Tru9I TTAA 4 cut(s) 12, 348, 561, 818
TscAI CASTG 3 cut(s) 84, 97, 654
TseI GCWGC 4 cut(s) 250, 308, 611, 623
TspDTI ATGAA 7 cut(s) 76, 210, 334, 366, 380, 721, 981
TspRI CASTG 3 cut(s) 84, 97, 654
Van91I CCANNNNNTGG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 794
VspI ATTAAT 1 cut(s) 348
XapI RAATTY 2 cut(s) 25, 487
XceI RCATGY 2 cut(s) 296, 785
Zsp2I ATGCAT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.